CN106035329A - Compound bactericide for preventing and controlling grape anthracnose and application thereof - Google Patents
Compound bactericide for preventing and controlling grape anthracnose and application thereof Download PDFInfo
- Publication number
- CN106035329A CN106035329A CN201610364867.4A CN201610364867A CN106035329A CN 106035329 A CN106035329 A CN 106035329A CN 201610364867 A CN201610364867 A CN 201610364867A CN 106035329 A CN106035329 A CN 106035329A
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- China
- Prior art keywords
- anthracnose
- grape
- preventing
- flutolanil
- treating
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/02—Cultivation of hops or vines
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof
- A01N37/22—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof the nitrogen atom being directly attached to an aromatic ring system, e.g. anilides
- A01N37/24—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof the nitrogen atom being directly attached to an aromatic ring system, e.g. anilides containing at least one oxygen or sulfur atom being directly attached to the same aromatic ring system
Abstract
The invention discloses a compound bactericide for preventing and controlling grape anthracnose. The compound bactericide for preventing and controlling grape anthracnose is wettable powder, a colloidal suspension agent, a micro-emulsion, water dispersible granules or granules containing cyanalkenylesbiothrin and flutotanil, wherein the mass ratio of the cyanalkenylesbiothrin to the flutotanil is 1 to1-5, the total effective dosages of the cyanalkenylesbiothrin to the flutotanil per hectare are 30 g and 30-150 g respectively, the water adding amount is 450-600 kg, and the compound bactericide is sprayed at each stage for one time before a grape germination stage, a shoot vigorous-growth stage, a primary flowering stage, an early berry forming stage and a fruit enlargement stage or bagging and can effectively delay the bactericide resistance of the grape anthracnose. The blended compound bactericide is higher than a single agent in prevention and control effect, and the compound bactericide has the advantages of being low in cost and simple to process.
Description
Technical field
The invention belongs to pesticide sterilizing composite, particularly relate to a kind of composite bactericide preventing and treating bitter rot or anthracnose of grape and answer
With.
Background technology
Bitter rot or anthracnose of grape (Grape anthracnose) has another name called late blight, is one of the important disease during Fructus Vitis viniferae produces.Charcoal
Cellulitis disease is Deuteromycotina, colletotrichum colletotrichum gloeosporioides Penz (Colletotrichum
Gloeosporioides Penz) infect and cause.Pathogenic bacteria mainly with mycelium tree body in annual branches and tendrils
In survive the winter.Spring in next year propagates in a large number with wind and rain, hides in the young sprout infected, young fruit.Typically occur in coloring or closely become
On ripe fruit, cause fruit to rot in a large number when falling ill serious, cause serious loss.
The chemical agent preventing and treating bitter rot or anthracnose of grape conventional mainly has Mancozeb, thiram, carbendazim etc..For a long time,
Owing to many frequencys, multiple dose use chemical agent, cause grape anthracnose that conventional chemical agent is all created in various degree
Anti-(resistance to) property of medicine, production occurs example that preventing and treating is failed repeatedly.In order to delay the Drug resistance of conventional higher effective and lower toxic pesticide, prolong
In the service life of long pesticide, the mixture that existing medicament carries out science is the most necessary.
Summary of the invention
In order to overcome the deficiencies in the prior art, it is an object of the invention to provide one and bitter rot or anthracnose of grape is had high anti-
Control cyanogen alkene chrysanthemum ester and the flutolanil composite bactericide of effect.
For reaching above-mentioned purpose, the present invention is achieved by the following technical solutions.
A kind of composite bactericide preventing and treating bitter rot or anthracnose of grape, comprise cyanogen alkene chrysanthemum ester, flutolanil by common method prepare can
WP, colloidal suspending agent, microemulsion, water dispersion granule or granule.
The most described cyanogen alkene chrysanthemum ester is 1:1~5 with the mass ratio of flutolanil.
The most described cyanogen alkene chrysanthemum ester is 1:4 with the mass ratio of flutolanil.
When making wettable powder, its proportioning is by mass percentage further: cyanogen alkene chrysanthemum ester 1~10%, flutolanil
1~50%, conven-tional adjuvants 5~15%, surplus be inserts.Described auxiliary agent is in emulsifying agent, dispersant, stabilizer, penetrating agent
One or more.
The most described inserts is Kaolin.
Additionally provide a kind of composite bactericide preventing and treating bitter rot or anthracnose of grape further and prevent and treat answering in bitter rot or anthracnose of grape
With, it is characterised in that the consumption of 30g and 30~150g it is respectively according to cyanogen alkene chrysanthemum ester in per hectare and the total effective dose of flutolanil,
It is watered 450~600kg, carefully sprays.
Further at Fructus Vitis viniferae budding period, young sprout prosperous long-term, initial bloom stage, berry generation initial stage and fruit expanding period or bagging
Before, each phase sprays 1 time.
Beneficial effect: compared with prior art, it is an advantage of the current invention that: in compound drug, cyanogen alkene chrysanthemum ester and flutolanil divide
Not Wei the antibacterial of the different mechanisms of action, can effectively delay the Drug resistance of grape anthracnose;After mixture, prevention effect is significantly high
In single dose, and there is low cost and process easy advantage.
Detailed description of the invention
Below in conjunction with test, the present invention is described in further detail.
The toxicity of Strawberry anthracnose bacterium is determined as follows by the cyanogen alkene chrysanthemum ester of the present invention and flutolanil composite bactericide:
Cyanogen alkene chrysanthemum ester and the former medicinal acetone of flutolanil are dissolved into 1000 μ g/ml mother solutions respectively, and add 1% tween 80.With
Sterilized water is configured to concentration and is respectively the single dose series medicinal liquid of 200,100,20,10,2,1,0.2 and 0.1 μ g/mL, is placed in triangle
In Ping standby.By flutolanil: cyanogen alkene chrysanthemum ester prepare respectively by effective content become 1:1,2:1,3:1,4:1 and 5:1 mixture be
Row medicinal liquid, is placed in triangular flask standby.Take above-mentioned serial dilution medicament 1mL medicinal liquid respectively with 9mL culture medium (PDA) at culture dish
Interior mixing, makes the PDA culture medium that content of dispersion in culture dish is 20,10,2,1,0.2,0.1,0.02 and 0.01 μ g/mL, by nothing
Bacterium water makees blank.The grape anthracnose of preservation is transferred in PDA culture medium, activated after, use at nearly colony edge
The bacterium cake of a diameter of 4mm produced by card punch, then in the flat culture medium of PDA that is transferred to band medicine and blank, often processes repetition 4
Secondary.Light culture 4d at 25 DEG C.
When bacterium colony covers with plate soon in blank, decussation method is used to measure colony diameter.Calculate colony diameter
Meansigma methods, and calculate mycelial growth average inhibition according to the following formula: mycelial growth average inhibition=[(comparison bacterium colony is straight
Footpath average-process colony diameter average)/(comparison colony diameter average-inoculation bacterium cake diameter)] × 100%.Use DPS data
Processing system, calculates regression equation, EC50With EC90 and 95% confidence limit thereof.By Wadley method, calculate coefficient of synergism (SR).
Evaluate the synergy type of pharmacy mix according to coefficient of synergism (SR), i.e. SR < 0.5 is antagonism, 0.5≤SR
≤ 1.5 is summation action, and SR > 1.5 is potentiation.SR=EC50(Eth)/EC50(Eob), EC50(Eth)=(a+b)/[(a/
EC50A)+(b/EC50B)].Wherein, A, B are respectively antibacterial single dose, and a, b are corresponding single dose ratio in mixture, EC50
(Eth) it is mixture EC50Theoretical value, EC50(Eob) it is mixture EC50Measured value.
The Toxicity Determination result of grape anthracnose is shown by complex preparation and single dose, flutolanil, cyanogen alkene chrysanthemum ester pair
The EC of grape anthracnose50Being respectively 1.421 μ g/mL and 0.1087 μ g/mL, cyanogen alkene chrysanthemum ester is to grape anthracnose indoor biological
Activity high (table 1).
Flutolanil and cyanogen alkene chrysanthemum ester are pressed 1:1,2:1,3:1,4:1 and 5:1 equal proportion mixture respectively and are combined bitter rot or anthracnose of grape
The EC of bacterium50Respectively 0.2148 μ g/mL, 0.1105 μ g/mL, 0.1039 μ g/mL, 0.0547 μ g/mL and 0.3487 μ g/mL, 5 kinds
Mixture combination is respectively 1.15,1.67,1.42,2.78 and 0.41 to the coefficient of synergism (SR) of grape anthracnose.Flutolanil with
Cyanogen alkene chrysanthemum ester is extremely notable (coefficient of synergism SR reaches 2.78) to the potentiation of grape anthracnose in the ratio mixture of 4:1.
Table 1 cyanogen alkene chrysanthemum ester, flutolanil and the built agent toxicity test to bitter rot or anthracnose of grape
The cyanogen alkene chrysanthemum ester of the present invention and the flutolanil composite bactericide field test to Strawberry anthracnose bacterium:
(1) test Fructus Vitis viniferae is open country planting patterns, and spraying time is budding period, prosperous long-term, initial bloom stage, the berry life of young sprout
Long initial stage and fruit expanding period (the 3-6 month in 2014), altogether preventing and treating 5 times.Wherein 25% cyanogen alkene chrysanthemum ester flutolanil WP used contains
Cyanogen alkene chrysanthemum ester 5%, flutolanil 20%, wetting agent 10%, K12 (sodium lauryl sulphate) 2%, remaining is inserts Kaolin.
Water consumption is 600L/hm2, prevention and controls is thin spraying.
Experimental concentration is designed as:
Process (1) 25% cyanogen alkene chrysanthemum ester flutolanil WP 150g.ai/hm2Prevent and treat 5 times;
Process (2) 25% cyanogen alkene chrysanthemum ester WP 150g.ai/hm2Prevent and treat 5 times;
Process (3) 25% flutolanil WP 150g.ai/hm2Prevent and treat 5 times;
Process (4) clear water.
Field control result shows, open country Fructus Vitis viniferae is not in the case of preventing and treating, and anthrax sickness rate is the highest, disease index
The highest.With 25% cyanogen alkene chrysanthemum ester flutolanil, 25% cyanogen alkene chrysanthemum ester and 25% flutolanil WP 150g.ai/hm2Prevent and treat 5 times
After prevention effect respectively reach 96.89%, 74.03% and 68.47%, mixed after field potentiation extremely notable (table 2).
The table 2 25% flutolanil flutolanil WP field control result to bitter rot or anthracnose of grape
(2) test Fructus Vitis viniferae is open country planting patterns, and spraying time is budding period, prosperous long-term, initial bloom stage, the berry life of young sprout
Long initial stage and fruit expanding period (the 3-6 month in 2014), use the cyanogen alkene chrysanthemum ester of different ratio and flutolanil composite bactericide to prevent altogether
Control 5 times.
Antibacterial (1): containing cyanogen alkene chrysanthemum ester 5%, flutolanil 20%, wetting agent in 25% cyanogen alkene chrysanthemum ester flutolanil WP
10%, K12 (sodium lauryl sulphate) 2%, remaining is inserts Kaolin, and water consumption is 600L/hm2;
Antibacterial (2): in 2% cyanogen alkene chrysanthemum ester flutolanil WP containing cyanogen alkene chrysanthemum ester 1%, flutolanil 1%, wetting agent 4%,
K12 (sodium lauryl sulphate) 1%, remaining is inserts Kaolin, and water consumption is 450L/hm2;
Antibacterial (3): containing cyanogen alkene chrysanthemum ester 10%, flutolanil 50%, wetting agent in 60% cyanogen alkene chrysanthemum ester flutolanil WP
10%, K12 (sodium lauryl sulphate) 5%, remaining is inserts Kaolin, and water consumption is 600L/hm2;
Field control result the results are shown in Table 3.
The table 3 different ratio flutolanil flutolanil WP field control result to bitter rot or anthracnose of grape
The present invention is illustrated according to above-described embodiment, it will be appreciated that above-described embodiment limits this most in any form
Invention, the technical scheme that all employing equivalents or equivalent transformation mode are obtained, within all falling within protection scope of the present invention.
Claims (7)
1. the composite bactericide preventing and treating bitter rot or anthracnose of grape, it is characterised in that comprise cyanogen alkene chrysanthemum ester, the wettable powder of flutolanil
Agent, colloidal suspending agent, microemulsion, water dispersion granule or granule.
A kind of composite bactericide preventing and treating bitter rot or anthracnose of grape the most according to claim 1, it is characterised in that described cyanogen alkene chrysanthemum
Ester is 1:1 ~ 5 with the mass ratio of flutolanil.
A kind of composite bactericide preventing and treating bitter rot or anthracnose of grape the most according to claim 1, it is characterised in that described cyanogen alkene chrysanthemum
Ester is 1:4 with the mass ratio of flutolanil.
A kind of composite bactericide preventing and treating bitter rot or anthracnose of grape the most according to claim 1, it is characterised in that wettable when making
During property powder, its proportioning is by mass percentage: cyanogen alkene chrysanthemum ester 1~10%, flutolanil 1~50%, conven-tional adjuvants 5~15%, surplus
For inserts.
A kind of composite bactericide preventing and treating bitter rot or anthracnose of grape the most according to claim 4, it is characterised in that described inserts
For Kaolin.
6. the composite bactericide preventing and treating bitter rot or anthracnose of grape is in the application preventing and treating in bitter rot or anthracnose of grape, it is characterised in that according to
In per hectare, cyanogen alkene chrysanthemum ester and the total effective dose of flutolanil are respectively 30 g and the consumption of 30 ~ 150 g, are watered 450~600 kg, carefully
Spraying.
A kind of composite bactericide preventing and treating bitter rot or anthracnose of grape the most according to claim 6 is preventing and treating answering in bitter rot or anthracnose of grape
With, it is characterised in that at Fructus Vitis viniferae budding period, young sprout prosperous long-term, initial bloom stage, berry generation initial stage and fruit expanding period or bagging
Before, each phase sprays 1 time.
Priority Applications (1)
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CN201610364867.4A CN106035329A (en) | 2016-05-27 | 2016-05-27 | Compound bactericide for preventing and controlling grape anthracnose and application thereof |
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CN201610364867.4A CN106035329A (en) | 2016-05-27 | 2016-05-27 | Compound bactericide for preventing and controlling grape anthracnose and application thereof |
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Cited By (2)
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CN110881480A (en) * | 2018-09-06 | 2020-03-17 | 浙江泛亚生物医药股份有限公司 | Trichoderma harzianum biological agent for preventing and treating wheat scab |
CN114503842A (en) * | 2022-02-28 | 2022-05-17 | 贵阳学院 | Blueberry anthracnose control method |
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2016
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Patent Citations (3)
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN110881480A (en) * | 2018-09-06 | 2020-03-17 | 浙江泛亚生物医药股份有限公司 | Trichoderma harzianum biological agent for preventing and treating wheat scab |
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CN114503842A (en) * | 2022-02-28 | 2022-05-17 | 贵阳学院 | Blueberry anthracnose control method |
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