CN106107366A - The preparation method of a kind of Phaeoporus obliquus oral liquid and special culture media - Google Patents
The preparation method of a kind of Phaeoporus obliquus oral liquid and special culture media Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
- A23L2/382—Other non-alcoholic beverages fermented
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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Abstract
The invention discloses preparation method and the special culture media of a kind of Phaeoporus obliquus oral liquid, fundamentally solve and fill up, with biofermentation technique, the present situation that wild Phaeoporus obliquus is not enough, also improve the content of Phaeoporus obliquus main component in oral liquid.Culture medium is based on natural plants, and natural green is without chemical residual, and trace element is many, comprehensive nutrition；Glucose adds jointly with the disaccharide of one of sucrose, white sugar, brown sugar, allows composition comprehensive and lasting；3, traditional chemical defoamer is substituted with vegetable oil, the most nutritious, again without chemical residual；4, oral liquid is producing and in processing pouring process, omnidistance do not use high temperature, be dried, extract, crush etc. traditional or modern either physically or chemically, the most only effectively remain all of active component in oral liquid, and make active component not be damaged or destroyed；From preparation shaking flask kind to product fill all without additives such as any preservative, thickening agent, stabilizer, coloring agent, this oral liquid active constituent content is high.
Description
Technical field
The present invention relates to the preparation method of a kind of oral liquid, the preparation method of a kind of Phaeoporus obliquus oral liquid and special
Culture medium.
Background technology
Phaeoporus obliquus is the another name of Inonqqus obliquus, is a kind of large-scale parastic fungus being born on Phaeoporus obliquus tree.Main distribution
In Russia the north, Northern Europe, the Heilungkiang of China, Jilin Area.
Phaeoporus obliquus has centuries in Russia's Popular Utilization history, is known as " catholicon ", and the U.S. arranges Phaeoporus obliquus
For " special natural materials ", the beverage of human future.
Effect of Phaeoporus obliquus causes extensive attention all over the world, is proved to have treatment kinds cancer, heart disease, glycosuria
Disease and the effect such as antiviral, blood pressure lowering, zoopery and clinical experiment that various places are long-term show to take Phaeoporus obliquus without any poison
Side effect.
But Phaeoporus obliquus wild resource is rare, growth cycle at about 10-20, artificial cultivation technique also immaturity, it is impossible to
Meet the growing market demand.
Having some patents about Phaeoporus obliquus oral liquid at present, CN103919135A discloses a kind of Phaeoporus obliquus bacterium health care
Oral liquid and preparation method thereof, by long-time soaking, broken, also have extraction etc. traditional or modern either physically or chemically,
Nutritional labeling is made to suffer substantial amounts of destruction.CN104099385A and CN102115350A etc. also disclose that Phaeoporus obliquus goods, but
Manufacturing process with the addition of the chemical addition agent such as defoamer, stabilizer.
Summary of the invention
It is an object of the invention to provide preparation method and the special culture media of a kind of Phaeoporus obliquus oral liquid, fundamentally solve
Fill up, with biofermentation technique, the present situation that wild Phaeoporus obliquus is not enough, also improve containing of Phaeoporus obliquus main component in oral liquid
Amount, for food industry and pharmaceutical industry, the health for the people provides new guarantee and approach.
The technical scheme is that and be achieved in that: the preparation method of a kind of Phaeoporus obliquus oral liquid, step is as follows:
1) shaking flask kind is prepared: culture medium loaded in conical flask, liquid amount25-35%, the temperature of 121-123 DEG C after sealing
Lower sterilizing 35 minutes, when culture medium temperature drops to 20-28 DEG C, aseptically accesses slant strains in triangular flask and shakes
Bed is cultivated, and when bacterium solution is micro-thick, bacterium solution tint lemon yellow or tenne, mycelium pellet is high-visible, and all burrs are obvious, and microscopy is without miscellaneous
During bacterium, standby；
2) fermentor cultivation: culture medium is loaded in fermentation tank, liquid amount is the 55-75% of fermentation tank total capacity, when tank temperature rise extremely
121-123 DEG C, tank pressure 0.11-0.13Mpa time, sterilizing 50min, then lead to cooling water temperature, when temperature drops to 30 DEG C in tank
Stop, adjusting tank pressure 0.05Mpa, enter cultivation conditions；
Cultivate in cultured shaking flask kind strain is accessed tank, inoculum concentration 5-15%, tank temperature 20-28 DEG C, tank pressure 0.04-
0.05MPa, gas flow 1.5m3/ h, when cultivating 36-48h in tank, pick test；
Continuing to cultivate 5-7d, when fungus ball is high-visible, all burrs are obvious, and bacterium solution is faint yellow, about pH5-6, residual sugar amount fall
During to about 0.3%, cultivation terminates, and carries out subpackage.Described subpackage is in bacteria-and dust-free workshop, uses the special fill of cold chain to set
Standby, fermentation liquid is filled to sterilized bottle.
Further, the shaking flask kind of step 1) is accessed seed tank amplification culture, then fermentor cultivation is received in expansion.
Further, described sugar is the mixing of glucose and disaccharide, and it respectively accounts for 50% mass percent.Described disaccharide
For sucrose, white sugar or brown sugar.
Further, depending on product needed or technological requirement, before subpackage, also may filter that removing fungus ball.
Further, step 1) shake-flask culture based formulas: be by weight percentage: birch 0.5-1.5%, wheat bran 0.5-
1.5%, Semen Maydis 1.5-2.5%, Semen Glycines 1.5-2.5%, Herba bromi japonici 0.5-1.5%, sugar 1.5-2.5%, yeast powder 1-2%, bee pollen 0.1-
0.2%, potassium dihydrogen phosphate 0.2%, surplus is water.
Further, in step 1) and step 2, the pH value of culture medium is 5.5-7.5.
Further, described step 2) formula of culture medium is: raw materials by weight is: birch 0.5-1.5%, bran
Skin 0.5-1.5%, Semen Maydis 1.5-2.5%, Semen Glycines 1.5-2.5%, Herba bromi japonici 0.5-1.5%, sugar 1.5-2.5%, yeast powder 1-2%, Herba leucadis ciliatae
Powder 0.1-0.2%, potassium dihydrogen phosphate 0.2%, vegetable oil 0.3-0.6%, surplus is water.
Further, shaking speed 180-240r/min in step 1), shaken cultivation 7-10 at being 20-28 DEG C cultivated by shaking table
My god.
Further, step 1) and step 2) in the preparation method of culture medium be: foreign material liquor is removed in birch sawdust sieving
Filtering, the direct liquor of bark, branch, leaves, wheat bran filters, and Semen Maydis, Semen Glycines, Herba bromi japonici are impregnated with without filter and remove residue of pulling an oar after hard-core,
Juice after filtering once adds other adjuvant, supplies water in proportion, loads in conical flask.
Further, step 2) in, when tank temperature rise to 80 DEG C, first open air bleeding valve, discharge cold air, now air bleeding valve
It is in crack state.
The invention have the benefit that 1, change the chemosynthesis culture medium in the past fermented, but with natural plants be
Main, natural green is without chemical residual, and trace element is many, comprehensive nutrition；2, conventional formulation is only added the such monosaccharide of glucose,
The disaccharide now changing glucose and one of sucrose, white sugar, brown sugar into adds jointly, allows composition comprehensive and lasting；3, traditional zymotic is used
Defoamer processes the foam that fermentation produces, and this technology vegetable oil substitutes, the most nutritious, again without chemical residual；4, oral
Liquid is producing and in processing pouring process, omnidistance does not use high temperature, is dried, extracts, traditional or modern physics or the change such as crushes
Method, the most only effectively remains all of active component in oral liquid, and makes active component not be damaged or destroyed；
5, oral liquid changes the method in the past adding the additives such as preservative, thickening agent, stabilizer, coloring agent, from preparation shaking flask kind
To product fill all without any chemical addition agent not meeting national requirements；6, the oral liquid of this kind of method production is through authority
Detection department detection active constituent content is high；7, the method using liquid fermentation, shortens the production cycle, and yield is high, and composition is good.
Detailed description of the invention
Below in conjunction with being embodied as the present invention is further explained explanation.
Embodiment 1
1, shaking flask kind formula: raw material percentage ratio by weight is: birch (sawdust, bark, leaves, branch) 0.5%, wheat bran 0.5%,
Semen Maydis 2.5%, Semen Glycines 2.5%, Herba bromi japonici 0.5%, wherein, sugar is dextrose plus saccharose to sugar 2.5%(, respectively accounts for 50%), yeast powder 1%, honeybee
Pollen 0.2%, potassium dihydrogen phosphate 0.2%, surplus is water, and pH value is 5.5.
2, method: birch sawdust sieving goes foreign material liquor to filter, the direct liquor of bark, branch, leaves, wheat bran filters,
Semen Maydis, Semen Glycines, Herba bromi japonici are impregnated with without filter and remove residue of pulling an oar after hard-core, and the juice after filtering once adds other adjuvant, in proportion
Supplying water, load in conical flask, liquid amount about 30%, by tampon (Cotton Gossypii is bound up with gauze and makes) or silica gel plug plug
Enter bottleneck, wrap laggard horizontal high voltage sterilizing with kraft paper, after emptying cold air, be warming up to 121 DEG C, timing 35min, treats that indicator is returned
Exhaust valve opening pot cover is opened when zero.When in bottle, culture medium temperature drops to 25 DEG C, aseptically with inoculation shovel by inclined-plane
Strain accesses in triangular flask, and the triangular flask having connect bacterium moves into shaking table cultivation, shaking speed 200r/min in culturing room, shakes at 25 DEG C
Swinging cultivation 8 days, when bacterium solution is micro-thick, bacterium solution tint lemon yellow or tenne, mycelium pellet is high-visible, and all burrs are obvious, microscopy
Time without miscellaneous bacteria, standby.
Two, fermentor cultivation
1, culture medium: raw material percentage ratio by weight is: birch 0.5%, wheat bran 1.5%, Semen Maydis 2.5%, Semen Glycines 1.5%, Herba bromi japonici
0.5% making beating is filtered, sugar (wherein sugar is dextrose plus saccharose, respectively accounts for 50%) 1.5%, yeast powder 1%, bee pollen 0.1%, di(2-ethylhexyl)phosphate
Hydrogen potassium 0.2%, vegetable oil 0.6%, surplus is water, and pH value is 5.5.The same Shake flask medium of preparation method.
2, liquid fermentation culturing method and condition: raw material is weighed in proportion, processing method, with shaking flask kind, stirs,
Feeding sterilizing.Liquid amount is the 60% of fermentation tank total measurement (volume).When tank temperature rise to 80 DEG C, open air bleeding valve, discharge cold air, this
Time air bleeding valve be in crack state, tank temperature rise to 121-123 DEG C, tank pressure 0.12Mpa time, timing starts, sterilizing 50min, timing
Terminating logical cooling water temperature, in tank, temperature drops to stop cooling when 30 DEG C, adjusts tank pressure 0.05Mpa, enters cultivation conditions.Will training
The shaking flask kind supported or seed tank strain are cultivated in accessing tank, inoculum concentration 10%, tank temperature 28 DEG C, tank pressure 0.04Mpa, gas
Flow 1.5m3/ h, when cultivating 36h in tank, pick test.Continuing to cultivate 6 days, when fungus ball is high-visible, all burrs are obvious, bacterium
Liquid is faint yellow, about pH5-6, residual sugar amount when being down to about 0.3%, and cultivation terminates, through checking the qualified subpackage that gets final product (during subpackage
Retain fungus ball depending on product needed or technological requirement or filter off fungus ball).
Three, subpackage, spiral cover, lamp inspection, labeling, vanning
In state specified standards purifies bacteria-and dust-free workshop, use the special filling apparatus of cold chain, fermentation liquid is filled to 30-
In the sterilized bottle of 150mL, spiral cover laggard portable lighter inspection, labeling, vanning.
Embodiment 2
1, shaking flask kind formula: raw material percentage ratio by weight is: birch (sawdust, bark, leaves, branch) 1.0%, wheat bran 1.0%,
Semen Maydis 1.5%, Semen Glycines 2%, Herba bromi japonici 1.5%, wherein, sugar is glucose and brown sugar to sugar 1.5%(, respectively accounts for 50%), yeast powder 2%, Herba leucadis ciliatae
Powder 0.1%, potassium dihydrogen phosphate 0.2%, surplus is water, and pH value is 6.5.
2, method: birch sawdust sieving goes foreign material liquor to filter, the direct liquor of bark, branch, leaves, wheat bran filters,
Semen Maydis, Semen Glycines, Herba bromi japonici are impregnated with without filter and remove residue of pulling an oar after hard-core, and the juice after filtering once adds other adjuvant, in proportion
Supplying water, load in conical flask, liquid amount about 30%, by tampon (Cotton Gossypii is bound up with gauze and makes) or silica gel plug plug
Enter bottleneck, wrap laggard horizontal high voltage sterilizing with kraft paper, after emptying cold air, be warming up to 123 DEG C, timing 35min, treats that indicator is returned
Exhaust valve opening pot cover is opened when zero.When in bottle, culture medium temperature drops to 28 DEG C, aseptically with inoculation shovel by inclined-plane
Strain accesses in triangular flask, and the triangular flask having connect bacterium moves into shaking table cultivation, shaking speed 200r/min in culturing room, shakes at 28 DEG C
Swinging cultivation 7 days, when bacterium solution is micro-thick, bacterium solution tint lemon yellow or tenne, mycelium pellet is high-visible, and all burrs are obvious, microscopy
Time without miscellaneous bacteria, standby.
Two, fermentor cultivation
1, culture medium: raw material percentage ratio by weight is: birch 1.5%, wheat bran 1 %, Semen Maydis 2%, Semen Glycines 2%, Herba bromi japonici 1% was pulled an oar
Filter, sugar (wherein sugar is dextrose plus saccharose, respectively accounts for 50%) 2%, yeast powder 1%, bee pollen 0.15%, potassium dihydrogen phosphate 0.2%, plant
Thing oil 0.4%, surplus is water, and pH value is 6.5.The same Shake flask medium of preparation method.
2, liquid fermentation culturing method and condition: raw material is weighed in proportion, processing method, with shaking flask kind, stirs,
Feeding sterilizing.Liquid amount is the 70% of fermentation tank total measurement (volume).When tank temperature rise to 80 DEG C, open air bleeding valve, discharge cold air, this
Time air bleeding valve be in crack state, when tank temperature rise to 123 DEG C, tank pressure 0.11Mpa, timing starts, sterilizing 50min, and timing terminates
Logical cooling water temperature, in tank, temperature drops to stop cooling when 30 DEG C, adjusts tank pressure 0.05Mpa, enters cultivation conditions.By cultivation well
Shaking flask kind or seed tank strain access in tank and cultivate, inoculum concentration 10%, tank temperature 28 DEG C, tank pressure 0.04Mpa, gas flow
1.5m3/ h, when cultivating 36h in tank, pick test.Continue to cultivate 7 days, when fungus ball is high-visible, week burr obvious, bacterium solution in
When faint yellow, about pH5-6, residual sugar amount are down to about 0.3%, cultivation terminates, and (regards during subpackage through the qualified subpackage that gets final product of inspection and produces
Product need or technological requirement retains fungus ball or filters off fungus ball).
Three, subpackage, spiral cover, lamp inspection, labeling, vanning
In state specified standards purifies bacteria-and dust-free workshop, use the special filling apparatus of cold chain, fermentation liquid is filled to 30-
In the sterilized bottle of 150mL, spiral cover laggard portable lighter inspection, labeling, vanning.
Embodiment 3
1, shaking flask kind formula: raw material percentage ratio by weight is: birch (sawdust, bark, leaves, branch) 1.5%, wheat bran 1.5%,
Semen Maydis 2.5%, Semen Glycines 1.5%, Herba bromi japonici 1.5%, wherein, sugar is glucose and white sugar to sugar 2.5%(, respectively accounts for 50%), yeast powder 1%, honeybee
Pollen 0.2%, potassium dihydrogen phosphate 0.2%, surplus is water, and pH value is 7.
2, method: birch sawdust sieving goes foreign material liquor to filter, the direct liquor of bark, branch, leaves, wheat bran filters,
Semen Maydis, Semen Glycines, Herba bromi japonici are impregnated with without filter and remove residue of pulling an oar after hard-core, and the juice after filtering once adds other adjuvant, in proportion
Supplying water, load in conical flask, liquid amount about 30%, by tampon (Cotton Gossypii is bound up with gauze and makes) or silica gel plug plug
Enter bottleneck, wrap laggard horizontal high voltage sterilizing with kraft paper, after emptying cold air, be warming up to 121 DEG C, timing 35min, treats that indicator is returned
Exhaust valve opening pot cover is opened when zero.When in bottle, culture medium temperature drops to 20 DEG C, aseptically with inoculation shovel by inclined-plane
Strain accesses in triangular flask, and the triangular flask having connect bacterium moves into shaking table cultivation, shaking speed 200r/min in culturing room, shakes at 20 DEG C
Swinging cultivation 10 days, when bacterium solution is micro-thick, bacterium solution tint lemon yellow or tenne, mycelium pellet is high-visible, and all burrs are obvious, microscopy
Time without miscellaneous bacteria, standby.
Two, fermentor cultivation
1, culture medium: raw material percentage ratio by weight is: birch 0.5%, wheat bran 1.5%, Semen Maydis 2.5%, Semen Glycines 1.5%, Herba bromi japonici
1.5% making beating is filtered, sugar (wherein sugar is dextrose plus saccharose, respectively accounts for 50%) 2.5%, yeast powder 1%, bee pollen 0.1%, di(2-ethylhexyl)phosphate
Hydrogen potassium 0.2%, vegetable oil 0.6%, surplus is water, and pH value is 5.5.The same Shake flask medium of preparation method.
2, liquid fermentation culturing method and condition: raw material is weighed in proportion, processing method, with shaking flask kind, stirs,
Feeding sterilizing.Liquid amount is the 55% of fermentation tank total measurement (volume).When tank temperature rise to 80 DEG C, open air bleeding valve, discharge cold air, this
Time air bleeding valve be in crack state, tank temperature rise to 121-123 DEG C, tank pressure 0.13Mpa time, timing starts, sterilizing 50min, timing
Terminating logical cooling water temperature, in tank, temperature drops to stop cooling when 30 DEG C, adjusts tank pressure 0.05Mpa, enters cultivation conditions.Will training
The shaking flask kind supported or seed tank strain are cultivated in accessing tank, inoculum concentration 10%, tank temperature 28 DEG C, tank pressure 0.05Mpa, gas
Flow 1.5m3/ h, when cultivating 36h in tank, pick test.Continuing to cultivate 5 days, when fungus ball is high-visible, all burrs are obvious, bacterium
Liquid is faint yellow, about pH5-6, residual sugar amount when being down to about 0.3%, and cultivation terminates, through checking the qualified subpackage that gets final product (during subpackage
Retain fungus ball depending on product needed or technological requirement or filter off fungus ball).
Three, subpackage, spiral cover, lamp inspection, labeling, vanning
In state specified standards purifies bacteria-and dust-free workshop, use the special filling apparatus of cold chain, fermentation liquid is filled to 30-
In the sterilized bottle of 150mL, spiral cover laggard portable lighter inspection, labeling, vanning.
The above, the only present invention preferably detailed description of the invention, but protection scope of the present invention is not limited thereto,
Any those familiar with the art in the technical scope of present disclosure, according to technical scheme and
Inventive concept equivalent or change in addition, all should contain within protection scope of the present invention.
Claims (10)
1. the preparation method of a Phaeoporus obliquus oral liquid, it is characterised in that step is as follows:
1) shaking flask kind is prepared:
Culture medium is loaded in conical flask, liquid amount 25-35%, after sealing at a temperature of 121-123 DEG C sterilizing 35 minutes,
When culture medium temperature drops to 20-28 DEG C, in slant strains aseptically accesses triangular flask, upper shaking table is cultivated, and works as bacterium solution
Micro-thick, bacterium solution tint lemon yellow or tenne, mycelium pellet is high-visible, and all burrs are obvious, when microscopy is without miscellaneous bacteria, standby；
2) fermentor cultivation:
Culture medium being loaded in fermentation tank, liquid amount is the 55-75% of fermentation tank total capacity, when tank temperature rise is to 121-123 DEG C, tank
During pressure 0.11-0.13Mpa, sterilizing 50min, then lead to cooling water temperature, in tank, temperature drops to stop when 30 DEG C, adjusts tank pressure
To 0.05MPa, enter cultivation conditions；
Cultivate in cultured shaking flask kind strain is accessed tank, inoculum concentration 5-15%, tank temperature 20-28 DEG C, tank pressure 0.04-
0.05MPa, gas flow 1.5m3/ h, when cultivating 36-48h in tank, pick test；
Continuing to cultivate 5-7 days, when fungus ball is high-visible, all burrs are obvious, and bacterium solution is faint yellow, about pH5-6, residual sugar amount fall
During to about 0.3%, cultivation terminates, and carries out subpackage.
The preparation method of Phaeoporus obliquus oral liquid the most according to claim 1, it is characterised in that the shaking flask kind of step 1) is connect
Enter seed tank amplification culture, then fermentor cultivation is received in expansion.
The preparation method of Phaeoporus obliquus oral liquid the most according to claim 1, it is characterised in that be filtered to remove bacterium before subpackage
Ball.
4. shaking flask kind special culture media in the preparation method of a kind of Phaeoporus obliquus oral liquid as described in any one of claim 1-3,
It is characterized in that, be by weight percentage: birch 0.5-1.5%, wheat bran 0.5-1.5%, Semen Maydis 1.5-2.5%, Semen Glycines 1.5-
2.5%, Herba bromi japonici 0.5-1.5%, sugar 1.5-2.5%, yeast powder 1-2%, bee pollen 0.1-0.2%, potassium dihydrogen phosphate 0.2%, surplus is
Water.
5. the special cultivation of fermentor cultivation in the preparation method of a kind of Phaeoporus obliquus oral liquid as described in any one of claim 1-3
Base, it is characterised in that raw materials by weight is: birch 0.5-1.5%, wheat bran 0.5-1.5%, Semen Maydis 1.5-2.5%, Semen Glycines
1.5-2.5%, Herba bromi japonici 0.5-1.5%, sugar 1.5-2.5%, yeast powder 1-2%, bee pollen 0.1-0.2%, potassium dihydrogen phosphate 0.2%, plant
Thing oil 0.3-0.6%, surplus is water.
6. according to the preparation method of the Phaeoporus obliquus oral liquid described in claim 4 or 5, it is characterised in that described sugar is Fructus Vitis viniferae
Sugar and the mixing of disaccharide, it respectively accounts for 50% mass percent, and described disaccharide is sucrose, white sugar or brown sugar.
7. according to the preparation method of the Phaeoporus obliquus oral liquid described in claim 4 or 5, it is characterised in that the pH value of culture medium is
5.5-7.5。
The preparation method of Phaeoporus obliquus oral liquid the most according to claim 1, it is characterised in that shaking speed in step 1)
180-240r/min, shaken cultivation 7-10 days at being 20-28 DEG C cultivated by shaking table.
9. according to the preparation method of the Phaeoporus obliquus oral liquid described in claim 4 or 6, it is characterised in that step 1) and step 2)
The preparation method of middle culture medium is: birch sawdust sieving goes foreign material liquor to filter, the direct liquor of bark, branch, leaves, wheat bran
Filtering, Semen Maydis, Semen Glycines, Herba bromi japonici are impregnated with without filter and remove residue of pulling an oar after hard-core, and the juice after filtering once adds other adjuvant, presses
Ratio supplies water, loads in conical flask.
The preparation method of Phaeoporus obliquus oral liquid the most according to claim 1, it is characterised in that step 2) in, when tank temperature rise
During to 80 DEG C, first opening air bleeding valve, discharge cold air, now air bleeding valve is in crack state.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021017449A1 (en) * | 2019-07-29 | 2021-02-04 | 浙江养生堂天然药物研究所有限公司 | Fermented brich juice, production method therefor and use thereof in composition for external application to skin |
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| Publication number | Priority date | Publication date | Assignee | Title |
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Application publication date: 20161116 |