CN1059568C - Tech. for extracting nutrient and substance of lowering blood sugar from crassula portulacea a cactus plant - Google Patents
Tech. for extracting nutrient and substance of lowering blood sugar from crassula portulacea a cactus plant Download PDFInfo
- Publication number
- CN1059568C CN1059568C CN95117499A CN95117499A CN1059568C CN 1059568 C CN1059568 C CN 1059568C CN 95117499 A CN95117499 A CN 95117499A CN 95117499 A CN95117499 A CN 95117499A CN 1059568 C CN1059568 C CN 1059568C
- Authority
- CN
- China
- Prior art keywords
- crassula
- former powder
- technology
- swallow
- palm
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000000126 substance Substances 0.000 title abstract description 5
- 239000008280 blood Substances 0.000 title description 10
- 210000004369 blood Anatomy 0.000 title description 10
- 241000219357 Cactaceae Species 0.000 title 1
- 241000304944 Crassula portulacea Species 0.000 title 1
- 235000015097 nutrients Nutrition 0.000 title 1
- 239000000843 powder Substances 0.000 claims abstract description 23
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 18
- 238000000034 method Methods 0.000 claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 5
- 241001660917 Crassula ovata Species 0.000 claims description 15
- 238000001914 filtration Methods 0.000 claims description 9
- 230000008569 process Effects 0.000 claims description 6
- 239000000706 filtrate Substances 0.000 claims description 5
- 238000001704 evaporation Methods 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 2
- 238000003825 pressing Methods 0.000 claims description 2
- 238000009775 high-speed stirring Methods 0.000 claims 1
- 239000002893 slag Substances 0.000 claims 1
- 238000005516 engineering process Methods 0.000 abstract description 17
- 238000004519 manufacturing process Methods 0.000 abstract description 12
- 210000002421 cell wall Anatomy 0.000 abstract description 9
- 238000007710 freezing Methods 0.000 abstract description 8
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 abstract description 6
- 239000002360 explosive Substances 0.000 abstract description 3
- 230000008014 freezing Effects 0.000 abstract description 3
- 239000003208 petroleum Substances 0.000 abstract description 3
- 241000220284 Crassulaceae Species 0.000 abstract description 2
- 230000000050 nutritive effect Effects 0.000 abstract description 2
- 238000012360 testing method Methods 0.000 description 9
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 5
- 239000008103 glucose Substances 0.000 description 5
- 230000004044 response Effects 0.000 description 5
- 235000013619 trace mineral Nutrition 0.000 description 5
- 239000011573 trace mineral Substances 0.000 description 5
- 235000016425 Arthrospira platensis Nutrition 0.000 description 4
- 240000002900 Arthrospira platensis Species 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 4
- ZNNLBTZKUZBEKO-UHFFFAOYSA-N glyburide Chemical compound COC1=CC=C(Cl)C=C1C(=O)NCCC1=CC=C(S(=O)(=O)NC(=O)NC2CCCCC2)C=C1 ZNNLBTZKUZBEKO-UHFFFAOYSA-N 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 229940082787 spirulina Drugs 0.000 description 4
- 235000001167 Melaleuca cajuputi Nutrition 0.000 description 3
- 244000304222 Melaleuca cajuputi Species 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000013641 positive control Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 238000011084 recovery Methods 0.000 description 3
- 208000007271 Substance Withdrawal Syndrome Diseases 0.000 description 2
- 230000000857 drug effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 229960004580 glibenclamide Drugs 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 239000000575 pesticide Substances 0.000 description 2
- 238000000053 physical method Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000003252 repetitive effect Effects 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 238000010257 thawing Methods 0.000 description 2
- 229910017518 Cu Zn Inorganic materials 0.000 description 1
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
- 241000700157 Rattus norvegicus Species 0.000 description 1
- 229940127003 anti-diabetic drug Drugs 0.000 description 1
- 239000003472 antidiabetic agent Substances 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
Images
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to important innovation of a production technology that swallow palm initial powder are extracted from crassulaceae plants. The original production technology which is only suitable for labs requires a large amount of inflammable and explosive ethanol and petroleum ether or high temperature and high pressure in the production process, so the original production technology enlarges the cost, is not safe, loses a lot of nutritive substances, has low yield, etc. The new technology adopts ethanol of low concentration and ultrasonic wave, and breaks cell walls in a method of quick freezing and unfreezing; at the normal temperature, the swallow palm initial powder is extracted with water for many times; thus, the technology is simplified, and the quality and the yield of the swallow palm initial powder are increased.
Description
Crassula argentea Thunb, cajaput belong to Crassulaceae, are ornamental plant, have more wild Crassula argentea Thunb, cajaput to distribute in Guangxi China crust horse mountain area, but the main source of raw materials for production are by artificial culture.
The present invention relates to the Chinese medicine extraction method on the medical industry.
Originally produce the technology of the former powder of Crassula argentea Thunb, be to get according to the method for Japan special permission communique 5-19554, lay a good foundation for from natural plants, finding new high protein and efficient blood glucose material, but it is long that this method has run into technological process in process of production, complicated operation, inflammable and explosive ethanol and the petroleum ether of a large amount of uses, the production cost height, problems such as the product poorly water-soluble and the response rate are low, in order to address these problems, in Japan and domesticly carried out a series of further investigation and in production practices, carried out repetition test, proposed to be divided into the new production technology of two classes by chemistry and physical method breaking cell wall.
The characteristics of new technology: 1. cut down inflammable, explosive petroleum ether; 2. reduce alcohol concentration and make it nonflammable in process of production, keep the effect of the broken plant cell wall of ethanol simultaneously from 49%-5%; 3. leaching process is to carry out under room temperature 5-35 ℃, to guarantee the physiologically active of its effective ingredient; 4. simplified with the proteinic operation of 70% alcohol precipitation, kept high protein and trace element and the bioactive substance thereof in the plant; 5. product quality and yield have been improved than old technology.The former powder elaboration of producing with new technology of Crassula argentea Thunb carries out the test of pesticide effectiveness referring to table 3, and is respond well.
1, chemical method breaking cell wall embodiment sees Table the 1-10 example of enumerating in 1.
Embodiment 1 operates by the new technological flow figure of Fig. 1 .49%-5% ethanol breaking cell wall: stem, the leaf of bright Crassula argentea Thunb of 100kg or cajaput are cleaned, placed pulper for example, 49% the medicinal alcohol that adds 50 liters again, soaked 4 hours, high-speed stirred is homogenate, filters.Filtering residue is pressed above-mentioned repetitive operation 3 times with 50 premium on currency respectively, merge 4 times filtrate, be evaporated to behind the recovered alcohol and be thick, put into evaporating pan and be placed on far-infrared oven, dry at 40-50 ℃,, get the former powder of elaboration Crassula argentea Thunb (hereinafter to be referred as the former powder of DSP) 2.4kg with crossing 60 mesh sieves after the pulverizer fragmentation, with the Crassula argentea Thunb fresh weight is that benchmark calculates, yield is 2.4% (original production technology yield: 1.1-2.33%; Average yield is 1.59%).
Embodiment 2-10 gives an example: press the technology of Fig. 1, and operation the same (summary), its alcohol concentration yield is as shown in table 1.
Table 1.
Embodiment 11. filtering residues reclaim to be operated by the recovery artwork of Fig. 2 Crassula argentea Thunb filtering residue for example: the 4.1kg filtering residue is soaked into 50 premium on currency, and 24 hours post-heating to 50 ℃ are cooled to room temperature, filter pressing, and filtering residue is pressed above-mentioned repetitive operation 4 times, merges 5 times filtrate.50-70 ℃ of concentrating under reduced pressure is concentrated into slightly thickly, puts into evaporating pan, is placed in the far-infrared oven again, do 40-50 ℃ of baking, 60 orders of pulverizing, sieve, elaboration 0.05kg, the response rate is 0.05%.
| Project implementation example numbering | Crassula argentea Thunb fresh weight (kg) | Alcohol concentration | Output (kg) | Yield (%) | The response rate (wt%) | Total recovery (wt%) |
| 1 | 100 | 49％ | 2.4 | 2.4 | 0.05 | 2.45 |
| 2 | 100 | 45％ | 2.35 | 2.35 | 0.035 | 2.39 |
| 3 | 100 | 40％ | 2.41 | 2.41 | 0.07 | 2.48 |
| 4 | 100 | 35％ | 2.5 | 2.5 | 0.12 | 2.62 |
| 5 | 100 | 30％ | 2.45 | 2.45 | 0.09 | 2.54 |
| 6 | 100 | 25％ | 2.38 | 2.38 | 0.19 | 2.57 |
| 7 | 100 | 20％ | 2.28 | 2.29 | 0.22 | 2.51 |
| 8 | 100 | 15％ | 2.30 | 2.30 | 0.18 | 2.48 |
| 9 | 100 | 10％ | 2.39 | 2.39 | 0.085 | 2.48 |
| 10 | 100 | 5％ | 2.27 | 2.27 | 0.04 | 2.31 |
The response rate saw Table shown in 1. embodiment during the filtering residue of embodiment 2-10 reclaimed for example.The filtering residue of embodiment 12. embodiment 2-10 reclaims the same for example (summary)
2, physical method breaking cell wall embodiment:
1. quick-freezing freezing process: operational approach is pressed Fig. 3 quick-freezing speed thawing technique flow operations, quick-freezing speed thaw point scope, and yield sees table 2 for details.
Table 2
*-5 ℃ refer to quick freezing temperature, about 24 hours of quick-freezing time, thaw and need heat rapidly to 50 ℃.
2. ultrasonic disruption cell wall embodiment
Embodiment: the new technological flow figure by Fig. 4 ultrasound wave breaking cell wall operates, getting the fresh Crassula argentea Thunb of 100kg cleans, add 50 premium on currency, smash to pieces with bruisher and to be homogenate,, filter with ultrasonic stirring 2 hours, repeat aforesaid operations 3 times, merge 4 times filtrate, the filtering residue response rate is 0.08%, the former powder yield 2.56kg of DSP.Yield: 2.56%.Total recovery: 2.64%.
The test of pesticide effectiveness:
1. the former powder hypoglycemic activity of DSP effect observation test: experiment material is the female Wistar rat, body weight 230-260g, and test method sees the special permission communique for details puts down into 5-19554, repeats no more here, and result of the test is as shown in table 3.The former powder of DSP is from example 10 sampling from table of table 1.
Table 3 is respectively organized blood glucose mean variation situation (mg/dl M ± SD)
* with before the administration compare P＜0.05 * * P＜0.01 △ and compare P＜0.05 △ △ P＜0.01 with positive control
| Group | Number of animals | Blood glucose value | |||||
| Before the administration | After the administration | ||||||
| 2 days | 4 days | 6 days | 8 days | 14 days | |||
| The former powder 20mg/kg of DSP | 11 | 347.0 (110.54) | 256.3△ (108.10) | 282.7△ (77.39) | 226.6 *△ (83.40) | 250.7 *△ (63.08) | 154.1 **△△ (30.48) |
| Glyburide 1.6mg/kg | 10 | 339.4 (119.65) | 280.4△ (112.61) | 300.1 (75.86) | 234.1△ (92.52) | 283.5 (133.21) | 243.2 (95.70) |
| Positive control | 11 | 323.6 (144.21) | 431.6 (182.93) | 410.0 (165.09) | 370.9 (137.26) | 416.8 (182.72) | 383.8 (199.98) |
Check through the t value, compare before each day after positive controls and the administration of glyburide group and the administration and find no statistical significance and difference (P＞0.05), and 5g/kg is organized in the 6th day and begins to have significance difference (P＜0.05), still has the difference (P＜0.01) of highly significant to compare with positive group after the drug withdrawal in 6 days: the 5g/kg group all promptly has remarkable reduction (P＜0.05) from the 2nd day beginning blood glucose; The glyburide group also had significant difference (P＜0.05) at 2 days and 5 days.
This shows that the former powder of DSP (fresh weight Crassula argentea Thunb extracting solution is dried to powder) acting duration is longer, drug withdrawal still can be kept blood sugar reducing function after 6 days.Thereby its blood sugar reducing function is better than glyburide.
The animal experiment of the former powder of DSP: method and material are the same, and result of the test is as shown in table 4
The former powder animal experiment of table 4 DSP
* be the sampling among the embodiment 2 of thawing of quick-freezing speed; * takes a sample among the ultrasonic disruption cell wall embodiment
| Group | Number of animals | Sex | Blood sugar content mg% | Blood glucose rate of descent (Δ X) (%) | The P value | ||
| Before the administration | After the administration | -ΔX±SD | |||||
| The former powder 20mg/kg of DSP * | 11 | ♀ | 198.41 | 146.52 | 51.89±40.99 | 51.89 | ＜0.01 |
| The former powder 20mg/kg of DSP ** | 11 | ♀ | 192.35 | 149.45 | 42.9±39.3 | 42.9 | ＜0.01 |
| Matched group | 11 | ♀ | 180.67 | 179.98 | 3.69±5.94 | 3.69 | —— |
3, the effective ingredient of new, old production technology and function ratio see Table 5.
Table 5
| Project construction technology | Drug effect (P value) | Total protein content % | Total sugar content % | Water solublity | Average yield |
| Old technology | ＜0.01 | 3-4.5 | ＜1 | Be partly dissolved | 1.58 |
| New technology | ＜0.01 | 30-60 | 4-6 | Molten entirely | 2.48 |
4, the mensuration of the albumen in the former powder of DSP, sugar, trace element etc.:
It is as shown in table 6 to press Chinese Pharmacopoeia method measurement result
1. table 6 albumen, sugar, water content test result
| The mensuration project | Content range | Peak | Minimum | Primary standard substance | Test instrunment |
| The total sugar amount | 4-6.0％ | 6％ | 4％ | D-mannose glutamer 200 | Ultraviolet-visible spectrophotometer-340 type, the differential thermal thermogravimetric analyzer |
| Protein content | 30-60％ | 60％ | 30％ | ||
| Water content | 5-8％ | 8％ | 5％ |
2. trace elements determination: measure with ICP (electron spectrum), Britain, test result is as shown in table 7.
Table 7
| Element term | Mg | Al | Fe | P | Cu | Zn | Ca | Pb | Cd | Cr | K + | Cl - |
| Content (mg/kg) | 20.3 | 0.2 | 0.48 | 10.3 | 0.03 | 0.17 | 62.2 | ＜1 | ＜1 | ＜1 | 55.0 | 68.0 |
| Percentage composition (%) | 2.03 | 0.02 | 0.048 | 1.03 | 0.003 | 0.017 | 6.22 | ＜0.1 | ＜0.1 | ＜0.1 | 5.5 | 6.8 |
5, the comparison of former powder of DSP and nutritive component of spirulina: as shown in table 8
Table 8
| Project content (%) title | Protein content % | Total sugar amount % | Trace element % |
| The former powder of DSP | 30-60 | 4-6 | ＞9 |
| Spirulina (dry weight) | 40-70 | 12-18 | 5-8 |
From table 3 to table 8 as seen, the drug effect of the former powder of DSP has the wide development prospect, and from the angle of nourishing healthy, albumen, sugar are all near spirulina, and trace element exceeds spirulina, and is very big as natural nutrition health food, health product, antidiabetic drug potentiality to be exploited.
Claims (2)
1, the extracting method of the former powder of Crassula argentea Thunb, comprise the stem and the leaf of bright Crassula argentea Thunb are cleaned, blend, the medicinal alcohol that adds the 49-5% of 0.5 times of amount soaked 4 hours, high-speed stirring mixes homogenate, filter, behind the filtrate recovered alcohol, be evaporated to and be thick and in 40-50 ℃ of infrared baking box, dry, pulverize and obtain the former powder of Crassula argentea Thunb.
2, will filter out the Lu slag according to the process of claim 1 wherein, be soaked in the water, after 24 hours, be heated to 50 ℃, be cooled to room temperature, filter pressing, filtering residue is pressed aforesaid operations and is repeated 4 times, merges 5 times filtrate.50-70 ℃ is evaporated to slightly thickly, puts into evaporating pan, is placed in the far ultraviolet baking box again, does 40-50 ℃ of baking, pulverizes, and crosses 60 mesh sieves, elaboration.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN95117499A CN1059568C (en) | 1995-11-24 | 1995-11-24 | Tech. for extracting nutrient and substance of lowering blood sugar from crassula portulacea a cactus plant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN95117499A CN1059568C (en) | 1995-11-24 | 1995-11-24 | Tech. for extracting nutrient and substance of lowering blood sugar from crassula portulacea a cactus plant |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1151303A CN1151303A (en) | 1997-06-11 |
| CN1059568C true CN1059568C (en) | 2000-12-20 |
Family
ID=5081285
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN95117499A Expired - Lifetime CN1059568C (en) | 1995-11-24 | 1995-11-24 | Tech. for extracting nutrient and substance of lowering blood sugar from crassula portulacea a cactus plant |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1059568C (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03106819A (en) * | 1989-09-19 | 1991-05-07 | Kei Cho | Hypoglycemic drug |
-
1995
- 1995-11-24 CN CN95117499A patent/CN1059568C/en not_active Expired - Lifetime
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03106819A (en) * | 1989-09-19 | 1991-05-07 | Kei Cho | Hypoglycemic drug |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1151303A (en) | 1997-06-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101911956B (en) | Plant source pesticide and production method and application thereof | |
| CN1283266C (en) | Preparation and application of nanometer chrysalid worm grass meal | |
| CN1059568C (en) | Tech. for extracting nutrient and substance of lowering blood sugar from crassula portulacea a cactus plant | |
| Maltseva et al. | Investigation of the influence of the properties of the pressed material on the energy consumption and design parameters of the oil press | |
| CN112806569A (en) | Preparation method and application of composition with functions of protecting gastric mucosa and resisting helicobacter pylori | |
| CN116369382A (en) | Plant polypeptide nutrition preparation for promoting teenager development and growth and preparation method thereof | |
| CN113249227B (en) | Novel lucid ganoderma strain and cultivation application thereof based on medicinal residue fungus bag | |
| CN108013469A (en) | A kind of preparation method of auxiliary hyperglycemic balsam pear tablet | |
| CN107693568A (en) | A kind of preparation method of defatted MOLEPLANT SEED | |
| DE69713078T2 (en) | LINSEED DRUG | |
| CN108782613A (en) | Camellia oleifera Abel dreg extract and biopesticide prepared from same | |
| JP3097997B2 (en) | Pure natural blood sugar lowering agent | |
| CN1147553C (en) | Working technology for extracting ossein from animal cartilage | |
| CN110960585B (en) | Superfine powder dispersible tablet of active ingredients of Chinese date and preparation method thereof | |
| JP2001069921A (en) | Health food for pet animal and livestock | |
| CN103734593B (en) | Production process of healthy food compound American ginseng rice | |
| CN109221331A (en) | With the long-acting emerald enzyme heart cake and preparation method thereof of reducing weight and beautifying features | |
| JPH08221A (en) | Production of dietary fiber powder from oil palm trunk and functional food | |
| CN108704022A (en) | A kind of medical, edible Exocarpium Citri Grandis ingredient preparation method and its preparation | |
| KR100586326B1 (en) | Mass culture of myceliummushroom using Opuntia ficus-indica var. saboten as a substrate and the produced myceliummushroom | |
| CN1225987C (en) | Green pure plant insecticide | |
| KR100211287B1 (en) | Processing method of pollen | |
| CN117598466B (en) | Turmeric nano-emulsion freeze-dried powder, curry powder and preparation method thereof | |
| Kubal et al. | Pharmaceutical approach of Taro (Colocasia esculenta) | |
| JPS60146828A (en) | Inhibitor for rise in cholesterol and health food containing said inhibitor |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C06 | Publication | ||
| PB01 | Publication | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee |
Owner name: BEIJING KINGSUN-AIMA TECHNOLOGY CO., LTD. Free format text: FORMER NAME OR ADDRESS: DING JIAN |
|
| CP03 | Change of name, title or address |
Address after: No. 48, building A, building 1, Beijing science and Technology Exhibition Center, No. 3 West Third Ring Road, Beijing, Haidian District, 9C Patentee after: Junyang-Aima Science-Technology Co., Ltd., Beijing Address before: Room 108, building 404, Ze Ze garden, Peking University, Beijing Patentee before: Ding Jian |
|
| DD01 | Delivery of document by public notice |
Addressee: Junyang-Aima Science-Technology Co., Ltd., Beijing Document name: Notification to Pay the Fees |
|
| CX01 | Expiry of patent term |
Granted publication date: 20001220 |
|
| EXPY | Termination of patent right or utility model |