CN105924489A - Method for extracting platycodin in Radix Platycodonis through ASE - Google Patents
Method for extracting platycodin in Radix Platycodonis through ASE Download PDFInfo
- Publication number
- CN105924489A CN105924489A CN201610263372.2A CN201610263372A CN105924489A CN 105924489 A CN105924489 A CN 105924489A CN 201610263372 A CN201610263372 A CN 201610263372A CN 105924489 A CN105924489 A CN 105924489A
- Authority
- CN
- China
- Prior art keywords
- extraction
- radix platycodonis
- extracting
- ase
- platycodin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07J—STEROIDS
- C07J63/00—Steroids in which the cyclopenta(a)hydrophenanthrene skeleton has been modified by expansion of only one ring by one or two atoms
- C07J63/008—Expansion of ring D by one atom, e.g. D homo steroids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
- C07H1/08—Separation; Purification from natural products
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
- C07H15/20—Carbocyclic rings
- C07H15/24—Condensed ring systems having three or more rings
- C07H15/256—Polyterpene radicals
Abstract
The invention provides a method for extracting platycodin in Radix Platycodonis through ASE, and belongs to the technical field of chemical extraction. The invention aims at providing the method for extracting platycodin in Radix Platycodonis through ASE, having a high extraction efficiency and being simple to operate. The method comprises the following steps: 1, taking Radix Platycodonis, grinding Radix Platycodonis, taking 3g of the ground Radix Platycodonis, placing the 3g of the Radix Platycodonis in a 20mL ASE350 extraction pool, and extracting the above sample with ethyl acetate, wherein the extraction temperature is 65DEG C, the static extraction time is 5min, the extraction frequency is 1, and the flushing volume is 80%; 2, extracting the sample with n-butanol, wherein the extraction temperature is 70DEG C, the static extraction time is 3min, and the extraction frequency is 2; and 3, mixing above obtained two extract liquids, and carrying out reduced pressure evaporation on the obtained extract liquid mixture until the extract liquid mixture is dry.
Description
Technical field
The present invention provides the method for platycodin in a kind of ASE extraction Radix Platycodonis；Belong to chemistry to carry
Take technical field.
Background technology
Radix Platycodonis, another name burden flower, Radix Platycodonis, mitral flower, be herbaceos perennial, stem
High 20～120 centimetres, generally without hair, even close by undercoat, not branch, few upper part
Branch.Leaf is the most verticillate, verticillate to the whole alternate of part, stockless or have extremely short handle, blade
Avette, ovum shape is oval to lanceolar, and leaf is avette or ovum shape lanceolar, flower skipper or
Dark violet white, can make ornamental flower；Its root can be used as medicine, and has eliminating phlegm and stopping cough, lung qi dispersing, evacuation of pus
Deng effect, traditional Chinese medical science common medicine.Often pickled as brined vegetable in Northeast Area of China, in Korea half
Island is used to make Pickles, and what local folk rhyme " Radix Platycodonis ballad " was described is exactly this plant.
Radix Platycodonis dry root is cleaned, dry, pulverizing, tinning, 70% ethanol are solvent, adds
Circumfluence distillation three times, each 2 hours, merges three extracting solution, reclaims ethanol to without alcohol
After, concentration and evaporation water, to extractum, is spray-dried to obtain Radix Platycodonis extract powder, extracts yield one
As be 15%, size-reduced, sieve (100 mesh), mixing, pack to obtain finished product.
Summary of the invention
For above-mentioned deficiency, it is an object of the invention to provide a kind of extraction efficiency high, operation letter
The method of platycodin in single ASE extraction Radix Platycodonis.
For solving above-mentioned technical problem, the technical scheme that the present invention provides is as follows:
In a kind of ASE extraction Radix Platycodonis, the method for platycodin, comprises the steps: successively
1) Radix Platycodonis is taken, finely ground, take 3g and be placed in 20mLASE350 abstraction pool, use acetic acid
Sample is extracted by ethyl ester, and described Extracting temperature is 65 DEG C, and static extraction time is
5min, extraction time is 1 time, flush volume 80%；
2) again with n-butyl alcohol as Extraction solvent, Extracting temperature is 70 DEG C, static extraction time
For 3min, extraction time is 2 times；
3) twice extracting solution is merged, evaporated under reduced pressure and get final product.
Compared with prior art, the technical scheme extraction efficiency that the present invention provides is high, has extracted
Entirely, favorable repeatability.
Detailed description of the invention
Below in conjunction with detailed description of the invention, the claim of the present invention is done the most in detail
Illustrate, but do not constitute any limitation of the invention, any in the claims in the present invention protection
The amendment of the limited number of time that scope is made, still within the claims of the present invention.
Involved in the present invention to percentage composition concentration, in addition to specified otherwise, solute is liquid
Be volumetric concentration, solute be solid be mass concentration.
Embodiment 1
1) Radix Platycodonis is taken, finely ground, take 3g and be placed in 20mLASE350 abstraction pool, use acetic acid
Sample is extracted by ethyl ester, and described Extracting temperature is 65 DEG C, and static extraction time is
5min, extraction time is 1 time, flush volume 80%, purge time 50s；
2) again with n-butyl alcohol as Extraction solvent, Extracting temperature is 70 DEG C, static extraction time
For 3min, extraction time is 2 times, purge time 50s；
3) twice extracting solution is merged, evaporated under reduced pressure and get final product, extract methanol is dissolved
After, contrast with standard spectrogram, find consistent with Platycodin D reference colour spectrogram, extract
Rate 35.28%.
Claims (1)
1. the method for platycodin in an ASE extraction Radix Platycodonis, it is characterised in that successively
Comprise the steps:
1) Radix Platycodonis is taken, finely ground, take 3g and be placed in 20mLASE350 abstraction pool, use acetic acid
Sample is extracted by ethyl ester, and described Extracting temperature is 65 DEG C, and static extraction time is
5min, extraction time is 1 time, flush volume 80%；
2) again with n-butyl alcohol as Extraction solvent, Extracting temperature is 70 DEG C, static extraction time
For 3min, extraction time is 2 times；
3) twice extracting solution is merged, evaporated under reduced pressure and get final product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610263372.2A CN105924489A (en) | 2016-04-26 | 2016-04-26 | Method for extracting platycodin in Radix Platycodonis through ASE |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610263372.2A CN105924489A (en) | 2016-04-26 | 2016-04-26 | Method for extracting platycodin in Radix Platycodonis through ASE |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105924489A true CN105924489A (en) | 2016-09-07 |
Family
ID=56837171
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610263372.2A Pending CN105924489A (en) | 2016-04-26 | 2016-04-26 | Method for extracting platycodin in Radix Platycodonis through ASE |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105924489A (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101320738B1 (en) * | 2011-06-10 | 2013-10-21 | 강릉원주대학교산학협력단 | The method of extracting carotinoid pigments of micro algae and composition comprising the extracted fucoxanthin for preventing or treating obesity or diabetes |
| CN104819883A (en) * | 2015-05-12 | 2015-08-05 | 广西壮族自治区梧州食品药品检验所 | Method for extracting gastrodine from gastrodia elata |
| CN104876988A (en) * | 2015-05-12 | 2015-09-02 | 广西壮族自治区梧州食品药品检验所 | Method for extracting linarin from wild chrysanthemum flower |
| CN104873563A (en) * | 2015-04-29 | 2015-09-02 | 吉林农业大学 | Preparation method of platycodon root stem leaf saponin and application of platycodon root stem leaf saponin in preparing anti-tumor medicines and health-care products |
| CN104892625A (en) * | 2015-05-12 | 2015-09-09 | 广西壮族自治区梧州食品药品检验所 | Method for extracting linderane from radix linderae |
| CN104892701A (en) * | 2015-05-12 | 2015-09-09 | 广西壮族自治区梧州食品药品检验所 | Method used for extracting hesperidin from pills used for promoting digestion |
| CN104897804A (en) * | 2015-05-12 | 2015-09-09 | 广西壮族自治区梧州食品药品检验所 | Method used for extracting naringin from rhizoma drynariae |
| CN104910225A (en) * | 2015-05-12 | 2015-09-16 | 广西壮族自治区梧州食品药品检验所 | Method for extracting baicalin from radix scutellariae |
| EP2937092A1 (en) * | 2014-04-24 | 2015-10-28 | PharmAlp SA | Anti-candida compositions and uses thereof |
-
2016
- 2016-04-26 CN CN201610263372.2A patent/CN105924489A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101320738B1 (en) * | 2011-06-10 | 2013-10-21 | 강릉원주대학교산학협력단 | The method of extracting carotinoid pigments of micro algae and composition comprising the extracted fucoxanthin for preventing or treating obesity or diabetes |
| EP2937092A1 (en) * | 2014-04-24 | 2015-10-28 | PharmAlp SA | Anti-candida compositions and uses thereof |
| CN104873563A (en) * | 2015-04-29 | 2015-09-02 | 吉林农业大学 | Preparation method of platycodon root stem leaf saponin and application of platycodon root stem leaf saponin in preparing anti-tumor medicines and health-care products |
| CN104819883A (en) * | 2015-05-12 | 2015-08-05 | 广西壮族自治区梧州食品药品检验所 | Method for extracting gastrodine from gastrodia elata |
| CN104876988A (en) * | 2015-05-12 | 2015-09-02 | 广西壮族自治区梧州食品药品检验所 | Method for extracting linarin from wild chrysanthemum flower |
| CN104892625A (en) * | 2015-05-12 | 2015-09-09 | 广西壮族自治区梧州食品药品检验所 | Method for extracting linderane from radix linderae |
| CN104892701A (en) * | 2015-05-12 | 2015-09-09 | 广西壮族自治区梧州食品药品检验所 | Method used for extracting hesperidin from pills used for promoting digestion |
| CN104897804A (en) * | 2015-05-12 | 2015-09-09 | 广西壮族自治区梧州食品药品检验所 | Method used for extracting naringin from rhizoma drynariae |
| CN104910225A (en) * | 2015-05-12 | 2015-09-16 | 广西壮族自治区梧州食品药品检验所 | Method for extracting baicalin from radix scutellariae |
Non-Patent Citations (4)
| Title |
|---|
| 刘平怀 等: "加速溶剂萃取(ASE)技术提取海南萝芙木活性成分", 《精细化工》 * |
| 姜立春 等: "桔梗总皂苷提取工艺的优化研究", 《江苏农业科学》 * |
| 陈军辉 等: "加速溶剂萃取技术在中药有效成分分析中的应用", 《色谱》 * |
| 靳学远 等: "超高压提取桔梗皂苷工艺条件的优化", 《南方农业学报》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101928642B (en) | Method for extracting and separating spice and medicinal components from whole plants of Dendranthema indicum | |
| CN101810658B (en) | Method for removing residual pesticide from ginseng | |
| CN105168285A (en) | Ginsenoside extracting solution and extracting method thereof | |
| CN105670795B (en) | A kind of extracting method of peppermint oil | |
| CN103583738B (en) | A kind of dispersing liver and promoting blood circulation blood stasis releasing tea cream and preparation method thereof | |
| CN105250180A (en) | Rose fresh water and preparation method and application thereof | |
| CN102604744B (en) | Extraction method of dendrobium essential oil | |
| Li et al. | Accumulation and dynamic trends of triterpenoid saponin in vegetative organs of Achyranthus bidentata | |
| CN105777825A (en) | Method for extracting platycodins in platycodon grandiflorus by virtue of ionic liquid | |
| CN108379316A (en) | A kind of extracting method of ginsenoside | |
| CN102516213B (en) | Sesquiterpene compound and preparation method and application thereof | |
| CN105924489A (en) | Method for extracting platycodin in Radix Platycodonis through ASE | |
| CN100389783C (en) | Extraction process of effective Chuanxiong component | |
| CN102948851B (en) | A kind of panax notoginseng healthcare beverage and processing technique thereof | |
| CN103583745B (en) | A kind of tonifying QI to produce body fluid tea cream and preparation method thereof | |
| CN104922247A (en) | Extraction method and application of ethyl acetate extract of Prismatomeris tetrandra | |
| CN102898285A (en) | Magnolol supercritical extraction method | |
| CN109423358A (en) | A kind of extracting method of Chinese small iris volatile oil | |
| Liu et al. | Beyond subsistence: Evidence for red rice beer in 8000-year old Neolithic burials, north China | |
| CN113750163A (en) | Artemisia annua essential oil and white tea essential oil for treating acne and composition thereof | |
| CN102552363A (en) | Preparation method for mother chrysanthemum extract | |
| CN113881503A (en) | Artemisia annua essential oil and white tea essential oil with anti-inflammatory effect, and their composition | |
| CN105028838A (en) | Acne removing cosmetic tea | |
| CN104383079B (en) | The preparation method of Herba Houttuyniae extract | |
| CN104208156A (en) | Method for simultaneously extracting schisandra chinensis volatile oil and lignans |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160907 |
|
| RJ01 | Rejection of invention patent application after publication |