CN105929051B - A kind of milk powder mysoinositol assay method - Google Patents
A kind of milk powder mysoinositol assay method Download PDFInfo
- Publication number
- CN105929051B CN105929051B CN201610247906.2A CN201610247906A CN105929051B CN 105929051 B CN105929051 B CN 105929051B CN 201610247906 A CN201610247906 A CN 201610247906A CN 105929051 B CN105929051 B CN 105929051B
- Authority
- CN
- China
- Prior art keywords
- solution
- milk powder
- mentioned
- added
- mysoinositol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N2030/022—Column chromatography characterised by the kind of separation mechanism
- G01N2030/025—Gas chromatography
Abstract
The invention discloses a kind of milk powder mysoinositol assay method, this method includes the following steps：Milk powder to be measured is fully dissolved with solution of trichloroacetic acid；It draws in above-mentioned milk power solution to beaker to be measured, dries, quickly to obtain drying sample；Silylating reagent is added in above-mentioned drying sample, ultrasonic dissolution carries out derivatization reaction, to obtain derivatization reaction product；N-hexane, concussion mixing are added in after above-mentioned derivatization reaction product cooling, stratification takes supernatant to centrifuge to being previously added in the centrifuge tube of a small amount of anhydrous sodium sulfate, take supernatant liquid filtering, upper machine testing solution is treated to obtain；Upper machine testing solution is treated with gas chromatograph for determination is above-mentioned.Milk powder mysoinositol assay method provided by the invention, easy to operate, quick, cost is relatively low, precision, stability and accuracy are high than existing methods.
Description
Technical field
The present invention relates to Food Chemistry detection field, specifically, the present invention relates to inositols to measure field, more specifically, this
Invention is related to a kind of milk powder mysoinositol assay method.
Background technology
Inositol, scientific name inositol are widely present in various natural animals, plant and microorganism cultures, because initially by
It is extracted in musculature, therefore obtains the name of inositol.Inositol belongs to B family vitamin, plays the role of similar to B1 and biotin, is people
Class maintains the indispensable low molecule organic matter of normal physiological function with animal, can promote the metabolism of liver and fat, in infant
It is also indispensable nutrition fortifier in milk powder.
The detection method of inositol is mainly at present《The survey of national food safety standard-infant food and dairy products mysoinositol
It is fixed》(GB5413.25-2010) the second method in, pre-treating method are, with 95% ethyl alcohol constant volume, will to be revolved after milk powder water dissolution
Derive upper machine testing with derivating agent after being evaporated.Because derivatization process there cannot be water, otherwise derive that effect is bad leads to testing result
Inaccuracy, so revolving thoroughly must cause the revolving time longer, bulk processing sample is cumbersome, and sample collimation is poor.
In addition,《The measure of national food safety standard-infant food and dairy products mysoinositol》(GB5413.25-2010) in
In two methods, with 95% ethyl alcohol constant volume after milk powder water dissolution, protein in milk powder and fatty second is dissolved in together with inositol
In alcohol, cause accuracy, precision and stability that inositol detects low.
Thus, still have much room for improvement to the method for measuring of milk powder mysoinositol.
Invention content
The present invention is directed to solve at least some of the technical problems in related technologies.For this purpose, the present invention
One purpose be to propose it is a kind of can quickly, stablize, the method for Accurate Determining milk powder mysoinositol.
The present invention is the following discovery based on inventor and completes：First, when rotating in milk powder mysoinositol assay method
Between it is long, one side detection cycle is long, on the other hand cause measure stability it is low with precision.Second, the egg in milk powder
White matter, fat and impurity are dissolved in ethyl alcohol, and accuracy, precision and stability that inositol measures can be caused low.
Thus, according to an aspect of the present invention, the present invention provides a kind of assay methods of milk powder mysoinositol.According to this
The embodiment of invention, this method include the following steps：Milk powder to be measured is fully dissolved with water or solution of trichloroacetic acid；Draw above-mentioned treat
It surveys in milk power solution to beaker, drying, quickly to obtain drying sample；Silylating reagent is added in above-mentioned drying sample, is surpassed
Sound dissolves, and derivatization reaction is carried out, to obtain derivatization reaction product；N-hexane is added in after above-mentioned derivatization reaction product cooling, is shaken
Mixing is swung, stratification takes supernatant to centrifuge to being previously added in the centrifuge tube of a small amount of anhydrous sodium sulfate, take supernatant liquid filtering,
Upper machine testing solution is treated to obtain；Upper machine testing solution is treated with gas chromatograph for determination is above-mentioned.
Inventor has surprisingly found that, using the milk powder mysoinositol assay method of the present invention, milk to be measured is dried using drying box
Powder solution, not only easy to operate, detection cycle shortens, and improves the precision and stability of inositol measure.
The assay method of milk powder mysoinositol according to embodiments of the present invention can also further increase step：Use trichloroacetic acid
Solution fully dissolves milk powder to be measured, acetic acid lead solution is then added in, finally with solution of trichloroacetic acid constant volume.
During milk powder is dissolved in ethapon solution, inositol, protein, fat and other impurity are molten together
Solution exists jointly always in ethapon solution in subsequent step, and inositol measure is interfered.Made using lead acetate
It for precipitating reagent, after protein, adipopexis, treats that machine testing solution is more clarified, effectively increases the stabilization of inositol measure
Property and accuracy.
In addition, the assay method of milk powder mysoinositol according to embodiments of the present invention can also further increase step：
The content of milk powder mysoinositol is calculated according to the result of gas chromatograph for determination.
Inositol standard solution is prepared, prepares inositol standard curve.
According to an embodiment of the invention, a concentration of 0.010mg/ml of inositol standard solution.
According to an embodiment of the invention, after a concentration of 2% trichloroacetic acid dissolving of milk powder to be measured, 12% lead acetate is added in
Solution precipitating proteins and fat, and with a concentration of 2% trichloroacetic acid constant volume.
According to an embodiment of the invention, 1g milk powder to be measured is weighed, after the trichloroacetic acid dissolving of 10ml a concentration of 2%, is added
Enter the acetic acid lead solution precipitating proteins and fat of 2ml a concentration of 12%, and be settled to a concentration of 2% trichloroacetic acid
50ml。
According to an embodiment of the invention, in the drying box that milk power solution to be measured is 102 DEG C ± 1 DEG C in temperature, baking 45min~
60min, until it is completely dried.
According to an embodiment of the invention, drying sample adds in silylating reagent, after ultrasonic dissolution, in 80 DEG C of ± 2 DEG C of water-baths
During which it is primary to take out concussion every 20min by derived reaction 75min.
According to an embodiment of the invention, 5ml n-hexanes are added in after derivatization reaction product is cooled to room temperature, concussion mixing is quiet
Layering is put, taking supernatant 3ml, 4000r/min centrifugation 5min take supernatant to being previously added in the centrifuge tube of a small amount of anhydrous sodium sulfate
Liquid is filtered with 0.22 μm of organic filter, and upper machine testing solution is treated to obtain.Derivatization reaction product, through n-hexane concussion mixing again
It after stratification, first centrifuges, then is filtered with 0.22 μm of organic filter, fully remove impurity, ensure that measure to the full extent
Precision, stability and accuracy.
It should be noted that according to the inventors knowledge, milk power solution to be measured is dried using drying box；And utilize trichloroacetic acid
Solution fully dissolves milk powder to be measured, acetic acid lead solution is then added in as precipitating reagent, by protein, fatty and other impurity removals
Sample-pretreating method, have not yet to see relevant report.The milk powder mysoinositol assay method that inventor provides, uses furnace drying method
It is easy to operate, quick, cost is relatively low by moisture removal in sample, improve the precision and stability of measure；On the other hand,
Using precipitating reagent by the protein in sample solution, fatty and other impurity removals make to treat that machine testing solution is more clarified, have
Effect improves stability and the accuracy of inositol measure.
Description of the drawings
The above-mentioned and/or additional aspect and advantage of the present invention is from combining in description of the accompanying drawings below to embodiment by change
It obtains significantly and is readily appreciated that, wherein：
Fig. 1 shows one embodiment of the present of invention mysoinositol canonical plotting.
Specific embodiment
Specific embodiment is that the present invention is described in detail below, and the embodiments described below is exemplary, only
For explaining the present invention, and it is not considered as limiting the invention.
Below except as otherwise explaining, the reagent do not explained especially and instrument involved in following embodiment all may be from routine
Commercial product.
The reagent and solution used in embodiment
Ultra-pure water
Solution of trichloroacetic acid (2%)：20g trichloroacetic acids accurately are weighed in 1L volumetric flasks, with water dissolution and are settled to quarter
It spends, it is spare after mixing.
12% acetic acid lead solution (12%)：120g lead acetates accurately are weighed in 1L volumetric flasks, with water dissolution and are settled to
Scale, it is spare after mixing.
N-hexane：Chromatographically pure
Ethyl alcohol (95%)
Ethyl alcohol (70%)
Trim,ethylchlorosilane
Hexamethyldisilazane
N,N-dimethylformamide
Silylating reagent：Volume aspirated ratio is 1 respectively:2:8 trim,ethylchlorosilane, hexamethyldisilazane, N, N- bis-
Methylformamide, ultrasonic mixing, prepared before use.
Inositol standard items：Purity >=99%, producer：SIGMA, article No.：090M142V
The instrument and equipment used in embodiment
Balance：Sensibility reciprocal is 0.1mg, producer：METTLER TOLEDO, model：XP205
Gas chromatograph：Band fid detector, producer：Varian, model：GC-450
Centrifuge：Producer：Heal Force, model：Neofuge 23R
Supersonic wave cleaning machine：Producer：Flag is beautiful, model：QC10260
Thermostatic drying chamber：Producer：Shanghai Fuma Experiment Equipment Co., Ltd., model：DGX-9243B-1
Thermostat water bath：Producer：Shanghai Fuma Experiment Equipment Co., Ltd., model：HHW-21CU-600B
30ml screw thread test tubes
It is prepared by 1 inositol standard curve of embodiment
1. prepare inositol standard solution
Inositol standard solution (0.010mg/ml)：The accurate 100mg (being accurate to 0.1mg) that weighs dries 2h's by 105 DEG C
Inositol standard items are complete with 25ml water dissolutions in 100ml volumetric flasks, and graduation mark is settled to 95% ethyl alcohol.Take this solution of 1ml
With in 100ml volumetric flasks, graduation mark, mixing are settled to 70% ethyl alcohol.
2. prepare inositol standard curve
1) 0.0ml is drawn respectively, and 0.2ml, 0.4ml, 0.6ml, 0.8ml, 1.0ml inositols standard solution is in 25ml beakers
In, it is put into constant temperature, 102 DEG C ± 1 DEG C, dries 45min~60min to being completely dried, obtain dry inositol；
2) 10ml silylating reagents are separately added into above-mentioned dry inositol, ultrasonic dissolution 5min is simultaneously shifted in the examination of 30ml screw threads
Guan Zhong is positioned over reaction 75min derived from 80 DEG C of ± 2 DEG C of water-baths, during which takes out concussion once every 20min, obtains derivative anti-
Answer product；
3) above-mentioned derivatization reaction product, which is cooled to room temperature, adds in 5ml n-hexanes, and concussion mixing, stratification takes supernatant
For 3ml to being previously added in the centrifuge tube of a small amount of anhydrous sodium sulfate, 4000r/min centrifugation 5min draw 1ml sample solutions with 0.22
μm organic filter filtering, filtered fluid is collected in 2mL sample injection bottles, to be measured.
4) liquid chromatograph detects, chromatographic column：Agilient Technology DB-5 chromatographic columns (60m × 0.25mm,
0.25μm)；Injector temperature：260℃；Detector temperature：300℃；Split ratio：10:1；Flow velocity:2.0mL/min；Sample size：
1ul；Temperature programming is shown in Table 1：
1 temperature programming of table
Using peak area as ordinate, a concentration of abscissa draws standard curve (coefficient R 2 >=0.99), referring to Fig. 1.
Embodiment 2
A kind of assay method of milk powder mysoinositol, includes the following steps：
1) 1g milk powder sample (being accurate to 0.1mg) is weighed in 50mL volumetric flasks, first adds in 20mL water dissolutions, 10 points of ultrasound
Zhong Houyong water constant volume mixings, obtain milk power solution to be measured；
2) the above-mentioned milk power solutions to be measured of 1ml are taken in 25ml beakers, with standard working solution together in thermostatic drying chamber,
102 DEG C ± 1 DEG C, 45min~60min is dried to being completely dried, obtains drying sample；
3) 10ml silylating reagents are added in above-mentioned drying sample, ultrasonic dissolution 5min is simultaneously shifted in 30ml screw thread test tubes
In, reaction 75min derived from 80 DEG C of ± 2 DEG C of water-baths is positioned over, during which concussion is taken out once every 20min, obtains derivatization reaction
Product；
4) above-mentioned derivatization reaction product, which is cooled to room temperature, adds in 5ml n-hexanes, and concussion mixing, stratification takes supernatant
For 3ml to being previously added in the centrifuge tube of a small amount of anhydrous sodium sulfate, 4000r/min centrifugation 5min draw 1ml sample solutions with 0.22
μm organic filter filtering, filtered fluid is collected in 2mL sample injection bottles, to be measured.
5) liquid chromatograph detects, chromatographic column：Agilient Technology DB-5 chromatographic columns (60m × 0.25mm,
0.25μm)；Injector temperature：260℃；Detector temperature：300℃；Split ratio：10:1；Flow velocity：2.0mL/min；Sample size：
1ul；Temperature programming is shown in Table 1.
Do not add precipitating reagent (12% acetic acid lead solution) in embodiment 2 in the pretreatment process of milk powder sample.
Embodiment 3
A kind of assay method of milk powder mysoinositol, includes the following steps：
1) 1g milk powder sample (being accurate to 0.1mg) is weighed in 50mL volumetric flasks, is dissolved with 2% solution of trichloroacetic acid, is surpassed
After sonication 10min, 2% acetic acid lead solutions of 2ml are added in, are finally settled to graduation mark mixing with 2% solution of trichloroacetic acid；
2) the above-mentioned milk power solutions to be measured of 10ml are taken in 50ml centrifuge tubes, 10000r/min centrifugations 10min, accurate absorption
1.00ml supernatants are in 25ml beakers, with standard working solution together in thermostatic drying chamber, 102 DEG C ± 1 DEG C, dry 45min~
60min obtains drying sample to being completely dried；
3) 10ml silylating reagents are added in above-mentioned drying sample, ultrasonic dissolution 5min is simultaneously shifted in 30ml screw thread test tubes
In, reaction 75min derived from 80 DEG C of ± 2 DEG C of water-baths is positioned over, during which concussion is taken out once every 20min, obtains derivatization reaction
Product；
4) above-mentioned derivatization reaction product, which is cooled to room temperature, adds in 5ml n-hexanes, and concussion mixing, stratification takes supernatant
For 3ml to being previously added in the centrifuge tube of a small amount of anhydrous sodium sulfate, 4000r/min centrifugation 5min draw 1ml sample solutions with 0.22
μm organic filter filtering, filtered fluid is collected in 2mL sample injection bottles, to be measured.
5) liquid chromatograph detects, chromatographic column：Agilient Technology DB-5 chromatographic columns (60m × 0.25mm,
0.25μm)；Injector temperature：260℃；Detector temperature：300℃；Split ratio：10:1；Flow velocity：2.0mL/min；Sample size：
1ul；Temperature programming is shown in Table 1.
Precipitating reagent (12% acetic acid lead solution) is added in embodiment 3 in the pretreatment process of milk powder sample.
Embodiment 2 is compared with 3 testing result of embodiment：
Do not add precipitating reagent (12% acetic acid lead solution) in embodiment 2 in the pretreatment process of milk powder sample.
Precipitating reagent (12% acetic acid lead solution) is added in embodiment 3 in the pretreatment process of milk powder sample.
It has been found by contrast that 2 milk power solution to be measured of embodiment is more muddy, it is difficult to it is accurate to draw, and in derivatization reaction mistake
Impurity is relatively more in journey, and accuracy and balance are poor, and comparative analysis during Sample pretreatment the result shows that add in precipitation
Agent, accuracy, precision and the stability of testing result all increase.Specific experiment data are shown in Table 2.
2 embodiment 2 of table is compared with 3 testing result of embodiment
| Serial number | Embodiment 2 | Embodiment 3 |
| 1 | 98.5 | 102 |
| 2 | 95.3 | 97.2 |
| 3 | 101 | 98.7 |
| 4 | 91.7 | 97.9 |
| 5 | 93.8 | 99.5 |
| 6 | 92.6 | 98.2 |
| Average value | 95.5 | 98.9 |
| RSD% | 3.78 | 1.70 |
Comparative example
With《The measure of national food safety standard-infant food and dairy products mysoinositol》(GB5413.25-2010) in
Second method measures milk powder mysoinositol content；
Simultaneously milk powder mysoinositol content is measured with 3 the method for embodiment.
It detects in powdered milk sample and is quantitatively added without inositol, inositol.
《The measure of national food safety standard-infant food and dairy products mysoinositol》(GB5413.25-2010) second in
The measurement result of method, as shown in table 3.
Preci-sion and accuracy (n=6) experiment of 3 present invention of table
3 the method measurement result of embodiment, as shown in table 4.
Preci-sion and accuracy (n=6) experiment of 4 national standard method of table
Conclusion：The preci-sion and accuracy for proving the method for the invention by more than contrast test is all higher than national standard side
Method.
In the description of this specification, reference term " one embodiment ", " example ", " is specifically shown " some embodiments "
The description of example " or " some examples " etc. means that combining the embodiment or specific features, material or the feature of example description includes
In at least one embodiment of the present invention or example.In the present specification, schematic expression of the above terms not necessarily refer to
Be identical embodiment or example.Moreover, particular features, structures, materials, or characteristics described can be at any one
Or it is combined in an appropriate manner in multiple embodiments or example.
Although an embodiment of the present invention has been shown and described, it will be understood by those skilled in the art that：Not
In the case of being detached from the principle of the present invention and objective a variety of change, modification, replacement and modification can be carried out to these embodiments, this
The range of invention is limited by claim and its equivalent.
Claims (7)
1. a kind of milk powder mysoinositol assay method, which is characterized in that including：
1) 1g milk powder to be measured is weighed, after the trichloroacetic acid dissolving of 10ml a concentration of 2%, adds in the acetic acid of 2ml a concentration of 12%
Lead solution precipitating proteins and fat, and it is settled to 50ml with a concentration of 2% trichloroacetic acid；
2) it draws in above-mentioned milk power solution to beaker to be measured, drying, quickly to obtain drying sample；
3) silylating reagent is added in above-mentioned drying sample, ultrasonic dissolution carries out derivatization reaction, to obtain derivatization reaction production
Object；
4) n-hexane, concussion mixing are added in after above-mentioned derivatization reaction product cooling, stratification takes supernatant few to being previously added
In the centrifuge tube for measuring anhydrous sodium sulfate, centrifugation takes supernatant liquid filtering, upper machine testing solution is treated to obtain；
5) upper machine testing solution is treated with gas chromatograph for determination is above-mentioned.
2. according to the method described in claim 1, it is characterized in that, further comprise step, according to gas chromatograph for determination
As a result the content of milk powder mysoinositol is calculated.
3. according to the method described in claim 2, it is characterized in that, further comprising step, prepared by preparation inositol standard solution
Inositol standard curve.
4. according to the method described in claim 1-3 any one, which is characterized in that the milk power solution to be measured is in temperature
In 102 DEG C ± 1 DEG C of drying box, 45min~60min is dried, until it is completely dried.
5. according to the method described in claims 1 to 3 any one, which is characterized in that the drying sample adds in silanization examination
After ultrasonic dissolution, in reaction 75min derived from 80 DEG C of ± 2 DEG C of water-baths, it is primary during which to take out concussion every 20min for agent.
6. according to the method described in claims 1 to 3 any one, which is characterized in that the derivatization reaction product is cooled to room
5ml n-hexanes, concussion mixing are added in after temperature, stratification takes supernatant 3ml to the centrifugation for being previously added a small amount of anhydrous sodium sulfate
Guan Zhong, 4000r/min centrifuge 5min, and supernatant is taken to be filtered with 0.22 μm of organic filter, upper machine testing solution is treated to obtain.
7. the according to the method described in claim 3, it is characterized in that, a concentration of 0.010mg/ml of the inositol standard solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610247906.2A CN105929051B (en) | 2016-04-20 | 2016-04-20 | A kind of milk powder mysoinositol assay method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610247906.2A CN105929051B (en) | 2016-04-20 | 2016-04-20 | A kind of milk powder mysoinositol assay method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105929051A CN105929051A (en) | 2016-09-07 |
| CN105929051B true CN105929051B (en) | 2018-06-29 |
Family
ID=56839420
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610247906.2A Active CN105929051B (en) | 2016-04-20 | 2016-04-20 | A kind of milk powder mysoinositol assay method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105929051B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115901909A (en) * | 2021-08-12 | 2023-04-04 | 西南医科大学附属医院 | Method for quantitatively detecting myoinositol in blood |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS58109849A (en) * | 1981-12-24 | 1983-06-30 | Asahi Chem Ind Co Ltd | Novel analysis for separation of inositol |
| CN104374845A (en) * | 2014-11-19 | 2015-02-25 | 广东省农业科学院蔬菜研究所 | Method for simultaneously detecting pumpkin fruit inositol, monosaccharide and disaccharide substances |
| CN104897821A (en) * | 2015-06-03 | 2015-09-09 | 福建中烟工业有限责任公司 | Method and kit for extracting inositol in sample, and application of kit |
-
2016
- 2016-04-20 CN CN201610247906.2A patent/CN105929051B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS58109849A (en) * | 1981-12-24 | 1983-06-30 | Asahi Chem Ind Co Ltd | Novel analysis for separation of inositol |
| CN104374845A (en) * | 2014-11-19 | 2015-02-25 | 广东省农业科学院蔬菜研究所 | Method for simultaneously detecting pumpkin fruit inositol, monosaccharide and disaccharide substances |
| CN104897821A (en) * | 2015-06-03 | 2015-09-09 | 福建中烟工业有限责任公司 | Method and kit for extracting inositol in sample, and application of kit |
Non-Patent Citations (5)
| Title |
|---|
| Determination of myo-inositol in infant formulae and milk powders using capillary gaschromatography with flame ionisation detection;Woollard DC等;《INTERNATIONAL DAIRY JOURNAL》;20140831;第37卷(第2期);74-81 * |
| 固相萃取-气相色谱法测定婴幼儿配方奶粉中肌醇含量;曾静等;《检验检疫学刊》;20141231;第24卷(第2期);40-43，34 * |
| 奶粉中肌醇的气相色谱-质谱法测定;罗海英等;《食品科学》;20101231;第31卷(第2期);117-119 * |
| 检测牛奶及奶粉中肌醇的气相色谱法研究;蔡秋杏等;《钦州学院学报》;20111231;第26卷(第6期);44-46 * |
| 黄芪多糖中脱蛋白方法的研究;高美风等;《中华中医药学刊》;20080331;第26卷(第3期);614-615 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105929051A (en) | 2016-09-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104892486B (en) | The crystal formation B of Apremilast+And preparation method thereof | |
| CN102212514B (en) | Blood coagulant for blood collection container | |
| CN102319210A (en) | A kind of long-acting veterinary cefquinome sulfate injection and preparation method thereof | |
| CN102018669B (en) | Long-acting ceftiofur hydrochloride injection and preparation method thereof | |
| CN106324183A (en) | Soil humus extraction and purification method | |
| CN105929051B (en) | A kind of milk powder mysoinositol assay method | |
| CN105116063B (en) | The multi-joint detection method of cephalosporins medicine residual in a kind of dairy product | |
| CN104181313B (en) | Factor IX quality-control product preparation method | |
| CN109142597A (en) | The pre-treating method of Tilmicosin in a kind of detection Swine plasma | |
| CN109633028A (en) | The detection method of spice additive in a kind of special medicine purposes formula food | |
| CN105987961A (en) | Method for detecting levetiracetam in breast milk | |
| CN110715986A (en) | Method for determining glucosamine hydrochloride in health food | |
| CN107831259A (en) | The assay method of free amino acid in a kind of spleen aminopeptide | |
| CN102539592A (en) | Method for detecting content of VLCFAs (very long chain fatty acids) in body fluid | |
| CN112505223B (en) | Method for simultaneously detecting content of toxoflavin and content of mirostrobin in food | |
| CN101762654B (en) | Determination method for content of monoglyceride in liquid milk | |
| CN104034823B (en) | The detection method of indoles alkaloid composition in a kind of dried venom of toads medicinal material | |
| CN104483411B (en) | A kind of capsule of weeping forsythia and the detection method containing capsule of weeping forsythia product | |
| CN107621509A (en) | The method for quantitatively determining of the ester of 1,3 two oleic acid, 2 palmitic acid three in baby formula milk powder | |
| CN105987962A (en) | Method for detecting lamotrigine in breast milk | |
| CN106918487B (en) | Preparation method of dibutyl phthalate standard sample | |
| CN108226308A (en) | The method of dehydroactic acid in determination sample | |
| CN110075179A (en) | A kind of spot lip resupinate woodbetony leaf or root granule, preparation method and its detection method | |
| CN110687224A (en) | Method for measuring triptolide A in tripterygium wilfordii medicinal material and tripterygium wilfordii multi-glycoside tablet prepared from tripterygium wilfordii medicinal material | |
| CN103926368A (en) | Method for extracting biotin from corn steep liquor and thin layer chromatography (TLC) scanning detection method of biotin |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |