CN105993933A - Cultivation method for mulberry chromosome doubling - Google Patents
Cultivation method for mulberry chromosome doubling Download PDFInfo
- Publication number
- CN105993933A CN105993933A CN201610445234.6A CN201610445234A CN105993933A CN 105993933 A CN105993933 A CN 105993933A CN 201610445234 A CN201610445234 A CN 201610445234A CN 105993933 A CN105993933 A CN 105993933A
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- China
- Prior art keywords
- grafting
- mulberry
- chromosome doubling
- cultivation method
- induction rate
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
- A01H1/08—Methods for producing changes in chromosome number
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/30—Grafting
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
A cultivation method for mulberry chromosome doubling includes the following steps that firstly, stem tips of grafting seedlings of a target parent in the current year are subjected to continuous five-day instilling treatment by means of colchicines with the concentration gradient from 0.4% to 0.1%, wherein the grafting seedlings are 20-30 cm high; secondly, during treatment, kraft bags or envelopes are adopted for moisture retention and shading; thirdly, shoots germinating below treated portions are cleaned in time after treatment; fourthly, in the next year, abnormal leaves and branches are subjected to single-bud separation and grafting. The method has the advantage of being high in polyploidy induction rate. Experiments prove that the polyploidy induction rate can reach 32-56% by grafting individuals through the cultivation method for mulberry chromosome doubling, and the average polyploidy induction rate reaches 47% and is much higher than the domestic and international level.
Description
Technical field
The present invention relates to the breeding method of a kind of mulberry artificial polyploid, be specifically related to a kind of mulberry
The breeding method of chromosome doubling.
Background technology
Mulberry polyploid has the advantages such as growth vigorous, the food value of leaf is excellent, resistance enhancing, is Mulberry
The Main way of tree breeding.At present, the common method cultivating mulberry artificial polyploid is to use the autumn
Tazettine processes mulberry seed as mutagenic agent or grows bud, but for a long time, polyploid mutagenesis rate
(obtaining polyploid success rate) is always below 10%.Studying carefully its one of the main reasons is Colchicum autumnale
Alkali has physiological-toxicity, deals with improperly and causes process individual death；Two be the pharmacy effect time
Not；Three be that variation organ does not in time separate from parent.
Summary of the invention
The technical problem to be solved is to provide the mulberry that a kind of multiploid induction rate is high
The breeding method of chromosome doubling.
The present invention solves its technical problem and employed technical scheme comprise that, a kind of mulberry chromosome doubling
Breeding method, comprise the following steps:
(1) by use Concentraton gradient from high to low 0.4～0.1% colchicine to target parent work as
The stem apex of year grafting carries out the instillation of continuous 4～6 days (preferential 5 days) and processes, and can avoid
Physiological-toxicity is excessive causes death, wherein, and grafting height of seedling 20～30cm；
(2) strengthen moisturizing during processing and shelter from heat or light, being beneficial to extend the pharmacy effect time；
(3) last cleaning in time processes the anvil bud that below position is sprouted, and abnormal leaf branch section is carried out by next year
Simple bud separates grafting, and variation organ can be made to separate in time.
The present invention has the advantages that multiploid induction rate is high.It is demonstrated experimentally that use the Mulberry of the present invention
The breeding method grafting individuality of tree chromosome doubling obtains chromosome doubling rate (i.e. multiploid induction
Rate) can reach 32～56%, averagely reach 47%, the most domestic and international level.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is illustrated further.
Embodiment 1
(1) Concentraton gradient 0.4%, 0.3%, 0.2% and 0.1% colchicine from high to low will be used
The instillation carried out the stem apex of target parent grafting then continuous 5 days processes, wherein, and grafting
Height of seedling 20～30cm；
(2) kraft paper bag or envelope is used to carry out moisturizing and shelter from heat or light during processing；
(3) after processing, cleaning in time processes the anvil bud that below position is sprouted；
(4) abnormal leaf branch section is carried out simple bud separation grafting by next year.
Tests prove that, the present embodiment grafting individuality obtain chromosome doubling rate can reach 32～
56%, averagely reach 47%.
Claims (2)
1. the breeding method of a mulberry chromosome doubling, it is characterised in that comprise the following steps:
(1) by use Concentraton gradient from high to low 0.4～0.1% colchicine to target parent work as
The instillation that the stem apex of year grafting is carried out continuous 4～6 days processes, wherein, grafting height of seedling 20～
30cm:
(2) kraft paper bag or envelope is used to carry out moisturizing and shelter from heat or light during processing；
(3) after processing, cleaning in time processes the anvil bud that below position is sprouted；
(4) abnormal leaf branch section is carried out simple bud separation grafting by next year.
The breeding method of mulberry chromosome doubling the most according to claim 1, it is characterised in that
In step (1), the described continuous drip process time is 5 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610445234.6A CN105993933A (en) | 2016-06-20 | 2016-06-20 | Cultivation method for mulberry chromosome doubling |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610445234.6A CN105993933A (en) | 2016-06-20 | 2016-06-20 | Cultivation method for mulberry chromosome doubling |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105993933A true CN105993933A (en) | 2016-10-12 |
Family
ID=57085585
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610445234.6A Pending CN105993933A (en) | 2016-06-20 | 2016-06-20 | Cultivation method for mulberry chromosome doubling |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105993933A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106508390A (en) * | 2016-10-28 | 2017-03-22 | 邓凯 | Rootstock cultivating method and plant grafting method |
| CN110199880A (en) * | 2019-07-09 | 2019-09-06 | 内蒙古和盛生态育林有限公司 | A kind of cultural method covering mulberry polyploid seedling |
| CN111328712A (en) * | 2020-04-08 | 2020-06-26 | 浙江省农业科学院 | Supplementary device that presents of mulberry winter bud medicament treatment |
| CN111387054A (en) * | 2020-04-20 | 2020-07-10 | 广西壮族自治区蚕业技术推广站 | Method for performing ploidy induction on mulberry seedling chromosome by using cotton sleeving tube |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101699988A (en) * | 2009-12-01 | 2010-05-05 | 四川省丝绸科学研究院 | Selecting and breeding method of polyploidy novel mulberry tree breed |
| CN105123511A (en) * | 2015-09-08 | 2015-12-09 | 安徽省农业科学院蚕桑研究所 | Method for breeding mulberry polyploids through chemical mutagenesis |
-
2016
- 2016-06-20 CN CN201610445234.6A patent/CN105993933A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101699988A (en) * | 2009-12-01 | 2010-05-05 | 四川省丝绸科学研究院 | Selecting and breeding method of polyploidy novel mulberry tree breed |
| CN105123511A (en) * | 2015-09-08 | 2015-12-09 | 安徽省农业科学院蚕桑研究所 | Method for breeding mulberry polyploids through chemical mutagenesis |
Non-Patent Citations (3)
| Title |
|---|
| 孙日彦等: "化学诱变获得桑树四倍体研究", 《烟台师范学院学报（自然科学版）》 * |
| 焦锋等: "桑树多倍体鉴定和育种研究现状", 《西北农业学报》 * |
| 苏超等: "人工无性系四倍体桑品种陕桑402的选育", 《蚕业科学》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106508390A (en) * | 2016-10-28 | 2017-03-22 | 邓凯 | Rootstock cultivating method and plant grafting method |
| CN110199880A (en) * | 2019-07-09 | 2019-09-06 | 内蒙古和盛生态育林有限公司 | A kind of cultural method covering mulberry polyploid seedling |
| CN110199880B (en) * | 2019-07-09 | 2021-03-23 | 内蒙古和盛生态育林有限公司 | Method for culturing mulberryne polyploid seedlings |
| CN111328712A (en) * | 2020-04-08 | 2020-06-26 | 浙江省农业科学院 | Supplementary device that presents of mulberry winter bud medicament treatment |
| CN111328712B (en) * | 2020-04-08 | 2021-08-03 | 浙江省农业科学院 | Supplementary device that presents of mulberry winter bud medicament treatment |
| CN111387054A (en) * | 2020-04-20 | 2020-07-10 | 广西壮族自治区蚕业技术推广站 | Method for performing ploidy induction on mulberry seedling chromosome by using cotton sleeving tube |
| CN111387054B (en) * | 2020-04-20 | 2022-02-01 | 广西壮族自治区蚕业技术推广站 | Method for performing ploidy induction on mulberry seedling chromosome by using cotton sleeving tube |
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Application publication date: 20161012 |