CN105918117A - Propagation method of tetraploid winter jujube - Google Patents
Propagation method of tetraploid winter jujube Download PDFInfo
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- CN105918117A CN105918117A CN201610367712.6A CN201610367712A CN105918117A CN 105918117 A CN105918117 A CN 105918117A CN 201610367712 A CN201610367712 A CN 201610367712A CN 105918117 A CN105918117 A CN 105918117A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
- A01H1/08—Methods for producing changes in chromosome number
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/30—Grafting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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Abstract
The invention discloses a propagation method of a tetraploid winter jujube. The method is characterized in that the systematic adjustment is carried out during the steps of explant inoculating, primary culture, subculture, rooting culture, transition seedling hardening, greenhouse potting, field nursery planting, ploidy determination of induced plant, grafting, and determining of fruit quality, so as to obtain excellent tetraploid winter jujube nursery stocks. With the adoption of the method, the excellent tetraploid winter jujube nursery stocks can be obtained; the method is vegetative propagation method and cannot be influenced by season, so that the winter jujub can be purified and optimized more conveniently and quickly; cultured winter jujube trees are generally outstanding in performances, and high in disease resistance and stress resistance; the nursery stocks are strong, and short in joints, and have developed root systems; the fruits have the characteristics of being large in size, and free of kernels. Therefore, the method is higher in economic benefit.
Description
Technical field
The present invention relates to the cultural method of plant, be specifically related to the mating system of a kind of tetraploid Fructus Jujubae.
Background technology
At present, the Production of fruit of China is in a brand-new competitive environment, excellent fruit and high-quality and efficient producer
Formula is the key obtaining good benefit.The characteristic fruit originated in for China, strengthens research dynamics, improves nutrient quality, strengthens
Competitiveness, it has also become one of key problem in China's Fruit industry.China is area of origin and the cultivation centers of jujube tree, plantation money
Source is the abundantest.But current diploid Fructus Jujubae exists that fruit is less than normal, pit is big, peel is thin and storage property is poor, subjects to pest and disease damage etc.
Shortcoming, the yield and peasant household covets, use HORMONE TREATMENT to increase fruit, greatly reduce the fine quality of Fructus Jujubae.According to tune
Looking into, the high-quality date rate of Fructus Jujubae is less than 10% in the market, and persticide residue exceeds standard more than 95%, and the factor of merit is low, pesticide residues
Amount height, there is no the high-grade Fructus Jujubae reaching export standard.These problems greatly limit sustainable development and the city of Zhanhua winter jujube
Field competitiveness, it has also become the considerable hurdle that restriction Zhanhua winter jujube industry develops in a healthy way.
It addition, polyploidization is one of major way of plant evolution, polyploid plant many performances nutrition organs and genitals
The huge property of official, has high miscellaneous and property, stronger adaptability and resistance.Fruit tree typically has in heredity height heterozygosis, excellent
The features such as the existence of good polyploid variety and generally energy asexual propagation, are more suitable for induction and the cultivation of polyploid.Up to the present,
At pears, Fructus Pruni, Fructus Persicae, Fructus Rubi, chestnut, gooseberry, Fructus Mali pumilae, Fructus Musae, Fructus Vitis viniferae, Fructus Fragariae Ananssae, Pericarpium Citri tangerinae, mandarin orange, Fructus Ananadis comosi, Fructus actinidiae chinensis, Semen Juglandis, Fructus Pruni pseudocerasi etc.
Successfully induce polyploid plant on fruit tree, greatly enrich the Fruit Tree Germplasm Resources of countries in the world.Luring of polyploidization plant
Lead the process being not only related to induced gene sudden change, with greater need for combining distinctive cultivation, because reasonably cultivation is same
Time be also one of the important way improved of tree characteristics.And at present jujube tree only has Golden jujube and obtain four times by Colchicine
The report of body plant, the tetraploid plant for Fructus Jujubae is induced and is cultivated the research combined and had no relevant report before the application
Road.
Summary of the invention
In order to solve above the problems of the prior art, the side of breeding proposing a kind of tetraploid Fructus Jujubae of the invention
Method, uses the method can obtain the tetraploid Fructus Jujubae plant of high-quality, belongs to asexual propagation, not by seasonal effect, make Fructus Jujubae kind
Purification select excellent convenient and quick, it is excellent that the Fructus Jujubae tree bred generally has character, disease-resistant, strong stress resistance, nursery stock is healthy and strong,
Internode is short, well developed root system, and fruit has the features such as big, seedless.
The technical scheme that the present invention takes is as follows:
A kind of mating system of tetraploid Fructus Jujubae, its feature step that is to plant is as follows:
(1) outer implant inoculation: the Fructus Jujubae head branch just sprouted at season of growth clip or Secondary Branch terminal bud 2cm, does with aseptic water washing
Only, then disinfect in alcohol on super-clean bench, then sterilize respectively with sodium hypochlorite and mercuric chloride, finally do with aseptic water washing
Only；
(2) initial culture: material is placed under anatomical lens and strips the growing point of 0.3-0.5mm with dissecting needle and be inoculated into MS+
In the culture medium of BA2.0mg/L+IBA0.4, do induction with Colchicine liquid and process, when the bud length sent is to 3cm, by joint
The section of being cut into, as successive propagation material；Described Colchicine sets 0.05%, 0.1%, 0.3%, 0.5%4 concentration, processes
Time sets 12h, 24h, 48h, 72h, 96h 5 and processes the time.
(3) successive transfer culture: the bud tip newly extracted out by initial culture is cut into the stem section of 1cm, stem section retains a more than joint, switching
On MS+BA1.5+IBA0.5 subculture medium, continuous differentiation and proliferation that the follow-up bud tip cultivating formation is transferred repeatedly；
(4) root culture: the bud tip on subculture medium is cut into the stem section of 1cm band 1-2 joint, receives suitable root media
1/2 MS+NAA1/L+IBA0.5/L+LH200/L
Upper cultivation, after test tube Seedling stem section root length reaches more than 2cm, de-bottle is transplanted；
(5) transition seedling exercising: after rooting of vitro seedling cultivates 5 weeks, top grows the healthy and strong branch and leaf of more than 2cm, is moved on to by test tube Seedling
Carry out soilless substrate transition seedling exercising at greenhouse lucifuge, transplant initial stage relative humidity and be maintained at about 80%, temperature 28 30 DEG C.
(6) greenhouse pot culture: Fructus Jujubae Seedling, after 20 days, is moved in nutritive cube or little biscuiting basin in greenhouse by seedling exercising, and substrate is with specially
The Nutrition Soil (perlite Vermiculitum peat composed of rotten mosses garden mould ratio of weight and number is 11 0.5 0.5) of door preparation.
(7) nursery, land for growing field crops: after 30 days to be grown, moves into nursery, land for growing field crops from nutritive cube or little biscuiting basin by Fructus Jujubae Seedling；
(8) Ploidy Identification of plant is induced: leaf index, internode carries out morphologic qualification as index, utilizes routine pressure
Sheet method identify induction plant chromosome number, utilize fluorescence microscopy guard cell's number of chloroplast, gas roll size and
Density, utilizes the DNA content of flow cytomery induction plant cell.
(9) grafting: the tetraploid plantlet after identifying is grafted onto on adult Fructus Jujubae tree, carries out the basin of polyploid plant
Plant resistant proof, field resistance test, select the strain that resistance level is high, character is excellent.
(10) mensuration of fruit quality: the strain that antagonism level is high, character is excellent carries out the mensuration of fruit quality, fruit
Hardness utilizes GY-1 type disk durometer to measure, and the soluble solids content of fruit utilizes ATAGO digital refractometer PRl to measure,
Fruit Vitamin C content uses difference analysis spectrophotometer method to measure, the size of fruit and gravimetry, the presence or absence of pit,
Thus select excellent tetraploid Fructus Jujubae nursery stock.
Sterilisation step in above step (1) is particularly as follows: with 70% alcohol disinfecting 5s on super-clean bench, with the hypochlorous acid of 5%
Sodium and 0.1% mercuric chloride sterilize respectively 8min.
Receiving 8-10d on root media in above step (4), the axillalry bud of test tube Seedling stem section starts to sprout, stem section simultaneously
Base portion produces white projection, bears 35 little Bletilla striata (Thunb.ex A.Murray)Rchb.f. after 12 days, and after 30 40 days, root length reaches more than 2cm.
In above step (5) soilless substrate be the mixture of perlite, Vermiculitum, specially soilless substrate be perlite and trematodiasis
Stone mass ratio is 1:3 mixture.
The invention have the benefit that the application aseptically, the tissue of Fructus Jujubae plant or individual cells from planting
Take off on object, cultivate in being placed on the glass container by factors such as Artificial Control illumination, temperature, humidity and nutrient substance, make it
Survival or development, finally develop into a complete Fructus Jujubae plant, carries out under the environmental condition that indoors artificial controls, and belongs to
In asexual propagation, Fast-propagation can be realized and produce；Artificial Control, not by seasonal effect；Save soil；Screening property is strong, can be numerous
Grow virus-free Seedling；Variety purification selects excellent convenient.The Zhanhua winter jujube nursery stock produced by this method can keep its parent excellent
Optimum shape, and detoxification, disease-resistant, resistance is obviously enhanced；Nursery stock itself is healthy and strong, internode is short, well developed root system；Enter in advance and contain fruit
Phase, result quality better.
Utilize Colchicine-induced Tetraploid Zhanhua winter jujube, it is expected to while keeping Fructus Jujubae fruit fine quality, make fruit become
Being twice left and right, individual tree volume increase at least 50%, benefit is significantly increased, and fundamentally solution peasant household uses HORMONE TREATMENT to increase
Big fruit causes the impact of Zhanhua winter jujube quality serious degradation；The tetraploid Fructus Jujubae that induction produces doubles due to hereditary material,
Storage, disease resistance etc. have the change of merit, and storage time is longer, and during storage, moisture loss is less；Disease resistance is bright
Aobvious enhancing, can be greatly decreased the usage amount of pesticide, such that it is able to improvement Soil structure, reduces environmental pollution, protects natural enemy, dimension
Protect diversity of organism and ecological balance, it is achieved the benign cycle of agricultural production, there are higher ecological benefits.
Detailed description of the invention
The following examples are that the present invention is described in further detail.
Embodiment:
1. project research place
According to this project characteristic, test is at many place development.Efficiently foundation, adaptability and the resistance of Fructus Jujubae essence point cultivating system
Test is carried out at Zhanhua County Fructus Jujubae institute；The Ploidy Identification of plant, fruit quality measure at Shandong University's Life Science College
Cell engineering test chamber is carried out；The field selection-breeding of Fructus Jujubae nursery stock is numerous the Chinese new and high technology nursery stock of Zhanhua County Fructus Jujubae institute subordinate
Educate field to carry out.
2. Project-developing scale
The different phase of project research, scale is ascending, and the Resistance Identification stage is ascending, and the scale of every kind of material is 1000
About square metre, to development phase popularization.After terminating to project, build up breeding Demonstration Base 100 mu, reach to produce 300000 per year
The production capacity of strain tetraploid Fructus Jujubae new varieties nursery stock.
3. Project-developing content
(1) with Colchicine ex vivo treatment Zhanhua winter jujube stem apex, Fructus Jujubae multiploid induction technical system is set up.
(2) utilize the surfaces such as shortened internodes, leaf index diminish that induction plant is carried out preliminary times of sex determination, carefully
Born of the same parents learn detection and chromosome counting identifies plant ploidy further.On this basis, flow cytometer is utilized to measure the DNA of cell
Content, the final ploidy judging induction plant.
(3) by acceleration of growth in tetraploid autumn branches to adult jujube tree and the most solid, tetraploid plant is entered simultaneously
Row morphological characteristic and the research of growth promoter.
(4) Fructus Jujubae of observation tetraploid Zhanhua winter jujube really character and change collection period, select elite plant；To the Fructus Jujubae gathered
Fruit carries out the research of the aspect such as fruit quality, storage period, selects the Zhanhua winter jujube plant that fruit is big, of fine quality.
(5) on the basis of the analysis that excellent tetraploid plant is carried out Comprehensive Traits, breeding nursery stock model field is set up,
The breeding carrying out nursery stock product is promoted, to play a role on producing as early as possible.
4. research method
(1) outer implant inoculation: the Fructus Jujubae head branch just sprouted at clip at the beginning of by the end of April 5 months or Secondary Branch terminal bud 2cm, uses aseptic water washing
Totally, then with 70% alcohol disinfecting 5s on super-clean bench, sodium hypochlorite with 5% and the mercuric chloride of 0.1% are sterilized 8min respectively,
Finally clean with aseptic water washing.
(2) initial culture: material is placed under anatomical lens and strips the growing point of 0.3-0.5mm with dissecting needle and be inoculated into MS+
In the culture medium of BA2.0mg/L+IBA0.4, doing induction with Colchicine liquid and process, Colchicine sets 0.05%, and 0.1%,
0.3%, 0.5%4 concentration, the process time sets 12h, 24h, 48h, 72h, 96h 5 and processes the time, and bud length to be sent is extremely
During 3cm, by the joint section of being cut into, as successive propagation material；
(3) successive transfer culture: the bud tip newly extracted out by initial culture is cut into the stem section of 1cm, stem section retains a more than joint, is transferred to MS
On+BA1.5+IBA0.5 subculture medium, continuous differentiation and proliferation that the follow-up bud tip cultivating formation is transferred repeatedly；
(4) root culture: the bud tip on subculture medium is cut into the stem section of 1cm band 1-2 joint, receives suitable root media
Cultivating 8-10d on (1/2 MS+NAA1/L+IBA0.5/L+LH200/L), the axillalry bud of test tube Seedling stem section starts to sprout
Dynamic, stem section base portion produces white projection simultaneously, bears 35 little Bletilla striata (Thunb.ex A.Murray)Rchb.f. after 12 days, and after 30 40 days, root length reaches more than 2cm
After, de-bottle is transplanted；
(5) transition seedling exercising: after rooting of vitro seedling cultivates 5 weeks, top grows the healthy and strong branch and leaf of more than 2cm, is moved on to by test tube Seedling
Carrying out soilless substrate transition seedling exercising at greenhouse lucifuge, soilless substrate is perlite and Vermiculitum mass ratio is 1:3 mixture, at the beginning of transplanting
Phase relative humidity is maintained at about 80%, temperature 28 30 DEG C.
(6) greenhouse pot culture: seedling exercising is after 20 days, and Fructus Jujubae Seedling shoot and leaf growth is good, in greenhouse, Fructus Jujubae Seedling is moved into nutritive cube or little
In biscuiting basin, substrate perlite Vermiculitum peat composed of rotten mosses garden mould is 11 0.5 0.5 preparations by ratio of weight and the number of copies.
(7) nursery, land for growing field crops: after 30 days to be grown, and the growth of Fructus Jujubae Seedling is vigorous, it is moved into from nutritive cube or little biscuiting basin
Nursery, land for growing field crops；
(8) Ploidy Identification of plant is induced: utilize conventional compression to identify the chromosome number of induction plant, utilize fluidic cell
The DNA content of instrument detection induction plant cell.Recording leaf index is 1.85, average 3.7 centimetres of internode.Utilize conventional compression
Identifying the chromosome number of induction plant, chromosome number is 48.Utilizing the amount measured by flow cytometer is relative value, profit
It is measured as tetraploid with flow cytometer.
(9) grafting: the tetraploid plantlet after identifying is grafted onto on the Fructus Jujubae tree that big Tanaka grows up, and carries out polyploid and plants
The resistance of strain (the most cold-resistant, drought resisting, waterlogging-resistant, Resistant, resistance etc.) viewing test, selects that resistance level is high, character is excellent
Strain.
(10) mensuration of fruit quality: the strain that antagonism level is high, character is excellent carries out the mensuration of fruit quality, fruit
Hardness utilizes GY-1 type disk durometer to measure, and the soluble solids content of fruit utilizes ATAGO digital refractometer PRl to measure,
Fruit Vitamin C content uses difference analysis spectrophotometer method to measure, the size of fruit and gravimetry, the presence or absence of pit,
Thus select excellent tetraploid Fructus Jujubae nursery stock.
Generally referring to of recording is designated as: record fruit average hardness 8.7kg/cm2, the soluble solids content of fruit utilizes
ATAGO digital refractometer PRl measures, and soluble solid is 33%.Fruit Vitamin C content uses difference analysis spectrophotometric
Meter method measures, vitamin C 308 100g, mean fruit weight 21.09g, maximum single fruit weight 57.5g, and fruit shape index is 0.918,
Pit is little.But above index not for the present invention screening concrete point value index, about above index it is also contemplated that.
5. implementation result
Setting up the technical system of high-efficiency in-vitro induction four times of plant of Zhanhua winter jujube, induction frequency is not less than 6%, it is thus achieved that tetraploid is stained with
Changing Fructus Jujubae plant 400 strain, the highest being connected to is grown up on jujube tree, accelerates to produce and fruit development.Then tetraploid of reentrying knot Fructus Jujubae
Plant 300 strain, therefrom selects fruit and increases more than 20%, and keep the polyploid plant 20-30 strain of fine quality, select
Plant 10 strain that fruit keeping quality or disease resistance strengthen.
By cultivating tetraploid plant, its fruit volume will significantly increase, and individual tree will increase production 50%, and benefit has and significantly carries
Height, and its storage property and resistance will be obviously enhanced.
Claims (8)
1. the mating system of a tetraploid Fructus Jujubae, it is characterised in that step of planting is as follows:
(1) outer implant inoculation: the Fructus Jujubae head branch just sprouted at season of growth clip or Secondary Branch terminal bud 2cm, does with aseptic water washing
Only, then disinfect in alcohol on super-clean bench, then sterilize respectively with sodium hypochlorite and mercuric chloride, finally do with aseptic water washing
Only；
(2) initial culture: material is placed under anatomical lens and strips the growing point of 0.3-0.5mm with dissecting needle and be inoculated into MS+
In the culture medium of BA2.0mg/L+IBA0.4, do induction with Colchicine liquid and process, when the bud length sent is to 3cm, by joint
The section of being cut into, as successive propagation material；
(3) successive transfer culture: the bud tip newly extracted out by initial culture is cut into the stem section of 1cm, stem section retains a more than joint, is transferred to MS
On+BA1.5+IBA0.5 subculture medium, continuous differentiation and proliferation that the follow-up bud tip cultivating formation is transferred repeatedly；
(4) root culture: the bud tip on subculture medium is cut into the stem section of 1cm band 1-2 joint, receives and trains on root media
Supporting, after test tube Seedling stem section root length reaches more than 2cm, de-bottle is transplanted；
(5) transition seedling exercising: after rooting of vitro seedling cultivates 5 weeks, top grows the healthy and strong branch and leaf of more than 2cm, is moved on to by test tube Seedling
Carry out soilless substrate transition seedling exercising at greenhouse lucifuge, transplant initial stage relative humidity and be maintained at 78%-82%, temperature 28 30 DEG C；
(6) greenhouse pot culture: Fructus Jujubae Seedling, after 20 days, is moved in nutritive cube or little biscuiting basin in greenhouse by seedling exercising, substrate perlite,
The Nutrition Soil of Vermiculitum, the peat composed of rotten mosses, garden mould preparation；
(7) nursery, land for growing field crops: after 30 days to be grown, moves into nursery, land for growing field crops from nutritive cube or little biscuiting basin by Fructus Jujubae Seedling；
(8) Ploidy Identification of plant is induced: leaf index, internode carries out morphologic qualification as index, utilizes routine pressure
Sheet method identify induction plant chromosome number, utilize fluorescence microscopy guard cell's number of chloroplast, gas roll size and
Density, utilizes the DNA content of flow cytomery induction plant cell；
(9) grafting: the tetraploid plantlet after identifying is grafted onto on adult Fructus Jujubae tree, carries out the potted plant anti-of polyploid plant
Property test, field resistance test, select the strain that resistance level is high, character is excellent；
(10) mensuration of fruit quality: the strain that antagonism level is high, character is excellent carries out the mensuration of fruit quality, the hardness of fruit
Utilizing GY-1 type disk durometer to measure, the soluble solids content of fruit utilizes ATAGO digital refractometer PRl to measure, fruit
Vitamin C content uses difference analysis spectrophotometer method to measure, the size of fruit and gravimetry, the presence or absence of pit, thus
Select excellent tetraploid Fructus Jujubae nursery stock.
The mating system of a kind of tetraploid Fructus Jujubae the most as claimed in claim 1, it is characterised in that sterilisation step tool in step (1)
Body is: with 70% alcohol disinfecting 5s on super-clean bench, and sodium hypochlorite with 5% and the mercuric chloride of 0.1% are sterilized 8min respectively.
The mating system of a kind of tetraploid Fructus Jujubae the most as claimed in claim 1, it is characterised in that: step (4) is received and takes root
8-10d in culture medium, the axillalry bud of test tube Seedling stem section starts to sprout, and stem section base portion produces white projection simultaneously, bears 3 after 12 days
Article 5, little Bletilla striata (Thunb.ex A.Murray)Rchb.f., after 30 40 days, root length reaches more than 2cm.
4. the mating system of a kind of tetraploid Fructus Jujubae as described in claim 1 or 3, it is characterised in that: described root culture
Base is 1/2 MS+NAA1/L+IBA0.5/L+LH200/L culture medium.
The mating system of a kind of tetraploid Fructus Jujubae the most as claimed in claim 1, it is characterised in that: step (6) mesostroma
Nutrition Soil by the ratio of weight and number preparation that perlite, Vermiculitum, the peat composed of rotten mosses, garden mould are 1:1:0.5:0.5.
The mating system of a kind of tetraploid Fructus Jujubae the most as claimed in claim 1, it is characterised in that: soilless substrate in step (5)
For perlite, the mixture of Vermiculitum.
The mating system of a kind of tetraploid Fructus Jujubae the most as claimed in claim 6, it is characterised in that: described soilless substrate is precious
Zhu Yan and Vermiculitum mass ratio are 1:3 mixture.
The mating system of a kind of tetraploid Fructus Jujubae the most as claimed in claim 1, it is characterised in that: described Colchicine sets
0.05%, 0.1%, 0.3%, 0.5%4 concentration, the process time sets 12h, 24h, 48h, 72h, 96h 5 and processes the time.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107047315A (en) * | 2017-06-01 | 2017-08-18 | 滨州市沾化区冬枣实业总公司 | It is a kind of that the method that chimera obtains pure tetraploid winter jujube plant is purified by limb section callus adventitious shoot regeneration |
| CN107047314A (en) * | 2017-06-01 | 2017-08-18 | 滨州市沾化区冬枣实业总公司 | A kind of method that pure tetraploid winter jujube plant is obtained by blade callus induction and adventitious bud |
| CN107135942A (en) * | 2017-06-04 | 2017-09-08 | 滨州市沾化区冬枣实业总公司 | A kind of breeding method of triploid winter jujube |
| CN107432195A (en) * | 2017-05-22 | 2017-12-05 | 滨州市沾化区冬枣研究所 | A kind of winter jujube engrafting and cultivating method |
| CN111557176A (en) * | 2020-04-30 | 2020-08-21 | 深圳市国艺园林建设有限公司 | Tissue culture and rapid propagation method for desert rose |
| CN113330972A (en) * | 2021-05-27 | 2021-09-03 | 河北农业大学 | Method for cultivating large seedlings of jujube rootstock in light-matrix container |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1672498A (en) * | 2005-04-04 | 2005-09-28 | 山东大学 | Method of establishing polyploiding system for Dongzao date |
| CN104082146A (en) * | 2014-07-14 | 2014-10-08 | 北京林业大学 | Method for inducing polyploid through wild jujube adventitious buds |
-
2016
- 2016-05-30 CN CN201610367712.6A patent/CN105918117A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1672498A (en) * | 2005-04-04 | 2005-09-28 | 山东大学 | Method of establishing polyploiding system for Dongzao date |
| CN104082146A (en) * | 2014-07-14 | 2014-10-08 | 北京林业大学 | Method for inducing polyploid through wild jujube adventitious buds |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107432195A (en) * | 2017-05-22 | 2017-12-05 | 滨州市沾化区冬枣研究所 | A kind of winter jujube engrafting and cultivating method |
| CN107047315A (en) * | 2017-06-01 | 2017-08-18 | 滨州市沾化区冬枣实业总公司 | It is a kind of that the method that chimera obtains pure tetraploid winter jujube plant is purified by limb section callus adventitious shoot regeneration |
| CN107047314A (en) * | 2017-06-01 | 2017-08-18 | 滨州市沾化区冬枣实业总公司 | A kind of method that pure tetraploid winter jujube plant is obtained by blade callus induction and adventitious bud |
| CN107135942A (en) * | 2017-06-04 | 2017-09-08 | 滨州市沾化区冬枣实业总公司 | A kind of breeding method of triploid winter jujube |
| CN111557176A (en) * | 2020-04-30 | 2020-08-21 | 深圳市国艺园林建设有限公司 | Tissue culture and rapid propagation method for desert rose |
| CN113330972A (en) * | 2021-05-27 | 2021-09-03 | 河北农业大学 | Method for cultivating large seedlings of jujube rootstock in light-matrix container |
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