CN105836982A - Method for treating pesticide residues and heavy metal mercury in sewage - Google Patents
Method for treating pesticide residues and heavy metal mercury in sewage Download PDFInfo
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- CN105836982A CN105836982A CN201610438700.8A CN201610438700A CN105836982A CN 105836982 A CN105836982 A CN 105836982A CN 201610438700 A CN201610438700 A CN 201610438700A CN 105836982 A CN105836982 A CN 105836982A
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- sewage
- pesticide residues
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- 239000010865 sewage Substances 0.000 title claims abstract description 43
- 238000000034 method Methods 0.000 title claims abstract description 39
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 title claims abstract description 21
- 239000000447 pesticide residue Substances 0.000 title claims abstract description 20
- 229910001385 heavy metal Inorganic materials 0.000 title claims description 12
- 229910052753 mercury Inorganic materials 0.000 title abstract description 7
- 230000008569 process Effects 0.000 claims abstract description 17
- 108090000623 proteins and genes Proteins 0.000 claims description 18
- 102100023897 NADPH-cytochrome P450 reductase Human genes 0.000 claims description 15
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 10
- 238000012545 processing Methods 0.000 claims description 10
- 241000589776 Pseudomonas putida Species 0.000 claims description 9
- 108010045510 NADPH-Ferrihemoprotein Reductase Proteins 0.000 claims description 8
- 230000008859 change Effects 0.000 claims description 8
- 102000004169 proteins and genes Human genes 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 230000001580 bacterial effect Effects 0.000 claims description 7
- 229910021536 Zeolite Inorganic materials 0.000 claims description 5
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 5
- 238000012805 post-processing Methods 0.000 claims description 5
- 238000001556 precipitation Methods 0.000 claims description 5
- 238000001179 sorption measurement Methods 0.000 claims description 5
- 239000010457 zeolite Substances 0.000 claims description 5
- 108090000854 Oxidoreductases Proteins 0.000 claims description 4
- 102000004316 Oxidoreductases Human genes 0.000 claims description 4
- 102220469950 Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN_D137A_mutation Human genes 0.000 claims description 4
- 150000001413 amino acids Chemical class 0.000 claims description 3
- 230000000844 anti-bacterial effect Effects 0.000 claims description 3
- 239000004411 aluminium Substances 0.000 claims description 2
- 229910052782 aluminium Inorganic materials 0.000 claims description 2
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims description 2
- UYJXRRSPUVSSMN-UHFFFAOYSA-P ammonium sulfide Chemical compound [NH4+].[NH4+].[S-2] UYJXRRSPUVSSMN-UHFFFAOYSA-P 0.000 claims description 2
- 239000012223 aqueous fraction Substances 0.000 claims description 2
- 239000003814 drug Substances 0.000 claims description 2
- 229920002401 polyacrylamide Polymers 0.000 claims 1
- 239000000575 pesticide Substances 0.000 abstract description 16
- 230000008901 benefit Effects 0.000 abstract description 3
- 235000016709 nutrition Nutrition 0.000 abstract 1
- 239000002689 soil Substances 0.000 description 9
- 230000000694 effects Effects 0.000 description 7
- 239000000126 substance Substances 0.000 description 7
- 241000607479 Yersinia pestis Species 0.000 description 4
- 101150067427 por gene Proteins 0.000 description 4
- MCWXGJITAZMZEV-UHFFFAOYSA-N dimethoate Chemical compound CNC(=O)CSP(=S)(OC)OC MCWXGJITAZMZEV-UHFFFAOYSA-N 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000003912 environmental pollution Methods 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 238000003672 processing method Methods 0.000 description 3
- 238000003900 soil pollution Methods 0.000 description 3
- 239000002351 wastewater Substances 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 102220576918 C2 calcium-dependent domain-containing protein 4B_D346E_mutation Human genes 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 239000005916 Methomyl Substances 0.000 description 2
- 101150053185 P450 gene Proteins 0.000 description 2
- 206010035148 Plague Diseases 0.000 description 2
- 241000589516 Pseudomonas Species 0.000 description 2
- 241000700159 Rattus Species 0.000 description 2
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 239000003344 environmental pollutant Substances 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- UHXUZOCRWCRNSJ-QPJJXVBHSA-N methomyl Chemical compound CNC(=O)O\N=C(/C)SC UHXUZOCRWCRNSJ-QPJJXVBHSA-N 0.000 description 2
- 231100000719 pollutant Toxicity 0.000 description 2
- 102220093732 rs876660742 Human genes 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- 229910052725 zinc Inorganic materials 0.000 description 2
- 239000011701 zinc Substances 0.000 description 2
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 206010064571 Gene mutation Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 229910052785 arsenic Inorganic materials 0.000 description 1
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 description 1
- 150000007516 brønsted-lowry acids Chemical class 0.000 description 1
- 150000007528 brønsted-lowry bases Chemical class 0.000 description 1
- 229910052793 cadmium Inorganic materials 0.000 description 1
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 231100000749 chronicity Toxicity 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000001784 detoxification Methods 0.000 description 1
- 238000004134 energy conservation Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 238000005189 flocculation Methods 0.000 description 1
- 230000016615 flocculation Effects 0.000 description 1
- 238000010413 gardening Methods 0.000 description 1
- 238000012239 gene modification Methods 0.000 description 1
- 230000005017 genetic modification Effects 0.000 description 1
- 235000013617 genetically modified food Nutrition 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000007403 mPCR Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000000869 mutational effect Effects 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000004162 soil erosion Methods 0.000 description 1
- 239000002352 surface water Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 238000003911 water pollution Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F9/00—Multistage treatment of water, waste water or sewage
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
- C12N9/0038—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
- C12N9/0042—NADPH-cytochrome P450 reductase (1.6.2.4)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y106/00—Oxidoreductases acting on NADH or NADPH (1.6)
- C12Y106/02—Oxidoreductases acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
- C12Y106/02004—NADPH-hemoprotein reductase (1.6.2.4), i.e. NADP-cytochrome P450-reductase
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/28—Treatment of water, waste water, or sewage by sorption
- C02F1/281—Treatment of water, waste water, or sewage by sorption using inorganic sorbents
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/28—Treatment of water, waste water, or sewage by sorption
- C02F1/283—Treatment of water, waste water, or sewage by sorption using coal, charred products, or inorganic mixtures containing them
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/50—Treatment of water, waste water, or sewage by addition or application of a germicide or by oligodynamic treatment
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
- C02F1/5236—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities using inorganic agents
- C02F1/5245—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities using inorganic agents using basic salts, e.g. of aluminium and iron
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
- C02F1/54—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities using organic material
- C02F1/56—Macromolecular compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F2001/007—Processes including a sedimentation step
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/306—Pesticides
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2301/00—General aspects of water treatment
- C02F2301/08—Multistage treatments, e.g. repetition of the same process step under different conditions
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/04—Disinfection
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Environmental & Geological Engineering (AREA)
- Hydrology & Water Resources (AREA)
- Water Supply & Treatment (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Processing Of Solid Wastes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Water Treatment By Sorption (AREA)
Abstract
The invention relates to the field of sewage treatment, in particular to a method for reducing pollution of mercury and pesticide residues in sewage. The method has the advantages of effective removal of pollution of mercury in sewage, high efficiency, low nutritional requirements, simple operation, high safety and the like. Compared with other processes, the cost of pollution abatement of mercury and pesticide residues in sewage is obviously reduced, the efficiency of pollution abatement of mercury and pesticides in sewage is improved, and the practical application value is very important.
Description
The application is filing date June 22, Application No. 201510343959.X, invention name in 2015
It is referred to as the divisional application of the application for a patent for invention of " processing sewage Pesticide Residues and the method for heavy metal Hg ".
Technical field
The invention belongs to sewage treatment area, be specifically related to the process of sewage Pesticide Residues and heavy metal Hg, belong to
In integrated wastewater processing technology field.
Background technology
In recent decades, Pest organism, the Main Means kind of the plague of rats are controlled during chemical pesticide is always gardens agricultural
Increase year by year with sales volume.Chemical pesticide, while controlling Pest organism, plague of rats raising yield, also destroys life
State, pollutes environment, and the 70% of the pesticide dosage of annual more than 500,000 tons is incited somebody to action and flowed into rivers soil, and have
Pesticide degradation speed is slow, and air Pesticides concentration will maintain a considerable time, and owing to its residual is to water
Territory, the pollution of food directly compromise the safety of health and animal, and the surface water of pollution the most directly pollutes
Subsoil water, as great tutor Engels teaching, " mankind are subject the report that the Nature is given
Multiple ".And for guaranteeing that grain is had a good harvest, pesticide is again requisite, therefore, detoxification, purification water resource,
Comprehensive control water environment pollution is very urgent, imperative.
Pollution by pesticides also results in high risks to human health.According to estimates, the whole world has two million people's pesticide every year
Poisoning, 20,000 people are dead.Pimentel etc. think that the direct losses caused to the U.S. that use of pesticide reach every year
3000000000 dollars, cause society to be up to 8,100,000,000 dollars with total cost of environment, be equivalent to american chemical pesticide benefit
It is worth the 50% of 16,200,000,000 dollars.So the method finding the pollution of processing environment Pesticides becomes important subject.
It addition, along with the continuous quickening of China's process of industrialization, environmental pollution the most constantly aggravates, administer the mankind and rely
Soil and atmospheric pollution with existence are extremely the most urgent.In addition to the strict discharge controlling pollutant, research is opened
The environmental pollution treatment technology sending out China's national situation applicable has great importance.Environmental pollution includes heavy metal-polluted
Dye, pesticide and persistency orgnic compound pollution, chemical fertilizer application pollution etc..Along with population increases and economic development,
A large amount of undressed garbages shift to farmland, and soil and water pollution problem that China faces are more and more tighter
Weight.The soil pollution of China in recent years is to the regional development of different scale, and to various quality of agricultural product
Generation has a strong impact on.According to incompletely statistics, the soil erosion of China's heavy metal pollution reaches 20,000,000 hectares, accounts for
The 1/6 of total area under cultivation.Nearly 7,000,000 hectares of the farmland polluted because of Industrial " three Waste ", makes the annual underproduction of grain
10000000000 kilograms.The area that exceeds standard at elements such as some area hydrargyrum, arsenic, copper, zinc accounts for the half polluting the gross area
Left and right.Some vegetables base Cadmium Pollution in Soils are universal, in the main facilities vegetable gardening field, suburbs wherein having,
Zinc content in soil is up to 517 mg/kg, exceeds standard 5 times more than.Owing to soil pollution has disguise,
Latency and chronicity, its serious consequence is only capable of being worked the mischief to animals and humans health by food, thus not
Easily discovered by people.Particularly to have mobility little for the heavy metal in soil, and the time of polluting is long, by physics or
Chemical method is difficult to the features such as thorough removing.Improve the ecological environment, soil protection quality, control and rehabilitating soil
Polluting, could realize agricultural safety, this is a sternness facing at present of China and urgent significant problem.
At present, the processing method of pollution by pesticides and heavy metal pollution is a lot, sum up include chemical treatment,
Burn, bury and the method such as bioremediation.Chemical treatment method feasibility is strong, but produces in processing procedure
Substantial amounts of bronsted lowry acids and bases bronsted lowry, these materials must also add process again.It is the most reliable that burning is probably these pollutant of degraded
Method.But, processing procedure is released substantial amounts of poison, and needs substantial amounts of heat energy.Bury and also deposit
In problem, it is mainly manifested in and soil about and water source are caused secondary pollution.Therefore, exploitation is a kind of energy-conservation
The processing method of environmental protection low cost is extremely urgent.
Summary of the invention
An object of the present invention is to provide a kind of method processing sewage Pesticide Residues and heavy metal Hg.
The present invention adopts the following technical scheme that realization:
A kind of method processing sewage Pesticide Residues and heavy metal Hg, comprises the steps:
(1) pretreatment: by filtering and precipitation, remove the float in handled sewage；
(2) main process: foul water fraction adds medicament ammonium sulfide according to hydrargyrum concentration in water, flocculation aid (gathers
Acrylamide) and flocculant (aluminium polychlorid), join in sewage, under normal temperature condition, flowing training
Support 24 hours, hydrargyrum in sewage can be removed；
(3) post processing: sewage again passes by zeolite and activated carbon adsorption tank, adsorbs pesticide residues；
(4) will process after sewage sterile-processed after, directly discharge or reuse.
Another technical scheme that the present invention provides is:
A kind of method processing sewage Pesticide Residues and heavy metal Hg, comprises the steps:
(1) pretreatment: by filtering and precipitation, remove the float in handled sewage；
(2) main process: the antibacterial pseudomonas putida (Pseudomonas to exponential phase will be cultivated
Putida), joining in sewage, under normal temperature condition, flowing is cultivated 24 hours, can remove dirt
Hydrargyrum in water；
(3) post processing: sewage again passes by zeolite and activated carbon adsorption tank, adsorbs pesticide residues；
(4) will process after sewage sterile-processed after, directly discharge or reuse.
Preferably, described pseudomonas putida is for having proceeded to chtochrome oxidase reductase (P450 CYP9G2)
Pseudomonas with the gene of modified NADPH-cytochrome P450 reductase (POR)
Putida SPl CGMCC N0.3887 bacterial strain, described chtochrome oxidase reductase (P450 CYP9G2)
The protein sequence of coded by said gene sees GenBank:AB096739, described modified NADPH-
The protein sequence of the coded by said gene of cytochrome P450 reductase (POR) is relative to NADPH-cell
Cytochrome p 450 reductase (POR) original amino acid suddenly change the most as follows Asp44Ser (represent
The Asp of the 44th sports Ser), Gln74Tyr, Leu96Lys, Asp137Ala, His199Gly,
Ile231Gly, Asp296Glu, Asp346Glu, Pro381Cys, Tyr484Lys, Ser519Arg,
The original protein sequences of Gln614His, NADPH-cytochrome P450 reductase (POR) coded by said gene
See GenBank:L19897.
A series of bacterial strains through genetic modification that the present invention provides not only have the effect preferably going the removal of mercury, with
Time can also effectively remove the pesticide residues in sewage, there is preferable application prospect.
Said method has the advantages that
1, by the processing method of the present invention, substantially increase the treatment effect of hydrargyrum and pesticide residues, reduce
Response time.
2, the conversion bacterial strain of a kind of degrade hydrargyrum and pesticide residues is provided, there is higher removal efficiency.
By specific embodiment, the present invention will be described in detail in order to make it easy to understand, following.Need spy
Not it is noted that instantiation is merely to explanation, it is not intended that limitation of the scope of the invention.Obviously ability
The present invention can be made various according to illustrating herein by the those of ordinary skill in territory within the scope of the invention
Correction and change, these revise and change also include in the scope of the present invention.
Detailed description of the invention
Embodiment 1 hydrargyrum and the process of pesticide residues sewage
During wastewater influent, the concentration of hydrargyrum is 30mg/L, Rogor 20mg/L, fenifrothion 20mg/L, Methomyl
20mg/L。
Pretreatment: by filtering and precipitation, remove the float in handled sewage；
Main process: the antibacterial pseudomonas putida disclosed in the CN 102373161A to exponential phase will be cultivated
Pseudomonas putida SPl CGMCC N0.3887, joins in sewage, under normal temperature condition, and flowing
Cultivate 24 hours, hydrargyrum in sewage can be removed；
Post processing: sewage again passes by zeolite and activated carbon adsorption tank overnight processes, adsorbs pesticide residues；
Will process after sewage sterile-processed after, directly discharge or reuse.After testing, the concentration of hydrargyrum is
2.2mg/L, Rogor 15.4mg/L, fenifrothion 14.3mg/L, Methomyl 16.4mg/L.
The transformation of embodiment 2 degrading pesticide gene and acquisition
According to the method disclosed in CN1970741A, clone obtains CYP9G2 and POR gene.For POR
Gene has carried out following transformation, by multiplex PCR, (corresponding Asp44Ser being represented, the Asp of the 44th dashes forward
Become Ser), Gln74Tyr, Leu96Lys, Asp137Ala, His199Gly, Ile231Gly, Asp296Glu,
Gly316Asn, Asp346Glu, Pro381Cys, Tyr484Lys, Ser519Arg, Gln614His, mutational site
It is introduced between in albumen shown in GenBank:L19897, thus constructs different mutant POR genes
(preparation method referring to prior art can obtain).By the POR gene after sudden change and CYP9G2 gene
It is connected respectively at expression vector according to the method preparing CN1970741A together, the recombiant plasmid that will be proved to be successful
Convert and enter in Pseudomonas putida SPl CGMCC N0.3887 bacterial strain, detected by PCR, its
Successfully construct.
The checking of embodiment 3 sewage degradation effect
By the bacterial strain containing different mutains, the most all according to the method for embodiment 1, the checking place to sewage
Reason effect.During wastewater influent, the concentration of hydrargyrum is 30mg/L, Rogor 20mg/L, and fenifrothion 20mg/L goes out many
Prestige 20mg/L.To proceed to the bacterium of the bacterial strain sum of unmutated CYP9G2 and POR gene as comparison.Concrete outcome
As follows:
From above result it can be seen that imported the bacterium of POR and the CYP9G2 gene mutation of specific sudden change
Having the effect removing the removal of mercury and pesticide of enhancing, this absolutely proves not to be that arbitrary change all has preferably effect
Really.Applicant just obtains several sites with better effects by substantial amounts of checking.
Claims (4)
1. the method processing sewage Pesticide Residues and heavy metal Hg, comprises the steps:
(1) pretreatment: by filtering and precipitation, remove the float in handled sewage；
(2) main process: foul water fraction adds medicament ammonium sulfide, polyacrylamide and aluminium polychlorid according to hydrargyrum concentration in water, joins in sewage, under normal temperature condition, flowing is cultivated 24 hours, can remove hydrargyrum in sewage；
(3) post processing: sewage again passes by zeolite and activated carbon adsorption tank, adsorbs pesticide residues；
(4) will process after sewage sterile-processed after, directly discharge or reuse.
2. the method processing sewage Pesticide Residues and heavy metal Hg, comprises the steps:
(1) pretreatment: by filtering and precipitation, remove the float in handled sewage；
(2) main process: by cultivating the antibacterial pseudomonas putida (Pseudomonas putida) to exponential phase, join in sewage, under normal temperature condition, flowing is cultivated 24 hours, can remove hydrargyrum in sewage；
(3) post processing: sewage again passes by zeolite and activated carbon adsorption tank, adsorbs pesticide residues；
(4) will process after sewage sterile-processed after, directly discharge or reuse.
null3. method as claimed in claim 2，It is characterized in that: described pseudomonas putida is the Pseudomonas putida SPl CGMCC N0.3887 bacterial strain of the gene having proceeded to chtochrome oxidase reductase (P450CYP9G2) and modified NADPH-cytochrome P450 reductase (POR)，The protein sequence of described chtochrome oxidase reductase (P450CYP9G2) coded by said gene sees GenBank:AB096739，The protein sequence of the coded by said gene of described modified NADPH-cytochrome P450 reductase (POR) is for suddenling change as follows Asp137Ala relative to NADPH-cytochrome P450 reductase (POR) original amino acid，The original protein sequences of NADPH-cytochrome P450 reductase (POR) coded by said gene sees GenBank:L19897.
4. a protein variant, it is characterised in that: described variant sequence thereof is for suddenling change as follows Asp137Ala relative to albumen original amino acid, and albumen original protein sequences sees GenBank:L19897.
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| CN105923916B (en) | 2018-11-02 |
| CN105836933B (en) | 2018-12-21 |
| CN105923912A (en) | 2016-09-07 |
| CN105923837A (en) | 2016-09-07 |
| CN105836980A (en) | 2016-08-10 |
| CN105836980B (en) | 2019-05-10 |
| CN105923837B (en) | 2019-06-11 |
| CN105836933A (en) | 2016-08-10 |
| CN106045197B (en) | 2019-05-03 |
| CN105923912B (en) | 2019-05-07 |
| CN105923914B (en) | 2018-10-09 |
| CN105923914A (en) | 2016-09-07 |
| CN104876371B (en) | 2017-01-04 |
| CN105923917B (en) | 2018-11-02 |
| CN105836981B (en) | 2018-10-30 |
| CN105836981A (en) | 2016-08-10 |
| CN105923917A (en) | 2016-09-07 |
| CN105923916A (en) | 2016-09-07 |
| CN104876371A (en) | 2015-09-02 |
| CN105836983B (en) | 2018-10-16 |
| CN105836982B (en) | 2018-10-30 |
| CN106045197A (en) | 2016-10-26 |
| CN105836983A (en) | 2016-08-10 |
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