CN105928898A - Rapidly established terahertz attenuation total reflection system based on Dove prism - Google Patents

Rapidly established terahertz attenuation total reflection system based on Dove prism Download PDF

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Publication number
CN105928898A
CN105928898A CN201610234800.9A CN201610234800A CN105928898A CN 105928898 A CN105928898 A CN 105928898A CN 201610234800 A CN201610234800 A CN 201610234800A CN 105928898 A CN105928898 A CN 105928898A
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terahertz
prism
dove prism
system based
attenuated total
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Inventor
府伟灵
赵祥
黄庆
罗阳
杨翔
张阳
刘跃平
徐含青
刘羽
杨柯
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First Affiliated Hospital of TMMU
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First Affiliated Hospital of TMMU
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G01N21/35Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
    • G01N21/3581Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light using far infrared light; using Terahertz radiation
    • G01N21/3586Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light using far infrared light; using Terahertz radiation by Terahertz time domain spectroscopy [THz-TDS]

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  • Physics & Mathematics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Health & Medical Sciences (AREA)
  • Toxicology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

The invention discloses a rapidly established terahertz attenuation total reflection system based on Dove prism, and belongs to the technical field of optical tests. The system comprises the following components arranged in sequence: a femtosecond laser, a beam splitter, a terahertz generator, a prism, a terahertz receiver and a signal processing system. The prism is a dove prism, whose axial section is in an isosceles trapezoid shape. The dove prism is made of a silicon material with high resistance and a high refractive index, and a reflector is arranged between the beam splitter and the terahertz receiver. By adopting a dove prism with an isosceles trapezoidal axial section, an attenuation total reflection system for detecting the terahertz spectrum of a sample can be established rapidly by parallelly arranging the prism in the optical path, the prism is not needed to be braced by a prism support, the overall optical path is not needed to be adjusted, the establishing time and material consumption are greatly reduced, at the same time, the prism surface can completely cover evanescent waves, the using amount of tested sample is obviously reduced, and thus the using cost is reduced.

Description

A kind of Terahertz attenuated total reflectance system based on Dove prism fast construction
Technical field
The invention belongs to technical field of optical test, be specifically related to the decay of a kind of Terahertz based on Dove prism fast construction and be all-trans Penetrate system.
Background technology
Terahertz (Terahertz, THz) ripple refer to wave band between microwave and infrared electromagnetic radiation, frequency is at 0.1THz-10THz Between (1THz=1012Hz), wavelength is between 30 μm-3000 μm.Comparing microwave, Terahertz wavelength and pulse are shorter, tool There are higher time and spatial resolution.Its photon energy less (at 1THz, photon energy is about 4meV) simultaneously, will not be to thing Matter produces destruction, compares x-ray safer.The low frequencies such as particularly skeletal vibration and the configuration of biomolecule bends, rotation Vibration mode is in THz wave energy range, and tera-hertz spectra comprises the abundant physics of biological substance and chemical information, by extensively General being applied to includes the field of biology such as nucleic acid, protein, antibacterial, cell and cancerous tissue imaging.
When the spectral information of biological specimen measured by tradition terahertz light spectrometer with transmission mode, on the one hand by sample moisture content relatively Gao Ying Ring, it usually needs tissue, antibacterial equal samples are weakened water to transmission terahertz signal by the mode such as vacuum freeze-drying or paraffin embedding Decay, the biomacromolecule including aminoacid is generally mixed with polyethylene powders (terahertz wave band is nearly transparent) Tabletting carries out transmission measurement.Above method cannot study the physical feature under biological specimen solution environmental in vivo, to THz wave Technology is applied to biological study and brings bigger restriction.On the other hand, Terahertz wavelength (being 300 μm at 1THz) is much larger than antibacterial (1-2 μm), the diameter of cell (about 10 μm), tradition Terahertz detection technique is limited by diffraction limit, there is detection chi Degree loses coupling, it is difficult to accurately respond the shortcomings such as intercellular difference signal.
THz wave attenuated total reflectance refers to that incident THz wave can produce in interface when the internal experiences total internal reflection of prism and suddenly dies Ripple, this evanescent wave returns again to after near interface material effect through interface certain depth, and reflection light can carry near interface material THz wave spectrum information, it is achieved thereby that the biological sample of sub-wavelength size to be carried out the technology of tera-hertz spectra detection.By public affairs Formula (1) understands, and the evanescent wave penetration depth of Terahertz attenuated total reflectance system, at some tens of pm, possesses and can detect near interface sample This spectral information, it is not necessary to sample is carried out any process, penetration depth and cellular layer thickness matching, extracellular water can be got rid of Interference, it is achieved non-marked, in real time, the advantage such as in-situ investigation, this technology be widely used to carbohydrate solutions hydrogen bond network, In protein glycosylation, biological macromolecule solns and the dynamic (dynamical) spectrum selective materials of intracellular aquation.
d p = λ / n p r i s m 2 π sin 2 θ - ( n e m / n p r i s m ) 2 - - - ( 1 )
The longitudinal section of the prism that the most various attenuated total reflectance systems built based on terahertz light spectrometer are used be triangle or It it is irregular polygon.On the one hand this needs to purchase special prism bracket, in transformation terahertz light spectrometer transmission mode parallel enter Penetrate light oblique incident ray at a certain angle, overall light path need to be adjusted, it is impossible to realize convenient attenuated total reflectance system of building Purpose.On the other hand the prism of irregular polygon because of volume relatively large, more expend make material, prism surface evanescent wave Limitation is distributed in subregion, when being applied to different biological molecules (protein, nucleic acid) solution detection analysis, and invalid sample Consumption is the most, easily causes the rarest, the waste of the relatively low biological macromolecule solns of abundance especially.Simultaneously because internal light path Longer, need light path time delay is adjusted on a large scale.
Summary of the invention
In view of this, the drawbacks described above that the present invention is directed to prior art provides a kind of Terahertz based on Dove prism fast construction to decline Subtracting full reflected system, the longitudinal section of the prism in this system is isosceles trapezoid;Employing can make parallel input light realize at interface, base The high-index material of total reflection is made;By its three logical light mirror polish, only parallel for prism putting the most just can need to be realized On tradition terahertz light spectrometer transmission mode, fast construction is for the attenuated total reflectance system of sample tera-hertz spectra detection, significantly Degree reduces prism volume, and prism surface all covers evanescent wave simultaneously, significantly reduces sample consumption.
It is an object of the invention to be achieved through the following technical solutions:
A kind of Terahertz attenuated total reflectance system based on Dove prism fast construction, including the femto-second laser set gradually, divides Bundle device, Terahertz generator, prism, Terahertz receptor and signal processing system, described prism be shaft section be isosceles trapezoid Dove prism, described Dove prism uses the High Resistivity Si material of high index of refraction to make, described beam splitter and Terahertz receptor it Between be additionally provided with reflecting mirror, described Terahertz generator, Terahertz receptor are by the photoconductive antenna being wholely set and hemispherical Silicon lens forms,
Laser beam splitter, is decomposed into two bundle laser by the light beam that femto-second laser produces, a branch of as pump beam, through postponing Line acts on Terahertz generator;A branch of act on Terahertz receptor as coherent probe beam through reflecting mirror,
Terahertz generator, is used for producing THz wave,
Terahertz receptor, is used for detecting THz wave,
Signal processing system, for THz wave generation and detection control, and the collection of sample terahertz wave signal and point Analysis.
Further, described femto-second laser is titanium sapphire femto-second laser.
Further, the outer surface of described reflecting mirror uses the coat of metal.
Further, the time delay device for adjusting the pumping light path time it is provided with between described beam splitter and Terahertz generator.
Further, the translation stage that can move at three dimensions, institute it are provided with between described Terahertz generator and Terahertz receptor The trapezoidal upper end stating Dove prism, is placed on described translation stage.
Further, the refractive index of described High Resistivity Si material more than 1.9, the resistance of every centimetre is more than 10k Ω.
Further, the waist of described isosceles trapezoid and the angle between going to the bottom are 20 °~70 °.
The invention has the beneficial effects as follows:
The present invention is the Dove prism of isosceles trapezoid by using shaft section, only need to the most just can be quick by parallel for prism putting Build the attenuated total reflectance system for the detection of sample tera-hertz spectra, it is not necessary to prism bracket supports, it is not required that overall light path Adjusting, can be greatly reduced and build time and materials consuming, prism surface all covers evanescent wave simultaneously, can significantly reduce tested Amount of samples, is beneficial to reduce use cost further.
Other advantages, target and the feature of the present invention will be illustrated to a certain extent in the following description, and To a certain extent, will be apparent to those skilled in the art based on to investigating hereafter, or can To be instructed from the practice of the present invention.The target of the present invention and other advantages can be realized by description below And acquisition.
Accompanying drawing explanation
In order to make the object, technical solutions and advantages of the present invention clearer, below in conjunction with accompanying drawing, the present invention is made into one The detailed description of step, wherein:
The schematic diagram of Fig. 1 Terahertz of the present invention attenuated total reflectance system;
Fig. 2 is the front view of Dove prism;
Fig. 3 is the top view of Fig. 2;
Fig. 4 is the time-domain spectroscopy peak value correlation curve figure using the present invention to measure 1.5% bovine serum albumin solution and water.
Detailed description of the invention
Hereinafter with reference to accompanying drawing, the preferred embodiments of the present invention are described in detail.Should be appreciated that preferred embodiment Only for the explanation present invention rather than in order to limit the scope of the invention.
As it is shown in figure 1, a kind of Terahertz attenuated total reflectance system based on Dove prism fast construction, including flying of setting gradually Second laser instrument 1, laser beam splitter 2, Terahertz generator 5, Dove prism 6, Terahertz receptor 8 and signal processing system 9, femto-second laser 1 specifically chosen titanium sapphire femto-second laser is as pumping and exploring laser light;Dove prism 6 specially axle cuts Face is isosceles trapezoid, uses the Terahertz prism made of High Resistivity Si material, and by three logical light mirror polish, can increase its printing opacity energy Power, owing to High Resistivity Si is minimum in the absorption of terahertz wave band, and refractive index is relatively big, it is advantageous to the resistance of every centimetre length is more than The High Resistivity Si material of 10k Ω, is additionally provided with reflecting mirror 3 between laser beam splitter 2 and Terahertz receptor 8,
Laser beam splitter 2, is decomposed into two bundle laser by the light beam that femto-second laser produces, a branch of as coherent probe beam, makees For being arranged on the reflecting mirror between beam splitter and Terahertz receptor;A branch of as pump beam, act on Terahertz and occur Device;
Terahertz receptor 8 and Terahertz generator 5 are by the detection photoconductive antenna of THz wave, hemispherical silicon lens composition;
Signal processing system 9, for THz wave generation and detection control, and the collection of sample terahertz wave signal and point Analysis.
Owing to the metals such as aluminum, silver, gold are all up to 99% to the reflectance of THz wave, so the coat of metal of reflecting element is permissible For any one in metal material, the reflecting element such as reflecting mirror used in the present invention, off-axis paraboloidal mirror all uses metal material Coating.
Further, the time delay device for adjusting the pumping light path time it is provided with between laser beam splitter 2 and Terahertz generator 5 4, concrete, time delay device 4 is made up of three reflecting mirrors as shown in the figure, certainly, can increase reflection according to concrete time delay needs Mirror quantity, it is also possible to be other any form of prior art.
Further, between Terahertz generator 5 and Terahertz receptor 8, it is provided with the translation stage 7 that can move at three dimensions, The trapezoidal upper end of Dove prism, is placed on translation stage 7, on the one hand can form Dove prism and support, on the other hand can adjust Dove prism particular location in systems so that it is there is more preferable mobility.
Further, in the present embodiment, the waist of isosceles trapezoid and the angle between going to the bottom are 20 °~70 °, more preferably 45 °.
Specific embodiment
Determine parameter: focus on incident spot size in the transmissive mode according to conventional terahertz light spectrometer and occur Terahertz at prism Occur the required angle conditions of total reflection to determine prism dimensions, and guarantee Terahertz launching spot size and prism side (cl face) Joining, as shown in Figure 2,3, each parameter is as follows:
Angle [alpha] is 45 °;Angle beta is 56.9 °;Angle Θ is 11.9 °;The a length of 1cm of a;The a length of 5cm of b;C length For 2.83cm;The a length of 2cm of h;The a length of 2cm of l;The a length of 2cm of a length of 2.83cm of c, l.
Build process: under terahertz light spectrometer transmission measurement pattern, directly places Terahertz Dove prism in translation stage central authorities position Put so that it is front and back sides direction keeps consistent with Terahertz transmitted light path.Change according to light path and suitably adjust light path time delay, for This optical path prism time delay is set to increase 500ps, i.e. realizes Terahertz attenuated total reflectance spectral measurement platform building, and whole process is time-consuming Within 1 minute, the Terahertz attenuated total reflectance system after building is as shown in Figure 1.
Measurement process: measure for biological macromolecule solns sample, draw 200 μ L sample drop tables on Dove prism with liquid-transfering gun Face, and make it cover whole surface, utilize signal processing system to obtain and store attenuated total reflectance spectrum time-domain signal.This enforcement Example is measured with 1.5% bovine serum albumin solution and aqueous solution, and Fig. 4 is shown in the contrast of its Terahertz attenuated total reflectance time domain peak, detection Time domain peak to bovine serum albumin solution is reported more less than aqueous solution time domain peak, difference about 4.7%, this and pertinent literature Unanimously, it was demonstrated that the whole Terahertz attenuated total reflectance system built can be used in biological spectrum detection, possesses retrofit process time-consuming Short, that sample consumption is few advantage.
Finally illustrating, above example is only in order to illustrate technical scheme and unrestricted, although with reference to preferably The present invention has been described in detail by embodiment, it will be understood by those within the art that, can be to the skill of the present invention Art scheme is modified or equivalent, and without deviating from objective and the scope of the technical program, it all should be contained at this In the middle of bright right.

Claims (7)

1. a Terahertz attenuated total reflectance system based on Dove prism fast construction, it is characterised in that: include that set gradually flies Second laser instrument, beam splitter, Terahertz generator, prism, Terahertz receptor and signal processing system, described prism is axle Cross section is the Dove prism of isosceles trapezoid, and described Dove prism uses the High Resistivity Si material of high index of refraction to make, described beam splitter And being additionally provided with reflecting mirror between Terahertz receptor, described Terahertz generator, Terahertz receptor are by being wholely set Photoconductive antenna and hemispherical silicon lens composition,
Laser beam splitter, is decomposed into two bundle laser by the light beam that femto-second laser produces, a branch of as pump beam, through delay line Act on Terahertz generator;A branch of act on Terahertz receptor as coherent probe beam through reflecting mirror,
Terahertz generator, is used for producing THz wave,
Terahertz receptor, is used for detecting and receiving THz wave,
Signal processing system, generation and detection for THz wave control, and the collection of sample terahertz wave signal and analysis.
A kind of Terahertz attenuated total reflectance system based on Dove prism fast construction the most according to claim 1, its feature exists In: described femto-second laser is titanium sapphire femto-second laser.
A kind of Terahertz attenuated total reflectance system based on Dove prism fast construction the most according to claim 1, its feature exists In: the outer surface of described reflecting mirror uses the coat of metal.
A kind of Terahertz attenuated total reflectance system based on Dove prism fast construction the most according to claim 1, its feature exists In: it is provided with the time delay device for adjusting the pumping light path time between described beam splitter and Terahertz generator.
A kind of Terahertz attenuated total reflectance system based on Dove prism fast construction the most according to claim 1, its feature exists In: it is provided with the translation stage that can move at three dimensions, described road prestige between described Terahertz generator and Terahertz receptor The trapezoidal upper end of prism, is placed on described translation stage.
A kind of Terahertz attenuated total reflectance system based on Dove prism fast construction the most according to claim 1, its feature exists In: the refractive index of described High Resistivity Si material more than 1.9, the resistance of every centimetre is more than 10k Ω.
A kind of Terahertz attenuated total reflectance system based on Dove prism fast construction the most according to claim 1, its feature exists It it is 20 °~70 ° in the waist of: described isosceles trapezoid and the angle between going to the bottom.
CN201610234800.9A 2016-04-15 2016-04-15 Rapidly established terahertz attenuation total reflection system based on Dove prism Pending CN105928898A (en)

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CN108458987A (en) * 2018-06-05 2018-08-28 天津大学 A kind of THz wave computer-aided tomography imaging device and method
CN109060729A (en) * 2018-10-29 2018-12-21 中国人民解放军陆军军医大学第附属医院 Transwell detection device and method based on Terahertz Technique of Attenuated Total Reflectance
CN109444085A (en) * 2018-12-18 2019-03-08 深圳先进技术研究院 A kind of near field THz wave spectrum imaging system and method
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CN110141248A (en) * 2019-05-21 2019-08-20 上海理工大学 Device and method based on decaying total reflection Terahertz dielectric spectra calibration blood sugar concentration
CN110553997A (en) * 2019-09-09 2019-12-10 中国人民解放军陆军军医大学第一附属医院 Early cancer detection method based on terahertz attenuated total reflection mode
CN110865043A (en) * 2019-11-22 2020-03-06 天津大学 Terahertz attenuated total reflection imaging device and method based on horizontal scanning mode
WO2020125437A1 (en) * 2018-12-18 2020-06-25 深圳先进技术研究院 Near-field terahertz wave spectral imaging system and method
CN114545585A (en) * 2022-02-23 2022-05-27 华太极光光电技术有限公司 Method for determining position between parabolic mirror and silicon prism
CN115825001A (en) * 2022-12-20 2023-03-21 北京环境特性研究所 Variable-angle terahertz frequency band attenuated total reflection material parameter measuring system

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CN107421915A (en) * 2017-09-08 2017-12-01 中国工程物理研究院流体物理研究所 A kind of living cells based on terahertz time-domain decay total reflection spectrum monitors experimental method in real time
CN108458987A (en) * 2018-06-05 2018-08-28 天津大学 A kind of THz wave computer-aided tomography imaging device and method
CN109060729A (en) * 2018-10-29 2018-12-21 中国人民解放军陆军军医大学第附属医院 Transwell detection device and method based on Terahertz Technique of Attenuated Total Reflectance
CN109060729B (en) * 2018-10-29 2019-08-20 中国人民解放军陆军军医大学第一附属医院 Transwell detection device and method based on Terahertz Technique of Attenuated Total Reflectance
CN109444084B (en) * 2018-11-05 2023-12-12 天津大学 Terahertz wave high-sensitivity imaging device based on dual modes
CN109444084A (en) * 2018-11-05 2019-03-08 天津大学 A kind of THz wave high sensitivity imaging device based on double mode
WO2020125437A1 (en) * 2018-12-18 2020-06-25 深圳先进技术研究院 Near-field terahertz wave spectral imaging system and method
CN109444085A (en) * 2018-12-18 2019-03-08 深圳先进技术研究院 A kind of near field THz wave spectrum imaging system and method
CN110141248A (en) * 2019-05-21 2019-08-20 上海理工大学 Device and method based on decaying total reflection Terahertz dielectric spectra calibration blood sugar concentration
CN110553997A (en) * 2019-09-09 2019-12-10 中国人民解放军陆军军医大学第一附属医院 Early cancer detection method based on terahertz attenuated total reflection mode
CN110865043A (en) * 2019-11-22 2020-03-06 天津大学 Terahertz attenuated total reflection imaging device and method based on horizontal scanning mode
CN114545585A (en) * 2022-02-23 2022-05-27 华太极光光电技术有限公司 Method for determining position between parabolic mirror and silicon prism
CN114545585B (en) * 2022-02-23 2024-04-26 华太极光光电技术有限公司 Method for determining position between parabolic mirror and silicon prism
CN115825001A (en) * 2022-12-20 2023-03-21 北京环境特性研究所 Variable-angle terahertz frequency band attenuated total reflection material parameter measuring system

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Application publication date: 20160907