CN1059568C - Tech. for extracting nutrient and substance of lowering blood sugar from crassula portulacea a cactus plant - Google Patents
Tech. for extracting nutrient and substance of lowering blood sugar from crassula portulacea a cactus plant Download PDFInfo
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- CN1059568C CN1059568C CN95117499A CN95117499A CN1059568C CN 1059568 C CN1059568 C CN 1059568C CN 95117499 A CN95117499 A CN 95117499A CN 95117499 A CN95117499 A CN 95117499A CN 1059568 C CN1059568 C CN 1059568C
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Abstract
The present invention relates to important innovation of a production technology that swallow palm initial powder are extracted from crassulaceae plants. The original production technology which is only suitable for labs requires a large amount of inflammable and explosive ethanol and petroleum ether or high temperature and high pressure in the production process, so the original production technology enlarges the cost, is not safe, loses a lot of nutritive substances, has low yield, etc. The new technology adopts ethanol of low concentration and ultrasonic wave, and breaks cell walls in a method of quick freezing and unfreezing; at the normal temperature, the swallow palm initial powder is extracted with water for many times; thus, the technology is simplified, and the quality and the yield of the swallow palm initial powder are increased.
Description
Crassula argentea Thunb, cajaput belong to Crassulaceae, are ornamental plant, have more wild Crassula argentea Thunb, cajaput to distribute in Guangxi China crust horse mountain area, but the main source of raw materials for production are by artificial culture.
The present invention relates to the Chinese medicine extraction method on the medical industry.
Originally produce the technology of the former powder of Crassula argentea Thunb, be to get according to the method for Japan special permission communique 5-19554, lay a good foundation for from natural plants, finding new high protein and efficient blood glucose material, but it is long that this method has run into technological process in process of production, complicated operation, inflammable and explosive ethanol and the petroleum ether of a large amount of uses, the production cost height, problems such as the product poorly water-soluble and the response rate are low, in order to address these problems, in Japan and domesticly carried out a series of further investigation and in production practices, carried out repetition test, proposed to be divided into the new production technology of two classes by chemistry and physical method breaking cell wall.
The characteristics of new technology: 1. cut down inflammable, explosive petroleum ether; 2. reduce alcohol concentration and make it nonflammable in process of production, keep the effect of the broken plant cell wall of ethanol simultaneously from 49%-5%; 3. leaching process is to carry out under room temperature 5-35 ℃, to guarantee the physiologically active of its effective ingredient; 4. simplified with the proteinic operation of 70% alcohol precipitation, kept high protein and trace element and the bioactive substance thereof in the plant; 5. product quality and yield have been improved than old technology.The former powder elaboration of producing with new technology of Crassula argentea Thunb carries out the test of pesticide effectiveness referring to table 3, and is respond well.
1, chemical method breaking cell wall embodiment sees Table the 1-10 example of enumerating in 1.
Embodiment 1 operates by the new technological flow figure of Fig. 1 .49%-5% ethanol breaking cell wall: stem, the leaf of bright Crassula argentea Thunb of 100kg or cajaput are cleaned, placed pulper for example, 49% the medicinal alcohol that adds 50 liters again, soaked 4 hours, high-speed stirred is homogenate, filters.Filtering residue is pressed above-mentioned repetitive operation 3 times with 50 premium on currency respectively, merge 4 times filtrate, be evaporated to behind the recovered alcohol and be thick, put into evaporating pan and be placed on far-infrared oven, dry at 40-50 ℃,, get the former powder of elaboration Crassula argentea Thunb (hereinafter to be referred as the former powder of DSP) 2.4kg with crossing 60 mesh sieves after the pulverizer fragmentation, with the Crassula argentea Thunb fresh weight is that benchmark calculates, yield is 2.4% (original production technology yield: 1.1-2.33%; Average yield is 1.59%).
Embodiment 2-10 gives an example: press the technology of Fig. 1, and operation the same (summary), its alcohol concentration yield is as shown in table 1.
Table 1.
Embodiment 11. filtering residues reclaim to be operated by the recovery artwork of Fig. 2 Crassula argentea Thunb filtering residue for example: the 4.1kg filtering residue is soaked into 50 premium on currency, and 24 hours post-heating to 50 ℃ are cooled to room temperature, filter pressing, and filtering residue is pressed above-mentioned repetitive operation 4 times, merges 5 times filtrate.50-70 ℃ of concentrating under reduced pressure is concentrated into slightly thickly, puts into evaporating pan, is placed in the far-infrared oven again, do 40-50 ℃ of baking, 60 orders of pulverizing, sieve, elaboration 0.05kg, the response rate is 0.05%.
| Project implementation example numbering | Crassula argentea Thunb fresh weight (kg) | Alcohol concentration | Output (kg) | Yield (%) | The response rate (wt%) | Total recovery (wt%) |
| 1 | 100 | 49％ | 2.4 | 2.4 | 0.05 | 2.45 |
| 2 | 100 | 45％ | 2.35 | 2.35 | 0.035 | 2.39 |
| 3 | 100 | 40％ | 2.41 | 2.41 | 0.07 | 2.48 |
| 4 | 100 | 35％ | 2.5 | 2.5 | 0.12 | 2.62 |
| 5 | 100 | 30％ | 2.45 | 2.45 | 0.09 | 2.54 |
| 6 | 100 | 25％ | 2.38 | 2.38 | 0.19 | 2.57 |
| 7 | 100 | 20％ | 2.28 | 2.29 | 0.22 | 2.51 |
| 8 | 100 | 15％ | 2.30 | 2.30 | 0.18 | 2.48 |
| 9 | 100 | 10％ | 2.39 | 2.39 | 0.085 | 2.48 |
| 10 | 100 | 5％ | 2.27 | 2.27 | 0.04 | 2.31 |
The response rate saw Table shown in 1. embodiment during the filtering residue of embodiment 2-10 reclaimed for example.The filtering residue of embodiment 12. embodiment 2-10 reclaims the same for example (summary)
2, physical method breaking cell wall embodiment:
1. quick-freezing freezing process: operational approach is pressed Fig. 3 quick-freezing speed thawing technique flow operations, quick-freezing speed thaw point scope, and yield sees table 2 for details.
Table 2
*-5 ℃ refer to quick freezing temperature, about 24 hours of quick-freezing time, thaw and need heat rapidly to 50 ℃.
2. ultrasonic disruption cell wall embodiment
Embodiment: the new technological flow figure by Fig. 4 ultrasound wave breaking cell wall operates, getting the fresh Crassula argentea Thunb of 100kg cleans, add 50 premium on currency, smash to pieces with bruisher and to be homogenate,, filter with ultrasonic stirring 2 hours, repeat aforesaid operations 3 times, merge 4 times filtrate, the filtering residue response rate is 0.08%, the former powder yield 2.56kg of DSP.Yield: 2.56%.Total recovery: 2.64%.
The test of pesticide effectiveness:
1. the former powder hypoglycemic activity of DSP effect observation test: experiment material is the female Wistar rat, body weight 230-260g, and test method sees the special permission communique for details puts down into 5-19554, repeats no more here, and result of the test is as shown in table 3.The former powder of DSP is from example 10 sampling from table of table 1.
Table 3 is respectively organized blood glucose mean variation situation (mg/dl M ± SD)
* with before the administration compare P＜0.05 * * P＜0.01 △ and compare P＜0.05 △ △ P＜0.01 with positive control
| Group | Number of animals | Blood glucose value | |||||
| Before the administration | After the administration | ||||||
| 2 days | 4 days | 6 days | 8 days | 14 days | |||
| The former powder 20mg/kg of DSP | 11 | 347.0 (110.54) | 256.3△ (108.10) | 282.7△ (77.39) | 226.6 *△ (83.40) | 250.7 *△ (63.08) | 154.1 **△△ (30.48) |
| Glyburide 1.6mg/kg | 10 | 339.4 (119.65) | 280.4△ (112.61) | 300.1 (75.86) | 234.1△ (92.52) | 283.5 (133.21) | 243.2 (95.70) |
| Positive control | 11 | 323.6 (144.21) | 431.6 (182.93) | 410.0 (165.09) | 370.9 (137.26) | 416.8 (182.72) | 383.8 (199.98) |
Check through the t value, compare before each day after positive controls and the administration of glyburide group and the administration and find no statistical significance and difference (P＞0.05), and 5g/kg is organized in the 6th day and begins to have significance difference (P＜0.05), still has the difference (P＜0.01) of highly significant to compare with positive group after the drug withdrawal in 6 days: the 5g/kg group all promptly has remarkable reduction (P＜0.05) from the 2nd day beginning blood glucose; The glyburide group also had significant difference (P＜0.05) at 2 days and 5 days.
This shows that the former powder of DSP (fresh weight Crassula argentea Thunb extracting solution is dried to powder) acting duration is longer, drug withdrawal still can be kept blood sugar reducing function after 6 days.Thereby its blood sugar reducing function is better than glyburide.
The animal experiment of the former powder of DSP: method and material are the same, and result of the test is as shown in table 4
The former powder animal experiment of table 4 DSP
* be the sampling among the embodiment 2 of thawing of quick-freezing speed; * takes a sample among the ultrasonic disruption cell wall embodiment
| Group | Number of animals | Sex | Blood sugar content mg% | Blood glucose rate of descent (Δ X) (%) | The P value | ||
| Before the administration | After the administration | -ΔX±SD | |||||
| The former powder 20mg/kg of DSP * | 11 | ♀ | 198.41 | 146.52 | 51.89±40.99 | 51.89 | ＜0.01 |
| The former powder 20mg/kg of DSP ** | 11 | ♀ | 192.35 | 149.45 | 42.9±39.3 | 42.9 | ＜0.01 |
| Matched group | 11 | ♀ | 180.67 | 179.98 | 3.69±5.94 | 3.69 | —— |
3, the effective ingredient of new, old production technology and function ratio see Table 5.
Table 5
| Project construction technology | Drug effect (P value) | Total protein content % | Total sugar content % | Water solublity | Average yield |
| Old technology | ＜0.01 | 3-4.5 | ＜1 | Be partly dissolved | 1.58 |
| New technology | ＜0.01 | 30-60 | 4-6 | Molten entirely | 2.48 |
4, the mensuration of the albumen in the former powder of DSP, sugar, trace element etc.:
It is as shown in table 6 to press Chinese Pharmacopoeia method measurement result
1. table 6 albumen, sugar, water content test result
| The mensuration project | Content range | Peak | Minimum | Primary standard substance | Test instrunment |
| The total sugar amount | 4-6.0％ | 6％ | 4％ | D-mannose glutamer 200 | Ultraviolet-visible spectrophotometer-340 type, the differential thermal thermogravimetric analyzer |
| Protein content | 30-60％ | 60％ | 30％ | ||
| Water content | 5-8％ | 8％ | 5％ |
2. trace elements determination: measure with ICP (electron spectrum), Britain, test result is as shown in table 7.
Table 7
| Element term | Mg | Al | Fe | P | Cu | Zn | Ca | Pb | Cd | Cr | K + | Cl - |
| Content (mg/kg) | 20.3 | 0.2 | 0.48 | 10.3 | 0.03 | 0.17 | 62.2 | ＜1 | ＜1 | ＜1 | 55.0 | 68.0 |
| Percentage composition (%) | 2.03 | 0.02 | 0.048 | 1.03 | 0.003 | 0.017 | 6.22 | ＜0.1 | ＜0.1 | ＜0.1 | 5.5 | 6.8 |
5, the comparison of former powder of DSP and nutritive component of spirulina: as shown in table 8
Table 8
| Project content (%) title | Protein content % | Total sugar amount % | Trace element % |
| The former powder of DSP | 30-60 | 4-6 | ＞9 |
| Spirulina (dry weight) | 40-70 | 12-18 | 5-8 |
From table 3 to table 8 as seen, the drug effect of the former powder of DSP has the wide development prospect, and from the angle of nourishing healthy, albumen, sugar are all near spirulina, and trace element exceeds spirulina, and is very big as natural nutrition health food, health product, antidiabetic drug potentiality to be exploited.
Claims (2)
1, the extracting method of the former powder of Crassula argentea Thunb, comprise the stem and the leaf of bright Crassula argentea Thunb are cleaned, blend, the medicinal alcohol that adds the 49-5% of 0.5 times of amount soaked 4 hours, high-speed stirring mixes homogenate, filter, behind the filtrate recovered alcohol, be evaporated to and be thick and in 40-50 ℃ of infrared baking box, dry, pulverize and obtain the former powder of Crassula argentea Thunb.
2, will filter out the Lu slag according to the process of claim 1 wherein, be soaked in the water, after 24 hours, be heated to 50 ℃, be cooled to room temperature, filter pressing, filtering residue is pressed aforesaid operations and is repeated 4 times, merges 5 times filtrate.50-70 ℃ is evaporated to slightly thickly, puts into evaporating pan, is placed in the far ultraviolet baking box again, does 40-50 ℃ of baking, pulverizes, and crosses 60 mesh sieves, elaboration.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN95117499A CN1059568C (en) | 1995-11-24 | 1995-11-24 | Tech. for extracting nutrient and substance of lowering blood sugar from crassula portulacea a cactus plant |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN95117499A CN1059568C (en) | 1995-11-24 | 1995-11-24 | Tech. for extracting nutrient and substance of lowering blood sugar from crassula portulacea a cactus plant |
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| Publication Number | Publication Date |
|---|---|
| CN1151303A CN1151303A (en) | 1997-06-11 |
| CN1059568C true CN1059568C (en) | 2000-12-20 |
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| CN95117499A Expired - Lifetime CN1059568C (en) | 1995-11-24 | 1995-11-24 | Tech. for extracting nutrient and substance of lowering blood sugar from crassula portulacea a cactus plant |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03106819A (en) * | 1989-09-19 | 1991-05-07 | Kei Cho | Hypoglycemic drug |
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- 1995-11-24 CN CN95117499A patent/CN1059568C/en not_active Expired - Lifetime
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03106819A (en) * | 1989-09-19 | 1991-05-07 | Kei Cho | Hypoglycemic drug |
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| CN1151303A (en) | 1997-06-11 |
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Address after: No. 48, building A, building 1, Beijing science and Technology Exhibition Center, No. 3 West Third Ring Road, Beijing, Haidian District, 9C Patentee after: Junyang-Aima Science-Technology Co., Ltd., Beijing Address before: Room 108, building 404, Ze Ze garden, Peking University, Beijing Patentee before: Ding Jian |
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