CN106106185A - A kind of Cyclocarya paliurus Iljinskaja rooting of vitro seedling culture medium and root culture method - Google Patents
A kind of Cyclocarya paliurus Iljinskaja rooting of vitro seedling culture medium and root culture method Download PDFInfo
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- CN106106185A CN106106185A CN201610691975.2A CN201610691975A CN106106185A CN 106106185 A CN106106185 A CN 106106185A CN 201610691975 A CN201610691975 A CN 201610691975A CN 106106185 A CN106106185 A CN 106106185A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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Abstract
The invention discloses a kind of Cyclocarya paliurus Iljinskaja rooting of vitro seedling culture medium and root culture method.The formula of described root media is: improvement MS+IBA0.5～2.0mg/L+IAA0～2.0mg/L+ diethyl aminoethyl hexanoate 0.02～0.1mg/L+ sucrose 15～20g/L, pH5.5～6.0；Wherein, the formula of improvement MS is: a great number of elements: potassium nitrate 700mg/L, ammonium nitrate 600mg/L, Magnesium sulfate heptahydrate 185mg/L, potassium dihydrogen phosphate 60mg/L, calcium chloride dihydrate 165mg/L；Organic compound: inositol 100mg/L, nicotinic acid 1mg/L, pyridoxine hydrochloride 1mg/L, thiamine hydrochloride 0.2mg/L, glycine 2mg/L；Trace element is with MS culture medium.By above-mentioned culture medium, Cyclocarya paliurus Iljinskaja test tube Seedling is carried out root culture, rooting rate >=70%.
Description
Technical field
The present invention relates to the tissue culture technique of plant, be specifically related to a kind of Cyclocarya paliurus Iljinskaja rooting of vitro seedling culture medium and take root
Cultural method.
Background technology
It is blue or green that Cyclocarya paliurus Iljinskaja [Cyclocarya paliurus (Batal.) Iljinskaja] belongs to Juglandaceae (Juglandaceae)
Money sallow, another name money tree, fiber crops willow, blue or green money Lee, Boehmeria longispica Steud. willow, Shan Huashu.Cyclocarya paliurus Iljinskaja is described as " plant giant panda ", " doctor
3rd tree of educational circles ", it is the rare tree species that survives of glacier four discipline, exists only in China, be the in imminent danger of special-protection-by-the-State
Plant.Cyclocarya paliurus Iljinskaja is distributed mainly on the ground such as Jiangxi, Zhejiang, Jiangsu, Anhui, Fujian, Taiwan, Hubei, Sichuan, Guizhou, Yunnan, many
Sporadicly it is distributed in remote, thickly forested mountains and some nature reserve areas.
Cyclocarya paliurus Iljinskaja has more health care and the medical value of uniqueness.Among the people do tea with leaf of Cyclocarya paliurus Iljinskaja for a long time, tea gas delicate fragrance,
Taste is sweet." Chinese medicine resource will want " (1994) are recorded, and leaf of Cyclocarya paliurus Iljinskaja tool heat clearing away is quenched one's thirst the effect of removing toxic substances." whole nation Chinese herbal medicine name mirror "
(1996) recording, Cyclocarya paliurus Iljinskaja bark, leaf, root have effect that killing parasites for relieving itching, anti-inflammatory analgetic dispel the wind.Modern pharmacology research proves,
Cyclocarya paliurus Iljinskaja has lowering blood pressure and blood fat and anti-ageing medicinal efficacy of waiting for a long time.Research find, leaf of Cyclocarya paliurus Iljinskaja contain substantial amounts of potassium, calcium, magnesium,
The macroelements such as phosphorus, possibly together with trace element such as manganese, ferrum, copper, chromium, zinc, selenium, vanadium, germanium.Wherein, make with carbohydrate metabolism and insulin
With closely-related elemental chromium, nickel, lithium, selenium, vanadium content higher, it is possible to assist insulin plays hypoglycemic activity, and can change
Kind carbohydrate tolerance.Leaf of Cyclocarya paliurus Iljinskaja possibly together with organic compound such as abundant polysaccharide, flavone, triterpene, steroid, saponin, coumarins, this
A little compounds can effectively reduce triglyceride and cholesterol, makes Cyclocarya paliurus Iljinskaja have the function of blood pressure lowering, blood sugar lowering, blood fat reducing, with
Time to strengthening body immunity, antioxidation, anti-ageing waiting for a long time have unique effect.
At present, the Cyclocarya paliurus Iljinskaja nursery stock on market mostly is wild resource transplanting or the seedling from seed of artificial culture.Due to green grass or young crops
Money willow kind source is limited, and the full rate of seed is low, also has deep dormancy characteristic, uses conventional seed planting method for culturing seedlings breeding cycle long, effect
Rate is low, it is difficult to produce a large amount of seedling in a short time, it is impossible to meet the demand in market.In recent years, domestic and international research worker with
In vitro Embryo, blade, tender shoots, stem section etc. in succession carry out the research of the tissue culture and rapid proliferation of Cyclocarya paliurus Iljinskaja for outer implant, as thanked
The third of the twelve Earthly Branches peak etc. (Xie Yinfeng etc. the preliminary study of Cyclocarya paliurus Iljinskaja tissue-culturing rapid propagation system. northwest Botany Gazette, 2009,29 (11): 2331-
2338) utilize In vitro Embryo tentatively to establish the rapid propagation system of Cyclocarya paliurus Iljinskaja, use WPM+0.2mg/L IBA+20g/L sucrose medium
Inducing, rooting rate is 16.67%, processes through 15d secretly induction, and rooting rate can bring up to 23.33%.Qiao Qingmei etc. are (tall
Minister in ancient times prunus mume (sieb.) sieb.et zucc., Cheng Maogao, Wei Zhihua. the impact on Cyclocarya paliurus Iljinskaja Jing Duan tissue culture of the different exogenous hormones. north gardening 2009 (7):
125-127) research finds, in Cyclocarya paliurus Iljinskaja Jing Duan tissue culture, the appropriate media of root induction is improvement MS+5.0mg/
L IBA, inductivity is 40.00%.Lu Meng etc. (Lu Meng etc. the Techniques of in Vitro Culture of Cyclocarya paliurus Iljinskaja stem segment with axillary buds. Nanjing Forestry University
Journal (natural science edition), 2013,37 (6): 6-10) with WPM as minimal medium, research IBA, GGR-6, ABT-1 are to blue or green money
The impact of Liu Shenggen, result shows, 10%GGR+1.0mg/L IBA combined treatment effect is best, and rooting rate is 25%.Existing
Result of study shows, Cyclocarya paliurus Iljinskaja test tube Seedling can success expanding propagation, but its rooting rate is low, quality of rooting is poor, it is difficult to meet batch production
The requirement of nursery.As can be seen here, the rooting technique of Cyclocarya paliurus Iljinskaja tissue cultured seedling has become the pass of restriction Cyclocarya paliurus Iljinskaja seedling factorial praluction
Key link, the breakthrough of tissue cultured seedling rooting technique, to set up Cyclocarya paliurus Iljinskaja efficient tissue-culturing rapid propagation system, expand planting scale have important
Meaning.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of Cyclocarya paliurus Iljinskaja rooting of vitro seedling culture medium and root culture method.
Use root media of the present invention that conventional Cyclocarya paliurus Iljinskaja test tube Seedling is carried out root culture, it is possible to obtain the life of more than 70%
Root rate, and root system development is good, plant strain growth is healthy and strong, and transplanting survival rate is high.
The formula of Cyclocarya paliurus Iljinskaja rooting of vitro seedling culture medium of the present invention is:
Improvement MS+IBA0.5～2.0mg/L+IAA0～2.0mg/L+ diethyl aminoethyl hexanoate 0.02～0.1mg/L+ sucrose 15～20g/
L, pH5.5～6.0；
Wherein, the formula of improvement MS is:
The component of a great number of elements and concentration is: potassium nitrate 700mg/L, ammonium nitrate 600mg/L, Magnesium sulfate heptahydrate 185mg/L,
Potassium dihydrogen phosphate 60mg/L, calcium chloride dihydrate 165mg/L；
The component of organic compound and concentration is: inositol 100mg/L, nicotinic acid 1mg/L, pyridoxine hydrochloride 1mg/L, hydrochloric acid sulfur
Amine element 0.2mg/L, glycine 2mg/L；
The component of trace element and concentration are with MS culture medium.
In technique scheme, the formula of described Cyclocarya paliurus Iljinskaja root media is preferably: improvement MS+IBA0.5～1.0mg/
L+IAA 0.8～1.5mg/L+ diethyl aminoethyl hexanoate 0.05～0.08mg/L+ sucrose 18～20g/L, pH5.5～6.0；Most preferably improvement
MS+IBA 0.5mg/L+IAA 1.0mg/L+ diethyl aminoethyl hexanoate 0.05mg/L+ sucrose 20g/L, pH5.5～6.0.Described Cyclocarya paliurus Iljinskaja is taken root
The pH value of culture medium is preferably 5.8～6.0.
Present invention additionally comprises the root culture method of Cyclocarya paliurus Iljinskaja test tube Seedling, particularly as follows: cut individual plant test tube Seedling, access is taken root
Culture medium is cultivated at least 25d；Wherein:
The formula of described root media is:
Improvement MS+IBA0.5～2.0mg/L+IAA0～2.0mg/L+ diethyl aminoethyl hexanoate 0.02～0.1mg/L+ sucrose 15～20g/
L, pH5.5～6.0；
Wherein, the formula of improvement MS is:
The component of a great number of elements and concentration is: potassium nitrate 700mg/L, ammonium nitrate 600mg/L, Magnesium sulfate heptahydrate 185mg/L,
Potassium dihydrogen phosphate 60mg/L, calcium chloride dihydrate 165mg/L；
The component of organic compound and concentration is: inositol 100mg/L, nicotinic acid 1mg/L, pyridoxine hydrochloride 1mg/L, hydrochloric acid sulfur
Amine element 0.2mg/L, glycine 2mg/L；
The component of trace element and concentration are with MS culture medium.
In said method, the root culture condition of Cyclocarya paliurus Iljinskaja test tube Seedling can be same as the prior art.It is preferably as follows: cultivate
Temperature is 20～30 DEG C, and periodicity of illumination is 10～14h/d, and intensity of illumination is 2000～3000Lx；Further preferred cultivation bar
Part is: cultivation temperature is 23～27 DEG C, and periodicity of illumination is 10～12h/d, and intensity of illumination is 2000～3000Lx.
In said method, when Cyclocarya paliurus Iljinskaja test tube Seedling cultivates 25d in root media under above-mentioned condition of culture, rooting rate
Can reach more than 70%, and root system development is good, plant strain growth is healthy and strong.In the method for the invention, preferably test tube Seedling is being given birth to
Incubation time in root culture medium is 25～35d.
In said method, the formula of described Cyclocarya paliurus Iljinskaja root media is preferably: improvement MS+IBA0.5～1.0mg/L+
IAA 0.8～1.5mg/L+ diethyl aminoethyl hexanoate 0.05～0.08mg/L+ sucrose 18～20g/L, pH5.5～6.0；Most preferably improve MS
+ IBA 0.5mg/L+IAA 1.0mg/L+ diethyl aminoethyl hexanoate 0.05mg/L+ sucrose 20g/L, pH5.5～6.0.Described Cyclocarya paliurus Iljinskaja is taken root training
The pH value supporting base is preferably 5.8～6.0.
In the method for the invention, obtain by existing conventional method isolated culture for carrying out the test tube Seedling of root culture,
Preferably with Cyclocarya paliurus Iljinskaja current-year branch, isolated culture gained according to a conventional method.Preferably choose the individual plant test tube of height >=2cm
Seedling carries out root culture, more preferably chooses height >=3cm and length has the individual plant test tube Seedling of 3～4 compound leaves to carry out root culture,
So can improve the transplanting survival rate of plant further.
Compared with prior art, present invention is characterized in that by improvement minimal medium concentration of component, be used in combination spy
Determine the diethyl aminoethyl hexanoate of concentration, IBA and IAA plant growth regulator, make gained root media can effectively facilitate Cyclocarya paliurus Iljinskaja test tube
Taking root of Seedling.Utilize this root media that Cyclocarya paliurus Iljinskaja test tube Seedling is carried out root culture, rooting rate can be made to reach more than 70% (excellent
Select embodiment up to 86%), and root system development is good, plant strain growth is healthy and strong, and transplanting survival rate is high, produces for realizing the training of Cyclocarya paliurus Iljinskaja group
Industry and popularization and application provide technical support.
Accompanying drawing explanation
Fig. 1 is the picture of the Cyclocarya paliurus Iljinskaja test tube Seedling obtained according to a conventional method in embodiment 1；
The picture of the Seedling of taking root when Fig. 2 is to use the root media cultivation 25d described in the embodiment of the present invention 1.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in further detail, to be more fully understood that present disclosure, but
The present invention is not limited to following example.
In following embodiment, involved improvement MS includes a great number of elements, trace element and organic compound, concrete
Formula is as follows:
The component of a great number of elements and concentration is: potassium nitrate 700mg/L, ammonium nitrate 600mg/L, Magnesium sulfate heptahydrate 185mg/L,
Potassium dihydrogen phosphate 60mg/L, calcium chloride dihydrate 165mg/L；
The component of trace element and concentration is: potassium iodide 0.83mg/L, boric acid 6.2mg/L, four water manganese sulfate 22.3mg/L,
Zinc sulphate heptahydrate 8.6mg/L, Sodium Molybdate Dihydrate 0.25mg/L, copper sulphate pentahydrate 0.025mg/L, cobalt chloride hexahydrate 0.025mg/L,
Ferrous sulfate heptahydrate 27.8mg/L.Wherein, ferrous sulfate heptahydrate chelates with disodiumedetate, disodiumedetate
Concentration is 37.3mg/L.
The component of organic compound and concentration is: inositol 100mg/L, nicotinic acid 1mg/L, pyridoxine hydrochloride 1mg/L, hydrochloric acid sulfur
Amine element 0.2mg/L, glycine 2mg/L.
Embodiment 1
1) taking Cyclocarya paliurus Iljinskaja current-year branch, isolated culture obtains test tube Seedling according to a conventional method, as shown in Figure 1；
2) root culture: cut height more than 3.5cm and the long individual plant test tube Seedling having 3～4 compound leaves, access root culture
Base is cultivated 25d (when cultivating 25d as shown in Figure 2)；Wherein:
The formula of described root media is: improvement MS+IBA0.5mg/L+IAA1.0mg/L+ diethyl aminoethyl hexanoate 0.05mg/L+ sugarcane
Sugar 20g/L, pH5.8, using filter paper bridge is supporter.
Root culture condition is: temperature is 23～25 DEG C, and periodicity of illumination is 12h/d, and intensity of illumination is 2000Lx.
By strain number of taking root/inoculation strain number × 100% to calculate rooting rate (following identical), rooting rate is 86%.
Comparative example 1
The method repeating embodiment 1, is simply revised as prescription of rooting medium: improvement MS+IBA0.5mg/L+IAA
1.0mg/L+ sucrose 20g/L, pH5.8.
Being computed, rooting rate is 25%.
Comparative example 2
The method repeating embodiment 1, is simply revised as prescription of rooting medium: improvement MS+IBA 0.5mg/L+IAA
1.0mg/L+ diethyl aminoethyl hexanoate 0.01mg/L+ sucrose 20g/L, pH5.8.
Being computed, rooting rate is 40%.
Comparative example 3
The method repeating embodiment 1, is simply revised as prescription of rooting medium: improvement MS+IBA0.5mg/L+IAA
1.0mg/L+ diethyl aminoethyl hexanoate 0.15mg/L+ sucrose 20g/L, pH5.8.
Being computed, rooting rate is 5%.
Embodiment 2
1) taking Cyclocarya paliurus Iljinskaja current-year branch, isolated culture obtains test tube Seedling according to a conventional method；
2) root culture: cut higher than 3.5cm and the long individual plant test tube Seedling having 3～4 compound leaves, access in root media
Cultivate 25d；Wherein:
The formula of described root media is: improvement MS+IBA1.5mg/L+ diethyl aminoethyl hexanoate 0.05mg/L+ sucrose 20g/L,
PH5.8, using filter paper bridge is supporter.
Condition of culture in root media is: temperature is 23～25 DEG C, and periodicity of illumination is 12h/d, and intensity of illumination is
2000Lx。
Being computed, rooting rate is 80%.
Embodiment 3
1) taking Cyclocarya paliurus Iljinskaja current-year branch, isolated culture obtains test tube Seedling according to a conventional method；
2) root culture: cut higher than 2.5cm and the long individual plant test tube Seedling having 2～3 compound leaves, access in root media
Cultivate 30d；Wherein:
The formula of described root media is: improvement MS+IBA0.5mg/L+IAA 2.0mg/L+ diethyl aminoethyl hexanoate 0.02mg/L+
Sucrose 15g/L, pH6.0, using filter paper bridge is supporter.
Condition of culture in root media is: temperature is 25 DEG C, and periodicity of illumination is 10h/d, and intensity of illumination is
3000Lx。
Being computed, rooting rate is 75%.
Embodiment 4
1) taking Cyclocarya paliurus Iljinskaja current-year branch, isolated culture obtains test tube Seedling according to a conventional method；
2) root culture: cut higher than 2.5cm and the long individual plant test tube Seedling having 1～2 compound leaf, access in root media
Cultivate 27d；Wherein:
The formula of described root media is: improvement MS+IBA1.5mg/L+IAA0.5mg/L+ diethyl aminoethyl hexanoate 0.1mg/L+ sugarcane
Sugar 18g/L, pH5.5, using filter paper bridge is supporter.
Condition of culture in root media is: temperature is 20 DEG C, and periodicity of illumination is 14h/d, and intensity of illumination is
2500Lx。
Being computed, rooting rate is 70%.
Embodiment 5
1) taking Cyclocarya paliurus Iljinskaja current-year branch, isolated culture obtains test tube Seedling according to a conventional method；
2) root culture: choose higher than 3.5cm and the long individual plant test tube Seedling having 3～4 compound leaves, access in root media
Cultivate 28d；Wherein:
The formula of described root media is: improvement MS+IBA1.0mg/L+IAA1.5mg/L+ diethyl aminoethyl hexanoate 0.08mg/L+ sugarcane
Sugar 16g/L, pH5.9, using filter paper bridge is supporter.
Condition of culture in root media is: temperature is 30 DEG C, and periodicity of illumination is 12h/d, and intensity of illumination is 2000
～2200Lx.
Being computed, rooting rate is 82%.
Claims (7)
1. a Cyclocarya paliurus Iljinskaja rooting of vitro seedling culture medium, it is characterised in that: the formula of this root media is:
Improvement MS+IBA0.5～2.0mg/L+IAA0～2.0mg/L+ diethyl aminoethyl hexanoate 0.02～0.1mg/L+ sucrose 15～20g/L,
PH5.5～6.0；
Wherein, the formula of improvement MS is:
The component of a great number of elements and concentration is: potassium nitrate 700mg/L, ammonium nitrate 600mg/L, Magnesium sulfate heptahydrate 185mg/L, phosphoric acid
Potassium dihydrogen 60mg/L, calcium chloride dihydrate 165mg/L；
The component of organic compound and concentration is: inositol 100mg/L, nicotinic acid 1mg/L, pyridoxine hydrochloride 1mg/L, thiamine hydrochloride
0.2mg/L, glycine 2mg/L；
The component of trace element and concentration are with MS culture medium.
Cyclocarya paliurus Iljinskaja rooting of vitro seedling culture medium the most according to claim 1, it is characterised in that: joining of described root media
Fang Wei:
Improvement MS+IBA0.5mg/L+IAA1.0mg/L+ diethyl aminoethyl hexanoate 0.05mg/L+ sucrose 20g/L, pH5.5～6.0.
3. the root culture method of a Cyclocarya paliurus Iljinskaja test tube Seedling, it is characterised in that: cut individual plant test tube Seedling, access root media
Middle cultivation at least 25d；Wherein:
The formula of described root media is:
Improvement MS+IBA0.5～2.0mg/L+IAA0～2.0mg/L+ diethyl aminoethyl hexanoate 0.02～0.1mg/L+ sucrose 15～20g/L,
PH5.5～6.0；
Wherein, the formula of improvement MS is:
The component of a great number of elements and concentration is: potassium nitrate 700mg/L, ammonium nitrate 600mg/L, Magnesium sulfate heptahydrate 185mg/L, phosphoric acid
Potassium dihydrogen 60mg/L, calcium chloride dihydrate 165mg/L；
The component of organic compound and concentration is: inositol 100mg/L, nicotinic acid 1mg/L, pyridoxine hydrochloride 1mg/L, thiamine hydrochloride
0.2mg/L, glycine 2mg/L；
The component of trace element and concentration are with MS culture medium.
The root culture method of Cyclocarya paliurus Iljinskaja test tube Seedling the most according to claim 3, it is characterised in that: the training of taking root of test tube Seedling
Foster condition is: cultivation temperature is 20～30 DEG C, and periodicity of illumination is 10～14h/d, and intensity of illumination is 2000～3000Lx.
The root culture method of Cyclocarya paliurus Iljinskaja test tube Seedling the most according to claim 4, it is characterised in that: the training of taking root of test tube Seedling
Foster condition is: cultivation temperature is 23～27 DEG C, and periodicity of illumination is 10～12h/d, and intensity of illumination is 2000～3000Lx.
6. according to the root culture method of the Cyclocarya paliurus Iljinskaja test tube Seedling according to any one of claim 3～5, it is characterised in that: examination
Pipe seedling rooting incubation time is 25～35d.
7. according to the root culture method of the Cyclocarya paliurus Iljinskaja test tube Seedling according to any one of claim 3～5, it is characterised in that: institute
The formula stating root media is:
Improvement MS+IBA0.5mg/L+IAA1.0mg/L+ diethyl aminoethyl hexanoate 0.05mg/L+ sucrose 20g/L, pH5.5～6.0.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107372115A (en) * | 2017-08-14 | 2017-11-24 | 湖北瑞晟生物有限责任公司 | The culture medium and its cultural method of a kind of plant with difficult rooting |
| CN113604498A (en) * | 2021-08-02 | 2021-11-05 | 华南农业大学 | Stylosanthes guianensis hairy root induction method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102487819A (en) * | 2011-11-22 | 2012-06-13 | 嘉兴浙华农业技术开发有限公司 | Cyclocarya paliurus callus and suspension culture method for callus particles |
| CN105052745A (en) * | 2015-08-24 | 2015-11-18 | 华南农业大学 | Method for promoting growth of echinacea purpurea regeneration buds |
-
2016
- 2016-08-17 CN CN201610691975.2A patent/CN106106185B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102487819A (en) * | 2011-11-22 | 2012-06-13 | 嘉兴浙华农业技术开发有限公司 | Cyclocarya paliurus callus and suspension culture method for callus particles |
| CN105052745A (en) * | 2015-08-24 | 2015-11-18 | 华南农业大学 | Method for promoting growth of echinacea purpurea regeneration buds |
Non-Patent Citations (5)
| Title |
|---|
| RUILING YAO ETAL.: "Calcium alleviation of sodium toxicity in salt-treated Cyclocarya paliurus seedlings", 《AFRICAN JOURNAL OF AGRICULTURAL RESEARCH》 * |
| 胡海涛等: "余甘子下胚轴离体再生体系的优化", 《园艺学报》 * |
| 谢寅峰等: "青钱柳子叶不定根的发生机制", 《林业科学》 * |
| 鲁萌等: "青钱柳茎段腋芽的离体培养技术", 《南京林业大学学报( 自然科学版)》 * |
| 龚峥等: "枫香组织培养快繁育苗技术研究", 《广东林业科技》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107372115A (en) * | 2017-08-14 | 2017-11-24 | 湖北瑞晟生物有限责任公司 | The culture medium and its cultural method of a kind of plant with difficult rooting |
| CN113604498A (en) * | 2021-08-02 | 2021-11-05 | 华南农业大学 | Stylosanthes guianensis hairy root induction method |
| CN113604498B (en) * | 2021-08-02 | 2024-04-12 | 华南农业大学 | Method for inducing hairy roots of stylosanthes guianensis |
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