CN106066325A - A kind of method of detection of alkaline phosphatase - Google Patents
A kind of method of detection of alkaline phosphatase Download PDFInfo
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- CN106066325A CN106066325A CN201610352426.2A CN201610352426A CN106066325A CN 106066325 A CN106066325 A CN 106066325A CN 201610352426 A CN201610352426 A CN 201610352426A CN 106066325 A CN106066325 A CN 106066325A
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- 102000002260 Alkaline Phosphatase Human genes 0.000 title claims abstract description 35
- 108020004774 Alkaline Phosphatase Proteins 0.000 title claims abstract description 35
- 238000001514 detection method Methods 0.000 title claims abstract description 29
- 238000000034 method Methods 0.000 title claims abstract description 21
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 30
- 235000010323 ascorbic acid Nutrition 0.000 claims abstract description 15
- 229960005070 ascorbic acid Drugs 0.000 claims abstract description 15
- 239000011668 ascorbic acid Substances 0.000 claims abstract description 15
- 150000003014 phosphoric acid esters Chemical class 0.000 claims abstract description 9
- 239000000243 solution Substances 0.000 claims description 49
- 238000002835 absorbance Methods 0.000 claims description 11
- 238000006243 chemical reaction Methods 0.000 claims description 9
- 239000011259 mixed solution Substances 0.000 claims description 7
- 239000007853 buffer solution Substances 0.000 claims description 6
- 229910000366 copper(II) sulfate Inorganic materials 0.000 claims description 6
- 230000004044 response Effects 0.000 claims description 6
- 230000003139 buffering effect Effects 0.000 claims 2
- 239000007788 liquid Substances 0.000 claims 2
- 230000008033 biological extinction Effects 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 6
- 108010001336 Horseradish Peroxidase Proteins 0.000 abstract description 3
- 238000004737 colorimetric analysis Methods 0.000 abstract description 2
- 230000009467 reduction Effects 0.000 abstract description 2
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 7
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 7
- 239000003513 alkali Substances 0.000 description 7
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 102000001554 Hemoglobins Human genes 0.000 description 2
- 108010054147 Hemoglobins Proteins 0.000 description 2
- 102000016943 Muramidase Human genes 0.000 description 2
- 108010014251 Muramidase Proteins 0.000 description 2
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 2
- 108090000190 Thrombin Proteins 0.000 description 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 229960000274 lysozyme Drugs 0.000 description 2
- 235000010335 lysozyme Nutrition 0.000 description 2
- 239000004325 lysozyme Substances 0.000 description 2
- 238000011896 sensitive detection Methods 0.000 description 2
- 229960004072 thrombin Drugs 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- GZCWLCBFPRFLKL-UHFFFAOYSA-N 1-prop-2-ynoxypropan-2-ol Chemical compound CC(O)COCC#C GZCWLCBFPRFLKL-UHFFFAOYSA-N 0.000 description 1
- 102000013563 Acid Phosphatase Human genes 0.000 description 1
- 108010051457 Acid Phosphatase Proteins 0.000 description 1
- 208000018084 Bone neoplasm Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 244000283207 Indigofera tinctoria Species 0.000 description 1
- 206010023129 Jaundice cholestatic Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 201000005267 Obstructive Jaundice Diseases 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000022131 cell cycle Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000005518 electrochemistry Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000003862 health status Effects 0.000 description 1
- 208000006454 hepatitis Diseases 0.000 description 1
- 231100000283 hepatitis Toxicity 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 208000019423 liver disease Diseases 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 208000005368 osteomalacia Diseases 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
A kind of method that the invention provides detection of alkaline phosphatase, utilizes alkaline phosphatase reduction ascorbic acid phosphoric acid esters AAP to generate ascorbic acid AA, and then ascorbic acid makes Cu2+It is reduced into Cu+, Cu+The activity of horseradish peroxidase can be suppressed, thus TMB H can not be made2O2Solution changes color, it is achieved that the detection of ALP.Compared with prior art, the present invention utilizes colorimetry to carry out the detection by quantitative of ALP, highly sensitive, and detection limit is low, and accuracy is high.
Description
Technical field
The invention belongs to photo chemistry technology field, a kind of method being specifically related to detection of alkaline phosphatase, build Cu2+With
HRP-TMB-H2O2Colorimetric System realizes the Sensitive Detection to alkali phosphatase.
Background technology
In recent years, the health status of the mankind increasingly receives publicity, and alkali phosphatase (ALP) is as a typical phosphoric acid
Monoesterase is one of important biomolecule mark of the multiple disease of the mankind, it can by diversified catalyzing hydrolysis and turn phosphoric acid,
Including some biomacromolecules and little molecule.Therefore, alkali phosphatase is at cell cycle, growth, the signal transduction of apoptosis and thin
The regulation process of intracellular plays critical effect.The exception of the ALP level in human body is the signal of multiple disease, especially relates to
And be that including skeletal diseases, (bone tumor is worn through the diagnosis index frequently as some diseases to liver and the flesh and blood ALP activity in clear
Lucky special sick, osteomalacia), hepatic disease (cancer, hepatitis, obstructive jaundice) etc..Although the research to ALP is relatively broad, but
Its different physiology and pathologic function illustrate the most completely.Recently, ALP is to the maintenance of intestinal environment stable state and protective effect also
Start to reveal.Therefore, the dynamic ALP activity to various biosystems makes further research and is necessary.
In view of their importance, the most different methods has been used for detecting ALP concentration in organism.With regard to ALP
For, there are fluorescence, electrochemistry, the research means such as chemiluminescence and serrs.Although in these methods
Some has higher sensitivity, but they great majority can not monitor enzymatic activity in real time.
Summary of the invention
A kind of method that it is an object of the invention to provide detection of alkaline phosphatase, builds Cu2+And HRP-TMB-H2O2Ratio
Color system realizes the Sensitive Detection to alkali phosphatase.The present invention utilizes alkaline phosphatase reduction ascorbic acid phosphoric acid esters AAP
Generating ascorbic acid AA, then ascorbic acid makes Cu2+It is reduced into Cu+, Cu+The activity of horseradish peroxidase can be suppressed, from
And TMB-H can not be made2O2Solution changes color, it is achieved that the detection of ALP.
The method of a kind of detection of alkaline phosphatase that the present invention provides, comprises the following steps:
A, the alkaline phosphatase solution of ascorbic acid phosphoric acid esters AAP solution and a series of Concentraton gradient is blended in PBS
In buffer solution, it is subsequently adding CuSO4Solution, reaction, obtain mixed solution；
B, respectively addition HRP solution in mixed solution prepared by step a, under room temperature after reaction, add TMB-H2O2Colour developing
Solution, detects its absorbance respectively, builds the linear relationship of alkaline phosphatase solution concentration and absorbance；
C, utilize linear relationship, obtain alkaline phosphatase solution concentration to be detected.
Further, the concentration of the alkaline phosphatase solution of a series of Concentraton gradient described in step a is: 0mU/mL,
10mU/mL, 20mU/mL, 40mU/mL, 60mU/mL, 80mU/mL, 100mU/mL and 120mU/mL.
In step a, the pH of PBS buffer solution is 5-9；Preferably pH=7.4.
Further, react described in step a, be specially at 20-25 DEG C, ALP and AAP response time 5-90min.
Further, step a particularly as follows:
By ascorbic acid phosphoric acid esters AAP solution and the alkali phosphatase of the 15 a series of Concentraton gradient of μ L of 10 μ L 1mg/mL
ALP solution is blended in the PBS buffer solution of 100mL pH=7.4, and Concentraton gradient is respectively 0mU/mL, 10mU/mL, 20mU/
ML, 40mU/mL, 60mU/mL, 80mU/mL, 100mU/mL and 120mU/mL, be then respectively adding 10 μ L, 100 μ g/mL CuSO4
Solution, at room temperature reacts 40min；
Step b is particularly as follows: add the HRP solution room of 50 μ L 10ng/mL respectively in above-mentioned steps a gained mixed solution
After the lower reaction 20min of temperature, it is eventually adding the TMB-H of 100 μ L 8.3mM2O2Chromophoric solution, detects its absorbance respectively, builds alkali
Acid phosphatase ALP solution concentration and the linear relationship of absorbance.
Before step a, by the alkaline phosphatase bought and ascorbic acid AAP secondary water dissolution, it is made into respectively
The alkaline phosphatase solution of 0.1U/mL and 1mg/mL ascorbic acid phosphoric acid esters AAP solution, prepare 100 μ g/mL the most respectively
CuSO4Solution, 10ng/mL HRP and 8.3mM TMB-H2O2Solution, and save backup at 4 DEG C.
The present invention is based on Cu+The activity of HRP can be suppressed, so that TMB-H2O2Solution colour produces change, builds colorimetric
System.In the solution of detection ALP, the constant indigo plant of solution colour, the most thin out along with color, absorbance is gradually reduced；Based on
Cu2+The detection to ALP is successfully realized with HRP Colorimetric System.Owing to the depth of color and the concentration of ALP are relevant, dense along with ALP
Degree increases, and color gradually becomes shallower as until colourless, and therefore this Colorimetric System can carry out detection by quantitative to the ALP of variable concentrations.
Compared with prior art, the present invention utilizes colorimetry to carry out the detection by quantitative of ALP, highly sensitive, and detection limit is low, and
And accuracy is high, detection limit is 0.9966.
Accompanying drawing explanation
Fig. 1 is based on Cu2+Colorimetric System colorimetric determination alkaline phosphatase is prepared with horseradish peroxidase HRP
Experimental principle figure；
Fig. 2 is the feasibility analysis figure of detection ALP；ALP exists and does not deposit ultraviolet spectrogram in both cases, wherein
For there is ALP in a, b is not for exist ALP；
Fig. 3 A be pH this is tested affect figure；Controlling the pH of whole system, pH is respectively 5,6,7.4,8 and 9；
Fig. 3 B be the response time this is tested affect figure；Controlling the response time of AAP and ALP, the response time is respectively
5min, 10min, 20min, 40min, 60min and 90min；
Fig. 3 C be reaction temperature this is tested affect figure；Control the reaction temperature of AAP and ALP, control at 20 DEG C respectively,
25 DEG C, 30 DEG C, 37 DEG C, 40 DEG C, 45 DEG C and 50 DEG C；
Fig. 4 A be the concentration of ALP solution be 0mU/mL, 10mU/mL, 20mU/mL, 40mU/mL, 60mU/mL, 80mU/mL,
The ultraviolet spectrogram of the ALP of 100mU/mL and 120mU/mL；
Fig. 4 B is the linear relationship chart of ALP；
Fig. 5 is the Colorimetric System selectivity pair to tetra-kinds of protein of Hemoglobin, thrombin, lysozyme and ALP
Than figure；
Detailed description of the invention
Embodiment 1
A kind of method of detection of alkaline phosphatase, comprises the following steps:
By the alkaline phosphatase bought and ascorbic acid AAP secondary water dissolution, it is made into the alkalescence of 0.1U/mL respectively
Phosphatase ALP solution and 1mg/mL ascorbic acid phosphoric acid esters AAP solution, prepare 100 μ g/mL CuSO the most respectively4Solution,
10ng/mL HRP and 8.3mM TMB-H2O2Solution, and save backup at 4 DEG C.
A, by the ascorbic acid phosphoric acid esters AAP solution of 10 μ L 1mg/mL and the alkaline phosphatase of the 15 a series of Concentraton gradient of μ L
Enzyme ALP solution is blended in 100mL PBS buffer solution (pH=7.4), and Concentraton gradient is respectively 0mU/mL, 10mU/mL,
20mU/mL, 40mU/mL, 60mU/mL, 80mU/mL, 100mU/mL and 120mU/mL, be then respectively adding 10 μ L, 100 μ g/mL
CuSO4Solution, at room temperature reacts 40min；
B, add in above-mentioned steps a gained mixed solution respectively and react under the HRP solution room temperature of 50 μ L 10ng/mL
After 20min, it is eventually adding the TMB-H of 100 μ L 8.3mM2O2Chromophoric solution, detects its absorbance respectively, builds alkali phosphatase
ALP solution concentration and the linear relationship of absorbance.As described in Fig. 4 A, 4B.
C, utilize linear relationship, obtain alkaline phosphatase solution concentration to be detected.
Embodiment 2
A kind of method of detection of alkaline phosphatase, the pH of PBS buffer solution is respectively 5,6,7.4,8 and 9, other and enforcement
Example 1 operates identical, and result is as shown in Figure 3A.Embodiment 3
A kind of method of detection of alkaline phosphatase, in step a, the response time is 5min, 10min, 20min, 40min,
60min and 90min, other operate identical with embodiment 1, and result is as shown in Figure 3 B.
Embodiment 4
A kind of method of detection of alkaline phosphatase, in step a, reaction temperature is 20 DEG C, 25 DEG C, 30 DEG C, 37 DEG C, 40 DEG C, 45
DEG C and 50 DEG C, other operate identical with embodiment 1, and result is as shown in Figure 3 C.
Embodiment 5 selectivity is tested
By 4 group of 10 μ L, the AAP of 1mg/mL is separately added into 120mU/mL ALP, 1 μM of thrombin, 10 μMs
Hemoglobin, and after 8kU/mL (29mM) lysozyme reacts 40min at 37 DEG C, it is separately added into 50 μ L, 10ng/mL's
HRP reacts 20min, is eventually adding 100 μ L, the TMB of 8.3mM, observes the color of solution；Separately do one group of blank experiment, be not added with
ALP, operates same as described above.
Absorbance result is as shown in Figure 5, it is seen that the method is higher to the selectivity of the detection of ALP.
Claims (6)
1. the method for a detection of alkaline phosphatase, it is characterised in that the method for described detection of alkaline phosphatase includes following step
Rapid:
A, the alkaline phosphatase solution of ascorbic acid phosphoric acid esters AAP solution and a series of Concentraton gradient is blended in PBS buffering
In solution, it is subsequently adding CuSO4Solution, reaction, obtain mixed solution；
B, respectively addition HRP solution in mixed solution prepared by step a, under room temperature after reaction, add TMB-H2O2Develop the color molten
Liquid, detects its absorbance respectively, builds the linear relationship of alkaline phosphatase solution concentration and absorbance；
C, utilize linear relationship, obtain alkaline phosphatase solution concentration to be detected.
The method of detection of alkaline phosphatase the most according to claim 1, it is characterised in that a series of dense described in step a
The concentration of the alkaline phosphatase solution of degree gradient is: 0mU/mL, 10mU/mL, 20mU/mL, 40mU/mL, 60mU/mL,
80mU/mL, 100mU/mL and 120mU/mL.
The method of detection of alkaline phosphatase the most according to claim 1 and 2, it is characterised in that in step a, PBS buffering is molten
The pH of liquid is 5-9.
The method of detection of alkaline phosphatase the most according to claim 1 and 2, it is characterised in that react described in step a,
It is specially at 20-25 DEG C, ALP and AAP response time 5-90min.
The method of detection of alkaline phosphatase the most according to claim 1 and 2, it is characterised in that step a is particularly as follows: by 10 μ
The ascorbic acid phosphoric acid esters AAP solution of L 1mg/mL and the alkaline phosphatase solution of the 15 a series of Concentraton gradient of μ L are blended in
In the PBS buffer solution of 100mL pH=7.4, Concentraton gradient is respectively 0mU/mL, 10mU/mL, 20mU/mL, 40mU/mL,
60mU/mL, 80mU/mL, 100mU/mL and 120mU/mL, be then respectively adding 10 μ L, 100 μ g/mL CuSO4Solution, in room temperature
Lower reaction 40min.
The method of detection of alkaline phosphatase the most according to claim 1 and 2, it is characterised in that step b is particularly as follows: distinguish
Above-mentioned steps a gained mixed solution adds after reacting 20min under the HRP solution room temperature of 50 μ L 10ng/mL, be eventually adding
The TMB-H of 100 μ L 8.3mM2O2Chromophoric solution, detects its absorbance respectively, builds alkaline phosphatase solution concentration and extinction
The linear relationship of degree.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107084938A (en) * | 2017-05-01 | 2017-08-22 | 福建医科大学 | The alkaline phosphatase assay method of oxidizing ferment is simulated based on chitosan platinum |
| CN107238598A (en) * | 2017-05-01 | 2017-10-10 | 福建医科大学 | The Assay of acid phosphatase content method of oxidizing ferment is simulated based on chitosan platinum |
| CN107557431A (en) * | 2017-07-18 | 2018-01-09 | 天津大学 | A kind of kit of Visual retrieval S1 nucleases |
| CN108896506A (en) * | 2018-07-16 | 2018-11-27 | 济南大学 | The method of detection of alkaline phosphatase activity and Inhibitors of Alkaline Phosphatase concentration |
| CN109211821A (en) * | 2018-11-28 | 2019-01-15 | 安徽师范大学 | A kind of detection method of ascorbic acid |
| CN109668881A (en) * | 2019-01-31 | 2019-04-23 | 湖南大学 | Alkaline phosphatase portable detection reagent box and its application based on temperature change |
| CN110777189A (en) * | 2019-10-09 | 2020-02-11 | 天津大学 | Method for determining activity of alkaline phosphatase in activated sludge |
| CN111220608A (en) * | 2020-02-05 | 2020-06-02 | 江苏大学 | CoO based on vulcanization modificationxColorimetric detection method for alkaline phosphatase activity |
| CN111220609A (en) * | 2020-02-05 | 2020-06-02 | 江苏大学 | Based on CeVO4Colorimetric detection method of alkaline phosphatase Activity |
| CN111239094A (en) * | 2020-03-13 | 2020-06-05 | 河南中医药大学 | Sensitive detection method of alkaline phosphatase |
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Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107084938B (en) * | 2017-05-01 | 2020-02-28 | 福建医科大学 | Alkaline phosphatase determination method based on chitosan-platinum mimic oxidase |
| CN107238598A (en) * | 2017-05-01 | 2017-10-10 | 福建医科大学 | The Assay of acid phosphatase content method of oxidizing ferment is simulated based on chitosan platinum |
| CN107084938A (en) * | 2017-05-01 | 2017-08-22 | 福建医科大学 | The alkaline phosphatase assay method of oxidizing ferment is simulated based on chitosan platinum |
| CN107557431A (en) * | 2017-07-18 | 2018-01-09 | 天津大学 | A kind of kit of Visual retrieval S1 nucleases |
| CN108896506A (en) * | 2018-07-16 | 2018-11-27 | 济南大学 | The method of detection of alkaline phosphatase activity and Inhibitors of Alkaline Phosphatase concentration |
| CN108896506B (en) * | 2018-07-16 | 2020-10-27 | 济南大学 | Method for detecting alkaline phosphatase activity and concentration of alkaline phosphatase inhibitor |
| CN109211821A (en) * | 2018-11-28 | 2019-01-15 | 安徽师范大学 | A kind of detection method of ascorbic acid |
| CN109668881B (en) * | 2019-01-31 | 2020-06-16 | 湖南大学 | Portable alkaline phosphatase detection kit based on temperature change and application thereof |
| CN109668881A (en) * | 2019-01-31 | 2019-04-23 | 湖南大学 | Alkaline phosphatase portable detection reagent box and its application based on temperature change |
| CN110777189A (en) * | 2019-10-09 | 2020-02-11 | 天津大学 | Method for determining activity of alkaline phosphatase in activated sludge |
| CN111220608A (en) * | 2020-02-05 | 2020-06-02 | 江苏大学 | CoO based on vulcanization modificationxColorimetric detection method for alkaline phosphatase activity |
| CN111220609A (en) * | 2020-02-05 | 2020-06-02 | 江苏大学 | Based on CeVO4Colorimetric detection method of alkaline phosphatase Activity |
| CN111220608B (en) * | 2020-02-05 | 2022-02-15 | 江苏大学 | CoO based on vulcanization modificationxColorimetric detection method for alkaline phosphatase activity |
| CN111220609B (en) * | 2020-02-05 | 2022-04-26 | 江苏大学 | Based on CeVO4Colorimetric detection method of alkaline phosphatase Activity |
| CN111239094A (en) * | 2020-03-13 | 2020-06-05 | 河南中医药大学 | Sensitive detection method of alkaline phosphatase |
| CN111239094B (en) * | 2020-03-13 | 2022-08-26 | 河南中医药大学 | Sensitive detection method of alkaline phosphatase |
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