CN106093320A - A kind of method for quick of tea bee pollen freshness - Google Patents
A kind of method for quick of tea bee pollen freshness Download PDFInfo
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- CN106093320A CN106093320A CN201610452690.3A CN201610452690A CN106093320A CN 106093320 A CN106093320 A CN 106093320A CN 201610452690 A CN201610452690 A CN 201610452690A CN 106093320 A CN106093320 A CN 106093320A
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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Abstract
The method for quick that a kind of tea bee pollen freshness is provided of the present invention, by lyophilization tea to be measured bee pollen, breaking cellular wall, sieve, a, b value of tea bee pollen powder sample to be measured is measured with colour difference meter, tea bee pollen powder sample a value and the freshness of b value judgement testing sample is recorded: if a value is below 13 with colour difference meter, b value is below 50, i.e. can determine that this tea bee pollen is to be equivalent to 35 DEG C of high temperature above storages more than 3 months, regarding as stale.The present invention accurately measures mechanical breaking-wall method tea bee pollen a value and b value after drying by color difference meter, and then judges tea bee pollen freshness according to this index.The inventive method is reasonable in design, has easy and simple to handle, the shortest, can complete in several minutes；Highly sensitive, reproducible, it is easy to standardization；Equipment requirements is the highest, extremely easily penetration and promotion.Can apply in quickly detection tea bee pollen freshness or other bee pollen freshness.
Description
Technical field
The invention belongs to bee product detection field, relate to the detection method of a kind of bee product freshness, particularly relate to one
The method for quick of tea bee pollen freshness, is exactly a kind of side using chromatism method to judge tea bee pollen freshness
Method.
Background technology
Bee pollen is the flower that Apis microsporangium from the male flower pesticide of angiosperm and gymnosperm microsporophyll gathers
Powder, through the pollen dough that Apis processes.As a kind of natural resources, bee pollen does not contain only very abundant
Protein, fat, saccharide, trace element, vitamin, have the nucleic acid of special efficacy, Huang possibly together with to organism physiological function
The bioactive substance such as ketone, hormone, is described as " multipotential nutrition storehouse ", " complete nutrition product ".Deep along with what bee pollen was studied
Entering, its multiple physiological and pharmacological function having progressively is excavated, such as pre-anti-aging, skin maintenance, enhancing immunity, anti-hypoxia
Deng, prostatosis, cardiovascular and cerebrovascular disease, hepatopathy, habitual constipation, anemia, diabetes etc. are had certain treatment and auxiliary simultaneously
Treatment function, is the most also described as " the natural Drug Storage of concentration ".
But, along with prolongation and the change of storage requirement of bee pollen period of storage, the nutritional labeling in bee pollen and
Bioactive substance can gradually decrease, and correspondingly, the health-care effect that bee pollen is had also can decrease and even disappear.Research
Find, after bee pollen at room temperature preserves 1 year, the oxidation resistance more than 50% can be lost.Therefore, bee pollen freshness with
Close relationship is there is between its quality.
As far back as eighties of last century, external researcher begins to carry out the relation between bee pollen component and storage requirement
Research, and some indexs that can quantify in bee pollen storage process are studied.But the composition due to bee pollen
Complexity, the component difference of different plant origin pollen is bigger, and the most sensitive to storage condition, finds and establishes bee pollen
The appropriate criteria not a duck soup of freshness.Up to now, in the world neither one generally acknowledge can reflect bee pollen freshness strictly according to the facts
And the index that can promote in actual applications, there is no a kind of applicable evaluation methodology yet.
The existing Apis of China more than 900 ten thousand groups, produces bee pollen about 4500 tons (not including that beekeeper is from being left feedstuff part) per year,
Domestic processing and retail sales are about 2500 tons, export about 2000 tons.And in the upstream of this great market demand, gather,
In the links such as storage and transport, famine keeps the consciousness solar exposure of its freshness, high temperature to dry, non-refrigerated
The operations such as transport all can cause bee pollen freshness drastically to decline, and then makes the quality processed raw material be greatly reduced.
Therefore, finding suitable bee pollen freshness index and corresponding detection method is extremely the most urgent, this is not only
Contributing to specification bee pollen market, be also the expansion to bee pollen scientific research field with deeply, the development to bee pollen industry has
Great strategic importance.
Summary of the invention
It is an object of the invention to provide the method for quick of a kind of tea bee pollen freshness.The method is by color difference meter
Accurately measure mechanical breaking-wall method tea bee pollen a value and b value after drying, and then judge tea bee pollen freshness according to this index.
The inventive method is realized by following steps:
(1) by after freeze-dried for tea bee pollen to be measured, carry out mechanical breaking-wall method, cross the sieve of 100 mesh, weigh more than 2 grams
Sample is laid in planchet mensuration；
(2) measure a, b value of tea bee pollen powder sample, independent mensuration 3 times with colour difference meter, average；
(3) tea bee pollen powder sample a value and the freshness of b value judgement testing sample are recorded with colour difference meter: if a value is less than
13, and b value is less than 50, i.e. can determine that this tea bee pollen is to be equivalent to 35 DEG C of high temperature above storages more than 3 months, regarding as not
Fresh；If a and b value respectively higher than 13 and 50, it is taken as fresh.
Solid and liquid color are quantified by domestic and international commonly used spectrophotometric color measurement instrument, represent face with Lab colorimeter system
Chromatic number evidence, Lab system be International Commission on Illumination issue a kind of even color system, i.e. between color visual distance with
Euclidean distance on color coordinates is directly proportional, and this is presently the most one of conventional colorimeter system.Within the system, have one
Individual lightness factor L and two colourity factor a and b:L value are between 0～100, and the biggest expression of L-value is the brightest, otherwise the darkest；A, b value
Between-120～120 ,+a represents red, and-a represents green, and+b represents yellow, and-b represents blue.The color of any color becomes
Change can represent with a, b numerical value, and the layer variability of any color can represent with L numerical value.The present invention uses a, b numerical value pair
The color of the tea bee pollen under different conditions of storage quantifies, thus judges the freshness of tea bee pollen.
It is a further object to provide described method to apply in the quickly detection of tea bee pollen freshness.
The present invention is made by color difference meter and accurately measures mechanical breaking-wall method tea bee pollen a value and b value after drying, and then according to this
Indexs measure tea bee pollen freshness.The inventive method is reasonable in design, has the following characteristics that (1) is easy and simple to handle, the shortest,
Can complete in several minutes.(2) highly sensitive, reproducible, it is easy to standardization.(3) equipment requirements is the highest, the most easily popularizes
Promote.
Accompanying drawing explanation
Fig. 1 is that the color that at 35 DEG C, different periods of storage process tea bee pollen compares.
Fig. 2 is that the color that under room temperature, different periods of storage process tea bee pollen compares.
Detailed description of the invention
The present invention is further described in conjunction with the accompanying drawings and embodiments.
Embodiment 1
Store 1 month, 2 months, 3 months, 6 months and 12 months at tea bee pollen 35 DEG C, carry out mechanical breaking-wall method, cross 100
Purpose sieve, weighs and measures in planchet more than 2 grams of sample tilings, measure a, b of tea bee pollen powder sample with colour difference meter
Value, independent mensuration 3 times, average.
Weigh the bee pollen sample to be measured that about 0.3g has pulverized, add 7.5mL EtOH Sonicate assisted extraction, extract completely
After, cross 0.22 μm filter membrane, it is thus achieved that filtrate, and with to be measured after the corresponding dilution of ethanol work.Precision weighs DPPH standard substance about 15mg,
Be settled to 15mL with ethanol, obtain DPPH standard working solution (1mg/mL), low temperature (4 DEG C), keep in Dark Place standby.With vitamin C
For reference substance, at microplate reader wavelength 517nm, measure absorbance so that it is reading is between 0.2～0.8.
DPPH clearance rate (%)=(A0-AS)/A0 × 100%
In formula: AS: add the absorbance of the DPPH solution of sample extracting solution；
A0: add the absorbance of the DPPH solution of dehydrated alcohol.
Calculate and remove sample and the concentration of vitamin C reference substance, i.e. 503nhibiting concentration IC50, comparison specimen needed for 50%DPPH
Product free radical scavenging.
A, b value and the antioxidant activity of different time is stored at table 1 tea bee pollen 35 DEG C
| Time (moon) | 1 | 2 | 3 | 6 | 12 |
| a | 21.3 | 16.4 | 12.5 | 11.3 | 9.6 |
| b | 66.7 | 57.9 | 48.6 | 45.2 | 40.5 |
| IC50 (mg dry/mL) | 3.2 | 3.5 | 4.1 | 4.7 | 5.8 |
As seen from Table 1, when tea bee pollen stores 3 months at 35 DEG C, a, b value is remarkably decreased, and IC50 value is significantly raised.Knot
Closing Fig. 1 color reaction visible, when Flos Camelliae Japonicae powder stores more than 2 months, color and luster substantially shoals, therefore, and can be according to tea bee pollen 35
DEG C storage 3 months time data as comparison, even a value is below 13, or b value is below 50, i.e. can determine that tea bee pollen is
Be equivalent to store more than 3 months 35 DEG C of high temperature above, regard as stale.
Embodiment 2
Tea bee pollen storage in room temperature 1 month, 2 months, 3 months, 6 months and 12 months, carries out mechanical breaking-wall method, crosses 100
Purpose sieve, weighs and measures in planchet more than 2 grams of sample tilings, measure a, b of tea bee pollen powder sample with colour difference meter
Value, independent mensuration 3 times, average.
Weigh the bee pollen sample to be measured that about 0.3g has pulverized, add 7.5mL EtOH Sonicate assisted extraction, extract completely
After, cross 0.22 μm filter membrane, it is thus achieved that filtrate, and with to be measured after the corresponding dilution of ethanol work.Precision weighs DPPH standard substance about 15mg,
Be settled to 15mL with ethanol, obtain DPPH standard working solution (1mg/mL), low temperature (4 DEG C), keep in Dark Place standby.With vitamin C
For reference substance, at microplate reader wavelength 517nm, measure absorbance so that it is reading is between 0.2～0.8.
DPPH clearance rate (%)=(A0-AS)/A0 × 100%
In formula: AS: add the absorbance of the DPPH solution of sample extracting solution；
A0: add the absorbance of the DPPH solution of dehydrated alcohol.
Calculate and remove sample and the concentration of vitamin C reference substance, i.e. 503nhibiting concentration IC50, comparison specimen needed for 50%DPPH
Product free radical scavenging.
A, b value of table 2 tea bee pollen room temperature for storage different time and antioxidant activity
| Time (moon) | 1 | 2 | 3 | 6 | 12 |
| a | 23.8 | 19.9 | 17.6 | 15.7 | 10.9 |
| b | 72.2 | 60.5 | 56.7 | 52.6 | 43.3 |
| IC50 (mg dry/mL) | 3.0 | 3.2 | 3.5 | 3.8 | 4.5 |
As seen from Table 2, when tea bee pollen at room temperature stores 12 months, a, b value is remarkably decreased, and color and luster substantially shoals,
IC50 value is significantly raised, can according to tea bee pollen 35 DEG C storage 3 months time data as comparison, even a value below 13,
B value, below 50, i.e. can determine that tea bee pollen is the equal of to store more than 3 months 35 DEG C of high temperature above, regards as stale.
Visible in conjunction with table 2 and Fig. 2, Flos Camelliae Japonicae powder store 6 months time, color and luster shoals, a, b value decline, be equivalent to 35 DEG C storages 2 months time
Color.
Embodiment 3
Store 1 month, 2 months, 3 months, 6 months and 12 months at tea bee pollen 4 DEG C, carry out mechanical breaking-wall method, cross 100 mesh
Sieve, weighing and measuring in planchet more than the tiling of 2 grams of samples, measuring a, b value of tea bee pollen powder sample with colour difference meter,
Independent mensuration 3 times, averages.
Weigh the bee pollen sample to be measured that about 0.3g has pulverized, add 7.5mL EtOH Sonicate assisted extraction, extract completely
After, cross 0.22 μm filter membrane, it is thus achieved that filtrate, and with to be measured after the corresponding dilution of ethanol work.Precision weighs DPPH standard substance about 15mg,
Be settled to 15mL with ethanol, obtain DPPH standard working solution (1mg/mL), low temperature (4 DEG C), keep in Dark Place standby.With vitamin C
For reference substance, at microplate reader wavelength 517nm, measure absorbance so that it is reading is between 0.2～0.8.
DPPH clearance rate (%)=(A0-AS)/A0 × 100%
In formula: AS: add the absorbance of the DPPH solution of sample extracting solution；
A0: add the absorbance of the DPPH solution of dehydrated alcohol.
Calculate and remove sample and the concentration of vitamin C reference substance, i.e. 503nhibiting concentration IC50, comparison specimen needed for 50%DPPH
Product free radical scavenging.
A, b value and the antioxidant activity of different time is stored at table 3 tea bee pollen 4 DEG C
As seen from Table 3, when tea bee pollen stores 12 months at 4 DEG C, a, b value is all without notable change, although IC50 value rises
Height, but still there is relatively strong anti-oxidation vigor, illustrate tea bee pollen can at 4 DEG C fresh-keeping 1 year still comparatively fresh.
Claims (2)
1. the method for quick of a tea bee pollen freshness, it is characterised in that realized by following steps:
(1) by after freeze-dried for tea bee pollen to be measured, carry out mechanical breaking-wall method, cross the sieve of 100 mesh, weigh more than 2 grams of samples
It is laid in planchet mensuration；
(2) measure a, b value of tea bee pollen powder sample, independent mensuration 3 times with colour difference meter, average；
(3) freshness detection: if a value is below 13, b value is below 50, it is determined that this tea bee pollen is to be equivalent to more than 35 DEG C
High temperature storage more than 3 months, regards as stale.
The method the most according to claim 1 application in the quickly detection of tea bee pollen freshness.
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101021484A (en) * | 2007-02-08 | 2007-08-22 | 浙江大学 | Quick detection reagent for royal jelly freshness |
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2016
- 2016-06-20 CN CN201610452690.3A patent/CN106093320A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101021484A (en) * | 2007-02-08 | 2007-08-22 | 浙江大学 | Quick detection reagent for royal jelly freshness |
Non-Patent Citations (5)
| Title |
|---|
| MASAKI KAMAKURA 等: "Storage-dependent degradation of 57-kDa protein in royal jelly：a possible market for freshness", 《BIOSCI.BIOTECHNOL.BIOCHEM.》 * |
| 刘禹廷 等: "6种云杉属植物花粉活性测定", 《安徽农业科学》 * |
| 方小明 等: "荷花粉真空脉动干燥特性和干燥品质", 《农业工程学报》 * |
| 李福高 等: "花粉活性成分的提取与分离现状及展望", 《蜜蜂杂志(月刊)》 * |
| 齐旦旦: "蜂王浆蛋白质、多肽生物活性及储藏过程中品质变化研究", 《中国优秀硕士学位论文全文数据库农业科技辑》 * |
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Application publication date: 20161109 |
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