CN106070059A - A kind of method measuring Rana nigromaculata acoustics ability to communicate - Google Patents
A kind of method measuring Rana nigromaculata acoustics ability to communicate Download PDFInfo
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- CN106070059A CN106070059A CN201610591814.6A CN201610591814A CN106070059A CN 106070059 A CN106070059 A CN 106070059A CN 201610591814 A CN201610591814 A CN 201610591814A CN 106070059 A CN106070059 A CN 106070059A
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- 241000270959 Pelophylax nigromaculatus Species 0.000 title claims abstract description 160
- 238000000034 method Methods 0.000 title claims abstract description 42
- 239000011521 glass Substances 0.000 claims abstract description 29
- 230000008569 process Effects 0.000 claims abstract description 22
- 241001465754 Metazoa Species 0.000 claims abstract description 13
- 230000013011 mating Effects 0.000 claims abstract description 4
- 238000002474 experimental method Methods 0.000 claims description 25
- 230000005115 phonotaxis Effects 0.000 claims description 23
- 239000003814 drug Substances 0.000 claims description 12
- 229940079593 drug Drugs 0.000 claims description 11
- 238000002347 injection Methods 0.000 claims description 11
- 239000007924 injection Substances 0.000 claims description 11
- 102000011022 Chorionic Gonadotropin Human genes 0.000 claims description 10
- 108010062540 Chorionic Gonadotropin Proteins 0.000 claims description 10
- 229940084986 human chorionic gonadotropin Drugs 0.000 claims description 10
- 238000012360 testing method Methods 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 238000001228 spectrum Methods 0.000 claims description 5
- 101100117236 Drosophila melanogaster speck gene Proteins 0.000 claims description 4
- 230000033001 locomotion Effects 0.000 claims description 4
- 230000004044 response Effects 0.000 claims description 4
- 238000004458 analytical method Methods 0.000 claims description 3
- 230000027326 copulation Effects 0.000 claims description 3
- 238000002203 pretreatment Methods 0.000 claims description 3
- 210000004556 brain Anatomy 0.000 claims 1
- 230000005611 electricity Effects 0.000 claims 1
- 238000004088 simulation Methods 0.000 claims 1
- 239000002689 soil Substances 0.000 claims 1
- 230000036541 health Effects 0.000 abstract description 4
- 230000007613 environmental effect Effects 0.000 abstract description 3
- 239000008239 natural water Substances 0.000 abstract 1
- 238000004891 communication Methods 0.000 description 12
- 238000003556 assay Methods 0.000 description 3
- 230000008901 benefit Effects 0.000 description 2
- 210000004907 gland Anatomy 0.000 description 2
- 241000132931 Batrachia Species 0.000 description 1
- 102000006771 Gonadotropins Human genes 0.000 description 1
- 108010086677 Gonadotropins Proteins 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000013480 data collection Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000002622 gonadotropin Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
- Animal Behavior & Ethology (AREA)
- Marine Sciences & Fisheries (AREA)
Abstract
The invention discloses a kind of method measuring Rana nigromaculata acoustics ability to communicate, comprise the following steps: from natural water, gather Rana nigromaculata；Process group and matched group are set, Rana nigromaculata is carried out nature pairing process；Glass aquarium endoadaptation 5 15min that all of Rana nigromaculata is placed in dark room；In advance the normal female of mating period and the tweeting sound of male Rana nigromaculata are recorded；Play normal Rana nigromaculata tweeting sound, observe the reaction of the Rana nigromaculata of different disposal group, and corresponding tweeting sound and data are carried out record；Acquisition pipes parameter, analyzes experimental data.The present invention is by further appreciating that the relation of song and animal behavior and efficiently the health of this animal of reflection and the environmental aspect lived provide important indicative significance.
Description
Technical field
The present invention relates to assay method technical field, particularly relate to a kind of method measuring Rana nigromaculata acoustics ability to communicate.
Background technology
Acoustics communication, is that the sound sent makes other individualities or colony receive and produce the communication modes of behavior reaction.
Animal can send the ring of different frequency, intensity and waveform under different behavior mechanisms according to kind and physiological status difference thereof
Sound, song is neglected and is now suddenly stopped there is precise time, to reach the purpose of accurate communication.
The song of animal is the language of mutually communication between individuality in population, be with cluster, take food, field sets up and defendance,
The communication behavior that breeding etc. are movable and relevant to harmful animal reaction.By the research to animal acoustics communication, ring can be further appreciated that
Sound and the relation of behavior, the health efficiently reflecting this animal and the environmental aspect lived, have important indicative significance.
At present, a kind of method measuring batrachia acoustics communication is still lacked.Therefore, it is necessary to specific aim proposes one measures the frog
The method of class acoustics communication reflects its health or habitat situation.
Summary of the invention
The technical problem to be solved is to provide a kind of assay method reacting animal acoustics communication.
For solving above-mentioned technical problem, the present invention provides the assay method of a kind of Rana nigromaculata acoustics communication, including following step
Rapid:
(1): gather Rana nigromaculata
Gathering from nature water body and be in the Rana nigromaculata of marriage phase as experimental subject, each 5-10 of male and female is only；
(2): natural pairing processes
If arranging dry-cure group and matched group, often group includes 1 female and male Rana nigromaculata；By process group and matched group
Rana nigromaculata is placed in Control Room environment and adapts to after 3-5d, and human chorionic gonadotropin's gland that the Rana nigromaculata of process group carries out variable concentrations swashs
Element drug treating, female Rana nigromaculata lumbar injection 500-1000 unit human chorionic gonadotropin, male Rana nigromaculata is injected
300-500 unit human chorionic gonadotropin, matched group is then with injection water alternatives to medication；
(3): experimental data and the acquisition of the parameter that pipes
The mode of piping of marriage phase female Rana nigromaculata includes that acceptance pipes and refusal pipes, the mode that pipes of male Rana nigromaculata
Pipe including advertisement and response pipes；
The acceptance of the normal female Rana nigromaculata that broadcasting records in advance pipes and male advertisement pipes, and carries out phonotaxis real
Test, observe the reaction of the male and female Rana nigromaculata of different disposal group, obtain corresponding data and the parameter that pipes.
Preferably, the acquisition of described experimental data and the parameter that pipes comprises the following specific steps that:
(1): experiment pre-treatment
Before phonotaxis experiment starts, the glass aquarium endoadaptation 10-that all of Rana nigromaculata is placed in dark room
15min；
(2): the acquisition of normal Rana nigromaculata tweeting sound
Gather the normal female of mating period and male Rana nigromaculata in advance, process without drug injection, be respectively put into one
In the glass jar of experimental provision both sides, the acceptance of female Rana nigromaculata is piped and the advertisement of male Rana nigromaculata pipes and records respectively,
And whole process video recording, until copulation success；
(3): experiment Rana nigromaculata tweeting sound and the acquisition of data
Rana nigromaculata in step (1) is placed individually in experimental provision and tests in the glass jar of side, distance means
Speaker 40-60cm in opposite side glass jar；The acceptance playing the female Rana nigromaculata recorded in advance with speaker continuously pipes
Or the advertisement of male Rana nigromaculata pipes, observe male Rana nigromaculata and accept female Rana nigromaculata to pipe or female Rana nigromaculata is to male black
The reaction that speckle frog advertisement pipes, records its time starting movement；When the cry of female Rana nigromaculata or male Rana nigromaculata induces hero
After property Rana nigromaculata or female Rana nigromaculata start to pipe, pipe from it and proceed by observation, record it in 5-10min by sound
The time persistently piped after sound induction, every frog repeats at least 3 times；Experimentation carries out omnidistance recording and video recording, obtains institute
Have the tweeting sound that experiment Rana nigromaculata is individual, record the quantity of active Rana nigromaculata, distance that Rana nigromaculata is moved, away from close
Time of speaker, pipe and time of moving first first；
(4): the acquisition of the parameter that pipes and the analysis of experimental data
The sound spectrum that pipes that each Rana nigromaculata obtained is individual is detected, identifies sound type, observe and record each
The parameter of cry, including oscillogram, spectrogram, amplitude, formant, duration, dominant frequency, fundamental frequency and cry syllable number；To Rana nigromaculata
The data obtained in phonotaxis experiment are analyzed.
Preferably, in described phonotaxis experimentation, in order to avoid potential subjectivity, dense by each process group Rana nigromaculata
Scale designation is blocked by paper slip, makes each group of drug level unknown.
Preferably, in described phonotaxis experimentation, for the propagation that beneficially Rana nigromaculata pipes, experimental provision is designed as
One both sides are equipped with a glass jar respectively and centre connects aisled device.
Preferably, in phonotaxis experimentation, for simulating natural environment, Rana nigromaculata is positioned over the glass indicating distance
In cylinder, glass jar limit is marked with scale, is covered with earth bottom glass jar.
Preferably, before the experiment of described phonotaxis, in order to avoid the opposite sex Rana nigromaculata tweeting sound impact on experimental subject, make hero
Property or female Rana nigromaculata avoid exposure in the tweeting sound of female or male Rana nigromaculata.
Preferably, in described phonotaxis experimentation, in order to get rid of the noise impact on experiment, filtration in advance records
The background noise of normal Rana nigromaculata.
Preferably, in above-mentioned all phonotaxis experimentations, by automatic wild animal cry recorder to female and
Piping of male Rana nigromaculata is recorded, and is played out recording by speaker, by camera to female and male black speck
The behavior of the frog is recorded a video, and connects photographic head by computer and video recording is carried out computer monitor.
Preferably, in above-mentioned all phonotaxis experimentations, camera is positioned over the side of glass jar, is set to night red
Outer logging mode, is placed in infrared camera above glass jar, and watch-dog is placed directly in the top of glass jar side.
Preferably, above-mentioned all of phonotaxis is tested, and carries out in a dark room of 20:00-06:00 in evening.
It is an advantage of the current invention that: the present invention is by providing a kind of method measuring Rana nigromaculata acoustics ability to communicate, for entering
One step understands the relation of song and animal behavior, the health efficiently reflecting this animal and the environmental aspect lived, it is provided that
Important indicative significance.
Accompanying drawing explanation
Fig. 1 is the flow chart of a kind of method measuring Rana nigromaculata acoustics ability to communicate that the embodiment of the present invention provides；
Fig. 2 is the oscillogram that the male Rana nigromaculata advertisement that the embodiment of the present invention provides pipes；
Fig. 3 is the spectrogram that the male Rana nigromaculata advertisement that the embodiment of the present invention provides pipes；
Fig. 4 is the sound pressure level figure that the male Rana nigromaculata advertisement that the embodiment of the present invention provides pipes；
Fig. 5 is the formant figure that the male Rana nigromaculata advertisement that the embodiment of the present invention provides pipes；
Fig. 6 is the oscillogram that the female Rana nigromaculata that the embodiment of the present invention provides accepts to pipe；
Fig. 7 is the spectrogram that the female Rana nigromaculata that the embodiment of the present invention provides accepts to pipe；
Fig. 8 is the sound pressure level figure that the female Rana nigromaculata that the embodiment of the present invention provides accepts to pipe；
Fig. 9 is the formant figure that the female Rana nigromaculata that the embodiment of the present invention provides accepts to pipe.
Detailed description of the invention
Elaborating embodiments of the invention below, the present embodiment is carried out under premised on technical solution of the present invention
Implement, give detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following enforcement
Example.
A kind of method measuring Rana nigromaculata acoustics ability to communicate of the present embodiment, as it is shown in figure 1, comprise the steps:
(1) gather Rana nigromaculata
Gather from nature water body and be in the Rana nigromaculata of marriage phase as experimental subject, each 5 of male and female；
(2) natural pairing processes
Arranging 4 process groups and 1 matched group, often group includes 1 female and male Rana nigromaculata；
The Rana nigromaculata of process group and matched group is placed in after Control Room environment adapts to 3d and carries out different drug treating: to place
The Rana nigromaculata of reason group carries out human chorionic gonadotropin (the Human chorionic of lumbar injection not commensurate
Gonadotropin, hCG) drug treating, female Rana nigromaculata chooses 500,800,900 and 1,000 4 unit gradients, male black
The speckle frog chooses 300,350,400 and 500 4 unit gradients the most accordingly；Matched group is with water alternatives to medication (human chorionic gonadotropin's gland
Piping and copulation of hormone beneficially Rana nigromaculata, female lumbar injection 500-1000 unit human chorionic gonadotropin, male
Injection 300-500 unit human chorionic gonadotropin can be induced and be piped)；
(3) experimental data and the acquisition of the parameter that pipes
Marriage phase female Rana nigromaculata mainly sends acceptance and pipes and refusal pipes, and male Rana nigromaculata mainly sends advertisement and pipes
Piping with response, these four pipes and has different sound spectrums；
The acceptance of the normal female Rana nigromaculata that broadcasting records in advance pipes and male advertisement pipes, and carries out phonotaxis real
Test, observe the reaction of male and female Rana nigromaculata of different disposal group, and obtain corresponding data and the parameter that pipes, specifically include with
Lower step:
1. pre-treatment is tested
Carry out in one dark room of all of phonotaxis experiment 20:00-06:00 the most at night, and in order to avoid
Potential subjectivity, experiment is all carried out (with dense each process group echo of paper slip in the case of process group drug level the unknown
Degree shelters from)；
Before phonotaxis experiment, in order to avoid the interference of speaker in later experiments, all of Rana nigromaculata 10min first to go to fit
Answer the glass aquarium in dark house, if male Rana nigromaculata is away from speaker, then experiment starts, if male black speck
The frog does not has stop motion, and it will be removed, and waits until that this frog is tested after terminating by other individual experiments the most again；
2. the acquisition of normal Rana nigromaculata tweeting sound
Gather the normal female of mating period and male Rana nigromaculata in advance, process without drug injection, be respectively put into experiment
In the glass jar of device both sides；This experimental provision is that both sides are equipped with a glass jar respectively and centre is connected by a path
The device connect；After Rana nigromaculata is successfully put into experimental provision, by automatic wild animal cry recorder to female Rana nigromaculata
Acceptance pipe and the advertisement of male Rana nigromaculata pipes and records respectively, and filter background noise, recorded a video by camera whole process, directly
Best friend is made into merit；
3. acquisition and the data collection of Rana nigromaculata tweeting sound are tested
Female Rana nigromaculata close to lay eggs but be not introduced into embrace to when, can discharge acceptance pipe attract male black
Speckle frog entering active phase；And male Rana nigromaculata is in order to cause the concern of female Rana nigromaculata, will send advertisement pipe displaying from
The advantage of body；
By step (1) in Rana nigromaculata be placed individually in the glass jar indicating distance of experimental provision side, on glass jar limit
Indicate scale, and bottom is paved with earth, simulating natural environment；Before phonotaxis experiment, male or female Rana nigromaculata is also not exposed to
In the female or tweeting sound of male Rana nigromaculata；Then, with being placed in opposite side glass jar and the speaker of distance Rana nigromaculata 50cm
The acceptance of the female Rana nigromaculata that continuous broadcasting is recorded in advance pipes or the advertisement of male Rana nigromaculata pipes, and (it is black that use is recorded in advance
Speckle croak is conducive to the control of the parameter of cry, gets rid of the interference of other crys, because other cry is likely to be black speck
The frog sends under different reproductive status, or has other special communication meaning), observe male Rana nigromaculata to female black
The speckle frog accepts the reaction piped or male Rana nigromaculata advertisement is piped by female Rana nigromaculata, records its time starting movement；When female
After property Rana nigromaculata or the cry induced male Rana nigromaculata of male Rana nigromaculata or female Rana nigromaculata start to pipe, from the beginning of it pipes
Observing, record the time persistently piped after it is induced by sound in 5min, every frog is repeated 3 times；
The phonotaxis behavior of Rana nigromaculata is mainly recorded a video by camera, record Rana nigromaculata move away from discrete time:
Camera is placed on the side of water vat, is set to pattern in evening (infrared record pattern), is placed in by infrared camera on experiment water vat
Side, watch-dog is placed directly in the top of experiment water vat side, it is ensured that be able to observe that whole Experimental Area；By photographic head and computer
Connect, observe video recording by computer；
Carrying out omnidistance recording and video recording in experimentation, obtain the tweeting sound that all experiment Rana nigromaculatas are individual, record has
Distance that the activity quantity of Rana nigromaculata, Rana nigromaculata are moved, away from near speaker time, pipe and move first first
Time；
The acquisition of the parameter that 4. pipes and the analysis of experimental data
The sound spectrum that pipes using the Praat software each Rana nigromaculata to obtaining individual detects, and identifies sound type: wide
Announcement pipes, response pipes, accept to pipe and refusal pipes, and observes and record the parameter of each cry, including oscillogram, frequency spectrum
Figure, amplitude, formant, duration, dominant frequency, fundamental frequency and cry syllable number；The data obtained in testing Rana nigromaculata phonotaxis are carried out
Analyze.
By the method for said determination Rana nigromaculata acoustics ability to communicate, determine the acoustics communication feature of Rana nigromaculata.When broadcasting
Putting the acceptance of female Rana nigromaculata and pipe when attracting the male processed, male Rana nigromaculata have issued advertisement and pipes,
Respective waveforms figure such as Fig. 2, spectrogram such as Fig. 3, sound pressure level figure such as Fig. 4, formant figure such as Fig. 5, and in experimentation
Show and female Rana nigromaculata is piped the most close；When the advertisement playing male Rana nigromaculata pipes to the female individuals processed
When attracting, female Rana nigromaculata have issued acceptance and pipes, respective waveforms figure such as Fig. 6, spectrogram such as Fig. 7, sound pressure level
Figure such as Fig. 8, formant figure such as Fig. 9, and show in experimentation male Rana nigromaculata is piped the most close.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all essences in the present invention
Any amendment, equivalent and the improvement etc. made within god and principle, should be included within the scope of the present invention.
Claims (10)
1. the method measuring Rana nigromaculata acoustics ability to communicate, it is characterised in that comprise the steps:
(1): gather Rana nigromaculata
Gathering from nature water body and be in the Rana nigromaculata of marriage phase as experimental subject, each 5-10 of male and female is only；
(2): natural pairing processes
If arranging dry-cure group and matched group, often group includes 1 female and male Rana nigromaculata；By process group and the black speck of matched group
The frog is placed in Control Room environment and adapts to after 3-5d, and the Rana nigromaculata of process group carries out the human chorionic gonadotropin medicine of variable concentrations
Thing processes, female Rana nigromaculata lumbar injection 500-1000 unit human chorionic gonadotropin, male Rana nigromaculata injection 300-500
Unit human chorionic gonadotropin, matched group is then with injection water alternatives to medication；
(3): experimental data and the acquisition of the parameter that pipes
The mode of piping of marriage phase female Rana nigromaculata includes that acceptance pipes and refusal pipes, and the mode of piping of male Rana nigromaculata includes
Advertisement pipes and response pipes；
The acceptance of the normal female Rana nigromaculata that broadcasting records in advance pipes and male advertisement pipes, and carries out phonotaxis experiment,
Observe the reaction of the male and female Rana nigromaculata of different disposal group, obtain corresponding data and the parameter that pipes.
A kind of method measuring Rana nigromaculata acoustics ability to communicate the most according to claim 1, it is characterised in that experimental data
And the acquisition of the parameter that pipes comprises the following specific steps that:
(1): experiment pre-treatment
Before phonotaxis experiment starts, the glass aquarium endoadaptation 10-15min that all of Rana nigromaculata is placed in dark room；
(2): the acquisition of normal Rana nigromaculata tweeting sound
Gather the normal female of mating period and male Rana nigromaculata in advance, process without drug injection, be respectively put into an experiment
In the glass jar of device both sides, the acceptance of female Rana nigromaculata is piped and the advertisement of male Rana nigromaculata pipes and records respectively, and entirely
Journey is recorded a video, until copulation success；
(3): experiment Rana nigromaculata tweeting sound and the acquisition of data
Rana nigromaculata in step (1) is placed individually in experimental provision and tests in the glass jar of side, distance means another
Speaker 40-60cm in the glass jar of side；The acceptance playing the female Rana nigromaculata recorded in advance with speaker continuously pipes or male
Property Rana nigromaculata advertisement pipe, observe male Rana nigromaculata and accept to pipe or female Rana nigromaculata is to male Rana nigromaculata to female Rana nigromaculata
The reaction that advertisement pipes, records its time starting movement；When the cry induced male of female Rana nigromaculata or male Rana nigromaculata is black
After the speckle frog or female Rana nigromaculata start to pipe, pipe from it and proceed by observation, record it and lured by sound in 5-10min
The time persistently piped after leading, every frog repeats at least 3 times；Experimentation carries out omnidistance recording and video recording, obtains all realities
Test the tweeting sound that Rana nigromaculata is individual, record the quantity of active Rana nigromaculata, distance that Rana nigromaculata is moved, away from near raising one's voice
Time of device, pipe and time of moving first first；
(4): the acquisition of the parameter that pipes and the analysis of experimental data
The sound spectrum that pipes that each Rana nigromaculata obtained is individual is detected, identifies sound type, observe and record each cry
Parameter, including oscillogram, spectrogram, amplitude, formant, duration, dominant frequency, fundamental frequency and cry syllable number；Become sound to Rana nigromaculata
Property experiment in obtain data be analyzed.
A kind of method measuring Rana nigromaculata acoustics ability to communicate the most according to claim 2, it is characterised in that phonotaxis is real
During testing, the concentration markers of each process group Rana nigromaculata is blocked by paper slip.
A kind of method measuring Rana nigromaculata acoustics ability to communicate the most according to claim 2, it is characterised in that experimental provision
It is that both sides are equipped with a glass jar and the middle device connected by a path respectively.
A kind of method measuring Rana nigromaculata acoustics ability to communicate the most according to claim 2, it is characterised in that phonotaxis is real
During testing, Rana nigromaculata is positioned in the glass jar indicating distance, and glass jar limit is marked with scale, and the bottom of glass jar spreads mud
Soil, the environment of simulation nature.
A kind of method measuring Rana nigromaculata acoustics ability to communicate the most according to claim 2, it is characterised in that phonotaxis is real
Before testing, male or female Rana nigromaculata is also not exposed to the tweeting sound of female or male Rana nigromaculata.
A kind of method measuring Rana nigromaculata acoustics ability to communicate the most according to claim 2, it is characterised in that filter in advance
The background noise of the normal Rana nigromaculata recorded.
A kind of method measuring Rana nigromaculata acoustics ability to communicate the most according to claim 2, it is characterised in that by automatically
Wild animal cry recorder female and male Rana nigromaculata piped record, by speaker, recording is broadcast
Put, by camera, the behavior of female and male Rana nigromaculata is recorded a video, connect photographic head by computer and video recording is carried out electricity
Brain monitors.
A kind of method measuring Rana nigromaculata acoustics ability to communicate the most according to claim 8, it is characterised in that camera is put
Being placed in the side of glass jar, be set to infrared record pattern at night, be placed in by infrared camera above glass jar, watch-dog is direct
It is placed on the top of glass jar side.
10. according to a kind of method measuring Rana nigromaculata acoustics ability to communicate described in claim 1-9, it is characterised in that all
Phonotaxis experiment 20:00-06:00 the most at night a dark room in carry out.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1709049A (en) * | 2005-07-08 | 2005-12-21 | 刘国民 | Frog artificial breeding and rearing method |
| TW201117190A (en) * | 2009-11-12 | 2011-05-16 | Nat Cheng Kong University | Sound recognition for frog vocalizations |
| US20120230543A1 (en) * | 2000-11-06 | 2012-09-13 | Nant Holdings Ip Llc | Object Information Derived from Object Images |
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| CN1709049A (en) * | 2005-07-08 | 2005-12-21 | 刘国民 | Frog artificial breeding and rearing method |
| TW201117190A (en) * | 2009-11-12 | 2011-05-16 | Nat Cheng Kong University | Sound recognition for frog vocalizations |
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| 王玉胜: "雄性非洲爪蟾鸣叫行为的调控机制", 《中国优秀硕士学位论文集 基础科学辑》 * |
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