CN106045197B - The method for handling pesticide residue and heavy metal Hg in sewage - Google Patents
The method for handling pesticide residue and heavy metal Hg in sewage Download PDFInfo
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- CN106045197B CN106045197B CN201610436494.7A CN201610436494A CN106045197B CN 106045197 B CN106045197 B CN 106045197B CN 201610436494 A CN201610436494 A CN 201610436494A CN 106045197 B CN106045197 B CN 106045197B
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- 239000010865 sewage Substances 0.000 title claims abstract description 38
- 238000000034 method Methods 0.000 title claims abstract description 32
- 239000000447 pesticide residue Substances 0.000 title claims abstract description 17
- 229910001385 heavy metal Inorganic materials 0.000 title claims description 12
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 claims abstract description 18
- 229910052753 mercury Inorganic materials 0.000 claims abstract description 18
- 108090000623 proteins and genes Proteins 0.000 claims description 18
- 241000589776 Pseudomonas putida Species 0.000 claims description 11
- 238000012545 processing Methods 0.000 claims description 11
- 230000008569 process Effects 0.000 claims description 9
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 8
- 102000004169 proteins and genes Human genes 0.000 claims description 8
- 230000001580 bacterial effect Effects 0.000 claims description 7
- 108090000854 Oxidoreductases Proteins 0.000 claims description 6
- 102000004316 Oxidoreductases Human genes 0.000 claims description 6
- 108010045510 NADPH-Ferrihemoprotein Reductase Proteins 0.000 claims description 5
- 102100023897 NADPH-cytochrome P450 reductase Human genes 0.000 claims description 5
- 229910021536 Zeolite Inorganic materials 0.000 claims description 4
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 4
- 230000001376 precipitating effect Effects 0.000 claims description 4
- 238000001179 sorption measurement Methods 0.000 claims description 4
- 239000010457 zeolite Substances 0.000 claims description 4
- 150000001413 amino acids Chemical class 0.000 claims description 3
- 230000003698 anagen phase Effects 0.000 claims description 3
- 230000035772 mutation Effects 0.000 claims description 3
- 102000002004 Cytochrome P-450 Enzyme System Human genes 0.000 claims 2
- 108010015742 Cytochrome P-450 Enzyme System Proteins 0.000 claims 2
- 108090000790 Enzymes Proteins 0.000 claims 1
- 239000000575 pesticide Substances 0.000 abstract description 16
- 230000008901 benefit Effects 0.000 abstract description 3
- 238000011109 contamination Methods 0.000 abstract 1
- 235000016709 nutrition Nutrition 0.000 abstract 1
- 239000002689 soil Substances 0.000 description 9
- 239000000126 substance Substances 0.000 description 7
- 230000000694 effects Effects 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 241000607479 Yersinia pestis Species 0.000 description 4
- 101150067427 por gene Proteins 0.000 description 4
- 239000005916 Methomyl Substances 0.000 description 3
- 230000015556 catabolic process Effects 0.000 description 3
- 238000006731 degradation reaction Methods 0.000 description 3
- 238000003912 environmental pollution Methods 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- UHXUZOCRWCRNSJ-QPJJXVBHSA-N methomyl Chemical compound CNC(=O)O\N=C(/C)SC UHXUZOCRWCRNSJ-QPJJXVBHSA-N 0.000 description 3
- 238000003672 processing method Methods 0.000 description 3
- 238000003900 soil pollution Methods 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 102220576918 C2 calcium-dependent domain-containing protein 4B_D346E_mutation Human genes 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 102220469950 Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN_D137A_mutation Human genes 0.000 description 2
- 206010035148 Plague Diseases 0.000 description 2
- 241000700159 Rattus Species 0.000 description 2
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 2
- 238000009933 burial Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- MCWXGJITAZMZEV-UHFFFAOYSA-N dimethoate Chemical compound CNC(=O)CSP(=S)(OC)OC MCWXGJITAZMZEV-UHFFFAOYSA-N 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 239000003344 environmental pollutant Substances 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 231100000719 pollutant Toxicity 0.000 description 2
- 102220093732 rs876660742 Human genes 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 239000002351 wastewater Substances 0.000 description 2
- 229910052725 zinc Inorganic materials 0.000 description 2
- 239000011701 zinc Substances 0.000 description 2
- 206010008428 Chemical poisoning Diseases 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 102000018832 Cytochromes Human genes 0.000 description 1
- 108010052832 Cytochromes Proteins 0.000 description 1
- 206010064571 Gene mutation Diseases 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 101150053185 P450 gene Proteins 0.000 description 1
- 239000004411 aluminium Substances 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- UYJXRRSPUVSSMN-UHFFFAOYSA-P ammonium sulfide Chemical compound [NH4+].[NH4+].[S-2] UYJXRRSPUVSSMN-UHFFFAOYSA-P 0.000 description 1
- 239000012223 aqueous fraction Substances 0.000 description 1
- 229910052785 arsenic Inorganic materials 0.000 description 1
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 description 1
- 150000007516 brønsted-lowry acids Chemical class 0.000 description 1
- 150000007528 brønsted-lowry bases Chemical class 0.000 description 1
- 229910052793 cadmium Inorganic materials 0.000 description 1
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 231100000749 chronicity Toxicity 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 238000005034 decoration Methods 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000001784 detoxification Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000004134 energy conservation Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 238000005189 flocculation Methods 0.000 description 1
- 230000016615 flocculation Effects 0.000 description 1
- 238000010413 gardening Methods 0.000 description 1
- 238000012239 gene modification Methods 0.000 description 1
- 230000005017 genetic modification Effects 0.000 description 1
- 235000013617 genetically modified food Nutrition 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000007403 mPCR Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000000869 mutational effect Effects 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 230000002688 persistence Effects 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 238000012805 post-processing Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000036301 sexual development Effects 0.000 description 1
- 238000004162 soil erosion Methods 0.000 description 1
- 239000002352 surface water Substances 0.000 description 1
- 239000002341 toxic gas Substances 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 238000003911 water pollution Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F9/00—Multistage treatment of water, waste water or sewage
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
- C12N9/0038—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
- C12N9/0042—NADPH-cytochrome P450 reductase (1.6.2.4)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y106/00—Oxidoreductases acting on NADH or NADPH (1.6)
- C12Y106/02—Oxidoreductases acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
- C12Y106/02004—NADPH-hemoprotein reductase (1.6.2.4), i.e. NADP-cytochrome P450-reductase
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/28—Treatment of water, waste water, or sewage by sorption
- C02F1/281—Treatment of water, waste water, or sewage by sorption using inorganic sorbents
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/28—Treatment of water, waste water, or sewage by sorption
- C02F1/283—Treatment of water, waste water, or sewage by sorption using coal, charred products, or inorganic mixtures containing them
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/50—Treatment of water, waste water, or sewage by addition or application of a germicide or by oligodynamic treatment
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
- C02F1/5236—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities using inorganic agents
- C02F1/5245—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities using inorganic agents using basic salts, e.g. of aluminium and iron
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
- C02F1/54—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities using organic material
- C02F1/56—Macromolecular compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F2001/007—Processes including a sedimentation step
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/306—Pesticides
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2301/00—General aspects of water treatment
- C02F2301/08—Multistage treatments, e.g. repetition of the same process step under different conditions
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/04—Disinfection
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
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- Life Sciences & Earth Sciences (AREA)
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- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
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- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
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- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
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- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Processing Of Solid Wastes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Water Treatment By Sorption (AREA)
Abstract
It the present invention relates to the use of sewage treatment field, specifically a kind of method for reducing the pollution of mercury and pesticide residue in sewage.This method, which can effectively remove mercury pollution in sewage, has many advantages, such as that high-efficient, nutritional requirement is low, easy to operate and highly-safe, compared with other existing techniques, significantly reduce the cost that sewage mercury and pesticide residual contamination are administered, the efficiency that mercury and pollution by pesticides are administered in sewage is improved, there is very important practical application value.
Description
The application be the applying date be on June 22nd, 2015, application No. is 201510343959.X, it is entitled " processing
The divisional application of the application for a patent for invention of the method for pesticide residue and heavy metal Hg in sewage ".
Technical field
The invention belongs to sewage treatment fields, and in particular to the processing of pesticide residue and heavy metal Hg in sewage belongs to dirt
Water comprehensive treatment technique field.
Background technique
In recent decades, chemical pesticide is always the main means kind and pin that Pest organism, the plague of rats are controlled in the agricultural of gardens
Amount increases year by year.Chemical pesticide also destroys ecology, polluted environment, often while controlling Pest organism, the plague of rats improves yield
The 70% of the pesticide dosage of year more than 500,000 tons will flow into rivers soil, and the pesticide degradation speed having is slow, and pesticide is dense in air
Degree will maintain a considerable time, and due to its residual to the pollution in waters, food directly compromise human health and
The surface water of the safety of animal, pollution directly pollutes underground water again, and " mankind are just as great tutor Engels introduction
Subject the Nature revenge to be administered ".And be ensure grain have a good harvest, pesticide be again it is essential, therefore, detoxification, only
Change water resource, comprehensive treatment water environment pollution is very urgent, imperative.
Pollution by pesticides also results in high risks to human health.It is estimated that there are two million people's pesticide poisonings in the whole world every year,
20000 people are dead.Pimentel etc. thinks that the use of pesticide, every year up to 3,000,000,000 dollars, causes society to direct losses caused by the U.S.
8,100,000,000 dollars can be up to the total cost of environment, be equivalent to the 50% of 16,200,000,000 dollars of american chemical pesticide benefit value.So finding
The method of pollution by pesticides becomes important subject in processing environment.
In addition, environmental pollution also constantly aggravates with the continuous quickening of China's process of industrialization, administers the mankind and depend on for existence
Soil and atmosphere pollution it is extremely urgent.Other than the discharge of strict control pollutant, researchs and develops and be suitble to China's national situation
Environmental pollution treatment technology have great importance.Environmental pollution includes heavy metal pollution, pesticide and persistence organic compound
Object pollution, chemical fertilizer application pollution etc..With population increase and economic development, a large amount of untreated wastes are shifted to farmland,
The soil and water pollution problem that China faces are increasingly severe.The soil pollution in China in recent years is to the area of different scale
Domain sexual development, and the generation of various quality of agricultural product is seriously affected.According to incompletely statistics, the soil erosion of China's heavy metal pollution
Up to 20,000,000 hectares, the 1/6 of total area under cultivation is accounted for.Because of nearly 7,000,000 hectares of the farmland of Industrial " three Waste " pollution, subtract grain every year
Produce 10,000,000,000 kilograms.The half or so of the pollution gross area is accounted in the exceeded area of the elements such as some regional mercury, arsenic, copper, zinc.It is some
Main vegetables base Cadmium Pollution in Soils is universal, wherein zinc content in soil is up in some suburbs main facilities vegetable gardening fields
517 mg/kgs, as many as exceeded 5 times.Since soil pollution has concealment, latency and chronicity, serious consequence is only capable of
It is caused damages by food to animals and humans health, thus is not easy to be discovered by people.Heavy metal especially in soil has
Mobility is small, and the pollution time is long, by being either physically or chemically difficult the features such as thoroughly removing.It improves the ecological environment, protection soil
Loamy texture amount, control are polluted with rehabilitating soil, are just able to achieve agricultural safety, this is that face at present one of China is severe and urgent
Significant problem.
Currently, there are many pollution by pesticides and the processing method of heavy metal pollution, sum up including chemical treatment, burning,
The methods of burial and bioremediation.Chemical treatment method feasibility is strong, but generates a large amount of bronsted lowry acids and bases bronsted lowry during processing, these
Substance must also add processing again.Burning may be the degradation most reliable method of these pollutants.But it is released in treatment process big
The poison gas of amount, and need a large amount of thermal energy.There are also problems for burial, is mainly manifested in and causes to surrounding soil and water source
Secondary pollution.Therefore, it is extremely urgent to develop a kind of processing method that energy conservation and environmental protection is at low cost.
Summary of the invention
One of the objects of the present invention is to provide a kind of methods of pesticide residue and heavy metal Hg in processing sewage.
The present invention is achieved by the following technical scheme:
The method of pesticide residue and heavy metal Hg, includes the following steps: in a kind of processing sewage
(1) it pre-processes: by filtering and precipitating, removing the suspended matter in handled sewage；
(2) main process task: medicament ammonium sulfide, flocculation aid (polyacrylamide) and wadding is added according to mercury concentration in water in foul water fraction
Solidifying agent (aluminium polychloride), is added in sewage, and under normal temperature condition, flowing culture 24 hours can remove mercury in sewage；
(3) it post-processes: sewage being again passed by into zeolite and activated carbon adsorption tank, adsorbs pesticide residue；
(4) will treated sewage it is sterile-processed after, direct emission or reuse.
Another technical solution provided by the invention is:
The method of pesticide residue and heavy metal Hg, includes the following steps: in a kind of processing sewage
(1) it pre-processes: by filtering and precipitating, removing the suspended matter in handled sewage；
(2) main process task: the bacterium pseudomonas putida (Pseudomonas putida) of culture to logarithmic growth phase adds
Enter into sewage, under normal temperature condition, flowing culture 24 hours can remove mercury in sewage；
(3) it post-processes: sewage being again passed by into zeolite and activated carbon adsorption tank, adsorbs pesticide residue；
(4) will treated sewage it is sterile-processed after, direct emission or reuse.
Preferably, the pseudomonas putida is to be transferred to chtochrome oxidase reductase (P450CYP9G2) He Jingxiu
The Pseudomonas putida SPl CGMCC N0.3887 of the gene of the NADPH- cytochrome P450 reductase (POR) of decorations
Bacterial strain, the protein sequence of described chtochrome oxidase reductase (P450CYP9G2) coded by said gene is referring to GenBank:
The protein sequence of AB096739, the coded by said gene of the modified NADPH- cytochrome P450 reductase (POR) is
It carries out being mutated Asp44Ser (table as follows respectively relative to NADPH- cytochrome P450 reductase (POR) original amino acid
Show that the 44th Asp sports Ser), Gln74Tyr, Leu96Lys, Asp137Ala, His199Gly, Ile231Gly,
Asp296Glu, Asp346Glu, Pro381Cys, Tyr484Lys, Ser519Arg, Gln614His, NADPH- cytochromes
The original protein sequences of P450 reductase (POR) coded by said gene are referring to GenBank:L19897.
A series of bacterial strains by genetic modification provided by the invention not only have the effect of preferably going the removal of mercury, go back simultaneously
The pesticide residue in sewage can be effectively removed, with good application prospect.
The above method has the advantages that
1, processing method through the invention substantially increases the treatment effect of mercury and pesticide residue, when reducing reaction
Between.
2, the conversion bacterial strain of a kind of degrade mercury and pesticide residue, removal efficiency with higher are provided.
In order to make it easy to understand, the present invention will be described in detail by specific embodiment below.It needs to refer in particular to
Out, specific example is merely to illustrate, and be not meant to limit the scope of the invention.The ordinary skill people of obvious this field
Member can make various modifications and variations to the present invention within the scope of the invention according to illustrating herein, these amendments
It is also included in the scope of the present invention with changing.
Specific embodiment
The processing of embodiment 1 mercury and pesticide residue sewage
The concentration of mercury is 30mg/L, Rogor 20mg/L, fenifrothion 20mg/L, Methomyl 20mg/L when wastewater influent.
Pretreatment: by filtering and precipitating, the suspended matter in handled sewage is removed；
Main process task: by bacterium pseudomonas putida disclosed in CN 102373161A of the culture to logarithmic growth phase
Pseudomonas putida SPl CGMCC N0.3887, is added in sewage, and under normal temperature condition, flowing culture 24 is small
When, mercury in sewage can be removed；
Post-processing: again passing by zeolite and activated carbon adsorption tank for sewage and handle overnight, adsorbs pesticide residue；
Will treated sewage it is sterile-processed after, direct emission or reuse.Through detecting, the concentration of mercury is 2.2mg/L, happy
Fruit 15.4mg/L, fenifrothion 14.3mg/L, Methomyl 16.4mg/L.
The transformation and acquisition of 2 degrading pesticide gene of embodiment
According to method disclosed in CN1970741A, clone obtains CYP9G2 and POR gene.It is carried out for POR gene
Following transformation, by multiplex PCR by corresponding Asp44Ser (indicating that the 44th Asp sports Ser), Gln74Tyr,
Leu96Lys, Asp137Ala, His199Gly, Ile231Gly, Asp296Glu, Gly316Asn, Asp346Glu,
Pro381Cys, Tyr484Lys, Ser519Arg, Gln614His, mutational site are introduced between shown in GenBank:L19897
In albumen, to construct different mutant POR genes (preparation method that can refer to the prior art can be obtained).It will mutation
POR gene afterwards is connected according to the method for preparing CN1970741A respectively at expression vector together with CYP9G2 gene, will verify
Successful recombinant plasmid transformed enters in Pseudomonas putida SPl CGMCC N0.3887 bacterial strain, is detected by PCR,
It is constructed successfully.
The verifying of 3 sewage degradation effect of embodiment
The bacterial strain of different mutains will be contained, respectively all in accordance with the method for embodiment 1, verifies the processing to sewage and imitate
Fruit.The concentration of mercury is 30mg/L, Rogor 20mg/L, fenifrothion 20mg/L, Methomyl 20mg/L when wastewater influent.To be transferred to not
The bacterium of the bacterial strain sum of CYP9G2 and POR gene is mutated as control.Concrete outcome is as follows:
The bacterium that can be seen that POR the and CYP9G2 gene mutation for having imported specific mutation from above result has enhancing
The effect for removing the removal of mercury and pesticide, this absolutely prove is not arbitrary change all have preferable effect.Applicant passes through big
The verifying of amount just obtains several sites with better effects.
Claims (2)
1. the method for pesticide residue and heavy metal Hg, includes the following steps: in a kind of processing sewage
(1) it pre-processes: by filtering and precipitating, removing the suspended matter in handled sewage；
(2) main process task: the bacterium pseudomonas putida (Pseudomonas putida) of culture to logarithmic growth phase is added to
In sewage, under normal temperature condition, flowing culture 24 hours can remove mercury in sewage；
(3) it post-processes: sewage being again passed by into zeolite and activated carbon adsorption tank, adsorbs pesticide residue；
(4) will treated sewage it is sterile-processed after, direct emission or reuse；
The pseudomonas putida is to be transferred to chtochrome oxidase reductase and modified NADPH- Cytochrome P450
The Pseudomonas putida SPl CGMCC N0.3887 bacterial strain of the gene of reductase, the chtochrome oxidase reduction
The encoded protein sequence of enzyme gene is referring to GenBank:AB096739, and the modified NADPH- Cytochrome P450 is also
The protein sequence of the coded by said gene of protoenzyme is to carry out relative to NADPH- cytochrome P450 reductase original amino acid
Following mutation Ile231Gly, the original protein sequences of NADPH- cytochrome P450 reductase coded by said gene referring to
GenBank:L19897.
2. a kind of protein variant, it is characterised in that: the variant sequence thereof is as follows relative to the progress of albumen original amino acid
It is mutated Ile231Gly, albumen original protein sequences are referring to GenBank:L19897.
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| CN201610436464.6A Active CN105923912B (en) | 2015-06-22 | 2015-06-22 | The method for handling pesticide residue and heavy metal Hg in sewage |
| CN201610438372.1A Active CN105836981B (en) | 2015-06-22 | 2015-06-22 | The method of pesticide residue and heavy metal Hg in treated sewage |
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| CN201610438696.5A Active CN105836933B (en) | 2015-06-22 | 2015-06-22 | The method for handling pesticide residue and heavy metal Hg in sewage |
| CN201610438700.8A Active CN105836982B (en) | 2015-06-22 | 2015-06-22 | The method of pesticide residue and heavy metal Hg in treated sewage |
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| CN1970741A (en) * | 2005-11-23 | 2007-05-30 | 中国科学院动物研究所 | Super engineering bacteria, expressed detoxification enzyme thereof, and construction method and application therefor |
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| CN103771556B (en) * | 2014-01-13 | 2015-06-10 | 陕西师范大学 | Gas field mercury-containing sewage treatment method |
| CN104786370A (en) * | 2014-01-16 | 2015-07-22 | 王朝亮 | Concrete mixing truck for mine |
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Also Published As
| Publication number | Publication date |
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| CN105923837B (en) | 2019-06-11 |
| CN105836982A (en) | 2016-08-10 |
| CN105923914B (en) | 2018-10-09 |
| CN105836982B (en) | 2018-10-30 |
| CN106045197A (en) | 2016-10-26 |
| CN105923912B (en) | 2019-05-07 |
| CN105836983A (en) | 2016-08-10 |
| CN105836980B (en) | 2019-05-10 |
| CN105836933A (en) | 2016-08-10 |
| CN105836981B (en) | 2018-10-30 |
| CN104876371A (en) | 2015-09-02 |
| CN105836983B (en) | 2018-10-16 |
| CN105923917B (en) | 2018-11-02 |
| CN105923914A (en) | 2016-09-07 |
| CN105923912A (en) | 2016-09-07 |
| CN104876371B (en) | 2017-01-04 |
| CN105923916A (en) | 2016-09-07 |
| CN105836981A (en) | 2016-08-10 |
| CN105923917A (en) | 2016-09-07 |
| CN105923916B (en) | 2018-11-02 |
| CN105923837A (en) | 2016-09-07 |
| CN105836933B (en) | 2018-12-21 |
| CN105836980A (en) | 2016-08-10 |
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