CN106086091A - A kind of itaconic acid concentrates the condensed water method for itaconic acid fermentation - Google Patents
A kind of itaconic acid concentrates the condensed water method for itaconic acid fermentation Download PDFInfo
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- CN106086091A CN106086091A CN201610430942.2A CN201610430942A CN106086091A CN 106086091 A CN106086091 A CN 106086091A CN 201610430942 A CN201610430942 A CN 201610430942A CN 106086091 A CN106086091 A CN 106086091A
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- fermentation
- itaconic acid
- water
- fermentation tank
- feso
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/44—Polycarboxylic acids
Abstract
The invention discloses a kind of itaconic acid and concentrate the condensed water method for itaconic acid fermentation, first take water that a certain amount of itaconic acid distills during condensing crystallizing, cool, after equipped with the exchange column of cation exchange resin, measure its pH, take the pH water more than 4.5 standby；Then by pH value more than 4.5 water be passed through in fermentation tank, and in fermentation tank add dispensing C6H12O6、NH4NO3, Semen Maydis pulp and FeSO4, obtain fermentation liquid；Fermentation tank containing fermentation liquid finally being put into shaking table, controls temperature 37 DEG C, rotating speed 210r/min, humidity 70 80%, ferment 72h, measures itaconic acid and residual sugar content through HPLC method.By comparing tap water from friendship with the concentration distilled water after processing in testing at 5L fermentation tank, the two includes that at itaconic acid fermentation the aspects such as fermentation period, fermenting acidity, fermentation residual sugar are the most suitable.
Description
Technical field
The present invention relates to itaconic acid fermentation preparing technical field, be specifically related to a kind of itaconic acid and concentrate condensed water for clothing health
The method of acid fermentation.
Background technology
Itaconic acid fermentation uses 300m mostly at present3Fermentation tank, needs substantial amounts of in the blending process of itaconic acid fermentation
Tap water, the use of tap water not only constitutes the cost of itaconic acid fermentation, and the use structure of the tap water to growing tension
Become serious crisis.Itaconic acid, during extracting, needs first to be concentrated by itaconic acid fermentation liquid, and can be by concentration process
Most of moisture in itaconic acid fermentation liquid are evaporated, and are as the most qualified discharge of sewage by this part water before,
Add the burden of sewage department.If can process this part water and be applied to produce, not only to itaconic acid fermentation cost
Reduce significant, and saving, the environmental conservation to tap water is the most significant.
Summary of the invention
The problem cannot being utilized effectively for the condensed water produced in itaconic acid concentration process in above-mentioned prior art,
The present invention proposes a kind of itaconic acid and concentrates the condensed water method for itaconic acid fermentation, and the method is not only effectively utilized clothing health
Acid condensation water, reduces production cost, and course of fermentation and fermenting acidity is unaffected.
Its technical solution includes:
A kind of method of itaconic acid fermentation, comprises the following steps successively:
A takes water that a certain amount of itaconic acid distills during condensing crystallizing, cools, through equipped with cation exchange tree
Measure its pH after the exchange column of fat, take the pH water more than 4.5, standby；
The water that step a resulting ph is more than 4.5 is passed through in fermentation tank by b, and adds dispensing C in fermentation tank6H12O6、
NH4NO3, Semen Maydis pulp and FeSO4, obtaining fermentation liquid, dispensing mass percent in fermentation liquid is: C6H12O613.5-14.5%,
NH4NO30.2-0.3%, Semen Maydis pulp 0.15% and FeSO40.01%, the pH adjusting fermentation liquid is 3.0；
Shaking table put into by the fermentation tank that step b is contained fermentation liquid by c, controls temperature 37 DEG C, rotating speed 210r/min, humidity 70-
80%, ferment 72h, measures itaconic acid and residual sugar content through HPLC method.
As a preferred version of the present invention, the water distilled instills described cation exchange resin from top to bottom
In.
As another preferred version of the present invention, take the pH water equal to 5.8 standby.
Preferably, dispensing mass percent in fermentation liquid is: C6H12O614%, NH4NO30.3%, Semen Maydis pulp
0.15% and FeSO40.01%.
The Advantageous Effects that the present invention is brought:
The present invention in itaconic acid fermentation, select itaconic acid concentrate after condensed water be raw material, pass it through equipped with sun from
Measuring its pH after the exchange column of sub-exchange resin, take the pH water more than 4.5, experimental verification distills when pH is more than 4.5
Water can substitute tap water completely for itaconic acid fermentation.
By tap water being compared with the concentration distilled water after ion exchange resin treatment in testing at 5L fermentation tank
Relatively, the two includes that at itaconic acid fermentation the aspects such as fermentation period, fermenting acidity, fermentation residual sugar are the most suitable.
The present invention had both been effectively utilized in the concentration process of itaconic acid the condensed water produced, and the fermentation the most not affecting itaconic acid is entered
Journey and fermenting acidity.
Detailed description of the invention
The present invention proposes a kind of itaconic acid and concentrates condensed water for the method for itaconic acid fermentation, and in order to make, the present invention's is excellent
Point, technical scheme are clearer, clear and definite, elaborate the present invention below in conjunction with specific embodiment.
Cation exchange resin selected by the present invention is weakly acidic resin, dispensing C6H12O6、NH4NO3, Semen Maydis pulp and
FeSO4All can be bought by commercial channel and obtain.
Embodiment 1:
The method of itaconic acid fermentation, specifically includes following steps:
Step one, big for D111 casement acidulous acrylic acid's cation exchanger resin is carried out loads ion after pretreatment hand over
Changing in post, remove air, draining to liquid level exceeds resin bed 2cm, and the sodium hydroxide of the 1mol/L adding resin volume 50 times is molten
Liquid, passes through resin bed with the flow velocity of 20ml/min；After with same flow velocity, be passed through pure water resin, until
Liquid in low side effluent is not till phenolphthalein detection becomes redness；The pending itaconic acid cooled is concentrated condensed water from upper
And in lower instillation ion exchange column, collect the liquid being disposed the pH value detecting this liquid, finally giving pH is pH4.5,
Three groups of solution of pH5.3 and pH5.6；
Step 2, three groups of water samples of pH4.5, pH5.3 and pH5.6 after ion hands over post post to process, in 5L fermentation tank,
Dispensing, dispensing C is carried out respectively with these three groups of water samples6H12O613.5%, NH4NO3Content is 0.25%, and Semen Maydis pulp content is
0.15%, FeSO4Content is 0.01%, adjusts pH to 3, controls temperature 37 DEG C, and rotating speed is 210r/min, after fermentation time 72h
Measuring through HPLC method, itaconic acid content is respectively 90.8g/L, 92.0g/L, 91.5g/L, and residual sugar content is respectively 0.44%,
0.36%, 0.38%.
Comparative example 1:
On 5L fermentation tank, tap water dispensing C6H12O613.5%, NH4NO3Content is 0.25%, and Semen Maydis pulp content is
0.15%, FeSO4Content is 0.01%, adjusts pH to 3, controls temperature 37 DEG C, and rotating speed is 210r/min, after fermentation time 72h
Measuring through HPLC method, itaconic acid content is 91.2g/L, residual sugar content is 0.39%.
Embodiment 2:
The method of itaconic acid fermentation, specifically includes following steps:
Load after big for D111 casement acidulous acrylic acid's cation exchanger resin is carried out pretreatment in ion exchange column,
Removing air, draining to liquid level exceeds resin bed 3cm, adds the sodium hydroxide solution of the 2mol/L of resin volume 50 times, with
The flow velocity of 25ml/min passes through resin bed, after with same flow velocity, be passed through pure water resin, until at low side stream
Go out the liquid in liquid till phenolphthalein detection does not becomes redness；The pending itaconic acid cooled is concentrated condensed water drip from top to bottom
Entering in this ion exchange column, collect the liquid being disposed the pH value detecting this liquid, finally giving pH is pH5.0, pH5.1
And three groups of solution of pH5.8；
Step 2, three groups of water samples of pH5.0, pH5.1 and pH5.8 after treatment on ion exchange columns, on 5L fermentation tank,
Dispensing C is carried out respectively with these three groups of water samples6H12O614%, NH4NO3Content is 0.3%, and Semen Maydis pulp content is 0.15%, FESO4
Content is 0.01%, adjusts pH to 3, controls temperature 37 DEG C, and rotating speed is 210r/min, measures through HPLC method after fermentation time 72h,
Itaconic acid content is respectively 94.0g/L, 94.7g/L, 95.1g/L, and residual sugar content is respectively 0.50%, 0.45%, 0.4%.
Comparative example 2:
On 5L fermentation tank, tap water dispensing C6H12O614%, NH4NO3Content is 0.3%, and Semen Maydis pulp content is
0.15%, FESO4Content is 0.01%, adjusts pH to 3, controls temperature 37 DEG C, and rotating speed is 210r/min, after fermentation time 72h
Measuring through HPLC method, itaconic acid content is 94.3g/L, residual sugar content is 0.48%.
It should be noted that any equivalent way that those skilled in the art are made under the teaching of this specification, or
Substantially variant all should be within the scope of the present invention.
Claims (4)
1. the method for an itaconic acid fermentation, it is characterised in that comprise the following steps successively:
A takes water that a certain amount of itaconic acid distills during condensing crystallizing, cools, through equipped with cation exchange resin
Measure its pH after exchange column, take the pH water more than 4.5 standby；
The water that step a resulting ph is more than 4.5 is passed through in fermentation tank by b, and adds dispensing C in fermentation tank6H12O6、NH4NO3、
Semen Maydis pulp and FeSO4, obtaining fermentation liquid, dispensing mass percent in fermentation liquid is: C6H12O613.5-14.5%, NH4NO3
0.2-0.3%, Semen Maydis pulp 0.15% and FeSO40.01%, the pH adjusting fermentation liquid is 3.0；
Shaking table put into by the fermentation tank that step b is contained fermentation liquid by c, controls temperature 37 DEG C, rotating speed 210r/min, humidity 70-80%,
Fermentation 72h, measures itaconic acid and residual sugar content through HPLC method.
The method of itaconic acid fermentation the most according to claim 1, it is characterised in that: in step a, the water distilled is from upper
And in the lower described cation exchange resin of instillation.
The method of itaconic acid fermentation the most according to claim 1, it is characterised in that: in step a, take the pH water equal to 5.8
Standby.
The method of itaconic acid fermentation the most according to claim 1, it is characterised in that: in step b, dispensing is in fermentation liquid
Mass percent is: C6H12O614%, NH4NO30.3%, Semen Maydis pulp 0.15% and FeSO40.01%.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106770734A (en) * | 2016-12-01 | 2017-05-31 | 科之杰新材料集团有限公司 | A kind of detection method of the itaconic acid residual quantity in polycarboxylate water-reducer |
| CN113321339A (en) * | 2021-05-06 | 2021-08-31 | 江南大学 | Method for producing fuel ethanol by recycling evaporated condensed water and reusing evaporated condensed water |
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| CN102452702A (en) * | 2010-10-15 | 2012-05-16 | 中粮生物化学（安徽）股份有限公司 | Recycling method of condensed water |
| CN103045671A (en) * | 2012-12-23 | 2013-04-17 | 菱花集团有限公司 | Recycling method of condensed water in monosodium glutamate fermentation |
| CN103468752A (en) * | 2013-04-24 | 2013-12-25 | 北京贯虹科技集团有限公司 | Preparation method of monomer itaconic acid |
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| CN101029319A (en) * | 2007-03-30 | 2007-09-05 | 菱花集团有限公司 | Environmental-protecting process for fermenting glutamic acid |
| CN102452702A (en) * | 2010-10-15 | 2012-05-16 | 中粮生物化学（安徽）股份有限公司 | Recycling method of condensed water |
| CN103045671A (en) * | 2012-12-23 | 2013-04-17 | 菱花集团有限公司 | Recycling method of condensed water in monosodium glutamate fermentation |
| CN103468752A (en) * | 2013-04-24 | 2013-12-25 | 北京贯虹科技集团有限公司 | Preparation method of monomer itaconic acid |
| CN203540090U (en) * | 2013-10-15 | 2014-04-16 | 济南华明生化有限公司 | Itaconic acid energy-saving-type four-effect concentrating and crystallizing device |
| CN105087390A (en) * | 2015-08-05 | 2015-11-25 | 中国林业科学研究院林产化学工业研究所 | High-yield itaconic acid strains and high-throughput screening method thereof based on deep-hole plate |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106770734A (en) * | 2016-12-01 | 2017-05-31 | 科之杰新材料集团有限公司 | A kind of detection method of the itaconic acid residual quantity in polycarboxylate water-reducer |
| CN113321339A (en) * | 2021-05-06 | 2021-08-31 | 江南大学 | Method for producing fuel ethanol by recycling evaporated condensed water and reusing evaporated condensed water |
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Application publication date: 20161109 |