CN106011017B - method for realizing rapid proliferation of anaerobic ammonium oxidation bacteria - Google Patents
method for realizing rapid proliferation of anaerobic ammonium oxidation bacteria Download PDFInfo
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Abstract
The invention discloses a method for realizing rapid proliferation of anammox bacteria, which comprises the following steps: based on the maximum electron transfer capacity of anammox bacteria, the MBR reactor is combined to start anammox, mixed sludge of nitrified sludge (90%) and anaerobic granular sludge (10%) is inoculated, the sludge retention time is gradually shortened, the maximum specific growth rate of the anammox bacteria is improved, and the rapid proliferation of the anammox bacteria is realized. Aiming at the characteristics of long starting time of anaerobic ammonia oxidation and low sludge growth rate, the invention combines MBR and anaerobic ammonia oxidation together and inoculates an optimal sludge source, so that the novel high-efficiency denitrification technology can be applied to actual production in a large scale as soon as possible.
Description
Technical Field
the invention belongs to the technical field of sewage treatment, and particularly relates to a method for realizing rapid proliferation of anaerobic ammonium oxidation bacteria.
Background
With the development of industries such as petrifaction, food and pharmacy and the continuous improvement of the living standard of people, the content of nitrogen compounds in industrial wastewater and domestic sewage is increased rapidly, and the removal of nitrogen pollutants is one of the research hotspots in the field of sewage treatment. The traditional denitrification technology consists of two parts of nitrification and denitrification, and the traditional biological denitrification method has the advantages of good treatment effect, stable and reliable treatment process, convenient operation and management and no secondary pollution. But the nitration process requires a large amount of energy consumption; the denitrification process requires a certain amount of organic matter, which increases the operating cost.
Compared with the traditional biological denitrification process, anaerobic ammonia oxidation has the following unique advantages: (1) an organic carbon source is not required to be added in the reaction process as an electron donor, so that about 40% of operation cost is saved, and secondary pollution of CO2 is prevented; (2) half of NH4+ -N is only required to be oxidized into NO 2-N in the pre-reaction, so that the oxygen supply is saved; (3) the growth of the anaerobic ammonium oxidation bacteria is slow, the sludge yield is reduced by 90 percent, and the sludge treatment cost is saved. Although anammox has such many advantages, it cannot be quickly applied to engineering because of the long multiplication time of anammox bacteria and the need for strict anaerobic conditions. At present, many scholars at home and abroad try to explore ways for rapidly starting and enriching anammox bacteria with high denitrification efficiency by different methods, a plurality of referential experiences are accumulated, and research hotspots are mainly focused on: selecting the sludge inoculated in the anaerobic ammonia oxidation reactor, and selecting the reactor suitable for the growth characteristics of anaerobic ammonia oxidation bacteria.
MBR is used as a novel bioreactor, and has a very wide application prospect. Due to the efficient interception function of the membrane, microorganisms are completely intercepted in the bioreactor, and higher microorganism concentration can be maintained in the system, so that enrichment and culture of anaerobic ammonium oxidation bacteria are facilitated. Is an ideal reactor for starting Anammox.
At present, the anaerobic ammonia oxidation has the problems of long starting time and slow sludge growth rate, and the practical application of the anaerobic ammonia oxidation is limited to a great extent. In order to better promote the wide application of the anaerobic ammonia oxidation technology to the practical process, the effective and rapid enrichment of the anaerobic ammonia oxidation bacteria is particularly important.
The anaerobic ammonia oxidation process has the advantages of high-efficiency denitrification, low energy consumption, low sludge yield and the like, and is considered to be a technology with application prospect in the field of denitrification. However, the amammox technology is still engineered, and some technical problems which need to be solved urgently still exist. The problem of enrichment of Anammox bacteria is solved firstly. The growth of the Anramox bacteria serving as the main body of the Anramox technology is extremely slow, and the Anramox bacteria become the bottleneck of popularization and application of the technology.
disclosure of Invention
The invention aims to provide a method for realizing rapid proliferation of Anammox bacteria, and aims to solve the problems of long Anammox starting time, slow sludge growth rate and extremely slow Anammox bacteria enrichment and growth in the traditional denitrification technology.
The invention is realized by the method for realizing the rapid proliferation of the anaerobic ammonium oxidation bacteria, which comprises the following steps:
A method for realizing rapid proliferation of anaerobic ammonium oxidation bacteria comprises the following steps:
And inoculating mixed sludge of nitrifying sludge and anaerobic granular sludge in the MBR, and gradually shortening the retention time of the sludge to improve the maximum specific growth rate of the anaerobic ammonium oxidation bacteria so as to realize the rapid proliferation of the anaerobic ammonium oxidation bacteria.
Further, the method for realizing rapid proliferation of the anaerobic ammonium oxidation bacteria specifically comprises the following steps:
inoculating mixed sludge of nitrifying sludge and anaerobic granular sludge into the MBR, and starting anaerobic ammonia oxidation;
in the starting process, gradually shortening the sludge retention time from 15 days to 3 days, and measuring the maximum specific growth rate of the anaerobic ammonium oxidation bacteria;
When the sludge retention time is 3 days, the maximum specific growth rate of the anammox bacteria is measured, and the rapid proliferation of the anammox bacteria is realized.
Furthermore, the rapid proliferation analysis of the anammox bacteria is required before the rapid proliferation method of the anammox bacteria is realized, and the analysis comprises the analysis based on the maximum electron transfer capacity of the anammox bacteria.
further, the nitrified sludge was 90% and the anaerobic granular sludge was 10%.
further, the starting anaerobic ammonia oxidation method comprises the following steps:
Inoculating mixed sludge of nitrifying sludge and anaerobic granular sludge in the MBR, culturing by adopting simulated water distribution, controlling the pH of inlet water to be 7.5 +/-0.5, and finishing starting anaerobic ammonia oxidation after 80 days.
further, the sludge retention time is gradually shortened from 15 days to 3 days, and the method for determining the maximum specific growth rate of the anaerobic ammonium oxidation bacteria comprises the following steps: controlling the hydraulic retention time to be 1 day, controlling the sludge retention time to be 15 days, continuously culturing for 60 days, measuring that the maximum specific growth rate of the anammox bacteria is 0.067d-1 at the moment, gradually shortening to 3 days, measuring that the maximum specific growth rate of the anammox bacteria is 0.33d-1, and increasing the proliferation rate of the anammox bacteria to 3 times of the original proliferation rate.
Furthermore, a rapid proliferation device for realizing anaerobic ammonium oxidation bacteria is provided with a water inlet cylinder, a peristaltic pump, a reactor, a membrane device, a stirrer and a water outlet cylinder; the peristaltic pump is provided with a first peristaltic pump and a second peristaltic pump; the inlet of the first peristaltic pump is connected with the water inlet cylinder, the outlet of the first peristaltic pump is connected with the inlet of the reactor, the outlet of the reactor is connected with the inlet of the second peristaltic pump, the outlet of the second peristaltic pump is connected with the water outlet cylinder, the membrane device is installed in the reactor, and the stirring machine is inserted in the membrane device.
according to the invention, the anaerobic ammonia oxidation is started by MBR, the mixed sludge of nitrified sludge (90%) and anaerobic granular sludge (10%) is inoculated, the sludge retention time is gradually shortened, the maximum specific growth rate of anaerobic ammonia oxidation bacteria is increased, the maximum proliferation rate of anaerobic ammonia oxidation bacteria is 0.33d-1, and is increased by 2 times compared with the proliferation rate of anaerobic ammonia oxidation bacteria (less than 0.1d-1) reported by most of literatures, so that the aim of rapidly proliferating the anaerobic ammonia oxidation bacteria is fulfilled;
The method for realizing rapid proliferation of the Anammox bacteria, provided by the invention, enables the reactor to enrich more Anammox bacteria in a shorter time, and solves the problems of long starting time of Anammox, slow sludge growth rate and extremely slow enrichment and growth of Anammox bacteria in the traditional denitrification technology.
Table 1 is a summary of the anaerobic ammonium oxidation bacteria kinetic parameters of the present invention;
TABLE 1
Drawings
FIG. 1 is a flow chart of a method for achieving rapid proliferation of anammox bacteria according to an embodiment of the present invention.
FIG. 2 shows a rapid proliferation apparatus for anammox bacteria according to the present invention.
In the figure: 1. a water inlet cylinder; 2. a first peristaltic pump; 3. a membrane device; 4. a blender; 5. a water outlet barrel; 6. a reactor; 7. a second peristaltic pump.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
The application of the principles of the present invention will be further described with reference to the accompanying drawings and specific embodiments.
as shown in fig. 1:
a method for realizing rapid proliferation of anaerobic ammonium oxidation bacteria comprises the following steps:
And inoculating mixed sludge of nitrifying sludge and anaerobic granular sludge in the MBR, and gradually shortening the retention time of the sludge to improve the maximum specific growth rate of the anaerobic ammonium oxidation bacteria so as to realize the rapid proliferation of the anaerobic ammonium oxidation bacteria.
as shown in fig. 1: the method for realizing the rapid proliferation of the anaerobic ammonium oxidation bacteria comprises the following steps:
S101: carrying out rapid proliferation analysis on anaerobic ammonium oxidation bacteria;
S102: inoculating mixed sludge of nitrifying sludge and anaerobic granular sludge into the MBR, and starting anaerobic ammonia oxidation;
s103: in the starting process, gradually shortening the sludge retention time from 15 days to 3 days, and measuring the maximum specific growth rate of the anaerobic ammonium oxidation bacteria;
S104: when the sludge retention time is 3 days, the maximum specific growth rate of the anammox bacteria is measured, and the rapid proliferation of the anammox bacteria is realized.
the nitrified sludge accounts for 90 percent, and the anaerobic granular sludge accounts for 10 percent.
The analysis described in the anammox rapid proliferation assay includes an assay based on the maximum electron transfer capacity of anammox bacteria.
the starting anaerobic ammonia oxidation method comprises the following steps:
Inoculating mixed sludge of nitrifying sludge and anaerobic granular sludge in the MBR, culturing by adopting simulated water distribution, controlling the pH of inlet water to be 7.5 +/-0.5, and finishing starting anaerobic ammonia oxidation after 80 days.
The sludge retention time is gradually shortened from 15 days to 3 days, and the method for determining the maximum specific growth rate of the anaerobic ammonium oxidation bacteria comprises the following steps: controlling the hydraulic retention time to be 1 day, controlling the sludge retention time to be 15 days, continuously culturing for 60 days, measuring that the maximum specific growth rate of the anammox bacteria is 0.067d-1 at the moment, gradually shortening to 3 days, measuring that the maximum specific growth rate of the anammox bacteria is 0.33d-1, and increasing the proliferation rate of the anammox bacteria to 5 times of the original proliferation rate.
Starting anaerobic ammonia oxidation by combining MBR, gradually shortening sludge retention time by inoculating mixed sludge of nitrified sludge (90%) and anaerobic granular sludge (10%), improving the maximum specific growth rate of anaerobic ammonia oxidizing bacteria, and realizing rapid proliferation of anaerobic ammonia oxidizing bacteria.
As shown in fig. 2 |: a rapid proliferation device for realizing anaerobic ammonium oxidation bacteria is provided with a water inlet cylinder 1, a peristaltic pump, a reactor 6, a membrane device 3, a stirrer 4 and a water outlet cylinder 5; the peristaltic pump is provided with a first peristaltic pump 2 and a second peristaltic pump 7; the inlet of the first peristaltic pump is connected with the water inlet cylinder, the outlet of the first peristaltic pump is connected with the inlet of the reactor, the outlet of the reactor is connected with the inlet of the second peristaltic pump, the outlet of the second peristaltic pump is connected with the water outlet cylinder, the membrane device is installed in the reactor, and the stirring machine is inserted in the membrane device.
According to the invention, the MBR is adopted to start the anaerobic ammonia oxidation, the maximum specific growth rate of the anaerobic ammonia oxidation bacteria is improved by shortening the sludge retention time, and the optimal working condition in the starting process of the Anammox membrane bioreactor is inspected, so that the aim of rapidly proliferating the anaerobic ammonia oxidation bacteria is fulfilled.
Aiming at the characteristics of long starting time of anaerobic ammonia oxidation and low sludge growth rate, the invention combines MBR and anaerobic ammonia oxidation together, inoculates the optimal sludge source, and tests and optimizes the process operation conditions and process operation parameters of the rapid proliferation anaerobic ammonia oxidation bacteria. So that the novel high-efficiency denitrification technology can be applied to the actual production in a large scale as soon as possible.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (6)
1. a method for realizing rapid proliferation of anammox bacteria, comprising:
Based on the maximum electron transfer capacity of the anammox bacteria, mixed sludge of nitrified sludge and anaerobic granular sludge is inoculated in an MBR (membrane bioreactor), and the rapid proliferation of the anammox bacteria is realized by gradually shortening the retention time of the sludge and increasing the maximum specific growth rate of the anammox bacteria;
The method for realizing the rapid proliferation of the anaerobic ammonium oxidation bacteria comprises the following steps:
inoculating mixed sludge of nitrifying sludge and anaerobic granular sludge into the MBR, and starting anaerobic ammonia oxidation;
In the starting process, gradually shortening the sludge retention time from 15 days to 3 days, and measuring the maximum specific growth rate of the anaerobic ammonium oxidation bacteria;
When the sludge retention time is 3 days, the maximum specific growth rate of the anammox bacteria is measured, and the rapid proliferation of the anammox bacteria is realized.
2. the method for rapid proliferation of anammox bacteria according to claim 1, wherein the rapid proliferation of anammox bacteria is further analyzed by anammox bacteria rapid proliferation analysis, and the analysis comprises analysis based on maximum electron transfer capacity of anammox bacteria.
3. The method for achieving rapid proliferation of anammox bacteria according to claim 1, wherein the nitrified sludge is 90% and the anaerobic granular sludge is 10%.
4. The method for achieving rapid proliferation of anammox bacteria according to claim 1, wherein the starting of the anammox method comprises:
inoculating mixed sludge of nitrifying sludge and anaerobic granular sludge in the MBR, culturing by adopting simulated water distribution, controlling the pH of inlet water to be 7.5 +/-0.5, and finishing starting anaerobic ammonia oxidation after 80 days.
5. The method for achieving rapid proliferation of anammox bacteria according to claim 1,
The sludge retention time is gradually shortened from 15 days to 3 days, and the method for determining the maximum specific growth rate of the anaerobic ammonium oxidation bacteria comprises the following steps: controlling the hydraulic retention time to be 1 day and the sludge retention time to be 15 days, continuously culturing for 60 days, measuring the maximum specific growth rate of the anammox bacteria at the moment, and measuring the maximum specific growth rate of the anammox bacteria when the maximum specific growth rate is gradually shortened to 3 days.
6. The rapid propagation apparatus for rapid propagation of anammox bacteria according to claim 1, wherein the apparatus for rapid propagation of anammox bacteria comprises a water inlet tube, a peristaltic pump, a reactor, a membrane unit, a stirrer, and a water outlet tube; the peristaltic pump is provided with a first peristaltic pump and a second peristaltic pump; the inlet of the first peristaltic pump is connected with the water inlet cylinder, the outlet of the first peristaltic pump is connected with the inlet of the reactor, the outlet of the reactor is connected with the inlet of the second peristaltic pump, the outlet of the second peristaltic pump is connected with the water outlet cylinder, the membrane device is installed in the reactor, and the stirring machine is inserted in the membrane device.
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