CN106085848A - A kind of cell co-culture device and preparation method thereof, using method and application - Google Patents
A kind of cell co-culture device and preparation method thereof, using method and application Download PDFInfo
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Abstract
The invention provides a kind of cell co-culture device and preparation method thereof, using method and application, this cell co-culture device includes base plate, some sheet glass cultivated for cell it are placed with on base plate, shelter it is placed with on sheet glass, a part for each sheet glass is blocked by obstructions, being designated as region 2, the part that sheet glass is not blocked by obstructions is designated as region 1, and with for distinguishable region 1 and the labelling of region 2 scope on sheet glass.The cell co-culture device that the present invention provides, in use by specific cell is seeded in specific position, cell type can be determined according to inoculation position, need not again cell be carried out molecular marker, just can carry out the interactional research between different cell, the allogenic cell that can be used for studying between different conditions is influencing each other of bringing by directly contact, it may also be used for that studies that not allogenic cell brought by direct contact influences each other.
Description
Technical field
The invention belongs to biological cell culture experiment device field, relate to a kind of for studying impact between cell and cell
Cell co-culture device and preparation method thereof, using method and application.
Background technology
The organic whole that organism is made up of various kinds of cell, by influencing each other between different cells, Coordinated Play is made
With, maintain organ, tissue normal, focus that influencing each other between cell is always studied.Phase is there is between different cells
Mutually impact, such as: the intercellular communication between oligodendrocyte and neuronal cell affects the formation of myelin, myocardial cell with become
Influencing each other between fibrocyte participates in the growth course of heart, the interaction between dendritic cell and T lymphocyte
Participate in the antigen deduction process in immunoreation, etc..There is also between allogenic cell different conditions and influence each other, such as: regard
Apoptotic signal in nethike embrane layer can make the normal cell generation apoptosis around apoptotic cell at tissue internal diffusion；Myocardial infarction
During, dead signal can connect transmission by gap, makes the normal cell generation apoptosis or bad around dead myocardial cells
Extremely, etc..Influencing each other extensively between cell, playing a significant role under organism physiology and pathological state, be worth be
The research of system.
Currently for the impact between cell, experimental technique is a lot, mainly uses co-culture of cells, between research cell
Influence each other.The experimental technique of co-culture of cells can be divided into following a few class；(1) Transwell co-culture system.Experiment dress
Put and be divided into room and lower room, separated, upper indoor by one layer of polycarbonate membrane semipermeable membrane (semipermeable membrane aperture :～3 μm) therebetween
Cultivating a kind of cell, lower indoor cultivation another kind cell, cell self can not pass through semipermeable membrane, but emiocytosis various because of
Son can be the most penetrating；This experimental provision is used for the cytokine the studying a kind of emiocytosis effect to another kind of cell, no
Can be used for studying a kind of cell by cells contacting mode, the effect to another kind of cell.(2) two kinds of cells are at a culture plate
Interior growth.Two different kinds of cell is successively inoculated in a culture plate according to a certain percentage, after cultivating a period of time, to two
The marker protein of class cell is dyeed by SABC, distinguishes the variety classes cell in culture plate, observes and co-cultured
The impact of journey cell growth, this experimental technique is used for studying a kind of cell and by excreted factor or directly contacts, to another
Plant the impact of cell.(3) two kinds of cells successively grow in a culture plate.A kind of cell is inoculated in culture plate, cultivates one
After the section time, reach the fusion close to 80%, the more another kind of cell of the most a small amount of inoculation between cell, in culture plate, form two
Confluent monolayer cells, lower floor is the first cell, and upper strata is the second cell, and this experimental technique is used for studying between different cell by straight
Contact is on the impact between cell.(4) two kinds of cells grow in a culture plate.Two different kinds of cell is according to one
Certainty ratio is inoculated in a culture plate, after cultivating a period of time, two kinds of cell differentiation is collected by flow cytometry, with laggard
Row biochemical analysis, influencing each other between research cell.(5) use engineered method, co-culture after labeled cell.
After fluorescent protein labeling one of which cell, itself and other cell are cultivated in a culture plate, distinguish two kinds by fluorescence
Cell, observes a series of index subsequently, studies the impact between two kinds of cells.
Although there is multiple co-culture method, also a class co-cultures research is that current experimental technique can not meet
's.The problem that carry out study such as is presently required is: research green fluorescent protein (GFP) is between the myocardial cell contacted with each other
Diffusion process, and current co-culture method be not all suitable for this research.Reason is: (1) Transwell co-cultures body
System；The method problematically, the method is applicable to study the interaction between two kinds of cells, do not exist between two kinds of cells
Directly contact, is not therefore suitable for above-mentioned problem.(2) two kinds of cells are inoculated in a culture plate the most simultaneously；This
If method problematically, use this co-culture method, need in advance by the way of virus infects, by GFP import cardiac muscle
Intracellular, subsequently by infecting the myocardial cell of virus and the myocardial cell of uninfecting virus, co-culture in a culture plate, see
Examine the diffusion process of GFP albumen.The defect of the method is that late stage of culture cell occurs that green fluorescence has two kinds of situations, the first
Situation: infecting the cell that virus quantity is few, early stage GFP expression is few, can not be identified in early stage, viral through self-replication, after
Phase GFP expression increases, and cell there will be fluorescence；The second situation: early stage infects virus, expresses GFP and fluorescence occurs
Myocardial cell, after the cells contacting being uninfected by with surrounding, in several ways, GFP can from infect the cellular invasion of virus to
The cell of uninfecting virus, the cell of uninfecting virus also there will be fluorescence.This co-culture method cannot distinguish between there is fluorescence
Cell is the first situation, or the second situation, and therefore the method is not suitable for the research of above-mentioned problem.(3) two kinds of cells
Co-culture in bilevel mode；The method problematically, myocardial cell was cultivated after a period of time, not only can phase
Merging mutually, also can grow in the way of stacking, the time is the longest, and bilevel cell differentiation is the most inconspicuous, and therefore the method is not
The research of above-mentioned problem can be used for.(4) two kinds of cells are separated and collected by flow cytometry after co-culture of cells；Asking of the method
Topic is, the method can only distinguish the myocardial cell expressing GFP and the myocardial cell not expressing GFP, it is impossible to confirm that GFP derives from
Virus infects, or GFP is by other cellular invasion, and therefore the method cannot be used for the research of above-mentioned problem.(5) base is used
Because co-culturing after the method labeled cell of engineering；The method problematically, cell is carried out GFP and the second divides simultaneously
Sub-labelling, it is impossible to ensure that the second molecule is confined to the cell of virus infection all the time, it is possible to also can be by the side of cells contacting
Formula shifts, and therefore the method cannot be used for the research of above-mentioned problem.It is thus desirable to exploitation design is a set of can be used in above-mentioned problem
New cell co-culture device so that need not cell is carried out molecular marker, that can study between different conditions is of the same race
Cell is influencing each other of bringing by directly contact.
Summary of the invention
It is an object of the invention to provide a kind of cell co-culture device and preparation method thereof, using method and application, make
Need not during with this cell co-culture device cell is carried out molecular marker, the allogenic cell between different conditions can be studied and lead to
Cross and directly contact and influencing each other of bringing, the mutual shadow that allogenic cell is not brought by directly contact can also be studied simultaneously
Ring.
For reaching above-mentioned purpose, the technical solution used in the present invention is:
A kind of cell co-culture device, including base plate, base plate is placed with some sheet glass cultivated for cell, glass
Being placed with shelter on sheet, a part for each sheet glass is blocked by obstructions, and is designated as region 2, and sheet glass is not blocked thing
The part sheltered from is designated as region 1, and with for distinguishable region 1 and the labelling of region 2 scope on sheet glass.
Described base plate is the glass plate of surfacing, and sheet glass is coverslip, and shelter is microscope slide, coverslip or saturating
Gelatin band.
The manufacture method of described cell co-culture device, it specifically comprises the following steps that
Choose surfacing, clean base plate, base plate is placed some surfacings, clean sheet glass, and at glass
Put on for distinguishable region 1 and the labelling of region 2 scope on glass sheet, then shelter from region 2, then the place that carries out disinfection with shelter
Reason, i.e. obtains cell co-culture device.
The described mode being to use cobalt source to irradiate of disinfecting is sterilized.
The application in terms of cultivating two kinds of different cells of the described cell co-culture device.
Application in terms of described cell co-culture device allogenic cell under cultivating two kinds of different cell states.
The using method of described cell co-culture device, it specifically comprises the following steps that
1) aseptic cell co-culture device is put into culture dish, train in then cell A being seeded to culture dish
Support, then cell co-culture device is removed in culture dish, now uncovered on base plate in cell co-culture device region,
Cell A has all been sticked on region 1 on sheet glass and shelter；
2) shelter is removed from cell co-culture device, exposes the region 2 not sticked by cell A, then remove base plate,
Only retain sheet glass, now sticked cell A on the region 1 of sheet glass, region 2 does not sticks any cell；
3) being moved into by sheet glass in 24 orifice plates, in 24 orifice plates, the virus of gene X, incubated cell are carried in inoculation, make virus
Infection cell A, now the part cell A on sheet glass region 1 is infected, then changes into fresh by the culture fluid in 24 orifice plates
Culture fluid, removes virus free in culture fluid；
4) in 24 orifice plates, inoculating cell A cultivates again, has now all sticked on the region 1 of sheet glass and region 2
The cell A of second time inoculation, with the cell A not being infected and the first time that also stick inoculation for the first time on time domain 1
The cell A being infected of inoculation；
5) proceed cell in sheet glass moves into 24 new orifice plates to cultivate, the cell that last collecting zone 2 sticks
Carry out biochemical analysis, the impact on region 2 inner cell of the cell in analyzed area 1.
The using method of described cell co-culture device, it specifically comprises the following steps that
1) aseptic cell co-culture device is put into culture dish, train in then cell A being seeded to culture dish
Support, then cell co-culture device is removed in culture dish, now uncovered on base plate in cell co-culture device region,
Cell A has all been sticked on region 1 on sheet glass and shelter；
2) shelter is removed from cell co-culture device, exposes the region 2 not sticked by cell A, then remove base plate,
Only retain sheet glass, now sticked cell A on the region 1 of sheet glass, region 2 does not sticks any cell；
3) being moved into by sheet glass in 24 orifice plates, in 24 orifice plates, inoculating cell B cultivates, now the region 1 of sheet glass
Upper stick cell A and cell B simultaneously, region 2 has only sticked cell B；
4) proceed cell in sheet glass moves into 24 new orifice plates to cultivate, the cell that last collecting zone 2 sticks
Carry out biochemical analysis, the impact on region 2 inner cell of the cell in analyzed area 1.
Relative to prior art, the invention have the benefit that
The cell co-culture device simple in construction that the present invention provides, manufacture method is easy, and easily uses, and has
Advantage not available for 4 existing cell co-culture devices below, specific as follows:
1) cell co-culture device that the present invention provides may be used for studying the impact between two kinds of different cells: by cell
A is seeded in region 1, and cell B is seeded in region 2, can study the cell A impact on cell B, especially can study cell A
The impact by the way of direct exposing cell B, cell B produced；
2) cell co-culture device that the present invention provides can also study the impact between allogenic cell different conditions: by A
The cell C of state is seeded in region 1, and the cell C of B state is seeded in region 2, can study the cell C of A condition to B state
The impact of cell C, especially can be to B state in the way of the cell C of research A condition is by the cell C of directly contact B state
The impact that cell C produces；
3) cell co-culture device that the present invention provides need not be marked cell to be studied before use: because
The using method of the cell co-culture device according to the present invention, is seeded in specific position, finally in region 2 by specific cell
Interior cell is all same class, after determining position on the glass sheet, region 2 according to labelling, can determine sheet glass according to position
The type of upper cell, it is not necessary to marker protein or form by cell self determine cell type；
4) after making to be finished the cell co-culture device cultivation cell that the present invention provides, the cell in region 2 is received
Collection, can carry out the biochemical analysis being correlated with, and the protein expression of region 2 inner cell is affected by the cell in survey region 1: can be by
Cell in region 2 directly carries out fixation in situ, immunohistochemical staining analysis, the metamorphosis of survey region 2 cell, and egg
White in intracellular changes in distribution.
The allogenic cell that the cell co-culture device that the present invention provides can be used in studying between different conditions is by directly
Contact and influencing each other of bringing, that can also study that not allogenic cell brings by directly contacting influences each other simultaneously, it is possible to
Apply in terms of cultivating two kinds of different cells, it is also possible in terms of the allogenic cell under cultivating two kinds of different cell states
Apply, there is good practicality and be widely applied prospect.
Accompanying drawing explanation
The Making programme schematic diagram of the cell co-culture device that Fig. 1 provides for the present invention；
Fig. 2 is the pictorial diagram of the cell co-culture device that the present invention makes；
The use schematic flow sheet of the cell co-culture device that Fig. 3 provides for the present invention；
Fig. 4 is the diffusion process figure of GFP between myocyte；
Fig. 5 is GFP diffusion process figure between the myocardial cell contacted mutually.
Detailed description of the invention
The invention provides a kind of cell co-culture device, be applied to study influencing each other between different cell.Different
Can be influenced each other between the cell of kind by the way of contact the function of cell, and in this type of is studied, part research uses
The mode of fluorescence molecule labelling distinguishes two kinds of cells, observes the impact on another kind of cell of a kind of cell；But another part grinds
In studying carefully, be not suitable for using the mode of fluorescence molecule labelling to distinguish two kinds of cells, this type of carrying out of studying is caused certain difficulty.
The cell co-culture device that the present invention provides, is seeded in specific position by specific cell, determines cell according to inoculation position
Type, it is not necessary to again cell is carried out molecular marker, so that it may carry out the interactional correlational study between different cell.
Below in conjunction with the accompanying drawings the present invention is described in further details, described in be explanation of the invention rather than restriction.
As it is shown in figure 1, the cell co-culture device that the present invention provides, including base plate, base plate is placed with some for carefully
The sheet glass that born of the same parents cultivate, sheet glass is placed with shelter, and a part for each sheet glass is blocked by obstructions, and is designated as region
2, the part that sheet glass is not blocked by obstructions is designated as region 1, and with for distinguishable region 1 and region 2 model on sheet glass
The labelling enclosed.Wherein base plate requires nothing more than surfacing, can select rectangle coverslip 1. or plastic plate, metallic plate etc.；
2. sheet glass can select circular lid slide；As long as shelter can reach to shelter from the function of segment glass sheet, Ke Yixuan
With rectangle coverslip or adhesive tape 3..
As depicted in figs. 1 and 2, the present invention specifically includes following steps when making cell co-culture device:
1) the rectangle coverslip of clean surface is chosen 1. (as shown in Figure 1A)；
2) 1. go up at rectangle coverslip and uniformly place 6 circular lid slides 2., 6 circular lid slides 2. on stamp mark
Note (as shown in Figure 1B)；
3) labelling 2. gone up according to circular lid slide, is 2. divided into two regions by circular lid slide: region 1 and region 2 (as
Shown in Fig. 1 C)；
4) with rectangle coverslip or adhesive tape 3. overlay area 2 (as shown in figure ip)；
5) device is sterilized by cobalt source radiation modality, i.e. complete the making of cell co-culture device, the cell made
The pictorial diagram of co-culture device is as shown in Figure 2.
As it is shown on figure 3, with research green fluorescent protein (GFP) diffusion process between the myocardial cell contacted with each other be
Example illustrates the using method of the cell co-culture device that the present invention provides, and it specifically comprises the following steps that
1) aseptic cell co-culture device is put into culture dish (as shown in Figure 3A)；
2) neonatal rat myocardial cell is seeded in culture dish, cultivates 24h, as shown in Figure 3 B, now in culture dish, rectangle
3. 1. coverslip goes up uncovered region, 2. circular lid slide is gone up region 1 and adhesive tape are gone up and have all been sticked Neonatal myocardial
Cell；
3) cell co-culture device is removed in culture dish, as shown in Figure 3 C, now rectangle coverslip 1. go up not by
3. region 1 and adhesive tape that 2. the region of covering, circular lid slide are gone up are gone up and have still been sticked neonatal rat myocardial cell；
4) 3. adhesive tape is removed from cell co-culture device, as shown in Figure 3 D, now on cell co-culture device
3. the region covered by adhesive tape does not stick neonatal rat myocardial cell, and other regions have still sticked neonatal rat myocardial cell；Figure
3E is the pictorial diagram that Fig. 3 D is corresponding, is able to verify that, by experiment, the region 1 and region 2 that 2. 3. circular lid slide gone up by adhesive tape
Cell stick without impact；
5) 1. rectangle coverslip is removed from cell co-culture device, only retain 6 circular lid slides 2., such as Fig. 3 F
Shown in, the region 1 that now 2. circular lid slide is gone up sticks neonatal rat myocardial cell, region 2 has not sticked Neonatal myocardial thin
Born of the same parents；
6) 2. 6 circular lid slides are moved in 6 holes of 24 orifice plates, as shown in Figure 3 G；
7) in 24 orifice plates, the virus of GFP gene is carried in inoculation, hatches 24h, makes virus infect neonatal rat myocardial cell, such as figure
Shown in 3H, neonatal rat myocardial cell exists only on the region 1 that 2. circular lid slide is gone up, and part neonatal rat myocardial cell is felt by virus
Dye, remainder neonatal rat myocardial cell is not infected；
8) culture fluid in 24 orifice plates is changed fresh medium into, remove virus free in liquid；
9) inoculation neonatal rat myocardial cell (second time inoculation myocardial cell) in 24 orifice plates, cultivation 24h, as shown in fig. 31,
Now all stick the neonatal rat myocardial cell of second time inoculation in 24 orifice plates and the region 1 2. gone up of circular lid slide and region 2；
10) 6 circular lid slides are moved into 24 new orifice plates, as shown in figure 3j, on the region 2 that 2. circular lid slide is gone up only
Stick the neonatal rat myocardial cell of second time inoculation, and on region 1, docile has the neonatal rat not being infected of inoculation for the first time
Myocardial cell, the neonatal rat myocardial cell being infected of inoculation for the first time and the neonatal rat myocardial cell of second time inoculation；And
Existing at region 1 and the intersection in region 2, the Neonatal myocardial being infected of the inoculation for the first time of some in region 1 is thin
The situation that the neonatal rat myocardial cell that born of the same parents inoculate with some second time in region 2 directly contacts with each other；
11) cell continues to cultivate 12d；So far, the cell in circular lid slide region 1 2. includes 3 classes: inoculate for the first time
Myocardial cell (infect virus), the myocardial cell (uninfecting virus) of inoculation for the first time, the myocardial cell of second time inoculation；Circle
Cell in shape coverslip 2. region 2 is the myocardial cell of second time inoculation.
With fluorescence microscope Real Time Observation cell state in circular lid slide 2. region 2, and the GFP in region 2
Expression process.There is cells contacting in the cell in region 1 and region 2 at demarcation line, there is cell connection between this two parts cell
System's (as shown in figure 3j).Because the cell in region 2 is from not in contact with the most viral, cell self necessarily will not express GFP, region 1
Interior part cell can express green fluorescence；After cultivating 12d, if being found that the cardiac muscle expressing green fluorescence in region 2
Cell, then it is believed that GFP must be the cell in region 1 passes over, it was demonstrated that GFP can pass through certain between cell
Mode is transmitted, and concrete experimental result is as shown in Figure 4, Figure 5.
Fig. 4 gives the diffusion process of GFP between myocardial cell, and wherein A-F is cell fluorescence microphotograph, and A '-F ' is
Cell difference microphotograph.Below dotted line Boundary is region 1, and more than dotted line Boundary is region 2.Can be seen that cell
After cultivating 12d, green fluorescence has diffused to the Frontier in region 2.
Fig. 5 gives the diffusion process of GFP between the myocardial cell contacted with each other, and wherein cell 1 is positioned at region 1, cell 2
It is positioned at region 2, and cell 1 and cell 2 contact with each other.After cultivating 8d, cell 1 occurs that fluorescence do not occur in fluorescence, cell 2；And
After cultivating 12d, all there is fluorescence in cell 1 and cell 2.
The advantage of the cell co-culture device that the present invention provides is: 1) can study the impact between two kinds of cells；Carefully
Born of the same parents A is seeded in region 1, and cell B is seeded in region 2, studies the cell A impact on cell B.2) allogenic cell can be studied different
Impact between state；The cell of A condition is seeded in region 1, and the cell of B state is seeded in region 2, the cell of research A condition
Impact cell to B state.3) need not cell is marked；Cell in region 2 is all same class, determines that region 2 exists
Behind position on coverslip, i.e. can determine that the type of cell according to position, it is not necessary to by marker protein or the form of cell self
Determine cell type.4) cell in region 2 is after collecting, and can carry out the biochemical analysis being correlated with, thin in survey region 1
The protein expression of region 2 inner cell is affected by born of the same parents；Cell in region 2 directly carries out fixation in situ, and immunohistochemical staining divides
Analysis, the metamorphosis of survey region 2 cell, and albumen is in intracellular changes in distribution.These 4 be current it has been reported that
Advantage not available for cell co-culture device.But the cell co-culture device that the present invention provides can not get rid of paracrine to carefully
The impact of born of the same parents, only focuses on the impact by direct way of contact research cell by cell, therefore thin use the present invention to provide
Before born of the same parents' co-culture device, it should first carry out transwell co-culture experiments, get rid of paracrine impact between cell.
Claims (8)
1. a cell co-culture device, it is characterised in that: include base plate, base plate is placed with some glass cultivated for cell
Glass sheet, sheet glass is placed with shelter, and a part for each sheet glass is blocked by obstructions, and is designated as region 2, and sheet glass is not
The part being blocked by obstructions is designated as region 1, and with for distinguishable region 1 and the labelling of region 2 scope on sheet glass.
Cell co-culture device the most according to claim 1, it is characterised in that: described base plate is the glass of surfacing
Plate, sheet glass is coverslip, and shelter is microscope slide, coverslip or adhesive tape.
3. the manufacture method of the cell co-culture device described in claim 1 or 2, it is characterised in that it specifically comprises the following steps that
Choose surfacing, clean base plate, base plate is placed some surfacings, clean sheet glass, and at sheet glass
On put on for distinguishable region 1 and the labelling of region 2 scope, then shelter from region 2, then disinfection with shelter,
I.e. obtain cell co-culture device.
The manufacture method of cell co-culture device the most according to claim 3, it is characterised in that: described disinfecting is
The mode using cobalt source to irradiate is sterilized.
5. the application in terms of cultivating two kinds of different cells of the cell co-culture device described in claim 1 or 2.
6. in terms of the allogenic cell under cultivating two kinds of different cell states of the cell co-culture device described in claim 1 or 2
Application.
7. the using method of the cell co-culture device described in claim 1 or 2, it is characterised in that it specifically comprises the following steps that
1) aseptic cell co-culture device is put into culture dish, cultivate in then cell A being seeded to culture dish, then
Cell co-culture device is removed in culture dish, now uncovered on base plate in cell co-culture device region, glass
Cell A has all been sticked on region 1 on sheet and shelter；
2) shelter is removed from cell co-culture device, expose the region 2 not sticked by cell A, then remove base plate, only protect
Stay sheet glass, now sticked cell A on the region 1 of sheet glass, region 2 does not sticks any cell；
3) being moved into by sheet glass in 24 orifice plates, in 24 orifice plates, the virus of gene X, incubated cell are carried in inoculation, make virus infection
Cell A, now the part cell A on sheet glass region 1 is infected, then changes the culture fluid in 24 orifice plates into fresh cultured
Liquid, removes virus free in culture fluid；
4) in 24 orifice plates, inoculating cell A cultivates again, has now all sticked second on the region 1 of sheet glass and region 2
The cell A of secondary inoculation, with the cell A not being infected and the inoculation for the first time that also stick inoculation for the first time on time domain 1
The cell A being infected；
5) proceeding cell in sheet glass moves into 24 new orifice plates to cultivate, the cell that last collecting zone 2 sticks is carried out
Biochemical analysis, the impact on region 2 inner cell of the cell in analyzed area 1.
8. the using method of the cell co-culture device described in claim 1 or 2, it is characterised in that it specifically comprises the following steps that
1) aseptic cell co-culture device is put into culture dish, cultivate in then cell A being seeded to culture dish, then
Cell co-culture device is removed in culture dish, now uncovered on base plate in cell co-culture device region, glass
Cell A has all been sticked on region 1 on sheet and shelter；
2) shelter is removed from cell co-culture device, expose the region 2 not sticked by cell A, then remove base plate, only protect
Stay sheet glass, now sticked cell A on the region 1 of sheet glass, region 2 does not sticks any cell；
3) being moved into by sheet glass in 24 orifice plates, in 24 orifice plates, inoculating cell B cultivates, now same on the region 1 of sheet glass
Time sticked cell A and cell B, region 2 has only sticked cell B；
4) proceeding cell in sheet glass moves into 24 new orifice plates to cultivate, the cell that last collecting zone 2 sticks is carried out
Biochemical analysis, the impact on region 2 inner cell of the cell in analyzed area 1.
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| CN110724637A (en) * | 2019-11-22 | 2020-01-24 | 南通大学 | Glass slide system for direct contact cell co-culture and working method thereof |
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| CN201737950U (en) * | 2010-07-29 | 2011-02-09 | 上海中医药大学 | Special experimental apparatus for cell growing on cover glass |
| CN102273431A (en) * | 2011-06-01 | 2011-12-14 | 深圳大学 | Method for co-culturing freshwater rotifers and chlorella vulgaris |
| CN203999635U (en) * | 2014-07-17 | 2014-12-10 | 天津医科大学总医院 | Detachable cell climbing sheet culturing plate |
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| CN201737950U (en) * | 2010-07-29 | 2011-02-09 | 上海中医药大学 | Special experimental apparatus for cell growing on cover glass |
| CN102273431A (en) * | 2011-06-01 | 2011-12-14 | 深圳大学 | Method for co-culturing freshwater rotifers and chlorella vulgaris |
| CN203999635U (en) * | 2014-07-17 | 2014-12-10 | 天津医科大学总医院 | Detachable cell climbing sheet culturing plate |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110724637A (en) * | 2019-11-22 | 2020-01-24 | 南通大学 | Glass slide system for direct contact cell co-culture and working method thereof |
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| CN106085848B (en) | 2019-06-14 |
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