CN105974065A - Method for measuring biological density of bulking sludge through high-density microsphere method - Google Patents
Method for measuring biological density of bulking sludge through high-density microsphere method Download PDFInfo
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- CN105974065A CN105974065A CN201610423199.8A CN201610423199A CN105974065A CN 105974065 A CN105974065 A CN 105974065A CN 201610423199 A CN201610423199 A CN 201610423199A CN 105974065 A CN105974065 A CN 105974065A
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Abstract
The invention provides a method for measuring the biological density of bulking sludge through a high-density microsphere method and relates to the field of sludge biological treatment. After the bulking sludge with a loose structure is subjected to solid-liquid separation, the bulking sludge is washed and diluted to the original volume, and the sludge is equally divided into at least 6 parts at average; high-density ceramic microspheres with different mass gradients are added into the equally-divided sludge samples respectively, and normal-temperature stirring is conducted, so that the sludge samples and the microspheres are mixed to be uniform and are tightly combined; DMS standard solutions with different densities are prepared; 1 mL of the microsphere sludge sample mixed solution sample is taken and put in at least 3 mL of three DMS standard solutions with the similar densities, and standing is carried out after shaking up is finished; the positions of the samples are observed, and the biological densities p1 to pn of the samples are obtained; the biological densities of the samples and the mass of the added microspheres are subjected to data reduction and analysis, and the original biological density of the loose sludge without the microspheres is obtained through calculation. According to the method, the bulking sludge with the loose structure can be subjected to biological density measurement, the floc structure of the bulking sludge can be more compact, and sludge layering during measurement is avoided.
Description
Technical field
The present invention relates to field of biological sewage treatment, it is provided that it is raw that a kind of high density microsphere method measures bulking sludge
The method of thing density, can carry out biological density mensuration to loosely organized bulking sludge, and the method can make
Bulking sludge flocculation structure is tightr, can prevent bulking sludge due to loosely organized and during causing measuring
Mud lamination problem, measure for loosely organized bulking sludge biological density and a kind of prioritization scheme be provided.
Background technology
Sludge bulking problem uses Activated Sludge Process to process each size sewage of sewage the most both at home and abroad
Treatment plant's FAQs, the feature of sludge bulking includes that the universality occurred is higher, and harm is serious, in a large number
Sludge loss can cause the drastically decline of system treatment effect, can cause the collapse of system time serious, one
Denier is difficult to control to and to recover required time the longest.Therefore sludge bulking problem is furtherd investigate
And then exploitation is significant to its prevention and controls.
At present, the conventional index evaluating sludge settling property is sludge volume index (Sludge Volume
Index, SVI), refer to the mud mixed liquid of Aeration tank outlet through 30min quiet heavy after, every gram of dewatered sludge
Shared volume, it is generally recognized that there occurs sludge bulking when the SVI of activated sludge is higher than 150mL/g.
Currently lacking the analysis to other character of bulking sludge and research, wherein biological density can characterize well
The behaviors of settlement of mud flco, contributes to studying floating and the wastage of bulking sludge.
Structure yet with some bulking sludge is the loosest, there will be mud during conventional determining
The phenomenon of layering, causes it being carried out the mensuration of biological density.Therefore, for prevent bulking sludge by
In loosely organized and cause the mud layering during measuring to develop for loosely organized bulking sludge raw
The assay method of thing density is the most necessary.
Summary of the invention
For above-mentioned phenomenon weak point, it is raw that the present invention provides a kind of high density microsphere method to measure bulking sludge
The method of thing density, can carry out biological density mensuration to loosely organized bulking sludge, and the method can make
Bulking sludge flocculation structure is tightr, can prevent bulking sludge due to loosely organized and during causing measuring
Mud lamination problem, measure for loosely organized bulking sludge biological density and a kind of prioritization scheme be provided.
A kind of high density microsphere method measures the method for bulking sludge biological density, it is characterised in that: include following
Step:
(1), loosely organized bulking sludge (average SVI value is more than 150mL/g) centrifugal solid-liquid is divided
From rear, wash three times with the former reactor water outlet through filtration treatment, then former when being diluted to non-solid-liquid separation
Volume, is averagely divided at least 6 parts；
(2) in the mud sample that step (1) decile is good, add the high density pottery of different quality gradient respectively
Porcelain microsphere, mixes with microsphere to mud sample by agitator stirring at normal temperature 5-15min under 100-300rpm rotating speed
Uniformly, and microsphere is tightly combined with mud；The density of high density ceramic microsphere refers generally to more than 1.3g/
mL。
(3) DMS (the density measur of a series of different densities, is prepared according to test concrete condition
Ement solutions) mark liquid；
(4), for every part of microsphere mud sample mixed liquor sample of step (2), microsphere mud sample mixed liquor is taken respectively
Sample 1mL is respectively placed in three DMS mark liquid closing on density of at least 3mL, stands 1 after shaking up
0-30min；Observe microsphere mud sample present position, if microsphere mud sample is suspended in certain marks liquid central authorities, then this DM
The density of S mark liquid is the biological density of this microsphere mud sample；If microsphere mud sample is positioned at bottom a mark liquid, drift
Float on adjacent low concentration mark liquid top, then density and microsphere mud sample position according to these two DMS mark liquid are estimated
Read its biological density；Close on bottom density mark liquid if microsphere mud sample sinks to three, then change concentration higher
Three proximity density DMS mark liquid be measured；If microsphere mud sample swims in three closes on density mark liquid
Top, then the DMS mark liquid changing three lower proximity density of density is measured；
(5), operated by step (4), until the biological density reading each microsphere mud sample is respectively ρ1
To ρn；The microsphere mud sample biological density recorded and the quality adding microsphere are carried out data compilation, and maps
Analyze, the linear relationship obtaining the mud sample mixed liquor sample biological density adding microsphere with adding microspheres quality,
Microsphere loose mud primitive organism density is not added by being calculated.
Compared with prior art, the invention have the advantages that
(1) method that the high density microsphere method that the present invention provides measures bulking sludge biological density, can be right
Loosely organized bulking sludge carries out biological density mensuration, and the method can make bulking sludge flocculation structure tighter
Close, can prevent bulking sludge due to loosely organized and cause measure during mud lamination problem.
(2) method of the high density microsphere method mensuration bulking sludge biological density that this invention provides not only is suitable for
Bulking sludge biological density in different degrees of expansion measures, and is simultaneously applicable to dissimilar include bacterium glue
Group and hyphomycetic bulking sludge biological density measure.
Accompanying drawing explanation
Fig. 1 is that the embodiment of the present invention 1 Gram’s staining high density microsphere is combined figure (1000 times) with mud flco
(arrow indication is W210 ceramic microsphere)；
Fig. 2 is mud sample mixed liquor sample biological density and the addition microspheres quality that the embodiment of the present invention 1 adds microsphere
Linear graph.
Detailed description of the invention
With specific implementation method, the present invention is described in further detail below in conjunction with the accompanying drawings, but is not limited to
Following example.
Embodiment 1
1, the basic parameter of bulking sludge is determined: average mixed genetic-neural network (MLSS) is 4000
Mg/L, mean sludge bulk index (SVI) value is 180mL/g；
2, from the reactor that sludge bulking occurs, take this mud sample of 3.5L, after centrifugal solid-liquid separates, use
Wash three times through this reactor water outlet of filtration treatment, then be diluted to 3.5L, be averagely placed on 7 1L
In conical flask, numbering 1-7 conical flask.
3, respectively in 7 conical flasks add 0.04g, 0.08g, 0.12g, 0.16g, 0.2g, 0.24g,
The W210 ceramic microsphere (3M company, density 2.4g/mL) of 0.28g, with magnetic stirring apparatus 200
Under rpm rotating speed, stirring at normal temperature 10min is mixed homogeneously to mud sample with microsphere, microscopic examination microsphere and mud
In conjunction with situation (Fig. 1), find that combination the most closely can carry out lower step test operation.
4, preparation DMS (density measurement solutions) mark liquid, takes Percoll solution (S
Cientan company, density is 1.131g/mL) (close through this reactor water outlet of filtration treatment by step 2
Degree is 1.01g/mL) dilution, the DMS of preparation different densities marks liquid, as shown in table 1.
5, respectively take the DMS mark liquid that 3mL volume fraction is 45%, 50%, 55% and be separately added into three 1
In 5mL centrifuge tube, from No. 1 conical flask, take 1mL sample be respectively sequentially added in three centrifuge tubes, shake
15min is stood after even.
6, observing sample present position, if sample is suspended in certain centrifuge tube central authorities, then this DMS marks liquid
Density is the biological density of this sample.
If 7 samples are positioned at bottom a centrifuge tube, float on adjacent low-density centrifuge tube liquid level top, then
Its biological density of reading is estimated in density and mud sample position according to two centrifuge tube correspondence DMS mark liquid.
If 8 samples sink to bottom three centrifuge tubes, then change the DM of higher three proximity density of density
S mark liquid is measured.
If 9 samples swim in three centrifuge tube liquid level tops, then change three proximity density that density is lower
DMS mark liquid be measured.
10,5-9 step operation is repeated, until the biological density obtaining 1-7 conical flask sample is respectively ρ1、
ρ2、ρ3、ρ4、ρ5、ρ6、ρ7。
11, record No. 1-7 has been added the biological density of microsphere mud sample mixed liquor sample and the matter adding microsphere
Amount carries out data compilation, and mapping analysis, as shown in Figure 2.
12, analyze the biological density finding addition microsphere mud sample mixed liquor sample to become with the quality of addition microsphere
Linear relationship y=0.0273x+1.076, wherein y representative sample biological density, x represents and adds microspheres quality
(R2=0.998) it is, 1.0 by be calculated this reactor not adding microsphere loose mud primitive organism density
76g/mL。
Table 1 is that the DMS of the embodiment of the present invention 1 different volumes mark marks liquid density meter；
All within the spirit and principles in the present invention, any modification, equivalent substitution and improvement etc. made,
Should be included within the scope of the present invention.
Claims (2)
1. the method that high density microsphere method measures bulking sludge biological density, its feature exists
In: comprise the following steps:
(1), by after loosely organized bulking sludge centrifugal solid-liquid separation, with through filtration treatment
Former reactor water outlet wash three times, then original volume when being diluted to non-solid-liquid separation, average etc.
It is divided at least 6 parts；
(2) in the mud sample that step (1) decile is good, add the height of different quality gradient respectively
Density ceramic microsphere, with agitator, under 100-300rpm rotating speed, stirring at normal temperature 5-15min is extremely
Mud sample is mixed homogeneously with microsphere, and microsphere is tightly combined with mud；
(3) DMS (densit of a series of different densities, is prepared according to test concrete condition
Y measurement solutions) mark liquid；
(4), for every part of microsphere mud sample mixed liquor sample of step (2), microsphere mud sample is taken respectively
Mixed liquor sample 1mL is respectively placed in three DMS mark liquid closing on density of at least 3mL,
10-30min is stood after shaking up；Observe microsphere mud sample present position, if microsphere mud sample is suspended in
Certain mark liquid is central, then the density of this DMS mark liquid is the biological density of this microsphere mud sample；If
Microsphere mud sample is positioned at bottom a mark liquid, floats on adjacent low concentration mark liquid top, then according to this
Its biological density of reading is estimated in density and the microsphere mud sample position of two DMS mark liquid；
Close on bottom density mark liquid if microsphere mud sample sinks to three, then change higher three of concentration
The DMS mark liquid of proximity density is measured；If microsphere mud sample swims in three closes on density mark
Liquid top, then the DMS mark liquid changing three lower proximity density of density is measured；
(5), operated by step (4), until the biological density reading each microsphere mud sample divides
Wei ρ1To ρn；By the microsphere mud sample biological density recorded and the quality number adding microsphere
According to arrangement, and mapping analysis, obtain the mud sample mixed liquor sample biological density adding microsphere and add
Entering the linear relationship of microspheres quality, by being calculated, not add microsphere loose mud primitive organism close
Degree.
2. measure bulking sludge according to a kind of high density microsphere method described in claim 1 biological
The method of density, it is characterised in that: the density of high density ceramic microsphere refers generally to more than 1.3g/
mL。
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106554070A (en) * | 2017-01-09 | 2017-04-05 | 苏州科技大学 | Granule sludge etc. point microbial process |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1596224A (en) * | 2002-02-22 | 2005-03-16 | Sut(西拉雅)私人有限公司 | Aerobic biomass granules for waste water treatment |
-
2016
- 2016-06-15 CN CN201610423199.8A patent/CN105974065A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1596224A (en) * | 2002-02-22 | 2005-03-16 | Sut(西拉雅)私人有限公司 | Aerobic biomass granules for waste water treatment |
Non-Patent Citations (4)
| Title |
|---|
| ANDREW J. SCHULER， ET AL.: "Density effects on activated sludge zone settling velocities", 《WATER RESEARCH》 * |
| ANDREW J. SCHULER，ET AL.: "Causes of Variable Biomass Density and Its Effects on Settleability in Full-Scale Biological Wastewater Treatment Systems", 《ENVIRON. SCI. TECHNOL.》 * |
| ANDREW J. SCHULER，ET AL.: "Microsphere addition for the study of biomass properties and density effects on settleability in biological wastewater treatment systems", 《WATER RESEARCH》 * |
| PATRICIA A. JONES，ET AL.: "Seasonal variability of biomass density and activated sludge settleability in full-scale wastewater treatment systems", 《CHEMICAL ENGINEERING JOURNAL》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106554070A (en) * | 2017-01-09 | 2017-04-05 | 苏州科技大学 | Granule sludge etc. point microbial process |
| CN106554070B (en) * | 2017-01-09 | 2019-05-03 | 苏州科技大学 | Granule sludge etc. divides microbial process |
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