CN106071524B - A kind of asparagus fermented beverage preparation method with nutrition health-care functions - Google Patents

A kind of asparagus fermented beverage preparation method with nutrition health-care functions Download PDF

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Publication number
CN106071524B
CN106071524B CN201610403989.XA CN201610403989A CN106071524B CN 106071524 B CN106071524 B CN 106071524B CN 201610403989 A CN201610403989 A CN 201610403989A CN 106071524 B CN106071524 B CN 106071524B
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asparagus
lactobacillus paracasei
cicc
fermented beverage
beverage preparation
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CN106071524A (en
Inventor
程池
陈建国
梁寒峭
张奇
刘洋
李生有
尹俊玉
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Bei Jing Xnk Research Centre Of Asparagus
China National Research Institute of Food and Fermentation Industries
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Bei Jing Xnk Research Centre Of Asparagus
China National Research Institute of Food and Fermentation Industries
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38Other non-alcoholic beverages
    • A23L2/382Other non-alcoholic beverages fermented
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/165Paracasei

Abstract

The asparagus fermented beverage preparation method with nutrition health-care functions that the invention discloses a kind of, belongs to fermented type drinks manufacture field.From healthy asparagus isolated lactobacillus paracasei CICC6270 and lactobacillus paracasei CICC6271, it is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number is CGMCC NO.12211 and CGMCC NO.12212.It using the full bamboo shoot of asparagus as raw material, is fermented using this two strains of lactic acid bacteria, has been obviously improved asparagus active constituent saponin(e, flavones and polysaccharide dissolution rate, obtain novel asparagus fermented beverage.Compared with fresh squeezing asparagus juice, asparagus saponin(e, flavones and polysaccharide dissolution rate is respectively increased to 8.4 times, 2.5 times and 2.9 times in the method for the present invention.The asparagus fermented beverage of this technique preparation has the health-care effects such as reducing blood lipid, blood pressure lowering, strengthen immunity.

Description

A kind of asparagus fermented beverage preparation method with nutrition health-care functions
Technical field
The asparagus fermented beverage preparation method with nutrition health-care functions that the present invention relates to a kind of belongs to fermented type function drink Expect manufacture field.
Background technique
Asparagus (Asparagus officinalis Linn.) also known as asparagus, asparagus, enjoy the beauty of " kings of vegetables " Claim.Shennong's Herbal is recorded: " asparagus person, asparagus.The king of hundred vegetables, on top grade.Long term usage, QI invigorating of making light of one's life by commiting suicide macrobiosis "." this Careless detailed outline " it records: " asparagus person, energy goitre knot hot gas, diuresis, moistening lung antibechic, eliminating the phlegm, desinsection." modern scientific research shows Asparagus not only contains the microelements such as multivitamin and amino acid and selenium, molybdenum, manganese, but also the active constituent rich in there are many, such as Asparagus Flavonoids, saponin(e, polysaccharose substance have the medicines such as reducing blood lipid, hypoglycemic and blood pressure lowering, strengthen immunity, anti-oxidant, anticancer Reason effect, often feeding play the role of it is antifatigue, promote longevity.
Though new fresh asparagus is rich in nutrition and active material, institutional framework is fine and close, and human body is raw by the way of directly eating Object availability is low.Asparagus fermented product is primarily present that mouthfeel is bad, and raw material availability is low at present, and selection limitation is larger, and makes It is baked in standby step using long-time solarization or high temperature, the disadvantages of larger is destroyed to asparagus nutrition and active constituent.
There is the food formula using inoculating microbe fermented asparagus in the patent document in China, such as 104012945 A of CN With 103431467 B of CN, all lactic acid bacteria only with external source addition or saccharomycete, these microorganisms are without specific metabolism Product and its health-care effect, and the distinctive nutrition of asparagus and active constituent can be destroyed, and there are Germinatus Phragmitis extracts at high cost, fermentation The defects of viable bacteria beverage stability is poor.By the retrieval of above patent document and non-patent literature, there is presently no discoveries to utilize Functional microorganism obtained in healthy asparagus, the improvement natural fermentating process strengthened by endogenous microbes, the tool being prepared The asparagus fermented product of nutritious health-care effect.
Summary of the invention
The present invention provides a kind of asparagus fermented beverage and preparation method thereof with nutrition health-care functions, first zymophyte The functional microorganism obtained in the healthy asparagus kind is selected, metabolic pathway is more suitable for asparagus fermentation, sufficiently release and retains reed Bamboo shoot nutrition and active constituent;Secondly, asparagus raw material is the full bamboo shoot for including bamboo shoot base, abundance, cost are less expensive, avoid extracting and grasp Cause the nutritive loss of raw material;Again, the sweet wormwood taste and bitter taste of asparagus be can remove, hence it is evident that improve mouthfeel, provide a kind of wind Taste uniqueness, asparagus fermented product with health care function.
The present invention provides a kind of asparagus fermented beverage preparation method with nutrition health-care functions, includes the following steps:
(1) from healthy asparagus isolated lactobacillus paracasei (Lactobacillus paracasei) CICC6270 With lactobacillus paracasei (Lactobacillus paracasei) CICC6271;
(2) using new fresh asparagus as raw material, including the tender tips of bamboo shoot, bamboo shoot stem and bamboo shoot base, it cleans plus water is broken and is homogenized, sterilizing;
(3) into asparagus homogenate, it is inoculated with lactobacillus paracasei CICC6270 and lactobacillus paracasei CICC6271, is sent out Ferment is separated by solid-liquid separation, after allotment mouthfeel, obtains asparagus fermented juice;
Lactobacillus paracasei as described in step (1) (Lactobacillus paracasei) CICC6270 and secondary cheese Lactobacillus (Lactobacillus paracasei) CICC6271, the deposit number of the bacterial strain is respectively CGMCC NO.12211 With CGMCC NO.12212, the deposit date is on March 14th, 2016, depositary institution was Chinese microorganism strain preservation conservator Meeting common micro-organisms center, preservation address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3;
The quality proportioning of the control asparagus and the water is 1:0.5~1:5 in step (2), and the asparagus is broken It is broken to 10~20 mesh;
Lactobacillus paracasei CICC6270 described in step (3) and lactobacillus paracasei CICC6271 is trained using basic bacteria Support base (50% asparagus juice of addition and 2% glucose), 37 DEG C of standings, activation culture 16-30 h;The lactobacillus paracasei CICC6270 and lactobacillus paracasei CICC6271 volume proportion are 3:1~3:5, and control fermentation temperature is 15-40 DEG C, when fermentation Between be 12-96 h;
The implementation of the present invention program, at least has the advantage that
According to the method for the present invention, fermenting microbe has selected the function lactic acid bacteria obtained in the healthy asparagus, and metabolic pathway is more It is suitble to asparagus fermentation, sufficiently release and retains asparagus nutrition and active constituent, significantly improves Asparagus Flavonoids class, saponins and more The dissolution rate of carbohydrate active constituent greatly strengthens the health-care effect of asparagus fermented beverage.The asparagus fermented product liquid color It is golden yellow, clear, and asparagus smell and lactic acid fragrance with faint scent.
Specific embodiment
Below in conjunction with specific embodiment, further the present invention is described in detail, so that the purpose of the present invention, technology Scheme and advantage are clearer.Following embodiment is used only for for example, not limiting the present invention in any way.
Embodiment 1
10 g fresh and healthy asparagus bamboo shoot bases are weighed, with aseptic water washing, later successively with 70% ethyl alcohol, liquor natrii hypochloritis (2.5% effective Cl-), 70% ethyl alcohol impregnates 3min, 5min, 30s, then is eluted 6 times with sterile water, and dry under aseptic condition, The purpose of surface sterilizing is realized;The sample of surface sterilizing processing is placed in grind into powder in sterile mortar, is then successively used It is 10 that sterile water, which is diluted to concentration,-1、10-2、10-3Suspension.200 μ L are taken to be coated on the MRS plate of 30% asparagus juice of addition respectively On, each processing three is parallel, is placed in 35 DEG C and cultivates 2-3 days.According to colonial morphology (size, shape, color, surface gloss, Neat in edge degree, transparency etc.), have discrepant representative colonies on random picking plate, is inoculated in after purification opposite 4 DEG C of slant medium are answered to save backup.
After functional screening is tested, fermenting microbe selects the isolated lactobacillus paracasei from healthy asparagus CICC6270 and lactobacillus paracasei CICC6271, the deposit number of the bacterial strain are respectively CGMCC NO.12211 and CGMCC NO.12212。
Fermenting microbe is used to MRS activation culture respectively.Lactobacillus paracasei CICC6270 and lactobacillus paracasei CICC6271 is cultivated using based seed media (50% asparagus juice and 2% glucose), 37 DEG C of 30 h of stationary culture.
Fresh full bamboo shoot surface clean, disinfection are chosen, in 1:2(mass ratio) ratio adds water broken, and it is sieved through and filters through the concussion of 40 mesh It obtains asparagus to starch entirely, using ultra high temperature short time sterilization, condition is 135 DEG C of 15s.Secondary cheese cream bar is cooled to room temperature under rear aseptic condition 1:1 is inoculated with by volume for bacterium CICC6270 and lactobacillus paracasei CICC6271 leavening, total inoculum concentration 5%, 37 DEG C of cultures, when PH stablizes to 3.6, and content of reducing sugar terminates to ferment when being lower than 0.3%, cools to 15 DEG C, after-ripening 3 months.After-ripening is completed, solid-liquid point From fermented juice is obtained, 135 DEG C of sterilizing 15s obtain invention product 1.
Embodiment 2
In embodiment 1, lactobacillus paracasei CICC6270 and lactobacillus paracasei CICC6271 leavening 2:1 by volume Ratio inoculation, total inoculum concentration are 5%, in addition to this progress and the identical operation of embodiment 1, obtain invention product 2.
Embodiment 3
In embodiment 1, lactobacillus paracasei CICC6270 and lactobacillus paracasei CICC6271 leavening 1:1 by volume Ratio inoculation, total inoculum concentration are 3%, in addition to this progress and the identical operation of embodiment 1, obtain invention product 3.
Comparative example 1
In embodiment 1, it is not inoculated with any strain, in addition to this progress and the identical operation of embodiment 1, are compared Product 1.
Comparative example 2-5
In embodiment 1, lactobacillus paracasei CICC6270 and lactobacillus paracasei are replaced using ordinary lactic acid bacteria respectively CICC6271, in addition to this progress and the identical operation of embodiment 1, obtain comparing product 2-5.
Constituent analysis measuring method
General flavone content: aluminum nitrate development process
Total saponin content: perchloric acid development process
Total starches content: phenol-sulphoacid development process
1 invention product 1-3 of table and the measured value for comparing total saposins, general flavone and total starches in product 1-5
Compare product 1 and is not inoculated with strain fermentation.
Compare the invention product except product 1, compare product and be all vaccinated with strain and ferment.
As shown in table 1, asparagus raw material lactobacillus paracasei CICC6270 and lactobacillus paracasei CICC6271 is inoculated with to send out The product 1-3 of the present invention that yeast-like fungi agent obtains is not compared with being inoculated with the comparison product 1 that strain fermentation obtains, total saposins, general flavone and total more The amount of sugar about increases to 8.4 times, 2.5 times and 2.9 times respectively.
As shown in table 1, asparagus raw material lactobacillus paracasei CICC6270 and lactobacillus paracasei CICC6271 is inoculated with to send out The product 1-3 of the present invention that yeast-like fungi agent obtains is total yellow compared with the comparison product 2 that only inoculation lactobacillus paracasei CICC6270 ferments The amount of ketone about increases to 2.1 times.
As shown in table 1, asparagus raw material lactobacillus paracasei CICC6270 and lactobacillus paracasei CICC6271 is inoculated with to send out The product 1-3 of the present invention that yeast-like fungi agent obtains is compared with the comparison product 3 that only inoculation lactobacillus paracasei CICC6271 ferments, total soap The amount of glycosides about increases to 7.0 times.
As shown in table 1, asparagus raw material lactobacillus paracasei CICC6270 and lactobacillus paracasei CICC6271 is inoculated with to send out The product 1-3 of the present invention that yeast-like fungi agent obtains is compared with the comparison product 4-7 that inoculation ordinary lactic acid bacteria kind is fermented, total saposins, total Huang The amount of ketone and total starches about increases to 5.7-17.5 times, 2.1-4.7 times and 1.5-3.1 times respectively.
Combining specific embodiment above, the present invention is described, these specific embodiments are only exemplary , this does not limit the scope of protection of the present invention, and those skilled in the art can be under the premise of not departing from essence of the present invention It carry out various modifications, change or replacement.Therefore, it according to various equivalent variationss made by the present invention, still falls within the present invention and is covered Range.

Claims (6)

1. a kind of asparagus fermented beverage preparation method with nutrition health-care functions, which comprises the steps of:
(1) the isolated lactobacillus paracasei CICC 6270 and lactobacillus paracasei CICC 6271 from healthy asparagus;
(2) using new fresh asparagus as raw material, including the tender tips of bamboo shoot, bamboo shoot stem and bamboo shoot base, it cleans plus water is broken and is homogenized, sterilizing;
(3) into asparagus homogenate, it is inoculated with lactobacillus paracasei CICC 6270 and lactobacillus paracasei CICC 6271, is sent out Ferment is separated by solid-liquid separation, after allotment mouthfeel, obtains novel asparagus fermented beverage.
2. asparagus fermented beverage preparation method according to claim 1, which is characterized in that as described in step (1) secondary dry Lactobacillus paracasei CICC 6270 and lactobacillus paracasei CICC 6271, culture presevation CGMCC NO. 12211 and CGMCC NO. 12212。
3. asparagus fermented beverage preparation method according to claim 1, which is characterized in that the control reed in step (2) The quality proportioning of bamboo shoot and the water is 1:0.5 ~ 1:5, and the asparagus is crushed to 10-20 mesh.
4. asparagus fermented beverage preparation method according to claim 1, which is characterized in that pair cheese described in step (3) Lactobacillus CICC 6270 and lactobacillus paracasei CICC 6271 is using the training of the basic bacteria of 50% asparagus juice of addition and 2% glucose Support base, 37 DEG C of standings, activation culture 16-30 h.
5. asparagus fermented beverage preparation method according to claim 1, which is characterized in that pair cheese described in step (3) Lactobacillus CICC 6270 and 6271 volume proportion of lactobacillus paracasei CICC are 3:1 ~ 3:5, and control fermentation temperature is 15-40 DEG C, fermentation time is 12-96 h.
6. the method according to claim 1, wherein lactobacillus paracasei CICC 6270 and CICC 6271 exist Application in asparagus fermented beverage with nutrition health-care functions.
CN201610403989.XA 2016-06-12 2016-06-12 A kind of asparagus fermented beverage preparation method with nutrition health-care functions Active CN106071524B (en)

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Publication number Priority date Publication date Assignee Title
CN107865352A (en) * 2017-11-08 2018-04-03 苏州健世星生物科技有限公司 A kind of preparation method of high nutrition asparagus healthy food
CN109832618A (en) * 2019-01-04 2019-06-04 中国食品发酵工业研究院有限公司 A kind of lily fermented product and preparation method thereof
CN110150523A (en) * 2019-06-14 2019-08-23 扬州大学 A kind of novel fermentation asparagus beverage preparation method
CN111450260B (en) * 2020-04-17 2021-06-25 西南交通大学 Bifidobacterium bifidum anti-tumor preparation, and preparation method, product and application thereof

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Publication number Priority date Publication date Assignee Title
JP2002027955A (en) * 2000-07-17 2002-01-29 Asahi Soft Drinks Co Ltd Drink of fermented vegetable such as cabbage
JP4628978B2 (en) * 2006-03-08 2011-02-09 ユニチカ株式会社 Method for producing composition with high content of γ-aminobutyric acid
CN102008055A (en) * 2010-12-21 2011-04-13 南昌大学 Fruit and vegetable jam and preparation method thereof
CN102885343A (en) * 2011-07-19 2013-01-23 江西食方食坊中药食品有限公司 Anticancer health asparagus beverage and preparation method thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002027955A (en) * 2000-07-17 2002-01-29 Asahi Soft Drinks Co Ltd Drink of fermented vegetable such as cabbage
JP4628978B2 (en) * 2006-03-08 2011-02-09 ユニチカ株式会社 Method for producing composition with high content of γ-aminobutyric acid
CN102008055A (en) * 2010-12-21 2011-04-13 南昌大学 Fruit and vegetable jam and preparation method thereof
CN102885343A (en) * 2011-07-19 2013-01-23 江西食方食坊中药食品有限公司 Anticancer health asparagus beverage and preparation method thereof

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