CN105920029A - Sargassum fusiforme polysaccharide having functions of regulating immunity capacity and resisting oxidation and application of sargassum fusiforme polysaccharide in health care product - Google Patents
Sargassum fusiforme polysaccharide having functions of regulating immunity capacity and resisting oxidation and application of sargassum fusiforme polysaccharide in health care product Download PDFInfo
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- CN105920029A CN105920029A CN201610288923.0A CN201610288923A CN105920029A CN 105920029 A CN105920029 A CN 105920029A CN 201610288923 A CN201610288923 A CN 201610288923A CN 105920029 A CN105920029 A CN 105920029A
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- polysaccharide
- sargassum fusiforme
- fusiforme polysaccharide
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- PNNNRSAQSRJVSB-UHFFFAOYSA-N L-rhamnose Natural products CC(O)C(O)C(O)C(O)C=O PNNNRSAQSRJVSB-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/02—Algae
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention discloses sargassum fusiforme polysaccharide having functions of regulating immunity capacity and resisting oxidation; the sargassum fusiforme polysaccharide is characterized by consisting of fucoidan polysaccharide sulfate and sodium alginate, wherein the monosaccharide component of the sodium alginate consists of 40-45% of guluronic acid and 8-15% of mannuronic acid in percentage by mass; and the monosaccharide component of the fucoidan polysaccharide sulfate consists of 19-39% of fucose and 3-10% of galactose in percentage by mass. The sargassum fusiforme polysaccharide, as a raw material, can be used for preparing a health care product having the functions of regulating immunity capacity and resisting oxidation by virtue of a conventional production method in the pharmaceutical field, wherein the health care product is in the form of tablets or capsules. The sargassum fusiforme polysaccharide is relatively good in the functions of regulating immunity capacity and resisting oxidation; therefore, the sargassum fusiforme polysaccharide is capable of protecting a body from free radical injury.
Description
Technical field
The present invention relates to a kind of for regulating immunocompetence and anti-oxidation function Hijiki polysaccharide, further relate to preparation method and the application in health product of this Hijiki polysaccharide of this Hijiki polysaccharide.
Background technology
Oxidative stress is when body is by external source or endogenous stimulation; active bioactive molecule such as oxygen-derived free radicals and nitrogen free radical content raise; accelerate oxidation reaction; once beyond body to oxidation product Scavenging activity, each system of body runs load down, causes homergy disorderly; inflammatory reaction occurs; inducing the generation of multiple disease, such as: neurodegenerative diseases, diabetes, hypertension, atherosclerosis etc., therefore opposing oxidative stress is the necessary means of protection body normal function.In the research of anti-oxidation stress, many polysaccharose substances are as natural antioxidant, reduce the indexs such as monoamine oxidase, MAO (MAO) and malonaldehyde (MDA) content to rise, preventing cell ageing or necrosis that these oxidase or oxidation product too much cause, cell dysfunction, ability of cell proliferation declines, protease activity reduces, and then whole organism aging process, immunity degradation, cause the various diseases occurred.During removing free radical; many antioxidases play an important role; such as relevant superoxide dismutase (SOD), catalase (CAT) etc.; these antioxidases can reduce the damage that free radical causes, the injury that protection body causes from oxidative stress.There are many polysaccharose substances to be applied to clinic as immune drug simultaneously, promoted that immune cell function raises, such as lentinan, ganoderan, pachyman etc., and achieved good effect.
The Sargassum included in the Pharmacopoeia of the People's Republic of China is Sargassaceae plant or Sargassum fusiforme (Harv.) Setch, and it has history remote, the edibility of the Sargassum the most on the books such as Shennong's Herbal, Compendium of Material Medica and medical value.Sargassum has higher healthy nutritive value, such as effects such as blood fat reducing, antithrombotic, enhancing immunity, antioxidation.Japan is called " longevity greens/mustard green ", and annual a large amount of from China's import.The bulk composition of Sargassum is polysaccharide, and these polysaccharide components have been demonstrated have the biologic activity such as blood fat reducing blood glucose, antioxidation and enhancing immunity, polysaccharide of the prior art is generally compositions, different extraction processes can produce different components, corresponding polysaccharide component and active polysaccharide are also affected by, certainly, effect of its correspondence and application are the most different.
Summary of the invention
The invention discloses a kind of Hijiki polysaccharide, this Hijiki polysaccharide has preferably regulation immunocompetence and antioxidative effect, and protection body is not by radical damage.
The invention also discloses and Hijiki polysaccharide is had regulation immunocompetence and anti-oxidation function application in health product as a kind of.
For Hijiki polysaccharide itself, a kind of have regulation immunocompetence and the Hijiki polysaccharide of anti-oxidation function, it is characterized in that: include sulfated fucan and Algin, the monosaccharide component of Algin includes guluronic acid, 8%～the mannuronic acid of 15% that mass ratio is 40%～45%, and the monosaccharide component of sulfated fucan includes that mass ratio is 19～the fucose of 39% and 3～10% galactose.
Using above-mentioned Hijiki polysaccharide as raw material, according to the conventional production process of pharmaceutical field, preparation is for regulating immunocompetence and the tablet of anti-oxidation function or the health product of capsule.
The advantage that the present invention compared with prior art has following several respects: this Hijiki polysaccharide can take into account regulation immunocompetence and anti-oxidation function, polysaccharide component relative to common sulfated fucan, sulfated fucan and the component proportion of Algin, can have the effect of more preferable external removing free radical, realize regulation body immunity, meanwhile, Algin polysaccharide itself can have the effect of the phagocytic activity promoting macrophage, has certain antioxidant activity.This Hijiki polysaccharide makes health product as raw material, has broad application prospects in health product.
Accompanying drawing explanation
The impact on Hydroxyl radical-scavenging ability of the Hijiki polysaccharide in Fig. 1 present invention；
The impact on the RAW264.7 macrophage proliferation ability under oxidative stress of Fig. 2 Hijiki polysaccharide；
Fig. 3 is the Hijiki polysaccharide impact on natural aging mice CAT enzyme activity；
Fig. 4 is the Hijiki polysaccharide impact on natural aging mice SOD enzyme activity；
Fig. 5 is the Hijiki polysaccharide impact on natural aging mice MAO enzyme activity；
Fig. 6 is the Hijiki polysaccharide impact on natural aging mice MDA content；
Natural aging mice spleen index is affected by Fig. 7 Hijiki polysaccharide；
Fig. 8 is the impact on RAW264.7 macrophages phagocytic capacity of the variable concentrations Hijiki polysaccharide.
Detailed description of the invention
Below in conjunction with the accompanying drawings and embodiment, the detailed description of the invention of the present invention is described in further detail.Following example are used for illustrating the present invention, but are not limited to the scope of the present invention.
Disclosing the preparation method of above-mentioned Hijiki polysaccharide in the present embodiment, its preparation method is as follows:
1) fresh Sargassum fusiforme (Harv.) Setch is put in baking oven, 40～60 DEG C of bakings 24～48h, shreds into section, puts into and grinds 1～2min in pulverizer, obtains Sargassum fusiforme (Harv.) Setch algae powder；
2) according to Sargassum fusiforme (Harv.) Setch algae powder: 95% ethanol=1: 4 (m: v), backflow defat 3 times, each 2h, obtain defat Sargassum fusiforme (Harv.) Setch algae powder；
3) according to defat Sargassum fusiforme (Harv.) Setch algae powder: distilled water=1: 30 (m: v), 60～90 DEG C are boiled and carry 3 times, and the time is followed successively by 2～4h, merges 3 leach cooking liquids, concentrating under reduced pressure volume to 1/12, obtains concentrated solution；
4) adding 95% ethanol in concentrated solution to ethanol final concentration of 80%, room temperature precipitate with ethanol overnight, abandons supernatant, collects precipitation；
5) precipitation obtained is carried out solvent seasoning, use 95% ethanol, absolute ethanol washing to precipitate successively, obtain Hijiki polysaccharide；
6) above-mentioned Hijiki polysaccharide is put in vacuum drying oven, 40～60 DEG C of decompression dryings, obtain the Hijiki polysaccharide with specific components.
Obtaining milk yellow powder according to the method described above, Hijiki polysaccharide yield is 18～25%, and polysaccharide component content is 75～85% after measured.
In the present embodiment, it is applied to the Hijiki polysaccharide of above-mentioned specific components regulate immunocompetence and anti-oxidation function, Hijiki polysaccharide includes sulfated fucan, Algin, the monosaccharide component of Algin includes the guluronic acid that mass ratio is 40%～45%, the mannuronic acid of 8%～15%, the monosaccharide component of sulfated fucan includes that mass ratio is 19～the fucose of 39% and 3～10% galactose, also include other polysaccharide, the monosaccharide of other polysaccharide consists of: rhamnose 3～5%, glucuronic acid 1～4%, xylose 4～8%, mannose 3～5%.
As it is shown in figure 1, the component proportion of the employing present invention relative to the polysaccharide component of common sulfated fucan, sulfated fucan and Algin, it is possible to there is the effect removing free radical outside more preferable purged body, it is achieved regulation body immunity.
Fig. 2 discloses the Hijiki polysaccharide impact on macrophage proliferation ability.
The present embodiment is also directed to Hijiki polysaccharide the regulation immunocompetence of mouse aging and anti-oxidation efficacy has been done following experiment:
Choose 8 old ICR mice, the male and female mice with the Hijiki polysaccharide of 100mg/kg/d gavage respectively, mouse liver as material, often group n=6～10, result such as Fig. 3 to Fig. 8.
The method of the present invention is simple to operate, the commercial production of proper scale type, uses nontoxic raw material, safer reliably.Confirm that hydroxy radical is had preferable Scavenging activity and Scavenging activity to be proportionate with Hijiki polysaccharide ability by Hijiki polysaccharide through above-mentioned experiment.Mtt assay is used to detect Hijiki polysaccharide under 490nm to by H2O2The impact of the RAW264.7 macrophage proliferation ability of oxidative damage, result shows that the Hijiki polysaccharide of low concentration can stop H2O2Inhibitory action to macrophage proliferation, its effect is better than lentinan.Phagocytosis dimethyl diaminophenazine chloride test result indicate that Hijiki polysaccharide can promote the phagocytic activity of macrophage, and its effect is good compared with lentinan；The raw material being gavage mice with 100mg/kg/d Hijiki polysaccharide solution, result display Hijiki polysaccharide can promote SOD and the CAT enzyme activity in the spleen index of mouse aging and mouse liver, reduce MDA content and MAO enzyme activity in mouse liver, show that Hijiki polysaccharide can be by improving immunity of organisms and antioxidase activity, stop oxidation reaction, effectively remove free radical and suppress body lipid peroxidation, reduce the accumulation reducing MDA, thus play internal antioxidative effect.In sum, the Hijiki polysaccharide that this method prepares can become enhancing immunoloregulation function and the health product of anti-oxidation function.
The above is only the preferred embodiment of the present invention; it should be pointed out that, for those skilled in the art, on the premise of without departing from the technology of the present invention principle; can also make some improvement and modification, these of above-mentioned hypothesis improve with modification with should be regarded as protection scope of the present invention.
Claims (2)
1. one kind has regulation immunocompetence and the Hijiki polysaccharide of anti-oxidation function, it is characterised in that: include fucoidin sulfur
Acid esters and Algin, the monosaccharide component of Algin includes the manna alditol of guluronic acid, 8%～15% that mass ratio is 40%～45%
Acid, the monosaccharide component of sulfated fucan includes that mass ratio is 19～the fucose of 39% and 3～10% galactose.
2., using the Hijiki polysaccharide in claim 1 as raw material, according to the conventional production process of pharmaceutical field, preparation is used for adjusting
Save immunocompetence and the tablet of anti-oxidation function or the health product of capsule.
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| CN201610288923.0A CN105920029A (en) | 2016-04-28 | 2016-04-28 | Sargassum fusiforme polysaccharide having functions of regulating immunity capacity and resisting oxidation and application of sargassum fusiforme polysaccharide in health care product |
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| CN201610288923.0A CN105920029A (en) | 2016-04-28 | 2016-04-28 | Sargassum fusiforme polysaccharide having functions of regulating immunity capacity and resisting oxidation and application of sargassum fusiforme polysaccharide in health care product |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108391820A (en) * | 2018-01-22 | 2018-08-14 | 温州大学苍南研究院 | A kind of Hijiki polysaccharide with bowel relaxing functions |
| CN110218262A (en) * | 2019-05-20 | 2019-09-10 | 浙江工业大学 | Application of the low sulphated heteroglycan rich in glucuronic acid in brown alga source in preparation treatment diabetes B drug |
| CN111096976A (en) * | 2020-01-09 | 2020-05-05 | 中国海洋大学 | Application of L-oligoguluronate in preparing medicine and functional food for delaying skin aging and resisting immune inflammation |
Citations (3)
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| CN108391820A (en) * | 2018-01-22 | 2018-08-14 | 温州大学苍南研究院 | A kind of Hijiki polysaccharide with bowel relaxing functions |
| CN110218262A (en) * | 2019-05-20 | 2019-09-10 | 浙江工业大学 | Application of the low sulphated heteroglycan rich in glucuronic acid in brown alga source in preparation treatment diabetes B drug |
| CN110218262B (en) * | 2019-05-20 | 2021-05-11 | 浙江工业大学 | Application of low-sulfated heteroglycan rich in glucuronic acid and derived from brown algae in preparation of medicines for treating type 2 diabetes |
| CN111096976A (en) * | 2020-01-09 | 2020-05-05 | 中国海洋大学 | Application of L-oligoguluronate in preparing medicine and functional food for delaying skin aging and resisting immune inflammation |
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