CN106106884A - A kind of Antrodia camphorata fermented tea and preparation method thereof - Google Patents
A kind of Antrodia camphorata fermented tea and preparation method thereof Download PDFInfo
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- CN106106884A CN106106884A CN201610454186.7A CN201610454186A CN106106884A CN 106106884 A CN106106884 A CN 106106884A CN 201610454186 A CN201610454186 A CN 201610454186A CN 106106884 A CN106106884 A CN 106106884A
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- antrodia camphorata
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/06—Treating tea before extraction; Preparations produced thereby
- A23F3/14—Tea preparations, e.g. using additives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/06—Treating tea before extraction; Preparations produced thereby
- A23F3/08—Oxidation; Fermentation
- A23F3/10—Fermentation with addition of microorganisms or enzymes
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Abstract
The invention provides the preparation method of a kind of Antrodia camphorata fermented tea, comprise the following steps: step one, Antrodia camphorata bacterial strain S 29 is cultivated in malt extract medium, obtain the Antrodia camphorata strain activated；Step 2, is linked into the Antrodia camphorata strain of activation in the liquid seed culture medium after sterilizing, cultivates and obtains liquid strain；Step 3, joins carbon source fermentation in Folium Camelliae sinensis and prepares Folium Camelliae sinensis solid medium；Step 4, joins tap water in Folium Camelliae sinensis solid-state fermentation culture medium and soaks, the Folium Camelliae sinensis solid-state fermentation culture medium after being soaked；Step 5, is linked into liquid strain in the Folium Camelliae sinensis solid-state fermentation culture medium after immersion cultivation, obtains Antrodia camphorata fermented tea crude product；Step 6, puts into baking oven by Antrodia camphorata fermented tea crude product, pulverizes after drying, packs, obtain Antrodia camphorata fermented tea after loading tea bag.The Antrodia camphorata fermented tea prepared by preparation method of the present invention has containing abundant active component in strong fragranced, and Antrodia camphorata fermented tea, is a kind of good health promoting beverage.
Description
Technical field
The invention belongs to technical field of microbial fermentation, be specifically related to a kind of Antrodia camphorata fermented tea and the system of this Antrodia camphorata fermented tea
Preparation Method.
Background technology
Antrodia camphorata is the food and medicament dual-purpose fungus that the distinctive class in China Taiwan is the most precious, can produce triterpene, polysaccharide, steroid
The various active composition such as alcohol, benzene ring type compounds, is used as the good medicine for the treatment of disease by Taiwan aborigines.In recent years, along with right
Going deep into of Antrodia camphorata research, increasing biological active substances is by isolation identification.Numerous researchs show, Antrodia camphorata has solution bartender
The pharmacologically actives, especially Antrodins such as liver, antitumor, antiviral, antioxidation, anti-inflammatory and immunomodulating and
Antroquinonol compounds, protecting the liver, anticancer aspect there is the curative effect of brilliance.
Fermented tea refers to by microbial action and hydrothermal promotes polyphenols fully to carry out autoxidation, thus
Formed fragrance alcohol and, the teas that soup color depth is red, fermented tea is one of big teas of China six, the big tea of Ye Shi China distinctive one
Class, its production history is long, such as Folium camelliae assamicae, brick tea etc..Numerous studies confirm, fermented tea have stronger promoting digestion greasy, relieve the effect of alcohol,
Blood fat reducing, recover from fatigue the plurality of health care functions such as relieving restlessness.
Traditional fermented tea is natural fermentation process, belongs to semi open model fermentation, participates in microbe species various, it is difficult to control
System, causing various active composition in Folium Camelliae sinensis cannot be constant, such as tea polyphenols, caffeine, and, during the fermentation by the external world
Factor impact is relatively big, and the fermented tea inferior quality obtained, local flavor is the best.
Summary of the invention
For solving the problems referred to above, the present invention, by providing the preparation method of a kind of Antrodia camphorata fermented tea, develops one and has
Strong Antrodia camphorata fragrance and the Antrodia camphorata fermented tea of abundant active component.
The technical solution used in the present invention is as follows:
The invention provides a kind of Antrodia camphorata fermented tea, have a feature in that in every 100g Antrodia camphorata fermented tea containing Antrodia camphorata
Bacterium 8.62～15.77g and active polysaccharide 4.42～6.61g.
Present invention also offers the preparation method of a kind of Antrodia camphorata fermented tea, use Antrodia camphorata bacterial strain (Antrodia
Camphorata) S-29 is prepared, and this bacterial strain has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms
Center, deposit number is CGMCC No.9590, and preparation method comprises the following steps:
Step one, cultivates Antrodia camphorata bacterial strain S-29 predetermined number of days in malt extract medium, obtains the Antrodia camphorata strain activated；
Step 2, is linked into the Antrodia camphorata strain of activation in the liquid seed culture medium after sterilizing, is 20～30 in temperature
Under conditions of DEG C, cultivate and obtain liquid strain in 2～6 days；
2～15g carbon sources are joined in 100g Folium Camelliae sinensis and the after fermentation that stirs prepare Folium Camelliae sinensis solid state rheology by step 3
Base；
Step 4, joins a certain amount of water in Folium Camelliae sinensis solid-state fermentation culture medium, in the condition that temperature is 15～22 DEG C
Under, soak 2～6 hours, the Folium Camelliae sinensis solid-state fermentation culture medium after being soaked；
Step 5, is linked into liquid strain in the Folium Camelliae sinensis solid-state fermentation culture medium after immersion by predetermined ratio, in temperature
Under conditions of being 20～30 DEG C, cultivate 12～25 days, obtain Antrodia camphorata fermented tea crude product；
Step 6, puts into baking oven by Antrodia camphorata fermented tea crude product, under the conditions of temperature is 20～60 DEG C, pulverizes after drying,
Pack after loading tea bag, obtain Antrodia camphorata fermented tea.
In the preparation method of the Antrodia camphorata fermented tea of present invention offer, it is also possible to have the feature that, wherein, in step
In one, predetermined number of days is 12 days.
In the preparation method of the Antrodia camphorata fermented tea of present invention offer, it is also possible to have the feature that, wherein, in step
In two, the composition of liquid seed culture medium is: every 1L liquid seed culture medium contains glucose 20g, analysis for soybean powder 4g and Semen Maydis
Starch 2g, liquid seed culture medium is sterilizing 20min at 115 DEG C.
In the preparation method of the Antrodia camphorata fermented tea of present invention offer, it is also possible to have the feature that, wherein, in step
In three, Folium Camelliae sinensis is any 1 in black tea, green tea, postfermented tea and oolong tea～4 kind.
In the preparation method of the Antrodia camphorata fermented tea of present invention offer, it is also possible to have the feature that, wherein, in step
In three, carbon source is any 1 in glucose, fructose, maltose, lactose and sucrose～5 kind.
In the preparation method of the Antrodia camphorata fermented tea of present invention offer, it is also possible to have the feature that, wherein, in step
In three, carbon source is any 1 in rice meal, millet powder, oatmeal, Semen avenae nudae powder, Semen Maydis powder～5 kind.
In the preparation method of the Antrodia camphorata fermented tea of present invention offer, it is also possible to have the feature that, wherein, in step
In four, every 100g Folium Camelliae sinensis solid-state fermentation culture medium adds 40～100ml water.
In the preparation method of the Antrodia camphorata fermented tea of present invention offer, it is also possible to have the feature that, wherein, in step
In five, predetermined ratio is the Folium Camelliae sinensis solid-state fermentation culture medium after soaking and liquid strain ratio 100:15～45 (g/ml).
The effect of invention and effect
The invention provides the preparation method of a kind of Antrodia camphorata fermented tea, fragranced strong for Antrodia camphorata bacterium is incorporated into Folium Camelliae sinensis
In, enriching the odor type of Folium Camelliae sinensis, the preparation method process of the present invention is simple, easy to control, and production efficiency is high, easily realizes extensive
Production, there is important industrial application value, the Antrodia camphorata fermented tea quality using the preparation method of the present invention to prepare is high, has
Strong fragranced, and, containing abundant active component in Antrodia camphorata fermented tea, it is a kind of good health promoting beverage.
Detailed description of the invention
The present invention is expanded on further below in conjunction with embodiment, for concrete grammar used in embodiment or material, this
Skilled person on the basis of the technology of the present invention thinking, can carry out the replacement of routine and select according to existing technology, and
It is not limited only to the concrete record of the embodiment of the present invention.
Test method used in embodiment if no special instructions, is accordingly to be regarded as conventional method；The material that used, reagent
Deng, if no special instructions, the most commercially obtain.
Malt extract medium: beerwort 150ml, agar 3g, pH are natural (about 6.4)
Liquid seed culture medium: every 1L liquid seed culture medium contains glucose 20g, analysis for soybean powder 4g and Semen Maydis starch
2g, liquid seed culture medium is sterilizing 20min at 115 DEG C.
Folium Camelliae sinensis solid medium: containing Folium Camelliae sinensis and carbon source, Folium Camelliae sinensis be in black tea, green tea, postfermented tea and oolong tea any 1～
Making for 4 kinds, carbon source is in glucose, fructose, maltose, lactose and sucrose any 1～5 kind, or rice meal, millet powder, swallow
In flour, Semen avenae nudae powder and Semen Maydis powder any 1～5 kind.
Antrodia camphorata bacterial strain (Antrodia camphorata) S-29 was preserved in China Microbiological bacterium on 09 09th, 2014
Plant preservation administration committee common micro-organisms center (address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3), its deposit number
For CGMCC No.9590.
Embodiment one
The Antrodia camphorata fermented tea preparation method of the present embodiment comprises the following steps:
Step one, cultivates ready Antrodia camphorata bacterial strain S-29 in malt extract medium under conditions of temperature is 28 DEG C
12 days, obtain the Antrodia camphorata strain activated；
Step 2, is linked into the Antrodia camphorata strain of activation in the liquid seed culture medium after sterilizing, is 20 DEG C of bars in temperature
Under part, cultivate and obtain liquid strain in 2 days；
Step 3, prepares Folium Camelliae sinensis solid medium after 2g glucose is joined in 100g black tea and stirred；
Step 4, joins the 100g Folium Camelliae sinensis solid state fermentation culture after at 115 DEG C sterilizing 15 minutes by 40ml tap water
In base, under conditions of temperature is 15 DEG C, soak 2 hours, the Folium Camelliae sinensis solid-state fermentation culture medium after being soaked；
Step 5, is linked into 15ml liquid strain in the Folium Camelliae sinensis solid-state fermentation culture medium after immersion, is 20 DEG C in temperature
Under the conditions of, cultivate 12 days, obtain Antrodia camphorata fermented tea crude product；
Step 6, puts into baking oven by Antrodia camphorata fermented tea crude product, under the conditions of temperature is 20 DEG C, dries, uses pulverizer
After pulverizing, pack after loading tea bag, obtain Antrodia camphorata fermented tea.
The Antrodia camphorata fermented tea of the preparation method gained according to the present embodiment, containing Antrodia camphorata bacterium in every 100g Antrodia camphorata fermented tea
8.62g, active polysaccharide 4.42g, protein 4.55g, total sugar 9.23g, tea polyphenols 0.86g, aminoacid 0.62g.
Embodiment two
The Antrodia camphorata fermented tea preparation method of the present embodiment comprises the following steps:
Step one, cultivates 12 in malt extract medium under the conditions of temperature is 28 DEG C by ready Antrodia camphorata bacterial strain S-29
My god, obtain the Antrodia camphorata strain activated；
Step 2, is linked into the Antrodia camphorata strain of activation in the liquid seed culture medium after sterilizing, is 28 DEG C of bars in temperature
Under part, cultivate and obtain liquid strain in 4 days；
Step 3, joins 10g glucose and prepares Folium Camelliae sinensis solid medium in 100g black tea after stirring；
Step 4, joins the 100g Folium Camelliae sinensis solid state fermentation culture after at 115 DEG C sterilizing 15 minutes by 70ml tap water
In base, under conditions of temperature is 18 DEG C, soak 4 hours, the Folium Camelliae sinensis solid-state fermentation culture medium after being soaked；
Step 5, is linked into 30ml liquid strain in the Folium Camelliae sinensis solid-state fermentation culture medium after immersion, is 28 DEG C in temperature
Under the conditions of, cultivate 20 days, obtain Antrodia camphorata fermented tea crude product；
Step 6, puts into baking oven by Antrodia camphorata fermented tea crude product, under the conditions of temperature is 20 DEG C, dries, uses pulverizer
After pulverizing, pack after loading tea bag, obtain Antrodia camphorata fermented tea.
The Antrodia camphorata fermented tea of the preparation method gained according to the present embodiment, containing Antrodia camphorata bacterium in every 100g Antrodia camphorata fermented tea
15.77g, active polysaccharide 6.61g, protein 9.79g, total sugar 14.55g, tea polyphenols 2.68g, aminoacid 2.45g.
Embodiment three
The Antrodia camphorata fermented tea preparation method of the present embodiment comprises the following steps:
Step one, cultivates 12 in malt extract medium under the conditions of temperature is 28 DEG C by ready Antrodia camphorata bacterial strain S-29
My god, obtain the Antrodia camphorata strain activated；
Step 2, is linked into the Antrodia camphorata strain of activation in the liquid seed culture medium after sterilizing, is 30 DEG C of bars in temperature
Under part, cultivate and obtain liquid strain in 6 days；
Step 3, prepares Folium Camelliae sinensis solid medium after 15g glucose is joined in 100g black tea and stirred；
Step 4, joins the 100g Folium Camelliae sinensis solid state fermentation culture after at 115 DEG C sterilizing 15 minutes by 100ml tap water
In base (black tea 100g, carbon source is glucose 15g), under conditions of temperature is 22 DEG C, soak 6 hours, the tea after being soaked
Leaf solid-state fermentation culture medium；
Step 5, is linked into 45ml liquid strain in the Folium Camelliae sinensis solid-state fermentation culture medium after immersion, is 30 DEG C in temperature
Under the conditions of, cultivate 25 days, obtain Antrodia camphorata fermented tea crude product；
Step 6, puts into baking oven by Antrodia camphorata fermented tea crude product, under the conditions of temperature is 60 DEG C, dries, uses pulverizer
After pulverizing, pack after loading tea bag, obtain Antrodia camphorata fermented tea.
The Antrodia camphorata fermented tea of the preparation method gained according to the present embodiment, containing Antrodia camphorata bacterium in every 100g Antrodia camphorata fermented tea
14.25g, active polysaccharide 5.89g, protein 6.68g, total sugar 12.51g, tea polyphenols 1.58g, amino acid/11 .35g.
The effect of embodiment and effect
Embodiment one to embodiment three provides the preparation method of a kind of Antrodia camphorata fermented tea, by virtue strong for Antrodia Camphorata bacterium
Fragrance is incorporated in Folium Camelliae sinensis, enriches the odor type of Folium Camelliae sinensis, and the preparation method process of embodiment one to embodiment three is simple, easily-controllable
System, production efficiency is high, easily realizes large-scale production, has important industrial application value, uses embodiment one to enforcement
The Antrodia camphorata fermented tea quality that the preparation method of example three prepares is high, has strong fragranced, compared with traditional zymotic tea, has more
The beneficiating ingredients such as rich in protein, aminoacid and active polysaccharide, are a kind of good health promoting beverage.
Above example is only the basic explanation under present inventive concept, does not limits the invention.And according to the present invention
Any equivalent transformation of being made of technical scheme, belong to protection scope of the present invention.
Claims (9)
1. an Antrodia camphorata fermented tea, it is characterised in that:
Containing Antrodia camphorata bacterium 8.62～15.77g and active polysaccharide 4.42～6.61g in Antrodia camphorata fermented tea described in every 100g.
2. a preparation method for Antrodia camphorata fermented tea, uses Antrodia camphorata bacterial strain (Antrodia camphorata) S-29 to be prepared,
This bacterial strain has been preserved in China General Microbiological preservation center, and deposit number is CGMCC9590, and described preparation method includes following
Step:
Step one, cultivates described Antrodia camphorata bacterial strain S-29 predetermined number of days in malt extract medium, obtains the Antrodia camphorata strain activated；
Step 2, is linked into the Antrodia camphorata strain of described activation in the liquid seed culture medium after sterilizing, is 20 in temperature conditions
～at 30 DEG C, cultivate and obtain liquid strain in 2～6 days；
2～15g carbon sources are joined in 100g Folium Camelliae sinensis and the after fermentation that stirs prepare Folium Camelliae sinensis solid medium by step 3；
Step 4, joins a certain amount of water in described Folium Camelliae sinensis solid-state fermentation culture medium, in the condition that temperature is 15～22 DEG C
Under, soak 2～6 hours, the Folium Camelliae sinensis solid-state fermentation culture medium after being soaked；
Step 5, is linked into described liquid strain in the Folium Camelliae sinensis solid-state fermentation culture medium after described immersion by predetermined ratio,
Under conditions of temperature is 20～30 DEG C, cultivates 12～25 days, obtain Antrodia camphorata fermented tea crude product；
Step 6, puts into baking oven by described Antrodia camphorata fermented tea crude product, under conditions of temperature is 20～60 DEG C, and powder after drying
Broken, pack after loading tea bag, obtain described Antrodia camphorata fermented tea.
The preparation method of Antrodia camphorata fermented tea the most according to claim 2, it is characterised in that:
Wherein, in described step one, described predetermined number of days is 12 days.
The preparation method of Antrodia camphorata fermented tea the most according to claim 2, it is characterised in that:
Wherein, in described step 2, the composition of described liquid seed culture medium is: liquid seed culture medium described in every 1L contains
Glucose 20g, analysis for soybean powder 4g and Semen Maydis starch 2g,
Described liquid seed culture medium sterilizing 20min at 115 DEG C.
The preparation method of Antrodia camphorata fermented tea the most according to claim 2, it is characterised in that:
Wherein, in described step 3, described Folium Camelliae sinensis is any 1 in black tea, green tea, postfermented tea and oolong tea～4 kind.
The preparation method of Antrodia camphorata fermented tea the most according to claim 2, it is characterised in that:
Wherein, in described step 3, described carbon source is any 1～5 in glucose, fructose, maltose, lactose and sucrose
Kind.
The preparation method of Antrodia camphorata fermented tea the most according to claim 2, it is characterised in that:
Wherein, in described step 3, described carbon source is any 1 in rice meal, millet powder, oatmeal, Semen avenae nudae powder, Semen Maydis powder
～5 kinds.
The preparation method of Antrodia camphorata fermented tea the most according to claim 2, it is characterised in that:
Wherein, in described step 4, Folium Camelliae sinensis solid-state fermentation culture medium described in every 100g adds 40～100ml water.
The preparation method of Antrodia camphorata fermented tea the most according to claim 2, it is characterised in that:
Wherein, in described step 5, described predetermined ratio is the Folium Camelliae sinensis solid-state fermentation culture medium after described immersion and described liquid
State strain ratio 100:15～100:45 (g/ml).
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111387311A (en) * | 2020-03-20 | 2020-07-10 | 福建省亚热带植物研究所 | Preparation method of antrodia camphorata oolong tea |
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| CN1227062A (en) * | 1998-02-27 | 1999-09-01 | 福建省农业科学院土壤肥料研究所 | Technology for producing fermented glossy ganoderma tea |
| TW201221065A (en) * | 2011-12-22 | 2012-06-01 | Ting-Chien Liu | Method for producing Antrodia cinnamomea tea leaves |
| CN103125270A (en) * | 2013-02-28 | 2013-06-05 | 深圳市仁泰生物科技有限公司 | High-yield antrodia cinnamomea mycelium fermentation method for triterpenoids |
| CN104222371A (en) * | 2013-06-19 | 2014-12-24 | 张平 | Formula and production process of antrodia camphorata fermented tea |
| TW201536185A (en) * | 2014-03-21 | 2015-10-01 | ming-cong Li | Fabrication method for antrodia cinnamomea tea and antrodia cinnamomea tea fabricated therewith |
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2016
- 2016-06-22 CN CN201610454186.7A patent/CN106106884A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1227062A (en) * | 1998-02-27 | 1999-09-01 | 福建省农业科学院土壤肥料研究所 | Technology for producing fermented glossy ganoderma tea |
| TW201221065A (en) * | 2011-12-22 | 2012-06-01 | Ting-Chien Liu | Method for producing Antrodia cinnamomea tea leaves |
| CN103125270A (en) * | 2013-02-28 | 2013-06-05 | 深圳市仁泰生物科技有限公司 | High-yield antrodia cinnamomea mycelium fermentation method for triterpenoids |
| CN104222371A (en) * | 2013-06-19 | 2014-12-24 | 张平 | Formula and production process of antrodia camphorata fermented tea |
| TW201536185A (en) * | 2014-03-21 | 2015-10-01 | ming-cong Li | Fabrication method for antrodia cinnamomea tea and antrodia cinnamomea tea fabricated therewith |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111387311A (en) * | 2020-03-20 | 2020-07-10 | 福建省亚热带植物研究所 | Preparation method of antrodia camphorata oolong tea |
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Application publication date: 20161116 |