CN105994389A

CN105994389A - Green walnut peel extract and application method thereof in tobacco aphid damage

Info

Publication number: CN105994389A
Application number: CN201610448438.5A
Authority: CN
Inventors: 陈明山; 何念武; 王攀; 张永锋; 黄金辉; 穆耀辉; 风高峰
Original assignee: SHANGLUO COMPANY SHAANXI COUNTY TOBACCO Co Ltd; Shangluo University
Current assignee: SHANGLUO COMPANY SHAANXI COUNTY TOBACCO Co Ltd; Shangluo University
Priority date: 2016-06-21
Filing date: 2016-06-21
Publication date: 2016-10-12
Anticipated expiration: 2036-06-21
Also published as: CN105994389B

Abstract

The invention provides a green walnut peel extract and an application method thereof in tobacco aphid damage. Outer green walnut peel is dried and smashed and then is extracted with petroleum ether and ethyl acetate, and the green walnut peel extract is obtained; after being dissolved and diluted, the green walnut peel extract can be used for preventing and treating tobacco aphid damage. The extraction method of the green walnut peel extract is simple and easy to implement, the green walnut peel is turned into wealth, and the utilization rate of the green walnut peel is increased. Besides, the green walnut peel extract is high in tobacco aphid killing rate, good in prevention and treatment effect and long in pesticide effect lasting time.

Description

A kind of walnut peel extract and the application process in tobacco aphid disease thereof

Technical field

The invention belongs to plant extract field, be specifically related to a kind of walnut peel extract and in tobacco aphid disease In application process.

Background technology

Exocarpium Juglandis Immaturus, has another name called Exocarpium Juglandis Immaturum, for juglandaceae plant Juglans mandshurica (Juglans Mandshurica M.) and Semen Juglandis (Juglans RegialThe green outer peel of immature fruit L.), main product in northeast, Shandong, river The ground such as north, the most always as parts of generic medicinal plants among the people, pungent, bitter, puckery, flat, there is heat-clearing and toxic substances removing, only dispel the wind treatment tinea, pain relieving The medical value widely such as dysentery.Research shows, Exocarpium Juglandis Immaturum effective ingredient is protected and antimicrobial acivity for the healthy of the mankind Aspect has important effect.Exocarpium Juglandis Immaturus is the green peel that the outside thick layer of Semen Juglandis is thick, and Walnut Fruit is taken off after plucking Pericarpium Citri Reticulatae Viride is deposited in field or is poured in river, not only results in the waste of resource, also can bring to ecological environment serious Pollute.If to the active substance in Exocarpium Juglandis Immaturus in addition extracting and developing, comprehensive utilization, environment both can have been protected, it is also possible to increase Add orchard worker's income, wouldn't be to kill two birds with one stone.

Cigarette aphid occurs the most generally and day by day serious in Ge Chanyan district, the whole nation, and annual 6～cause harm on Nicotiana tabacum L. July and contain most, 7 Produce alatae from vega after the last ten-days period moon and shift vegetable of causing harm, enter to late October and survive the winter.It is each that cigarette aphid has a strong impact on China The yield of tobacco in Chan Yan district and quality.

Pesticide plays an important role in integrated pest prevention and control, but commonly using along with chemical pesticide, 3R (Resistance, Resurgence, Residue) phenomenon, i.e. resistance, second rice crop season and residual phenomena are also on the rise, and have Serious side effect.Find efficiently from plant, low toxicity, low-residual and the good new natural active products of being compatible with the environment property Matter, is applied to pesticide significant in the prevention and control of plant diseases, pest control.And it is sick at preventing and treating tobacco aphid about walnut peel extract Application, relevant report does not occur.

Summary of the invention

It is an object of the invention to provide a kind of walnut peel extract, and this walnut peel extract is at tobacco aphid Application process in disease.

The present invention is achieved through the following technical solutions.

A kind of walnut peel extract, through the following steps that preparation:

(1) taking Semen Juglandis outer layer Pericarpium Citri Reticulatae Viride, ventilation is dried, never color blackening, then dries at 75-90 DEG C, pulverizes, mistake after cooling 60 ~ 100 mesh sieves, obtain being in store under Exocarpium Juglandis Immaturus powder, room temperature；

(2) weighing a certain amount of Exocarpium Juglandis Immaturus powder in container, 1:2 adds petroleum ether, reflux, extract, at 45 DEG C by volume 2h, carries out defat, filters, reclaim the petroleum ether in filtrate after being cooled to room temperature, and filtering residue is standby；

(3) filtering residue in step (2) is taken, then according to volume ratio 1:5-10 adds ethyl acetate solution, 70-80 DEG C of reflux, extract, 5h, filters, and filtrate and filtering residue are standby；

(4) ratio taking Exocarpium Juglandis Immaturus filtering residue 1:3-6 by volume in step (3) adds ethyl acetate, extracts, at 70-80 DEG C Reflux, extract, 2h, filters, and repeats this operation 3-5 time；

(5) filtrate in above-mentioned steps (3) and (4) is merged, concentrate, remove solvent, obtain walnut peel extract.

Preferably, the boiling spread of preparation process (2) the described petroleum ether of above-mentioned walnut peel extract is: 30-60 DEG C.

Preferably, the ethyl acetate concentration that the preparation process (3) of above-mentioned walnut peel extract adds is 50%-70%.

Preferably, the ethyl acetate concentration that the preparation process (4) of above-mentioned walnut peel extract adds is 80%-90%.

A kind of above-mentioned walnut peel extract application process in tobacco aphid disease is as follows:

(1) being dissolved by walnut peel extract, obtain Exocarpium Juglandis Immaturus solution, concentration is 10-30mg/mL；

(2) Exocarpium Juglandis Immaturus solution is diluted 250-300 times, in the Sheng phase first generation 1 day old larva beginning, select fair weather to be sprayed on Tobacco leaf surface and the back side.

Preferably, in above-mentioned walnut peel extract application process in tobacco aphid disease, step (1) uses 5% Walnut peel extract is dissolved by dimethyl sulfoxide (DMSO).

The invention have the advantages that

Preventing and treating tobacco aphid disease is had a significant effect, with traditional imidacloprid by the walnut peel extract that the present invention provides Comparing, the walnut peel extract of the present invention reaches more than 99% to the control rate of cigarette aphid, and duration of efficacy is long, 25 days Time still there is good effect.It is raw material that the present invention reclaims discarded Exocarpium Juglandis Immaturus, and extracting method is simple to operate, by Exocarpium Juglandis Immaturus Turn waste into wealth, improve its utilization rate, protect ecological environment.

Detailed description of the invention

Below in conjunction with specific embodiment, the present invention is described in detail.

Embodiment 1

(1) taking Semen Juglandis outer layer Pericarpium Citri Reticulatae Viride, ventilation is dried, never color blackening, then dries at 75 DEG C, pulverizes after cooling, crosses 60 Mesh sieve, obtains being in store under Exocarpium Juglandis Immaturus powder, room temperature；

(2) weighing a certain amount of Exocarpium Juglandis Immaturus powder in container, 1:2 adds petroleum ether, the boiling spread of petroleum ether by volume For: 30-60 DEG C, reflux, extract, 2h at 45 DEG C, carry out defat, filter after being cooled to room temperature, reclaim the petroleum ether in filtrate, filter Slag is standby；

(3) take filtering residue in step (2), add the ethyl acetate solution of 50% according to volume ratio 1:5, in 70 DEG C of reflux, extract, 5h, mistake Filter, filtrate and filtering residue are standby；

(4) ratio taking Exocarpium Juglandis Immaturus filtering residue 1:3 by volume in step (3) adds the ethyl acetate of 80%, extracts, at 70 DEG C Reflux, extract, 2h, filters, and repeats this operation 3 times；

Embodiment 2

(1) taking Semen Juglandis outer layer Pericarpium Citri Reticulatae Viride, ventilation is dried, never color blackening, then dries at 90 DEG C, pulverizes after cooling, crosses 100 Mesh sieve, obtains being in store under Exocarpium Juglandis Immaturus powder, room temperature；

(3) take filtering residue in step (2), add the ethyl acetate solution of 70% according to volume ratio 1:10,80 DEG C of reflux, extract, 5h, Filtering, filtrate and filtering residue are standby；

(4) ratio taking Exocarpium Juglandis Immaturus filtering residue 1:6 by volume in step (3) adds the ethyl acetate of 90%, extracts, at 80 DEG C Reflux, extract, 2h, filters, and repeats this operation 3 times；

Embodiment 3

(1) taking Semen Juglandis outer layer Pericarpium Citri Reticulatae Viride, ventilation is dried, never color blackening, then dries at 85 DEG C, pulverizes after cooling, crosses 80 Mesh sieve, obtains being in store under Exocarpium Juglandis Immaturus powder, room temperature；

(3) take filtering residue in step (2), add the ethyl acetate solution of 60% according to volume ratio 1:5, in 75 DEG C of reflux, extract, 5h, mistake Filter, filtrate and filtering residue are standby；

(4) ratio taking Exocarpium Juglandis Immaturus filtering residue 1:4 by volume in step (3) adds the ethyl acetate of 85%, extracts, at 75 DEG C Reflux, extract, 2h, filters, and repeats this operation 3 times；

Above-mentioned walnut peel extract 5%DMSO solution is configured to the solution of 20mg/mL, fully dissolves, each dilute 250 times.

Embodiment 4

The walnut peel extract of the present invention application process in tobacco aphid disease and prevention effect

1. test material

(1) for examination cigarette strain: 60 d tobacco seedlings

(2) controlling object: tobacco aphid first brood of larvae

(3) reagent agent: imidacloprid, the walnut peel extract that embodiment 1,2,3 prepares.

2. EXPERIMENTAL DESIGN

This test sets 4 kinds, 2 class chemicals treatment altogether: concentration is all as 20mg/mL, and a class is that commercially available tradition pesticide imidacloprid is as right According to group, another kind of is that the Exocarpium Juglandis Immaturus that 3 kinds of walnut peel extracts that embodiment 1,2,3 prepares prepare with the DMSO dissolving of 5% carries Take liquid.Separately setting the DMSO of 5% as blank group, every kind of process is repeated 5 times, and each is repeated as 1 group, and often group is that 20 strain cigarettes are wonderful, respectively Medicament is identical with the extension rate of blank group, all dilutes 250 times.

3. application method

In the Sheng phase first generation 1 day old larva beginning, select fair weather that each group medicament diluted is sprayed on the Nicotiana tabacum L. of aphid Tow sides.

4. drug effect inspection

Select the blade face that often group aphid is more as fixed sample, use label labelling, indicate numbering, nomenclature of drug.Adjust before dispenser Look into the Aphed population on blade face.1d, 2d, 4d, 8d, 16d, 25d, 32d after spray medicine checks respectively for often organizing children fixed sample Worm borer population mesh alive.

5. result

Table 1 different agents prevention effect to cigarette aphid

From table 1 it follows that two class medicaments have obvious prevention effect to tobacco aphid, compared with tradition pesticide, this The walnut peel extract of invention preparation is more preferable to the prevention effect of tobacco aphid, and not only killing rate is high, cigarette aphid prevents effect The best, instant effect, and duration of efficacy is long, and when spraying medicine 25d, prevention effect remains to reach about 99%, as spray medicine 32d Rear prevention effect has declined, and is drug effect performance reason totally, and this explanation is necessary that carrying out secondary spray medicine imitates to strengthen preventing and treating Really.And imidacloprid just has the performance that drug effect declines after spray medicine 16d.

Claims

1. a walnut peel extract, it is characterised in that: this walnut peel extract is made by the steps:

(2) weighing in a certain amount of Exocarpium Juglandis Immaturus powder, 1:2 adds petroleum ether, reflux, extract, 2h at 45 DEG C, cooling by volume Filtering to room temperature, reclaim the petroleum ether in filtrate, filtering residue constant weight is standby；

(4) taking filtering residue in step (3), the ratio of 1:3-6 adds ethyl acetate by volume, in 70-80 DEG C of reflux, extract, 2h, mistake Filter, repeats this operation 3-5 time；

2. the walnut peel extract described in a claim 1, it is characterised in that: the preparation process that this Exocarpium Juglandis Immaturus extracts In, the boiling spread of step (2) described petroleum ether is: 30-60 DEG C.

3. the walnut peel extract described in a claim 1 or 2, it is characterised in that: the preparation step that this Exocarpium Juglandis Immaturus extracts In Zhou, the ethyl acetate concentration that step (3) adds is 50%-70%.

4. the walnut peel extract described in a claim 3, it is characterised in that: the preparation process that this Exocarpium Juglandis Immaturus extracts In, the ethyl acetate concentration that step (4) adds is 80%-90%.

5. the application process in tobacco aphid disease of the walnut peel extract described in a claim 4, it is characterised in that Concrete grammar is as follows:

6. the application process in tobacco aphid disease of the walnut peel extract described in a claim 5, it is characterised in that Walnut peel extract is dissolved by the dimethyl sulfoxide (DMSO) using 5% in step (1).