CN105999220B - Preparation method of enramycin premix - Google Patents
Preparation method of enramycin premix Download PDFInfo
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- CN105999220B CN105999220B CN201610505031.1A CN201610505031A CN105999220B CN 105999220 B CN105999220 B CN 105999220B CN 201610505031 A CN201610505031 A CN 201610505031A CN 105999220 B CN105999220 B CN 105999220B
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- enramycin
- mycelium
- premix
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/04—Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
- A61K38/10—Peptides having 12 to 20 amino acids
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/141—Intimate drug-carrier mixtures characterised by the carrier, e.g. ordered mixtures, adsorbates, solid solutions, eutectica, co-dried, co-solubilised, co-kneaded, co-milled, co-ground products, co-precipitates, co-evaporates, co-extrudates, co-melts; Drug nanoparticles with adsorbed surface modifiers
- A61K9/145—Intimate drug-carrier mixtures characterised by the carrier, e.g. ordered mixtures, adsorbates, solid solutions, eutectica, co-dried, co-solubilised, co-kneaded, co-milled, co-ground products, co-precipitates, co-evaporates, co-extrudates, co-melts; Drug nanoparticles with adsorbed surface modifiers with organic compounds
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Immunology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
Abstract
The invention relates to the field of feed drug processing, in particular to a preparation method of enramycin premix, which comprises the following steps: carrying out plate-frame filtration on enramycin fermentation liquor; flash drying the obtained filter cake to obtain the mycelium of enramycin: diluting mycelium and auxiliary materials in proportion, and fully stirring: uniformly stirring the enramycin diluent, and then drying to obtain enramycin premix; the weight ratio of the enramycin mycelium to the auxiliary materials is 4:6; the added auxiliary materials are glycerides, the degradation degree of finished enramycin premix particles obtained by the treatment method is obviously reduced, and the stability is obviously improved.
Description
Technical Field
The invention relates to the field of feed drug processing, in particular to a preparation method of enramycin premix.
Background
Enramycin, also known as enramycin, duramycin, is obtained by fermenting actinomycetes, and is a polypeptide antibiotic with unsaturated fatty acid combined with more than ten amino acids. Originally developed by the chemical industry Co., ltd. In Japanese Wuta-tsu in 1966. Enramycin has strong inhibition effect on gram-positive bacteria, is not easy to generate drug resistance after long-term use, can change bacterial community distribution in intestinal tracts, is favorable for digestion and absorption of feed nutrient components, promotes animal weight gain and improves feed utilization rate, and has no residue in livestock bodies. The application effect in livestock and poultry production is obvious. Thus, it is used as an antibiotic growth promoter by many countries around the world.
Although enramycin has good development prospect at home and abroad, because enramycin is added with a large amount of culture medium in the fermentation process, the culture medium contains a large amount of solids, so that the enramycin has low yield and low content, the instability of the product is caused, the content is degraded after the enramycin is placed for a period of time, the stability of the enramycin in the storage and transportation processes is affected, and the quality, sales and use of the enramycin are further affected.
Disclosure of Invention
The invention solves the problems by a preparation method of enramycin premix, and in order to achieve the purposes, the invention provides the following technical scheme:
the preparation method of the enramycin premix is characterized by comprising the following steps: (1) carrying out plate-frame filtration on enramycin fermentation liquor; (2) Flash drying the obtained filter cake to obtain the mycelium of enramycin: (3) Diluting mycelium and auxiliary materials in proportion, and fully stirring: (4) Uniformly stirring the enramycin diluent, and then drying to obtain enramycin premix;
the weight ratio of the enramycin mycelium to the auxiliary materials in the step (3) is 4:6;
the auxiliary materials added in the step (3) are glycerides.
The optimization of the invention is that according to the preparation method of the enramycin premix, the pressure of the plate and frame filtration in the step (1) is 0.4-0.6MPa, the filter pressing time is 1-3 hours, and the blowing time is 0.5-1.0 hours.
The optimization of the invention is characterized in that the flash evaporation drying air inlet temperature in the step (2) is 90-150 ℃ and the air outlet temperature is 60-90 ℃ according to the preparation method of the enramycin premix.
The optimization of the invention is characterized in that the preparation method of the enramycin premix is characterized in that the auxiliary materials are added in the step (3) and then stirred to be fully and uniformly stirred for 0.5-2 hours; the optimization of the invention is characterized in that the dryer in the step (4) is a spray dryer according to the preparation method of the enramycin premix.
Aiming at the problem of poor stability of finished enramycin premix products, the invention provides an enramycin treatment method which is simple and feasible, low in cost and pollution-free, and the degradation degree of finished enramycin premix particles obtained by the treatment method is obviously weakened, and the stability is obviously improved.
Detailed Description
The technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The enramycin fermentation broth used in the embodiment of the invention is a fermentation broth prepared by fermenting Streptomyces fungicidal (Streptomyces fungicidicus) serving as a strain.
Taking 3 batches of fermented fermentation liquor, dividing each batch of fermentation liquor into two parts, and simultaneously carrying out a control experiment and preparing the enramycin premix according to the invention by the following steps:
embodiment one:
control group: the traditional process comprises the following steps: the test steps are (1) carrying out plate-frame filtration on enramycin fermentation liquor, wherein the pressure of the plate-frame filtration is 0.4MPa, the filter pressing time is 3h, and the air blowing time is 1.0h; (2) And flash drying the obtained filter cake to obtain the mycelium of enramycin, wherein the air inlet temperature is 90-150 ℃ and the material temperature is 60-90 ℃.
Experimental group: the invention is used: the preparation method of the enramycin premix is characterized by comprising the following steps: (1) carrying out plate-frame filtration on enramycin fermentation liquor; (2) Flash drying the obtained filter cake to obtain the mycelium of enramycin: (3) Diluting mycelium and auxiliary materials in proportion, and fully stirring: (4) Uniformly stirring the enramycin diluent, and then drying to obtain enramycin premix;
the weight ratio of the enramycin mycelium to the auxiliary materials in the step (3) is 4:6;
the auxiliary material added in the step (3) is triglyceride.
Wherein the pressure of the plate frame filtration is 0.4MPa, the filter pressing time is 3h, and the blowing time is 1.0h; the flash drying air inlet temperature is 90-150 ℃, and the air outlet temperature is 60-90 ℃; stirring after adding auxiliary materials to make the auxiliary materials fully and uniformly stirred for 1 hour; the dryer in the step (4) is a spray dryer.
Embodiment two:
control group: the traditional process comprises the following steps: the test steps are (1) carrying out plate-frame filtration on enramycin fermentation liquor, wherein the pressure of the plate-frame filtration is 0.6MPa, the filter pressing time is 1h, and the air blowing time is 0.5h; (2) And flash drying the obtained filter cake to obtain the mycelium of enramycin, wherein the air inlet temperature is 90-150 ℃ and the material temperature is 60-90 ℃. Experimental group: the invention is used: the preparation method of the enramycin premix is characterized by comprising the following steps: (1) carrying out plate-frame filtration on enramycin fermentation liquor; (2) Flash drying the obtained filter cake to obtain the mycelium of enramycin: (3) Diluting mycelium and auxiliary materials in proportion, and fully stirring: (4) Uniformly stirring the enramycin diluent, and then drying to obtain enramycin premix;
the weight ratio of the enramycin mycelium to the auxiliary materials in the step (3) is 4:6;
the auxiliary material added in the step (3) is phosphoglyceride.
Wherein the pressure of the plate frame filtration is 0.6MPa, the filter pressing time is 1h, and the blowing time is 0.5h; the flash drying air inlet temperature is 90-150 ℃, and the air outlet temperature is 60-90 ℃; stirring after adding auxiliary materials to make the auxiliary materials fully and uniformly stirred for 1 hour; the dryer in the step (4) is a spray dryer.
Embodiment III:
control group: the traditional process comprises the following steps: the test steps are (1) carrying out plate-frame filtration on enramycin fermentation liquor, wherein the pressure of the plate-frame filtration is 0.5MPa, the filter pressing time is 1.5h, and the air blowing time is 1h; (2) And flash drying the obtained filter cake to obtain the mycelium of enramycin, wherein the air inlet temperature is 90-150 ℃ and the material temperature is 60-90 ℃.
Test group: the invention is used: the preparation method of the enramycin premix is characterized by comprising the following steps: (1) carrying out plate-frame filtration on enramycin fermentation liquor; (2) Flash drying the obtained filter cake to obtain the mycelium of enramycin: (3) Diluting mycelium and auxiliary materials in proportion, and fully stirring: (4) Uniformly stirring the enramycin diluent, and then drying to obtain enramycin premix;
the weight ratio of the enramycin mycelium to the auxiliary materials in the step (3) is 4:6;
the auxiliary material added in the step (3) is trioctyl monocaprylate.
Wherein the pressure of the plate frame filtration is 0.5MPa, the filter pressing time is 1.5h, and the blowing time is 1h; the flash drying air inlet temperature is 90-150 ℃, and the air outlet temperature is 60-90 ℃; stirring after adding auxiliary materials to make the auxiliary materials fully and uniformly stirred for 1 hour; the dryer in the step (4) is a spray dryer. After the enramycin premix prepared according to the steps is stored in a stability test box for a period of time, the content and the moisture are detected respectively, the stability is compared, and the obtained control data are shown in the attached tables 1 and 2:
as can be seen from the comparison of the data in tables 1 and 2, the content degradation amplitude of the enramycin premix prepared by the invention is small and the stability is greatly improved compared with the enramycin premix prepared by the conventional process in the control group after the storage time is 3 months.
TABLE 1 Enramycin premix prepared by conventional process
TABLE 2 Enramycin premix prepared according to the invention
The present invention is not limited to the details of the above-described exemplary embodiments, and can be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Claims (3)
1. The preparation method of the enramycin premix is characterized by comprising the following steps: (1) carrying out plate-frame filtration on enramycin fermentation liquor; (2) Flash drying the obtained filter cake to obtain the mycelium of enramycin: (3) Diluting mycelium and auxiliary materials in proportion, and fully stirring: (4) Uniformly stirring the enramycin diluent, and then drying by using a spray dryer to obtain enramycin premix;
the weight ratio of the enramycin mycelium to the auxiliary materials in the step (3) is 4:6;
the auxiliary materials added in the step (3) are triglyceride;
the stirring time in the step (3) is 0.5-2 hours.
2. The method for preparing enramycin premix according to claim 1, wherein the pressure of the plate-and-frame filtration in the step (1) is 0.4-0.6MPa, the press filtration time is 1-3 hours, and the blowing time is 0.5-1.0 hours.
3. The method for preparing enramycin premix according to claim 1, wherein the flash drying inlet air temperature in the step (2) is 90-150 ℃ and the outlet air temperature is 60-90 ℃.
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| CN201610505031.1A CN105999220B (en) | 2016-06-30 | 2016-06-30 | Preparation method of enramycin premix |
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| CN105999220B true CN105999220B (en) | 2023-06-09 |
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| CN108059657A (en) * | 2018-01-18 | 2018-05-22 | 山西新源华康化工股份有限公司 | Production process for preventing enramycin product from degrading |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101234097A (en) * | 2007-01-30 | 2008-08-06 | 佛山市海纳川药业有限公司 | Kitasamycin microcapsule preparation and preparation and application thereof |
| CN101862443A (en) * | 2010-06-11 | 2010-10-20 | 濮阳泓天威药业有限公司 | Preparation method of enramycin premix |
| CN105104761A (en) * | 2015-09-09 | 2015-12-02 | 石家庄高科动物保健品有限公司 | Method for preparing enramycin premix |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| JPS57165322A (en) * | 1981-04-02 | 1982-10-12 | Nippon Kayaku Co Ltd | Oral medication composition for domestic animal and domestic fowl |
| CN103961316B (en) * | 2014-05-13 | 2020-01-07 | 浙江工商大学 | Microemulsion containing enramycin and preparation method thereof |
| CN104491837B (en) * | 2014-10-30 | 2017-02-15 | 广东海纳川生物科技股份有限公司 | Antibacterial peptide preparation, and preparation method and application thereof |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101234097A (en) * | 2007-01-30 | 2008-08-06 | 佛山市海纳川药业有限公司 | Kitasamycin microcapsule preparation and preparation and application thereof |
| CN101862443A (en) * | 2010-06-11 | 2010-10-20 | 濮阳泓天威药业有限公司 | Preparation method of enramycin premix |
| CN105104761A (en) * | 2015-09-09 | 2015-12-02 | 石家庄高科动物保健品有限公司 | Method for preparing enramycin premix |
Non-Patent Citations (1)
| Title |
|---|
| 刘雅敏等.单硬脂酸甘油酯.《药用制剂常用辅料》.天津科技翻译出版公司,1994,(第1版),第151-152页. * |
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