CN106072414A - A kind of method preparing liquid compound seasoner - Google Patents

A kind of method preparing liquid compound seasoner Download PDF

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Publication number
CN106072414A
CN106072414A CN201610422194.3A CN201610422194A CN106072414A CN 106072414 A CN106072414 A CN 106072414A CN 201610422194 A CN201610422194 A CN 201610422194A CN 106072414 A CN106072414 A CN 106072414A
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China
Prior art keywords
liquid
method preparing
compound seasoner
utilize
fermentation
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Pending
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CN201610422194.3A
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Chinese (zh)
Inventor
张伟
丁昊然
丁智峰
李艺频
蔡春枝
江红
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ANHUI WEIZHIYUAN BIOTECHNOLOGY Co Ltd
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ANHUI WEIZHIYUAN BIOTECHNOLOGY Co Ltd
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Priority to CN201610422194.3A priority Critical patent/CN106072414A/en
Publication of CN106072414A publication Critical patent/CN106072414A/en
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Abstract

The present invention relates to a kind of method preparing liquid compound seasoner, utilize soybean, bean dregs and yellow seriflux as raw material, decomposed by biology enzyme, prepare enzymolysis liquid A, utilizing enzymolysis liquid A to carry out fermentation tank culture medium to prepare, recycling edible fungus species carries out fermentor liquid fermentation, and the mycelium of generation is decomposed by biology enzyme again, the enzymolysis liquid obtaining after filtering clarification again dispensing carry out Maillard reaction, then feed liquid secondary after reaction is compounded.A kind of liquid compound seasoner rich in soybean and edible mushroom elite prepared by said method, flavour is delicious, nutritious, and make use of the byproduct bean dregs that bean product process and yellow seriflux as raw material, has saved production cost.

Description

A kind of method preparing liquid compound seasoner
Technical field
The present invention relates to flavoring production technical field, particularly relate to a kind of method preparing liquid compound seasoner.
Background technology
Compound seasoner is broadly divided into powdered compound seasoning and liquid compound seasoner.Although powdered compound seasoning energy Effectively adjust and improve quality and the local flavor of food, but it exists some defects: as being difficult to sterilizing, sanitary condition difference, cooking Tune affects sense organ and the mouthfeel etc. of dish.And liquid condiment is based on leaching liquor, with bases such as soy sauce, vinegar, cooking wine Plinth flavoring is auxiliary material, is made through certain processing technology with suitable proportioning combination.Liquid compound seasoner is easy to go out Bacterium, safe and sanitary, do not interfere with sense organ and the mouthfeel of dish in the cooking yet, not only compensate for powdered compound seasoning not Foot, and merged multiple basis local flavor of flavoring and nutrition, flavour is delicious, mellow, give off a strong fragrance, and more can give dish bright Beautiful color and luster.
Traditional liquid condiment mainly compounds with soy sauce and monosodium glutamate and various food additives, and production cost is higher, battalion Support and be worth not quite.
Content of the invention
For the problem of shortcomings and deficiencies in prior art, the present invention proposes a kind of side preparing liquid compound seasoner Method, a kind of liquid compound seasoner rich in soybean and edible mushroom elite of preparation, flavour is delicious, nutritious, and make use of The byproduct bean dregs of bean product processing and yellow seriflux, as raw material, have saved production cost.
The technical solution adopted for the present invention to solve the technical problems is:
A kind of method preparing liquid compound seasoner, comprises the steps:
Step one: utilize soybean, bean dregs and yellow seriflux as raw material, decomposed by biology enzyme, prepare enzymolysis liquid A;
Step 2: utilize enzymolysis liquid A to carry out fermentation tank culture medium and prepare;
Step 3: utilize edible fungus species to carry out fermentor liquid fermentation;
Step 4: the mycelium that fermentation produces is decomposed by biology enzyme again;
Step 5: the enzymolysis liquid obtaining through filter clarification after again dispensing carry out Maillard reaction;
Step 6: again feed liquid secondary after reaction is compounded.
Further, the culture medium preparation in described step 2 also includes that first class seed pot culture medium is prepared and secondary seed Prepared by tank culture medium.
Further, the fermentation in described step 3 is to utilize edible mushroom secondary seed bacterium solution, is inoculated by sterile working To fermentation tank.
Further, in described step 6 secondary compound in feed liquid, add 16% edible salt, 2% monosodium glutamate, 0.05% Lactic acid, 0.05% malic acid, the citric acid of 0.1, stirring and dissolving.
Further, described secondary seed bacterium solution is to utilize edible mushroom first order seed bacterium solution, is seeded to by sterile working Secondary seed tank ferments.
Further, described first order seed bacterium solution is to utilize edible mushroom bacterium solution, is seeded to first order seed by sterile working Tank ferments.
There is advantages that and the present invention utilizes soybean, bean dregs and yellow seriflux as raw material, make full use of The byproduct bean dregs of bean product processing and yellow seriflux, saved production cost.In the present invention, flavoring flavour is delicious, nutrition is rich Richness, can substitute the flavouring such as soy sauce, chickens' extract completely.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is expanded on further.Should be understood that these embodiments are merely to illustrate the present invention Rather than restriction the scope of the present invention.In addition, it is to be understood that after having read the content that the present invention lectures, people in the art The present invention can be made various changes or modifications by member, and these equivalent form of values fall within the application appended claims equally and limited Scope.
A kind of method preparing liquid compound seasoner, comprises the steps:
Step one: utilize soybean, bean dregs and yellow seriflux as raw material, decomposed by biology enzyme, prepare enzymolysis liquid A;
Step 2: utilize enzymolysis liquid A to carry out fermentation tank culture medium and prepare;
Step 3: utilize edible fungus species to carry out fermentor liquid fermentation;
Step 4: the mycelium that fermentation produces is decomposed by biology enzyme again;
Step 5: the enzymolysis liquid obtaining through filter clarification after again dispensing carry out Maillard reaction;
Step 6: again feed liquid secondary after reaction is compounded.
Concrete, described liquid complex flavor preparation method for material is as follows in detail:
1. prepared by enzymolysis liquid A
The dry soybean of 1000kg, can produce the wet bean dregs of 2000kg (water content 80%) and 3000kg yellow seriflux, simultaneously with soak wet greatly Beans 200kg adds the direct defibrination of yellow seriflux 800kg, then by the raw beans paste slag of milled with boiling, after mixing three, uses high pressure Homogenizer carries out ultramicro grinding;Slurries stainless steel pump after pulverizing pumps in 10m enzymatic vessel, and insulation, to 45 DEG C, opens stirring, Add neutral proteinase (enzyme activity unit >=50,000 U/g), neutral lipase (enzyme activity unit >=100,000 U/ of 0.05% of 0.1% G), the cellulase (enzyme activity unit >=100,000 U/g) of 0.15%, is kept stirring for speed 60r/min, digests 10 hours;Then use Food-grade concentrated hydrochloric acid adjusts enzymolysis liquid PH=3.0, and stirring is warming up to 50 DEG C, adds 0.05% pectase (enzyme activity unit >=100,000 U/ G) He 0.05% acid protease (enzyme activity unit >=100,000 U/g), is kept stirring for speed 60r/min, standby after digesting 8 hours.
2. prepared by culture medium
Prepared by 2.1 60L first class seed pot culture mediums
Fresh potato 6kg, after soaking and washing, with potato peeler decortication, then wears into raw-soil soya-bean milk with fiberizer, goes to 200L First class seed pot, adds enzymolysis liquid A 12kg, yeast extract 0.3kg, glucose 0.6kg, adds water and is settled to 57L stirring all Even, add 10mol/L NaOH flavouring liquid PH=6.0, standby to feed liquid 121 DEG C sterilizing 30min afterwards.
Prepared by 2.2 1200L secondary seed tank culture mediums
Fresh potato 120kg, after soaking and washing, with potato peeler decortication, then wears into raw-soil soya-bean milk with fiberizer, turns
To 2000L secondary seed tank, add enzymolysis liquid A 240kg, yeast extract 6kg, glucose 12kg, add water and be settled to 1140L stirs, and adds 10mol/L NaOH flavouring liquid PH=6.0, standby to feed liquid 121 DEG C sterilizing 30min afterwards.
Prepared by 2.3 fermentation tank culture medium
Fresh potato 1200kg, after soaking and washing, with potato peeler decortication, then wears into raw-soil soya-bean milk with fiberizer, with not Rust steel pump pumps in 20m fermentation tank, adds enzymolysis liquid A 4800kg, glucose 120kg, adds water and be settled to 10.8
M, adds 10mol/L NaOH flavouring liquid PH=6.0, standby to feed liquid 121 DEG C sterilizing 30min afterwards.
3. edible fungus species fermented and cultured
3.1 first order seeds are cultivated
The 3L edible mushroom bacterium solution preparing laboratory, is seeded to first class seed pot by sterile working, opens stirring and keeps rotating speed 120r/min, from being passed through aseptic compressed air at the bottom of tank, flow is maintained at 1.8m/h, pressure 0.05MP in tank, keeps feed temperature 25 DEG C, cultivate 24 hours.
3.2 secondary seeds are cultivated
It by cultured 60L edible mushroom first order seed bacterium solution, is seeded to secondary seed tank by sterile working, open stirring and keep turning Speed 120r/min, from being passed through aseptic compressed air at the bottom of tank, flow is maintained at 36m/h, pressure 0.05MP in tank, keeps feed liquid temperature Spend 25 DEG C, cultivate 24 hours.
3.3 fermented and cultured
It by cultured 1200L edible mushroom secondary seed bacterium solution, is seeded to fermentation tank by sterile working, open stirring and keep rotating speed 120r/min, from being passed through aseptic compressed air at the bottom of tank, flow is maintained at 360m/h, pressure 0.05MP in tank, keeps feed temperature 25 DEG C, cultivate 72 hours.
4. edible mushroom mycelium biology enzyme decomposes
After fermented and cultured terminates, in fermentation tank, cover with spherical edible mushroom mycelium, now stop being passed through compressed air, be kept stirring for Rotating speed 120r/min, adds 10mol/L NaOH flavouring liquid PH=6.0, then fermented feed liquid is warming up to 50 DEG C, adds 0.1% Neutral proteinase, the dimension element enzyme of 0.05%, digest 4h, and now mycelium is completely degraded into soluble small molecular.
5. filter clarification, dispensing, Maillard reaction
After enzymolysis terminates, by feed liquid plate and frame filter press 500 mesh filter-cloth filtering, the clear liquid after filtration goes to maillard reactions tank In, in the ratio of 10%, add the HFCS of solid content 71%, be warming up to 95 DEG C, open stirring and be kept stirring for rotating speed 60r/min, is incubated 1 hour, makes feed liquid generation maillard reactions, produce strong mushroom fragrance.
6. compound for two times
After Maillard reaction terminates, in feed liquid add 16% edible salt, 2% monosodium glutamate, 0.05% lactic acid, the apple of 0.05% Acid, the citric acid of 0.1, i.e. can be made into after stirring and dissolving rich in amino acid, small peptide, edible fungi polysaccharide, water-soluble dietary fiber, big The liquid compound seasoner of the materials such as beans polysaccharide, lecithin, isoflavones, this product flavour is delicious, nutritious, can replace completely For the flavouring such as soy sauce, chickens' extract.

Claims (6)

1. the method preparing liquid compound seasoner, it is characterised in that comprise the steps:
Step one: utilize soybean, bean dregs and yellow seriflux as raw material, decomposed by biology enzyme, prepare enzymolysis liquid A;
Step 2: utilize enzymolysis liquid A to carry out fermentation tank culture medium and prepare;
Step 3: utilize edible fungus species to carry out fermentor liquid fermentation;
Step 4: the mycelium that fermentation produces is decomposed by biology enzyme again;
Step 5: the enzymolysis liquid obtaining through filter clarification after again dispensing carry out Maillard reaction;
Step 6: again feed liquid secondary after reaction is compounded.
2. a kind of method preparing liquid compound seasoner according to claim 1, it is characterised in that: in described step 2 Culture medium preparation also include first class seed pot culture medium preparation and secondary seed tank culture medium prepare.
3. a kind of method preparing liquid compound seasoner according to claim 1, it is characterised in that: in described step 3 Fermentation be to utilize edible mushroom secondary seed bacterium solution, be seeded to fermentation tank by sterile working.
4. a kind of method preparing liquid compound seasoner according to claim 1, it is characterised in that: in described step 6 Secondary compound in feed liquid add 16% edible salt, 2% monosodium glutamate, 0.05% lactic acid, 0.05% malic acid, the lemon of 0.1 Acid, stirring and dissolving.
5. a kind of method preparing liquid compound seasoner according to claim 3, it is characterised in that: described secondary seed Bacterium solution is to utilize edible mushroom first order seed bacterium solution, is seeded to secondary seed tank by sterile working and ferments.
6. a kind of method preparing liquid compound seasoner according to claim 4, it is characterised in that: described first order seed Bacterium solution is to utilize edible mushroom bacterium solution, is seeded to first class seed pot by sterile working and ferments.
CN201610422194.3A 2016-06-15 2016-06-15 A kind of method preparing liquid compound seasoner Pending CN106072414A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108410782A (en) * 2018-05-25 2018-08-17 南京工业大学 Fermentation medium containing waste bean curd yellow serofluid and waste bean dregs and application thereof
CN112471489A (en) * 2020-11-12 2021-03-12 内蒙古中谷君创生物科技发展有限责任公司 Low-fat nutritional salad sauce prepared from mycelium of Dictyophora Indusiata (Vent. Ex pers) Fisch and Polyporus tiger-palm and its preparation method
CN114431448A (en) * 2022-02-23 2022-05-06 南京高新工大生物技术研究院有限公司 Seasoning based on edible fungus fermentation technology and preparation method thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102178205A (en) * 2011-06-02 2011-09-14 成都味源生物工程科技有限公司 Method for preparing lactalbumin hydrolysate without chloropropanol by utilizing soybean meal
CN102318864A (en) * 2011-07-27 2012-01-18 武汉百安生物科技有限责任公司 Drink containing soy peptide and edible fungi polysaccharide and preparation method thereof
CN105265910A (en) * 2014-07-18 2016-01-27 广州市味研生物工程科技有限公司 Method for preparing taste-active peptide through fermentation of composite probiotics

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102178205A (en) * 2011-06-02 2011-09-14 成都味源生物工程科技有限公司 Method for preparing lactalbumin hydrolysate without chloropropanol by utilizing soybean meal
CN102318864A (en) * 2011-07-27 2012-01-18 武汉百安生物科技有限责任公司 Drink containing soy peptide and edible fungi polysaccharide and preparation method thereof
CN105265910A (en) * 2014-07-18 2016-01-27 广州市味研生物工程科技有限公司 Method for preparing taste-active peptide through fermentation of composite probiotics

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108410782A (en) * 2018-05-25 2018-08-17 南京工业大学 Fermentation medium containing waste bean curd yellow serofluid and waste bean dregs and application thereof
CN108410782B (en) * 2018-05-25 2022-04-22 南京工业大学 Fermentation medium containing waste bean curd yellow serofluid and waste bean dregs and application thereof
CN112471489A (en) * 2020-11-12 2021-03-12 内蒙古中谷君创生物科技发展有限责任公司 Low-fat nutritional salad sauce prepared from mycelium of Dictyophora Indusiata (Vent. Ex pers) Fisch and Polyporus tiger-palm and its preparation method
CN114431448A (en) * 2022-02-23 2022-05-06 南京高新工大生物技术研究院有限公司 Seasoning based on edible fungus fermentation technology and preparation method thereof
CN114431448B (en) * 2022-02-23 2023-07-25 南京高新工大生物技术研究院有限公司 Flavoring based on edible fungus fermentation technology and preparation method thereof

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Application publication date: 20161109