CN105925522B - A kind of Exserohilum turcicum product spore culture medium and its application - Google Patents
A kind of Exserohilum turcicum product spore culture medium and its application Download PDFInfo
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- CN105925522B CN105925522B CN201610567969.6A CN201610567969A CN105925522B CN 105925522 B CN105925522 B CN 105925522B CN 201610567969 A CN201610567969 A CN 201610567969A CN 105925522 B CN105925522 B CN 105925522B
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Abstract
The present invention provides a kind of Exserohilum turcicum product spore culture medium and its application, contains following raw material components: 20 ~ 25 g of mannitol, 3 ~ 5 g of peptone, 40 ~ 60 g of fresh corn leaf, 30 ~ 50 g of sorghum grain, 14 ~ 16 g of agar powder in every liter of the Exserohilum turcicum product spore culture medium.Exserohilum turcicum is stained with sterile water with sterilizing writing brush and brushes away Exserohilum turcicum surface layer aerial hyphae in 25 DEG C of 10 ~ 15 d of dark culturing of the product spore culture medium, 25 DEG C of 72 h of alternation of light and darkness culture, that is, generates that a large amount of forms are consistent, the consistent conidium of maturity.Corn variety Disease Resistance Identification is carried out using the Exserohilum turcicum conidium that the culture medium culture generates, corn variety resource can be evaluated to the resistance level of leaf blight, this will play positive effect to the prevention and control of the leaf blight of corn.
Description
Technical field
The present invention relates to plant pathogenic fungi studying technological domains, specifically, being related to a kind of Exserohilum turcicum
(Exserohilum turcicum) product spore culture medium and its application.
Background technique
The leaf blight of corn (Northern Corn Leaf Blight) is also known as leaf spot, dead leaf disease, cecospora spot, is generation
The important leaf diseases of boundary various regions corn, be by Exserohilum turcicum (Exserohilum turcicum(Pass.) Leonard
Et Suggs) caused by (the also known as convex navel child spore bacterium of the big spot of corn).Exserohilum turcicum is mainly adhered to mycelia or conidium
It is overwintering in invalid tissue, borrow air-flow to travel on plant at the beginning of next year.Under the conditions of 20 ~ 25 DEG C of temperature, grown from spore both ends
Germ tube, attachment spore and intrusion silk, directly invade, 6 ~ 12 h can be completed, and 3 ~ 4 d can shape between epidermal cell or epidermal cell
At scab, scab generates conidium later, propagates with air-flow and rainwater, is infected repeatedly to the corn in field, cause disease
Evil eruption and prevalence.The disease is prevented and treated at present mainly to plant based on disease-resistant variety, and scientific and reasonable Variety distribution reduces First aggression
Source, the comprehensive technical measures such as chemical prevention.And in pathogenecity measurement, variety resistance identification and pesticide control screening test
In generally require how the largely conidiums manually cultivated are quickly obtained a large amount of conidiums very as development test material
Progress of research and success or failure are decide in big degree.
The prior art carries out Exserohilum turcicum frequently with potato dextrose agar (PDA) culture medium and produces spore culture, but
Since sporulation quantity is few, the further investigation of the research work such as disease-resistant variety breeding and Resistance resource screening is limited.Therefore, one is found
The culture medium that the easy suitable Exserohilum turcicum rapid, high volume of kind produces spore has great importance.
Summary of the invention
The purpose of the present invention is to provide a kind of product spore culture medium of Exserohilum turcicum, comprehensively utilize carbon source, nitrogen source and
Vitamin improves the sporulation quantity of Exserohilum turcicum.
In order to solve the above technical problems, technical solution of the present invention provides a kind of product spore culture medium of Exserohilum turcicum,
In every liter of the Exserohilum turcicum product spore culture medium contain following raw material components: 20 ~ 25 g of mannitol, 3 ~ 5 g of peptone,
40 ~ 60 g of fresh corn leaf, 30 ~ 50 g of sorghum grain, 14 ~ 16 g of agar powder.
Contain following raw material group in every liter of the product spore culture medium of the Exserohilum turcicum as a preferred technical solution,
Point: 25 g of mannitol, 4 g of peptone, 50 g of fresh corn leaf, 50 g of sorghum grain, 15 g of agar powder.
The sorghum grain is spare with cooking machine crushing, and fresh corn leaf clear water adds 20 times of corn leaf quality after cleaning
Clear water filters after boiling 30 ~ 40 min, and sorghum grain, mannitol, peptone and the agar powder of crushing are added in supernatant, heats molten
Solution adds water constant volume, adjusts pH value to 7.0,121 DEG C of 20 min of high pressure sterilization, the cooling big volume production spore of Exserohilum turcicum that obtains is trained
Support base.
Pathogen acquisition is from Sanming, Fujian Province, southern coequally maize leaf (acquisition time is 2014 ~ 2015 years), using Ke He
The verifying of family name's rule is observed the Morphological Characteristics of Their Pathogenic after isolating and purifying, and is connect with the resulting conidium of PDA culture medium culture
Kind plant, observes its disease symptom, and microscopy separates pathogen.Meanwhile measuring usExserohilum turcicum(Pass.) Leonard et Suggs DNA-ITS sequence (GenBank accession number is KX266811) is in Genbank
In be compared, withSetosphaeria turcicaThe homology of bacterial strain (accession number KR263032) is 99%.Therefore, will
Pathogen identification be Exserohilum turcicum [Exserohilum turcicum(Pass.) Leonard et Suggs].
The present invention also provides the production spore method of the Exserohilum turcicum product spore culture medium, method particularly includes: it will activate
Good Exserohilum turcicum, is beaten from its edge with 5 mm punch of diameter and takes bacterium dish, is then transferred to the leaf blight of corn
Bacterium product spore culture medium surface, 10 ~ 15 d of dark culturing at 25 DEG C, is stained with sterile water with sterilizing writing brush and brushes away Exserohilum turcicum surface layer
Aerial hyphae, 25 DEG C of 72 h of alternation of light and darkness culture generate that a large amount of forms are consistent, the consistent conidium of maturity.
The present invention also provides with the Exserohilum turcicum product spore culture medium corn variety Resistance To Helminthosporium Turcicum identification in
Using, method particularly includes: use sterilizing writing brush and sterile water by the Exserohilum turcicum on Exserohilum turcicum product spore culture medium surface
Every milliliter 1 ~ 5 × 10 is configured under conidium brush, after filtering5The conidial suspension of a spore, in corn variety 5 ~ 10
The leaf phase carries out plant spray inoculation, investigates incidence after stable disease, identifies the disease resistance of corn variety.
The product spore culture medium Easy dosing of Exserohilum turcicum provided by the invention, materials are convenient, strong operability, respectively
Using mannitol as carbon source, peptone is nitrogen source, provides Exserohilum turcicum and produces carbon, nitrogen required for spore, the life of addition
Vitamin in the long factor-sorghum grain adjusts microorganism eubolism, and the maize leaves of addition promote the production of Exserohilum turcicum
Spore.The product spore culture medium flat-plate bacterial colony of Exserohilum turcicum provided by the invention, which grows fine, produces spore ability is substantially better than routine
PDA culture medium, sporulation quantity is 4.3 ~ 23.1 times of PDA culture medium sporulation quantity of equivalent.The leaf blight of corn provided by the invention
The product spore culture medium of bacterium can be used for the research work such as subsequent disease-resistant variety breeding and Resistance resource screening.
Detailed description of the invention
Fig. 1 be bacterium colony growing state of the Exserohilum turcicum in different culture medium, wherein A be PDA culture medium, B be with
Than one, C be proportion two, D be proportion three, E be match four, F be match five；
Fig. 2 is production spore situation of the Exserohilum turcicum in different culture medium, and it is that two, C of proportion is that wherein A, which is one, B of proportion,
Proportion three, D be proportion four, E be match five, F be PDA culture medium；
Fig. 3 be colonial morphology of the Exserohilum turcicum in the product spore culture medium of optimization, hypha form, conidium and point
Raw sporophore form, wherein A is colonial morphology, and B is hypha form, and C is conidium form, and D is conidiophore form；
Fig. 4 is corn incidence after the conidium inoculation that Exserohilum turcicum product spore culture medium generates, and wherein A is clear
Water control, B are the conidial suspension inoculation for matching four, and figure C is scab local pictures.
Specific embodiment
Below in conjunction with the embodiment in the present invention, the technical solution in the embodiment in the present invention is carried out clear, complete
Whole description.Without departing from the spirit and essence of the present invention, the modification or replacement done to the present invention, belong to this hair
Bright scope.
Embodiment 1:
(1) it is formulated as follows the Exserohilum turcicum product spore culture medium (proportion one) of formula with clear water, contains in every liter following
Raw material components: 20 g of mannitol, 3 g of peptone, 40 g of fresh corn leaf, 30 g of sorghum grain, 14 g of agar powder.
(2) sorghum grain is spare with cooking machine crushing, fresh corn leaf clear water adds corn leaf quality 20 after cleaning
Clear water again filters after boiling 30 min, and sorghum grain, mannitol, peptone and the agar powder of crushing are added in supernatant, heats
Dissolution adds water to be settled to 1 L, adjusts pH value to 7.0,121 DEG C of 20 min of sterilizing, cooling obtains the big volume production of Exserohilum turcicum
Spore culture medium.
(3) Exserohilum turcicum being stored in 4 DEG C of refrigerators is taken out, is transferred to PDA culture medium (PDA culture medium: Ma Ling
200 g of potato, 20 g of glucose, 15 g of agar powder, 1 L of water) on activate, then by activated Exserohilum turcicum 5 mm of diameter
Punch is beaten from its edge and takes bacterium dish, then bacterium dish is transferred to the Exserohilum turcicum product spore culture medium surface, processing
3 repetitions are set, are control, 25 DEG C of 13 d(Fig. 1-B of dark culturing with PDA culture medium), sterile water, which is stained with, with sterilizing writing brush brushes away
The sterile water of 20 mL is added in Exserohilum turcicum surface layer aerial hyphae, 25 DEG C of 72 h of alternation of light and darkness culture, every ware, with sterilizing hair
Pen counts sporulation quantity, sporulation quantity under the Exserohilum turcicum conidium brush on Exserohilum turcicum product spore culture medium surface
It is 4.3 times of PDA culture medium, as shown in Fig. 2-A.
Embodiment 2:
(1) it is formulated as follows the Exserohilum turcicum product spore culture medium (proportion two) of formula with clear water, contains in every liter following
Raw material components: 25 g of mannitol, 5 g of peptone, 60 g of fresh corn leaf, 50 g of sorghum grain, 16 g of agar powder.
(2) sorghum grain is spare with cooking machine crushing, fresh corn leaf clear water adds corn leaf quality 20 after cleaning
Clear water again filters after boiling 40 min, and sorghum grain, mannitol, peptone and the agar powder of crushing are added in supernatant, heats
Dissolution adds water to be settled to 1 L, adjusts pH value to 7.0,121 DEG C of 20 min of sterilizing, cooling obtains the big volume production of Exserohilum turcicum
Spore culture medium.
(3) Exserohilum turcicum being stored in 4 DEG C of refrigerators is taken out, is transferred to PDA culture medium (PDA culture medium: Ma Ling
200 g of potato, 20 g of glucose, 15 g of agar powder, 1 L of water) on activate, then by activated Exserohilum turcicum 5 mm of diameter
Punch is beaten from its edge and takes bacterium dish, then bacterium dish is transferred to the Exserohilum turcicum product spore culture medium surface, processing
3 repetitions are set, are control, 25 DEG C of 10 d(Fig. 1-C of dark culturing with PDA culture medium), sterile water, which is stained with, with sterilizing writing brush brushes away
The sterile water of 20 mL is added in Exserohilum turcicum surface layer aerial hyphae, 25 DEG C of 72 h of alternation of light and darkness culture, every ware, with sterilizing hair
Pen counts sporulation quantity, sporulation quantity under the Exserohilum turcicum conidium brush on Exserohilum turcicum product spore culture medium surface
It is 12.7 times of PDA culture medium, as shown in fig. 2-b.
Embodiment 3:
(1) it is formulated as follows the Exserohilum turcicum product spore culture medium (proportion three) of formula with clear water, contains in every liter following
Raw material components: 20 g of mannitol, 5 g of peptone, 40 g of fresh corn leaf, 40 g of sorghum grain, 15 g of agar powder.
(2) sorghum grain is spare with cooking machine crushing, fresh corn leaf clear water adds corn leaf quality 20 after cleaning
Clear water again filters after boiling 35 min, and sorghum grain, mannitol, peptone and the agar powder of crushing are added in supernatant, heats
Dissolution adds water to be settled to 1 L, adjusts pH value to 7.0,121 DEG C of 20 min of sterilizing, cooling obtains the big volume production of Exserohilum turcicum
Spore culture medium.
(3) Exserohilum turcicum being stored in 4 DEG C of refrigerators is taken out, is transferred to PDA culture medium (PDA culture medium: Ma Ling
200 g of potato, 20 g of glucose, 15 g of agar powder, 1 L of water) on activate, then by activated Exserohilum turcicum 5 mm of diameter
Punch is beaten from its edge and takes bacterium dish, then bacterium dish is transferred to the Exserohilum turcicum product spore culture medium surface, processing
3 repetitions are set, are control, 25 DEG C of 12 d(Fig. 1-D of dark culturing with PDA culture medium), sterile water, which is stained with, with sterilizing writing brush brushes away
The sterile water of 20 mL is added in Exserohilum turcicum surface layer aerial hyphae, 25 DEG C of 72 h of alternation of light and darkness culture, every ware, with sterilizing hair
Pen counts sporulation quantity, sporulation quantity under the Exserohilum turcicum conidium brush on Exserohilum turcicum product spore culture medium surface
It is 6.5 times of PDA culture medium, as shown in Fig. 2-C.
Embodiment 4:
(1) it is formulated as follows the Exserohilum turcicum product spore culture medium (proportion four) of formula with clear water, contains in every liter following
Raw material components: 25 g of mannitol, 4 g of peptone, 50 g of fresh corn leaf, 50 g of sorghum grain, 15 g of agar powder.
(2) sorghum grain is spare with cooking machine crushing, fresh corn leaf clear water adds corn leaf quality 20 after cleaning
Clear water again filters after boiling 40 min, and sorghum grain, mannitol, peptone and the agar powder of crushing are added in supernatant, heats
Dissolution adds water to be settled to 1 L, adjusts pH value to 7.0,121 DEG C of 20 min of sterilizing, cooling obtains the big volume production of Exserohilum turcicum
Spore culture medium.
(3) Exserohilum turcicum being stored in 4 DEG C of refrigerators is taken out, is transferred to PDA culture medium (PDA culture medium: Ma Ling
200 g of potato, 20 g of glucose, 15 g of agar powder, 1 L of water) on activate, then by activated Exserohilum turcicum 5 mm of diameter
Punch is beaten from its edge and takes bacterium dish, then bacterium dish is transferred to the Exserohilum turcicum product spore culture medium surface, processing
3 repetitions are set, are control, 25 DEG C of 14 d(Fig. 1-E of dark culturing with PDA culture medium), sterile water, which is stained with, with sterilizing writing brush brushes away
The sterile water of 20 mL is added in Exserohilum turcicum surface layer aerial hyphae, 25 DEG C of 72 h of alternation of light and darkness culture, every ware, with sterilizing hair
Pen counts sporulation quantity, sporulation quantity under the Exserohilum turcicum conidium brush on Exserohilum turcicum product spore culture medium surface
It is 23.1 times of PDA culture medium, as shown in Fig. 2-D, Fig. 3.
Embodiment 5:
(1) it is formulated as follows the Exserohilum turcicum product spore culture medium (proportion five) of formula with clear water, contains in every liter following
Raw material components: 23 g of mannitol, 4 g of peptone, 50 g of fresh corn leaf, 40 g of sorghum grain, 15 g of agar powder.
(2) sorghum grain is spare with cooking machine crushing, fresh corn leaf clear water adds corn leaf quality 20 after cleaning
Clear water again filters after boiling 35 min, and sorghum grain, mannitol, peptone and the agar powder of crushing are added in supernatant, heats
Dissolution adds water to be settled to 1 L, adjusts pH value to 7.0,121 DEG C of 20 min of sterilizing, cooling obtains the big volume production of Exserohilum turcicum
Spore culture medium.
(3) Exserohilum turcicum being stored in 4 DEG C of refrigerators is taken out, is transferred to PDA culture medium (PDA culture medium: Ma Ling
200 g of potato, 20 g of glucose, 15 g of agar powder, 1 L of water) on activate, then by activated Exserohilum turcicum 5 mm of diameter
Punch is beaten from its edge and takes bacterium dish, then bacterium dish is transferred to the Exserohilum turcicum product spore culture medium surface, processing
3 repetitions are set, are control, 25 DEG C of 15 d(Fig. 1-F of dark culturing with PDA culture medium), sterile water, which is stained with, with sterilizing writing brush brushes away
The sterile water of 20 mL is added in Exserohilum turcicum surface layer aerial hyphae, 25 DEG C of 72 h of alternation of light and darkness culture, every ware, with sterilizing hair
Pen counts sporulation quantity, sporulation quantity under the Exserohilum turcicum conidium brush on Exserohilum turcicum product spore culture medium surface
It is 12.9 times of PDA culture medium, as shown in Fig. 2-E.
Embodiment 6:
The conidium that embodiment 4 obtains is configured to every milliliter 1 ~ 5 × 105The conidial suspension of a spore, to
Spray inoculation is carried out when corn length to 5 ~ 10 leaf phase, removal shading non-woven fabrics after 24 h of overlay film dark moisturizing culture after inoculation, 48
Film moisturizing culture is taken off after h, is investigated incidence after 5 ~ 7 d stable diseases, is evaluated the disease resistance of corn variety, as shown in Figure 4.
Described above is only the preferred embodiment of the present invention, for those skilled in the art,
Under the premise of not departing from the technology of the present invention principle, made modification and modification all should belong to covering scope of the invention.
Claims (5)
1. a kind of Exserohilum turcicum product spore culture medium, it is characterised in that: every liter of the Exserohilum turcicum product spore culture medium
In raw material components are as follows: supernatant that 20 ~ 25 g of mannitol, 3 ~ 5 g of peptone, 40 ~ 60 g fresh corn leaves are boiled through clear water,
30 ~ 50 g of sorghum grain, 14 ~ 16 g of agar powder；The supernatant is plus after 20 times of corn leaf quality of clear water boils 30 ~ 40 min
Filtering obtains.
2. a kind of Exserohilum turcicum product spore culture medium according to claim 1, it is characterised in that: the culture medium is every
Raw material components in liter are as follows: supernatant, the sorghum grain that 25 g of mannitol, 4 g of peptone, 50 g fresh corn leaves are boiled through clear water
50 g, 15 g of agar powder.
3. a kind of preparation method of Exserohilum turcicum product spore culture medium according to claim 1 or 2, it is characterised in that:
Clear water is cleaned after fresh corn leaf is weighed, and is filtered after adding 20 times of corn leaf quality of clear water to boil 30 ~ 40 min, in supernatant
Sorghum grain, mannitol, peptone and the agar powder crushed is added, is heated to agar powder dissolution, adds water constant volume, adjust pH value to
7.0, packing, 121 DEG C of 20 min of high pressure sterilization.
4. a kind of Exserohilum turcicum product spore culture medium as described in claim 1 is in corn variety to the disease-resistant of the leaf blight of corn
Property identification in application.
5. application according to claim 4, it is characterised in that: method particularly includes: by activated Exserohilum turcicum, use
5 mm punch of diameter is beaten from its edge takes bacterium dish, then bacterium dish is transferred to the Exserohilum turcicum product spore culture medium table
Face, 25 DEG C dark culturing 10 ~ 15 days, be stained with sterile water with sterilizing writing brush and brush away Exserohilum turcicum surface layer aerial hyphae, 25 DEG C of light
Dark alternate culture 72 hours, with sterilizing writing brush and sterile water by the leaf blight of corn on Exserohilum turcicum product spore culture medium surface
Under bacterium conidium brush, every milliliter 1 ~ 5 × 10 is configured to after filtering5The conidial suspension of a spore carries out plant
Spray inoculation investigates incidence, disease resistance of the identification corn variety to the leaf blight of corn after stable disease.
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Families Citing this family (7)
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|---|---|---|---|---|
| CN108251497A (en) * | 2018-04-04 | 2018-07-06 | 广西农业职业技术学院 | A kind of corn southern leaf blight efficient disease-resistance identification inoculum and preparation method thereof |
| CN108456712A (en) * | 2018-04-04 | 2018-08-28 | 广西农业职业技术学院 | A kind of leaf blight of corn efficient disease-resistance identification inoculum and preparation method thereof |
| CN109042156B (en) * | 2018-07-18 | 2021-06-08 | 福建省农业科学院植物保护研究所 | Artificial inoculation method for northern leaf blight of greenhouse maize |
| CN109220377A (en) * | 2018-09-07 | 2019-01-18 | 山东中农天泰种业有限公司 | A kind of corn breeding method of anti-tikka class disease |
| CN110305879B (en) * | 2019-07-10 | 2022-03-29 | 吉林大学 | Maize small leaf spot pathogen ChCDC3 gene and application thereof |
| CN115777462A (en) * | 2022-11-19 | 2023-03-14 | 福建省农业科学院植物保护研究所 | Artificial inoculation method of indoor corn seedling-stage northern leaf blight |
| CN115927010A (en) * | 2022-12-07 | 2023-04-07 | 福建省农业科学院植物保护研究所 | Method for improving activity and pathogenicity of corn northern leaf blight and application of method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101822272A (en) * | 2010-04-21 | 2010-09-08 | 中国农业科学院草原研究所 | Streptomyces griseoflavus and application thereof in biological prevention and control of plant diseases |
| CN103013860A (en) * | 2012-11-29 | 2013-04-03 | 吉林农业大学 | Preparation and application of biological control bacterial strain for diseases of ginseng plant |
| CN104962510A (en) * | 2015-07-14 | 2015-10-07 | 福建省农业科学院植物保护研究所 | Bipolaria maydis sporulation medium, and preparation method and application of bipolaria maydis sporulation medium |
-
2016
- 2016-07-19 CN CN201610567969.6A patent/CN105925522B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101822272A (en) * | 2010-04-21 | 2010-09-08 | 中国农业科学院草原研究所 | Streptomyces griseoflavus and application thereof in biological prevention and control of plant diseases |
| CN103013860A (en) * | 2012-11-29 | 2013-04-03 | 吉林农业大学 | Preparation and application of biological control bacterial strain for diseases of ginseng plant |
| CN104962510A (en) * | 2015-07-14 | 2015-10-07 | 福建省农业科学院植物保护研究所 | Bipolaria maydis sporulation medium, and preparation method and application of bipolaria maydis sporulation medium |
Non-Patent Citations (3)
| Title |
|---|
| 烟草赤星病菌分生孢子产生条件的研究;马贵龙等;《吉林农业大学学报》;20061231;第610-618页 * |
| 玉米大斑病流行过程重要环节的初步定量研究;杨信东 等;《吉林农业大学学报》;19880531;第6-10页 * |
| 黑龙江省西部地区玉米大斑病菌生理小种鉴定及生物学特性分析;浦子钢;《黑龙江农业科学》;20121231;第45-50页 * |
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