CN105999403A - Bacterial cellulose membrane tissue-engineered artificial tendon scaffold mold - Google Patents
Bacterial cellulose membrane tissue-engineered artificial tendon scaffold mold Download PDFInfo
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- CN105999403A CN105999403A CN201610534326.1A CN201610534326A CN105999403A CN 105999403 A CN105999403 A CN 105999403A CN 201610534326 A CN201610534326 A CN 201610534326A CN 105999403 A CN105999403 A CN 105999403A
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- mold
- artificial tendon
- groove
- concrete
- lower mold
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/20—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/10—Materials or treatment for tissue regeneration for reconstruction of tendons or ligaments
Abstract
The invention relates to the technical field of tissue engineering materials, in particular to a bacterial cellulose membrane tissue-engineered artificial tendon scaffold mold. The mold is particularly formed by an upper mold body and a lower mold body which correspond to each other and are connected in a one-side mode through connecting hinges. The lower mold body is shaped like a box, the upper surface of the lower mold body is provided with a semi-columnar hollow mold groove, and an opening of the groove faces upwards; the peripheral rectangular edges of the mold groove are provided with wedge-shaped shearing blades higher than the upper surface. The upper mold body is shaped like a box and perpendicularly corresponds to the lower mold body in a mirror-image mode, the lower surface of the lower mold body is provided with a semi-columnar hollow mold groove, and an opening of the groove faces downwards; the peripheral rectangular edges of the mold groove are provided with wedge-shaped shearing blades higher than the lower surface. The bacterial cellulose membrane tissue-engineered artificial tendon scaffold mold serves as a specialized molding device for bacterial cellulose membrane (BC membrane) scaffold materials, has the advantages of being simple in structure, easy to operate, convenient to clean and the like and can be widely applied to molding of various scaffold materials.
Description
Technical field
The present invention relates to tissue engineering material technical field.In particular to a kind of bacteria cellulose film engineered artificial tendon scaffold mould.
Background technology
The impaired patient athlete often of tendon and be engaged in physical labourer, tendon could not be repaired and immobile situation in time, and limbs of patient and mental impression are painful, if abandoning cure, causes it disabled.In recent years, tissue engineering produced tendon is the method that clinical Tendon Defection reparation provided more preferably, met physiological characteristics, its cardinal principle is to provide a cell growth support that can meet tendon mechanical property, implant seed cell in vitro or in vivo, tendon sample tissue is formed by regulation and control, so that Tendon Defection is repaired, but the method requires technical difficulty bigger and preferable Tendon Tissue Engineering timbering material is very difficult to select.Preferably Tendon Tissue Engineering timbering material not only should possess the characteristic that the general timbering materials such as certain porosity, water absorption rate, good biocompatibility are had, and also should possess the characteristics such as corresponding mechanical strength, non-immunogenicity.
Bacterial cellulose (Bacterial
Cellulose BC) it is a kind of under natural environment, a kind of nano-grade cellulosic that fermentable produces.Formation of chemical bond is passed through by single glucose.Have good tensile resistance and toughness, excellent biocompatibility and biodegradability.Bacterial cellulose inherently fermentation generation in liquid environment, has good hydrophilic and retentiveness.Therefore, BC with before occur chemical industry composite fibre materials compare, mechanical property and biological affinity are all more nearly natural tendon, there is the potential becoming impaired tendon succedaneum.In tissue engineering research in recent years, BC film studies biomaterial as hot topic, achieves successfully in the lenticular replacement of eyes.So, it is preferably to select using BC film as the timbering material of tissue engineering produced tendon.
Fujian Normal University Huang Zhen, Huang Zuxin et al. have succeeded in developing " preparation method of the engineered artificial tendon scaffold of a kind of bacteria cellulose film " and have applied for patent of invention (applying on the same day with the present invention).This patent employing xylose Acetobacter gluconicum (Glucoacetobacter
xylinus), first prepare bacteria cellulose film (BC film), re-compacted be shaped into engineered artificial tendon scaffold.The requirement that the technical specifications such as prepared brace aperture rate, retentiveness, rehydration, mechanical property, cytotoxicity and cell compatibility meet, can be applicable to the preparation of engineered artificial tendon.
Either chemical industry composite fibre materials or Bacterial cellulose membrane material, is required for a kind of special former, has and meet porosity, retentiveness, rehydration, mechanical property, cell attachment rate and the rate of growth using requirement after guarantee material molding.
Summary of the invention
Present invention aim to provide a kind of and bacteria cellulose film can be suppressed the artificial tendon scaffold mould that qualitative one-tenth meets porosity, retentiveness, rehydration, mechanical property, cell attachment rate and the rate of growth of use requirement.This mould has a simple in construction simultaneously, the feature such as easy to use.
Use technical scheme as follows for realizing the purpose of the present invention:
Mould of the present invention, is made up of upper and lower two corresponding molds, and is connected by connecting hinge one side.
Described lower mold is concrete, and profile is box-like, and upper surface is provided with half-terete hollow mould groove, and notch is upwards；The surrounding rectangular edges edge of mold slots, is provided with the wedge shear-blade higher than upper surface.
Described upper mold is concrete, and profile is box-like, and vertical mirror concrete with lower mold is corresponding, and the lower surface that lower mold is concrete is provided with half-terete hollow mould groove, and notch is downward；The surrounding rectangular edges edge of mold slots, is provided with the wedge shear-blade higher than lower surface.
Upper and lower two corresponding molds are connected by being arranged at the unilateral hinge that connects, and conveniently close and open.When upper mold closes downwards, arranging wedge shear-blade in upper and lower two corresponding molds can correspondence coincide.The corresponding rear original half-terete hollow mould groove that coincide i.e. is transformed to columned hollow mould groove.When hollow mould groove is filled with timbering material, just can obtain columned timbering material entity.
The surface of described hollow mould groove, is provided with latticed annular knurl decorative pattern, and also there is the annular knurl decorative pattern of correspondence on the compressing artificial tendon scaffold surface being, is beneficial to cell attachment and growth.
Mould of the present invention, as the shaping equipment of bacteria cellulose film (BC film) timbering material, has simple in construction and easily operates, facilitates the advantages such as cleaning, can extensively apply and polytype timbering material molding.
Accompanying drawing explanation
Fig. 1 is the perspective view of artificial tendon scaffold mould.
Fig. 2 is the side view of the closure state of artificial tendon scaffold mould.
Detailed description of the invention:
In order to the present invention is better understood from, it is described further by way of example in conjunction with accompanying drawing.
In Fig. 1,1 is that upper mold is concrete；2 is the wedge shear-blade of upper mold concrete (1)；3 is concrete (1) the half-terete hollow mould groove of upper mold；4 is to connect hinge, upper mold concrete (1) is specifically connected with lower mold by this hinge；5 is the concrete half-terete hollow mould groove of lower mold；6 is the wedge shear-blade that lower mold is concrete；7 is that lower mold is concrete.
In Fig. 2,1 is that upper mold is concrete；2 is the wedge shear-blade of upper mold concrete (1)；3 is concrete (1) the half-terete hollow mould groove of upper mold；4 is to connect hinge, upper mold concrete (1) is specifically connected with lower mold by this hinge；5 is the concrete half-terete hollow mould groove of lower mold；6 is the wedge shear-blade that lower mold is concrete；7 is that lower mold is concrete.Described lower mold is concrete, and profile is box-like, and upper surface is provided with half-terete hollow mould groove, and notch is upwards；The surrounding rectangular edges edge of mold slots, is provided with the wedge shear-blade higher than upper surface.
Embodiment 1
According to present invention structure shown in the drawings, stainless steel material is used to be processed into mould.The size wherein going up hollow mold slots (3) and lower hollow mould groove (5) is: diameter: 50mm；Length 1000mm.
Before using, mould is carried out Integral cleaning and sterilizing, can use after air-drying.
During use, opening upper mold specifically (1), be filled in by bacteria cellulose film (BC film) in the half-terete hollow mould groove (5) in lower mold concrete (7), its loading is hollow mould groove (5)；Concrete for upper mold (1) is the most firmly compressed, now in the wedge shear-blade (2) in upper mold concrete (1) and lower mold specifically (7) wedge shear-blade (6) implant bacteria cellulose film (BC film) unnecessary part is neatly cut away；Open upper mold concrete (1), carefully take out implant, i.e. obtain described artificial tendon scaffold entity.
Owing to the surface configuration of hollow mould groove has latticed annular knurl decorative pattern, the surface of compressing artificial tendon scaffold entity also to have the annular knurl decorative pattern of correspondence.
Bacteria cellulose film (BC film) described in the present embodiment obtains in the following way: after xylose Acetobacter gluconicum (Glucoacetobacter xylinus) strain 1) is inoculated into the activation that solid medium carries out routine, the strain of activation is selected from solid medium, access the 500ml conical flask equipped with 100ml fluid medium, under conditions of 28 DEG C and 160r/min shaking table, cultivate 24～32h make xylose Acetobacter gluconicum seed liquor, standby；
2, xylose Acetobacter gluconicum seed liquor is with every liter of distiller grains degradation solution complex liquid access 6～the inoculum concentration of 8% volume, is linked in distiller grains degradation solution complex liquid, fully shakes, and after making bacterium solution uniformly, is positioned in 28 DEG C of incubators and carries out quiescent culture.After cultivating 4～6 days, it is seen that fluid medium liquid level is floating bacteria cellulose film, and now Bacterial cellulose film thickness is 2.5～4.5 mm；
3, taking-up floats on the bacteria cellulose film of media surface, after repeatedly rinsing with tap water, is put in the NaOH solution of 0.1M and heats and boil 10min, removes the impurity such as the culture medium in membrane removal and thalline, takes out, adds distilled water and rinse 10 min.Bacteria cellulose film it is put in the NaOH solution of 0.1M again and again boils 10min, repeatedly rinsing bacteria cellulose film with distilled water, till washings pH value is 7.0；
4) by bacteria cellulose film distillation water logging 24～28h, the bacteria cellulose film of white translucent is obtained；
Performance test
By BC film artificial tendon scaffold entity made above, carry out porosity, retentiveness, rehydration, Mechanics Performance Testing.The artificial tendon scaffold entity porosity that after measured prepared by the present invention is 93.5%；Retentiveness is 94.5%；Rehydration is 55, has at a relatively high hydrophilic, it is possible to the liquid environment being applicable in organism.
By artificial tendon scaffold entity made above, carry out ultimate tensile strength test.After tested, ultimate tensile strength is 48.8 N, elongation at break 16%.
It is I grade with the cytotoxicity of lixiviating solution method detection artificial tendon scaffold entity, BC film and artificial tendon scaffold entity cell growth avirulence is described, meets biomaterial safety requirements.
Claims (4)
1. a bacteria cellulose film engineered artificial tendon scaffold mould, is characterized in that described mould, is made up of upper and lower two corresponding molds, and is connected by connecting hinge one side.
A kind of bacteria cellulose film engineered artificial tendon scaffold mould the most according to claim 1, is characterized in that described lower mold is concrete, and profile is box-like, and upper surface is provided with half-terete hollow mould groove, and notch is upwards；The surrounding rectangular edges edge of mold slots, is provided with the wedge shear-blade higher than upper surface.
A kind of bacteria cellulose film engineered artificial tendon scaffold mould the most according to claim 1, it is characterized in that described upper mold is concrete, profile is box-like, and vertical mirror concrete with lower mold is corresponding, the lower surface that lower mold is concrete is provided with half-terete hollow mould groove, and notch is downward；The surrounding rectangular edges edge of mold slots, is provided with the wedge shear-blade higher than lower surface.
A kind of bacteria cellulose film engineered artificial tendon scaffold mould the most according to claim 1, is characterized in that the surface of described hollow mould groove, is provided with latticed annular knurl decorative pattern.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610534326.1A CN105999403A (en) | 2016-07-08 | 2016-07-08 | Bacterial cellulose membrane tissue-engineered artificial tendon scaffold mold |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610534326.1A CN105999403A (en) | 2016-07-08 | 2016-07-08 | Bacterial cellulose membrane tissue-engineered artificial tendon scaffold mold |
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| CN105999403A true CN105999403A (en) | 2016-10-12 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610534326.1A Pending CN105999403A (en) | 2016-07-08 | 2016-07-08 | Bacterial cellulose membrane tissue-engineered artificial tendon scaffold mold |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103921328A (en) * | 2014-04-16 | 2014-07-16 | 宁波瑞元模塑有限公司 | Peach wood decorative piece hot-pressing forming mold with automatic edge cutting function |
| CN205130241U (en) * | 2015-09-24 | 2016-04-06 | 钟正明 | Be applied to solid elastic ball's foaming forming die |
-
2016
- 2016-07-08 CN CN201610534326.1A patent/CN105999403A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103921328A (en) * | 2014-04-16 | 2014-07-16 | 宁波瑞元模塑有限公司 | Peach wood decorative piece hot-pressing forming mold with automatic edge cutting function |
| CN205130241U (en) * | 2015-09-24 | 2016-04-06 | 钟正明 | Be applied to solid elastic ball's foaming forming die |
Non-Patent Citations (1)
| Title |
|---|
| 顾其胜 等主编: "《实用生物医用材料学》", 30 September 2005, 上海：上海科学技术出版社 * |
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