CN106071436A - A kind of Germinatus Phragmitis fermented beverage that can improve sleep quality and preparation method thereof - Google Patents
A kind of Germinatus Phragmitis fermented beverage that can improve sleep quality and preparation method thereof Download PDFInfo
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- CN106071436A CN106071436A CN201610406063.6A CN201610406063A CN106071436A CN 106071436 A CN106071436 A CN 106071436A CN 201610406063 A CN201610406063 A CN 201610406063A CN 106071436 A CN106071436 A CN 106071436A
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- germinatus phragmitis
- lactobacillus paracasei
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- 230000004151 fermentation Effects 0.000 claims abstract description 28
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims abstract description 26
- BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 claims abstract description 25
- 241000186605 Lactobacillus paracasei Species 0.000 claims abstract description 24
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/02—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of Germinatus Phragmitis fermented beverage that can improve sleep quality and preparation method thereof, belong to fermented type drinks manufacture field.With the full Radix Crotalariae szemoensis of Germinatus Phragmitis as raw material, utilize Lactobacillus paracasei CICC6270 and Lactobacillus paracasei CICC6271 to ferment, be obviously improved the dissolution rate of Germinatus Phragmitis saponin component, obtain Germinatus Phragmitis fermentation natural juice.Compared with fresh squeezing asparagus juice, the inventive method makes Germinatus Phragmitis saponin component dissolution rate improve to 8.28 times.Using this Germinatus Phragmitis fermentation natural juice is primary raw material, adds gamma aminobutyric acid, and the compositions of Semen Ziziphi Spinosae extract, Radix Ginseng extract, Fructus Schisandrae Chinensis extrat, Synergistic each other, significantly enhances the improvement sleep effect of Germinatus Phragmitis fermented product.The present invention prepares novel Germinatus Phragmitis Fermentation Function beverage, it is possible to be effectively improved sleep quality, pure natural, has no side effect, can be as the drug substitute improving sleep.
Description
Technical field
The present invention relates to a kind of Germinatus Phragmitis fermented beverage that can improve sleep quality and preparation method thereof, belong to fermented type merit
Can beverage production field.
Background technology
Sleep is the psychological need that body weight for humans is wanted, be body restore, integrate and the important step of consolidating memory, be health not
The ingredient that can lack.Investigating according to World Health Organization (WHO), the people of 27% has sleeping problems.Insomnia be common clinical disease it
One, sickness rate is the highest, and life and social mentality and physiological function etc. to people all have very serious implications.Obstinate mistake
Sleep, bring long-term misery to patient.At present, mainly select hypnotic treatment insomnia, as stable, as life-time service is pacified
Dormancy medicine, it will dependency raw to sleeping pill produce, will seriously damage hepatic and/or renal function simultaneously.
Germinatus Phragmitis (Asparagus officinalis Linn.) have another name called asparagus, Thallus Gracilariae, enjoy the U.S. of " king of vegetable "
Claim.Shennong's Herbal is recorded: " Germinatus Phragmitis person, asparagus is also.The king of hundred vegetables, top grade on.Clothes for a long time, QI invigorating of making light of one's life by commiting suicide is prolonged life ".Reed
Radix Crotalariae szemoensis do not contain only the trace element such as multivitamin and aminoacid and selenium, molybdenum, manganese, and rich in there being various active composition, often eat
There is the effect of resisting fatigue, life lengthening.Natural plants saponin belongs to steroid compound, has good to insomnia and neurasthenia
Good therapeutic effect, such as ginsenoside, jujuboside etc..And Germinatus Phragmitis saponin is one of Germinatus Phragmitis main active, have equally
Have calm the nerves, calm and improve the effect of sleep.Zoopery shows the instant asparagus powder powder improvement result to mice sleep, has
Work in coordination with pentobarbital sodium sub-threshold dose syngignoscism, can significantly shorten pentobarbital sodium Sleep latency, notable prolongation penta bar ratio
Appropriate sodium length of one's sleep.
Semen Ziziphi Spinosae: jujube nucleus, calls Semen Ziziphi Spinosae, Fructus Choerospondiatis." Chinese Pharmacopoeia " record Semen Ziziphi Spinosae have sour in the mouth put down, return liver, gallbladder,
Heart channel, is mainly used in treating restlessness of asrhenia type and insomnia, dreaminess of palpitating with fear, body void hyperhidrosis, and Tianjin wound is thirsty.Semen Ziziphi Spinosae has calmness, hypnosis, town
Bitterly, the pharmacological action such as convulsion, blood pressure lowering, blood fat reducing.Animal experiment study finds: Semen Ziziphi Spinosae water extract can be obviously prolonged penta bar ratio
The length of one's sleep of the mice of appropriate sodium induction.
Radix Ginseng is China's tradition rare Chinese medicine, has supplementary vigour, the mind calming and mentality promoting, invigorating the spleen and benefiting QI, the effect of promoting the production of body fluid etc..Radix Ginseng
Saponin can significantly extend mouse survival time and swimming time, shows that Radix Ginseng has antifatigue effect.Ginsenoside can reduce little
Mus is brain and the lactic acid content of cardiac muscle in the case of severe depletion of oxygen, can reduce the ratio of cardiac muscle CANP '/CGMP when recovering anoxia,
Show that ginsenoside can improve energy metabolism when being organized in anoxia.
Fructus Schisandrae Chinensis (Chinese magnoliavine fruit) is perennial fallen leaves liana, belongs to Magnoliaceae." herbal classic " is remembered
Carry Fructus Schisandrae Chinensis " main QI invigorating, tonifying for the deficiency, reinforcing YIN-essence, benefit man's essence ".Schisandra active component lignanoids, predominantly schizandrin
(schizandrin) and the like, there is obvious sedation.Mouse experiment represents that Chinese Magnoliavine Fruit alcohol extract can significantly reduce
The autonomic activities of mice, extends the mouse sleep time that mice barbital sodium and pentobarbital sodium cause, antagonism galvanic shock, penta 4
The convulsions that azoles etc. cause, and there is analgesia and myorelaxant effects.Fructus Schisandrae Chinensis extrat also has antioxidation, can purged body
Interior free radical and the formation of suppression lipid peroxide.
γ-aminobutyric acid (being called for short GABA) is a kind of naturally occurring nonprotein amino acid, is mammalian central
Inhibitory nerve important in nervous system passes on material, and the nervus centralis synapses of about 50% is with GABA as mediator.People
Body cerebral cortex, Hippocampus, thalamus, ganglion basal and cerebellum play an important role, have calm the nerves, calm, lowering blood pressure and blood fat
Etc. physiological function.When GABA lacks in human body, anxiety, uneasiness, the emotion such as tired, worried can be produced, be in height the most for a long time
Pressure group is such as in the crowd in competitive environment, athlete, working clan etc., and being all easy to lack GABA needs to supplement in time
So as relieving mood.
The food formula utilizing common micro-organisms fermented asparagus is had, such as CN 104012945 A in the patent documentation of China
With CN 103431467 B, all only with the common lactic acid bacteria added or yeast, these microorganisms are without clear and definite metabolism
Product and health-care effect thereof, and the distinctive nutrition of Germinatus Phragmitis and active component can be destroyed, and there is Germinatus Phragmitis extract cost height, fermentation
The defects such as viable bacteria beverage stability difference.By above-mentioned patent documentation and the retrieval of non-patent literature, there is presently no discovery and utilize
Endogenous microbes forced fermentation Germinatus Phragmitis raw material, with the Chinese herbal medicine of novel Germinatus Phragmitis fermented juice and integration of edible and medicinal herbs as primary raw material, preparation
There is the Germinatus Phragmitis Fermentation Function product improving sleep quality.
Summary of the invention
The invention provides a kind of Germinatus Phragmitis fermented beverage that can improve sleep quality and preparation method thereof, first zymocyte
Planting and selected the functional microorganism obtained from healthy Germinatus Phragmitis, metabolic pathway is more suitable for Germinatus Phragmitis fermentation, substantially increases Germinatus Phragmitis many
Sugar, saponin and the dissolution rate of flavone, significantly improve absorption and the utilization of Germinatus Phragmitis active component；Secondly, γ-aminobutyric acid is added
And have calm the nerves, calm and improve the integration of edible and medicinal herbs such as the Semen Ziziphi Spinosae extract of sleep effect, Radix Ginseng extract, Fructus Schisandrae Chinensis extrat
Compositions, each other Synergistic, greatly strengthen the health-care effect of Germinatus Phragmitis fermented product.
The present invention provides a kind of Germinatus Phragmitis fermented beverage that can improve sleep quality and preparation method thereof, including walking as follows
Rapid:
(1) with new fresh asparagus as raw material, inoculation Lactobacillus paracasei (Lactobacillus paracasei) CICC6270 and pair
Lactobacillus casei (Lactobacillus paracasei) CICC6271, to ferment, solid-liquid separation obtains novel Germinatus Phragmitis proferment
Juice；
(2) in Germinatus Phragmitis fermentation natural juice, γ-aminobutyric acid, Semen Ziziphi Spinosae extract, Radix Ginseng extract, Fructus Schisandrae Chinensis extrat are added
Etc. compositions, prepare novel Germinatus Phragmitis Fermentation Function beverage；
Lactobacillus paracasei described in step (1) (Lactobacillus paracasei) CICC6270 and secondary cheese
Lactobacillus (Lactobacillus paracasei) CICC6271, the patent bacterium preserving number of described bacterial strain is respectively CGMCC
NO.12211 and CGMCC NO.12212, preservation date is on March 14th, 2016, and depositary institution is Chinese microorganism strain preservation
Administration committee's common micro-organisms center, preservation address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3；
Lactobacillus paracasei CICC6270 described in step (1) and Lactobacillus paracasei CICC6271 volume proportion are 3:1
～3:5, controlling fermentation temperature is 15～40 DEG C, and fermentation time is 12～96 h；Described Lactobacillus paracasei CICC6270 and pair
Lactobacillus casei CICC6271 uses based seed media (adding 50% asparagus juice and 2% glucose) 37 DEG C to stand activation 30
h。
γ-aminobutyric acid, Semen Ziziphi Spinosae extract, Radix Ginseng extract, Fructus Schisandrae Chinensis extrat and reed described in step (2)
The quality percent by volume of Radix Crotalariae szemoensis fermentation natural juice respectively be 0.05～0.2 %, 0.02～0.4 %, 0.015～0.3%, 0.03
～0.25%；
Proving through animal function test, product of the present invention has auxiliary and improves the health-care effect of sleep.Product of the present invention
Amount and eating method be: as a example by the product of 50 mL/ bottle specifications, every day 2 bottles, the most each 1 bottle.
The enforcement of the present invention program, at least has the advantage that
Select the function lactic acid bacteria obtained from healthy Germinatus Phragmitis according to the inventive method, first fermented bacterium, fully released
Put and retain Germinatus Phragmitis nutrition and active component, significantly improving Germinatus Phragmitis polysaccharide, saponins and the dissolution rate of flavonoid active component,
Significantly improve absorption and the utilization of Germinatus Phragmitis active component；Secondly, add have calm the nerves, calm and improve the γ of sleep effect-
The compositionss such as aminobutyric acid, Semen Ziziphi Spinosae extract, Radix Ginseng extract, Fructus Schisandrae Chinensis extrat, Synergistic each other, increases
The strong health-care effect of Germinatus Phragmitis fermented product.
Detailed description of the invention
Come further below in conjunction with specific embodiment that the present invention is described in detail, so that the purpose of the present invention, technology
Scheme and advantage are clearer.Following example are used only for illustrating, and the present invention does not constitute any restriction.
Embodiment 1
Weighing 10 g fresh and healthy Germinatus Phragmitis Radix Crotalariae szemoensis bases, with aseptic water washing, use 70% ethanol the most successively, liquor natrii hypochloritis (2.5%
Effectively Cl-), 70% soak with ethanol 3min, 5min, 30s, then with sterilized water drip washing 6 times, and dry under aseptic condition, realize
The purpose of surface sterilizing；The sample that surface sterilizing processes is placed in grind into powder in sterile mortar, uses sterilized water the most successively
Being diluted to concentration is 10-1、10-2、10-3Suspension.Take 200 μ L respectively and coat on the MRS flat board adding 30% asparagus juice, each
Process three parallel, be placed in 35 DEG C cultivate 2-3 days.According to colonial morphology, (size, shape, color, surface gloss, edge are whole
Qi Du, transparency etc.), random picking flat board has discrepant representative colonies, is inoculated in corresponding inclined-plane after purification
Culture medium 4 DEG C saves backup.
After functional screening is tested, fermented bacterium selects isolated Lactobacillus paracasei from healthy Germinatus Phragmitis
CICC6270 and Lactobacillus paracasei CICC6271, the preserving number of described bacterial strain is respectively CGMCC NO.12211 and CGMCC
NO.12212。
Preparation method:
(1) first Lactobacillus paracasei CICC6270 and Lactobacillus paracasei CICC6271 is used MRS activation culture respectively, then
Cultivate with based seed media (50% asparagus juice and 2% glucose), 37 DEG C of quiescent culture 20 h.Choose fresh full Radix Crotalariae szemoensis surface clear
Wash, sterilize, in 1:2(mass ratio) ratio adds water broken, through 10 mesh concussions be sieved through filter Germinatus Phragmitis is starched entirely, sterilizing, condition is 105 DEG C
15min.After being cooled to room temperature, CICC6270 and CICC6271 1:1 by volume inoculates, total inoculum concentration 3 %, 37 DEG C of cultivations, when
PH is stable to 3.6, terminates fermentation when content of reducing sugar is less than 0.3%, and solid-liquid separation obtains Germinatus Phragmitis fermentation natural juice.
(2) the Germinatus Phragmitis fermentation natural juice prepared by step (1) and the comparison of fresh squeezing asparagus juice total saponins constituents content, assay method
Using perchloric acid development process, measurement result is as follows:
Table 1 Germinatus Phragmitis fermentation natural juice and the comparison of fresh squeezing asparagus juice total saponin content
As shown in table 1, compared with fresh squeezing asparagus juice, Germinatus Phragmitis fermentation natural juice total saponin content increases to 8.28 times.
(3) Germinatus Phragmitis fermentation natural juice 100 L
γ-aminobutyric acid 50 g
Semen Ziziphi Spinosae extract 200 g
Radix Ginseng extract 80 g
Fructus Schisandrae Chinensis extrat 70 g
It is prepared from by above raw material, then through ultra high temperature short time sterilization, obtains invention product 1.
Embodiment 2
Preparation method:
(1) concrete steps are with (1) in embodiment 1.
(2) Germinatus Phragmitis fermentation natural juice 100 L；
γ-aminobutyric acid 80 g；
Semen Ziziphi Spinosae extract 100 g；
Radix Ginseng extract 50 g；
Fructus Schisandrae Chinensis extrat 100 g；
It is prepared from by above raw material, then through ultra high temperature short time sterilization, obtains invention product 2.
Embodiment 3
Preparation method:
(1) first Lactobacillus paracasei CICC6270 and Lactobacillus paracasei CICC6271 is used MRS activation culture respectively, then
Cultivate with based seed media (30% asparagus juice and 1% glucose), 37 DEG C of quiescent culture 30 h.Choose fresh full Radix Crotalariae szemoensis surface clear
Wash, sterilize, in 1:3(mass ratio) ratio adds water broken, through 6 mesh concussions be sieved through filter Germinatus Phragmitis is starched entirely, sterilizing, condition is 100 DEG C
20 min.After being cooled to room temperature, CICC6270 and CICC6271 1:1 by volume inoculates, total inoculum concentration 2 %, 37 DEG C of cultivations, when
PH is stable to 3.8, terminates fermentation, solid-liquid separation when content of reducing sugar is less than 0.3%, obtains Germinatus Phragmitis fermentation natural juice.
(2) Germinatus Phragmitis fermentation natural juice 100 L；
γ-aminobutyric acid 80 g；
Semen Ziziphi Spinosae extract 80 g；
Radix Ginseng extract 80 g；
Fructus Schisandrae Chinensis extrat 90 g；
It is prepared from by above raw material, then through ultra high temperature short time sterilization, obtains invention product 3.
Describing the present invention above in association with detailed description of the invention, these detailed description of the invention are merely exemplary, no
Can limit protection scope of the present invention with this, those skilled in the art can be carried out respectively on the premise of without departing from essence of the present invention
Plant amendment, change or replace.Therefore, the various equivalent variations made according to the present invention, still fall within the scope that the present invention is contained.
Claims (6)
1. Germinatus Phragmitis fermented beverage that can improve sleep quality and preparation method thereof, it is characterised in that comprise the steps:
(1) with new fresh asparagus as raw material, inoculation Lactobacillus paracasei CICC6270 and Lactobacillus paracasei CICC6271 carries out sending out
Ferment, solid-liquid separation obtains novel Germinatus Phragmitis fermentation natural juice；
(2) in Germinatus Phragmitis fermentation natural juice, γ-aminobutyric acid, Semen Ziziphi Spinosae extract, Radix Ginseng extract, Fructus Schisandrae Chinensis extrat are added
Etc. compositions, prepare novel Germinatus Phragmitis Fermentation Function beverage.
Germinatus Phragmitis fermented beverage the most according to claim 1 and preparation method thereof, it is characterised in that described in step (1)
Lactobacillus paracasei CICC6270 and Lactobacillus paracasei CICC6271, patent culture presevation number be CGMCC NO.12211 and
CGMCC NO.12212。
Germinatus Phragmitis fermented beverage the most according to claim 1 and preparation method thereof, it is characterised in that secondary dry described in step (1)
Lactobacillus paracasei CICC6270 and Lactobacillus paracasei CICC6271 uses based seed media (to add 50% asparagus juice and 2% Portugal
Grape sugar), 37 DEG C of quiescent culture, activate 16-30 h.
Germinatus Phragmitis fermented beverage the most according to claim 1 and preparation method thereof, it is characterised in that secondary described in step (1)
Lactobacillus casei CICC6270 and Lactobacillus paracasei CICC6271 volume proportion are 3:1～3:5, control fermentation temperature be 15～
40 DEG C, fermentation time is 12～96 h.
Germinatus Phragmitis fermented beverage the most according to claim 1 and preparation method thereof, it is characterised in that described in step (2)
γ-aminobutyric acid, Semen Ziziphi Spinosae extract, Radix Ginseng extract, Fructus Schisandrae Chinensis extrat and the quality volume basis of Germinatus Phragmitis fermentation natural juice
Than respectively be 0.05～0.2 %, 0.02～0.4 %, 0.015～0.3%, 0.03～0.25%.
6. in Claims 1 to 5, Germinatus Phragmitis fermented beverage described in any one in preparation hypnosis or improves the medicine of sleep, food
Or the purposes in health product.
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| CN111642747A (en) * | 2019-07-23 | 2020-09-11 | 河北一然生物科技有限公司 | Application of narqu 4580 probiotics and preparation in food for improving sleep quality |
| CN112089055A (en) * | 2020-09-22 | 2020-12-18 | 上海泓商生物科技有限公司 | Pressure-reducing sleep-relaxing probiotic composition |
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| CN116035069A (en) * | 2020-11-26 | 2023-05-02 | 内蒙古伊利实业集团股份有限公司 | Lactobacillus paracasei K56 for regulating sleep and application thereof |
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Address after: No. 32, Beijing Xiaoyun Road, Chaoyang District, Beijing Applicant after: CHINA NATIONAL RESEARCH INSTITUTE OF FOOD & FERMENTATION INDUSTRIES Co.,Ltd. Applicant after: BEI JING XNK RESEARCH CENTRE OF ASPARAGUS Address before: No. 32, Beijing Xiaoyun Road, Chaoyang District, Beijing Applicant before: CHINA NATIONAL Research Institute OF FOOD AND FERMENTATION INDUSTRIES Applicant before: BEI JING XNK RESEARCH CENTRE OF ASPARAGUS |
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