CN106010960B - With the culture dish for microorganism detection referring to function - Google Patents
With the culture dish for microorganism detection referring to function Download PDFInfo
- Publication number
- CN106010960B CN106010960B CN201610570850.4A CN201610570850A CN106010960B CN 106010960 B CN106010960 B CN 106010960B CN 201610570850 A CN201610570850 A CN 201610570850A CN 106010960 B CN106010960 B CN 106010960B
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- CN
- China
- Prior art keywords
- bracket
- wire
- support base
- upper cover
- quartz vibrator
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
Abstract
The invention discloses a kind of culture dishes for microorganism detection with referring to function, comprising: pedestal, pedestal are recessed downwards by upper surface and form a cavity；Support base, support base are contained in cavity, and support base upper surface is equipped with a circular trough；Crystal oscillator device, crystal oscillator device includes bracket, quartz vibrator, the rounded structure of bracket, quartz vibrator is embedded on bracket, the both ends of bracket further respectively have wire restraint structure, quartz vibrator is laid with foil-like excitation electrode in tow sides, and electrode and external circuit junction is motivated to be set as disc-shape；Electric wire, electric wire include conducting wire and the plate-like clamp structure for being connected to wire end, and plate-like clamp structure is clamped on disc-shape and is electrically connected, and conducting wire passes through from wire restraint structure；Upper cover, upper cover are hermetically covered on pedestal, and upper cover is equipped with the interface for entering for culture solution sample solution and being discharged.The present invention can shorten incubation time, improve detection accuracy.
Description
Technical field
The present invention relates to technical field of microbial detection more particularly to one kind can shorten incubation time, and it is quasi- to improve detection
The microbial cultivation device of true property.
Background technique
With the development of economy, continuous improvement of people's living standards also proposes the consciousness of food safety constantly
It is high.In the past, as carry out in food or beverage whether the method for the judgement containing spoilage organisms, including use immune measurement method,
The machines such as ELISA method, gas-chromatography mass analysis, liquid chromatographic mass analytic approach survey method, but the pre-treatment measured is many and diverse, sentences
Fixed precision can not be said sufficiently.To usually take a long time to cultivate spoilage organisms.
Using the technology for including label microorganism, to protrude between the light of microorganism sending and the light of growth support sending
Comparison.Due to using the optical system to feature (such as wavelength or intensity) sensitivity of the light of sending, so using specific
Fluorescent marker, fluorogen or use can be exposed by the enzyme of such as ATP bioluminescence issued, can carry out getting up early to microorganism
Detection.
In addition, being cultivated by agar medium is most common and most ancient one of method, wherein culture medium is for one
Kind or several microorganism types are more or less selectable.In a period of can usually extend to several days, the culture medium is suitable
It is cultivated at a temperature of, for growing the microorganism to be found.The shortcomings that this cultural method is still to be formed on agar
Bacterium colony be able to carry out the visible detection of naked eye, need relatively long cultivation cycle.It will also realize that from current technological level, it can be with
Chain reaction (also referred to as PCR breeding) is executed by the breeding of DNA sequence dna or RNA sequence, so that it is determined that there are specific in sample
Microorganism.The shortcomings that these methods, is: in order to make the bacterium colony formed on agar medium be able to carry out the visible detection of naked eye, needing
Want relatively long cultivation cycle.And will also realize that from current technological level, the breeding of NDA sequence or RNA sequence can be passed through
Chain polymerization reaction (also referred to as PCR breeding) is executed, so that it is determined that the specified microorganisms in sample.The shortcomings that these methods is desirable
Several DNA chain, i.e., several microorganism, at least tens kinds of microorganisms, this method of generally requiring are not based on the side of growth
Method is sensitive.
Agar medium is relatively common come the method for cultivating the microorganism to be detected, but its shortcoming is culture
Overlong time, how to shorten incubation time, improve efficiency to become and need the bottleneck broken through.
Summary of the invention
The object of the present invention is to provide one kind can shorten incubation time, improves the microculture dress of detection accuracy
It sets.
To achieve the goals above, technical solution provided by the invention are as follows: provide it is a kind of with referring to function for micro-
The culture dish of biological detection, comprising:
Pedestal, the pedestal are recessed downwards by upper surface and form a cavity；
Support base, the support base are contained in the cavity, and the support base upper surface is equipped with a circular trough；
Crystal oscillator device, the crystal oscillator device is fixed in the circular trough, including bracket, quartz vibrator, and the bracket is in
Circular configuration, the quartz vibrator are embedded on the bracket, and the both ends of the bracket further respectively have wire restraint structure, institute
State quartz vibrator and be set as the first vibration area and the second vibration area, first vibration area and the second vibration area it is positive and negative
Two sides is laid with foil-like excitation electrode, and the excitation electrode and external circuit junction are set as disc-shape, is located at described
Antibody layer is formed on the excitation electrode of first vibration area, on the excitation electrode on second vibration area
Form barrier layer；
Electric wire, the electric wire include conducting wire and the plate-like clamp structure for being connected to the wire end, described
Plate-like clamp structure is clamped on the disc-shape and is electrically connected, and the conducting wire is logical from the wire restraint structure
It crosses；
Upper cover, the upper cover are hermetically covered on the pedestal, and the upper cover is equipped with to be entered for culture solution sample solution
And the interface of discharge.
The conductive hole for drawing the conductor wire is equipped with through the lower surface of the upper cover and side wall.
It further include sealing ring, the sealing ring is arranged in the top edge that the lower surface of the upper cover corresponds to the support base.
It further include temperature control equipment, the temperature control equipment is set to the cavity bottom, and the support base is thermally conductive
Material of good performance is made, and controls between the temperature control equipment across temperature of the support base to the quartz vibrator
System.
The bracket includes annular body structure, and the annular body structure is convexly equipped with flange, the stone to center of circle direction
English oscillator is carried on the flange.
The outer rim of the flange is connected with reticular structure, and the quartz vibrator is carried on the flange and the reticular structure
On.
The wire restraint structure be set on the bracket both ends, and it is described through allowing for bracket upper and lower surface setting
The through-hole that conducting wire passes through.
The wire restraint structure be set on the bracket both ends notch, the wire restraint in the notch with it is described
In the space in round groove sidewall besieged city.
It further include crystal oscillator device fixed structure, the crystal oscillator device fixed structure includes determining set on the circular trough bottom
Position column and the location hole cooperated with the positioning column.
Compared with prior art, due to having in the culture dish for microorganism detection referring to function in the present invention, institute
Stating crystal oscillator device includes bracket, quartz vibrator, the rounded structure of bracket, and the quartz vibrator is embedded on the bracket,
The both ends of the bracket further respectively have wire restraint structure, and the quartz vibrator is laid with foil-like excitation electricity in tow sides
Pole, and the excitation electrode and external circuit junction are set as disc-shape.Therefore, the quartz vibrator in the present invention can
It is preferably fixed, the quartz vibrator can be prevented during the work time, appearance connect unstable with the electric wire
Situation is furthermore possible to improve quartz vibrator holding balance in vibration, helps to improve the quartz vibrator vibration frequency
The accuracy of measurement.
Through the following description and in conjunction with the attached drawings, the present invention will become more fully apparent, these attached drawings are used to explain the present invention
Embodiment.
Detailed description of the invention
Fig. 1 is the schematic diagram of the one embodiment for the culture dish for microorganism detection that the present invention has reference function.
Fig. 2 is the explosive view with the culture dish for microorganism detection referring to function as shown in Figure 1.
Fig. 3 is the structure of the quartz vibrator with the culture dish for microorganism detection referring to function as shown in Figure 1
Schematic diagram.
Fig. 4 is an implementation of the bracket with the culture dish for microorganism detection referring to function as shown in Figure 1
The schematic diagram of example.
Fig. 5 is another reality of the bracket with the culture dish for microorganism detection referring to function as shown in Figure 1
Apply the schematic diagram of example.
Fig. 6 is the crystal oscillator device with the culture dish for microorganism detection referring to function and support as shown in Figure 1
The schematic diagram of seat cooperation.
Specific embodiment
The embodiment of the present invention described referring now to the drawings, similar element numbers represent similar element in attached drawing.Such as
It is upper described, as shown in Figure 1, 2, 3, the culture dish for microorganism detection provided in an embodiment of the present invention having referring to function
100, comprising:
Pedestal 1, the pedestal 1 are recessed downwards by upper surface and form a cavity 10；
Support base 2, the support base 2 are contained in the cavity 10, and 2 upper surface of the support base is equipped with a circular trough
20；
Crystal oscillator device 3, the crystal oscillator device are fixed in the circular trough 20, including bracket 30, quartz vibrator 31, described
The rounded structure of bracket 30, the quartz vibrator 31 are embedded on the bracket 30, and the both ends of the bracket 30 further respectively have
Wire restraint structure 32, the quartz vibrator 31 are set as the first vibration area 310 and the second vibration area 311, first vibration
The tow sides of dynamic region 310 and the second vibration area 311 are laid with foil-like excitation electrode 33, and the excitation electrode 33 and
External circuit junction is set as disc-shape 34, which is to be able to improve contact area, is located at described the
On the excitation electrode 33 of one vibration area 310 form antibody layer 312, on second vibration area 311 described in
It motivates and forms barrier layer 313 on electrode 33；The side that the barrier layer 313 is arranged does not have the absorption of microorganism, therefore is testing
The variation of the vibration frequency of second vibration area 311 is the vibration frequency variation of first vibration area 310 in the process
Reference, the variation of the vibration frequency of second vibration area 311 should tend towards stability, and its variation should also be
Variation is generated due to temperature factor.Therefore in test process, by first vibration area 310 and the second vibration area 311
Vibration frequency difference absolute value, can offset with environmental change generate frequency variation, can take out only with micro- life
The variation of the frequency of oscillation for being adsorbed as cause of object.
Electric wire 4, the electric wire 4 include conducting wire 40 and the plate-like clamp structure for being connected to 40 end of conducting wire
41, the plate-like clamp structure 41 is clamped on the disc-shape 34 and is electrically connected, and the conducting wire 40 is led from described
Line position limiting structure 32 passes through；
Upper cover 5, the upper cover 5 are hermetically covered on the pedestal 1, the upper cover 5 be equipped with for culture solution sample solution
Into and discharge interface 50.
The conductive hole 51 for drawing the conducting wire 40 is equipped with through the lower surface of the upper cover 5 and side wall.
As shown in Fig. 2, further including sealing ring 6, the lower surface of the upper cover 5 corresponds to the top edge setting of the support base 2
The sealing ring 6.When being covered on the upper cover 5 on the pedestal 1 by the sealing ring 6, a sealing space is collectively formed.
It further include temperature control equipment 7, the temperature control equipment 7 is set to 10 bottom of cavity, and the support base 2 is
The good material of heating conduction is made, across the support base 2 to the temperature of the quartz vibrator 31 between the temperature control equipment 7
Degree is controlled.
As shown in figure 4, the bracket 30 includes annular body structure 300, the annular body structure 300 is to center of circle direction
It is convexly equipped with flange 301, the quartz vibrator 31 is carried on the flange 301.
As shown in figure 4, the outer rim of the flange 301 is connected with reticular structure 302, the quartz vibrator 31 is carried on described
On flange 301 and the reticular structure 302.
As shown in figure 4, the wire restraint structure 32 be set on 30 both ends of bracket, and run through about 30 bracket
The through-hole for allowing the conducting wire 40 to pass through of surface setting.
As shown in figure 5, the wire restraint structure 32 is the notch set on the bracket both ends, the conducting wire 40 is limited in
In the space in the notch and 20 side wall of circular trough besieged city.
As shown in fig. 6, further including crystal oscillator device fixed structure 8, the crystal oscillator device fixed structure 8 includes being set to the circle
The positioning column 80 of 20 bottom of shape slot and the location hole 81 cooperated with the positioning column 80.
Compared with prior art, in conjunction with Fig. 1~6, due to having referring to function in the present invention for microorganism detection
Culture dish 100, the crystal oscillator device include bracket 30, quartz vibrator 31, the rounded structure of the bracket 30, the quartz vibrator
31 are embedded on the bracket, and the both ends of the bracket 30 further respectively have wire restraint structure 32, and the quartz vibrator 31 exists
Tow sides are laid with foil-like excitation electrode 33, and the excitation electrode 33 and external circuit junction are set as disc-shape
34, therefore, the quartz vibrator in the present invention can be preferably fixed, and the quartz vibrator can be prevented in the course of work
In, occur with the electric wire connecting unstable situation, is furthermore possible to improve the quartz vibrator and keeps putting down in vibration
Weighing apparatus helps to improve the accuracy of the quartz vibrator vibration frequency measurement.
The above disclosure is only a preferred embodiment of the invention, cannot limit the right of the present invention with this certainly
Range, therefore according to equivalent variations made by scope of the present invention patent, it is still within the scope of the present invention.
Claims (6)
1. a kind of culture dish for microorganism detection with referring to function characterized by comprising
Pedestal, pedestal are recessed downwards by upper surface and form a cavity；
Support base, the support base are contained in the cavity, and the support base upper surface is equipped with a circular trough；
Crystal oscillator device, the crystal oscillator device is fixed in the circular trough, including bracket, quartz vibrator, and the bracket is rounded
Structure, the quartz vibrator are embedded on the bracket, and the both ends of the bracket further respectively have wire restraint structure, the stone
English oscillator is set as the first vibration area and the second vibration area, the tow sides of first vibration area and the second vibration area
It is laid with foil-like excitation electrode, and the excitation electrode and external circuit junction are set as disc-shape, are located at described first
Antibody layer is formed on the excitation electrode of vibration area, is formed on the excitation electrode on second vibration area
Barrier layer；
Electric wire, the electric wire include conducting wire and the plate-like clamp structure for being connected to the wire end, the plate-like
Clamp structure is clamped on the disc-shape and is electrically connected, and the conducting wire passes through from the wire restraint structure；
Upper cover, the upper cover are hermetically covered on the pedestal, and the upper cover, which is equipped with, to enter and arranged for culture solution sample solution
Interface out；
The bracket includes annular body structure, and the annular body structure is convexly equipped with flange, the quartz vibration to center of circle direction
Son is carried on the flange；
The outer rim of the flange is connected with reticular structure, and the quartz vibrator is carried on the flange and the reticular structure；
It further include temperature control equipment, the temperature control equipment is set to the cavity bottom, and the support base is heating conduction
Good material is made, and controls between the temperature control equipment across temperature of the support base to the quartz vibrator.
2. the culture dish for microorganism detection as described in claim 1 having referring to function, which is characterized in that run through institute
The lower surface and side wall of stating upper cover are equipped with the conductive hole for drawing the conductor wire.
3. the culture dish for microorganism detection as described in claim 1 having referring to function, which is characterized in that further include
The sealing ring is arranged in sealing ring, the top edge that the lower surface of the upper cover corresponds to the support base.
4. the culture dish for microorganism detection as described in claim 1 having referring to function, which is characterized in that described to lead
Line position limiting structure be set on the bracket both ends, and through bracket upper and lower surface setting allow the conducting wire passes through it is logical
Hole.
5. the culture dish for microorganism detection as described in claim 1 having referring to function, which is characterized in that described to lead
Line position limiting structure is the notch set on the bracket both ends, and the wire restraint is encircled a city in the notch and the round groove sidewall
Space in.
6. the culture dish for microorganism detection as described in claim 1 having referring to function, which is characterized in that further include
Crystal oscillator device fixed structure, the crystal oscillator device fixed structure include set on the circular trough bottom positioning column and with it is described
The location hole of positioning column cooperation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610570850.4A CN106010960B (en) | 2016-07-19 | 2016-07-19 | With the culture dish for microorganism detection referring to function |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610570850.4A CN106010960B (en) | 2016-07-19 | 2016-07-19 | With the culture dish for microorganism detection referring to function |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106010960A CN106010960A (en) | 2016-10-12 |
| CN106010960B true CN106010960B (en) | 2019-08-09 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610570850.4A Active CN106010960B (en) | 2016-07-19 | 2016-07-19 | With the culture dish for microorganism detection referring to function |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106918582A (en) * | 2017-03-31 | 2017-07-04 | 华测检测认证集团股份有限公司 | Microbial detection device |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09145583A (en) * | 1995-11-14 | 1997-06-06 | Dev Center For Biotechnol | Piezoelectric sensor chip of cartridge-form |
| JP2006029873A (en) * | 2004-07-13 | 2006-02-02 | Nippon Dempa Kogyo Co Ltd | Quartz sensor and sensing device |
| CN101080625A (en) * | 2004-12-15 | 2007-11-28 | 日本电波工业株式会社 | Component measuring instrument |
| CN101084427A (en) * | 2004-12-15 | 2007-12-05 | 日本电波工业株式会社 | Crystal sensor and sensing device |
| CN102192860A (en) * | 2010-03-10 | 2011-09-21 | 日本电波工业株式会社 | Method of detecting microorganisms and microorganism detecting apparatus |
| CN205990402U (en) * | 2016-07-19 | 2017-03-01 | 华测检测认证集团股份有限公司 | There is the culture dish for microorganism detection of reference function |
-
2016
- 2016-07-19 CN CN201610570850.4A patent/CN106010960B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09145583A (en) * | 1995-11-14 | 1997-06-06 | Dev Center For Biotechnol | Piezoelectric sensor chip of cartridge-form |
| JP2006029873A (en) * | 2004-07-13 | 2006-02-02 | Nippon Dempa Kogyo Co Ltd | Quartz sensor and sensing device |
| CN101080625A (en) * | 2004-12-15 | 2007-11-28 | 日本电波工业株式会社 | Component measuring instrument |
| CN101084427A (en) * | 2004-12-15 | 2007-12-05 | 日本电波工业株式会社 | Crystal sensor and sensing device |
| CN102192860A (en) * | 2010-03-10 | 2011-09-21 | 日本电波工业株式会社 | Method of detecting microorganisms and microorganism detecting apparatus |
| CN205990402U (en) * | 2016-07-19 | 2017-03-01 | 华测检测认证集团股份有限公司 | There is the culture dish for microorganism detection of reference function |
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| Publication number | Publication date |
|---|---|
| CN106010960A (en) | 2016-10-12 |
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Effective date of registration: 20210309 Address after: 518101 Hua Cheng Building, No. 4, Liuxian Third Road, Xin'an Street, Baoan District, Shenzhen City, Guangdong Province Patentee after: SHENZHEN CTI LABORATORY Co.,Ltd. Address before: 518101 C building, Hongwei Industrial Park, 70 District, Guangdong, Shenzhen, Baoan District Patentee before: CENTRE TESTING INTERNATIONAL GROUP Co.,Ltd. |