CN105918461A - Low-calorific-value yogurt containing phascolosoma esculenta polysaccharide and preparation method thereof - Google Patents

Low-calorific-value yogurt containing phascolosoma esculenta polysaccharide and preparation method thereof Download PDF

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Publication number
CN105918461A
CN105918461A CN201610247333.3A CN201610247333A CN105918461A CN 105918461 A CN105918461 A CN 105918461A CN 201610247333 A CN201610247333 A CN 201610247333A CN 105918461 A CN105918461 A CN 105918461A
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China
Prior art keywords
sipunculoidea
mixed
polysaccharide
parts
fermented
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CN201610247333.3A
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Chinese (zh)
Inventor
刘连亮
曹锦轩
楼乔明
苗亮
王艳
陈炯
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Ningbo University
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Ningbo University
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Priority to CN201610247333.3A priority Critical patent/CN105918461A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00Milk preparations; Milk powder or milk powder preparations
    • A23C9/12Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/13Fermented milk preparations; Treatment using microorganisms or enzymes using additives
    • A23C9/1307Milk products or derivatives; Fruit or vegetable juices; Sugars, sugar alcohols, sweeteners; Oligosaccharides; Organic acids or salts thereof or acidifying agents; Flavours, dyes or pigments; Inert or aerosol gases; Carbonation methods

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  • Life Sciences & Earth Sciences (AREA)
  • Microbiology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Dairy Products (AREA)

Abstract

The present invention provides low-calorific-value yogurt containing phascolosoma esculenta polysaccharides. The yogurt consists of the following components in parts by weight: 0.5-20 parts of phascolosoma esculenta polysaccharides, 1-5 parts of a mixed bacterium fermentation agent, 5-10 parts of oligosaccharides, 2-5 parts of xylitol, 0.2-0.6 part of a mixed stabilizer, and the balance being raw milk to a total quantitative amount of 100 parts. The present invention also discloses a preparation method of the above-mentioned fermented yogurt containing the phascolosoma esculenta polysaccharides. The preparation method comprises the following steps: (1) raw material pre-treating and blending, (2) preheating and homogenizing, (3) sterilizing and cooling, (4) inoculating, (5) fermenting, and (6) cold-storing and after-ripening. The product is soft and delicate in taste, refreshing and smooth, moderately sweet and sour, and coordinative in flavor, has special flavor and texture of the phascolosoma esculenta and yogurt, is even in color and luster, has no stratification, is rich in nutrients of the phascolosoma esculenta polysaccharides, and prebiotics, dietary fibers and other components beneficial for the growth of probiotics, can maintain digestive health and prevent cardiovascular disease, diabetes and other diseases, complies with the product positioning purpose of "functionalization and nutrification", and can meet the needs of modern consumers.

Description

A kind of low heat value Yoghourt containing Sipunculoidea polysaccharide and preparation method
Technical field
The present invention relates to a kind of Sipunculoidea polysaccharide low heat value Yoghourt and preparation method, belong to food processing technology field.
Background technology
Phascolosoma (Phascolosoma esculenta), it is commonly called as Hai Ding, Sipunculoidea etc., belong to Genus Golfingia (Sipuncula), leather bag Sipunculida (Phascolosoma tidea), leather bag Sipunculoidea mesh (Phascoloso-maliformes), for China's indigenous species.China's multi-section pharmacopeia have recorded the edible of Phascolosoma and medical value, " Cordyceps " being referred to as in ocean.Phascolosoma meat flavour is delicious, nutritious, several amino acids, trace element and various active material rich in needed by human body, can effectively regulate the various function of human body, there is the effects such as significant slow down aging, resisting fatigue, antioxidation and promotion mouse milk secretion, be one of food of liking of the China coast people with Phascolosoma for " sipunculid worm jelly " that raw material makes.
The research of Phascolosoma nutritional labeling finds, is up to 66% containing protein in its dry sample, and it is mainly composed of collagen protein.Existing Chinese patent literature CN101381411B provides a kind of Sipunculid collagen protein and preparation method thereof, there is reduced immunogenicity, gelation, water absorption, retentiveness and emulsibility, high dispersion and low cohesiveness, especially wound healing is had obvious facilitation;Patent documentation CN103483462A provides preparation method and the antisepsis activity thereof of a kind of Phascolosoma polysaccharide;CN101210261 provides the preparation method of a kind of sipunculid polypeptide, and the Sipunculoidea protein polypeptide obtained shows good antioxidation and prevents the athletic injury caused that moves, and shows sport nutrition activity.
On the basis of it is an object of the invention to provide a kind of Sipunculoidea polysaccharide uniqueness health value, Sipunculoidea polysaccharide is organically combined with Yoghourt, obtain the one soft exquisiteness of mouthfeel, tasty and refreshing lubrication, sour-sweet moderate, flavor coordination, have Sipunculoidea and the distinctive local flavor of Yoghourt concurrently.The sweeting agent that additionally product of the present invention uses is oligosaccharide, so that product is not only rich in whole nutrients and the active component of bamboo sprout, and rich in contributing to the probiotic bacteria prebiotics at people's proliferation in vivo.
Summary of the invention
It is an object of the invention to provide Sipunculoidea polysaccharide low heat value Yoghourt and preparation method.
The present invention is achieved through the following technical solutions: (1) pretreatment of raw material and allotment: by Sipunculoidea polysaccharide 0.5-20 part, oligosaccharide 5-10 part, xylitol 2-5 part, mixed stabilizer 0.2-0.6 part, by consumption mix homogeneously, pour in the raw milk of mix homogeneously, enter back into colloid mill and fully mix formation mixing breast;
(2) preheating and homogenizing: the mixing breast that step (1) is sufficiently stirred for mixing is preheating to 45-55 DEG C, then enters and carries out homogenizing in homogenizer, and homogenization pressure is 20-25MPa;
(3) sterilizing and cooling: continue 5-10min at 85-90 DEG C and carry out sterilizing, be cooled to 40-50 DEG C;
(4) inoculation: the fermented by mixed bacterium agent prepared, fermentation temperature 40-45 DEG C, joins the mixing Ruzhong after cooling by determined inoculum concentration fermented by mixed bacterium agent, quantitatively to 100 parts, stirs;
(5) fermentation: postvaccinal mixing breast moves to ferment at constant temperature room, ferments 3-4 hour under the conditions of 40-45 DEG C, and mixed curdling is solid, when pH value reaches 4.2-4.6, stops fermentation, and cools down immediately;
(6) cold preservation, after-ripening: cold preservation after-ripening in 12-24 hour under conditions of 1-6 DEG C;
Detailed description of the invention
Embodiment 1
(1) pretreatment of raw material and allotment: by Sipunculoidea polysaccharide 5 parts, oligosaccharide 5 parts, xylitol 2 parts, mixed stabilizer 0.2 part, by consumption mix homogeneously, pour in the raw milk of mix homogeneously, enters back into colloid mill and fully mixes formation mixing breast;
(2) preheating and homogenizing: the mixing breast that step (1) is sufficiently stirred for mixing is preheating to 45 DEG C, then enters and carries out homogenizing in homogenizer, and homogenization pressure is 25MPa;
(3) sterilizing and cooling: continue 5min at 85 DEG C and carry out sterilizing, be cooled to 40 DEG C;
(4) inoculation: the fermented by mixed bacterium agent prepared, fermentation temperature 40 DEG C, joins the mixing Ruzhong after cooling by determined inoculum concentration fermented by mixed bacterium agent, stirs;
(5) fermentation: postvaccinal mixing breast moves to ferment at constant temperature room, ferments 4 hours under the conditions of 40 DEG C, and mixed curdling is solid, when pH value reaches 4.2, stops fermentation, and cools down immediately;
(6) cold preservation, after-ripening: cold preservation after-ripening in 24 hours under conditions of 1 DEG C;
Embodiment 2
(1) pretreatment of raw material and allotment: by Sipunculoidea polysaccharide 10 parts, oligosaccharide 8 parts, mixed stabilizer 0.4 part, by consumption mix homogeneously, pour in the raw milk of mix homogeneously, enters back into colloid mill and fully mixes formation mixing breast;
(2) preheating and homogenizing: the mixing breast that step (1) is sufficiently stirred for mixing is preheating to 50 DEG C, then enters and carries out homogenizing in homogenizer, and homogenization pressure is 20MPa;
(3) sterilizing and cooling: continue 8min at 90 DEG C and carry out sterilizing, be cooled to 45 DEG C;
(4) inoculation: the fermented by mixed bacterium agent prepared, fermentation temperature 45 DEG C, joins the mixing Ruzhong after cooling by determined inoculum concentration fermented by mixed bacterium agent, stirs;
(5) fermentation: postvaccinal mixing breast moves to ferment at constant temperature room, ferments 3.5 hours under the conditions of 45 DEG C, and mixed curdling is solid, when pH value reaches 4.4, stops fermentation, and cools down immediately;
(6) cold preservation, after-ripening: cold preservation after-ripening in 18 hours under conditions of 4 DEG C;
Embodiment 3
(1) pretreatment of raw material and allotment: by Sipunculoidea polysaccharide 20 parts, oligosaccharide 10 parts, mixed stabilizer 0.6 part, by consumption mix homogeneously, pour in the raw milk of mix homogeneously, enters back into colloid mill and fully mixes formation mixing breast;
(2) preheating and homogenizing: the mixing breast that step (1) is sufficiently stirred for mixing is preheating to 55 DEG C, then enters and carries out homogenizing in homogenizer, and homogenization pressure is 23MPa;
(3) sterilizing and cooling: continue 10min at 88 DEG C and carry out sterilizing, be cooled to 50 DEG C;
(4) inoculation: the fermented by mixed bacterium agent prepared, fermentation temperature 50 DEG C, joins the mixing Ruzhong after cooling by determined inoculum concentration fermented by mixed bacterium agent, stirs;
(5) fermentation: postvaccinal mixing breast moves to ferment at constant temperature room, ferments 3 hours under the conditions of 50 DEG C, and mixed curdling is solid, when pH value reaches 4.6, stops fermentation, and cools down immediately;
(6) cold preservation, after-ripening: cold preservation after-ripening in 12 hours under conditions of 6 DEG C;
Finally, in addition it is also necessary to be only several specific embodiments of the present invention it is noted that listed above.It is clear that the invention is not restricted to above example, it is also possible to there are many deformation.All deformation that those of ordinary skill in the art can directly derive from present disclosure or associate, are all considered as protection scope of the present invention.

Claims (7)

1. the low heat value Yoghourt containing Sipunculoidea polysaccharide, it is characterised in that this Yoghourt is composed of the following components in parts by weight: Sipunculoidea polysaccharide 0.5-20 part, fermented by mixed bacterium agent 1-5 part, oligosaccharide 5-10 part, xylitol 2-5 part, mixed stabilizer 0.2-0.6 part, raw material milk is quantitatively to 100 parts.
The low heat value Yoghourt of Sipunculoidea polysaccharide the most according to claim 1, it is characterised in that described Sipunculoidea polysaccharide: Sipunculoidea (Phascolosoma or Sipunculus nudus) obtains Sipunculoidea homogenate through homogenate;With Sipunculoidea homogenate as raw material, papain enzymolysis 3-5h, ethanol gradient precipitates, it is thus achieved that Sipunculoidea polysaccharide.
Fermented by mixed bacterium agent the most according to claim 1, it is characterised in that Lactobacillus bulgaricus, streptococcus thermophilus, plant lactobacillus, bacillus bifidus one or more arbitrarily assemble with any mass ratio.
Fermented by mixed bacterium agent the most according to claim 1, it is characterised in that prepare the plant lactobacillus of described leaven and bacillus bifidus is through the bile tolerance of mutagenic and breeding and the Lactobacillus plantarum of the Gastric juice digestion of resistance to intestinal and bacillus bifidus.
Oligosaccharide the most according to claim 1, it is characterised in that one or more in Oligomeric maltose, oligofructose, dextrinosan and stachyose arbitrarily assemble with any mass ratio.
Mixed stabilizer the most according to claim 1, it is characterized in that hydroxypropyl PASELLI EASYGEL, pectin, casein sodium phosphate one or more arbitrarily assemble with any mass ratio.
7. a preparation method for the low heat value Yoghourt of Sipunculoidea polysaccharide as claimed in claim 1, comprises the following steps:
(1) pretreatment of raw material and allotment: Sipunculoidea polysaccharide, oligosaccharide, xylitol, mixed stabilizer are mixed homogeneously by consumption, pour in the raw milk of mix homogeneously, enters back into colloid mill and fully mixes formation mixing breast;
(2) preheating and homogenizing: the mixing breast that step (1) is sufficiently stirred for mixing is preheating to 45-55 DEG C, then enters and carries out homogenizing in homogenizer, and homogenization pressure is 20-25MPa;
(3) sterilizing and cooling: continue 5-10min at 85-90 DEG C and carry out sterilizing, be cooled to 40-50 DEG C;
(4) inoculation: the fermented by mixed bacterium agent prepared, fermentation temperature 40-45 DEG C, joins the mixing Ruzhong after cooling by determined inoculum concentration fermented by mixed bacterium agent, stirs;
(5) fermentation: postvaccinal mixing breast moves to ferment at constant temperature room, ferments 3-4 hour under the conditions of 40-45 DEG C, and mixed curdling is solid, when pH value reaches 4.2-4.6, stops fermentation, and cools down immediately;
(6) cold preservation, after-ripening: cold preservation after-ripening in 12-24 hour under conditions of 1-6 DEG C.
CN201610247333.3A 2016-04-08 2016-04-08 Low-calorific-value yogurt containing phascolosoma esculenta polysaccharide and preparation method thereof Pending CN105918461A (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1748714A (en) * 2004-09-14 2006-03-22 李妍妍 Method for preparing biological active substance of siphon-worm and its products
CN102153666A (en) * 2011-02-22 2011-08-17 中国人民解放军海军医学研究所 Sipunculidae polysaccharide and preparation method thereof and application thereof to anti-fatigue function
CN102177965A (en) * 2011-06-08 2011-09-14 山东省农业管理干部学院 Low-heat-value yoghurt jelly containing superfine full rose powder and preparation method thereof
CN102488003A (en) * 2011-11-28 2012-06-13 上海师范大学 Yogurt containing stevioside, and production method thereof
CN103330204A (en) * 2013-07-03 2013-10-02 广西壮族自治区海洋研究所 Sipunculus nudus polysaccharide oral liquid

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1748714A (en) * 2004-09-14 2006-03-22 李妍妍 Method for preparing biological active substance of siphon-worm and its products
CN102153666A (en) * 2011-02-22 2011-08-17 中国人民解放军海军医学研究所 Sipunculidae polysaccharide and preparation method thereof and application thereof to anti-fatigue function
CN102177965A (en) * 2011-06-08 2011-09-14 山东省农业管理干部学院 Low-heat-value yoghurt jelly containing superfine full rose powder and preparation method thereof
CN102488003A (en) * 2011-11-28 2012-06-13 上海师范大学 Yogurt containing stevioside, and production method thereof
CN103330204A (en) * 2013-07-03 2013-10-02 广西壮族自治区海洋研究所 Sipunculus nudus polysaccharide oral liquid

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
张桂和: "方格星虫多糖分离纯化及性质鉴定", 《食品与生物技术学报》 *

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Application publication date: 20160907

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