CN106053848A - Kit for oculopathy detection and detection method - Google Patents

Kit for oculopathy detection and detection method Download PDF

Info

Publication number
CN106053848A
CN106053848A CN201610629367.9A CN201610629367A CN106053848A CN 106053848 A CN106053848 A CN 106053848A CN 201610629367 A CN201610629367 A CN 201610629367A CN 106053848 A CN106053848 A CN 106053848A
Authority
CN
China
Prior art keywords
sicam
aptamer
dna
seq
oculopathy
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
CN201610629367.9A
Other languages
Chinese (zh)
Inventor
李倩
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CN201610629367.9A priority Critical patent/CN106053848A/en
Publication of CN106053848A publication Critical patent/CN106053848A/en
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
    • G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70503—Immunoglobulin superfamily, e.g. VCAMs, PECAM, LFA-3
    • G01N2333/70525—ICAM molecules, e.g. CD50, CD54, CD102
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/04—Endocrine or metabolic disorders
    • G01N2800/046—Thyroid disorders
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/16—Ophthalmology

Abstract

The invention discloses an aptamer specially bonded with sICAM-1 and a kit. The provided aptamer has preferable affinity for sICAM-1 and can capture sICAM-1 in a solution. A kit prepared from the aptamer is used for screening oculopathy. The kit has characteristics of high sensitivity and low cost and is suitable for large-scale screening.

Description

A kind of test kit for detecting oculopathy and detection method thereof
Technical field
The present invention relates to a kind of test kit for detecting oculopathy and detection method thereof.
Background technology
Thyroid-associated ophthalmopathy (thyroid associated ophthalmopathy, TAO) is the common disease of department of endocrinology One of sick, also it is to treat one of disease the most difficult at present in the world.The TAO course of disease is longer, and each age group all can occur, with female Property is multiple.The lighter can affect life, work quality, damages appearance, and severe one may result in visual deterioration, the most blind, carries to patient Heavy economy and psychological burden are come.In recent years, due to living-pattern preservation, the increase of operating pressure, social environment shape The factors such as the change of condition, popularizing of edible iodized salt, and irregular medication, the sickness rate of TAO substantially rises, and admission rate is high. The brightest due to primary disease reason at present, clinical main with hormone, immunosuppressant, growth hormone analogs, antithyroid drug, socket of the eye The Comprehensive Treatments such as interior radiation, orbital decompression are main, but for various reasons, effect is the most unsatisfactory, there is no up to now and appoint TAO can effectively be treated by what a kind of medicine, and obtains long-term remission.Therefore, the medicine of research treatment thyroid-associated ophthalmopathy is urgently Solve the technical problem that.And detect the direction that described disease this area especially is actively studied.
Recent studies indicate that, the expression of intercellular adhesion molecule-1 and Autoimmune thyroid disease immunopathology Closely related.SICAM-1 and the sICAM-1 table of lymphocyte infiltration, endotheliocyte in the parathyroid tissue of thyroiditis patient Reach Showed Very Brisk.Additionally, it was found that increase with patients serum's sICAM-1 horizontal abnormality of expophthalmos, this is because after patient's eyeball Tissue strong expression sICAM-1, so the level that serum sICAM-1 raises is how closely related with socket of the eye week inflammatory infiltration degree.This Outward, research also finds, in the therapeutic process of medicine, and thyroid function and the improvement of clinical symptoms and Serum sICAM-1 Decline asynchronous, often the recovery of thyroid function to a great extent will be early than the recovery of sICAM-1.In sum, The concentration change of Soluble ICAM-1 is possibly for judging that the autoimmune disorder level of GD, drug withdrawal whether and are No recurrences etc. are significant.The expression of intercellular adhesion molecule-1 and thyroid-associated ophthalmopathy have direct correlation, therefore, and inspection Survey the expression of intercellular adhesion molecule-1, be used directly for identifying thyroid-associated ophthalmopathy.But traditional detection is thin The expression of ICAM-1 has operation complexity, time-consuming shortcoming.
Aptamer (Aptamer, also known as aptamers, aptamer) is can high-affinity, certain life of combination of high specific Thing leather El target strand widow's nucleic acid molecules (ssDNA or ssRNA).Aptamer is by index concentration Fas lignand system evolution technology (Systemat1c Evolut1on of L1gands by Exponent1al enr1chment, SELEX) is from synthetic What in DNA/RNA library, screening obtained can combine the single stranded DNA/RNA of target molecules by high degree of specificity.Report aptamer Target include metal ion, organic molecule, polypeptide, protein, cell even tissue etc..The molecular recognition merit of aptamer Can be similar with antibody, there is the target identification ability the most higher with antibody molecule, but have the most excellent compared with antibody Good characteristic, as molecular weight is little, can manufacture, not easy in inactivation, non-immunogenicity, be readily synthesized with labelling, quickly penetrate Between tissue, good dynamic metabolism, different batches, product does not haves difference and has fine chemical stability, at biology The fields such as detection, medical diagnosis on disease treatment have important application prospect.
Summary of the invention
It is an object of the invention to provide aptamer and the test kit thereof of a kind of specific bond sICAM-1.
The aptamer that the present invention provides, is the single stranded DNA shown in sequence 1-15 of sequence table.
Described aptamer and sICAM-1 albumen have preferable affinity.
Also described aptamer can be modified or transformed, obtain the derivant of described aptamer.
The derivant of described aptamer can be following any one:
A) described aptamer being deleted part or increases the nucleotide of partial complementarity, obtain has with described aptamer There is the derivant of the aptamer of identical function；
B) described aptamer carrying out nucleotide replacement or part is modified, obtain has identical with described aptamer The derivant of the aptamer of function；
C) transforming the skeleton of described aptamer as phosphorothioate backbone, obtain has phase with described aptamer The derivant of the aptamer of congenerous；
D) aptamer transform peptide nucleic acid(PNA) as, obtain has the aptamer of identical function with described aptamer Derivant；
E) after described aptamer being connected upper fluorescence, radioactivity and therapeutic substance, that obtain with described aptamer There is the derivant of the aptamer of identical function.
Described aptamer can be used for the test kit of preparation detection sICAM-1.
Utilize the aptamer of the present invention, the sICAM-1 in blood can be captured, thus sieve for thyroid-associated ophthalmopathy Look into.Utilize the aptamer of the present invention, part to replace monoclonal antibody capture sICAM-1 to detect, there is highly sensitive, cost Preparation low, easy, the advantage easily preserved.The present invention has the highest using value.
Detailed description of the invention
Below example facilitates a better understanding of the present invention, but does not limit the present invention.Experiment in following embodiment Method, if no special instructions, is conventional method.
Embodiment 1, the screening of aptamer and preparation
Two ends comprise about 20 nucleotide, centre includes that the random nucleic acid library of 39 nucleotide is as follows in design:
5’-ACGACTAACGTGTAACAG(N39)CAGTGCATGATGCTACGAA-3’；N39 represents 39 random nucleoside Acid.
Being double-stranded DNA by single-stranded DNA banks amplification, product is through 2% agarose gel electrophoresis and cuts glue recovery purification；To return The double-stranded DNA received is template, and in vitro transcription goes out single stranded RNA random library, and transcription product is through PAGE purification.75 μ g RNA library warps The anti-sieve of nitrocellulose filter removes the RNA molecule being combined with film, and then (by Chen Zhihong etc., people is thin with 2ug sICAM-1 albumen The eukaryotic cell expression of intercellular adhesion molecule-1 and the method disclosed in qualification, express the destination protein obtained), hatch for 37 DEG C 30min, reactant liquor filters through nitrocellulose filter, washs filter membrane；Then filter membrane is shredded, be placed in elution buffer (6mol/L Carbamide, 0.55mol/L ammonium acetate, l.5mmol/L EDTA, 0.15%SDS) in boil 5min, centrifugal, take supernatant, dehydrated alcohol Precipitation RNA, and be redissolved in 20 μ 1DEPC water；With RNA for template RT-PCR amplifying doulbe-chain DNA, in vitro transcription goes out RNA literary composition Storehouse is screened for next round；Often wheel screening process in RT-PCR obtain double-stranded DNA library, with this double-stranded DNA as template body outside turn Recording out RNA aptamer storehouse, screening carries out 10 altogether and takes turns.Having obtained 15 aptamers, its sequence is respectively SEQ ID NO:1-15 institute Show.Particular sequence is as follows:
SICAM-1-1:
ACGACTAACGTGTAACAGTCCAAGTATTCATACATACCATAAATTCATTGTCATTAACAGTGCATGATGCTACGAA (SEQ ID NO:1)
SICAM-1-2:
ACGACTAACGTGTAACAGCAGACACCATATATACTATCTACTACCACATACCTCTACCAGTGCATGATGCTACGAA (SEQ ID NO:2)
SICAM-1-3:
ACGACTAACGTGTAACAGCTAACTTCTTATAACTTAAGATACTTCTTCCAAACAACACAGTGCATGATGCTACGAA (SEQ ID NO:3)
SICAM-1-4:
ACGACTAACGTGTAACAGTACATCACTCTAATCTTCACGCCTCAGTTACATTCCCTTCAGTGCATGATGCTACGAA (SEQ ID NO:4)
SICAM-1-5:
ACGACTAACGTGTAACAGATGTATATTCCTTATATGACTATAATTCTTACCTATAAACAGTGCATGATGCTACGAA (SEQ ID NO:5)
SICAM-1-6:
ACGACTAACGTGTAACAGACCTCTATCTCACTCTTCCCTTTCGCCTACACATCCGAACAGTGCATGATGCTACGAA (SEQ ID NO:6)
SICAM-1-7:
ACGACTAACGTGTAACAGAGATTCACTAATATTTCTTCGCCTCGCCATACTTAAGATCAGTGCATGATGCTACGAA (SEQ ID NO:7)
SICAM-1-8:
ACGACTAACGTGTAACAGTACCCATCCACCTTCTTACTACTGCTCTCTCAATCTACACAGTGCATGATGCTACGAA (SEQ ID NO:8)
SICAM-1-9:
ACGACTAACGTGTAACAGACAGATACATTCATATCAAGTTACACATCTCCATGTTAACAGTGCATGATGCTACGAA (SEQ ID NO:9)
SICAM-1-10:
ACGACTAACGTGTAACAGCAGAACACAAATCTATACAAATCAGCATCACACTCATACCAGTGCATGATGCTACGAA (SEQ ID NO:10)
SICAM-1-11:
ACGACTAACGTGTAACAGATACCAATCGCCACCTACACTTACATCTAGACACTCTATCAGTGCATGATGCTACGAA (SEQ ID NO:11)
SICAM-1-12:
ACGACTAACGTGTAACAGTATCTACACATAGATCCTCCACTTAATAACAATCTGTCACAGTGCATGATGCTACGAA (SEQ ID NO:12)
SICAM-1-13:
ACGACTAACGTGTAACAGATACCTCATCAGATATTACATTCGCCAATATTTCTCTATCAGTGCATGATGCTACGAA (SEQ ID NO:13)
SICAM-1-14:
ACGACTAACGTGTAACAGTCTATAACGCCTAACTACACCACTCCACTCATTATAATACAGTGCATGATGCTACGAA (SEQ ID NO:14)
SICAM-1-15:
ACGACTAACGTGTAACAGATCTATTTCACCATTAAGAACCATATCGCATCACCCTTCCAGTGCATGATGCTACGAA (SEQ ID NO:15)
The performance measurement of embodiment 2 sICAM-1 protein binding aptamer
RNA aptamer taking 2.0 μ g respectively, digests lh with calf intestinal alkaline phosphatase (CIP) 37 DEG C, purification reclaims dephosphorization The RNA of acidifying；By T4 polynucleotide kinase labelling [γ-32P] ATP in dephosphorylized RNA molecule end.10nmol radiates Property labelling RNA aptamer sICAM-1 37 DEG C with variable concentrations (1-200nM) respectively hatch 30min, each group reactant liquor warp Nitrocellulose filter filters, and washs filter membrane, is dried filter membrane, and liquid scintillation counter measures the exit dose of residual, same sample on filter membrane Parallel do twice mensuration.Calculate the dissociation constant of each aptamer and sICAM-1.Result is as follows:
Title Dissociation constant Kd (unit nM)
sICAM-1-1 8.8
sICAM-1-2 8.9
sICAM-1-3 9.0
sICAM-1-4 7.9
sICAM-1-5 8.3
sICAM-1-6 8.2
sICAM-1-7 8.9
sICAM-1-8 9.3
sICAM-1-9 8.7
sICAM-1-10 8.1
sICAM-1-11 8.0
sICAM-1-12 9.4
sICAM-1-13 7.8
sICAM-1-14 8.6
sICAM-1-15 8.9
PBS blank Without binding ability
Aptamer specificity analyses and stability analysis described in embodiment 3
It is respectively adopted human albumin, immune globulin, vibrio cholera VgrG3C albumen, escherichia coli outer membrane protein A, Lp-PLA2 albumen, carries out specific detection with 15 aptamers, through binding tests find, these aptamers the most not with this A little albumen combine, and are only combined the specificity keeping higher with sICAM-1.
By described aptamer, take 0.2ug, be respectively placed in the serum of room temperature, aqueous solution, place two weeks.Pass through RT- PCR detects, and finds its Stability Analysis of Structures of placement of two weeks, is not degraded.
The diagnosis of aptamer disease described in embodiment 4
By 15 aptamers respectively with the blood mixing 30min of 10 Patients with Thyroid Associated Ophthalmopathies and normal person, logical Cross biotin to separate, the content of quantitative analysis sICAM-1 therein albumen, found by analysis, 10 thyroid-associated ophthalmopathies In patient, the content of sICAM-1 albumen dramatically increases relative to normal person.
These are only the preferred embodiments of the present invention, be not limited to the present invention, for those skilled in the art For Yuan, all any modification, equivalent substitution and improvement etc. done within the spirit and principles in the present invention, should be included in this Within the protection domain of invention.
Sequence table
＜ 110 ＞ Li Qian
＜ 120 ＞ mono-kind is for detecting test kit and the detection method thereof of oculopathy
〈160〉14
〈210〉1
〈211〉76
〈212〉DNA
＜ 213 ＞ artificial sequence
＜ 400 ＞ sICAM-1-1:
ACGACTAACGTGTAACAGTCCAAGTATTCATACATACCATAAATTCATTGTCATTAA CAGTGCATGATGCTACGAA
〈210〉2
〈211〉76
〈212〉DNA
＜ 213 ＞ artificial sequence
＜ 400 ＞ sICAM-1-2:
ACGACTAACGTGTAACAGCAGACACCATATATACTATCTACTACCACATACCTCTAC CAGTGCATGATGCTACGAA
〈210〉3
〈211〉76
〈212〉DNA
＜ 213 ＞ artificial sequence
＜ 400 ＞ sICAM-1-3:
ACGACTAACGTGTAACAGCTAACTTCTTATAACTTAAGATACTTCTTCCAAACAACA CAGTGCATGATGCTACGAA
〈210〉4
〈211〉76
〈212〉DNA
＜ 213 ＞ artificial sequence
＜ 400 ＞ sICAM-1-4:
ACGACTAACGTGTAACAGTACATCACTCTAATCTTCACGCCTCAGTTACATTCCCTT CAGTGCATGATGCTACGAA
〈210〉5
〈211〉76
〈212〉DNA
＜ 213 ＞ artificial sequence
＜ 400 ＞ sICAM-1-5:
ACGACTAACGTGTAACAGATGTATATTCCTTATATGACTATAATTCTTACCTATAAA CAGTGCATGATGCTACGAA
〈210〉6
〈211〉76
〈212〉DNA
＜ 213 ＞ artificial sequence
＜ 400 ＞ sICAM-1-6:
ACGACTAACGTGTAACAGACCTCTATCTCACTCTTCCCTTTCGCCTACACATCCGAA CAGTGCATGATGCTACGAA
〈210〉7
〈211〉76
〈212〉DNA
＜ 213 ＞ artificial sequence
＜ 400 ＞ sICAM-1-7:
ACGACTAACGTGTAACAGAGATTCACTAATATTTCTTCGCCTCGCCATACTTAAGAT CAGTGCATGATGCTACGAA
〈210〉8
〈211〉76
〈212〉DNA
＜ 213 ＞ artificial sequence
＜ 400 ＞ sICAM-1-8:
ACGACTAACGTGTAACAGTACCCATCCACCTTCTTACTACTGCTCTCTCAATCTACA CAGTGCATGATGCTACGAA
〈210〉9
〈211〉76
〈212〉DNA
＜ 213 ＞ artificial sequence
＜ 400 ＞ sICAM-1-9:
ACGACTAACGTGTAACAGACAGATACATTCATATCAAGTTACACATCTCCATGTTAA CAGTGCATGATGCTACGAA
〈210〉10
〈211〉76
〈212〉DNA
＜ 213 ＞ artificial sequence
＜ 400 ＞ sICAM-1-10:
ACGACTAACGTGTAACAGCAGAACACAAATCTATACAAATCAGCATCACACTCATAC CAGTGCATGATGCTACGAA
〈210〉11
〈211〉76
〈212〉DNA
＜ 213 ＞ artificial sequence
＜ 400 ＞ sICAM-1-11:
ACGACTAACGTGTAACAGATACCAATCGCCACCTACACTTACATCTAGACACTCTAT CAGTGCATGATGCTACGAA
〈210〉12
〈211〉76
〈212〉DNA
＜ 213 ＞ artificial sequence
＜ 400 ＞ sICAM-1-12:
ACGACTAACGTGTAACAGTATCTACACATAGATCCTCCACTTAATAACAATCTGTCA CAGTGCATGATGCTACGAA
〈210〉13
〈211〉76
〈212〉DNA
＜ 213 ＞ artificial sequence
＜ 400 ＞ sICAM-1-13:
ACGACTAACGTGTAACAGATACCTCATCAGATATTACATTCGCCAATATTTCTCTAT CAGTGCATGATGCTACGAA
〈210〉14
〈211〉76
〈212〉DNA
＜ 213 ＞ artificial sequence
＜ 400 ＞ sICAM-1-14:
ACGACTAACGTGTAACAGTCTATAACGCCTAACTACACCACTCCACTCATTATAATA CAGTGCATGATGCTACGAA
〈210〉15
〈211〉76
〈212〉DNA
＜ 213 ＞ artificial sequence
＜ 400 ＞ sICAM-1-15:
ACGACTAACGTGTAACAGATCTATTTCACCATTAAGAACCATATCGCATCACCCTTC CAGTGCATGATGCTACGAA

Claims (5)

1. the test kit for oculopathy detection, it is characterised in that: comprise aptamer.
2. test kit as claimed in claim 1, it is characterised in that: described aptamer is described in SEQ ID No:7.
3. the fit application in the test kit of preparation detection oculopathy described in SEQ ID No:7.
4. the test kit as described in claim 1-2, it is characterised in that: described oculopathy is thyroid-associated ophthalmopathy.
Apply the most as claimed in claim 3, it is characterised in that: described oculopathy is thyroid-associated ophthalmopathy.
CN201610629367.9A 2015-11-01 2015-11-01 Kit for oculopathy detection and detection method Withdrawn CN106053848A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610629367.9A CN106053848A (en) 2015-11-01 2015-11-01 Kit for oculopathy detection and detection method

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201610629367.9A CN106053848A (en) 2015-11-01 2015-11-01 Kit for oculopathy detection and detection method
CN201510723711.6A CN105334327B (en) 2015-11-01 2015-11-01 Kit and method for detecting ophthalmopathy

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN201510723711.6A Division CN105334327B (en) 2015-11-01 2015-11-01 Kit and method for detecting ophthalmopathy

Publications (1)

Publication Number Publication Date
CN106053848A true CN106053848A (en) 2016-10-26

Family

ID=55284978

Family Applications (15)

Application Number Title Priority Date Filing Date
CN201610629097.1A Withdrawn CN105974140A (en) 2015-11-01 2015-11-01 Kit for detecting eye diseases and detection method of kit
CN201610628671.1A Withdrawn CN106018834A (en) 2015-11-01 2015-11-01 Kit for detecting eye diseases and detection method thereof
CN201610628761.0A Withdrawn CN106199008A (en) 2015-11-01 2015-11-01 A kind of test kit for detecting oculopathy and detection method thereof
CN201610628596.9A Withdrawn CN105974138A (en) 2015-11-01 2015-11-01 Kit for detecting eye diseases and detection method of kit
CN201610628554.5A Withdrawn CN106248964A (en) 2015-11-01 2015-11-01 A kind of test kit for detecting oculopathy and detection method thereof
CN201610628672.6A Withdrawn CN105974139A (en) 2015-11-01 2015-11-01 Kit for detecting eye diseases and detection method of kit
CN201610628675.XA Withdrawn CN106018835A (en) 2015-11-01 2015-11-01 Kit for detecting eye diseases and detection method thereof
CN201610628827.6A Withdrawn CN106248965A (en) 2015-11-01 2015-11-01 A kind of test kit for detecting oculopathy and detection method thereof
CN201610629366.4A Withdrawn CN106199009A (en) 2015-11-01 2015-11-01 A kind of test kit for detecting oculopathy and detection method thereof
CN201610629215.9A Withdrawn CN106248966A (en) 2015-11-01 2015-11-01 A kind of test kit for detecting oculopathy and detection method thereof
CN201510723711.6A Active CN105334327B (en) 2015-11-01 2015-11-01 Kit and method for detecting ophthalmopathy
CN201610628509.XA Withdrawn CN106199006A (en) 2015-11-01 2015-11-01 A kind of test kit for detecting oculopathy and detection method thereof
CN201610628510.2A Withdrawn CN105974137A (en) 2015-11-01 2015-11-01 Kit for detecting eye diseases and detection method of kit
CN201610629367.9A Withdrawn CN106053848A (en) 2015-11-01 2015-11-01 Kit for oculopathy detection and detection method
CN201610628826.1A Withdrawn CN106018836A (en) 2015-11-01 2015-11-01 Kit for detecting eye diseases and detection method thereof

Family Applications Before (13)

Application Number Title Priority Date Filing Date
CN201610629097.1A Withdrawn CN105974140A (en) 2015-11-01 2015-11-01 Kit for detecting eye diseases and detection method of kit
CN201610628671.1A Withdrawn CN106018834A (en) 2015-11-01 2015-11-01 Kit for detecting eye diseases and detection method thereof
CN201610628761.0A Withdrawn CN106199008A (en) 2015-11-01 2015-11-01 A kind of test kit for detecting oculopathy and detection method thereof
CN201610628596.9A Withdrawn CN105974138A (en) 2015-11-01 2015-11-01 Kit for detecting eye diseases and detection method of kit
CN201610628554.5A Withdrawn CN106248964A (en) 2015-11-01 2015-11-01 A kind of test kit for detecting oculopathy and detection method thereof
CN201610628672.6A Withdrawn CN105974139A (en) 2015-11-01 2015-11-01 Kit for detecting eye diseases and detection method of kit
CN201610628675.XA Withdrawn CN106018835A (en) 2015-11-01 2015-11-01 Kit for detecting eye diseases and detection method thereof
CN201610628827.6A Withdrawn CN106248965A (en) 2015-11-01 2015-11-01 A kind of test kit for detecting oculopathy and detection method thereof
CN201610629366.4A Withdrawn CN106199009A (en) 2015-11-01 2015-11-01 A kind of test kit for detecting oculopathy and detection method thereof
CN201610629215.9A Withdrawn CN106248966A (en) 2015-11-01 2015-11-01 A kind of test kit for detecting oculopathy and detection method thereof
CN201510723711.6A Active CN105334327B (en) 2015-11-01 2015-11-01 Kit and method for detecting ophthalmopathy
CN201610628509.XA Withdrawn CN106199006A (en) 2015-11-01 2015-11-01 A kind of test kit for detecting oculopathy and detection method thereof
CN201610628510.2A Withdrawn CN105974137A (en) 2015-11-01 2015-11-01 Kit for detecting eye diseases and detection method of kit

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN201610628826.1A Withdrawn CN106018836A (en) 2015-11-01 2015-11-01 Kit for detecting eye diseases and detection method thereof

Country Status (1)

Country Link
CN (15) CN105974140A (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020020947A1 (en) * 2018-07-26 2020-01-30 Bracco Imaging Spa Icam-1 aptamers, diagnostic and therapeutic uses thereof

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101148667B (en) * 2006-09-22 2012-05-30 国家纳米技术与工程研究院 Preparation and use for affinity human albumin nucleic acid aptamer
CN101144814A (en) * 2007-10-22 2008-03-19 中国人民解放军第三军医大学第一附属医院 Method for detecting, identifying and/ or quantifying compound using adapter type reagent
CN104818278A (en) * 2015-04-17 2015-08-05 刘红卫 Aptamer capable of being in specific binding to TS/MDEP protein in gastric cancer cells
CN104830867A (en) * 2015-06-07 2015-08-12 杨洋 Aptamer capable of being specifically combined with DKK1 protein in cancer cells

Also Published As

Publication number Publication date
CN106199009A (en) 2016-12-07
CN105334327A (en) 2016-02-17
CN106018834A (en) 2016-10-12
CN106248964A (en) 2016-12-21
CN105974137A (en) 2016-09-28
CN105334327B (en) 2017-03-22
CN105974138A (en) 2016-09-28
CN106018835A (en) 2016-10-12
CN105974139A (en) 2016-09-28
CN106199006A (en) 2016-12-07
CN106248965A (en) 2016-12-21
CN106018836A (en) 2016-10-12
CN106248966A (en) 2016-12-21
CN106199008A (en) 2016-12-07
CN105974140A (en) 2016-09-28

Similar Documents

Publication Publication Date Title
CN106053848A (en) Kit for oculopathy detection and detection method
CN105203763B (en) A kind of test kit of oral cancer specific detection
CN105334328B (en) Kit and method for detecting ophthalmic diseases
CN105223358B (en) A kind of test kit of carcinoma of endometrium specific detection
CN105353126B (en) A kind of test kit of lip cancer specific detection
CN105177013A (en) Kit for detecting cerebrovascular diseases
CN105823895A (en) Kit for specific detection of endometrial cancer
CN105823879A (en) Kit for specific detection of endometrial cancer
CN105823894A (en) Kit for specific detection of endometrial cancer

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
WW01 Invention patent application withdrawn after publication
WW01 Invention patent application withdrawn after publication

Application publication date: 20161026