CN106053860B - A kind of anti-gyneduct hormone sampling kit and sampling and extracting method - Google Patents
A kind of anti-gyneduct hormone sampling kit and sampling and extracting method Download PDFInfo
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- CN106053860B CN106053860B CN201610634942.4A CN201610634942A CN106053860B CN 106053860 B CN106053860 B CN 106053860B CN 201610634942 A CN201610634942 A CN 201610634942A CN 106053860 B CN106053860 B CN 106053860B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/14—Devices for taking samples of blood ; Measuring characteristics of blood in vivo, e.g. gas concentration within the blood, pH-value of blood
- A61B5/1405—Devices for taking blood samples
- A61B5/1411—Devices for taking blood samples by percutaneous method, e.g. by lancet
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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Abstract
The present invention relates to a kind of anti-gyneduct hormone sampling kit and sampling and extracting methods, wherein cotton swab, disposable ending blood taking needle, sample acquisition card and sampling filter paper are equipped in sampling kit；It includes information registration area, bar code area and filter paper preserving chamber that sample, which acquires card,；The intermediate region of sampling filter paper sets there are two the position for limiting sample acquisition and definitely defines the circle of required sample collection capacity.The present invention uses kit, the method for sampling and extracting method, oligemia is acquired when measuring anti-gyneduct hormone from detected person to 40ul, mitigate pain when detected person acquires sample significantly, increases the acceptance level that women fights gyneduct hormone determination.What anti-gyneduct hormone sampling kit of the invention can be convenient is completed inside of the house by examinee, can not only mitigate medical institution personnel pressure, the area for also facilitating the medical technology such as vast remote mountain areas, rural area to fall behind is promoted the use of.
Description
Technical field
The present invention relates to samplings used in the method and this method of a kind of finger tip blood acquisition sample method and sample extraction
Kit.Institute's sample thief is mainly used in Protein Detection field, the application of especially anti-gyneduct hormone determination.
Background technique
" anti-gyneduct hormone (anti-Mullerianhormone, AMH), also referred to as gyneduct mortifier
(Mullerian inhibiting substance, MIS), is one of member of transforming growth actor βfamily, mainly by small
The gonad granulocyte secretion of ovarian follicle before ovarian follicle and sinus.There is studies have shown that serum AMH concentration to can reflect the big of primordial follicle library
It is small, it can be as the marker of Ovary reserve.In the women of normal menstrual cycle, serum AMH in the entire menstrual cycle
It is horizontal to maintain more stable expression, therefore the detection of AMH is not limited by the menstrual cycle, be detected at any time without significant fluctuation, this
Pay close attention to it by clinical examination subject, currently, serum AMH Protein Detection and as evaluation Ovary reserve and ovary
Reactive index has been declared United States Patent (USP) (on July 10th, 2007) and European patent (on August 11st, 2007) respectively and has been obtained
It must ratify.
Data shows that conventional AMH detection method is that enzyme-linked immunization AMH detection kit detects experimenter's serum at present
Middle AMH albumen, since enzyme-linked immunization detects protein techniques because its development time is long, technology maturation is stable and is answered extensively
With, but AMH enzyme-linked immunization also has a shortcoming of its own, the sample of AMH kit detection be venous blood sample or
Person's serum sample, since AMH enzyme linked immunosorbent detection needs the personnel of profession, professional equipment that could complete detection work,
In practical application, after the completion of the blood sample collection of detected person, it is necessary to by freezen protective, then by all samples being collected into
This concentration is transported to biology laboratory or medical testing agency to carry out AMH measurement.During this, if Sample preservation or
When transport, deal with improperly easily cause false negative or false positive results to even result in sample entirely ineffective, and since it is desired that blood
The acquisition of liquid sample, so must have healthcare participation, these shortcomings not only greatly increase enzyme linked immunological
RNA isolation kit detects the testing cost of AMH, and brings biggish pain to detected person, and some women is made to be unwilling to receive
AMH measurement, is not easy to the popularization and development of this technology."
In consideration of it, providing a kind of anti-gyneduct hormone sampling kit and sampling and extracting method is that the present invention to be ground
The project studied carefully.
Summary of the invention
The main purpose of the present invention is to provide a kind of anti-gyneduct hormones to sample kit and sampling and extracting method,
To overcome existing etc. disadvantage.
To realize that aforementioned invention purpose, the technical solution adopted by the present invention include: a kind of anti-gyneduct hormone sampling examination
Agent box, including being equipped with cotton swab, disposable ending blood taking needle, sample acquisition card and sampling filter paper in the sampling kit；
The sample acquisition card includes an information registration area, a bar code area and a filter paper preserving chamber；The letter
Breath registration area is for registering by the information of sampler；The bar code area is equipped with bar code；The filter paper preserving chamber is for saving
The sampling filter paper, the filter paper preserving chamber include a sample application region and a protecting cover, and the sample application region is acquired set on sample
On card, and it is located at the fringe region of sample acquisition card, the protecting cover is a flake structure, and one end of the flake structure rotates
It is connected in a side of the sample acquisition card, and the flake structure can be on the basis of the side, relative sample is adopted
Collection card is turned down to lid and is closed in the top of sample application region, and the flake structure is equipped with and pastes portion, this pastes portion can will be described
Flake structure is pasted on sample capture card, so that sampling filter paper is stored in the sample application region of filter paper preserving chamber；
A window to match with sample application region is offered on the flake structure, when flake structure lid is closed in sample application region
When top, the window is aligned with the sample application region；
The intermediate region of the sampling filter paper sets there are two the position for limiting sample acquisition and definitely defines required
The circle of sample collection capacity, length direction parallel arrangement of two circles along sampling filter paper.
As a further improvement of the present invention, the information registration area is for registering by the information of sampler.
As a further improvement of the present invention, the flake structure, which is equipped with, pastes sealing strip positioned at upper end and is located at two sides
Paste sealing strip.
As a further improvement of the present invention, the width of the sampling filter paper is 1-2 millimeters smaller than the width of filter paper preserving chamber.
To realize that aforementioned invention purpose, the technical solution adopted by the present invention include: using the anti-gyneduct hormone
Kit is sampled, is operated according to the following steps:
Step 1 takes a sample acquisition card, conscientiously, clearly fills in all information of sample capture card on piece；
The protecting cover of sample acquisition card is turned down and covers conjunction on the sample application region by step 2；
The protecting cover is located at the pasting after sealing strip is attached to sample capture card on piece of upper end by step 3 so that protecting cover and
The penetrating channel in a both ends is formed between sample application region；
Step 4 will sample filter paper in the channel both ends insertion filter paper preserving chamber between protecting cover and sample application region；
Step 5 closes the channel both ends with the sealing strip of pasting of two sides, sampling filter paper can be fixed on filter paper guarantor
Deposit interior；
Step 6 at alcohol cotton stick wiping blood sampling (two sections of finger tip), and waits alcohol to be completely dried；
Step 7 punctures skin at blood sampling with disposable blood taking needle；
Step 8 touches the sampling filter paper on paper jam after drop of blood is formed, and allows blood full of two circles；
Step 9, with the skin at the cleaning blood sampling of dry cotton swab；
Step 10, the sample capture card and desiccant that collect sample is sealed to be placed in valve bag together saves.
As a further improvement of the present invention, it when sampling weather cold, puts his hand into warm bag or warm water 2-4 minutes.
As a further improvement of the present invention, when sampling, punctured at blood sampling after skin with disposable blood taking needle, can from refer to
Finger is gently squeezed to finger tip.
As a further improvement of the present invention, collected sample can pass through bar code or information preservation card approach management
Sample message.
To realize aforementioned invention purpose, the technical solution adopted by the present invention includes: a kind of anti-gyneduct hormone extraction side
Method is used the acquisition sample sampled with top sampling method, is operated according to the following steps:
The first step is punched the blood cake for sampling filter paper using paper sheet punching machine；
The blood cake sample being drilled is put into centrifuge tube, and has marked bar code number by second step, the bar code label and institute
The bar code for stating bar code area in sampling kit is corresponding；
Third step is added pre-configured sample buffer in the centrifuge tube for being packed into blood cake sample, and makes this
The molten sample buffer of phosphate-buffered salt did not had the blood cake sample just；
The centrifuge tube that sample buffer is added in third step is placed in progress room temperature concussion in shaking table mechanism by the 4th step；
The centrifuge tube for passing through room temperature concussion in 4th step is placed in centrifugal mechanism and is centrifuged by the 5th step, allows blood cake sample
Bottom is arrived in this full centrifugation；
6th step is put sample well, sequentially with use to be detected.
As a further improvement of the present invention, prefabricated sample buffer is configured using following steps:
Deionized water and trishydroxymethylaminomethane are configured to obtain by step S1 according to 1000: 12.1 mass ratio
0.1mol/L tris solution；
36~37% concentrated hydrochloric acid and water are mixed according to 9: 1000 volume ratio and shake up to obtain hydrochloric acid solution by step S2,
Closed storage；
Step S3 presses hydrochloric acid solution obtained in tris solution obtained in step S1 and step S2
It is mixed according to 50: 44.7 volume ratio；
The PH of mixed liquor in step S4, measuring process S3, and after adjusting the PH to 7.2 of the mixed liquor, by mixed liquor constant volume
Trishydroxymethylaminomethane-hydrochloric acid solution is obtained to 100ml；
Step S5 adds Tween-20 according to matching in direction trishydroxymethylaminomethane-hydrochloric acid solution in below table
Solution, fetal calf serum, NaCl solid and NaN3 solid；Wherein, the table are as follows:
Due to the above technical solutions, the present invention has the following advantages over the prior art:
1, oligemia is acquired when measuring anti-gyneduct hormone using the present invention from detected person to 40ul, significantly
Mitigate pain when detected person acquires sample, increases the acceptance level that women fights gyneduct hormone determination.Tradition is adopted
Quadrat method is the direct venous blood for extracting detected person, and blood sampling volume is greater than 1ml, portion bigger to detected person's bring pain
Therefore point women can fear the health examination for doing this intermediate item.And when using this method acquisition sample, only need 40 μ l or so finger tip
Blood, entire collection process is very of short duration, and larger pain will not be caused to detected person, therefore is easier that women is made to receive anti-seedling
The measurement of Le Shi pipe hormone.
2, sample acquires more convenient when measuring anti-gyneduct hormone using the present invention, and the scope of application is more extensive.Traditional
Sample must be adopted in special environment such as hospital, testing agencies by the mode that medical staff is detected person's venous blood samples
Collection；And when using this method acquisition sample, due to not being related to venous blood collection operation, it may be convenient to inside of the house by examinee
It completes, detected person no longer needs to medical institutions' queuing and waits to see the doctor, and can not only mitigate medical institution personnel pressure, also facilitates vast partially
The area that the medical technology such as distant mountain area, rural area fall behind is promoted the use of, and has greater significance to universal fitness-for-all physical examination.
3, sample needed for measuring anti-gyneduct hormone using the present invention saves, transportation cost is lower.Traditional detection is used
Sample is whole blood or blood serum sample, both for liquid sample, therefore either saves or transports, is required to the cold of profession
Freeze equipment, and needs memory space very big, it, then must be by the cold chain transportation equipment or fortune of profession if necessary to transport sample
Defeated system.The collected sample of this method is to save carrier, volume very little, and sample is with dry solid state with card
In the presence of so even being that under normal temperature conditions, also the long period can keep sample activity.Moreover, the collected sample of this method
It can directly be transported using common express delivery or logistics system, required cost is lower, applicability is wider.
4, anti-gyneduct hormone sample extraction is measured, using more convenient using the present invention, effect is more preferable.With our company
Special sample extraction buffer and method extract the AMH ingredient on dried blood spot sample, and operating process is simple, and that extracts is effective
Component content is high, and detection effect is good, reaches 0.99 with the degree of correlation of venous blood sample.
5, anti-gyneduct hormone is measured using the present invention, collected sample managing is more convenient.It is acquired using the present invention
The sample arrived can save carrier and be reserved with mark by the convenient and fast management sample information of the approach such as bar code, information preservation card, sample
Infuse the position of sample message, be particularly suitable for that sample source is more dispersed or sample size it is huge when centralized management.
6, anti-gyneduct hormone is measured using the present invention, reduces sample and keeps sample management cost.It is obtained using this method
Sample, after finishing anti-gyneduct hormone determination, sample more traditional blood sample that keeps sample keeps sample, and management gets up not only take up
Resource is less, and the effective information that can retain is more, and the holding time is longer.
In conclusion the present invention is compared with the traditional method, have clear advantage: 1. samples only need 40ul finger tip blood, greatly
Pain when examinee's sampling is alleviated greatly；2. entire sampling process fast and easy is not necessarily to health care professional, applicable various
Occasion；3. sample storage, convenient transportation can all carry out at normal temperature.
Detailed description of the invention
Attached drawing described here is only used for task of explanation, and is not intended to limit model disclosed in the present application in any way
It encloses.In addition, shape and proportional sizes of each component in figure etc. are only schematical, it is used to help the understanding to the application, and
It is not the specific shape and proportional sizes for limiting each component of the application.Those skilled in the art, can under teachings of the present application
Implement the application to select various possible shapes and proportional sizes as the case may be.In the accompanying drawings:
Attached drawing 1 is the structural schematic diagram of the specific embodiment of the invention one；
Attached drawing 2 is the use state diagram of the specific embodiment of the invention one.
In the figures above: 1, sample acquires card；2, information registration area；3, bar code area；4, filter paper preserving chamber；40, add
Sample region；41, protecting cover；42, window；43, sealing is pasted in upper end；44, sealing strip is pasted in two sides；5, filter paper is sampled；50, circle
Circle.
Specific embodiment
The application is described in detail below with reference to specific embodiment shown in the drawings.But these embodiments are simultaneously
The application is not limited, structure that those skilled in the art are made according to these embodiments, method or functionally
Transformation is all contained in the protection scope of the application.
In each diagram of the application, for the ease of illustration, structure or partial certain sizes can be relative to other knots
Therefore structure or partial enlargement are only used for the basic structure of the theme of diagram the application.
A kind of embodiment one: anti-gyneduct hormone sampling kit
It is equipped with cotton swab, disposable ending blood taking needle, sample referring to attached drawing 1-2, in the sampling kit and acquires card 1
And sampling filter paper 5.
The sample acquisition card 1 includes an information registration area 2, a bar code area 3 and a filter paper preserving chamber 4；Institute
Information registration area 1 is stated for registering by the information of sampler.
The bar code area 2 is equipped with bar code；The filter paper preserving chamber 4 is for saving the sampling filter paper 5, the filter
Paper preserving chamber 4 includes a sample application region 40 and a protecting cover 41, and the sample application region 40 is set on sample acquisition card 1, and is located at
Sample acquires the fringe region of card 1, and the protecting cover 41 is a flake structure, and one end of the flake structure is rotatably connected on described
Sample acquires in a side of card 1, and the flake structure can be on the basis of the side, and relative sample acquisition card 1 turns over
It is folded to lid to close in the top of sample application region 40, the protecting cover 41 is equipped with and pastes portion, this is pasted portion and can stick the protecting cover 41
It is attached on sample capture card 1, so that sampling filter paper 5 is stored in the sample application region of filter paper preserving chamber.
A window 42 to match with sample application region 40 is offered on the protecting cover 41, when the lid of protecting cover 41 is closed in sample application region
When 40 top, the window 42 is aligned with the sample application region 40.
Wherein, the intermediate region of the sampling filter paper 5 sets there are two the position for limiting sample acquisition and definitely defines
The circle 50 of required sample collection capacity, length direction parallel arrangement of two circles 50 along sampling filter paper 5.
In the present embodiment, the size for sampling filter paper 5 is 5mm or more more major than 42 length and width of sample-adding window, and sticks
It is affixed on 50 back side of sampling window, filter paper 5 is sampled in 42 middle position of sampling window and is decorated with the circle 50 of 2 diameter 8mm, institute
Circle 50 is stated for limiting sample application region 40 and assessment sampling quantity.
In the present embodiment, the information registration area 2 is for registering by the information of sampler.The flake structure is equipped with position
Sealing 43 is pasted in upper end and pastes sealing strip 44 positioned at two sides.The width of the sampling filter paper 5 is than filter paper preserving chamber 4
Width is 1-2 millimeters small.
Embodiment 2: a kind of anti-gyneduct hormone method of sampling samples reagent using the anti-gyneduct hormone
Box is operated according to the following steps:
Step 1 takes a sample acquisition card 1, conscientiously, clearly fills in all information on sample acquisition card 1；
The protecting cover 41 of sample acquisition card 1 is turned down and covers conjunction on the sample application region 40 by step 2；
Step 3, by the protecting cover 41 be located at upper end paste sealing 43 be attached to sample acquisition card 1 on after so that shield
The penetrating channel in a both ends is formed between lid 41 and sample application region 40；
Step 4 will sample channel both ends of the filter paper 5 between protecting cover 41 and sample application region 40 and be inserted into filter paper preserving chamber 4
In；
Step 5 closes the channel both ends with the sealing strip 44 of pasting of two sides, sampling filter paper 5 can be fixed on filter paper
In preserving chamber 4:
Step 6 at alcohol cotton stick wiping blood sampling (two sections of finger tip), and waits alcohol to be completely dried；
Step 7, punctures skin at blood sampling with disposable blood taking needle, punctures skin (flicking at blood sampling with disposable blood taking needle
Blood taking needle, syringe needle meeting automatic spring)；
Step 8 touches the sampling filter paper 5 on paper jam after drop of blood is formed, and allows blood full of two circles 50；
Step 9, with the skin at the cleaning blood sampling of dry cotton swab；
Step 10, the sample capture card 1 and desiccant that collect sample is sealed to be placed in valve bag together saves.
Further, it when sampling weather cold, puts his hand into warm bag or warm water 2-4 minutes.
Further, it when sampling, is punctured at blood sampling after skin with disposable blood taking needle, it can be from referring to gently being squeezed to finger tip
Finger.
Further, the approach management sample information that collected sample can be registered by bar code or information, and
To qualified sampling card information, logged in；Sample message includes bar code, name, gender, age, mobile phone, report sender
Formula etc. forms excel table；It needs to log in and check during information login.
After collecting sample, sample acquisition card 1 should seal together with desiccant to be placed in valve bag, contains sample
Capture card freezing conditions under can save for a long time, transport then can choose normal temperature condition.
Embodiment 3: a kind of anti-gyneduct hormone extracting method uses the acquisition sample sampled with top sampling method,
It is operated according to the following steps:
The blood cake 50 for sampling filter paper 5 is punched using paper sheet punching machine, in the step, is with aperture by the first step
The paper sheet punching machine of 6.0mm will be punched using the blood cake in filter paper 5, sample this need to meet it is claimed below:
A. 50 diameter of blood cake circle taken that drills is 6.0mm；
B. the positive and negative color of blood cake circle 50 is uniform, and without white portion；
The sample being drilled is put into the centrifuge tube of 2.0ml, and corresponds and has marked bar code number.It is needed during this
Review；
The blood cake sample being drilled is put into centrifuge tube, and has marked bar code number by second step, the bar code label and institute
The bar code for stating bar code area in sampling kit is corresponding；
Third step is added pre-configured sample buffer in the centrifuge tube for being packed into blood cake sample, and makes this
Sample buffer did not had the blood cake sample just, in this step, therefore, to assure that for blood cake piece all in centrifuge tube bottom of the tube, sample is slow
Fliud flushing there was not blood cake piece, otherwise gently played centrifuge tube with finger；Do not had blood cake to buffer；
The centrifuge tube that sample buffer is added in third step is placed in progress room temperature concussion in shaking table mechanism by the 4th step, this
In step, overnight using 37 DEG C of concussions, specially by the centrifuge tube in the step on shaking table, parameter be set as 37 DEG C,
300rpmin revolution, overnight (12 hours)；
The centrifuge tube for passing through room temperature concussion in 4th step is placed in centrifugal mechanism and is centrifuged by the 5th step, allows blood cake sample
Bottom is arrived in this full centrifugation, specially uses low speed (< 500rpnin) to be centrifuged respectively in 37 DEG C of overnight centrifuge tube samples of concussion
1min allows the sample solution of wall built-up be centrifuged entirely to bottom；
6th step is put sample well, sequentially with use to be detected.
Wherein, in the present embodiment, the prefabricated sample buffer is configured using following steps:
Deionized water and trishydroxymethylaminomethane are configured to obtain by step S1 according to 1000: 12.1 mass ratio
0.1mol/L tris solution；
36~37% concentrated hydrochloric acid and water are mixed according to 9: 1000 volume ratio and shake up to obtain hydrochloric acid solution by step S2,
Closed storage；
Step S3 presses hydrochloric acid solution obtained in tris solution obtained in step S1 and step S2
It is mixed according to 50: 44.7 volume ratio；
The PH of mixed liquor in step S4, measuring process S3, and after adjusting liquid PH to 7.2, by liquid capacity-fixed to 100ml
Obtain trishydroxymethylaminomethane-hydrochloric acid solution；
Step S5 adds Tween-20 according to matching in direction trishydroxymethylaminomethane-hydrochloric acid solution in below table
Solution, fetal calf serum, NaCl solid and NaN3 solid；Wherein, the table are as follows:
It in the present embodiment, before extraction, needs to confirm sample capture card 1, sample capture card 1 needs to meet following
It is required that:
A. the blood sample on the sampling filter paper 5 on sample acquisition card 1 has been dried；
B. blood cake range is greater than the marked circle 50 of sampling card, and without white portion, (i.e. tow sides are all saturated with tow sides
Sample)；If on sample acquisition card 1 two blood cakes only one meet the requirements, selection is wherein met the requirements when being punched
Blood cake carry out punching sampling；
C. two blood cakes on sample acquisition card 1 cannot be overlapped；Overlapping can cause prohibited data detection true.
It should be noted that "front", "rear" "upper" "lower" is retouched with reference to the direction of Fig. 1 of the present invention in above scheme
State, "front", "rear", " left side " and " right side " be all it is opposite, using direction shown in Fig. 1 as reference.
It should be noted that it can be directly to separately when an element is considered as " connection " another element
One element may be simultaneously present centering elements.Unless otherwise defined, all technologies used herein and section are academic
Language is identical as the normally understood meaning of the those skilled in the art for belonging to the application.Herein in the description of the present application
Used term is only for the purpose of describing specific embodiments, it is not intended that in limitation the application.It is used herein
Term "and/or" include any and all combinations of one or more related listed items.
It should be noted that in the description of the present application, in the description of the present application, unless otherwise indicated, " multiples' "
It is meant that two or more.
Here the combination of element, ingredient, component or step is described using term "comprising" or " comprising " it is also contemplated that
Substantially the embodiment being made of these elements, ingredient, component or step.Here by using term " can with ", it is intended to illustrate
Described any attribute that " can with " includes all is optional.
Multiple element, ingredient, component or step can be provided by single integrated component, ingredient, component or step.Optionally
Ground, single integrated component, ingredient, component or step can be divided into multiple element, ingredient, component or the step of separation.It is used to
The open "a" or "an" for describing element, ingredient, component or step is not said to exclude other elements, ingredient, component
Or step.
It should be understood that above description is to illustrate rather than to be limited.By reading above-mentioned retouch
It states, many embodiments and many applications except provided example all will be aobvious and easy for a person skilled in the art
See.Therefore, the range of this introduction should not be determined referring to foregoing description, but should referring to preceding claims and this
The full scope of the equivalent that a little claims are possessed determines.For comprehensive purpose, all articles and with reference to including special
The disclosure of benefit application and bulletin is all by reference to being incorporated herein.Theme disclosed herein is omitted in preceding claims
Any aspect is not intended to abandon the body matter, also should not be considered as applicant the theme is not thought of as it is disclosed
Apply for a part of theme.
The series of detailed descriptions listed above only for the application feasible embodiment specifically
Bright, they are not the protection scope to limit the application, all without departing from equivalent implementations made by the application skill spirit
Or change should be included within the scope of protection of this application.
Claims (4)
1. a kind of anti-gyneduct hormone samples kit, it is characterised in that: be equipped with cotton swab, primary in the sampling kit
Property ending blood taking needle, sample acquisition card and sampling filter paper；
The sample acquisition card includes an information registration area, a bar code area and a filter paper preserving chamber；The information is stepped on
Note area is for registering by the information of sampler；The bar code area is equipped with bar code；The filter paper preserving chamber is described for saving
Filter paper is sampled, the filter paper preserving chamber includes a sample application region and a protecting cover, and the sample application region is set to sample and acquires card
On, and it is located at the fringe region of sample acquisition card, the protecting cover is a flake structure, and one end of the flake structure is rotatablely connected
In a side of sample acquisition card, and the flake structure can be on the basis of the side, relative sample capture card
Piece is turned down to lid and is closed in the top of sample application region, and the flake structure is equipped with and pastes portion, this pastes portion can be by the thin slice
Structure is pasted on sample capture card, so that sampling filter paper is stored in the sample application region of filter paper preserving chamber；
A window to match with sample application region is offered on the protecting cover, when flake structure lid closes above sample application region,
The window is aligned with the sample application region；The protecting cover is equipped with to be pasted sealing strip and pastes envelope positioned at two sides positioned at upper end
Pig's tongue；
The intermediate region of the sampling filter paper sets there are two the position for limiting sample acquisition and definitely defines required sample
The circle of collection capacity, length direction parallel arrangement of two circles along sampling filter paper；The width of the sampling filter paper compares filter paper
The width of preserving chamber is 1-2 millimeters small；It is described sampling filter paper intermediate region set there are two for limit sample acquisition position and
The circle of required sample collection capacity is definitely defined, length direction parallel arrangement of two circles along sampling filter paper；The letter
Breath registration area is for registering by the information of sampler；
When using the anti-gyneduct hormone sampling kit sampling, operated according to the following steps:
Step 1 takes a sample acquisition card, conscientiously, clearly fills in all information of sample capture card on piece；
The protecting cover of sample acquisition card is turned down and covers conjunction on the sample application region by step 2；
The protecting cover is located at the pasting after sealing strip is attached to sample capture card on piece of upper end, so that protecting cover and sample-adding by step 3
The penetrating channel in a both ends is formed between region；
Step 4 will sample filter paper in the channel both ends insertion filter paper preserving chamber between protecting cover and sample application region；
Step 5 closes the channel both ends with the sealing strip of pasting of two sides, sampling filter paper can be fixed on filter paper preserving chamber
It is interior；
Step 6 at alcohol cotton stick wiping blood sampling, and waits alcohol to be completely dried；
Step 7 punctures skin at blood sampling with disposable blood taking needle；
Step 8 touches the sampling filter paper on paper jam after drop of blood is formed, and allows blood full of two circles, acquires blood volume in 40 μ l
Left and right；
Step 9, with the skin at the cleaning blood sampling of dry cotton swab；
Step 10, the sample capture card and desiccant that collect sample is sealed to be placed in valve bag together saves；
Collected sample carries out test sample preparation manipulation according to the following steps:
The first step is punched the blood cake for sampling filter paper using paper sheet punching machine；
The blood cake sample being drilled is put into centrifuge tube, and has marked bar code number by second step, which adopts with described
The bar code of bar code area is corresponding in sample kit；
Third step is added pre-configured sample buffer in the centrifuge tube for being packed into blood cake sample, and makes the sample
Buffer did not had the blood cake sample just；
The centrifuge tube that sample buffer is added in third step is placed in progress room temperature concussion in shaking table mechanism by the 4th step；
The centrifuge tube for passing through room temperature concussion in 4th step is placed in centrifugal mechanism and is centrifuged, makes blood cake sample complete by the 5th step
It is centrifuged to bottom；
6th step is put sample well, sequentially with use to be detected；
The sample buffer is configured using following steps:
Deionized water and trishydroxymethylaminomethane are configured to obtain 0.1mol/L by step S1 according to 1000: 12.1 mass ratio
Tris solution；
36~37% concentrated hydrochloric acid and water are mixed according to 9: 1000 volume ratio and shake up to obtain hydrochloric acid solution by step S2, closed
Storage；
Step S3, by hydrochloric acid solution obtained in tris solution obtained in step S1 and step S2 according to 50
: 44.7 volume ratio mixes；
The PH of mixed liquor in step S4, measuring process S3, and after adjusting the PH to 7.2 of the mixed liquor, mixed liquor is settled to
100ml obtains trishydroxymethylaminomethane-hydrochloric acid solution；
Step S5, according in below table with added in direction trishydroxymethylaminomethane-hydrochloric acid solution Tween-20 solution,
Fetal calf serum solution, NaCl solid and NaN3 solid；Wherein, the table are as follows:
。
2. anti-gyneduct hormone according to claim 1 samples kit, it is characterised in that: when sampling weather is cold
When, it puts his hand into warm bag or warm water 2-4 minutes.
3. anti-gyneduct hormone according to claim 1 samples kit, it is characterised in that: when sampling, with disposable
Blood taking needle punctures at blood sampling after skin, can be from referring to gently squeezing finger to finger tip.
4. anti-gyneduct hormone according to claim 1 samples kit, it is characterised in that: collected sample can be with
Pass through bar code or information preservation card approach management sample information.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610634942.4A CN106053860B (en) | 2016-07-29 | 2016-07-29 | A kind of anti-gyneduct hormone sampling kit and sampling and extracting method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610634942.4A CN106053860B (en) | 2016-07-29 | 2016-07-29 | A kind of anti-gyneduct hormone sampling kit and sampling and extracting method |
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| Publication Number | Publication Date |
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| CN106053860A CN106053860A (en) | 2016-10-26 |
| CN106053860B true CN106053860B (en) | 2018-12-28 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610634942.4A Active CN106053860B (en) | 2016-07-29 | 2016-07-29 | A kind of anti-gyneduct hormone sampling kit and sampling and extracting method |
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| Country | Link |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2022501398A (en) * | 2018-09-28 | 2022-01-06 | シーメンス・ヘルスケア・ダイアグノスティックス・インコーポレイテッド | Storage Solution Composition for Stabilization of Purified Muller Tube Suppressant (MIS) and Related Usage and Production Methods |
| CN113558619B (en) * | 2021-07-08 | 2023-06-16 | 重庆医药高等专科学校 | Automatic change blood sampling device |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102213649A (en) * | 2011-04-30 | 2011-10-12 | 杭州宝荣科技有限公司 | Acquisition card special for disease screening |
| CN203483432U (en) * | 2013-09-03 | 2014-03-19 | 广州达安临床检验中心有限公司 | Dried blood spot collecting device |
-
2016
- 2016-07-29 CN CN201610634942.4A patent/CN106053860B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102213649A (en) * | 2011-04-30 | 2011-10-12 | 杭州宝荣科技有限公司 | Acquisition card special for disease screening |
| CN203483432U (en) * | 2013-09-03 | 2014-03-19 | 广州达安临床检验中心有限公司 | Dried blood spot collecting device |
Non-Patent Citations (1)
| Title |
|---|
| McDade等.Quantification of anti-Mullerian hormone (AMH) in dried blood spots:validation of a minimally invasive method for assessing ovarian reserve.《human reproduction》.2012,第27卷(第8期),第2503-2508页. * |
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