CN106010960A - Microbiological detection culture dish with reference functions - Google Patents
Microbiological detection culture dish with reference functions Download PDFInfo
- Publication number
- CN106010960A CN106010960A CN201610570850.4A CN201610570850A CN106010960A CN 106010960 A CN106010960 A CN 106010960A CN 201610570850 A CN201610570850 A CN 201610570850A CN 106010960 A CN106010960 A CN 106010960A
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- China
- Prior art keywords
- culture dish
- support
- wire
- reference function
- microorganism detection
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Analytical Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a microbiological detection culture dish with reference functions. The microbiological detection culture dish comprises a base, a support seat, a crystal oscillation device, conductive wires and an upper cover. The upper surface of the base is upwardly sunken to form a cavity; the support seat is accommodated in the cavity, and a circular groove is formed in the upper surface of the support seat; the crystal oscillation device comprises a support and a quartz oscillator, the support is of a circular structure, the quartz oscillator is arranged on the support in an embedded manner, wire limiting structures are further respectively arranged at two ends of the support, foil-shaped excitation electrodes are uniformly distributed on the front surface and the rear surface of the quartz oscillator, and joints of the excitation electrodes and external circuits are in the shapes of discs; the conductive wires comprise wires and disc-shaped clamping structures, the disc-shaped clamping structures are connected to the tail ends of the wires and are clamped on the joints in the shapes of the discs to be electrically connected with the joints, and the wires penetrate the wire limiting structures; the upper cover hermetically covers the base and is provided with ports, and culture solution\test specimen solution can flow into the ports or can be drained from the ports. The microbiological detection culture dish has the advantages that the culture time can be shortened, and the detection accuracy can be improved.
Description
Technical field
The present invention relates to technical field of microbial detection, particularly relate to one and can shorten incubation time, improve
The microbial cultivation device of detection accuracy.
Background technology
Along with expanding economy, improving constantly of people's living standard, for the consciousness of food safety also not
Improve disconnectedly.In the past, as carrying out the method that whether contains the judgement of putrefaction bacteria in Foods or drinks, bag
Include use immunity measurement method, ELISA method, gas-chromatography mass analysis, liquid chromatographic mass analytic process etc.
Machine survey method, but measure pre-treatment numerous and diverse, it is determined that precision can not say fully.Thus, generally to spend
Expense cultivates putrefaction bacteria for a long time.
The technology including labelling microorganism, the light sent with prominent microorganism and growth holder is used to send
Contrast between light.Owing to using the optical system of the sensitivity of the feature (such as wavelength or intensity) to the light sent
System, so using specific fluorescent labeling, fluorogen or use can expose the biology sent by such as ATP
The enzyme of fluorescence, can carry out getting up early detection to microorganism.
Additionally, being cultivated by agar culture medium is one of most common and the most ancient method, wherein culture medium
It is more or less selectable for one or more microorganism type.In the period that generally can extend to several days
In, this culture medium is cultivated at a proper temperature, for the microorganism that growth is to be found.This cultivation
The shortcoming of method remains the bacterium colony formed on agar can carry out the visible detection of bore hole, needs relatively long
Cultivation cycle.Will also realize that from current technological level, DNA sequence or the breeding of RNA sequence can be passed through
Perform chain reaction (also referred to as PCR breeding), so that it is determined that sample exists specified microorganisms.These methods
Shortcoming be: in order to enable on agar culture medium formed bacterium colony carry out the visible detection of bore hole, need phase
To long cultivation cycle.And will also realize that from current technological level, NDA sequence or RNA can be passed through
The breeding of sequence performs chain polymerization reaction (also referred to as PCR breeding), so that it is determined that the specified microorganisms in sample.
The shortcoming of these methods is to need several DNA, the most several microorganism, it is generally required at least tens kinds
Microorganism, the method that this method is not based on growth is sensitive.
It is relatively common that agar culture medium cultivates the method for microorganism to be detected, but its weak point
Be cultivate overlong time, how can shorten incubation time, improve efficiency become need break through bottleneck.
Summary of the invention
It is an object of the invention to provide one and can shorten incubation time, improve the microorganism training of detection accuracy
Support device.
To achieve these goals, the technical scheme that the present invention provides is: provides a kind of and has reference function
For the culture dish of microorganism detection, including:
Base, described base is formed a cavity by upper surface concave downward;
Supporting seat, described support seat is contained in described cavity, and described support seat upper surface is provided with a circle
Groove;
Crystal oscillator device, described crystal oscillator device is fixed in described circular trough, including support, quartz vibrator, institute
Stating the rounded structure of support, described quartz vibrator is embedded on described support, and the two ends of described support are also distinguished
Being provided with wire restraint structure, described quartz vibrator is set as the first vibration area and the second vibration area, and described
The tow sides of one vibration area and the second vibration area are laid with foil-like exciting electrode, and described excitation electricity
Pole and external circuit junction are set as disc-shape, are positioned on the described exciting electrode of described first vibration area
Molding antibody layer, is positioned at molding barrier layer on the described exciting electrode on described second vibration area;
Electric wire, described electric wire includes wire and is connected to the plate-like clamp structure of described wire end,
Described plate-like clamp structure is clamped on described disc-shape and is electrically connected, and described wire is led from described
Line position limiting structure passes through;
Upper cover, described upper cover is covered on described base hermetically, described on be covered with for Pei Yangye sample molten
The interface that liquid enters and discharges.
The lower surface and the sidewall that run through described upper cover are provided with the conductive hole for being drawn by described conductor wire.
Also including sealing ring, the top edge of the corresponding described support seat of the lower surface of described upper cover arranges described sealing
Circle.
Also include that temperature control equipment, described temperature control equipment are located at bottom described cavity, described support seat
The material good for heat conductivility is made, across described support seat to described quartz between described temperature control equipment
The temperature of oscillator is controlled.
Described support includes that circumferential body structure, described circumferential body structure are convexly equipped with flange to direction, the center of circle,
Described quartz vibrator is carried on described flange.
The outer rim of described flange connects has network structure, described quartz vibrator to be carried on described flange and described net
In shape structure.
Described wire restraint structure is for being located at described support two ends, and runs through what described support upper and lower surface was arranged
Allow the through hole that described wire passes.
Described wire restraint structure is to be located at the breach at described support two ends, and described wire restraint is in described breach
With described circular trough sidewall besieged city space in.
Also include that crystal oscillator device fixed structure, described crystal oscillator device fixed structure include being located at described rounded trough bottom
The locating dowel in portion and the hole, location coordinated with described locating dowel.
Compared with prior art, owing to having the culture dish for microorganism detection of reference function in the present invention
In, described crystal oscillator device includes support, quartz vibrator, the rounded structure of described support, described quartz vibrator
Being embedded on described support, the two ends of described support further respectively have wire restraint structure, described quartz vibrator
It is laid with foil-like exciting electrode at tow sides, and described exciting electrode is set as circle with external circuit junction
Disk shape.Therefore, the described quartz vibrator in the present invention can preferably be fixed, it is possible to prevents described stone
English oscillator in the course of the work, occurs being connected the situation of instability with described electric wire, is furthermore possible to improve
Described quartz vibrator keeps balance when vibration, is favorably improved the standard that described quartz vibrator frequency of vibration is measured
Really property.
By description below and combine accompanying drawing, the present invention will become more fully apparent, and these accompanying drawings are used for explaining
Embodiments of the invention.
Accompanying drawing explanation
Fig. 1 is that the present invention has showing of an embodiment of the culture dish for microorganism detection of reference function
It is intended to.
Fig. 2 is the explosive view of the culture dish for microorganism detection with reference function as shown in Figure 1.
Fig. 3 is the quartz vibrator of the culture dish for microorganism detection with reference function as shown in Figure 1
Structural representation.
Fig. 4 is the one of the support of the culture dish for microorganism detection with reference function as shown in Figure 1
The schematic diagram of individual embodiment.
Fig. 5 is the another of the support of the culture dish for microorganism detection with reference function as shown in Figure 1
The schematic diagram of one embodiment.
Fig. 6 is the crystal oscillator device of the culture dish for microorganism detection with reference function as shown in Figure 1
The schematic diagram coordinated with support seat.
Detailed description of the invention
With reference now to accompanying drawing, describing embodiments of the invention, element numbers similar in accompanying drawing represents similar unit
Part.As it has been described above, as shown in Figure 1, 2, 3, what the embodiment of the present invention provided have reference function for
The culture dish 100 of microorganism detection, including:
Base 1, described base 1 is formed a cavity 10 by upper surface concave downward;
Supporting seat 2, described support seat 2 is contained in described cavity 10, and described support seat 2 upper surface sets
There is a circular trough 20;
Crystal oscillator device 3, described crystal oscillator device is fixed in described circular trough 20, shakes including support 30, quartz
Son 31, the described rounded structure of support 30, described quartz vibrator 31 is embedded on described support 30, described
The two ends of support 30 further respectively have wire restraint structure 32, and described quartz vibrator 31 is set as the first vibrating area
Territory 310 and the second vibration area 311, described first vibration area 310 and the second vibration area 311 positive and negative
Two sides is laid with foil-like exciting electrode 33, and described exciting electrode 33 is set as disk with external circuit junction
Shape 34, this disk-like shape 34 is able to improve contact area, is positioned at described first vibration area
Molding antibody layer 312 on the described exciting electrode 33 of 310, is positioned at the institute on described second vibration area 311
State molding barrier layer 313 on exciting electrode 33;The side arranging described barrier layer 313 does not has the suction of microorganism
Attached, therefore described in test process, the change of the frequency of vibration of the second vibration area 311 is described first to shake
The reference of the frequency of vibration change in dynamic region 310, the change of the frequency of vibration of described second vibration area 311
Should tend towards stability, and its change should also be and produces change due to temperature factor.Therefore test
During, exhausted by the difference of described first vibration area 310 and the frequency of vibration of the second vibration area 311
To value, it is possible to offset the frequency change produced with environmental change, it is possible to take out only being adsorbed as with microorganism
The change of the frequency of oscillation of cause.
Electric wire 4, described electric wire 4 includes wire 40 and is connected to the plate-like of described wire 40 end
Clamp structure 41, described plate-like clamp structure 41 is clamped on described disc-shape 34 and is electrically connected,
And described wire 40 passes through from described wire restraint structure 32;
Upper cover 5, described upper cover 5 is covered on described base 1 hermetically, and described upper cover 5 is provided with for culture fluid
Sample solution enter and discharge interface 50.
The lower surface and the sidewall that run through described upper cover 5 are provided with the conductive hole 51 for being drawn by described wire 40.
As in figure 2 it is shown, also include sealing ring 6, the corresponding described support seat 2 of the lower surface of described upper cover 5 upper
Edge arranges described sealing ring 6.When making described upper cover 5 be covered on described base 1 by described sealing ring 6,
It is collectively forming a sealing space.
Also include that temperature control equipment 7, described temperature control equipment 7 are located at bottom described cavity 10, described
Supporting seat 2 is that the material that heat conductivility is good is made, across described support seat 2 between described temperature control equipment 7
The temperature of described quartz vibrator 31 is controlled.
As shown in Figure 4, described support 30 includes circumferential body structure 300, described circumferential body structure 300
Be convexly equipped with flange 301 to direction, the center of circle, described quartz vibrator 31 is carried on described flange 301.
As shown in Figure 4, the outer rim of described flange 301 connects network structure 302, described quartz vibrator 31
It is carried in described flange 301 and described network structure 302.
As shown in Figure 4, described wire restraint structure 32 is for being located at described support 30 two ends, and runs through described
What support 30 upper and lower surface was arranged the allow through hole that described wire 40 passes.
As it is shown in figure 5, described wire restraint structure 32 is to be located at the breach at described support two ends, described wire
In 40 spaces being limited in described breach and described circular trough 20 sidewall besieged city.
As shown in Figure 6, also include that crystal oscillator device fixed structure 8, described crystal oscillator device fixed structure 8 include setting
Locating dowel 80 bottom described circular trough 20 and the hole, location 81 coordinated with described locating dowel 80.
Compared with prior art, in conjunction with Fig. 1~6, due to the present invention have reference function for microorganism
The culture dish 100 of detection, described crystal oscillator device includes support 30, quartz vibrator 31, and described support 30 is in circle
Shape structure, described quartz vibrator 31 is embedded on described support, and the two ends of described support 30 further respectively have
Wire restraint structure 32, described quartz vibrator 31 is laid with foil-like exciting electrode 33 at tow sides, and
Described exciting electrode 33 is set as disc-shape 34, therefore, the described stone in the present invention with external circuit junction
English oscillator can preferably be fixed, it is possible to prevents described quartz vibrator in the course of the work, occurs with described
Electric wire connects unstable situation, is furthermore possible to improve described quartz vibrator and keeps balance when vibration,
It is favorably improved the accuracy that described quartz vibrator frequency of vibration is measured.
Above disclosed only the preferred embodiments of the present invention, can not limit the present invention with this certainly
Interest field, the equivalent variations therefore made according to scope of the present invention patent, still belong to the present invention and contained
Scope.
Claims (9)
1. the culture dish for microorganism detection with reference function, it is characterised in that including:
Base, base is formed a cavity by upper surface concave downward;
Supporting seat, described support seat is contained in described cavity, and described support seat upper surface is provided with a circle
Groove;
Crystal oscillator device, described crystal oscillator device is fixed in described circular trough, including support, quartz vibrator, institute
Stating the rounded structure of support, described quartz vibrator is embedded on described support, and the two ends of described support are also distinguished
Being provided with wire restraint structure, described quartz vibrator is set as the first vibration area and the second vibration area, and described
The tow sides of one vibration area and the second vibration area are laid with foil-like exciting electrode, and described excitation electricity
Pole and external circuit junction are set as disc-shape, are positioned on the described exciting electrode of described first vibration area
Molding antibody layer, is positioned at molding barrier layer on the described exciting electrode on described second vibration area;
Electric wire, described electric wire includes wire and is connected to the plate-like clamp structure of described wire end,
Described plate-like clamp structure is clamped on described disc-shape and is electrically connected, and described wire is led from described
Line position limiting structure passes through;
Upper cover, described upper cover is covered on described base hermetically, described on be covered with for Pei Yangye sample molten
The interface that liquid enters and discharges.
There is the culture dish for microorganism detection of reference function the most as claimed in claim 1, its feature
Being, the lower surface and the sidewall that run through described upper cover are provided with the conductive hole for being drawn by described conductor wire.
There is the culture dish for microorganism detection of reference function the most as claimed in claim 1, its feature
Being, also include sealing ring, the top edge of the corresponding described support seat of the lower surface of described upper cover arranges described close
Seal.
There is the culture dish for microorganism detection of reference function the most as claimed in claim 1, its feature
It is, also includes that temperature control equipment, described temperature control equipment are located at bottom described cavity, described support
Seat is made, across described support seat to described stone between described temperature control equipment for the material that heat conductivility is good
The temperature of English oscillator is controlled.
There is the culture dish for microorganism detection of reference function the most as claimed in claim 1, its feature
Being, described support includes that circumferential body structure, described circumferential body structure are convexly equipped with flange to direction, the center of circle,
Described quartz vibrator is carried on described flange.
There is the culture dish for microorganism detection of reference function the most as claimed in claim 5, its feature
Being, the outer rim of described flange connects has network structure, described quartz vibrator to be carried on described flange and described
In network structure.
There is the culture dish for microorganism detection of reference function the most as claimed in claim 1, its feature
Being, described wire restraint structure is for being located at described support two ends, and runs through the setting of described support upper and lower surface
Allow the through hole that described wire passes.
There is the culture dish for microorganism detection of reference function the most as claimed in claim 1, its feature
Being, described wire restraint structure is to be located at the breach at described support two ends, and described wire restraint lacks in described
In the space that mouth and described circular trough sidewall are encircled a city.
There is the culture dish for microorganism detection of reference function the most as claimed in claim 1, its feature
It is, also includes that crystal oscillator device fixed structure, described crystal oscillator device fixed structure include being located at described circular trough
The locating dowel of bottom and the hole, location that coordinates with described locating dowel.
Priority Applications (1)
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CN201610570850.4A CN106010960B (en) | 2016-07-19 | 2016-07-19 | With the culture dish for microorganism detection referring to function |
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CN201610570850.4A CN106010960B (en) | 2016-07-19 | 2016-07-19 | With the culture dish for microorganism detection referring to function |
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CN106010960A true CN106010960A (en) | 2016-10-12 |
CN106010960B CN106010960B (en) | 2019-08-09 |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106918582A (en) * | 2017-03-31 | 2017-07-04 | 华测检测认证集团股份有限公司 | Microbial detection device |
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JP2006029873A (en) * | 2004-07-13 | 2006-02-02 | Nippon Dempa Kogyo Co Ltd | Quartz sensor and sensing device |
CN101080625A (en) * | 2004-12-15 | 2007-11-28 | 日本电波工业株式会社 | Component measuring instrument |
CN101084427A (en) * | 2004-12-15 | 2007-12-05 | 日本电波工业株式会社 | Crystal sensor and sensing device |
CN102192860A (en) * | 2010-03-10 | 2011-09-21 | 日本电波工业株式会社 | Method of detecting microorganisms and microorganism detecting apparatus |
CN205990402U (en) * | 2016-07-19 | 2017-03-01 | 华测检测认证集团股份有限公司 | There is the culture dish for microorganism detection of reference function |
-
2016
- 2016-07-19 CN CN201610570850.4A patent/CN106010960B/en active Active
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
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JPH09145583A (en) * | 1995-11-14 | 1997-06-06 | Dev Center For Biotechnol | Piezoelectric sensor chip of cartridge-form |
JP2006029873A (en) * | 2004-07-13 | 2006-02-02 | Nippon Dempa Kogyo Co Ltd | Quartz sensor and sensing device |
CN101080625A (en) * | 2004-12-15 | 2007-11-28 | 日本电波工业株式会社 | Component measuring instrument |
CN101084427A (en) * | 2004-12-15 | 2007-12-05 | 日本电波工业株式会社 | Crystal sensor and sensing device |
CN102192860A (en) * | 2010-03-10 | 2011-09-21 | 日本电波工业株式会社 | Method of detecting microorganisms and microorganism detecting apparatus |
CN205990402U (en) * | 2016-07-19 | 2017-03-01 | 华测检测认证集团股份有限公司 | There is the culture dish for microorganism detection of reference function |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN106918582A (en) * | 2017-03-31 | 2017-07-04 | 华测检测认证集团股份有限公司 | Microbial detection device |
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Effective date of registration: 20210309 Address after: 518101 Hua Cheng Building, No. 4, Liuxian Third Road, Xin'an Street, Baoan District, Shenzhen City, Guangdong Province Patentee after: SHENZHEN CTI LABORATORY Co.,Ltd. Address before: 518101 C building, Hongwei Industrial Park, 70 District, Guangdong, Shenzhen, Baoan District Patentee before: CENTRE TESTING INTERNATIONAL GROUP Co.,Ltd. |