CN106018517B - The preparation of a kind of complex film modified electrode of tartaric acid-graphene quantum dot and applied to identification Tryptophan enantiomer - Google Patents
The preparation of a kind of complex film modified electrode of tartaric acid-graphene quantum dot and applied to identification Tryptophan enantiomer Download PDFInfo
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- 229910021389 graphene Inorganic materials 0.000 title claims abstract description 74
- 239000002096 quantum dot Substances 0.000 title claims abstract description 68
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical class C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 title claims abstract description 28
- 238000002360 preparation method Methods 0.000 title claims abstract description 21
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 44
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 claims abstract description 38
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 claims abstract description 28
- 239000011975 tartaric acid Substances 0.000 claims abstract description 18
- 235000002906 tartaric acid Nutrition 0.000 claims abstract description 16
- 238000000034 method Methods 0.000 claims abstract description 13
- 239000001358 L(+)-tartaric acid Substances 0.000 claims abstract description 11
- 235000011002 L(+)-tartaric acid Nutrition 0.000 claims abstract description 11
- FEWJPZIEWOKRBE-LWMBPPNESA-N L-(+)-Tartaric acid Natural products OC(=O)[C@@H](O)[C@H](O)C(O)=O FEWJPZIEWOKRBE-LWMBPPNESA-N 0.000 claims abstract description 11
- AUONNNVJUCSETH-UHFFFAOYSA-N icosanoyl icosanoate Chemical compound CCCCCCCCCCCCCCCCCCCC(=O)OC(=O)CCCCCCCCCCCCCCCCCCC AUONNNVJUCSETH-UHFFFAOYSA-N 0.000 claims abstract description 11
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 claims description 18
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 claims description 13
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Natural products OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 13
- 229910052799 carbon Inorganic materials 0.000 claims description 10
- 229910052697 platinum Inorganic materials 0.000 claims description 9
- ZOMNIUBKTOKEHS-UHFFFAOYSA-L dimercury dichloride Chemical class Cl[Hg][Hg]Cl ZOMNIUBKTOKEHS-UHFFFAOYSA-L 0.000 claims description 8
- 238000002474 experimental method Methods 0.000 claims description 8
- 229940075397 calomel Drugs 0.000 claims description 6
- 239000002131 composite material Substances 0.000 claims description 5
- 238000004090 dissolution Methods 0.000 claims description 5
- 239000012528 membrane Substances 0.000 claims description 5
- 239000007787 solid Substances 0.000 claims description 5
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 5
- 239000012498 ultrapure water Substances 0.000 claims description 5
- 238000002484 cyclic voltammetry Methods 0.000 claims description 2
- 230000005611 electricity Effects 0.000 claims description 2
- 238000004070 electrodeposition Methods 0.000 claims description 2
- 230000035484 reaction time Effects 0.000 claims description 2
- 238000004062 sedimentation Methods 0.000 claims description 2
- PXRKCOCTEMYUEG-UHFFFAOYSA-N 5-aminoisoindole-1,3-dione Chemical compound NC1=CC=C2C(=O)NC(=O)C2=C1 PXRKCOCTEMYUEG-UHFFFAOYSA-N 0.000 claims 2
- 210000001367 artery Anatomy 0.000 claims 1
- 239000007788 liquid Substances 0.000 claims 1
- 238000004080 punching Methods 0.000 claims 1
- 210000003462 vein Anatomy 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 18
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 10
- 229960001367 tartaric acid Drugs 0.000 description 8
- 230000000052 comparative effect Effects 0.000 description 6
- 150000001413 amino acids Chemical class 0.000 description 5
- 229910021529 ammonia Inorganic materials 0.000 description 5
- 235000015165 citric acid Nutrition 0.000 description 5
- 238000000151 deposition Methods 0.000 description 4
- 230000008021 deposition Effects 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- ZLWLTDZLUVBSRJ-UHFFFAOYSA-K chembl2360149 Chemical compound [Na+].[Na+].[Na+].O=C1C(N=NC=2C=CC(=CC=2)S([O-])(=O)=O)=C(C(=O)[O-])NN1C1=CC=C(S([O-])(=O)=O)C=C1 ZLWLTDZLUVBSRJ-UHFFFAOYSA-K 0.000 description 3
- 125000004122 cyclic group Chemical group 0.000 description 3
- 238000009738 saturating Methods 0.000 description 3
- 238000013507 mapping Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000004575 stone Substances 0.000 description 2
- 150000003892 tartrate salts Chemical class 0.000 description 2
- HBAQYPYDRFILMT-UHFFFAOYSA-N 8-[3-(1-cyclopropylpyrazol-4-yl)-1H-pyrazolo[4,3-d]pyrimidin-5-yl]-3-methyl-3,8-diazabicyclo[3.2.1]octan-2-one Chemical class C1(CC1)N1N=CC(=C1)C1=NNC2=C1N=C(N=C2)N1C2C(N(CC1CC2)C)=O HBAQYPYDRFILMT-UHFFFAOYSA-N 0.000 description 1
- QIVBCDIJIAJPQS-SECBINFHSA-N D-tryptophane Chemical compound C1=CC=C2C(C[C@@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-SECBINFHSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 239000000090 biomarker Substances 0.000 description 1
- 150000001732 carboxylic acid derivatives Chemical class 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 229960001270 d- tartaric acid Drugs 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- 239000011859 microparticle Substances 0.000 description 1
- 239000002858 neurotransmitter agent Substances 0.000 description 1
- 231100000956 nontoxicity Toxicity 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000000870 ultraviolet spectroscopy Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/48—Systems using polarography, i.e. measuring changes in current under a slowly-varying voltage
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Abstract
The present invention relates to a kind of preparation of complex film modified electrode of tartaric acid graphene quantum dot and applied to identification Tryptophan enantiomer.Include the following steps：The preparation of graphene quantum dot, the preparation of the complex film modified electrode of tartaric acid graphene quantum dot, electrochemical process identify Tryptophan enantiomer.The beneficial effects of the invention are as follows：The complex film modified electrode preparation method of tartaric acid graphene quantum dot is simple, it makes full use of tartaric acid that there is chiral environment (being respectively L (+) tartaric acid and D () tartaric acid) this characteristic, the excellent complex film modified electrode of tartaric acid graphene quantum dot of recognition performance is prepared in conjunction with graphene quantum dot.The complex film modified electrode possesses more excellent recognition effect than single tartaric acid or modified by graphene quantum dot electrode.
Description
Technical field
The present invention relates to a kind of preparation of complex film modified electrode of tartaric acid-graphene quantum dot and applied to identification color ammonia
Sour enantiomer belongs to biotechnology and electrochemical research field.
Technical background
Amino acid is a kind of organic compound beneficial to human body, for example, inside protein just containing there are many amino acid at
Part.The effect of amino acid, adjusts function of human body with the protein needed for effect mainly supplement human life, resistance building,
It sustains life.It can be seen that amino acid is the first element of human body.Tryptophan is neurotransmitter -5- hydroxyl colors important in human body
The precursor of amine is one of amino acid needed by human, and there are two types of the chiral enantiomers of various configuration, respectively L-Trp and D-
Tryptophan.Different stereochemical structures will produce different pharmacological action and reaction, so identification L-/D- tryptophans are most important.
The method of detection tryptophan has high performance liquid chromatography, ultraviolet spectroscopy, fluorescent spectrometry etc. at present, but at these method samples
Reason process is cumbersome.
Graphene is two-dimensional structure, there is its unique performance.Its specific surface area is larger, have high current-carrying mobility,
It is very thin and dense, in biological cell the features such as nontoxicity.It is that quasi-zero dimension is received for graphene quantum dot is compared to graphene
Rice material, and have the property of graphene, size is in 10nm hereinafter, therefore its quantum confined effect and boundary effect are brighter
It is aobvious.Due to quantum confined effect and boundary effect, the shapes and sizes of graphene quantum dot by determine they electricity, optics,
Magnetic and chemical characteristic.It is mainly used in solar cell, electronic equipment, optical dye, biomarker and multiple microparticles system
Etc..
The present invention selects tartaric acid enantiomer as chiral material, and tartaric acid is a kind of carboxylic acid, is present in various plants,
It is also one of main organic acid in grape wine.L-/D- tartaric acid contains a large amount of hydroxyls with coordination ability, provides chiral work
Property site, can with the amino in tryptophan formed hydrogen bond, be theoretically utilized in electrochemical recognition Tryptophan enantiomer.The present invention
It is combined tartaric acid enantiomer to form the composite membrane with stereoselectivity with graphene quantum dot, can identify tryptophan with mapping
Isomers.
Invention content
The present invention is intended to provide a kind of preparation method of the complex film modified electrode of tartaric acid-graphene quantum dot, and by its
Tryptophan enantiomer is identified applied to electrochemical process.By L- (+)-tartaric acid-graphene quantum dot composite membrane or D- (-)-winestone
The complex film modified efficiency that mapping identification L-/D- tryptophans can be effectively improved after glass-carbon electrode of acid-graphene quantum dot.
A kind of preparation of complex film modified electrode of tartaric acid-graphene quantum dot of the present invention and applied to identification color ammonia
Sour enantiomer, includes the following steps：
A, graphene quantum dot is prepared：Citric acid is added in crucible, is heated in program-controlled cabinet-type electric furnace, reaction knot
Solid sample is taken out after beam, yellow transparent solution is filtered to obtain, in room temperature after product fully dissolves with ultra-pure water ultrasonic dissolution
Under be kept in dark place；
B, the complex film modified electrode of tartaric acid-graphene quantum dot is prepared：It tests using three-electrode system, glass-carbon electrode is
Working electrode, platinum plate electrode are to electrode, and saturated calomel electrode is reference electrode, is dipped in graphene quantum dot solution,
Within the scope of the electrochemical window of 0V~1.0V (vs.SCE), carries out cyclic voltammetry scan and obtains modified by graphene quantum dot electrode,
The modified electrode is immersed in prepared L- (+)-tartaric acid or D- (-)-tartaric acid solution again, potentiostatic method is prepared into winestone
The complex film modified electrode of acid-graphene quantum dot；
C, electrochemical process identifies Tryptophan enantiomer：Experiment uses three-electrode system, L- (+)-tartaric acid-graphene quantum
Point or D- (-)-tartaric acid-complex film modified electrode of graphene quantum dot are working electrode, and platinum plate electrode is to electrode, and saturation is sweet
Mercury electrode is reference electrode, it is immersed respectively in the L-/D- tryptophan solutions configured, and differential pulse is carried out after standing and is swept
It retouches.
A kind of preparation of complex film modified electrode of tartaric acid-graphene quantum dot of the present invention and applied to identification color ammonia
Sour enantiomer, further technical solution can also be in the step a that citric acid quality is 0.1~15.0g, reaction temperature
It it is 50~400 DEG C, the reaction time is 0.1~3h.
A kind of preparation of complex film modified electrode of tartaric acid-graphene quantum dot of the present invention and applied to identification color ammonia
Sour enantiomer, further technical solution can also be in the step b that glass-carbon electrode scans in graphene quantum dot solution
The number of turns is 4~100 circles, configures the tartaric acid solution of 0.1g/mL, and sedimentation potential is+1.0V, and electrodeposition time is 10~1000s.
A kind of preparation of complex film modified electrode of tartaric acid-graphene quantum dot of the present invention and applied to identification color ammonia
Sour enantiomer, further technical solution can also be in the step c, L- (+)-tartaric acid-graphene quantum dot or D-
The complex film modified electrode of (-)-tartaric acid-graphene quantum dot immerse respectively L-/D- tryptophan solutions a concentration of 0.1~
10mmol/L。
The beneficial effects of the invention are as follows：The complex film modified electrode preparation method of tartaric acid-graphene quantum dot is simple, fully
There is chiral environment (being respectively L- (+)-tartaric acid and D- (-)-tartaric acid) this characteristic using tartaric acid, in conjunction with graphene amount
Sub- point prepares the excellent complex film modified electrode of tartaric acid-graphene quantum dot of recognition performance.The complex film modified electrode is than single
One tartaric acid or modified by graphene quantum dot electrode possess more excellent recognition effect.
Description of the drawings
The following further describes the present invention with reference to the drawings.
Fig. 1 is the transmission electron microscope picture of graphene quantum dot in embodiment one, two；
Fig. 2 is the complex film modified electrode tryptophan enantiomer of L- (+)-tartaric acid-graphene quantum dot in embodiment one
Recognition effect figure；
Fig. 3 is the complex film modified electrode tryptophan enantiomer of D- (-)-tartaric acid-graphene quantum dot in embodiment two
Recognition effect figure；
Fig. 4 is the recognition effect figure of modified by graphene quantum dot electrode tryptophan enantiomer in comparative example one；
Fig. 5 is the recognition effect figure of L- (+)-tartaric acid modified electrode tryptophan enantiomer in comparative example two；
Fig. 6 is the recognition effect figure of D- (-)-tartaric acid modified electrode tryptophan enantiomer in comparative example three.
Specific implementation mode
Presently in connection with specific embodiment, the present invention will be further described, following embodiment be intended to illustrate invention rather than
Limitation of the invention further.
Embodiment one：
It includes following steps to prepare L- (+)-complex film modified electrode of tartaric acid-graphene quantum dot：
(1) 2.0g citric acids are weighed to be added in 10mL crucibles, heat reaction under the conditions of 200 DEG C in program-controlled cabinet-type electric furnace
30min takes out solid sample, with ultra-pure water ultrasonic dissolution after reaction.After product fully dissolves, filter yellow is saturating
Bright solution, is kept in dark place at normal temperatures.
(2) it is working electrode that experiment, which uses three-electrode system, glass-carbon electrode (diameter 3mm), and platinum plate electrode is to satisfy to electrode
It is reference electrode with calomel electrode (SCE), is dipped in graphene quantum dot solution, in the electrification of 0V~1.0V (vs.SCE)
It learns in window ranges, cyclic voltammetric 50 is swept with the speed of 0.1V/s and is enclosed, modified by graphene quantum dot electrode is obtained.Again by the modification
Electrode immerses in 0.1g/mL L- (+)-tartaric acid solution prepared, with the potential deposition 400s of potentiostatic method application+1.0V, system
It is standby to obtain L- (+)-tartaric acid-complex film modified electrode of graphene quantum dot.
L- (+)-tartaric acid of preparation-complex film modified electrode of graphene quantum dot is immersed into a concentration of of configuration respectively
In 0.5mmol/L L-/D- tryptophan solutions, the recognition effect figure of Tryptophan enantiomer is shown in Fig. 2, it can be seen that L- (+)-wine
Stone acid-complex film modified electrode of graphene quantum dot has preferable recognition effect, I to D-trpD/ILIt is 2.47.
Embodiment two：
It includes following steps to prepare D- (-)-complex film modified electrode of tartaric acid-graphene quantum dot：
(1) 2.0g citric acids are weighed to be added in 10mL crucibles, heat reaction under the conditions of 200 DEG C in program-controlled cabinet-type electric furnace
30min takes out solid sample, with ultra-pure water ultrasonic dissolution after reaction.After product fully dissolves, filter yellow is saturating
Bright solution, is kept in dark place at normal temperatures.
(2) it is working electrode that experiment, which uses three-electrode system, glass-carbon electrode (diameter 3mm), and platinum plate electrode is to satisfy to electrode
It is reference electrode with calomel electrode (SCE), is dipped in graphene quantum dot solution, in the electrification of 0V~1.0V (vs.SCE)
It learns in window ranges, cyclic voltammetric 50 is swept with the speed of 0.1V/s and is enclosed, modified by graphene quantum dot electrode is obtained.Again by the modification
Electrode immerses in 0.1g/mL D- (-)-tartaric acid solution prepared, with the potential deposition 400s of potentiostatic method application+1.0V, system
It is standby to obtain D- (-)-tartaric acid-complex film modified electrode of graphene quantum dot.
D- (-)-tartaric acid of preparation-complex film modified electrode of graphene quantum dot is immersed into a concentration of of configuration respectively
In 0.5mmol/L L-/D- tryptophan solutions, the recognition effect figure of Tryptophan enantiomer is shown in Fig. 3, it can be seen that D- (-)-wine
Stone acid-complex film modified electrode of graphene quantum dot is more preferable to the recognition effect of L-Trp, IL/IDIt is 2.71.
Comparative example one:
Modified by graphene quantum dot electrode is prepared, preparation process is as follows：
(1) 2.0g citric acids are weighed to be added in 10mL crucibles, heat reaction under the conditions of 200 DEG C in program-controlled cabinet-type electric furnace
30min takes out solid sample, with ultra-pure water ultrasonic dissolution after reaction.After product fully dissolves, filter yellow is saturating
Bright solution, is kept in dark place at normal temperatures.
(2) it is working electrode that experiment, which uses three-electrode system, glass-carbon electrode (diameter 3mm), and platinum plate electrode is to satisfy to electrode
It is reference electrode with calomel electrode (SCE), is dipped in graphene quantum dot solution, in the electrification of 0V~1.0V (vs.SCE)
It learns in window ranges, cyclic voltammetric 50 is swept with the speed of 0.1V/s and is enclosed, modified by graphene quantum dot electrode is obtained.
The modified by graphene quantum dot electrode of preparation is used for the identification of Tryptophan enantiomer, which is immersed respectively
In a concentration of 0.5mmol/L L-/D- tryptophan solutions of configuration, as shown in figure 4, IL/IDIt is 1.03.
Comparative example two：
L- (+)-tartaric acid modified electrode is prepared, preparation process is as follows：
It is working electrode that experiment, which uses three-electrode system, glass-carbon electrode (diameter 3mm), and platinum plate electrode is to electrode, saturation
Calomel electrode (SCE) is reference electrode, is dipped in prepared 0.1g/mL L- (+)-tartaric acid solution, uses potentiostatic method
L- (+)-tartaric acid modified electrode is prepared in the potential deposition 400s of application+1.0V.
The L- (+) of preparation-tartaric acid modified electrode is used for the identification of Tryptophan enantiomer, which is immersed respectively
In a concentration of 0.5mmol/L L-/D- tryptophan solutions of configuration, as shown in figure 5, ID/ILIt is 1.34.
Comparative example three：
D- (-)-tartaric acid modified electrode is prepared, preparation process is as follows：
It is working electrode that experiment, which uses three-electrode system, glass-carbon electrode (diameter 3mm), and platinum plate electrode is to electrode, saturation
Calomel electrode (SCE) is reference electrode, is dipped in prepared 0.1g/mL D- (-)-tartaric acid solution, uses potentiostatic method
D- (-)-tartaric acid modified electrode is prepared in the potential deposition 400s of application+1.0V.
The D- (-) of preparation-tartaric acid modified electrode is used for the identification of Tryptophan enantiomer, which is immersed respectively
In a concentration of 0.5mmol/L L-/D- tryptophan solutions of configuration, as shown in fig. 6, IL/IDIt is 1.28.
The present invention make full use of tartaric acid have chiral environment (be respectively L- (+)-tartaric acid and D- (-)-tartaric acid) this
One characteristic prepares the excellent complex film modified electrode of tartaric acid-graphene quantum dot of recognition performance in conjunction with graphene quantum dot.It should
Complex film modified electrode possesses more excellent recognition effect than single tartaric acid or modified by graphene quantum dot electrode.
Claims (4)
1. a kind of preparation method of the complex film modified electrode of tartaric acid-graphene quantum dot of Tryptophan enantiomer for identification,
It is characterized in that：Steps are as follows：
A, graphene quantum dot is prepared：Citric acid is added in crucible, is heated in program-controlled cabinet-type electric furnace, after reaction
Solid sample is taken out, yellow transparent solution is filtered to obtain after product is fully dissolved with ultra-pure water ultrasonic dissolution, kept away at normal temperatures
Light preserves；
B, the complex film modified electrode of tartaric acid-graphene quantum dot is prepared：Experiment uses three-electrode system, and glass-carbon electrode is work
Electrode, platinum plate electrode are to electrode, and saturated calomel electrode is reference electrode, and it is molten which is immersed graphene quantum dot
In liquid, within the scope of the electrochemical window of 0V~1.0V, carries out cyclic voltammetry scan and obtain modified by graphene quantum dot electrode, then will
The modified electrode immerses in prepared L- (+)-tartaric acid or D- (-)-tartaric acid solution, and potentiostatic method is prepared into tartaric acid-
The complex film modified electrode of graphene quantum dot；
C, electrochemical process identifies Tryptophan enantiomer：Experiment use three-electrode system, L- (+)-tartaric acid-graphene quantum dot or
D- (-)-tartaric acid-complex film modified electrode of graphene quantum dot is working electrode, and platinum plate electrode is to electrode, saturation calomel electricity
Extremely reference electrode immerses the three-electrode system in prepared L-/D- tryptophan solutions respectively, and difference arteries and veins is carried out after standing
Punching scanning.
2. a kind of tartaric acid of Tryptophan enantiomer for identification-graphene quantum dot composite membrane is repaiied according to claim 1
The preparation method of electrode is adornd, it is characterized in that：Citric acid quality is 0.1~15.0g in the step a, and reaction temperature is 50~400
DEG C, the reaction time is 0.1~3h.
3. a kind of tartaric acid of Tryptophan enantiomer for identification-graphene quantum dot composite membrane is repaiied according to claim 1
The preparation method of electrode is adornd, it is characterized in that：It is 4 that glass-carbon electrode scans the number of turns in graphene quantum dot solution in the step b
~100 circles prepare the tartaric acid solution of 0.1g/mL, and sedimentation potential is+1.0V, and electrodeposition time is 10~1000s.
4. a kind of tartaric acid of Tryptophan enantiomer for identification-graphene quantum dot composite membrane is repaiied according to claim 1
The preparation method of electrode is adornd, it is characterized in that：L- (+)-tartaric acid-graphene quantum dot or D- (-)-tartaric acid-graphene quantum
The complex film modified electrode of point immerses a concentration of 0.1~10mmol/L of L-/D- tryptophan solutions respectively.
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| CN106928434B (en) * | 2017-03-07 | 2019-02-15 | 常州大学 | A kind of method and its application by adulterating tartrate ion synthesis of chiral conducting polymer |
| CN108375617B (en) * | 2018-02-09 | 2020-05-19 | 华中科技大学 | Double-nanoenzyme modified carbon fiber composite material, and preparation method and application thereof |
| CN108445064B (en) * | 2018-03-05 | 2019-12-31 | 常州大学 | Preparation method of graphene quantum dot-bovine serum albumin composite material applicable to electrochemical chiral recognition |
| CN109908765B (en) * | 2019-03-14 | 2021-12-03 | 闽江学院 | L-tartaric acid modified self-made microporous polymer membrane and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1670012A (en) * | 2005-01-14 | 2005-09-21 | 清华大学 | Reagent for separating amino acid enantiomer |
| CN103193624A (en) * | 2013-03-22 | 2013-07-10 | 江苏大学 | Identification and separation method for tartaric acid enantiomer by chiral column material, and application of chiral column material |
| CN105044173A (en) * | 2015-07-06 | 2015-11-11 | 常州大学 | Production method of graphene quantum dot/beta-cyclodextrin composite membrane modified electrode, and application of electrode in electrochemical identification of tryptophan enantiomers. |
| CN105044178A (en) * | 2015-04-23 | 2015-11-11 | 常州大学 | Chiral recognition to tryptophan enantiomer containing zinc ion by chiral sensor based on chitosan/sodium alginate |
-
2016
- 2016-05-16 CN CN201610321006.8A patent/CN106018517B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1670012A (en) * | 2005-01-14 | 2005-09-21 | 清华大学 | Reagent for separating amino acid enantiomer |
| CN103193624A (en) * | 2013-03-22 | 2013-07-10 | 江苏大学 | Identification and separation method for tartaric acid enantiomer by chiral column material, and application of chiral column material |
| CN105044178A (en) * | 2015-04-23 | 2015-11-11 | 常州大学 | Chiral recognition to tryptophan enantiomer containing zinc ion by chiral sensor based on chitosan/sodium alginate |
| CN105044173A (en) * | 2015-07-06 | 2015-11-11 | 常州大学 | Production method of graphene quantum dot/beta-cyclodextrin composite membrane modified electrode, and application of electrode in electrochemical identification of tryptophan enantiomers. |
Non-Patent Citations (2)
| Title |
|---|
| Preparative Enantioseparation of dl-α-Methylbenzylamine by High-Speed Countercurrent Chromatography using l‐(+)‐Tartaric Acid as Chiral Selector;Y. Cai 等;《Journal of Liquid Chromatography & Related Technologies》;20070410;第30卷;第1489-1494页 * |
| Selective adsorption of L-TA D-TA by β-cyclodextrin derivative modified with l-tryptophan Isotherm, kinetic and thermodynamics studies;Kaichen He 等;《Journal of Industrial and Engineering Chemistry》;20130709;第20卷;第1293-1300页 * |
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