CN106008741A - Preparation method of yeast beta-glucan - Google Patents
Preparation method of yeast beta-glucan Download PDFInfo
- Publication number
- CN106008741A CN106008741A CN201610414670.7A CN201610414670A CN106008741A CN 106008741 A CN106008741 A CN 106008741A CN 201610414670 A CN201610414670 A CN 201610414670A CN 106008741 A CN106008741 A CN 106008741A
- Authority
- CN
- China
- Prior art keywords
- acid
- preparation
- yeast beta
- dextran
- described step
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0024—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Glucans; (beta-1,3)-D-Glucans, e.g. paramylon, coriolan, sclerotan, pachyman, callose, scleroglucan, schizophyllan, laminaran, lentinan or curdlan; (beta-1,6)-D-Glucans, e.g. pustulan; (beta-1,4)-D-Glucans; (beta-1,3)(beta-1,4)-D-Glucans, e.g. lichenan; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K5/00—Use of organic ingredients
- C08K5/04—Oxygen-containing compounds
- C08K5/13—Phenols; Phenolates
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K5/00—Use of organic ingredients
- C08K5/04—Oxygen-containing compounds
- C08K5/13—Phenols; Phenolates
- C08K5/132—Phenols containing keto groups, e.g. benzophenones
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K2201/00—Specific properties of additives
- C08K2201/014—Additives containing two or more different additives of the same subgroup in C08K
Abstract
The invention discloses a preparation method of yeast beta-glucan. The preparation method comprises the following steps that 1, 8-12 parts by weight of saccharomyces cerevisiae is added into 35-85 parts by weight of enzyme solution, the pH is adjusted to be 5 to 10, autolysis is induced for 12 h to 28 h under water bath at 48 DEG C to 62 DEG C, enzyme denaturalixation is conducted, and enzymatic hydrolysate is obtained; 2, the enzymatic hydrolysate is centrifuged and washed to obtain sediments, the sediments are added into 12-28 parts by weight of acid liquor, acid hydrolysis is conducted for 1 h to 5 h at 70 DEG C to 120 DEG C, centrifugation and washing are conducted, and acid hydrolysate is obtained; 3, the acid hydrolysate is washed with ethyl alcohol one to three times, filtration is conducted, and filter residues are obtained; 4, the filter residues are mixed with preservative according to the mass ratio of 100:(0.01-0.1), mixed powder is obtained and dried, and the yeast beta-glucan is obtained. According to the preparation method of the yeast beta-glucan, the purity of the obtained product is high, the process is simple, the functions of enhancing immunity and resisting radiation and senescence are achieved, and a good application prospect is achieved.
Description
Technical field
The present invention relates to nutraceutical field, be specifically related to the preparation method of a kind of yeast beta-dextran.
Background technology
Yeast dextran be present in yeast cell wall a kind of have enhancing immunity activity polysaccharide β-
Glucosan.Beta glucan is widely present in various fungus and plant, in Lentinus Edodes, Ganoderma, Herba bromi japonici, is
They play the primary efficacy material of health-care effect.And the immune-enhancing activity of yeast dextran is higher, and
Have and improve blood fat, radioprotective, enhancing immunity, reduce blood fat, improve the effect of function of intestinal canal.Yeast
Glucosan is widely used in various food (meat products, milk product, cookies, beverage, fruit juice), various
Cosmetic raw material (shampoo, bath gel, facial film, hand cream etc.), health food and pharmaceutical raw material.
The preparation method of saccharomyces cerevisiae beta glucan is broadly divided into acid system, alkaline process, acid-base method, supercritical ultrasonics technology
With combined techniques etc..
The invention provides the preparation method of a kind of yeast beta-dextran, the comprehensive utilization for yeast has weight
Want meaning.
Summary of the invention
For above-mentioned deficiency present in prior art, the technical problem to be solved is to provide one
The preparation method of primary yeast beta glucan.
The present invention seeks to be achieved through the following technical solutions:
The preparation method of a kind of yeast beta-dextran, comprises the following steps:
(1) being added by the saccharomyces cerevisiae of 8-12 weight portion in the enzyme liquid of 35-85 weight portion, regulation pH is extremely
5-10, induces self-dissolving 12-28 hour under 48-62 DEG C of water-bath, and enzyme denaturing is lived, obtained enzymolysis solution；
(2) enzymolysis solution is centrifuged, washing, is precipitated thing, precipitate is joined 12-28 weight portion
Acid solution in, acidolysis 1-5 hour at 70-120 DEG C, centrifugal, washing, obtain acidolysis thing；
(3) by acidolysis thing washing with alcohol 1-3 time, filter, obtain filtering residue,
(4) by filtering residue with antistaling agent it is 100:(0.01-0.1 in mass ratio) mix, obtain mixed powder,
Dry, to obtain final product.
In described step (1), regulation pH value can use NaOH solution, HCl solution etc..
Preferably, the enzyme liquid in described step (1) is made up of the raw material of following mass percent: Fructus Chaenomelis egg
White enzyme 1-15%, water 87-97%.
Preferably, the acid solution in described step (2) is made up of the raw material of following mass percent: tartaric acid
0.2-2%, succinic acid 0.2-2%, citric acid 0.2-2%, water 94-99%.
Preferably, the flame filter press that is filtered in described step (3) filters, and uses 150-200 purpose
Filter cloth.
Preferably, the antistaling agent of described step (4) is mixed by carvacrol, paeonol, eugenol,
Described carvacrol, paeonol, the mass ratio of eugenol are (1-3): (1-3): (1-3).
The yeast beta-dextran preparation method that the present invention provides, the product purity obtained is high, and technique is simple,
And there is the function of enhancing immunity, radioprotective, defying age, have a good application prospect.
Detailed description of the invention
Each raw material introduction in embodiment:
Tartaric acid, No. CAS: 526-83-0.
Succinic acid, No. CAS: 110-15-6.
Citric acid, No. CAS: 77-92-9.
Carvacrol, No. CAS: 499-75-2.
Paeonol, No. CAS: 552-41-0.
Eugenol, No. CAS: 97-53-0
Papain, No. CAS: 9001-73-4, Zhengzhou Kang Yuan chemical products company limited provides, enzyme
Live 200U/mg.
Saccharomyces cerevisiae, uses the saccharomyces cerevisiae that Shandong Long Xing biological engineering company limited provides.
Embodiment 1
The concrete preparation method of yeast beta-dextran, comprises the following steps:
(1) saccharomyces cerevisiae of 100g is added in the enzyme liquid of 500g and be uniformly mixed, and use 2mol/L
Sodium hydroxide solution regulation pH to 7.0, under 55 DEG C of water-baths, induce self-dissolving 24 hours, then at 95 DEG C
Under water-bath, enzyme denaturing is lived 10 minutes, obtains enzymolysis solution；
(2) 5000 revs/min of centrifuge of enzymolysis solution is centrifuged 10 minutes, is precipitated thing a；Will precipitation
After thing a joins 200g distilled water 300 revs/min stirring 5 minutes, it is centrifuged with 5000 revs/min of centrifuge
10 minutes, it is precipitated thing b；Precipitate b is joined 200g distilled water 300 revs/min and stirs 5 points
Zhong Hou, is centrifuged 10 minutes with 5000 revs/min of centrifuge, is precipitated thing c, is joined by precipitate c
In the acid solution of 200g, acidolysis 2 hours at 80 DEG C, it is centrifuged 10 minutes with 5000 revs/min of centrifuge,
Obtain acidolysis thing a；Acidolysis thing a joins 200g distilled water 300 revs/min stir after 5 minutes, with from
Centrifugal 10 minutes of scheming 5000 revs/min, obtains acidolysis thing b；Acidolysis thing b is joined 200g distillation
After 300 revs/min of water stirs 5 minutes, it is centrifuged 10 minutes with 5000 revs/min of centrifuge, obtains acidolysis thing c；
(3), after acidolysis thing c being joined in 100g ethanol 300 revs/min of stirrings 5 minutes, sheet frame mistake is used
Filter uses the filter-cloth filtering of 180 mesh, obtains filtering residue；
(4) filtering residue is mixed homogeneously with antistaling agent 100:0.03 in mass ratio, lyophilizing at-30 DEG C.?
Yeast beta-dextran to embodiment 1.
Enzyme liquid in described step (1) is made up of the raw material of following mass percent: papain 5%,
Distilled water 94%；Join papain in distillation to be uniformly mixed and i.e. obtain enzyme liquid.
Acid solution in described step (2) is made up of the raw material of following mass percent: tartaric acid 0.8%,
Succinic acid 0.8%, citric acid 0.8%, distilled water 97.6%；Tartaric acid, succinic acid, citric acid are added
It is uniformly mixed in distilled water and i.e. obtains acid solution.
The antistaling agent of described step (4) is mixed by carvacrol, paeonol, eugenol, described Apium graveolens
Phenol, paeonol, the mass ratio of eugenol are 1:1:1.
Embodiment 2
Substantially the same manner as Example 1, differ only in: the antistaling agent in described step (4) is by Cortex Moutan
Phenol, eugenol 1:1 in mass ratio is uniformly mixed and obtains.Can be prepared by the yeast β-Portugal of embodiment 2
Polysaccharide.
Embodiment 3
Substantially the same manner as Example 1, differ only in: the antistaling agent in described step (4) is by Apium graveolens
Phenol, eugenol 1:1 in mass ratio is uniformly mixed and obtains.Can be prepared by the yeast β-Portugal of embodiment 3
Polysaccharide.
Embodiment 4
Substantially the same manner as Example 1, differ only in: the antistaling agent in described step (4) is by Apium graveolens
Phenol, paeonol 1:1 in mass ratio is uniformly mixed and obtains.Can be prepared by the yeast β-Portugal of embodiment 4
Polysaccharide.
Embodiment 5
Substantially the same manner as Example 1, differ only in: the acid solution in described step (2) is by following matter
The raw material composition of amount percentage ratio: succinic acid 1.2%, citric acid 1.2%, distilled water 97.6%.By succinic acid,
Citric acid joins to be uniformly mixed in distilled water and i.e. obtains acid solution.Yeast β-the Portugal obtaining embodiment 5 gathers
Sugar.
Embodiment 6
Substantially the same manner as Example 1, differ only in: the acid solution in described step (2) is by following matter
The raw material composition of amount percentage ratio: tartaric acid 1.2%, citric acid 1.2%, distilled water 97.6%.By tartaric acid,
Citric acid joins to be uniformly mixed in distilled water and i.e. obtains acid solution.Yeast β-the Portugal obtaining embodiment 6 gathers
Sugar.
Embodiment 7
Substantially the same manner as Example 1, differ only in: the acid solution in described step (2) is by following matter
The raw material composition of amount percentage ratio: tartaric acid 1.2%, succinic acid 1.2%, distilled water 97.6%.By tartaric acid,
Succinic acid joins to be uniformly mixed in distilled water and i.e. obtains acid solution.Yeast β-the Portugal obtaining embodiment 7 gathers
Sugar.
Test case 1
The productivity of the yeast beta-dextran preparing embodiment 1-7 is tested.
Weigh 10mg sample in tool plug test tube, add the sulfuric acid solution of 1.5mL 72% (m/m), mixed
Even, room temperature places 3h, is subsequently adding deionized water and makes the ultimate density of sulphuric acid be 2mol/L, in 100 DEG C
Lower hydrolysis 4h, takes out and is cooled to room temperature, adjusts pH the most neutral (6.5～7.0), then uses 0.2mol/L phosphorus
Acid buffer (pH7.0) is settled to 100mL, takes 2mL (blank takes 2mL water) and is placed in 10mL appearance
In measuring bottle, add the double enzyme reagent of 3mL GOPGD, 37 DEG C of reaction 1h, add water and be settled to 10mL, purple
Outer spectrophotometric determination light absorption value, calculates sample beta-dextran content.
Productivity (%)=yeast beta-dextran quality/saccharomyces cerevisiae quality × 100
Concrete outcome is shown in Table 1.
Table 1: yeast beta-dextran yield results list position: %
| Yeast beta-dextran productivity | |
| Embodiment 1 | 26.2 |
| Embodiment 2 | 21.5 |
| Embodiment 3 | 20.7 |
| Embodiment 4 | 21.2 |
| Embodiment 5 | 22.6 |
| Embodiment 6 | 22.4 |
| Embodiment 7 | 21.5 |
Comparing embodiment 1 and embodiment 2-4, embodiment 1 (carvacrol, paeonol, eugenol are compounding)
Yeast beta-dextran productivity is apparently higher than embodiment 2-4 (in carvacrol, paeonol, eugenol any two
Person compounds)；Comparing embodiment 1 and embodiment 5-7, embodiment 1 (tartaric acid, succinic acid, citric acid
Compounding) yeast beta-dextran productivity is apparently higher than embodiment 5-7 (in tartaric acid, succinic acid, citric acid
Arbitrarily the two compounds).
Test case 2
Yeast beta-dextran embodiment 1-7 prepared, is placed in 25 DEG C, protects under relative humidity 85% environment
Hide half a year, use " GB/T 4789.2-2010 microbiological test of food hygiene total plate count mensuration "
Carry out total plate count test.Concrete test result is shown in Table 2.
Table 2: total plate count test table cfu/g
Inventor is found surprisingly that, comparing embodiment 1 and embodiment 2-4, embodiment 1 (carvacrol, pellet
Skin phenol, eugenol are compounding) antiseptic property is substantially better than embodiment 2-4 (carvacrol, paeonol, Flos Caryophylli
In phenol, arbitrarily the two is compounding)；Comparing embodiment 1 and embodiment 5-7, embodiment 1 (tartaric acid, succinum
Acid, citric acid compound) antiseptic property is substantially better than embodiment 5-7 (tartaric acid, succinic acid, citric acid
In arbitrarily the two is compounding).
Test case 3
Yeast beta-dextran embodiment 1-7 prepared carries out DPPH free radical scavenging activity test.
DPPH method is a kind of high method measuring antioxidant activity quick, easy, repeated.Weigh
DPPH powder 0.0039g, with anhydrous alcohol solution DPPH powder, and is settled to 100mL, is configured to
Ultimate density is the DPPH ethanol solution of 0.1mmol/L.Yeast β-Portugal embodiment 1-6 prepared gathers
Sugar is configured to the sample of same concentrations, is mixed with the DPPH ethanol solution of 2.0mL by the sample of 2.0ml
Uniformly, at 25 DEG C after lucifuge reaction 1h, measure at light absorption value As first for 517nm；Meanwhile, water is used
Measure light absorption value as a control group with the reaction of DPPH ethanol solution and be labeled as Ac, with sample and not containing
The ethanol solution reaction of DPPH measures light absorption value as background group and is labeled as Ax.
DPPH free radical scavenging activity (%)=100-(As-Ax)/Ac × 100
Test result is shown in Table 3.
Table 3:DPPH free radical scavenging activity test result list position: %
| DPPH free radical scavenging activity | |
| Embodiment 1 | 29 |
| Embodiment 2 | 25 |
| Embodiment 3 | 24 |
| Embodiment 4 | 22 |
| Embodiment 5 | 23 |
| Embodiment 6 | 21 |
| Embodiment 7 | 22 |
In comparing embodiment 1 and embodiment 2-4, embodiment 1 (carvacrol, paeonol, eugenol are compounding)
DPPH free radical scavenging activity is apparently higher than embodiment 2-4 (in carvacrol, paeonol, eugenol arbitrarily
The two is compounding)；Comparing embodiment 1 and embodiment 5-7, embodiment 1 (tartaric acid, succinic acid, Fructus Citri Limoniae
Acid is compounding) DPPH free radical scavenging activity is apparently higher than embodiment 5-7 (tartaric acid, succinic acid, Fructus Citri Limoniae
In acid, arbitrarily the two is compounding).
Claims (2)
1. the preparation method of a yeast beta-dextran, it is characterised in that comprise the following steps:
(1) being added by the saccharomyces cerevisiae of 8-12 weight portion in the enzyme liquid of 35-85 weight portion, regulation pH is extremely
5-10, induces self-dissolving 12-28 hour under 48-62 DEG C of water-bath, and enzyme denaturing is lived, obtained enzymolysis solution；
(2) enzymolysis solution is centrifuged, washing, is precipitated thing, precipitate is joined 12-28 weight portion
Acid solution in, acidolysis 1-5 hour at 70-120 DEG C, centrifugal, washing, obtain acidolysis thing；
(3) by acidolysis thing washing with alcohol 1-3 time, filter, obtain filtering residue；
(4) by filtering residue with antistaling agent it is 100:(0.01-0.1 in mass ratio) mix, obtain mixed powder,
Dry, to obtain final product；
Enzyme liquid in described step (1) is made up of the raw material of following mass percent: papain
1-15%, water 87-97%.
The antistaling agent of described step (4) is mixed by carvacrol, paeonol, eugenol, described Apium graveolens
Phenol, paeonol, the mass ratio of eugenol are (1-3): (1-3): (1-3).
Acid solution in described step (2) is made up of the raw material of following mass percent: tartaric acid 0.2-2%,
Succinic acid 0.2-2%, citric acid 0.2-2%, water 94-99%.
2. the preparation method of yeast beta-dextran as claimed in claim 1, it is characterised in that: described
The flame filter press that is filtered in step (3) filters, and uses the filter cloth of 150-200 mesh.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610414670.7A CN106008741A (en) | 2016-06-14 | 2016-06-14 | Preparation method of yeast beta-glucan |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610414670.7A CN106008741A (en) | 2016-06-14 | 2016-06-14 | Preparation method of yeast beta-glucan |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106008741A true CN106008741A (en) | 2016-10-12 |
Family
ID=57087976
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610414670.7A Pending CN106008741A (en) | 2016-06-14 | 2016-06-14 | Preparation method of yeast beta-glucan |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106008741A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1192247A (en) * | 1995-07-05 | 1998-09-02 | 卡尔顿和联合酿酒有限公司 | Production of 'beta'-glucan-mannan preparations by autolysis of cells under certain pH, temperature and time conditions |
| CN101897431A (en) * | 2010-06-21 | 2010-12-01 | 中国农业大学 | Method for simultaneously preparing yeast extract and beta-1,3-glucan |
-
2016
- 2016-06-14 CN CN201610414670.7A patent/CN106008741A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1192247A (en) * | 1995-07-05 | 1998-09-02 | 卡尔顿和联合酿酒有限公司 | Production of 'beta'-glucan-mannan preparations by autolysis of cells under certain pH, temperature and time conditions |
| CN101897431A (en) * | 2010-06-21 | 2010-12-01 | 中国农业大学 | Method for simultaneously preparing yeast extract and beta-1,3-glucan |
Non-Patent Citations (1)
| Title |
|---|
| 赵光远等: "用废啤酒酵母制备碱不溶性葡聚糖的研究", 《微生物学通报》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2910287C (en) | Sporoderm-breaking method for ganoderma spore powder and products obtained by the same | |
| CN108004282B (en) | Method for preparing lucid ganoderma sporocarp fermentation product, fermentation product and application thereof | |
| CN106117387B (en) | A kind of low molecular weight tremella polysaccharides and the preparation method and application thereof | |
| CN102240259B (en) | Cicada extract shampoo and preparation thereof | |
| CN102242044B (en) | Cordyceps cicadae wine and preparation method thereof | |
| CN105754772B (en) | A kind of quinoa fruit wine and preparation method thereof | |
| KR101467903B1 (en) | The preparing method of immune improving agents | |
| CN110151813B (en) | Codonopsis pilosula extract and preparation method and application thereof | |
| CN104351752B (en) | It is a kind of from edible mushroom marine alga GL-PP compound functional capsule product production method | |
| CN113476380A (en) | Preparation method of houttuynia cordata rice fermentation product filtrate composition | |
| CN107114758A (en) | A kind of ferment cordyceps sinensis D-mannitol powder and preparation method thereof | |
| CN107217082A (en) | A kind of deer hemalbumin polypeptide and application | |
| CN102524799A (en) | Protein powder for improving organism immunity and preparation method thereof | |
| US10190142B2 (en) | High-purity galactooligosaccharide compositions, preparations, and applications thereof | |
| CN113912750B (en) | Method for extracting ganoderma lucidum fruiting body polysaccharide through fermentation pretreatment | |
| CN107177655A (en) | A kind of protein of folium mori polypeptide and application | |
| JP4409488B2 (en) | Method for producing chlorella fermented food | |
| CN105925640B (en) | The preparation method of yeast beta-dextran | |
| CN104211829B (en) | Preparation and application of white fungus functional polysaccharide | |
| CN106008741A (en) | Preparation method of yeast beta-glucan | |
| CN106821935A (en) | The method that bacteria inhibiting composition prepares cosmetics is extracted from TAL waste material | |
| CN114533628B (en) | Red tassel seed sorghum cereal fermentation product, external skin preparation containing same and preparation method and application thereof | |
| CN108641005B (en) | Preparation method and application of sulfated echinacea polysaccharide | |
| US20220370538A1 (en) | Method for extracting functional ingredients of mulberry leaves using enzymes | |
| CN107988024A (en) | Health care Noni fruit wine |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20161012 |
|
| RJ01 | Rejection of invention patent application after publication |