CN106066404A - A kind of enzyme mark microwell plate loading instruction control system and control method - Google Patents
A kind of enzyme mark microwell plate loading instruction control system and control method Download PDFInfo
- Publication number
- CN106066404A CN106066404A CN201610514637.1A CN201610514637A CN106066404A CN 106066404 A CN106066404 A CN 106066404A CN 201610514637 A CN201610514637 A CN 201610514637A CN 106066404 A CN106066404 A CN 106066404A
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- China
- Prior art keywords
- circuit
- led
- microwell plate
- enzyme mark
- controller
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
-
- H—ELECTRICITY
- H05—ELECTRIC TECHNIQUES NOT OTHERWISE PROVIDED FOR
- H05B—ELECTRIC HEATING; ELECTRIC LIGHT SOURCES NOT OTHERWISE PROVIDED FOR; CIRCUIT ARRANGEMENTS FOR ELECTRIC LIGHT SOURCES, IN GENERAL
- H05B45/00—Circuit arrangements for operating light-emitting diodes [LED]
- H05B45/20—Controlling the colour of the light
Abstract
The invention discloses a kind of enzyme mark microwell plate loading instruction control system and control method, relate to enzyme mark microwell plate and carry out manual loading technical field, this system includes: LED indicates dot matrix circuit, described LED instruction dot matrix circuit includes LED matrix arranged in arrays, the LED matrix of described LED instruction dot matrix circuit is corresponding with the microporous matrix of enzyme mark microwell plate, the corresponding input of described LED of often going, the corresponding outfan of LED described in each column；Controller, the I/O interface of controller is connected with the input of LED instruction dot matrix circuit；The logic circuit being connected with controller signals, logic circuit is connected with the outfan of LED instruction dot matrix circuit；The power circuit being connected with controller and logic circuit；Be connected man-machine interaction touch screen with controller；The button being connected with controller triggers circuit, and button triggers circuit and has forwarding button, back, pause button and SR.The present invention has easy to operate, loading efficiency height and the advantage of low cost.
Description
Technical field
During the present invention relates to elisa, enzyme mark microwell plate carries out manual loading technical field, specifically relates to
And one enzyme mark microwell plate loading indicates control system and control method.
Background technology
Enzyme-linked immunosorbent assay (Enzyme Linked ImmunoSorbent Assay), is called for short ELISA, is to adopt
With the specific reaction of antigen Yu antibody, determinand is linked with enzyme, then produce color reaction by enzyme-to-substrate, to being examined
Material carries out qualitative or a kind of detection method of quantitative analysis.ELISA is as a new technique of immunologic diagnosis, with its spirit
Quick, special, simple, quick, stable feature is widely used in the fields such as scientific research, inspection and quarantine, clinical assays.
Enzyme-linked immunosorbent assay is widely used, its basic process include enzyme mark microwell plate loading, sample incubation, first
Secondary wash plate, enzyme conjugates is hatched, second time washes plate, substrate colour developing, microplate reader read the several process of plate, for different detection samples
Product, its processing procedure may be different, and this process is mainly completed by reviewer's manual operations at present.
Enzyme mark microwell plate is 96 holes and 384 holes, and reviewer needs to use single channel pipettor or Multi-channel liquid transfer device
Enzyme-linked Immunosorbent Assay process according to different things to be checked carries out loading.Owing to loading step is numerous and diverse, plate hole number is many, sample loading mode
Changing various, band is examined specimen one_to_one corresponding adding microwell plate plate hole is a loaded down with trivial details job, and long-time continuous surface is to 96 holes
Plate or 384 orifice plate operations can cause operator's spirit high-pressure, loading mistake easily occur.It addition, during loading by
In certain reason need suspend loading time, when one section after again loading time, it may appear that forget early stage ignition mode and loading
Step causes obtaining the situation of loading again.Two kinds of error situations all can cause the waste of ELISA reagent and sample, and repeatability
Work can reduce work efficiency.
Summary of the invention
For defect present in prior art, it is an object of the invention to provide a kind of enzyme mark microwell plate loading instruction control
System processed and control method, it is possible to the manual loading position of prompting and ignition mode, effectively solve the situation that manual loading is made mistakes, tool
There are easy to operate, loading efficiency height and the advantage of low cost.
For reaching object above, the present invention adopts the technical scheme that:
A kind of enzyme mark microwell plate loading instruction control system, including:
LED indicates dot matrix circuit, described LED instruction dot matrix circuit to include LED matrix arranged in arrays, described LED
The LED matrix of instruction dot matrix circuit is corresponding with the microporous matrix of enzyme mark microwell plate, the corresponding input of described LED of often going,
The corresponding outfan of LED described in each column；
Controller, the I/O interface of described controller is connected with the described input of described LED instruction dot matrix circuit, described control
Device processed is for controlling the ignition mode of the LED in described LED instruction dot matrix circuit；
The logic circuit being connected with described controller signals, described logic circuit indicates the institute of dot matrix circuit with described LED
State outfan to be connected；
The power circuit being connected with described controller and described logic circuit, for described controller and described logic electricity
Road powers；
Be connected man-machine interaction touch screen with described controller, for providing the user editor and controlling the interface of operation；
The button being connected with described controller triggers circuit, provides for described controller and triggers signal.
On the basis of technique scheme, this system also includes condition indication circuit, described condition indication circuit and institute
State controller to be connected, and described condition indication circuit includes or left tutorial light and lower or right tutorial light.
On the basis of technique scheme, described logic circuit includes some multiway analog switches and a bus switch,
Described multiway analog switch and described bus switch are connected with described controller signals respectively.
On the basis of technique scheme, described ignition mode includes ignition mode, row ignition mode and row point one by one
Bright pattern.
On the basis of technique scheme, described ignition mode one by one includes with row pattern lightening LED lamp one by one and row
Pattern lightening LED lamp one by one.
On the basis of technique scheme, the LED matrix of described LED instruction dot matrix circuit is 8*12 matrix or 16*
24 matrixes.
On the basis of technique scheme, this system also includes foot switch control circuit, and described foot switch controls
Circuit is connected with described controller.
On the basis of technique scheme, this system also includes peripheral circuit, described peripheral circuit and described controller
Being connected, described peripheral circuit includes clock circuit, reset circuit and download program circuit.
A kind of enzyme mark microwell plate loading indication control method, specifically comprises the following steps that
S1, user is by the ignition mode in man-machine interaction touch screen selection control；
S2, user is according to ignition mode to the plate hole loading of enzyme mark microwell plate, and the every loading of user once, light by controller
The LED of current loading position in LED instruction dot matrix circuit；
S3, user is according to ignition mode, and circulation step S2, successively to the plate hole loading of enzyme mark microwell plate, until completing enzyme
Mark microwell plate loading.
On the basis of technique scheme, also include S4, after enzyme mark microwell plate completion of the sample, operate SR, institute
LED is had to be turned off.
Compared with prior art, it is an advantage of the current invention that:
(1) a kind of enzyme mark microwell plate loading instruction control system of the present invention includes LED dot matrix circuit, controller and logic
Circuit, LED dot matrix circuit include LED matrix arranged in arrays, LED dot matrix circuit have several inputs and several
Outfan, when needs a line is lighted, the input of this row and the outfan of all row are joined by controller control logic circuit
Logical, when needs string is lighted, controller control logic circuit, by the outfan UNICOM of all inputs He these row, works as needs
When a certain LED in matrix is lighted, by row input corresponding for this LED and row outfans UNICOM, pass through controller
Control that there is multiple ignition mode, simple in construction, easy to operate, use flexibly, loading speed can be effectively improved.
(2) a kind of enzyme mark microwell plate loading instruction control system of the present invention includes that foot switch control circuit and state refer to
Showing circuit, can be lighted the LED of loading position by foot switch control circuit, easy to operate, humanization designing, state refers to
Show that circuit has upper or left display lamp and lower or right indicating lamp, after suspending a period of time, when carrying out loading operation, can basis
Ignition mode on touch screen and the mark of display lamp, the again direction of loading and position, it is therefore prevented that the situation that loading is made mistakes, subtract
Few observing time, improve loading speed.
Accompanying drawing explanation
Fig. 1 is the structured flowchart of control system in embodiments of the invention；
Fig. 2 is the schematic circuit diagram of LED dot matrix circuit in the embodiment of the present invention；
Fig. 3 is the circuit diagram of multichannel analog on-off circuit in the embodiment of the present invention；
Fig. 4 is the circuit diagram of multichannel analog on-off circuit in the embodiment of the present invention；
Fig. 5 is the circuit diagram of multichannel analog on-off circuit in the embodiment of the present invention；
Fig. 6 is the circuit diagram of bus switch circuit in the embodiment of the present invention.
Detailed description of the invention
Below in conjunction with drawings and Examples, the present invention is described in further detail.
Shown in Figure 1, the embodiment of the present invention provides a kind of enzyme mark microwell plate loading instruction control system, including:
Shown in Figure 2, LED indicates dot matrix circuit, LED instruction dot matrix circuit to include the LED square in M*N matrix arrangement
Battle array, the LED matrix of LED instruction dot matrix circuit is corresponding with the microporous matrix of enzyme mark microwell plate, and in the present embodiment, LED indicates point
The LED matrix of battle array circuit is that 16*24, LED instruction dot matrix circuit has 16 input AMWith 24 output terminals A-AN, and often go
The corresponding input of LED, the corresponding outfan of each column LED.
Controller, the I/O interface of controller is connected with the input of 16 row of LED instruction dot matrix circuit, and controller is used for controlling
The ignition mode of the LED in LED processed instruction dot matrix circuit；
Seeing shown in Fig. 3, Fig. 4 and Fig. 5, wherein Fig. 3, Fig. 4 and Fig. 5 are multi-channel analog switch circuit figure, logic circuit
Including three multiway analog switches and a piece of 24 BITBUS network switches, and the model of multiway analog switch is CD4051B, three multichannels
The chip of analog switch enables signal INH and channel selecting signal ABC and is connected with the I/O port of controller, and controller controls multichannel mould
Intend enable and the channel switching of switch, realized the on-off control of 24 autonomous channels by three analog switches, indicate with LED
24 row correspondences of dot matrix circuit, i.e. by the controller control to three multi-channel analog circuit, it is achieved individually controls LED and indicates point
The level of 24 row of battle array circuit.
Shown in Figure 6, for bus switch circuit figure, the model of a piece of 24 BITBUS network switches is SN74CBT16212A, always
Control signal end S0, S1, S2 of wiretap are connected with the I/O port of controller, and controller controls S0, S1, S2 can realize each passage
While switch, with LED instruction dot matrix circuit 24 row corresponding, can realize LED dot matrix 24 row be all high level or while
For low level.
The power circuit being connected with controller and logic circuit, for controller and power logic circuitry.
Be connected man-machine interaction touch screen with controller, for providing the user editor and controlling the interface of operation.
The button being connected with controller triggers circuit, and button triggers circuit and has forwarding button, back, pause button
And SR, trigger signal for providing for controller.
The condition indication circuit being connected with controller, condition indication circuit includes or left tutorial light and lower or right behaviour
Make display lamp.
The foot switch control circuit being connected with controller, foot switch control circuit is connected with controller.
The peripheral circuit being connected with controller, peripheral circuit includes clock circuit, reset circuit and download program circuit.
Ignition mode includes ignition mode, row ignition mode and row ignition mode one by one, and ignition mode includes the most one by one
Row pattern lightening LED lamp one by one and row mode lightening LED lamp one by one.
Row mode lightening LED lamp one by one refers to often to go light the most one by one the LED of this row, when the LED of a line
After lighting, light the LED of every a line the most successively；Row pattern lightening LED lamp one by one refers to that each column is the most one by one
Light the LED of these row, after the LED of string point is lighted, light the LED of every string the most successively；Row mode is
Refer to light the LED of a line every time, light often row LED the most successively；Row pattern refers to light the LED of string every time
Lamp, lights each column LED the most successively.
A kind of enzyme mark microwell plate loading indication control method, specifically comprises the following steps that
S1, user is by the ignition mode in man-machine interaction touch screen selection control, and wherein ignition mode includes row mould
Formula lightening LED lamp one by one, row pattern lightening LED lamp one by one, row mode light a line LED line by line and row pattern lights one by column
Row LED；
S2, user is according to ignition mode to the plate hole loading of enzyme mark microwell plate, and the every loading of user once, light by controller
The LED of current loading position in LED instruction dot matrix circuit；
User can control single led lamp, single LED or single-row LED by forwarding button and back and move,
In loading operating process, by the turned-on position of LED during the halted state of pause button location；
S3, user is according to ignition mode, and circulation step S2, successively to the plate hole loading of enzyme mark microwell plate, until completing enzyme
Mark microwell plate loading；
S4, after enzyme mark microwell plate completion of the sample, by SR, all LED are turned off.
The present invention is not limited to above-mentioned embodiment, for those skilled in the art, without departing from
On the premise of the principle of the invention, it is also possible to make some improvements and modifications, these improvements and modifications are also considered as the protection of the present invention
Within the scope of.The content not being described in detail in this specification belongs to prior art known to professional and technical personnel in the field.
Claims (10)
1. an enzyme mark microwell plate loading instruction control system, it is characterised in that including:
LED indicates dot matrix circuit, described LED instruction dot matrix circuit to include LED matrix arranged in arrays, and described LED indicates
The LED matrix of dot matrix circuit is corresponding with the microporous matrix of enzyme mark microwell plate, the corresponding input of described LED of often going, each column
The corresponding outfan of described LED；
Controller, the I/O interface of described controller is connected with the described input of described LED instruction dot matrix circuit, described controller
For controlling the ignition mode of the LED in described LED instruction dot matrix circuit；
The logic circuit being connected with described controller signals, described logic circuit is described defeated with described LED instruction dot matrix circuit
Go out end to be connected；
The power circuit being connected with described controller and described logic circuit, for supplying to described controller and described logic circuit
Electricity；
Be connected man-machine interaction touch screen with described controller, for providing the user editor and controlling the interface of operation；
The button being connected with described controller triggers circuit, provides for described controller and triggers signal.
2. a kind of enzyme mark microwell plate loading instruction control system as claimed in claim 1, it is characterised in that: this system also includes
Condition indication circuit, described condition indication circuit is connected with described controller, and described condition indication circuit includes or left behaviour
Make display lamp and lower or right tutorial light.
3. a kind of enzyme mark microwell plate loading instruction control system as claimed in claim 1, it is characterised in that: described logic circuit
Including some multiway analog switches and a bus switch, described multiway analog switch and described bus switch respectively with described control
Device signal connects.
4. a kind of enzyme mark microwell plate loading instruction control system as claimed in claim 1, it is characterised in that: described ignition mode
Including ignition mode one by one, row ignition mode and row ignition mode.
5. enzyme mark microwell plate loading instruction control system as claimed in claim 4 a kind of, it is characterised in that: described light one by one
Pattern includes with row pattern lightening LED lamp one by one and row mode lightening LED lamp one by one.
6. a kind of enzyme mark microwell plate loading instruction control system as claimed in claim 1, it is characterised in that: described LED indicates
The LED matrix of dot matrix circuit is 8*12 matrix or 16*24 matrix.
7. a kind of enzyme mark microwell plate loading instruction control system as claimed in claim 1, it is characterised in that: this system also includes
Foot switch control circuit, described foot switch control circuit is connected with described controller.
8. a kind of enzyme mark microwell plate loading instruction control system as claimed in claim 1, it is characterised in that: this system also includes
Peripheral circuit, described peripheral circuit is connected with described controller, and described peripheral circuit includes clock circuit, reset circuit and program
Download circuit.
9. an enzyme mark microwell plate loading indication control method, it is characterised in that specifically comprise the following steps that
S1, user is by the ignition mode in man-machine interaction touch screen selection control；
S2, user is according to ignition mode to the plate hole loading of enzyme mark microwell plate, and the every loading of user once, light LED and refer to by controller
Show the LED of current loading position in dot matrix circuit；
S3, user is according to ignition mode, and circulation step S2, successively to the plate hole loading of enzyme mark microwell plate, until it is micro-to complete enzyme mark
Orifice plate loading.
10. a kind of enzyme mark microwell plate loading indication control method as claimed in claim 8, it is characterised in that: also include S4, enzyme
After mark microwell plate completion of the sample, operating SR, all LED are turned off.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610514637.1A CN106066404A (en) | 2016-07-01 | 2016-07-01 | A kind of enzyme mark microwell plate loading instruction control system and control method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610514637.1A CN106066404A (en) | 2016-07-01 | 2016-07-01 | A kind of enzyme mark microwell plate loading instruction control system and control method |
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| CN106066404A true CN106066404A (en) | 2016-11-02 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610514637.1A Pending CN106066404A (en) | 2016-07-01 | 2016-07-01 | A kind of enzyme mark microwell plate loading instruction control system and control method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107315368A (en) * | 2017-06-29 | 2017-11-03 | 王效芬 | A kind of 96 holes auxiliary sample adding device |
| WO2019006778A1 (en) * | 2017-07-04 | 2019-01-10 | 王效芬 | 384-hole auxiliary sample injector |
| CN109583253A (en) * | 2018-11-06 | 2019-04-05 | 南华大学 | ELISA Plate positioning and information carrying means and method |
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| CN107315368A (en) * | 2017-06-29 | 2017-11-03 | 王效芬 | A kind of 96 holes auxiliary sample adding device |
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| CN109583253A (en) * | 2018-11-06 | 2019-04-05 | 南华大学 | ELISA Plate positioning and information carrying means and method |
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Application publication date: 20161102 |
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