CN105918961A - Preparation method of taro functional fermented food - Google Patents

Preparation method of taro functional fermented food Download PDF

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Publication number
CN105918961A
CN105918961A CN201610271495.0A CN201610271495A CN105918961A CN 105918961 A CN105918961 A CN 105918961A CN 201610271495 A CN201610271495 A CN 201610271495A CN 105918961 A CN105918961 A CN 105918961A
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taro
preparation
monascus
fermented food
fermentation
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Inventor
吕旭聪
刘斌
贾瑞博
林占熺
李燕
周文斌
陈竞豪
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Fujian Agriculture and Forestry University
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Fujian Agriculture and Forestry University
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/121Brevis
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/129Cornyiformis
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/169Plantarum
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/21Streptococcus, lactococcus
    • A23V2400/231Lactis
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/31Leuconostoc
    • A23V2400/321Mesenteroides
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/41Pediococcus
    • A23V2400/413Acidilactici
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/41Pediococcus
    • A23V2400/427Pentosaceus

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  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The present invention discloses a preparation method of taro functional fermented food and relates to the field of microbial fermentation and biological medicine. The preparation method of the taro functional fermented food is as follows: taros are used as main raw materials, monascus and lactic acid bacteria are used as the fermented strains, and the taro functional fermented food is obtained by the fermentation process. The preparation process includes the steps: taro fermentation medium preparation, monascus liquid strain preparation, lactic acid bacterium liquid strain preparation, inoculation, mixed bacterium fermentation and culture, and finished product preparation. The taro functional fermented food prepared by the method is rich in taro polysaccharides, monacolin K, gamma aminobutyric acids and monascorubin, and low in citrinin content. The fermented product produced by the drying and crushing can be directly used as the functional food with the functions of regulating blood sugar and blood fat and improving the gastrointestinal tract function.

Description

A kind of preparation method of the functional fermented food of taro
Technical field
The invention belongs to fermentable and biomedicine field, be specifically related to a kind of functional fermented food of taro Preparation method.
Background technology
Monascus (MonasucsSpp.) it is one of China useful fungi being applied to food processing the earliest.About red colouring agent for food, also used as a Chinese medicine Medical value, Ming Dynasty's Li Shizhen (1518-1593 A.D.) describes in Compendium of Material Medica: " red colouring agent for food, also used as a Chinese medicine temperature taste is sweet, nontoxic.Main help digestion, invigorate blood circulation, invigorating the spleen, Dry stomach, controls dysentery, Xia Shuigu." " book on Chinese herbal medicine to roll up four through solution " description: " red colouring agent for food, also used as a Chinese medicine steams with plain rice and affixes one's name to into redness, has same gas with ying blood The reason asked mutually;Can rise blood and invigorating the spleen, spleen is good for, then for its rule liquid of stomach row, stomach is dry.Control dysentery person, there to be the merit helped digestion of invigorating blood circulation Also;Lower water paddy person, temperature reaches the power of liver taste sweet benefit spleen also ... ".Modern medicine study shows to produce in Monascus sweat Multiple secondary metabolite, has the multiple physiological active functionses such as anti-oxidant, reducing blood lipid, norcholesterol, prevention and cure of cardiovascular disease.
Lactic acid bacteria (lactic acid bacteria, LAB) is that a class can utilize fermentable carbohydrate to produce in a large number The common name of the bacterium of lactic acid.Lactic acid bacteria is possible not only to improve nutritive value of food, improves flavour of food products, improves food preservation And added value, and the special physiological of lactic acid bacteria activity and trophic function, the most day by day cause the attention of people.Numerous studies data Showing, lactic acid bacteria can promote growth of animal, regulation composition of gut flora, maintenance microecological balance, thus improves intestines and stomach merit , food digestion rate and biological value can be improved, reduce serum cholesterol, control endotoxin, spoilage organisms growth in suppression enteron aisle, with And improve immunity of organisms etc..
Taro (English name: taro;Latin name:Colocasia esculenta;Another name space pole stalwart, blue or green, soil sesame, hair taro) It is commonly called as outside taro, belongs to TianXing section herbaceous plant.Taro contains abundant MS, and its key component is mucopolysaccharide, can strengthen The immunity of body, improves the resistivity of body.And study and show that taro polysaccharide has good antioxidation.Modern Pharmacology analysis shows that taro also has the effects such as lowering blood pressure and blood fat, useful for preventing and treating cardiovascular and cerebrovascular diseases.If able to red The nutritive effect of aspergillus and the physiological function of lactic acid bacteria and taro organically combines, and makes fermented product rich in multiple efficacies Composition, it will make the functional tunning of taro have the highest edible and medical value.At present, relevant Monascus fermenting and producing The research report of monascorubin, Monascus anka Nakazawa et sato filament, MonacolinK and reduction citrinin content etc. is more.But, the most not yet See the report utilizing Monascus and lactobacillus mixed fermentation taro to prepare the functional fermented food of taro with multi-efficiency.
Summary of the invention
The invention provides a kind of side utilizing Monascus and lactobacillus mixed fermentation taro to prepare functional fermented food Method.On the one hand Monascus utilize taro rich in starch as culture medium, it is also possible to a large amount of production has reducing blood lipid, anti-artery congee The metabolite Lovastatin of the effect such as sample hardening and monascorubin;On the other hand widow is produced after taro starch is fermented by Monascus Sugar and glucose promote the growth and breeding of lactic acid bacteria, and lactic acid bacteria gives the good digestibility of taro fermented product and peculiar flavour. Taro Monascus and lactobacillus mixed fermentation product may be used for producing regulation blood sugar, blood fat and improving the function of gastrointestinal function Property fermented food.
The above-mentioned purpose of the present invention is achieved by the following technical solution:
The preparation method of the functional fermented food of a kind of taro, is prepared from by following steps:
(1) taro fermentation medium: add ammonium chloride 0.01-0.4 kg, maltose 0.01-1.0 kg in every 100 kg taros, Magnesium sulfate 0.001-0.1 kg, dipotassium hydrogen phosphate 0.001-0.1 kg, water 50-150 L;
(2) Monascus liquid strain: Monascus bacterial strain, after wort agar slant medium activation culture 3-6 days, accesses liquid Body Shake flask medium carries out liquid state fermentation cultivation and prepares Monascus liquid strain;
(3) lactic acid bacteria liquid strain: lactic acid bacteria strains, after MRS agar slant culture-medium anaerobism activation culture 1-2 days, accesses liquid Body lactic acid bacteria spread cultivation culture medium carry out liquid anaerobic fermentation cultivate preparation lactic acid bacteria liquid strain;
(4) preparation of the functional fermented food of taro: on the basis of the weight of the taro culture medium described in step (1), by weight Monascus liquid strain 1-20% described in percentages addition step (2), the lactic acid bacteria liquid strain 1-described in step (3) 10%, fermented and cultured 5-8 days under the conditions of temperature 25-35 DEG C, obtain fermentate, be processed further obtaining taro functional fermentation food Product.
The taro used in described step (1) is the fresh taro being cut into sheet or fragment.
The Monascus used in described step (2) is Monascus ruber, purple Monascus, feathering Monascus, peace carminum Any one or a few in aspergillus, chrysanthemum aspergillus or Monascus aurantiaaeus.
In described step (2), liquid submerged culture base is made up of the component of following percentage by weight: taro meal 1-5%, Maltose 1-10%, peptone 0.5-6.0%, ammonium chloride 0.01-0.5%, magnesium sulfate 0.01-0.2%, dipotassium hydrogen phosphate 0.01- 0.2%, surplus is distilled water, and above-mentioned each component sum is 100%, and control pH is 6.0-6.8.
In described step (2), liquid state fermentation condition is: fermentation temperature 28 DEG C, bottling amount 50-100mL/500mL triangle Bottle, rotating speed 150 revs/min, fermentation time 96 hours.
The described lactic acid bacteria in step (3) is lactobacillus paracasei, Lactobacillus plantarum, Pediococcus pentosaceus, the bright string of goldbeater's skin Any one or a few in pearl bacterium, Lactobacillus brevis, Pediococcus acidilactici, Lactobacillus coryniformis or Lactococcus lactis subsp. lactis.
In described step (3), the liquid lactic acid bacteria culture medium that spreads cultivation is made up of the component of following percentage by weight: taro meal 1%, maltose 1%, peptone 1%, dusty yeast 0.4%, Sodium acetate trihydrate 0.5%, Triammonium citrate 0.2%, dipotassium hydrogen phosphate 0.2%, magnesium sulfate 0.02%, manganese sulfate 0.005%, Tween 80 0.1%, surplus is distilled water, and above-mentioned each component sum is 100%, Control pH is 6.8-7.2.
In described step (3), liquid anaerobic fermentation conditions is: fermentation temperature 37 DEG C, bottling amount 50-100 mL/250 mL Triangular flask, 24 hours anaerobic fermentation time.
Slant medium, fluid nutrient medium and taro fermentation medium of using in described step (1), (2), (3) are equal Through 121 DEG C of sterilizing 10-60min.
The described fermentate in step (4) to constant weight, obtains taro merit through infrared, freezing or heat drying after pulverizing Can property fermented food.
What described preparation method prepared has regulation blood sugar, blood fat and improves gastrointestinal function and raising The functional fermented food of taro of immunity of organisms effect.
Compared with prior art, the invention has the beneficial effects as follows:
The functional fermented food of taro that the present invention provides, it has the plurality of health care functions of taro, red colouring agent for food, also used as a Chinese medicine and lactic acid bacteria concurrently, not only There is the reducing blood lipid of red colouring agent for food, also used as a Chinese medicine, the effect such as hypotensive, hypoglycemic and lactic acid bacteria improve gastrointestinal function, control endotoxin and raising machine Body immunity etc. act on, and also have raising immunity of organisms, the lowering blood pressure and blood fat of taro and prevent and treat the merits such as cardiovascular and cerebrovascular diseases Effect.Monascus bacterial strain and lactic acid bacteria strains are inoculated in taro solid medium and carry out mixed fungus fermentation by the present invention, the taro obtained Functional fermented food taste health more rich, to people has more health-care effect, more can meet many Man's Demands.
Below in conjunction with example, more specific description present invention.The preparation method of the present invention, it is not limited to real below Example, has any type of change to the preparation method of the present invention, falls within protection scope of the present invention.
Detailed description of the invention
Embodiment 1
The preparation method of the functional fermented food of a kind of taro, is prepared from by following steps:
(1) taro fermentation medium:
Taro is cleaned, every 100 kg taros are added ammonium chloride 0.02 kg, maltose 0.5 kg, magnesium sulfate 0.05 kg, phosphorus Acid hydrogen dipotassium 0.01 kg, water 100 L;
(2) Monascus liquid strain:
Monascus bacterial strain, after wort agar slant medium activation culture 5 days, accesses liquid submerged culture base and carries out liquid Fermented and cultured prepares Monascus liquid strain;
(3) lactic acid bacteria liquid strain:
Lactic acid bacteria strains, after MRS agar slant culture-medium anaerobism activation culture 1 day, accesses the liquid lactic acid bacteria culture medium that spreads cultivation and enters Row liquid anaerobic fermentation cultivates preparation lactic acid bacteria liquid strain;
(4) preparation of the functional fermented food of taro:
On the basis of the weight of the taro culture medium described in step (1), add the red colouring agent for food, also used as a Chinese medicine described in step (2) by weight percentage Lactic acid bacteria liquid strain 10% described in bacterium liquid strain 10%, step (3), fermented and cultured 7 days under the conditions of temperature 30 DEG C, i.e. Can produce and obtain the functional fermented food of taro.
The taro used in described step (1) is the fresh taro being cut into sheet.
The Monascus used in described step (2) is purple Monascus (CICC 40937).
In described step (2), liquid Shake flask medium is made up of the component of following percentage by weight: taro meal 5%, wheat Bud sugar 2%, peptone 4%, ammonium chloride 0.02%, magnesium sulfate 0.02%, dipotassium hydrogen phosphate 0.01%, surplus is distilled water, above-mentioned Each component sum is 100%, and controlling pH is 6.0.
In described step (2), liquid conditions of flask fermentation is: fermentation temperature 28 DEG C, bottling amount 100mL/500mL triangle Bottle, rotating speed 150 revs/min, fermentation time 96 hours.
The described lactic acid bacteria in step (3) is lactobacillus paracasei (CICC 20241).
In described step (3), the liquid lactic acid bacteria culture medium that spreads cultivation is made up of the component of following percentage by weight: taro meal 1%, maltose 1%, peptone 1%, dusty yeast 0.4%, Sodium acetate trihydrate 0.5%, Triammonium citrate 0.2%, dipotassium hydrogen phosphate 0.2%, magnesium sulfate 0.02%, manganese sulfate 0.005%, Tween 80 0.1%, surplus is distilled water, and above-mentioned each component sum is 100%, Controlling pH is 6.8.
In described step (3), liquid anaerobic fermentation conditions is: fermentation temperature 37 DEG C, and 100 mL/250 mL tri-are measured in bottling Angle bottle, 24 hours anaerobic fermentation time.
Described slant medium, fluid nutrient medium and taro fermentation medium is through 121 DEG C of sterilizing 30 min.
Described solid state fermentation thing, through infra-red drying to constant weight, obtains after pulverizing and has regulation blood sugar, blood fat and improvement The red colouring agent for food, also used as a Chinese medicine taro functional food of the effect such as gastrointestinal function.This functional fermented food outward appearance is red, has distinctive Ferment fragrance, containing Lovastatin 1.68 mg/g, monascorubin 6.54 mg/g.This functional fermented food has significantly regulation Blood sugar, blood fat and improve the effect such as gastrointestinal function, it is also possible to for producing the guarantor with auxiliary hyperglycemic and effect for reducing blood fat Health food.
Embodiment 2
The preparation method of the functional fermented food of a kind of taro, is prepared from by following steps:
(1) taro fermentation medium:
Taro is cleaned, every 100 kg taros are added ammonium chloride 0.02 kg, maltose 0.5 kg, magnesium sulfate 0.05 kg, phosphorus Acid hydrogen dipotassium 0.01 kg, water 100 L;
(2) Monascus liquid strain:
Monascus bacterial strain, after wort agar slant medium activation culture 5 days, accesses liquid submerged culture base and carries out liquid Fermented and cultured prepares Monascus liquid strain;
(3) lactic acid bacteria liquid strain:
Lactic acid bacteria strains, after MRS agar slant culture-medium anaerobism activation culture 1 day, accesses the liquid lactic acid bacteria culture medium that spreads cultivation and enters Row liquid anaerobic fermentation cultivates preparation lactic acid bacteria liquid strain;
(4) preparation of the functional fermented food of taro:
On the basis of the weight of the taro culture medium described in step (1), add the red colouring agent for food, also used as a Chinese medicine described in step (2) by weight percentage Lactic acid bacteria liquid strain 5% described in bacterium liquid strain 10%, step (3), fermented and cultured 5 days under the conditions of temperature 30 DEG C, i.e. Can produce and obtain the functional fermented food of taro.
The taro used in described step (1) is the fresh taro being cut into sheet.
The Monascus used in described step (2) is purple Monascus (CICC 40937).
In described step (2), liquid Shake flask medium is made up of the component of following percentage by weight: taro meal 5%, wheat Bud sugar 2%, peptone 5%, ammonium chloride 0.02%, magnesium sulfate 0.02%, dipotassium hydrogen phosphate 0.01%, surplus is distilled water, above-mentioned Each component sum is 100%, and controlling pH is 6.8.
In described step (2), liquid conditions of flask fermentation is: fermentation temperature 28 DEG C, bottling amount 100mL/500mL triangle Bottle, rotating speed 150 revs/min, fermentation time 96 hours.
The described lactic acid bacteria in step (3) is Leuconostoc mesenteroides (CICC 20054).
In described step (3), the liquid lactic acid bacteria culture medium that spreads cultivation is made up of the component of following percentage by weight: taro meal 1%, maltose 1%, peptone 1%, dusty yeast 0.4%, Sodium acetate trihydrate 0.5%, Triammonium citrate 0.2%, dipotassium hydrogen phosphate 0.2%, magnesium sulfate 0.02%, manganese sulfate 0.005%, Tween 80 0.1%, surplus is distilled water, and above-mentioned each component sum is 100%, Controlling pH is 7.2.
In described step (3), liquid anaerobic fermentation conditions is: fermentation temperature 37 DEG C, and 100 mL/250 mL tri-are measured in bottling Angle bottle, 24 hours anaerobic fermentation time.
Described slant medium, fluid nutrient medium and taro fermentation medium is through 121 DEG C of sterilizing 30 min.
Described solid state fermentation thing, through infra-red drying to constant weight, obtains after pulverizing and has regulation blood sugar, blood fat and improvement The red colouring agent for food, also used as a Chinese medicine taro functional food of the effect such as gastrointestinal function.This functional fermented food outward appearance is red, has distinctive Ferment fragrance, containing Lovastatin 1.45 mg/g, monascorubin 5.66 mg/g.This functional fermented food has significantly regulation Blood sugar, blood fat and improve the effect such as gastrointestinal function, it is also possible to for producing the guarantor with auxiliary hyperglycemic and effect for reducing blood fat Health food.
Embodiment 3
The preparation method of the functional fermented food of a kind of taro, is prepared from by following steps:
(1) taro fermentation medium:
Taro is cleaned, every 100 kg taros are added ammonium chloride 0.02 kg, maltose 0.5 kg, magnesium sulfate 0.05 kg, phosphorus Acid hydrogen dipotassium 0.01 kg, water 100 L;
(2) Monascus liquid strain:
Monascus bacterial strain, after wort agar slant medium activation culture 5 days, accesses liquid submerged culture base and carries out liquid Fermented and cultured prepares Monascus liquid strain;
(3) lactic acid bacteria liquid strain:
Lactic acid bacteria strains, after MRS agar slant culture-medium anaerobism activation culture 1 day, accesses the liquid lactic acid bacteria culture medium that spreads cultivation and enters Row liquid anaerobic fermentation cultivates preparation lactic acid bacteria liquid strain;
(4) preparation of the functional fermented food of taro:
On the basis of the weight of the taro culture medium described in step (1), add the red colouring agent for food, also used as a Chinese medicine described in step (2) by weight percentage Lactic acid bacteria liquid strain 5% described in bacterium liquid strain 10%, step (3), fermented and cultured 5 days under the conditions of temperature 30 DEG C, i.e. Can produce and obtain the functional fermented food of taro.
The taro used in described step (1) is the fresh taro being cut into sheet.
The Monascus used in described step (2) is purple Monascus (CICC 40937) and feathering Monascus (CICC 40710).
In described step (2), liquid Shake flask medium is made up of the component of following percentage by weight: taro meal 5%, wheat Bud sugar 2%, peptone 6%, ammonium chloride 0.02%, magnesium sulfate 0.02%, dipotassium hydrogen phosphate 0.01%, surplus is distilled water, above-mentioned Each component sum is 100%, and controlling pH is 6.0.
In described step (2), liquid conditions of flask fermentation is: fermentation temperature 28 DEG C, bottling amount 100mL/500mL triangle Bottle, rotating speed 150 revs/min, fermentation time 96 hours.
The described lactic acid bacteria in step (3) is Leuconostoc mesenteroides (CICC 20054) and lactobacillus paracasei (CICC 20241).
In described step (3), the liquid lactic acid bacteria culture medium that spreads cultivation is made up of the component of following percentage by weight: taro meal 1%, maltose 1%, peptone 1%, dusty yeast 0.4%, Sodium acetate trihydrate 0.5%, Triammonium citrate 0.2%, dipotassium hydrogen phosphate 0.2%, magnesium sulfate 0.02%, manganese sulfate 0.005%, Tween 80 0.1%, surplus is distilled water, and above-mentioned each component sum is 100%, Controlling pH is 7.2.
In described step (3), liquid anaerobic fermentation conditions is: fermentation temperature 37 DEG C, and 100 mL/250 mL tri-are measured in bottling Angle bottle, 24 hours anaerobic fermentation time.
Described slant medium, fluid nutrient medium and taro fermentation medium is through 121 DEG C of sterilizing 20 min.
Described solid state fermentation thing, through infra-red drying to constant weight, obtains after pulverizing and has regulation blood sugar, blood fat and improvement The red colouring agent for food, also used as a Chinese medicine taro functional food of the effect such as gastrointestinal function.This functional fermented food outward appearance is red, has distinctive Ferment fragrance, containing Lovastatin 0.66 mg/g, monascorubin 8.6 mg/g.This functional fermented food has significantly regulation Blood sugar, blood fat and improve the effect such as gastrointestinal function, it is also possible to for producing the guarantor with auxiliary hyperglycemic and effect for reducing blood fat Health food.
Embodiment 4
The preparation method of the functional fermented food of a kind of taro, is prepared from by following steps:
(1) taro fermentation medium:
Taro is cleaned, every 100 kg taros are added ammonium chloride 0.02 kg, maltose 0.5 kg, magnesium sulfate 0.05 kg, phosphorus Acid hydrogen dipotassium 0.01 kg, water 100 L;
(2) Monascus liquid strain:
Monascus bacterial strain, after wort agar slant medium activation culture 5 days, accesses liquid submerged culture base and carries out liquid Fermented and cultured prepares Monascus liquid strain;
(3) lactic acid bacteria liquid strain:
Lactic acid bacteria strains, after MRS agar slant culture-medium anaerobism activation culture 1 day, accesses the liquid lactic acid bacteria culture medium that spreads cultivation and enters Row liquid anaerobic fermentation cultivates preparation lactic acid bacteria liquid strain;
(4) preparation of the functional fermented food of taro:
On the basis of the weight of the taro culture medium described in step (1), add the red colouring agent for food, also used as a Chinese medicine described in step (2) by weight percentage Lactic acid bacteria liquid strain 5% described in bacterium liquid strain 10%, step (3), fermented and cultured 5 days under the conditions of temperature 30 DEG C, i.e. Can produce and obtain the functional fermented food of taro.
The taro used in described step (1) is the fresh taro being cut into bulk.
The Monascus used in described step (2) is feathering Monascus (CICC 40710).
In described step (2), liquid Shake flask medium is made up of the component of following percentage by weight: taro meal 5%, wheat Bud sugar 2%, peptone 6%, ammonium chloride 0.02%, magnesium sulfate 0.02%, dipotassium hydrogen phosphate 0.01%, surplus is distilled water, above-mentioned Each component sum is 100%, and controlling pH is 6.8.
In described step (2), liquid conditions of flask fermentation is: fermentation temperature 28 DEG C, bottling amount 100mL/500mL triangle Bottle, rotating speed 150 revs/min, fermentation time 96 hours.
The described lactic acid bacteria in step (3) is Leuconostoc mesenteroides (CICC 20054) and Pediococcus pentosaceus (ATCC 33316).
In described step (3), the liquid lactic acid bacteria culture medium that spreads cultivation is made up of the component of following percentage by weight: taro meal 1%, maltose 1%, peptone 1%, dusty yeast 0.4%, Sodium acetate trihydrate 0.5%, Triammonium citrate 0.2%, dipotassium hydrogen phosphate 0.2%, magnesium sulfate 0.02%, manganese sulfate 0.005%, Tween 80 0.1%, surplus is distilled water, and above-mentioned each component sum is 100%, Controlling pH is 7.2.
In described step (3), liquid anaerobic fermentation conditions is: fermentation temperature 37 DEG C, and 100 mL/250 mL tri-are measured in bottling Angle bottle, 24 hours anaerobic fermentation time.
Described slant medium, fluid nutrient medium and taro fermentation medium is through 121 DEG C of sterilizing 30 min.
Described solid state fermentation thing, through infra-red drying to constant weight, obtains after pulverizing and has regulation blood sugar, blood fat and improvement The red colouring agent for food, also used as a Chinese medicine taro functional food of the effect such as gastrointestinal function.This functional fermented food outward appearance is red, has distinctive Ferment fragrance, containing Lovastatin 2.3 mg/g, monascorubin 0.90 mg/g.This functional fermented food has significantly regulation Blood sugar, blood fat and improve the effect such as gastrointestinal function, it is also possible to for producing the guarantor with auxiliary hyperglycemic and effect for reducing blood fat Health food.
The foregoing is only presently preferred embodiments of the present invention, all impartial changes done according to scope of the present invention patent with Modify, all should belong to the covering scope of the present invention.

Claims (10)

1. the preparation method of the functional fermented food of taro, it is characterised in that be prepared from by following steps:
(1) taro fermentation medium: add ammonium chloride 0.01-0.4 kg, maltose 0.01-1.0 kg in every 100 kg taros, Magnesium sulfate 0.001-0.1 kg, dipotassium hydrogen phosphate 0.001-0.1 kg, water 50-150 L;
(2) Monascus liquid strain: Monascus bacterial strain, after wort agar slant medium activation culture 3-6 days, accesses liquid Body Shake flask medium carries out liquid state fermentation cultivation and prepares Monascus liquid strain;
(3) lactic acid bacteria liquid strain: lactic acid bacteria strains, after MRS agar slant culture-medium anaerobism activation culture 1-2 days, accesses liquid Body lactic acid bacteria spread cultivation culture medium carry out liquid anaerobic fermentation cultivate preparation lactic acid bacteria liquid strain;
(4) preparation of the functional fermented food of taro: on the basis of the weight of the taro culture medium described in step (1), by weight Monascus liquid strain 1-20% described in percentages addition step (2), the lactic acid bacteria liquid strain 1-described in step (3) 10%, fermented and cultured 5-8 days under the conditions of temperature 25-35 DEG C, obtain fermentate, be processed further obtaining taro functional fermentation food Product.
The preparation method of the functional fermented food of a kind of taro the most according to claim 1, it is characterised in that described step Suddenly the Monascus used in (2) be Monascus ruber, purple Monascus, feathering Monascus, Monascus anka bacterium, chrysanthemum aspergillus or Any one or a few in Monascus aurantiaaeus.
The preparation method of the functional fermented food of a kind of taro the most according to claim 1, it is characterised in that described step Suddenly in (2), liquid submerged culture base is made up of the component of following percentage by weight: taro meal 1-5%, maltose 1-10%, albumen Peptone 0.5-6.0%, ammonium chloride 0.01-0.5%, magnesium sulfate 0.01-0.2%, dipotassium hydrogen phosphate 0.01-0.2%, surplus is distillation Water, above-mentioned each component sum is 100%, and control pH is 6.0-6.8.
The preparation method of the functional fermented food of a kind of taro the most according to claim 1, it is characterised in that described step Suddenly in (2), liquid state fermentation condition is: fermentation temperature 28 DEG C, bottling amount 50-100mL/500mL triangular flask, rotating speed 150 revs/min, Fermentation time 96 hours.
The preparation method of the functional fermented food of a kind of taro the most according to claim 1, it is characterised in that described step Suddenly the lactic acid bacteria in (3) is lactobacillus paracasei, Lactobacillus plantarum, Pediococcus pentosaceus, Leuconostoc mesenteroides, Lactobacillus brevis, breast Any one or a few in acid sheet coccus, Lactobacillus coryniformis or Lactococcus lactis subsp. lactis.
The preparation method of the functional fermented food of a kind of taro the most according to claim 1, it is characterised in that described In step (3), the liquid lactic acid bacteria culture medium that spreads cultivation is made up of the component of following percentage by weight: taro meal 1%, maltose 1%, egg White peptone 1%, dusty yeast 0.4%, Sodium acetate trihydrate 0.5%, Triammonium citrate 0.2%, dipotassium hydrogen phosphate 0.2%, magnesium sulfate 0.02%, Manganese sulfate 0.005%, Tween 80 0.1%, surplus is distilled water, and above-mentioned each component sum is 100%, and control pH is 6.8-7.2.
The preparation method of the functional fermented food of a kind of taro the most according to claim 1, it is characterised in that described In step (3), liquid anaerobic fermentation conditions is: fermentation temperature 37 DEG C, and bottling amount 50-100 mL/250 mL triangular flask, anaerobism is sent out 24 hours ferment time.
The preparation method of the functional fermented food of a kind of taro the most according to claim 1, it is characterised in that described step Suddenly the slant medium, fluid nutrient medium and the taro fermentation medium that use in (1), (2), (3) are all through 121 DEG C of sterilizing 10- 60min。
The preparation method of the functional fermented food of a kind of taro the most according to claim 1, it is characterised in that described step Suddenly the fermentate in (4) is through infrared, freezing or heat drying to constant weight, obtains the functional fermented food of taro after pulverizing.
10. prepared the having of preparation method as claimed in claim 1 regulates blood sugar, blood fat, improves intestines and stomach merit The functional fermented food of taro of the multi-efficiency of immunity of organisms and can be improved.
CN201610271495.0A 2016-04-28 2016-04-28 Preparation method of taro functional fermented food Pending CN105918961A (en)

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