CN105929152A - Intelligent detector for reducing procalcitonin - Google Patents
Intelligent detector for reducing procalcitonin Download PDFInfo
- Publication number
- CN105929152A CN105929152A CN201610561545.9A CN201610561545A CN105929152A CN 105929152 A CN105929152 A CN 105929152A CN 201610561545 A CN201610561545 A CN 201610561545A CN 105929152 A CN105929152 A CN 105929152A
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- Prior art keywords
- procalcitonin
- plate
- serum
- detection
- groove
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- 108010048233 Procalcitonin Proteins 0.000 title claims abstract description 36
- CWCXERYKLSEGEZ-KDKHKZEGSA-N procalcitonin Chemical compound C([C@@H](C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@H](C(=O)N[C@@H](C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](C(C)C)C(=O)NCC(=O)N[C@@H](C)C(=O)N1[C@@H](CCC1)C(=O)NCC(O)=O)[C@@H](C)O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCSC)NC(=O)[C@H]1NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(N)=O)NC(=O)CNC(=O)[C@@H](N)CSSC1)[C@@H](C)O)[C@@H](C)O)[C@@H](C)O)C1=CC=CC=C1 CWCXERYKLSEGEZ-KDKHKZEGSA-N 0.000 title claims abstract description 36
- 210000002966 serum Anatomy 0.000 claims abstract description 36
- 238000001514 detection method Methods 0.000 claims abstract description 26
- 239000011521 glass Substances 0.000 claims abstract description 7
- 210000002421 cell wall Anatomy 0.000 claims description 4
- 210000004369 blood Anatomy 0.000 claims description 2
- 239000008280 blood Substances 0.000 claims description 2
- 238000006243 chemical reaction Methods 0.000 abstract description 16
- 238000010521 absorption reaction Methods 0.000 abstract 1
- 239000011248 coating agent Substances 0.000 abstract 1
- 238000000576 coating method Methods 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- 239000000020 Nitrocellulose Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 229920001220 nitrocellulos Polymers 0.000 description 2
- 102000055006 Calcitonin Human genes 0.000 description 1
- 108060001064 Calcitonin Proteins 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 241000446313 Lamella Species 0.000 description 1
- 241000408521 Lucida Species 0.000 description 1
- 208000030852 Parasitic disease Diseases 0.000 description 1
- 206010040047 Sepsis Diseases 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 241000276425 Xiphophorus maculatus Species 0.000 description 1
- 208000026935 allergic disease Diseases 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
- 230000001363 autoimmune Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- BBBFJLBPOGFECG-VJVYQDLKSA-N calcitonin Chemical compound N([C@H](C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC=1NC=NC=1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H]([C@@H](C)O)C(=O)N1[C@@H](CCC1)C(N)=O)C(C)C)C(=O)[C@@H]1CSSC[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)O)C(=O)N1 BBBFJLBPOGFECG-VJVYQDLKSA-N 0.000 description 1
- 229960004015 calcitonin Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000003054 hormonal effect Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 238000010030 laminating Methods 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses an intelligent detector for detecting procalcitonin. The intelligent detector comprises a glass slide and a reaction plate, the reaction plate is arranged on the glass slide, the reaction plate is positioned rightly on the glass slide, the lower bottom surface of the reaction plate is laminated to the upper surface of the glass slide, the reaction plate is provided with a serum storage groove and a group of reaction grooves, one end of the reaction plate is provided with a serum adding channel connected with the serum storage groove, the inlet of the serum adding channel is positioned on the end surface of one end of the reaction plate, the serum storage groove in the reaction plate is connected with every reaction groove through a channel, the outer side of every reaction groove in the reaction plate is provided with liquid absorption paper, the channels are inclined channels, the side of every channel close to the serum storage groove is higher than the side of the corresponding channel close to the corresponding reaction groove, and a procalcitonin antibody coating positioning groove is arranged in every reaction groove. The intelligent detector for detecting procalcitonin has the advantages of reasonable structure design, realization of respective detection of various concentrations of a procalcitonin antibody through one detector, and high detection efficiency.
Description
Technical field
The present invention relates to technical field of biological, especially relate to a kind of for Procalcitonin. inspection
The intelligent detecting instrument surveyed.
Background technology
Procalcitonin. is the calcitonin precursor substance without hormonal activity.When serious bacterial, fungus,
When parasitic infection and sepsis and MOFE, its level in blood raises.
When autoimmune, allergy and virus infect, Procalcitonin. will not raise.During detection, needs are different
It is detected by the Procalcitonin. antibody of concentration respectively, it is ensured that the accuracy of detection.Existing
Intelligent detecting instrument can only single be carried out, and the operating time is long, and cost is high, and detection efficiency is low.
Summary of the invention
Not enough for prior art, the technical problem to be solved be to provide a kind of for
The intelligent detecting instrument of Procalcitonin. detection, the purpose high to reach detection efficiency.
In order to solve above-mentioned technical problem, the technical solution adopted in the present invention is:
This be used for Procalcitonin. detection intelligent detecting instrument, including slide and Sptting plate, described instead
Answering plate to be located on slide, Sptting plate is positioned at the surface of slide, bottom surface and slide below Sptting plate
Upper surface fit, described Sptting plate is provided with serum holding tank and one group of reactive tank, reaction
Plate one end is provided with the serum being connected with serum holding tank and adds passage, and serum adds entering of passage
Mouth is positioned on the end face of Sptting plate one end, and in described Sptting plate, serum holding tank passes through passage with each
Individual reactive tank is connected, and on described Sptting plate, the outside at each reactive tank is equipped with bibulous paper,
Described passage is ramp way, passage by serum holding tank side higher than by reactive tank side, described instead
It is provided with Procalcitonin. antibody in answering groove and is coated locating slot.
Further, described Procalcitonin. antibody is coated locating slot is the annulus being located in reactive tank
Groove, the cell wall of annular groove is provided with one group of through hole.
Passage on described Sptting plate includes that main channel and subchannel, one end, main channel are deposited with serum
Storage tank is connected, and the main channel other end is provided with end, main channel bibulous paper, and each reactive tank is by propping up
Passage is connected with main channel.
The upper surface edge of described slide is provided with raised item, and the bottom surface edge of Sptting plate is provided with convex
Play the detent that bar is corresponding.
Also including that box body, described box body are arranged above opening, opening is provided with the clamp of protrusion to inner side,
Sptting plate and slide are located between the base plate of box body and clamp.
Described detent is dovetail groove.
Also including that cover plate, described clamp are provided with draw-in groove, deck side edge can be inserted in draw-in groove.
The base plate of described box body is provided with opening.
The upper surface edge of described Sptting plate is provided with positioning convex strip, and described clamp is provided with and positions
The rail groove that raised line is corresponding.
Described cover plate is L-square, and L-square includes that transverse slat and riser, transverse slat are opened with box body top
Mouth is corresponding, and riser is corresponding with the opening of box body side, and riser is provided with and adds logical with serum
The opening in road.
The present invention compared with prior art, has the advantage that this is for Procalcitonin. detection
Intelligent detecting instrument reasonable in design, can be used for the Procalcitonin. of multiple concentration with a detector
It is detected by antibody the most simultaneously, it is not necessary to carry out repeated detection, and detection efficiency is high, reduces
Testing cost.
Accompanying drawing explanation
Content expressed by width accompanying drawing each to this specification and the labelling in figure are made briefly below
Bright:
Fig. 1 is Sptting plate structural representation of the present invention.
Fig. 2 is the left view of Fig. 1.
Fig. 3 is slide structural representation of the present invention.
Fig. 4 is box body structure schematic diagram of the present invention.
Fig. 5 is covering plate structure schematic diagram of the present invention.
In figure:
1. Sptting plate, 2. positioning convex strip, 3. serum holding tank, 4. main channel, 5. end, main channel
Bibulous paper, 6. reactive tank, 7. Procalcitonin. antibody be coated locating slot, 8. subchannel bibulous paper,
9. subchannel, 10. serum add passage, 11. slides, 12. raised items, 13. projections, 14. box bodys,
15. clamps, 16. rail grooves, 17. draw-in grooves, 18. cover plates.
Detailed description of the invention
Below against accompanying drawing, by the description to embodiment, the detailed description of the invention to the present invention
It is described in further detail.
As shown in Figures 1 to 5, this is used for the intelligent detecting instrument of Procalcitonin. detection, including glass
Sheet 11, Sptting plate 1, box body 14 and cover plate 18, wherein, Sptting plate is located on slide,
Sptting plate 1 is positioned at the surface of slide, and slide is the support carrier of Sptting plate, Sptting plate and glass
The size dimension of sheet is the same, is rectangle platy structure.
Below Sptting plate 1, bottom surface fits with the upper surface of slide 11, the upper surface of slide 11
Edge is provided with raised item 12, and the bottom surface edge of Sptting plate is provided with determine corresponding with raised item 12
Position groove, detent is dovetail groove, it is ensured that slide 11 upper surface and Sptting plate 1 bottom surface
Laminating effect；Being provided with projection inside raised item one end of slide, detent one end is provided with convex
The corresponding groove of block, positions Sptting plate and slide.Sptting plate is rubber slab, rubber slab with
Slide flexible contact, fits effective.
Sptting plate 1 is provided with serum holding tank 3 and one group of reactive tank 6, and Sptting plate 1 one end sets
Having the serum being connected with serum holding tank to add passage 10, serum adds the entrance of passage 10
Being positioned on the end face of Sptting plate 1 one end, serum adds the entrance of passage and can be connected with syringe,
It is easy to serum add.
In Sptting plate 1, serum holding tank 3 is connected with each reactive tank 6 by passage, reaction
On plate 1, the outside at each reactive tank is equipped with bibulous paper, and passage is ramp way, and passage leans on
Serum holding tank side, higher than by reactive tank side, is provided with Procalcitonin. antibody and is coated calmly in reactive tank 6
Position groove 7.
Passage includes main channel 4 and subchannel 9, one end, main channel 4 and serum holding tank 3 phase
Even, main channel 4 other end is provided with end, main channel bibulous paper 5, and each reactive tank is logical by propping up
Road is connected with main channel.On Sptting plate, the outside at each reactive tank is equipped with subchannel bibulous paper
8。
The serum added in serum holding tank 3 can be drawn onto reactive tank 6 automatically by bibulous paper
In, the Procalcitonin. antibody concentration that each Procalcitonin. antibody is coated in locating slot 7 is different, bag
By for nitrocellulose filter, nitrocellulose filter is Procalcitonin. antibody stationary support, with one
Individual detector can be used for the Procalcitonin. antibody of multiple concentration and detects it respectively, it is not necessary to enter
Row repeated detection, detection efficiency is high, and testing cost is low.
It is the annular groove being located in reactive tank that Procalcitonin. antibody is coated locating slot 7, annular groove
In cell wall is provided with one group of through hole, i.e. cell wall, outside communicates.The both sides of main channel 4 are equipped with props up
Passage 9, the subchannel of both sides, main channel shifts to install, it is ensured that the reliability of detection.
Box body 14 is used for accommodating Sptting plate 1 and slide 11, is fixed both.Box body is
Rectangular box-like structure, above box body and one side be opening, box body 1 upper opening is to inner side
It is provided with the clamp 15 of protrusion, Sptting plate 1 and slide 11 and is located at base plate and the clamp of box body 14
Between 15.Sptting plate and slide can be inserted in box body by the opening of box body side after coordinating.
The upper surface edge of Sptting plate 1 is provided with positioning convex strip 2, and clamp 1 is provided with and convex
The rail groove 16 that bar 2 is corresponding, Sptting plate and slide insert in box body 14, pass through positioning convex strip
Ensureing both positions in box body, fixing-stable is reliable.
Clamp 15 is provided with draw-in groove 17, and cover plate 18 side can be inserted in draw-in groove 17.Cover plate 18
For L-square, L-square includes transverse slat and riser, and transverse slat is corresponding with the opening on box body top,
Riser is corresponding with the opening of box body side.Riser is provided with and the opening of serum addition passage,
It is easy to add serum.Cover plate 18 is the plastic plate of hollow, and cover plate 18 is squeezed elastically deformable,
It is reliable that cover plate inserts location in draw-in groove, and cover plate 18 is lamella lucida.
The base plate of box body 14 is provided with opening or box body 14 is transparent box body, it is ensured that inspection
Survey effect；As the base plate of box body is provided with opening, opening can be rectangular opening, by opening
The support to slide of the edge of mouth.
Above in conjunction with accompanying drawing, the present invention is exemplarily described, it is clear that the present invention implements
It is not subject to the restrictions described above, as long as have employed the design of the present invention and technical scheme carries out
The improvement of various unsubstantialities, or the most improved design by the present invention and technical scheme directly should
For other occasion, all within protection scope of the present invention.
Claims (10)
1. the intelligent detecting instrument for Procalcitonin. detection, it is characterised in that: include glass
Sheet and Sptting plate, described Sptting plate is located on slide, and Sptting plate is positioned at the surface of slide, instead
Answering the upper surface of bottom surface and slide below plate to fit, described Sptting plate is provided with serum holding tank
With one group of reactive tank, Sptting plate one end is provided with the serum being connected with serum holding tank and adds logical
Road, serum adds the entrance of passage and is positioned on the end face of Sptting plate one end, blood in described Sptting plate
Clear holding tank is connected with each reactive tank by passage, at each reactive tank on described Sptting plate
Outside be equipped with bibulous paper, described passage is ramp way, and passage is high by serum holding tank side
In by reactive tank side, it is provided with Procalcitonin. antibody in described reactive tank and is coated locating slot.
2. as claimed in claim 1 for the intelligent detecting instrument of Procalcitonin. detection, its feature
It is: it is the annular groove being located in reactive tank that described Procalcitonin. antibody is coated locating slot, annulus
The cell wall of groove is provided with one group of through hole.
3. as claimed in claim 1 for the intelligent detecting instrument of Procalcitonin. detection, its feature
It is: the passage on described Sptting plate includes main channel and subchannel, one end, main channel and serum
Holding tank is connected, and the main channel other end is provided with end, main channel bibulous paper, and each reactive tank passes through
Subchannel is connected with main channel.
4. as claimed in claim 1 for the intelligent detecting instrument of Procalcitonin. detection, its feature
Be: the upper surface edge of described slide is provided with raised item, the bottom surface edge of Sptting plate be provided with
The detent that raised item is corresponding.
5. as claimed in claim 1 for the intelligent detecting instrument of Procalcitonin. detection, its feature
It is: also including that box body, described box body are arranged above opening, opening is provided with the card of protrusion to inner side
Plate, Sptting plate and slide are located between the base plate of box body and clamp.
6. as claimed in claim 4 for the intelligent detecting instrument of Procalcitonin. detection, its feature
It is: described detent is dovetail groove.
7. as claimed in claim 5 for the intelligent detecting instrument of Procalcitonin. detection, its feature
It is: also including that cover plate, described clamp are provided with draw-in groove, deck side edge can be inserted in draw-in groove.
8. as claimed in claim 5 for the intelligent detecting instrument of Procalcitonin. detection, its feature
It is: the base plate of described box body is provided with opening.
9. as claimed in claim 5 for the intelligent detecting instrument of Procalcitonin. detection, its feature
Being: the upper surface edge of described Sptting plate is provided with positioning convex strip, described clamp is provided with fixed
The rail groove that position raised line is corresponding.
10. as claimed in claim 7 for the intelligent detecting instrument of Procalcitonin. detection, its feature
Being: described cover plate is L-square, L-square includes transverse slat and riser, transverse slat and box body top
Opening is corresponding, and riser is corresponding with the opening of box body side, and riser is provided with and adds with serum
The opening of passage.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610561545.9A CN105929152B (en) | 2016-07-15 | 2016-07-15 | A kind of intelligent detecting instrument for Procalcitonin detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610561545.9A CN105929152B (en) | 2016-07-15 | 2016-07-15 | A kind of intelligent detecting instrument for Procalcitonin detection |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105929152A true CN105929152A (en) | 2016-09-07 |
| CN105929152B CN105929152B (en) | 2018-06-26 |
Family
ID=56827574
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610561545.9A Active CN105929152B (en) | 2016-07-15 | 2016-07-15 | A kind of intelligent detecting instrument for Procalcitonin detection |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105929152B (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2347894Y (en) * | 1998-09-08 | 1999-11-10 | 叶凯伦 | Storage box |
| CN1403817A (en) * | 2002-10-22 | 2003-03-19 | 中国人民解放军南京军区联勤部军事医学研究所 | Immune cell microfluid array |
| CN2565022Y (en) * | 2002-08-23 | 2003-08-06 | 上海博昇微晶科技有限公司 | Microfluid chip with three-layer structure |
| WO2007121263A2 (en) * | 2006-04-14 | 2007-10-25 | Wako Pure Chemical Industries, Ltd | Structure for introducing a plurality of solutions, micro fluidic device having said structure and method for introducing solution |
| CN101382538A (en) * | 2007-09-04 | 2009-03-11 | 财团法人工业技术研究院 | Auto-shunting microfluid apparatus |
| CN203101401U (en) * | 2012-11-12 | 2013-07-31 | 南京诺尔曼生物技术有限公司 | Procalcitonin whole blood detecting device |
| WO2013153177A1 (en) * | 2012-04-12 | 2013-10-17 | B.R.A.H.M.S Gmbh | Prognosis of adverse events in patients with suspected chronic heart failure |
| CN104226385A (en) * | 2013-06-09 | 2014-12-24 | 北京百康芯生物科技有限公司 | A novel micro-fluidic chip and a using method thereof |
| CN204336778U (en) * | 2015-01-09 | 2015-05-20 | 王兴民 | New-type antiskid soapbox |
-
2016
- 2016-07-15 CN CN201610561545.9A patent/CN105929152B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2347894Y (en) * | 1998-09-08 | 1999-11-10 | 叶凯伦 | Storage box |
| CN2565022Y (en) * | 2002-08-23 | 2003-08-06 | 上海博昇微晶科技有限公司 | Microfluid chip with three-layer structure |
| CN1403817A (en) * | 2002-10-22 | 2003-03-19 | 中国人民解放军南京军区联勤部军事医学研究所 | Immune cell microfluid array |
| WO2007121263A2 (en) * | 2006-04-14 | 2007-10-25 | Wako Pure Chemical Industries, Ltd | Structure for introducing a plurality of solutions, micro fluidic device having said structure and method for introducing solution |
| CN101382538A (en) * | 2007-09-04 | 2009-03-11 | 财团法人工业技术研究院 | Auto-shunting microfluid apparatus |
| WO2013153177A1 (en) * | 2012-04-12 | 2013-10-17 | B.R.A.H.M.S Gmbh | Prognosis of adverse events in patients with suspected chronic heart failure |
| CN203101401U (en) * | 2012-11-12 | 2013-07-31 | 南京诺尔曼生物技术有限公司 | Procalcitonin whole blood detecting device |
| CN104226385A (en) * | 2013-06-09 | 2014-12-24 | 北京百康芯生物科技有限公司 | A novel micro-fluidic chip and a using method thereof |
| CN204336778U (en) * | 2015-01-09 | 2015-05-20 | 王兴民 | New-type antiskid soapbox |
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| Publication number | Publication date |
|---|---|
| CN105929152B (en) | 2018-06-26 |
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