CN106075594B - A kind of Thermal inactive nano-fiber tubular scaffold and preparation method thereof - Google Patents
A kind of Thermal inactive nano-fiber tubular scaffold and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/507—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials for artificial blood vessels
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
- A61L27/3625—Vascular tissue, e.g. heart valves
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/12—Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/40—Preparation and treatment of biological tissue for implantation, e.g. decellularisation, cross-linking
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- Health & Medical Sciences (AREA)
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- Chemical & Material Sciences (AREA)
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Abstract
The invention discloses a kind of Thermal inactive nanofiber small-caliber tubular supports and preparation method thereof of acellular matrix enhancing.The preparation method is that: healthy animal aorta is taken, de- cell processing is carried out；The resulting matrix of de- cell processing is placed in surface plate, ultrapure water is added dropwise to whole matrix are flooded, is put into refrigerator freezing；Nanofiber will be spun into the stainless steel central spindle of mold, it is dry that nanofiber and central spindle are integrally spun into vacuum oven；Tubulose matrix is covered into the outer layer in nanofiber, as mechanics enhancement layer；The compound double-layer scaffold together with central spindle is assembled in mold, solution-cast, processing is carried out, obtains tubular bracket.Tubular bracket prepared by the present invention has good mechanical property, burst strength and biocompatibility, in transfer operation to human body without hypersensitivity, there is affinity with the intracorporal blood of people and perienchyma simultaneously, had a good application prospect in the inside and outside section's prosthesis of heart.
Description
Technical field
The present invention relates to a kind of small-caliber tubular support and preparation method thereof technical fields, more particularly to a kind of de- cell
Thermal inactive nanofiber small-caliber tubular support of matrix enhancement and preparation method thereof.
Background technique
It is cardiovascular that small-caliber vascular (SDVG, D < 6mm) is clinically used in coronary artery and peripheral blood vessel lesion etc.
It is in great demand in terms of disease treatment, the demand of global artificial blood vessel is 1,000,000/year, and current actual amount is 30
Ten thousand/year, only in the U.S., the quantity of average annual opening revascularization operation just reaches 250,000/year.But artificial blood vessel, especially
It is that small-caliber vascular not only lacks growth activity after being transplanted to human body, but also is also less prone to carry out adaptability with host's ambient enviroment
Adjustment matching, it is easy to lead to thrombus.Preparing internal layer by organizational project and regeneration medicine technology being capable of quick endothelialization, outer layer
With the intravascular stent that can promote the long microstructure such as of smooth muscle cell that there is good mechanics compliance simultaneously, ability again
Enough requirements for meeting mechanical property clinically excellent to small-caliber vascular stent biocompatibility, patency.Simple synthesis
The blood vessel of high molecular material preparation has disadvantages that, such as: thrombus easy to form, endometrial hyperplasia, calcification and causing chronic inflammation, together
When the potential without growth.
De- cell xenografts be revascularization reconstruction in a kind of most common organizational project substitute, effectively at
Dividing is collagen and elastin laminin, is natural material ingredient possessed by human body.The nanofiber of electrostatic spinning technique preparation
Although tubular bracket has certain mechanical property, but due to having too small hole, smooth muscle cell can not be grown into, Jin Jintong
It crosses physics and sticks and be easy to fall off, this is just needed through knitting skill and Thermal inactive technology combination electrostatic spinning technique, point
Functions of modulesization assigns intravascular stent, makes it have multilayered structure, Each performs its own functions, reaches clinical transplantation requirement.
Electrostatic spinning technique is widely used in preparing tissue engineering bracket, and knitting skill is applied and prepares tubulose branch
The report of frame also has document report.Since Thermal inactive technology is when preparing nanofiber, selectable limited material system,
Though being so widely applied not as good as the above two, preparation field of stents is blended with other materials also good application.But so far
Until, small-caliber tubular support is prepared using the method that electrostatic spinning technique, knitting skill and Thermal inactive technology combine
Report but not yet occurs.Therefore it is prepared by this three kinds of technologies and a kind of mature integrates mechanical property and biocompatibility
Multistage functionalization structure tubular bracket, will have huge economic benefit.
Summary of the invention
The problem to be solved by the invention is to provide a kind of Thermal inactive nanofiber osculums of acellular matrix enhancing
Diameter tubular bracket and preparation method thereof.
To solve the above-mentioned problems, the technical scheme adopted by the invention is that: a kind of Thermal inactive nano-fiber tubular
The preparation method of bracket, which comprises the following steps:
Step 1): taking healthy animal aorta, carries out de- cell processing, and is immersed in 0.85%~0.9% physiology salt
It is spare in water；
Step 2): the resulting matrix of cell processing will be taken off in step 1) and will be placed in surface plate, it is complete to flooding that ultrapure water is added dropwise
Portion's matrix, is put into -80 DEG C~-60 DEG C refrigerator freezings 48~72 hours, is freeze-dried 48~60 hours after taking-up；
Step 3): the nanofiber of 0.06~0.1mm thickness, nanofiber and central spindle will be spun into the stainless steel central spindle of mold
It is dry that entirety is spun into vacuum oven；
Tubulose matrix obtained in step 2): being covered the outer layer of the nanofiber made from step 3) by step 4), as power
Learn enhancement layer；
Step 5): the double-layer scaffold together with central spindle compound in step 4) is assembled in mold, carries out solution-cast, place
Reason, obtains tubular bracket.
Preferably, cell processing is taken off in the step 1) specifically: internal diameter is 1~6mm, the aorta of long 30~40mm from
With the PBS buffer solution hydro-peening 10~20 dual anti-containing 0.5%~1% penicillin/streptomycin after the abdomen taking-up of healthy animal
It is secondary, until surface is without pollutants such as bloodstain.
Preferably, the aorta is derived from 8 months big healthy animals.
Preferably, the de- cell process specifically: the abdominal aorta cleaned up is immersed in be mixed equipped with 50mL PBS
In the conical flask of solution, preservative film sealing is put into CO2De- cell solution is every other day changed once in isothermal vibration 7 days in shaking table.
It is highly preferred that the PBS mixed solution was prepared by following raw material according to parts by weight:
Preferably, nanofiber spinning solution is made of the raw material of following parts by weight meter in the step 3):
100 parts of hexafluoroisopropanol；
7~9.8 parts of lactic acid-caprol acton copolymer (PLCL)；
Poly- 0.2~3 part of decanedioic acid glyceride (PGS).
Preferably, it is made of by the solution of casting the raw material based on following parts by weight in the step 5):
Preferably, the specific steps are as follows:
PLLA, PGS and PLCL material mixing that certain mass is weighed on assay balance, are dissolved under the conditions of 50~60 DEG C
In tetrahydrofuran (THF), stirring and dissolving to acquisition mass volume ratio is the clarification uniform solution of 8~10 (g/mL)；It will then mix
Close the polytetrafluoroethyl-ne of the specific model with layers of nanofibers and acellular matrix layer assembled before solution is cast to rapidly
In alkene mold, separate it mutually under the conditions of being placed on -80 DEG C rapidly, standing time 24 hours or more；Mold is taken out later, is decorporated
It is immersed in after the shell of mold in 0 DEG C of ultrapure mixture of ice and water, tubular bracket is taken off into continuation from the axle center of mold after five minutes
It is immersed in 0 DEG C of ultrapure mixture of ice and water, a water coke slurry solvent was changed every 6 hours 48~96 hours, taken from deionized water
48 hours or more are freeze-dried after tubular bracket out to get three-layer pipe shape bracket is arrived.
It is prepared the present invention also provides a kind of preparation method using above-mentioned Thermal inactive nano-fiber tubular scaffold
Thermal inactive nano-fiber tubular scaffold.
By electrostatic spinning technique preparation have artificial cell outgrowth matrix nanofiber as with contacting blood
Internal layer, the supporting layer that middle layer is made of acellular matrix, outer layer are passed through thermotropic by tri- kinds of degradation materials of PLLA, PGS, PLCL
The large hole nano fiber composition of phase detachment technique preparation.
Compared with prior art, the beneficial effects of the invention are as follows the sticking of spinning material PGS Human Umbilical Vein Endothelial Cells used, be proliferated
There is good facilitation with migration, be more advantageous to endothelial cell and quickly cover with man-made support, to realize quick endothelialization, together
When and PGS be blended PLCL can PGS degradation after play the role of support endodermis；The acellular matrix in middle layer is natural
Human body intrinsic collagen and elastin laminin, the overall mechanics supporting layer as tubular bracket；Outer layer is Thermal inactive system
Standby large hole nano fibrous layer, guidance promote smooth muscle fast-growth, to reach bionical.It is this that there is tissue phase well
The small-caliber vascular stent that is prepared as developing of the compound engineering blood vessel of capacitive and biomechanical property provides a kind of letter
Single and effective technology of preparing thinking.
Detailed description of the invention
Fig. 1 is the cross-sectional view of Thermal inactive nano-fiber tubular scaffold prepared by the present invention；
Fig. 2 is the schematic diagram that completely detachable tubular bracket prepares mold；
Fig. 3 is the scanning electron microscope (SEM) photograph of the small-caliber tubular support of different nanofibers preparation；
Wherein, 1 is Thermal inactive nanofiber, and 2 be Electrospun nano-fibers.
Specific embodiment
In order to make the present invention more obvious and understandable, hereby with preferred embodiment, and attached drawing is cooperated to be described in detail below.
What embodiment 1 and embodiment 2 used prepares mold as shown in Fig. 2, including for received mold central spindle 1 and set
Mold outer sheath 4 on the outside of it, the both ends of mold central spindle 1 are respectively equipped with mold leakage preventing plug 1 and mold leakage preventing plug 23.
Embodiment 1
Taking 8 months big bores is the health pig aorta pectoralis of 3mm or so, carries out de- cell processing, and be immersed in 0.9%
Physiological saline in it is spare；Acellular matrix resulting after processing is placed in surface plate, ultrapure water is added dropwise to flooding whole bases
Matter is put into -80 DEG C of refrigerator freezings 60 hours, is freeze-dried after taking-up 60 hours, -20 DEG C of refrigerations are spare.Divide on assay balance
Also known as 0.4gPLLA, 0.2gPGS and 0.4gPLCL material is taken to mix, 10mL tetrahydrofuran solvent is added, sealed membrane is close
Screw socket reaction flask is sealed, is placed in 60 DEG C of water-baths, magnetic agitation is 10% clarification uniform solution to mass/volume score is obtained.With
The specific model with layers of nanofibers and acellular matrix layer assembled before mixed solution is cast to rapidly afterwards is gathered
In Teflon mold, separate it mutually under the conditions of being placed on -80 DEG C rapidly, standing time 24 hours.Mold is taken out later, is moved back
Be immersed in 0 DEG C of ultrapure mixture of ice and water after removing the shell of mold, tubular bracket is taken off from the axle center of mold after five minutes after
It is continuous to be immersed in 0 DEG C of ultrapure mixture of ice and water, a water coke slurry solvent was changed every 6 hours 48 hours, take out from deionized water
It is freeze-dried 24 hours after tubular bracket.By cell seeding technology, endothelial cell, bracket outer plantation are planted in internal stent
Smooth muscle cell obtains the three-layer pipe shape bracket that internal diameter is 3mm, after tube wall is 1mm through culture after a period of time.
Embodiment 2
Taking 8 months big bores is the healthy SD abdominal aorta of 2.2mm or so, carries out de- cell processing, and be immersed in
It is spare in 0.9% physiological saline；Acellular matrix resulting after processing is placed in surface plate, it is complete to flooding that ultrapure water is added dropwise
Portion's matrix is put into -80 DEG C of refrigerator freezings 48 hours, is freeze-dried after taking-up 48 hours, -20 DEG C of refrigerations are spare；It will be in mold
Diameter is the nanofiber that 0.1mm thickness is spun in 2mm stainless steel central spindle, and it is dry that nanofiber and central spindle are integrally put into vacuum oven
It is dry；The de- cell tubulose matrix taking-up of refrigeration is covered to be had in nanofiber central spindle in spinning, as mechanics enhancement layer；In assay balance
Upper 0.3gPLLA, 0.1gPGS and 0.6g PLCL material that weighs respectively mixes, and 10mL tetrahydrofuran solvent, sealing is added
Film seals screw socket reaction flask, is placed in 60 DEG C of water-baths, and magnetic agitation is that 10% clarification is uniform molten to mass/volume score is obtained
Liquid.The specific model with layers of nanofibers and acellular matrix layer assembled before then mixed solution is cast to rapidly
Teflon mould in, separate it mutually under the conditions of being placed on -80 DEG C rapidly, standing time 48 hours.Mould is taken out later
Have, is immersed in 0 DEG C of ultrapure mixture of ice and water after the shell for mold of decorporating, after five minutes by tubular bracket from the axle center of mold
It takes off and continues to be immersed in 0 DEG C of ultrapure mixture of ice and water, a water coke slurry solvent was changed every 6 hours 48 hours, from deionized water
It is freeze-dried 48 hours after middle taking-up tubular bracket.Through cell seeding technology, outside internal stent plantation endothelial cell, bracket
Smooth muscle cell is planted in portion, obtains the three-layer pipe shape bracket that internal diameter is 2mm, after tube wall is 1mm through culture after a period of time.
Thermal inactive nano-fiber tubular scaffold produced by the present invention is used as shown in Figure 1, internal layer is Static Spinning nanometer
Fiber, middle layer are acellular matrix, outer layer Thermal inactive nanofiber.
Claims (7)
1. a kind of preparation method of Thermal inactive nano-fiber tubular scaffold, which comprises the following steps:
Step 1): taking healthy animal aorta, carries out de- cell processing, and be immersed in 0.85%~0.9% physiological saline
It is spare；
Step 2): will take off cell and handle resulting matrix and be placed in surface plate in step 1), ultrapure water is added dropwise to flooding whole bases
Matter, is put into -80 DEG C~-60 DEG C refrigerator freezings 48~72 hours, is freeze-dried 48~60 hours after taking-up；
Step 3): will be spun into the nanofiber of 0.06~0.1mm thickness in the stainless steel central spindle of mold, nanofiber and central spindle are whole
It is dry to be spun into vacuum oven；
Tubulose matrix obtained in step 2): being covered the outer layer of the nanofiber made from step 3) by step 4), is increased as mechanics
Strong layer；
Step 5): the double-layer scaffold together with central spindle compound in step 4) is assembled in mold, carries out solution-cast, processing,
Obtain tubular bracket；
Nanofiber spinning solution is made of the raw material of following parts by weight meter in the step 3):
100 parts of hexafluoroisopropanol；
7~9.8 parts of lactic acid-caprol acton copolymer；
0.2~3 part of poly- decanedioic acid glyceride；
It is made of by the solution of casting the raw material based on following parts by weight in the step 5):
100 parts of tetrahydrofuran；
2~6 parts of Poly L-lactic acid；
0.2~3 part of poly- decanedioic acid glyceride；
2~6 parts of lactic acid-caprol acton copolymer.
2. the preparation method of Thermal inactive nano-fiber tubular scaffold as described in claim 1, which is characterized in that the step
It is rapid 1) in take off cell processing specifically: internal diameter is 1~6mm, the aorta of long 30~40mm is after the taking-up of the abdomen of healthy animal
With containing dual anti-PBS buffer solution hydro-peening 10~20 times of 0.5%~1% penicillin/streptomycin, until surface is polluted without bloodstain
Object.
3. the preparation method of Thermal inactive nano-fiber tubular scaffold as claimed in claim 1 or 2, which is characterized in that institute
It states aorta and is derived from 8 months big healthy animals.
4. the preparation method of Thermal inactive nano-fiber tubular scaffold as claimed in claim 1 or 2, which is characterized in that institute
State de- cell process specifically: the abdominal aorta cleaned up is immersed in the conical flask equipped with 50mL PBS mixed solution, is protected
Fresh film sealing, is put into CO2De- cell solution is every other day changed once in isothermal vibration 7 days in shaking table.
5. the preparation method of Thermal inactive nano-fiber tubular scaffold as claimed in claim 4, which is characterized in that described
PBS mixed solution is by following raw material preparation according to parts by weight:
6. the preparation method of Thermal inactive nano-fiber tubular scaffold as described in claim 1, which is characterized in that specific step
It is rapid as follows:
The mixing of PLLA, PGS and PLCL material is weighed on assay balance, is dissolved in tetrahydrofuran, is stirred under the conditions of 50~60 DEG C
Mix the clarification uniform solution for being dissolved to and obtaining that mass volume ratio is 8~10g/mL；Before then mixed solution is cast to rapidly
In the Teflon mould with layers of nanofibers and acellular matrix layer assembled, it is placed under the conditions of -80 DEG C rapidly
Separate it mutually, standing time 24 hours or more；Mold is taken out later, and 0 DEG C of ultrapure ice water is immersed in after the shell for mold of decorporating
In mixture, after five minutes tubular bracket is continued to be immersed in 0 DEG C of ultrapure mixture of ice and water, often from taking off on the axle center of mold
A water coke slurry solvent was changed every 6 hours 48~96 hours, from deionized water take out tubular bracket after be freeze-dried 48 hours with
On to get arrive three-layer pipe shape bracket.
7. a kind of preparation method system using Thermal inactive nano-fiber tubular scaffold as claimed in any one of claims 1 to 6
Standby Thermal inactive nano-fiber tubular scaffold.
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| CN106902389A (en) * | 2017-01-18 | 2017-06-30 | 烟台正海生物科技股份有限公司 | Modified xenogenesis acellular nerve graft thing of a kind of nanofiber surface and preparation method thereof |
| CN108478863A (en) * | 2018-04-24 | 2018-09-04 | 重庆大学 | The preparation method and products thereof of compound small-caliber artificial blood vessel |
| CN108904886B (en) * | 2018-10-15 | 2020-07-17 | 四川大学 | Double-layer stent containing PEG (polyethylene glycol) grafted chitosan and preparation method thereof |
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| KR20120098228A (en) * | 2011-02-28 | 2012-09-05 | 부산대학교 산학협력단 | Preparation method of stent for photodynamic stent |
| CN102784015A (en) * | 2012-08-30 | 2012-11-21 | 广州迈普再生医学科技有限公司 | Artificial blood vessel loaded with pseudo-ginseng medicines, and preparation method and application for artificial blood vessel |
| CN104689382A (en) * | 2015-03-18 | 2015-06-10 | 东华大学 | Braiding-reinforced nano-fiber small-caliber intravascular stent, and preparation and application thereof |
| CN105031735A (en) * | 2015-06-24 | 2015-11-11 | 东华大学 | Small-caliber artificial blood vessel with three-layer composite structure, and preparation method of small-caliber artificial blood vessel |
| CN105457101A (en) * | 2015-12-17 | 2016-04-06 | 华南理工大学 | Preparation method of small-caliber intravascular stent of three-layer structure |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20120098228A (en) * | 2011-02-28 | 2012-09-05 | 부산대학교 산학협력단 | Preparation method of stent for photodynamic stent |
| CN102784015A (en) * | 2012-08-30 | 2012-11-21 | 广州迈普再生医学科技有限公司 | Artificial blood vessel loaded with pseudo-ginseng medicines, and preparation method and application for artificial blood vessel |
| CN104689382A (en) * | 2015-03-18 | 2015-06-10 | 东华大学 | Braiding-reinforced nano-fiber small-caliber intravascular stent, and preparation and application thereof |
| CN105031735A (en) * | 2015-06-24 | 2015-11-11 | 东华大学 | Small-caliber artificial blood vessel with three-layer composite structure, and preparation method of small-caliber artificial blood vessel |
| CN105457101A (en) * | 2015-12-17 | 2016-04-06 | 华南理工大学 | Preparation method of small-caliber intravascular stent of three-layer structure |
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