CN105968224A - Extraction method of Chinese wolfberry polysaccharides - Google Patents
Extraction method of Chinese wolfberry polysaccharides Download PDFInfo
- Publication number
- CN105968224A CN105968224A CN201610570660.2A CN201610570660A CN105968224A CN 105968224 A CN105968224 A CN 105968224A CN 201610570660 A CN201610570660 A CN 201610570660A CN 105968224 A CN105968224 A CN 105968224A
- Authority
- CN
- China
- Prior art keywords
- supernatant
- raw material
- fructus lycii
- lycium barbarum
- carrying
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses an extraction method of Chinese wolfberry polysaccharides, relating to the technical field of traditional Chinese medicines. The method comprises the following steps: 1. removing impurities in the Chinese wolfberry raw material; 2. immersing the Chinese wolfberry raw material in water for 1-2 hours; 3. putting the immersed Chinese wolfberry raw material into a pulping machine, and carrying out pulping; 4. carrying out solid-liquid separation; 5. putting the fruit pulp into a circulating ultrasonic extractor, and carrying out primary extraction to obtain a first supernate and a first solid; 6. putting the first solid into the circulating ultrasonic extractor, and carrying out secondary extraction to obtain a second supernate; 7. mixing the first and second supernates, and carrying out concentration; 8. adding 3-5 times by volume of anhydrous ethanol into the concentrated supernate, and carrying out alcohol precipitation; 9. putting the alcohol precipitation solution into an industrial centrifuge, and carrying out separation precipitation; and 10. drying the precipitate to obtain the Chinese wolfberry polysaccharides. The method enhances the purity of the Chinese wolfberry polysaccharides, and has wide application range.
Description
Technical field
The present invention relates to technical field of Chinese medicines, particularly to the extracting method of a kind of lycium barbarum polysaccharide.
Background technology
Fructus Lycii is the rare Chinese medicine that China is traditional, have " red treasured " laudatory title, rich in vegetable polysaccharides, albumen
Matter, vitamins and other nutritious components, its primary efficacy is nourishing the liver and kidney and improving eyesight, for soreness of waist and knee joint, dizziness
Tinnitus, interior-heat Xiao are thirsty, blood deficiency and yellow complexion and the symptom such as blurred vision is not clear.Lycium barbarum polysaccharide is to extract from Fructus Lycii and obtain
A kind of water soluble polysaccharide, this polysaccharide system proteoglycan, molecular weight is 22～25kD, by arabinose, Portugal
Grape sugar, galactose, mannose, xylose, these 6 kinds of monosaccharide components compositions of rhamnose, lycium barbarum polysaccharide is Fructus Lycii
Son regulation immunity, the main active of slow down aging, it is to malignant tumor, the prevention of acquired immune deficiency syndrome (AIDS) and treatment
Also positive role can be played.Additionally it can improve the symptoms such as old people's fatiguability, inappetence and blurred vision,
And there is blood fat reducing, anti-fatty liver, antitumor, the anti-ageing effect of waiting for a long time.Therefore, during lycium barbarum polysaccharide is medicine
Requisite medical material.Existing lycium barbarum polysaccharide extracting method generally includes petroleum ether degreasing wax removing, water-soluble adds
The steps such as heat extraction, ethanol precipitation, centrifugation, drying bu sublimation.But adopt and extract in this way
The purity of lycium barbarum polysaccharide relatively low, range of application is narrow.
Summary of the invention
In view of this, the present invention provides the extracting method of a kind of lycium barbarum polysaccharide, which raises the pure of lycium barbarum polysaccharide
Degree, applied range.
The present invention solves the problems referred to above by techniques below means:
The extracting method of a kind of lycium barbarum polysaccharide of the present invention, comprises the following steps: step 1: remove Fructus Lycii raw material
In impurity；Step 2: the Fructus Lycii raw material after going the removal of impurity soaks 1～2h；Step 3: will soak
After Fructus Lycii raw material put in beater and pull an oar；Step 4: use filter cloth to carry out in the mixture after making beating
Solid-liquid separation so that the pulp in mixture separates with seed of Fructus Lycii；Step 5: pulp is put into circulating ultrasonic and carries
Take and machine carried out extract for the first time, obtain the first extract, and described first extract is carried out solid-liquid separation,
Obtain the first supernatant and the first solids；Step 6: described first solids is put into circulating ultrasonic extractor
In carry out second time extract, obtain the second extract, and described second extract carried out solid-liquid separation, obtain
Second supernatant and the second solids；Step 7: carry out after described first supernatant and the second supernatant are mixed
Concentrate；Step 8: add the dehydrated alcohol of 3～5 times of volumes in supernatant after concentration, in ambient temperature
It is to stand 8～12h at 1.5～7 DEG C to carry out precipitate with ethanol；Step 9: the alcohol deposit fluid after precipitate with ethanol is put into industrial centrifugal
Machine carries out precipitation separation；Step 10: precipitate precipitation obtained is dried, and obtains lycium barbarum polysaccharide.
Further, the Extracting temperature of described circulating ultrasonic extractor be 25～45 DEG C, ultrasonic power be 450～
1800W, circulation stirring rotating speed are 400～2500rpm, extraction time is 0.5～3.5h.
Further, in described step 2 will go the removal of impurity after Fructus Lycii raw material soak 1～2h and specifically include:
1.5h soaked by Fructus Lycii raw material after going the removal of impurity.
Further, the supernatant after concentration in described step 8 adds the dehydrated alcohol of 3～5 times of volumes,
At ambient temperature is 1.5～7 DEG C, stand 8～12h carry out precipitate with ethanol and specifically include: in supernatant after concentration
Add the dehydrated alcohol of 4 times of volumes, at ambient temperature is 5 DEG C, stands 10h carries out precipitate with ethanol.
The extracting method of a kind of lycium barbarum polysaccharide of the present invention has the advantages that
The invention provides the extracting method of a kind of lycium barbarum polysaccharide, Fructus Lycii raw material is carried out successively remove impurity, immersion,
Making beating, then use filter cloth to carry out solid-liquid separation in the mixture after making beating, obtain pulp and the seed of Fructus Lycii separated,
Then pulp is put in circulating ultrasonic extractor and carry out extracting for the first time and after second time extraction, obtaining twice
Supernatant mix, finally carry out mixed supernatant successively concentrating, precipitation separation and dried
To lycium barbarum polysaccharide, owing to said extracted method using twice supersound extraction, so the lycium barbarum polysaccharide obtained is pure
Spend higher, applied range.
Detailed description of the invention
The extracting method of a kind of lycium barbarum polysaccharide of the present invention, mainly comprises the steps that
Step 1: remove the impurity in Fructus Lycii raw material；
Step 2: the Fructus Lycii raw material after going the removal of impurity soaks 1～2h；
Step 3: the Fructus Lycii raw material after soaking is put in beater and pulled an oar；
Step 4: will making beating after mixture use filter cloth carry out solid-liquid separation so that the pulp in mixture with
Seed of Fructus Lycii separates；
Step 5: pulp is put into and carries out in circulating ultrasonic extractor extracting for the first time, obtain the first extract,
And described first extract is carried out solid-liquid separation, obtain the first supernatant and the first solids；
Wherein, the Extracting temperature of described circulating ultrasonic extractor be 25～45 DEG C, ultrasonic power be 450～
1800W, circulation stirring rotating speed are 400～2500rpm, extraction time is 0.5～3.5h.
Step 6: described first solids is put into and carries out second time in circulating ultrasonic extractor and extract, obtain the
Two extracts, and described second extract is carried out solid-liquid separation, obtain the second supernatant and the second solids；
Step 7: concentrate after described first supernatant and the second supernatant are mixed；
Step 8: add the dehydrated alcohol of 3～5 times of volumes in supernatant after concentration, in ambient temperature be
Stand 8～12h at 1.5～7 DEG C and carry out precipitate with ethanol；
Step 9: the alcohol deposit fluid after precipitate with ethanol is put in industrial centrifugal machine and carry out precipitation separation；
Step 10: precipitate precipitation obtained is dried, and obtains lycium barbarum polysaccharide.
The invention provides the extracting method of a kind of lycium barbarum polysaccharide, Fructus Lycii raw material is carried out successively remove impurity, immersion,
Making beating, then use filter cloth to carry out solid-liquid separation in the mixture after making beating, obtain pulp and the seed of Fructus Lycii separated,
Then pulp is put in circulating ultrasonic extractor and carry out extracting for the first time and after second time extraction, obtaining twice
Supernatant mix, finally carry out mixed supernatant successively concentrating, precipitation separation and dried
To lycium barbarum polysaccharide, owing to said extracted method using twice supersound extraction, so the lycium barbarum polysaccharide obtained is pure
Spend higher, applied range.
Embodiment one
Step 1: remove the impurity in Fructus Lycii raw material.
Step 2: 1.5h soaked by the Fructus Lycii raw material after going the removal of impurity.
Step 3: the Fructus Lycii raw material after soaking is put in beater and pulled an oar.
Step 4: will making beating after mixture use filter cloth carry out solid-liquid separation so that the pulp in mixture with
Seed of Fructus Lycii separates.
Step 5: pulp is put into and carries out in circulating ultrasonic extractor extracting for the first time, Extracting temperature is 25 DEG C,
Ultrasonic power is 450W, circulation stirring rotating speed is 400rpm, extraction time is 0.5h, obtains the first extract,
And described first extract is carried out solid-liquid separation, obtain the first supernatant and the first solids.
Step 6: described first solids is put into and carries out in circulating ultrasonic extractor extracting for the second time, extract temperature
Degree is 35 DEG C, ultrasonic power is 1200W, circulation stirring rotating speed is 1200rpm, extraction time is 2h, obtains
Second extract, and described second extract is carried out solid-liquid separation, obtain the second supernatant and the second solids.
Step 7: concentrate after described first supernatant and the second supernatant are mixed.
Step 8: add the dehydrated alcohol of 4 times of volumes in supernatant after concentration, be 5 DEG C in ambient temperature
Lower standing 10h carries out precipitate with ethanol.
Step 9: the alcohol deposit fluid after precipitate with ethanol is put in industrial centrifugal machine and carry out precipitation separation.
Step 10: precipitate precipitation obtained is dried, and obtains lycium barbarum polysaccharide.
Detecting the lycium barbarum polysaccharide obtained, testing result is as follows:
It is 85% by the content of lycium barbarum polysaccharide in the extract that embodiment one obtains.
Embodiment two
Step 1: remove the impurity in Fructus Lycii raw material.
Step 2: 2h soaked by the Fructus Lycii raw material after going the removal of impurity.
Step 3: the Fructus Lycii raw material after soaking is put in beater and pulled an oar.
Step 4: will making beating after mixture use filter cloth carry out solid-liquid separation so that the pulp in mixture with
Seed of Fructus Lycii separates.
Step 5: pulp is put into and carries out in circulating ultrasonic extractor extracting for the first time, Extracting temperature is 40 DEG C,
Ultrasonic power is 1500W, circulation stirring rotating speed is 1800rpm, extraction time is 2.8h, obtains the first extraction
Thing, and described first extract is carried out solid-liquid separation, obtain the first supernatant and the first solids.
Step 6: described first solids is put into and carries out in circulating ultrasonic extractor extracting for the second time, extract temperature
Degree is 45 DEG C, ultrasonic power is 1800W, circulation stirring rotating speed is 2500rpm, extraction time is 3.5h,
To the second extract, and described second extract is carried out solid-liquid separation, obtain the second supernatant and the second solid
Thing.
Step 7: concentrate after described first supernatant and the second supernatant are mixed.
Step 8: add the dehydrated alcohol of 5 times of volumes in supernatant after concentration, be 7 DEG C in ambient temperature
Lower standing 12h carries out precipitate with ethanol.
Step 9: the alcohol deposit fluid after precipitate with ethanol is put in industrial centrifugal machine and carry out precipitation separation.
Step 10: precipitate precipitation obtained is dried, and obtains lycium barbarum polysaccharide.
Detecting the lycium barbarum polysaccharide obtained, testing result is as follows:
It is 90% by the content of lycium barbarum polysaccharide in the extract that embodiment two obtains.
Finally illustrating, above example is only in order to illustrate technical scheme and unrestricted, although ginseng
According to preferred embodiment, the present invention is described in detail, it will be understood by those within the art that, permissible
Technical scheme is modified or equivalent, without deviating from technical solution of the present invention objective and
Scope, it all should be contained in the middle of scope of the presently claimed invention.
Claims (4)
1. the extracting method of a lycium barbarum polysaccharide, it is characterised in that: comprise the following steps:
Step 1: remove the impurity in Fructus Lycii raw material；
Step 2: the Fructus Lycii raw material after going the removal of impurity soaks 1～2h；
Step 3: the Fructus Lycii raw material after soaking is put in beater and pulled an oar；
Step 4: will making beating after mixture use filter cloth carry out solid-liquid separation so that the pulp in mixture with
Seed of Fructus Lycii separates；
Step 5: pulp is put into and carries out in circulating ultrasonic extractor extracting for the first time, obtain the first extract,
And described first extract is carried out solid-liquid separation, obtain the first supernatant and the first solids；
Step 6: described first solids is put into and carries out second time in circulating ultrasonic extractor and extract, obtain the
Two extracts, and described second extract is carried out solid-liquid separation, obtain the second supernatant and the second solids；
Step 7: concentrate after described first supernatant and the second supernatant are mixed；
Step 8: add the dehydrated alcohol of 3～5 times of volumes in supernatant after concentration, in ambient temperature be
Stand 8～12h at 1.5～7 DEG C and carry out precipitate with ethanol；
Step 9: the alcohol deposit fluid after precipitate with ethanol is put in industrial centrifugal machine and carry out precipitation separation；
Step 10: precipitate precipitation obtained is dried, and obtains lycium barbarum polysaccharide.
The extracting method of a kind of lycium barbarum polysaccharide the most according to claim 1, it is characterised in that follow described in:
The Extracting temperature of ring ultrasonic extractor is 25～45 DEG C, ultrasonic power is 450～1800W, circulation stirring rotating speed
Be 400～2500rpm, extraction time be 0.5～3.5h.
The extracting method of a kind of lycium barbarum polysaccharide the most according to claim 2, it is characterised in that: described step
In rapid 2 will go the removal of impurity after Fructus Lycii raw material soak 1～2h and specifically include:
1.5h soaked by Fructus Lycii raw material after going the removal of impurity.
The extracting method of a kind of lycium barbarum polysaccharide the most according to claim 3, it is characterised in that: described step
In supernatant after concentration in rapid 8 add 3～5 times of volumes dehydrated alcohol, ambient temperature be 1.5～
Stand 8～12h at 7 DEG C to carry out precipitate with ethanol and specifically include:
Supernatant after concentration adds the dehydrated alcohol of 4 times of volumes, at ambient temperature is 5 DEG C, stands 10h
Carry out precipitate with ethanol.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610570660.2A CN105968224A (en) | 2016-07-19 | 2016-07-19 | Extraction method of Chinese wolfberry polysaccharides |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610570660.2A CN105968224A (en) | 2016-07-19 | 2016-07-19 | Extraction method of Chinese wolfberry polysaccharides |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105968224A true CN105968224A (en) | 2016-09-28 |
Family
ID=56952829
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610570660.2A Pending CN105968224A (en) | 2016-07-19 | 2016-07-19 | Extraction method of Chinese wolfberry polysaccharides |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105968224A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106834383A (en) * | 2017-02-21 | 2017-06-13 | 中国科学院过程工程研究所 | A kind of method for lifting LBP-X quality and its application |
| CN107198240A (en) * | 2017-07-12 | 2017-09-26 | 青岛大学 | The combined extraction method of plurality of active ingredients in black fruit fructus lycii |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103788228A (en) * | 2014-02-17 | 2014-05-14 | 银川泰丰生物科技有限公司 | Preparation method of lycium barbarum polysaccharide |
| CN104059163A (en) * | 2014-07-08 | 2014-09-24 | 中国科学院西北高原生物研究所 | Novel method for preparing Qinghai Qaidam lycium barbarum polysaccharide in extraction and separation manner |
-
2016
- 2016-07-19 CN CN201610570660.2A patent/CN105968224A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103788228A (en) * | 2014-02-17 | 2014-05-14 | 银川泰丰生物科技有限公司 | Preparation method of lycium barbarum polysaccharide |
| CN104059163A (en) * | 2014-07-08 | 2014-09-24 | 中国科学院西北高原生物研究所 | Novel method for preparing Qinghai Qaidam lycium barbarum polysaccharide in extraction and separation manner |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106834383A (en) * | 2017-02-21 | 2017-06-13 | 中国科学院过程工程研究所 | A kind of method for lifting LBP-X quality and its application |
| CN106834383B (en) * | 2017-02-21 | 2021-04-20 | 北京同仁堂健康药业(宁夏)有限公司 | Method for improving quality of lycium barbarum polysaccharide and application thereof |
| CN107198240A (en) * | 2017-07-12 | 2017-09-26 | 青岛大学 | The combined extraction method of plurality of active ingredients in black fruit fructus lycii |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105925368B (en) | A method of walnut oil is prepared by water law demulsification walnut oil body | |
| CN101348529B (en) | Extraction and separation method of medlar polysaccharide | |
| JP2009528847A5 (en) | ||
| CN103462010B (en) | Lycium ruthenicum oral solution and production method thereof | |
| CN103642592A (en) | Full utilization technology of rose flower | |
| CN104059163B (en) | Qaidam, Qinghai lycium barbarum polysaccharide is extracted and is separated novel preparation method | |
| CN105039476A (en) | Method for preparing black bean antioxidant polypepetide powder through compound enzyme method | |
| CN109762651A (en) | A kind of efficient preparation process of high-quality pecan oil | |
| CN105693680B (en) | A kind of extracting method of indigo fruit anthocyanidin | |
| CN105038947A (en) | Supercritical extraction method of walnut oil | |
| CN104592412A (en) | Method for extracting chestnut polysaccharide | |
| CN105968224A (en) | Extraction method of Chinese wolfberry polysaccharides | |
| CN104387498B (en) | Technical method for simultaneously preparing premna microphylla pectin and premna microphylla sodium copper chlorophyll from premna microphylla | |
| CN106070863A (en) | A kind of Pseudobulbus Bletillae (Rhizoma Bletillae) scented tea and preparation method thereof | |
| CN104642564A (en) | Sesame oil with whitening effect and preparation method thereof | |
| CN103980372A (en) | Method for concurrently preparing low viscosity semen plantaginis polysaccharide and high viscosity semen plantaginis polysaccharide | |
| CN103772525A (en) | Method for extracting dragon fruit flower polysaccharide | |
| CN105732831A (en) | Microwave-assisted auricularia auriculajudae polysaccharide extraction preparation method | |
| CN107125372A (en) | The preparation method of Ilex Latifolia Thunb leaf of Moringa composite instant tea powder | |
| CN106281636A (en) | A kind of extraction camellia seed oil and method of tea saponin from tea-seed pancake | |
| CN106720801B (en) | Burdock tea rich in inulin | |
| CN105601948B (en) | A kind of method that plantago asiatica linne polyoses viscosity is reduced using tea polyphenols | |
| CN111233971B (en) | Method for extracting plant protein isolate | |
| CN103976357B (en) | A kind of red date Calophyllum Inophyllum L composite nourishing dense liquid production technology | |
| CN105837547A (en) | Method for separating procyanidin polymer and oligomer from degreased sea buckthorn seeds |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160928 |