CN106000490B - Any linear or conic section profile Concentration gradient generator can be produced - Google Patents
Any linear or conic section profile Concentration gradient generator can be produced Download PDFInfo
- Publication number
- CN106000490B CN106000490B CN201610594276.6A CN201610594276A CN106000490B CN 106000490 B CN106000490 B CN 106000490B CN 201610594276 A CN201610594276 A CN 201610594276A CN 106000490 B CN106000490 B CN 106000490B
- Authority
- CN
- China
- Prior art keywords
- interconnection
- vertical channel
- vertical
- distance
- port
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0694—Creating chemical gradients in a fluid
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/12—Specific details about manufacturing devices
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Dispersion Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Catching Or Destruction (AREA)
- Branch Pipes, Bends, And The Like (AREA)
Abstract
Any linear and conic section profile Concentration gradient generator can be produced the invention discloses a kind of, is simple pipeline jointing construction, including during 2 arrival ends, be corresponding with 4 layers of branched bottom；During including 3 arrival ends, the corresponding branched bottom that haves three layers, every layer of branched bottom is connected by interconnection with Vertical Channel respectively, and the internal diameter size requirement of pipeline is in 0.1mm 0.2mm.The present invention realizes the controllability of concentration gradient shape, it can realize that the concentration gradient shape in 2 arrival end Concentration gradient generator exits changes at an angle using the flow velocity for adjusting solution, the peak value of the concentration gradient shape in 3 arrival end Concentration gradient generator exits is in certain density change.The present invention is of great significance the taxis that cell is studied in bioengineering and chemical engineering.
Description
Technical field
The invention belongs to micro fluidic chip technical field, and in particular to one kind can produce any linear or conic section profile
Concentration gradient generator.
Background technology
Micro-fluidic chip be it is a kind of interact integrated micro-total analysis system, micro-fluidic chip oneself moved towards through starting from laboratory
Using the research to micro-fluidic chip is of great significance with industrialization.Concentration gradient generator be to micro-fluidic chip into
One step application.Organism can experience a variety of chemical molecular signals in surrounding environment, and change itself according to chemical concentration gradients
Physiological activity.Using micro-fluidic chip environmental test, establish the concentration gradient that different organisms researchs need, this for
The fields such as drug screening, biotechnology, environment measuring have the function that important.
The contour shape that flow velocity difference of the liquid in microchannel may result in the concentration gradient that its outlet produces differs,
This phenomenon can have an impact the taxis for studying cell.Concentration gradient generator be using its produce different concentration gradient come
Study the taxis of cell.In microchip, the flow velocity of liquid is an important factor for influencing concentration-gradient curve shape.If liquid
The flow velocity grasp of body is bad, is difficult to form expected concentration gradient.Before, few people are to produced by different fluid velocities
Various concentrations gradient carry out system research.There is presently no a kind of offer for being capable of system in lower generation not similar shape different in flow rate
The Concentration gradient generator of the concentration gradient of shape so that expected concentration gradient is realized by coutroi velocity.
The content of the invention
The purpose of the present invention is overcome above-mentioned shortcoming existing in the prior art, there is provided one kind can produce arbitrarily linearly or two
The Concentration gradient generator of secondary curved profile, in 2 arrival ends and the Concentration gradient generator of 3 arrival ends provided,
Concentration gradient of different shapes is obtained by controlling the flow velocity of solution.
For this reason, any linear or conic section profile Concentration gradient generator can be produced the present invention provides a kind of, its
Technical solution is：Any linear or conic section profile Concentration gradient generator, including 2 or 3 arrival ends can be produced, when
During including 2 arrival ends, the conduit wall of 2 arrival ends through the first interconnection is connected with the first interconnection, described
First interconnection is connected with the upper port of 3 the first Vertical Channels, and 3 first Vertical Channels are suitable by from left to right
Sequence equidistantly distributed, and connect positioned at the first Vertical Channel of high order end with the left end of the first interconnection, positioned at low order end
The first Vertical Channel connect with the right end of the first interconnection；The lower port of 3 article of first Vertical Channel is respectively with
Two interconnections are connected, and second interconnection is connected with the upper port of 4 the second Vertical Channels, described 4 second
Vertical Channel is by order equidistantly distributed from left to right, and the second Vertical Channel positioned at high order end and the second interconnection
Left end connects, and the second Vertical Channel positioned at low order end connects with the right end of the second interconnection；Described 4 second vertical
The lower port of passage is connected with the 3rd interconnection respectively, the upper end of the 3rd interconnection and 5 article of the 3rd Vertical Channel
Mouth is connected, and 5 article of the 3rd Vertical Channel is by order equidistantly distributed from left to right, and the 3rd positioned at high order end is vertical
Passage connects with the left end of the 3rd interconnection, positioned at the 3rd Vertical Channel and the right end of the 3rd interconnection of low order end
Connect；The lower port of 5 article of the 3rd Vertical Channel is connected with the 4th interconnection respectively, the 4th interconnection and 6
The upper port of article the 4th Vertical Channel is connected, 6 article of the 4th Vertical Channel by order equidistantly distributed from left to right, and
The 4th Vertical Channel positioned at high order end connects with the left end of the 4th interconnection, positioned at low order end the 4th Vertical Channel with
The right end of 4th interconnection connects；The lower port of 6 article of the 4th Vertical Channel is gathered together, and is connected with the port of export
It is logical；When including 3 arrival ends, the conduit wall of 3 arrival ends through the first interconnection is connected with the first interconnection,
First interconnection is connected with the upper port of 4 the first Vertical Channels, and 4 first Vertical Channels are by from left to right
Order equidistantly distributed, and connect positioned at the first Vertical Channel of high order end with the left end of the first interconnection, positioned at most
First Vertical Channel of right end connects with the right end of the first interconnection (2)；The lower port of 4 first Vertical Channels point
It is not connected with the second interconnection, second interconnection is connected with the upper port of 5 the second Vertical Channels, and described 5
The second Vertical Channel of bar is by order equidistantly distributed from left to right, and the second Vertical Channel and second positioned at high order end is laterally
The left end of passage connects, and the second Vertical Channel positioned at low order end connects with the right end of the second interconnection；Described 5 article
The lower port of two Vertical Channels is connected with the 3rd interconnection respectively, the 3rd interconnection and 6 article of the 3rd Vertical Channel
Upper port be connected, 6 article of the 3rd Vertical Channel is by order equidistantly distributed from left to right, and positioned at the of high order end
Three Vertical Channels connect with the left end of the 3rd interconnection, positioned at the 3rd Vertical Channel and the 3rd interconnection of low order end
Right end connects；The lower port of 6 article of the 3rd Vertical Channel is gathered together, and is connected with the port of export.
Preferably, each arrival end is that the length being connected with the first interconnection is the vertical logical of 0.3mm-3cm
Road, the size of two entrances end passage are identical；Each arrival end is by order distribution from left to right, the wherein arrival end of high order end
The distance between left end of center line and the first interconnection is equal to the center line of the arrival end of low order end and first and laterally leads to
The distance between the right end in road；When the quantity of arrival end is 3, positioned at arrival end and the right side in middle arrival end and left side
The distance between arrival end of side is equal；When the quantity of arrival end is 2, two the first adjacent Vertical Channel center lines it
Between distance, the distance between two the second adjacent Vertical Channel center lines, two the 3rd adjacent Vertical Channel center lines
The distance between and the distance between two the 4th adjacent Vertical Channel center lines be equal；When the quantity of arrival end is 3
When a, between the distance between two first adjacent Vertical Channel center lines, two the second adjacent Vertical Channel center lines
Distance and the distance between two the 3rd adjacent Vertical Channel center lines be equal.
Preferably, the specific length of each arrival end is 0.5mm；The specific internal diameter of each arrival end is 0.2mm；
The distance between each two adjacent entries end center line is 1.4mm；The wherein center line of the arrival end of high order end and the first transverse direction
The distance between left end of passage is 0.8mm；The distance between the two described first adjacent Vertical Channel center lines, two
The distance between the distance between a second adjacent Vertical Channel center line, two the 3rd adjacent Vertical Channel center lines with
And the distance between two the 4th adjacent Vertical Channel center lines are 1.4mm；Wherein the first Vertical Channel of high order end
The distance between center line and the second interconnection left end, the center line of the first Vertical Channel of low order end and second laterally logical
Between the distance between road right end, the center line of the second Vertical Channel of high order end and the 3rd interconnection left end away from
From the distance between the center line of the second Vertical Channel of, low order end and the 3rd interconnection right end, high order end it is the 3rd perpendicular
The distance between the center line of straight channel and the 4th interconnection left end, low order end the 3rd Vertical Channel center line and
The distance between four interconnection right ends are equal, and are 0.8mm.
It is preferred that when the quantity of arrival end is 2, the lower ports of described 6 articles of the 4th Vertical Channels be with not overlapping,
The Uncrossed mode parallel remittance port of export；When the quantity of arrival end is 2, described 6 articles of the 3rd Vertical Channels
Lower port be the Uncrossed mode parallel remittance port of export with not overlapping.
It is preferred that the port of export is a vertical passageway, the internal diameter 1.2mm of the port of export；When the quantity of arrival end is 2
When, the passage length of the port of export is 0.5mm, and when the quantity of arrival end is 3, the passage length of the port of export is 2mm.
Preferably, the arrival end, the port of export, the first Vertical Channel, the second Vertical Channel, the 3rd Vertical Channel, the 4th
Vertical Channel, the first interconnection, the second interconnection, the internal diameter of the 3rd interconnection and the 4th interconnection are
0.1mm-0.2mm；First Vertical Channel, the second Vertical Channel, the 3rd Vertical Channel, the 4th Vertical Channel, first are laterally
Passage, the second interconnection, the 3rd interconnection and the 4th interconnection are equal in length, the length of 3-6cm.
Preferably, the arrival end, the port of export, the first Vertical Channel, the second Vertical Channel, the 3rd Vertical Channel, the 4th
Vertical Channel, the first interconnection, the second interconnection, the internal diameter of the 3rd interconnection and the 4th interconnection are
0.2mm。
Beneficial effects of the present invention：The microchannel graphics shape such as " Christmas tree " shape structure of the present invention.The present invention is mainly with 2
Based on a arrival end and 3 arrival end microchannels.By injecting the liquid of various concentrations, the speed of liquid is controlled, to distinguish
Obtain any linear and arbitrary curve Concentration gradient generator.The present invention realizes the reliability of concentration gradient shape, can profit
Realize that the shape of 2 arrival end linear concentration gradients changes at an angle with the flow velocity for adjusting solution, 3 arrival end curves
The peak value of concentration gradient shape is in certain density change.This to research bioengineering and chemical engineering in cell trend research
It is of great significance.
The present invention is described in further details below with reference to attached drawing.
Brief description of the drawings
1st, Fig. 1 is the structure diagram of the present invention；Wherein Fig. 1 (a) is 2 arrival ends；Fig. 1 (b) is 3 arrival ends；Figure
In all dimensional units be mm (millimeter)；
2nd, Fig. 2 is the solution concentration figure in passage, and white line L represents what concentration gradient was produced in the port of export at figure middle outlet end
Face；
3rd, Fig. 3 (a) is 2 arrival end concentration gradient linear graphs；Fig. 3 (b) is 3 arrival end concentration gradient conic sections
Figure.
Description of reference numerals：1st, arrival end；2nd, the first interconnection；3rd, the first Vertical Channel；4th, the second interconnection；5、
Second Vertical Channel；6th, the 3rd interconnection；7th, the 3rd Vertical Channel；8th, four interconnection；9th, the 4th Vertical Channel；10th, go out
Mouth end.
Embodiment
Below in conjunction with the accompanying drawings, the specific embodiment of the present invention is described in detail, it is to be understood that of the invention
Protection domain and from the limitation of embodiment.
As shown in Figure 1, any linear or conic section profile concentration gradient generation can be produced the present invention provides a kind of
Device, including 2 or 3 arrival ends 1, as shown in Fig. 1 (a), when including 2 arrival ends 1,2 arrival ends 1 are horizontal through first
It is connected to the conduit wall of passage 2 with the first interconnection 2, first interconnection 2 is upper with 3 the first Vertical Channels 3
Port is connected, and 3 first Vertical Channels 3 are located at the first of high order end by order equidistantly distributed from left to right
Vertical Channel 3 connects with the left end of the first interconnection 2, positioned at the first Vertical Channel 3 and the first interconnection 2 of low order end
Right end connect；The lower port of 3 first Vertical Channels 3 is connected with the second interconnection 4 respectively, and described second is horizontal
It is connected to passage 4 with the upper port of 4 the second Vertical Channels 5,4 second Vertical Channels 5 are by order from left to right
Equidistantly distributed, and connect positioned at the second Vertical Channel 5 of high order end with the left end of the second interconnection 4, positioned at low order end
The second Vertical Channel 5 connect with the right end of the second interconnection 4；The lower port of 4 second Vertical Channels 5 respectively with
3rd interconnection 6 is connected, and the 3rd interconnection 6 is connected with the upper port of 5 article of the 3rd Vertical Channel 7, described 5
3rd Vertical Channel 7 is by order equidistantly distributed from left to right, and the 3rd Vertical Channel 7 and the 3rd for being located at high order end is horizontal
The left end of passage 6 connects, and the 3rd Vertical Channel 7 positioned at low order end connects with the right end of the 3rd interconnection 6；Described 5
The lower port of article the 3rd Vertical Channel 7 is connected with the 4th interconnection 8 respectively, the 4th interconnection 8 and 6 article the 4th it is perpendicular
The upper port of straight channel 9 is connected, and 6 article of the 4th Vertical Channel 9 is located at most by order equidistantly distributed from left to right
4th Vertical Channel 9 of left end connects with the left end of the 4th interconnection 8, positioned at the 4th Vertical Channel 9 of low order end and
The right end of four interconnections 8 connects；The lower port of 6 article of the 4th Vertical Channel 9 is gathered together, and is connected with the port of export 10
It is logical；As shown in Fig. 1 (b), when including 3 arrival ends 1, conduit wall and of 3 arrival ends 1 through the first interconnection 2
One interconnection 2 is connected, and first interconnection 2 is connected with the upper port of 4 the first Vertical Channels 3, described 4 article
One Vertical Channel 3 is laterally led to by order equidistantly distributed from left to right, and positioned at the first Vertical Channel 3 and first of high order end
The left end in road 2 connects, and the first Vertical Channel 3 positioned at low order end connects with the right end of the first interconnection 2；Described 4
The lower port of first Vertical Channel 3 is connected with the second interconnection 4 respectively, second interconnection 4 and 5 it is second vertical
The upper port of passage 5 is connected, and 5 second Vertical Channels 5 are located at most left by order equidistantly distributed from left to right
Second Vertical Channel 5 at end connects with the left end of the second interconnection 4, positioned at the second Vertical Channel 5 and second of low order end
The right end of interconnection 4 connects；The lower port of 5 article of second Vertical Channel 5 is connected with the 3rd interconnection 6 respectively,
3rd interconnection 6 is connected with the upper port of 6 article of the 3rd Vertical Channel 7, and 6 article of the 3rd Vertical Channel 7 is pressed from a left side
Connect to right order equidistantly distributed, and positioned at the 3rd Vertical Channel 7 of high order end with the left end of the 3rd interconnection 6,
The 3rd Vertical Channel 7 positioned at low order end connects with the right end of the 3rd interconnection 6；Under 6 article of the 3rd Vertical Channel 7
Trunking together, and is connected with the port of export 10.
Further, each arrival end 1 is the vertical of 0.3mm-3cm for the length being connected with the first interconnection 2
Passage, each arrival end 1 is by order distribution from left to right, the wherein center line of the arrival end 1 of high order end and the first interconnection 2
The distance between left end be equal between the center line of arrival end 1 of low order end and the right end of the first interconnection 2 away from
From；When the quantity of arrival end 1 is 3, between middle arrival end 1 and the arrival end 1 in left side and the arrival end 1 on right side
Distance it is equal；When the quantity of arrival end 1 is 2, the distance between two adjacent 3 center lines of the first Vertical Channel, two
The distance between the distance between a adjacent 5 center line of the second Vertical Channel, two adjacent 7 center lines of the 3rd Vertical Channel
And the distance between two adjacent 9 center lines of the 4th Vertical Channel are equal；When the quantity of arrival end 1 is 3, two
The distance between the distance between adjacent 3 center line of the first Vertical Channel, two adjacent 5 center lines of the second Vertical Channel with
And the distance between two adjacent 7 center lines of the 3rd Vertical Channel are equal.
Further, the specific length of each arrival end 1 is 0.5mm；Each the specific internal diameter of arrival end 1 is
0.2mm；The distance between 1 center line of each two adjacent entries end is 1.4mm；Wherein the center line of the arrival end 1 of high order end and
The distance between left end of first interconnection 2 is 0.8mm；Two adjacent 3 center lines of the first Vertical Channel it
Between distance, the distance between two adjacent 5 center lines of the second Vertical Channel, two adjacent 7 centers of the 3rd Vertical Channel
The distance between line and the distance between two adjacent 9 center lines of the 4th Vertical Channel are 1.4mm；Wherein high order end
The distance between 4 left end of center line and the second interconnection of first Vertical Channel 3, the first Vertical Channel 3 of low order end
The distance between 4 right end of center line and the second interconnection, the center line of the second Vertical Channel 5 of high order end and the 3rd are laterally
Between the distance between 6 left end of passage, 6 right end of center line and the 3rd interconnection of the second Vertical Channel 5 of low order end
Distance, the distance between 8 left end of center line and the 4th interconnection of the 3rd Vertical Channel 7 of high order end, low order end
The distance between the center line of 3rd Vertical Channel 7 and 8 right end of the 4th interconnection are equal, and are 0.8mm.It is above-mentioned all
The channel wall thickness 0.1mm of passage, therefore, for having the Concentration gradient generator at two entrances end, the first interconnection
Length is 3mm, and the Vertical Channel center line of all layers of leftmost side with a distance from adjacent interconnection left end is 0.8mm from below,
The length of second interconnection is 4.4mm, and the length of the 3rd interconnection is 5.8mm, and the length of the 4th interconnection is
7.2mm, and the port of export that the lower end of 6 Vertical Channels is gathered together is the parallel remittance port of export, it is not overlapping, do not intersect.And 3
The interconnection of each layer of arrival end and the corresponding size of top close to its Vertical Channel are identical.In the present embodiment I
In order to make the mixed effect in later stage more preferable, the exit passageway sizes of 3 entrance concentration gradient generators is internal diameter 1.2mm, length
2mm。
Further, when the quantity of arrival end 1 is 2, the lower port of described 6 articles of the 4th Vertical Channels 9 is with not
It is overlapping, the Uncrossed mode parallel remittance port of export 10；When the quantity of arrival end 1 is 3, described 6 article the 3rd
The lower port of Vertical Channel 7 is the Uncrossed mode parallel remittance port of export 10 with not overlapping.
Further, the port of export 10 is a vertical passageway, the internal diameter 1.2mm of the port of export 10；When the quantity of arrival end 1
For 2 when, the passage length of the port of export 10 is 0.5mm, when arrival end 1 quantity be 3 when, the passage length of the port of export 10 is
2mm。
Further, the arrival end 1, the port of export 10, the first Vertical Channel 3, the second Vertical Channel the 5, the 3rd are logical vertically
Road 7, the 4th Vertical Channel 9, the first interconnection 2, the second interconnection 4, the 3rd interconnection 6 and the 4th interconnection 8
Internal diameter be 0.1mm-0.2mm；First Vertical Channel 3, the second Vertical Channel 5, the 3rd Vertical Channel the 7, the 4th are vertical
Passage 9, the first interconnection 2, the second interconnection 4, the 3rd interconnection 6 and the 4th interconnection 8 are equal in length,
The length of 3-6cm.
Further, the arrival end 1, the port of export 10, the first Vertical Channel 3, the second Vertical Channel the 5, the 3rd are logical vertically
Road 7, the 4th Vertical Channel 9, the first interconnection 2, the second interconnection 4, the 3rd interconnection 6 and the 4th interconnection 8
Internal diameter be 0.2mm.
The present invention is simple pipeline connection, and 2 arrival ends have 4 layers of branched bottom, and 3 arrival ends have three layers branched bottom,
Every layer of branched bottom is connected by interconnection with Vertical Channel respectively.The size requirement of pipeline should in 0.1mm-0.2mm to be best,
Do not answer deviation too big, because the present invention is tested based on microchannel, if size is too big, will not anticipate for research
Justice.If the quantity increase of arrival end, injecting the different natures of strength of fluid of each arrival end can cause to be formed at the port of export
Concentration gradient profile have certain change.
Wherein Fig. 2 is the solution concentration figure in passage, and white line L represents that concentration gradient is produced in the port of export at figure middle outlet end
Face, distances of the white line L away from branched bottom convergent point of 2 arrival ends of the invention is 0.48mm, the white line L of 3 arrival ends away from
The distance of branched bottom convergent point is 1.9mm.
The present invention is that the liquid of various concentrations is filled with different syringes in practical applications, with conduit by syringe
Liquid is imported in microchannel, and the syringe in experiment is installed on syringe pump, adjusts flow using syringe pump to realize flow velocity
Change.Exit is connected with conduit, and mixed liquid is introduced in liquids recovery bottle.
Fig. 3 is 2 arrival end concentration gradient linear graphs and 3 arrival end concentration gradient conic section figures.Wherein Fig. 3 .a tables
Show 14 kinds of different in flow rate 2 arrival end concentration gradient Line Charts, as can be seen from the figure with the reduction of flow velocity, solution mixing
Effect it is more obvious, concentration gradient gets over unobvious, is finally reached and is thoroughly mixed, and the core of contour line is in certain in figure
Angle change, the angle at the interval between every line is 5 degree.Fig. 3 .b represent 16 kinds of different in flow rate 3 arrival end concentration gradients
Curve map, as can be seen from the figure as the reduction of flow velocity, the peak value of conic section also reduce therewith, the effect of solution mixing is just
More obvious, concentration gradient gets over unobvious, is finally reached and is thoroughly mixed.
Emulation experiment of the present invention mainly uses COMSOL Multiphysics simulation softwares.Nowier-stokes(N-S)
Equation and convective-diffusion equation are the theoretical foundation calculated, can be calculated using the concentration calculation formula at each branch point ending dense
Spend the profile of gradient.But this formula requires its speed too small, outlet end passageways should be maintained at and be not mixed.By to net
The refinement of unit lattice, to realize the accuracy of this emulation.
In conclusion this invention describes a kind of Concentration gradient generator.By controlling 2 arrival ends and 3 entrances
The flow velocity of solution realizes the controllability of concentration gradient profile at the port of export in the Concentration gradient generator of end.In corresponding flow velocity
Under, the Concentration gradient generator of 2 arrival ends can produce any linear concentration gradient, and the concentration gradient of 3 arrival ends produces
Device can produce the concentration gradient of any conic section.
Concentration gradient generator is the branch by solution, and concentration gradient is finally realized at the port of export.This experiment
Designed structure graph is classical " Christmas tree " structure graph.The structure that microchannel is carried out with " CAXA " mapping software is set
Meter.Each branched bottom is straight channel, and final six branched bottoms together import 1 outlet end passageways.Demonstrate,proved by emulation experiment
Understand the controllability of the arbitrary concentration gradient profile of Concentration gradient generator generation of 2 arrival ends and 3 arrival ends.To concentration
The correlative study of gradient generator, can be used for studying taxis of the cell in various concentrations sample.This concentration gradient production
Raw device is mainly used in bioengineering and chemical engineering.
The present invention is produced with the method and fluid dynamic and mass transport theory of finite element to design with estimated concentration gradient
Raw device.It is an advantage of the invention that：The controllability of concentration gradient shape is realized, can realize 2 using the flow velocity of solution is controlled
Arrival end concentration gradient shape changes at an angle, and the peak value of 3 arrival end concentration gradient shapes is in certain density change.
Trend research to cell in research bioengineering and chemical engineering is of great significance.The present invention is come real by adjusting flow velocity
Existing concentration gradient is in the change of certain rule, this is very helpful for later stage concentration gradient research.Originally it can compare and be
Influence of the observation flow velocity of system to concentration gradient.Meanwhile the number of entrance is different, injects the difference of strength of fluid, this is for going out
The concentration gradient shape of mouth can also have a great impact.The concentration gradient of 2 entrances and 3 entrances designed by the present invention produces
The shape of concentration gradient caused by device is had nothing in common with each other, the former is presented linearly, and conic is presented in the latter.
Disclosed above is only several specific embodiments of the present invention, and still, the embodiment of the present invention is not limited to this, is appointed
What what those skilled in the art can think change should all fall into protection scope of the present invention.
Claims (5)
1. any linear or conic section profile Concentration gradient generator can be produced, it is characterised in that there are 2 or 3 and enter
Mouth end (1), when including 2 arrival end (1), the conduit wall and first of 2 arrival ends (1) through the first interconnection (2)
Interconnection (2) is connected, and first interconnection (2) is connected with the upper port of 3 the first Vertical Channels (3), and described 3
The first Vertical Channel of bar (3) is by order equidistantly distributed from left to right, and the first Vertical Channel (3) positioned at high order end and the
The left end of one interconnection (2) connects, positioned at the first Vertical Channel (3) and the right end of the first interconnection (2) of low order end
Head connects；The lower port of 3 first Vertical Channels (3) is connected with the second interconnection (4) respectively, and described second laterally
Passage (4) is connected with the upper port of 4 the second Vertical Channels (5), and 4 second Vertical Channels (5) are pressed from left to right
Order equidistantly distributed, and connect positioned at the second Vertical Channel (5) of high order end with the left end of the second interconnection (4), position
Connect in the second Vertical Channel (5) of low order end with the right end of the second interconnection (4)；4 second Vertical Channels (5)
Lower port be connected respectively with the 3rd interconnection (6), the 3rd interconnection (6) and 5 article of the 3rd Vertical Channel (7)
Upper port is connected, and 5 article of the 3rd Vertical Channel (7) is located at high order end by order equidistantly distributed from left to right
3rd Vertical Channel (7) connects with the left end of the 3rd interconnection (6), positioned at the 3rd Vertical Channel (7) and the of low order end
The right end of three interconnections (6) connects；The lower port of 5 article of the 3rd Vertical Channel (7) respectively with the 4th interconnection (8)
It is connected, the 4th interconnection (8) is connected with the upper port of 6 article of the 4th Vertical Channel (9), and described 6 article the 4th vertical
Passage (9) is by order equidistantly distributed from left to right, and the 4th Vertical Channel (9) positioned at high order end and the 4th interconnection
(8) left end connects, and the 4th Vertical Channel (9) positioned at low order end connects with the right end of the 4th interconnection (8)；It is described
The lower port of 6 article of the 4th Vertical Channel (9) is gathered together, and is connected with the port of export (10)；
When including 3 arrival end (1), conduit wall and first of 3 arrival ends (1) through the first interconnection (2) are laterally
Passage (2) is connected, and first interconnection (2) is connected with the upper port of 4 the first Vertical Channels (3), described 4 article
One Vertical Channel (3) is by order equidistantly distributed from left to right, and the first Vertical Channel (3) positioned at high order end and the first horizontal stroke
Connect to the left end of passage (2), positioned at the first Vertical Channel (3) and the right end phase of the first interconnection (2) of low order end
Connect；The lower port of 4 first Vertical Channels (3) is connected with the second interconnection (4) respectively, second interconnection
(4) upper port with 5 the second Vertical Channels (5) is connected, and 5 second Vertical Channels (5) are by order from left to right
Equidistantly distributed, and connect positioned at the second Vertical Channel (5) of high order end with the left end of the second interconnection (4), positioned at most
The second Vertical Channel (5) of right end connects with the right end of the second interconnection (4)；Under 5 second Vertical Channels (5)
Port is connected with the 3rd interconnection (6) respectively, the upper end of the 3rd interconnection (6) and 6 article of the 3rd Vertical Channel (7)
Mouth is connected, and 6 article of the 3rd Vertical Channel (7) is located at the 3rd of high order end the by order equidistantly distributed from left to right
Vertical Channel (7) connects with the left end of the 3rd interconnection (6), horizontal positioned at the 3rd Vertical Channel (7) of low order end and the 3rd
Connect to the right end of passage (6)；The lower port of 6 article of the 3rd Vertical Channel (7) is gathered together, and with the port of export (10)
Connection；
When the quantity of arrival end (1) is 2, the lower port of described 6 article of the 4th Vertical Channel (9) is with not overlapping, is not handed over
The parallel remittance port of export (10) of the mode of fork；When the quantity of arrival end (1) is 3, described 6 article the 3rd vertical logical
The lower port in road (7) is the Uncrossed mode parallel remittance port of export (10) with not overlapping；
Wherein, each arrival end (1) is that the length being connected with the first interconnection (2) is the vertical logical of 0.3mm-3cm
Road；The specific internal diameter of each arrival end (1) is 0.2mm；The distance between each two adjacent entries end (1) center line is 1.4mm；
Wherein the distance between left end of the center line of the arrival end (1) of high order end and the first interconnection (2) is 0.8mm；It is described
The distance between two adjacent the first Vertical Channel (3) center lines, two adjacent the second Vertical Channel (5) center lines it
Between distance, the distance between two the 3rd adjacent Vertical Channel (7) center lines and two the 4th adjacent Vertical Channels
(9) the distance between center line is 1.4mm；Wherein the center line of the first Vertical Channel (3) of high order end and second laterally leads to
The distance between road (4) left end, the center line of the first Vertical Channel (3) of low order end and the second interconnection (4) right end
The distance between, the distance between the center line of the second Vertical Channel (5) of high order end and the 3rd interconnection (6) left end,
The distance between the center line of the second Vertical Channel (5) of low order end and the 3rd interconnection (6) right end, the 3rd of high order end the
The distance between the center line of Vertical Channel (7) and the 4th interconnection (8) left end, the 3rd Vertical Channel (7) of low order end
The distance between center line and the 4th interconnection (8) right end be equal, and be 0.8mm；The port of export (10) is one
Vertical passageway, the internal diameter 1.2mm of the port of export (10)；When the quantity of arrival end (1) is 2, the passage length of the port of export (10)
For 0.5mm, when the quantity of arrival end (1) is 3, the passage length of the port of export (10) is 2mm.
2. as claimed in claim 1 can produce any linear or conic section profile Concentration gradient generator, its feature exists
In each arrival end (1) is laterally led to by order distribution from left to right, the wherein center line of the arrival end (1) of high order end and first
The distance between the left end in road (2) is equal to the center line of arrival end (1) and the right end of the first interconnection (2) of low order end
The distance between；When the quantity of arrival end (1) is 3, arrival end (1) and the right side positioned at middle arrival end (1) with left side
The distance between the arrival end (1) of side is equal；When the quantity of arrival end (1) is 2, two the first adjacent Vertical Channels
(3) the distance between the distance between center line, two adjacent the second Vertical Channel (5) center lines, two the adjacent the 3rd
The distance between Vertical Channel (7) center line and the distance between two the 4th adjacent Vertical Channel (9) center lines are homogeneous
Deng；When the quantity of arrival end (1) is 3, the distance between two adjacent the first Vertical Channel (3) center lines, two phases
Between the distance between adjacent the second Vertical Channel (5) center line and two the 3rd adjacent Vertical Channel (7) center lines
Distance is equal.
3. as claimed in claim 2 can produce any linear or conic section profile Concentration gradient generator, its feature exists
In the specific length of each arrival end (1) is 0.5mm.
4. any linear or conic section profile Concentration gradient generator, its feature can be produced as claimed in claim 2 or claim 3
It is, the arrival end (1), the port of export (10), the first Vertical Channel (3), the second Vertical Channel (5), the 3rd Vertical Channel
(7), the 4th Vertical Channel (9), the first interconnection (2), the second interconnection (4), the 3rd interconnection (6) and the 4th are horizontal
Internal diameter to passage (8) is 0.1mm-0.2mm；First Vertical Channel (3), the second Vertical Channel (5), the 3rd are led to vertically
Road (7), the 4th Vertical Channel (9), the first interconnection (2), the second interconnection (4), the 3rd interconnection (6) and the 4th
Interconnection (8) are equal in length, the length of 3-6cm.
5. as claimed in claim 4 can produce any linear or conic section profile Concentration gradient generator, its feature exists
In, the arrival end (1), the port of export (10), the first Vertical Channel (3), the second Vertical Channel (5), the 3rd Vertical Channel (7),
4th Vertical Channel (9), the first interconnection (2), the second interconnection (4), the 3rd interconnection (6) and the 4th are laterally logical
The internal diameter in road (8) is 0.2mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610594276.6A CN106000490B (en) | 2016-07-26 | 2016-07-26 | Any linear or conic section profile Concentration gradient generator can be produced |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610594276.6A CN106000490B (en) | 2016-07-26 | 2016-07-26 | Any linear or conic section profile Concentration gradient generator can be produced |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106000490A CN106000490A (en) | 2016-10-12 |
| CN106000490B true CN106000490B (en) | 2018-04-13 |
Family
ID=57114606
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610594276.6A Expired - Fee Related CN106000490B (en) | 2016-07-26 | 2016-07-26 | Any linear or conic section profile Concentration gradient generator can be produced |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106000490B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107271706B (en) * | 2017-07-05 | 2018-11-06 | 大连理工大学 | A kind of micro-fluidic chip of real-time and precise control observation point dynamic Some Circulating Factors concentration |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2447703A2 (en) * | 2010-10-29 | 2012-05-02 | Sony Corporation | Dielectric cytometric apparatus and dielectric-cytometric cell sorting method |
| CN103033608A (en) * | 2011-10-10 | 2013-04-10 | 中山大学 | Integration micro-fluidic chip for immune analysis research and applications thereof |
| CN103801412A (en) * | 2012-11-08 | 2014-05-21 | 中山大学 | Integrated micro-fluidic chip used for fluorescence detection of enzyme catalysis product and application thereof |
| CN104928178A (en) * | 2015-06-11 | 2015-09-23 | 浙江大学 | Three inlet concentration gradient generator and power function concentration gradient generation method |
| CN105080627A (en) * | 2015-08-25 | 2015-11-25 | 辽宁中医药大学 | Integrated microfluidic chip for screening medicine and method for applying integrated microfluidic chip |
| CN205925736U (en) * | 2016-07-26 | 2017-02-08 | 辽宁工业大学 | Can produce concentration gradient generator arbitrary linear and quadratic curve profile |
-
2016
- 2016-07-26 CN CN201610594276.6A patent/CN106000490B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2447703A2 (en) * | 2010-10-29 | 2012-05-02 | Sony Corporation | Dielectric cytometric apparatus and dielectric-cytometric cell sorting method |
| CN103033608A (en) * | 2011-10-10 | 2013-04-10 | 中山大学 | Integration micro-fluidic chip for immune analysis research and applications thereof |
| CN103801412A (en) * | 2012-11-08 | 2014-05-21 | 中山大学 | Integrated micro-fluidic chip used for fluorescence detection of enzyme catalysis product and application thereof |
| CN104928178A (en) * | 2015-06-11 | 2015-09-23 | 浙江大学 | Three inlet concentration gradient generator and power function concentration gradient generation method |
| CN105080627A (en) * | 2015-08-25 | 2015-11-25 | 辽宁中医药大学 | Integrated microfluidic chip for screening medicine and method for applying integrated microfluidic chip |
| CN205925736U (en) * | 2016-07-26 | 2017-02-08 | 辽宁工业大学 | Can produce concentration gradient generator arbitrary linear and quadratic curve profile |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106000490A (en) | 2016-10-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Sattari et al. | Multiphase flow in microfluidics: From droplets and bubbles to the encapsulated structures | |
| Glasgow et al. | Enhancement of microfluidic mixing using time pulsing | |
| Roudgar et al. | Numerical Study of Split T‐Micromixers | |
| CN105195053B (en) | A kind of concussion jetting type micro-mixer based on microbubble driving | |
| CN108144659A (en) | Based on chip lab microflow control technique micro-structure assisted extraction device and method | |
| CN106222088A (en) | A kind of for animal tissue's micro-fluidic chip that comparison is cultivated in situ | |
| CN109234163A (en) | A kind of high throughput tumor-targeting drug concentration screening micro-fluidic device | |
| CN106000490B (en) | Any linear or conic section profile Concentration gradient generator can be produced | |
| CN106268472A (en) | A kind of inverted V-shaped for passive micro-mixer multistage mixing array structure | |
| CN106943938A (en) | A kind of imitative vein channel design passive type micro-mixer | |
| CN207899465U (en) | Micro-fluidic chip, drop formation device and microballoon preparation facilities | |
| CN106669512B (en) | Passive type microchannel | |
| JP2004531369A (en) | Micro channel device | |
| CN106622415A (en) | concentration gradient micro-fluidic chip of three-dimensional internet structure | |
| CN103331121A (en) | Minitype fluid mixing system | |
| CN205925736U (en) | Can produce concentration gradient generator arbitrary linear and quadratic curve profile | |
| Wang et al. | A new bubble-driven pulse pressure actuator for micromixing enhancement | |
| CN105214546A (en) | A kind of concussion jetting type micro-mixer based on Pulsating Flow | |
| CN206519110U (en) | A kind of imitative vein channel design passive type micro-mixer | |
| CN106669513A (en) | Concentration gradient generating device and method for generating concentration gradient in one step | |
| Bedram et al. | Numerical investigation of droplets breakup in a microfluidic T-junction | |
| US20090323463A1 (en) | Devices And Fluid Flow Methods For Improving Mixing | |
| CN114931988A (en) | Nanoliter molecular concentration gradient droplet generation microfluidic chip and use method thereof | |
| CN104928178A (en) | Three inlet concentration gradient generator and power function concentration gradient generation method | |
| CN201371054Y (en) | Multifunctional micro-fluid mechanical system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180413 Termination date: 20180726 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |