CN106010941A - Culture apparatus for microbiological detection - Google Patents
Culture apparatus for microbiological detection Download PDFInfo
- Publication number
- CN106010941A CN106010941A CN201610571854.4A CN201610571854A CN106010941A CN 106010941 A CN106010941 A CN 106010941A CN 201610571854 A CN201610571854 A CN 201610571854A CN 106010941 A CN106010941 A CN 106010941A
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- China
- Prior art keywords
- culture apparatus
- wire
- microorganism detection
- support
- crystal oscillator
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Sustainable Development (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Thermal Sciences (AREA)
- Physics & Mathematics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a culture apparatus for microbiological detection. The culture apparatus comprises a base, a supporter, a crystal oscillator, conductive wires and a top cover. The upper surface of the base is sunken to form a cavity; the supporter is accommodated in the cavity; and the upper surface of the supporter is provided with a circular groove. The crystal oscillator consists of a holder and a quartz oscillator; the holder is of a circular structure, the quartz oscillator is embedded in the holder, both ends of the holder are provided with conductor limit structures, foil-type exciting electrodes are uniformly arranged on the front and back sides of the quartz oscillator respectively, and the joint between each exciting electrode and an external circuit is designed into a disc-shaped structure. The conductive wires include conductors and disc-like clamp structures connected to tail ends of the conductors; the disc-like clamp structures are clamped to the disc-shaped structures to form electrical connection; and the conductors penetrate the conductor limit structures. The top cover seals the base and is provided with connectors for feeding and discharging of culture solution/sample solution. The culture apparatus for microbiological detection has the advantages of shortening culture cycle and improving detection accuracy.
Description
Technical field
The present invention relates to technical field of microbial detection, particularly relate to one and can shorten incubation time, improve
The microbial cultivation device of detection accuracy.
Background technology
Along with expanding economy, improving constantly of people's living standard, for the consciousness of food safety also not
Improve disconnectedly.In the past, as carrying out the method that whether contains the judgement of putrefaction bacteria in Foods or drinks, bag
Include use immunity measurement method, ELISA method, gas-chromatography mass analysis, liquid chromatographic mass analytic process etc.
Machine survey method, but measure pre-treatment numerous and diverse, it is determined that precision can not say fully.Thus, generally to spend
Expense cultivates putrefaction bacteria for a long time.
The technology including labelling microorganism, the light sent with prominent microorganism and growth holder is used to send
Contrast between light.Owing to using the optical system of the sensitivity of the feature (such as wavelength or intensity) to the light sent
System, so using specific fluorescent labeling, fluorogen or use can expose the biology sent by such as ATP
The enzyme of fluorescence, can carry out getting up early detection to microorganism.
Additionally, being cultivated by agar culture medium is one of most common and the most ancient method, wherein culture medium
It is more or less selectable for one or more microorganism type.In the period that generally can extend to several days
In, this culture medium is cultivated at a proper temperature, for the microorganism that growth is to be found.This cultivation
The shortcoming of method remains the bacterium colony formed on agar can carry out the visible detection of bore hole, needs relatively long
Cultivation cycle.Will also realize that from current technological level, DNA sequence or the breeding of RNA sequence can be passed through
Perform chain reaction (also referred to as PCR breeding), so that it is determined that sample exists specified microorganisms.These methods
Shortcoming be: in order to enable on agar culture medium formed bacterium colony carry out the visible detection of bore hole, need phase
To long cultivation cycle.And will also realize that from current technological level, NDA sequence or RNA can be passed through
The breeding of sequence performs chain polymerization reaction (also referred to as PCR breeding), so that it is determined that the specified microorganisms in sample.
The shortcoming of these methods is to need several DNA, the most several microorganism, it is generally required at least tens kinds
Microorganism, the method that this method is not based on growth is sensitive.
It is relatively common that agar culture medium cultivates the method for microorganism to be detected, but its weak point
Be cultivate overlong time, how can shorten incubation time, improve efficiency become need break through bottleneck.
Summary of the invention
It is an object of the invention to provide one and can shorten incubation time, improve the microorganism training of detection accuracy
Support device.
To achieve these goals, the technical scheme that the present invention provides is: provide one for microorganism detection
Culture apparatus, including:
Base, described base is formed a cavity by upper surface concave downward；
Supporting seat, described support seat is contained in described cavity, and described support seat upper surface is provided with a circle
Groove；
Crystal oscillator device, described crystal oscillator device is fixed in described circular trough, including support, quartz vibrator, institute
Stating the rounded structure of support, described quartz vibrator is embedded on described support, and the two ends of described support are also distinguished
Being provided with wire restraint structure, described quartz vibrator is laid with foil-like exciting electrode at tow sides, and described
Exciting electrode and external circuit junction are set as disc-shape；
Electric wire, described electric wire includes wire and is connected to the plate-like clamp structure of described wire end,
Described plate-like clamp structure is clamped on described disc-shape and is electrically connected, and described wire is led from described
Line position limiting structure passes through；
Upper cover, described upper cover is covered on described base hermetically, described on be covered with for Pei Yangye sample molten
The interface that liquid enters and discharges.
The lower surface and the sidewall that run through described upper cover are provided with the conductive hole for being drawn by described conductor wire.
Also including sealing ring, the top edge of the corresponding described support seat of the lower surface of described upper cover arranges described sealing
Circle.
Also include that temperature control equipment, described temperature control equipment are located at bottom described cavity, described support seat
The material good for heat conductivility is made, across described support seat to described quartz between described temperature control equipment
The temperature of oscillator is controlled.
Described support includes that circumferential body structure, described circumferential body structure are convexly equipped with flange to direction, the center of circle,
Described quartz vibrator is carried on described flange.
Described wire restraint structure is for being located at described support two ends, and runs through what described support upper and lower surface was arranged
Allow the through hole that described wire passes.
Described wire restraint structure is to be located at the breach at described support two ends, and described wire restraint is in described breach
With described circular trough sidewall besieged city space in.
Also include that crystal oscillator device fixed structure, described crystal oscillator device fixed structure include being located at described rounded trough bottom
The locating dowel in portion and the hole, location coordinated with described locating dowel.
Compared with prior art, owing to being used in the present invention in the culture apparatus of microorganism detection, described crystal oscillator
Device includes support, quartz vibrator, the rounded structure of described support, and described quartz vibrator is embedded in described
On frame, the two ends of described support further respectively have wire restraint structure, and described quartz vibrator is equal at tow sides
It is laid with foil-like exciting electrode, and described exciting electrode is set as disc-shape with external circuit junction.Therefore,
Described quartz vibrator in the present invention can preferably be fixed, it is possible to prevents described quartz vibrator worked
, there is being connected with described electric wire the situation of instability, is furthermore possible to improve described quartz vibrator and exists in Cheng Zhong
Keep balance during vibration, be favorably improved the accuracy that described quartz vibrator frequency of vibration is measured.
By description below and combine accompanying drawing, the present invention will become more fully apparent, and these accompanying drawings are used for explaining
Embodiments of the invention.
Accompanying drawing explanation
Fig. 1 is the present invention schematic diagram for an embodiment of the culture apparatus of microorganism detection.
Fig. 2 is the explosive view of the culture apparatus for microorganism detection as shown in Figure 1.
Fig. 3 is showing of an embodiment of the support of the culture apparatus for microorganism detection as shown in Figure 1
It is intended to.
Fig. 4 is another embodiment of the support of the culture apparatus for microorganism detection as shown in Figure 1
Schematic diagram.
Fig. 5 is that the crystal oscillator device of the culture apparatus for microorganism detection as shown in Figure 1 coordinates with supporting seat
Schematic diagram.
Detailed description of the invention
With reference now to accompanying drawing, describing embodiments of the invention, element numbers similar in accompanying drawing represents similar unit
Part.As it has been described above, as illustrated in fig. 1 and 2, the cultivation for microorganism detection that the embodiment of the present invention provides
Device 100, including:
Base 1, described base 1 is formed a cavity 10 by upper surface concave downward；
Supporting seat 2, described support seat 2 is contained in described cavity 10, and described support seat 2 upper surface sets
There is a circular trough 20；
Crystal oscillator device 3, described crystal oscillator device is fixed in described circular trough 20, shakes including support 30, quartz
Son 31, the described rounded structure of support 30, described quartz vibrator 31 is embedded on described support 30, described
The two ends of support 30 further respectively have wire restraint structure 32, and described quartz vibrator 31 is uniform at tow sides
It is provided with foil-like exciting electrode 33, and described exciting electrode 33 is set as disc-shape 34 with external circuit junction,
This disk-like shape 34 is able to improve contact area；
Electric wire 4, described electric wire 4 includes wire 40 and is connected to the plate-like of described wire 40 end
Clamp structure 41, described plate-like clamp structure 41 is clamped on described disc-shape 34 and is electrically connected,
And described wire 40 passes through from described wire restraint structure 32；
Upper cover 5, described upper cover 5 is covered on described base 1 hermetically, and described upper cover 5 is provided with for culture fluid
Sample solution enter and discharge interface 50.
The lower surface and the sidewall that run through described upper cover 5 are provided with the conductive hole 51 for being drawn by described wire 40.
As in figure 2 it is shown, also include sealing ring 6, the corresponding described support seat 2 of the lower surface of described upper cover 5 upper
Edge arranges described sealing ring 6.When making described upper cover 5 be covered on described base 1 by described sealing ring 6,
It is collectively forming a sealing space.
Also include that temperature control equipment 7, described temperature control equipment 7 are located at bottom described cavity 10, described
Supporting seat 2 is that the material that heat conductivility is good is made, across described support seat 2 between described temperature control equipment 7
The temperature of described quartz vibrator 31 is controlled.
As it is shown on figure 3, described support 30 includes circumferential body structure 300, described circumferential body structure 300
Be convexly equipped with flange 301 to direction, the center of circle, described quartz vibrator 31 is carried on described flange 301.
As it is shown on figure 3, described wire restraint structure 32 is for being located at described support 30 two ends, and run through described
What support 30 upper and lower surface was arranged the allow through hole that described wire 40 passes.
As shown in Figure 4, described wire restraint structure 32 is to be located at the breach at described support two ends, described wire
In 40 spaces being limited in described breach and described circular trough 20 sidewall besieged city.
As it is shown in figure 5, also include crystal oscillator device fixed structure 8, described crystal oscillator device fixed structure 8 includes setting
Locating dowel 80 bottom described circular trough 20 and the hole, location 81 coordinated with described locating dowel 80.
Compared with prior art, in conjunction with Fig. 1～5, owing to being used for the culture apparatus of microorganism detection in the present invention
100, described crystal oscillator device includes support 30, quartz vibrator 31, and the described rounded structure of support 30 is described
Quartz vibrator 31 is embedded on described support, and the two ends of described support 30 further respectively have wire restraint structure
32, described quartz vibrator 31 is laid with foil-like exciting electrode 33, and described exciting electrode at tow sides
33 are set as disc-shape 34 with external circuit junction, and therefore, the described quartz vibrator in the present invention can be more
Fixed well, it is possible to prevent described quartz vibrator in the course of the work, occur being connected with described electric wire
Unstable situation, is furthermore possible to improve described quartz vibrator and keeps balance when vibration, be favorably improved institute
State the accuracy that quartz vibrator frequency of vibration is measured.
Above disclosed only the preferred embodiments of the present invention, can not limit the present invention with this certainly
Interest field, the equivalent variations therefore made according to scope of the present invention patent, still belong to the present invention and contained
Scope.
Claims (8)
1. the culture apparatus for microorganism detection, it is characterised in that including:
Base, base is formed a cavity by upper surface concave downward；
Supporting seat, described support seat is contained in described cavity, and described support seat upper surface is provided with a circle
Groove；
Crystal oscillator device, described crystal oscillator device is fixed in described circular trough, including support, quartz vibrator, institute
Stating the rounded structure of support, described quartz vibrator is embedded on described support, and the two ends of described support are also distinguished
Being provided with wire restraint structure, described quartz vibrator is laid with foil-like exciting electrode at tow sides, and described
Exciting electrode and external circuit junction are set as disc-shape；
Electric wire, described electric wire includes wire and is connected to the plate-like clamp structure of described wire end,
Described plate-like clamp structure is clamped on described disc-shape and is electrically connected, and described wire is led from described
Line position limiting structure passes through；
Upper cover, described upper cover is covered on described base hermetically, described on be covered with for Pei Yangye sample molten
The interface that liquid enters and discharges.
2. the culture apparatus for microorganism detection as claimed in claim 1, it is characterised in that run through institute
Lower surface and the sidewall of stating upper cover are provided with the conductive hole for being drawn by described conductor wire.
3. the culture apparatus for microorganism detection as claimed in claim 1, it is characterised in that also include
Sealing ring, the top edge of the corresponding described support seat of the lower surface of described upper cover arranges described sealing ring.
4. the culture apparatus for microorganism detection as claimed in claim 1, it is characterised in that also include
Temperature control equipment, described temperature control equipment is located at bottom described cavity, and described support seat is heat conductivility
Good material is made, across the described support seat temperature to described quartz vibrator between described temperature control equipment
It is controlled.
5. the culture apparatus for microorganism detection as claimed in claim 1, it is characterised in that described
Frame includes circumferential body structure, and described circumferential body structure is convexly equipped with flange to direction, the center of circle, and described quartz shakes
Son is carried on described flange.
6. the culture apparatus for microorganism detection as claimed in claim 1, it is characterised in that described in lead
Line position limiting structure is for being located at described support two ends, and runs through the described wire that allows of described support upper and lower surface setting
The through hole passed.
7. the culture apparatus for microorganism detection as claimed in claim 1, it is characterised in that described in lead
Line position limiting structure is the breach being located at described support two ends, and described wire restraint is in described breach and described circle
In the space in groove sidewall besieged city.
8. the culture apparatus for microorganism detection as claimed in claim 1, it is characterised in that also include
Crystal oscillator device fixed structure, described crystal oscillator device fixed structure include being located at the locating dowel bottom described circular trough,
And the hole, location coordinated with described locating dowel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610571854.4A CN106010941B (en) | 2016-07-19 | 2016-07-19 | Culture apparatus for microorganism detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610571854.4A CN106010941B (en) | 2016-07-19 | 2016-07-19 | Culture apparatus for microorganism detection |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106010941A true CN106010941A (en) | 2016-10-12 |
| CN106010941B CN106010941B (en) | 2019-08-09 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610571854.4A Expired - Fee Related CN106010941B (en) | 2016-07-19 | 2016-07-19 | Culture apparatus for microorganism detection |
Country Status (1)
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| CN (1) | CN106010941B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106893671A (en) * | 2017-03-31 | 2017-06-27 | 华测检测认证集团股份有限公司 | Micro-organism test apparatus |
| CN106918582A (en) * | 2017-03-31 | 2017-07-04 | 华测检测认证集团股份有限公司 | Microbial detection device |
| CN107881087A (en) * | 2017-12-26 | 2018-04-06 | 华测检测认证集团股份有限公司 | Detection means for food microorganisms |
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| JPH09145583A (en) * | 1995-11-14 | 1997-06-06 | Dev Center For Biotechnol | Piezoelectric sensor chip of cartridge-form |
| JP2006029873A (en) * | 2004-07-13 | 2006-02-02 | Nippon Dempa Kogyo Co Ltd | Quartz sensor and sensing device |
| CN101080625A (en) * | 2004-12-15 | 2007-11-28 | 日本电波工业株式会社 | Component measuring instrument |
| CN101084427A (en) * | 2004-12-15 | 2007-12-05 | 日本电波工业株式会社 | Crystal sensor and sensing device |
| CN102192860A (en) * | 2010-03-10 | 2011-09-21 | 日本电波工业株式会社 | Method of detecting microorganisms and microorganism detecting apparatus |
| CN205990392U (en) * | 2016-07-19 | 2017-03-01 | 华测检测认证集团股份有限公司 | Culture apparatuses for microorganism detection |
-
2016
- 2016-07-19 CN CN201610571854.4A patent/CN106010941B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09145583A (en) * | 1995-11-14 | 1997-06-06 | Dev Center For Biotechnol | Piezoelectric sensor chip of cartridge-form |
| JP2006029873A (en) * | 2004-07-13 | 2006-02-02 | Nippon Dempa Kogyo Co Ltd | Quartz sensor and sensing device |
| CN101080625A (en) * | 2004-12-15 | 2007-11-28 | 日本电波工业株式会社 | Component measuring instrument |
| CN101084427A (en) * | 2004-12-15 | 2007-12-05 | 日本电波工业株式会社 | Crystal sensor and sensing device |
| CN102192860A (en) * | 2010-03-10 | 2011-09-21 | 日本电波工业株式会社 | Method of detecting microorganisms and microorganism detecting apparatus |
| CN205990392U (en) * | 2016-07-19 | 2017-03-01 | 华测检测认证集团股份有限公司 | Culture apparatuses for microorganism detection |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106893671A (en) * | 2017-03-31 | 2017-06-27 | 华测检测认证集团股份有限公司 | Micro-organism test apparatus |
| CN106918582A (en) * | 2017-03-31 | 2017-07-04 | 华测检测认证集团股份有限公司 | Microbial detection device |
| CN107881087A (en) * | 2017-12-26 | 2018-04-06 | 华测检测认证集团股份有限公司 | Detection means for food microorganisms |
| CN107881087B (en) * | 2017-12-26 | 2023-08-08 | 华测检测认证集团股份有限公司 | Detection device for food microorganisms |
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| Publication number | Publication date |
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| CN106010941B (en) | 2019-08-09 |
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Granted publication date: 20190809 Termination date: 20210719 |