CN105902761A - Application of flos sophorae powder in preparing drugs for treating hyperglycemia and diabetes - Google Patents
Application of flos sophorae powder in preparing drugs for treating hyperglycemia and diabetes Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/489—Sophora, e.g. necklacepod or mamani
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/484—Glycyrrhiza (licorice)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/488—Pueraria (kudzu)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/738—Rosa (rose)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/75—Rutaceae (Rue family)
- A61K36/752—Citrus, e.g. lime, orange or lemon
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/894—Dioscoreaceae (Yam family)
- A61K36/8945—Dioscorea, e.g. yam, Chinese yam or water yam
Abstract
The invention relates to the application of flos sophorae powder in preparing drugs for treating hyperglycemia and diabetes to effectively solve the problem of drug use for treating hyperglycemia and diabetes. Fresh flos sophorae is washed, dried in a vacuum environment, evenly mixed with earth-fried rhizoma dioscoreae, puerariae thomsonii radix, rose flower, pericarpium citri reticulatae and licorice root, and then smashed into fine powder, namely flos sophorae powder, and then packaging and sterilization are conducted. Raw materials are rich, the preparing method is simple, obtained drugs can be effectively used for treating hyperglycemia and diabetes, the application of flos sophorae powder in preparing drugs for treating hyperglycemia and diabetes is realized and is an innovation in drugs for treating hyperglycemia and diabetes, and economic and social benefits are remarkable.
Description
Technical field
The present invention relates to medicine, the precious application in preparation treatment hyperglycemia and diabetes medicament of a kind of Flos Sophorae.
Background technology
When blood glucose value is higher than being hyperglycemia normal range.Hyperglycemia is also usual high in " three high " described in everybody.Additionally " two is high " is hypertension and hyperlipemia respectively.Fasting glucose normal value is at below 6.1mmol/L, and the normal value of two hours blood glucoses is at below 7.8mmol/L after the meal, if above this scope, referred to as hyperglycemia.Under normal circumstances, by hormonal regulation and this two big regulation system of neuroregulation, human body can guarantee that the source of blood glucose keeps balance with outlet, make blood glucose maintain certain level.But at inherited genetic factors (such as Diabetes family history) with under the common effect of environmental factors (such as irrational meals, obesity etc.), two big regulatory functions get muddled, and arise that the rising of blood sugar level.
Diabetes are one group of metabolic diseases being characterized with hyperglycemia.Hyperglycemia is then owing to defect of insulin secretion or its biological agent are impaired, or both have concurrently and cause.Long-standing hyperglycemia during diabetes, causes various tissue, particularly eye, kidney, heart, blood vessel, neural chronic lesion, dysfunction, clinical manifestation polydipsia, polyuria, polyphagia and become thin, fatigue and weak, obesity.
Hyperglycemia and diabetes drastically influence the healthy of people, have treatment hyperglycemia and the multi-medicament of diabetes though existing, but for various reasons, its use and curative effect are the most unsatisfactory, and therefore, the medicine developing new treatment hyperglycemia and diabetes is imperative.
Summary of the invention
For above-mentioned situation, for overcoming the defect of prior art, the purpose of the present invention is just to provide the precious application in preparation treatment hyperglycemia and diabetes medicament of a kind of Flos Sophorae, can effectively solve the medicine for treatment problem of hyperglycemia and diabetes.
The technical scheme that the present invention solves is, the precious application in preparation treatment hyperglycemia and diabetes medicament of a kind of Flos Sophorae, this Flos Sophorae treasure is to take fresh Flos Sophorae, cleans, vacuum drying, then mix homogeneously with parching with earth RHIIZOMA DIOSCOREAE fr0m Henan of China, Pachyrhizua angulatus, Flos Rosae Rugosae, Pericarpium Citri Reticulatae and Radix Glycyrrhizae, be ground into fine powder, be Flos Sophorae precious (powder), packaging, sterilizing.
Abundant raw material of the present invention, preparation method is simple, is effective to treat hyperglycemia and diabetes, it is achieved the precious application in preparation treatment hyperglycemia and diabetes medicament of Flos Sophorae, is the innovation on treatment hyperglycemia and diabetes medicament, and economic and social benefit is notable.
Detailed description of the invention
Below in conjunction with concrete condition, the detailed description of the invention of the present invention is elaborated.
The present invention is in being embodied as, the precious application in preparation treatment hyperglycemia and diabetes medicament of a kind of Flos Sophorae, this Flos Sophorae treasure is to take fresh Flos Sophorae, clean, 60 DEG C of vacuum drying, then mix homogeneously with parching with earth RHIIZOMA DIOSCOREAE fr0m Henan of China, Pachyrhizua angulatus, Flos Rosae Rugosae, Pericarpium Citri Reticulatae and Radix Glycyrrhizae, were ground into the fine powder of 100 mesh sieves, it is Flos Sophorae precious (powder), packaging, sterilizing, fresh Flos Sophorae is 321 0.5 0.3 0.2 with the weight ratio of parching with earth RHIIZOMA DIOSCOREAE fr0m Henan of China, Pachyrhizua angulatus, Flos Rosae Rugosae, Pericarpium Citri Reticulatae and Radix Glycyrrhizae.
In said components, wherein Flos Sophorae is fabaceous flower and alabastrum, and fresh Flos Sophorae refers to the fresh Flos Sophorae gathered from Sophora japonica L., Flos Sophorae bitter in the mouth, and property is put down, nontoxic, has effect of heat clearing away, removing heat from blood, hemostasis, blood pressure lowering.Haematemesis, hematuria, bleeding hemorrhoids, wind heat conjunctival congestion, hypertension, hyperlipemia, the tuberculosis of cervical lymph nodes, sclerosis of blood vessels, stool band blood, diabetes, retinitis, psoriasis etc. there is significant curative effect；Can with anthelmintic, control pharyngitis.Flos Sophorae can strengthen the resistance of blood capillary, reduces vascular permeability, and fragility blood vessel can be made to recover elastic function, thus blood fat reducing and prevent sclerosis of blood vessels.
Parching with earth RHIIZOMA DIOSCOREAE fr0m Henan of China is RHIIZOMA DIOSCOREAE fr0m Henan of China east wall soil or humus flava usta (known technology), to strengthen effect of spleen reinforcing stomach invigorating.
Pachyrhizua angulatus: Pachyrhizua angulatus is pulse family, the mutation (Classification system: Pueraria lobata (Willd.) Ohwi var.thomsonii (Benth.) Vaniot der Maesen) of Pueraria Pueraria lobota, Chinese medicine name, cure mainly fever caused by exogenous pathogens, rigidity pain on the head and nape, measles from the beginning of, rash go out smooth, epidemic febrile disease is thirsty, quench one's thirst, have loose bowels, dysentery, hypertension, coronary heart disease.
Flos Rosae Rugosae hardship sweet, micro-, temperature.Return liver, spleen channel, promoting QI circulation for relieving depression, and blood, pain relieving.For stomachache due to hyperactive liver-QI attacking the stomach, lack of appetite vomiting and nausea, menoxenia, fall and flutter the pain of injury.
Pericarpium Citri Reticulatae bitter in the mouth, pungent, warm in nature, return lung, spleen channel, regulating qi-flowing for strengthening spleen, drying dampness to eliminate phlegm.
Radix Glycyrrhizae feeble QI, taste is sweet and special.Function cures mainly heat-clearing and toxic substances removing, expelling phlegm for arresting cough, gastral cavity abdomen etc..
The efficient combination of said components, support mutually, there is effect of blood sugar lowering, it is effective to treat hyperglycemia and diabetes, realize the precious application in preparation treatment hyperglycemia and diabetes medicament of Flos Sophorae, and achieve extraordinary Advantageous Effects through site test and application, relevant testing data is as follows:
1 experiment material
1.1 pharmaceutical agent
Flos Sophorae of the present invention is precious, and (Henan university of TCM laboratory provides, every bag of 10g, each 1-2 bag, three times a day)；Metformin hydrochloride, Shanghai Pharmaceutical's letter friendship pharmacy head factory produces；Adrenalin hydrochloride injection, Shanghai Hefeng Pharmaceutical Co., Ltd. produces；Alloxan, sigma company produces；Streptozotocin, sigma company produces；Normal saline, Zhengzhou Yonghe Pharmaceutical Co produces；Concentrated sulphuric acid, Luoyang City's chemical reagent factory produces；Citric acid (analytical pure), pharmaceuticals of Hubei Province glass station；Sodium citrate (analytical pure), chemical reagent wholesale department, Tianjin；Blood sugar kit, Zhejiang Dong Ou biological engineering company limited produces；Liver glycogen test kit, Nanjing is built up Bioengineering Research Institute and is produced；Glycated serum protein (GSP) measures test kit, and Nanjing is built up Bioengineering Research Institute first point and produced.
1.2 laboratory animal
Mice: Kunming kind, SPF level, male, Henan Province's medical experiment animal center provide, the quality certification number: 008373.
1.3 experimental apparatus
UV-2000 ultraviolet-uisible spectrophotometer: UNICO(Shanghai) Instruments Co., Ltd. produces；Thermostat water bath, light instrument plant of Beijing produces；Centrifuge, Beijing Medical Instruments repair shop produces；FA (N)/JA (N) series electronic balance, Shanghai Min Qiao precision instrument company limited produces；Adjustable pipette, Shanghai Lei Bo Analytical Instrument Co., Ltd produces.
1.4 test solution preparations
The preparation of citrate buffer: weighing 1292.4mg citric acid respectively and 1132.3mg sodium citrate is made into 100ml solution, it is 4.2 citrate buffers that this solution is PH, for guaranteeing that its accuracy can be allocated with PH instrumental correction.(streptozotocin is the most sufficiently stable, and relatively big to vascular stimulation, can alleviate these negative effects with buffer streptozotocin): prepared before use.
The preparation of 10% formalin fixative: 36% formalin 10ml, is made into 100ml with distilled water.
2 experimental techniques
2.1 packets and administration
2.1.1 epinephrine is caused the impact of mice hyperglycemia model
Take male mice 50, body weight 20～22g, it is uniformly divided into 5 groups at random, gavages Flos Sophorae treasure's suspension (16g/kg, 8g/kg, 0.4g/ml, 0.2g/ml of large and small dosage respectively, 0.4ml/10g), metformin suspension (0.208g/kg, 0.052mg/ml, 0.4ml/10g), model group and blank group gavage the physiological salt liquid (, 0.4ml/10g) of same volume.Every morning is administered once, afternoon feed, continuous 9 days.12h after fasting, every Mus rapid lumbar injection epinephrine 240ug/kg in addition to blank group, blank group injection same volume normal saline, after to epinephrine, 30min, 60min eye socket immediately takes blood, separates serum, for surveying blood glucose.Then after putting to death mice, weighing liver, system homogenate, for surveying liver glycogen.
2.1.2 alloxan is caused the impact of mice hyperglycemia model
Take male mice 50, body weight 20～22g, it is uniformly divided into 5 groups at random, gavages Flos Sophorae treasure's suspension (16g/kg, 8g/kg, 0.4g/ml, 0.2g/ml of large and small dosage respectively, 0.4ml/10g), metformin suspension (0.208g/kg, 0.052mg/ml, 0.4ml/10g), model group and blank group gavage the physiological salt liquid (, 0.4ml/10g) of same volume.Every morning is administered once, afternoon feed, continuous 10 days.90min after 12h perfusion after fasting in the 7th day, the alloxan physiological salt liquid of every tail vein injection 80mg/kg (0.05ml/10g) newly preparation, 72h after injection in addition to blank group, mouse orbit takes blood, separates serum, for surveying blood glucose.Then after putting to death mice, weighing liver, system homogenate, for surveying liver glycogen.
2.1.3 streptozotocin is caused the impact of diabetes mice model
Take male mice, normal raising 3 days, after fasting 12h, tail vein injection streptozotocin 80 (mg/kg, 0.02ml/10g), after the 11st day fasting 12h of injection, fasting blood sugar is surveyed in afterbody blood sampling, choose blood glucose value > 11.1mmol/L there is obvious polydipsia, polyphagia, the mice of polyuria symptom 40, 4 groups it are divided at random by blood glucose value, gavage big respectively, in, low dose of Flos Sophorae treasure suspension (16g/kg, 8g/kg, 0.4g/ml, 0.2g/ml, 0.4ml/10g), metformin suspension (0.208g/kg, 0.052mg/ml, 0.4ml/10g) the normal saline (model group) of same volume.Separately take and be only used as blank group with batch mice 10, gavage same volume normal saline.Every morning is administered once (feedwater), afternoon feed, continuous 30 days.Blood glucose value is surveyed in taking blood to the 10th, 20,30 days afterbodys of feed.Within 30th day, after fasting 12h last is administered 1h, mouse orbit takes blood, separates serum, for surveying blood glucose.Then after putting to death mice, weighing liver, system homogenate, for surveying liver glycogen；Taking pancreas, 10% formalin solution is fixed, and makees pathological section, for om observation pancreatic tissues situation.
2.2 index determining
2.2.1 serum blood sugar level
Mouse orbit takes blood, separates serum, illustrates that OD value is surveyed in operation, by test kit formula scales blood glucose value by test kit.
Agent formulations and concentration
Operational approach
Result calculates: glucose content (nmol/L)=Ar/As × 5.55 (100mg/dL)
Unit conversion: mg/dl × 0.0555=mmol/L
2.2.2 hepatic glycogen content
After eye socket takes blood, putting to death mice, take liver, precision weighs liver about 50mg, is illustrated in each pipe OD value of survey at 620nm by liver glycogen test kit, by test kit formula scales liver glycogen value.
Sample pretreatment
Sampling: after taking the rinsing of fresh liver normal saline, filter paper blots, and weighs
Hydrolysis: by sample weight (mg): alkali liquor volume (ul)=1:3, adds in test tube together, and boiling water bath boils 20 minutes, and flowing water cools down.
Glycogen hydrolyzed solution is further prepared into glycogen detection liquid:
Body development detection liquid is 5%, and the amount adding distilled water is: liver weight × 20-liver weight × 4=liver weight × 16
Operation table
Mixing is placed in boiling water boils 5 minutes, and in 620nm wavelength after cooling, 1cm optical path, blank tube returns to zero, surveys each pipe OD value
Computing formula: extension rate × 10 ÷ 1.11 before glycogen content (mg/g tissue)=mensuration pipe OD value/standard pipe OD value × standard pipe content (0.01mg) × test sample
3 experimental results
3.1 cause mouse blood sugar level and the impact of hepatic glycogen content to epinephrine
By blood sugar kit description and hepatic glycogen test kit description, measure blood sugar level and the hepatic glycogen content of each group of mice respectively, the results are shown in Table 1.
Table 1 Flos Sophorae is precious causes mice hyperglycemia model blood sugar level and the impact of hepatic glycogen content to epinephrine
* with model group than P ＜ 0.05, * * with model group than P ＜ 0.01
Can be seen that from upper table, with blank group ratio, model group significantly raises (P 0.01) 30 minutes and 60 minutes blood sugar levels, and liver glycogen level significantly reduces (P 0.01), illustrates to make the success of epinephrine hyperglycemia model.With model group ratio, large and small dosage Flos Sophorae treasure group and metformin group can significantly reduce 30 minutes blood sugar levels (P 0.01), can significantly raise liver glycogen level (P 0.01), metformin group can reduce by 60 minutes blood sugar levels (P 0.01) by significance, and heavy dose of Flos Sophorae treasure's group can obviously reduce 60 minutes blood sugar levels (P 0.05).
3.2 cause mouse blood sugar level and the impact of hepatic glycogen content to alloxan
Operate by blood sugar kit description and hepatic glycogen test kit description, measure blood sugar level and the hepatic glycogen content of each group of mice respectively, the results are shown in Table 2.
Table 2 Flos Sophorae is precious causes mice hyperglycemia model blood sugar level and the impact of liver glycogen content to alloxan
* with model group than P ＜ 0.05, * * with model group than P ＜ 0.01
Can be seen that from upper table, with blank group ratio, model group blood sugar level significantly raises (P 0.01), and liver glycogen level significantly reduces (P 0.01), illustrates to make the success of mice alloxan hyperglycemia model.With model group ratio, large and small dosage Flos Sophorae treasure group and metformin group can significantly reduce mice alloxan hyperglycemia model sugar level (P 0.01), heavy dose of Flos Sophorae treasure's group can significantly raise liver glycogen level (P 0.01), and low dose of Flos Sophorae treasure group and metformin group can significantly raised liver glycogen levels (P 0.05).
3.3 cause diabetes mice model blood glucose and the impact of pancreatic tissues form to streptozotocin
Operate by blood sugar kit description and hepatic glycogen test kit description, measure blood sugar level and the hepatic glycogen content of each group of mice respectively, the results are shown in Table 3.Om observation pancreatic tissues is cut into slices, and each group pancreas different pathological change is shown in Table 4.
The precious impact on streptozotocin cause diabetes mice model blood sugar level of table 3 Flos Sophorae (mmol/l)
* with model group than P ＜ 0.05, * * with model group than P ＜ 0.01
As can be seen from Table 3, with blank group ratio, being administered the 30th day from starting to be administered into, model group blood sugar level significantly raises (P 0.01), liver glycogen level significantly reduces (P 0.01), illustrates to make the success of mice streptozotocin diabetes model.Each administration group starts no significant difference between blood glucose, illustrates that packet is uniformly；Being administered the 10th day, each administration group all reduces than model group, but no significant difference；It is administered the 20th day, organizes mouse blood sugar level all significantly reduce (P 0.01) with model group ratio, metformin group and heavy dose of Flos Sophorae treasure；Being administered the 30th day, with model group ratio, large and small dosage Flos Sophorae treasure organizes and metformin group all can significantly reduce model mice blood sugar level (P 0.01), and low dose of Flos Sophorae treasure organizes and substantially reduces blood sugar level (P 0.05)；Large and small dosage Flos Sophorae treasure group and metformin group all can notable elevation model Mouse Liver glycogen levels (P 0.01).
The precious impact that streptozotocin is caused diabetes mice model pancreatic tissues of table 4 Flos Sophorae
" " islet cells endochylema enriches, and atrophy, edema and vacuolar degeneration does not occurs, the most normally；"+" islet cells presents atrophy phenomenon, and without edema and vacuolar degeneration；" ++ " islet cells major part occurs that atrophy, cell minority edema occur and without vacuolar degeneration；There is atrophy in " +++ " islet cells, edema and the obvious person of vacuolar degeneration occurs simultaneously.
Can be seen that from upper table, check through Ridit, with blank group ratio, model group pancreas pathological change is notable (P 0.01), illustrates to make the success of diabetes mice model.With model group ratio, heavy dose of Flos Sophorae treasure organizes the pathological change (P 0.01) that all can significantly alleviate pancreas.Each group mice pancreatic tissues om observation is visible: blank group Mice Islet Cells endochylema is abundant, cell space is big, islets of langerhans volume is the biggest；It is intensive that islet cells endochylema in model group mouse islets significantly reduces, cell space reduces, cell seems, islet cells part occurs that cavity becomes；Islet cells part cell cytosol in metformin group mouse islets is abundanter, and part cell cytosol significantly reduces, and cell space is obviously reduced, and core is intensive；The most cells that heavy dose of Flos Sophorae treasure organizes mice pancreatic tissues is restored, and endochylema enriches, and respective cells is edematous state, and around atrophy occurs in small part cell；The islet cells major part that low dose of Flos Sophorae treasure organizes in mice pancreatic tissues presents atrophing state, and cell volume reduces, and core is intensive, and certain atrophy occurs in a few cell, but has part endochylema.
Clinical data:
According to WHO2 patients with type Ⅰ DM standard, " new Chinese medicine guideline of clinical investigations " formulates the standard of this research: diabetes standard: have classical symptom (polydipsia, polyphagia, polyuria, weight loss, skin pruritus etc.), fasting glucose > 7.0mmol/L or post-prandial glycemia >=11.1mmol/L；Not there is classical symptom, fasting glucose > 7.0mmol/L or post-prandial glycemia >=11.1mmol/L, carbohydrate tolerance test 2 hours blood glucose 11.1mmol/L.QI and blood blood stasis standard: primary symptom: ache of the spinal column, tired unable, or edema, anorexia or wrist are swollen, dim complexion, and colour of the tongue is purple dark or has petechia, ecchymosis.Secondary disease: big loose stool, frequent micturition or frequent urination at night, body of the tongue is light red, has indentation, white and thin fur, squamous and dry skin or numb limbs and tense tendons, thready pulse or the most puckery, and whole blood, plasma viscosity raise.Primary symptom two adds in time disease any two, and it is dark or have petechia, ecchymosis to rise senior middle school's any one with hemorheology detection whole blood, plasma viscosity to possess purplish tongue, the most diagnosable.
Criterion of therapeutical effect.Effective: blood glucose is down to normal or blood glucose decline substantially > 3.0mmol/L；Patients symptomatic disappears substantially, and hemorheology index has clear improvement.Effective: blood glucose declines > 1.0mmol/L；Patients symptomatic is improved, and hemorheology index makes moderate progress；Invalid: patient blood glucose, symptom, sign are without improving, and indices is not up to effective standard, even deteriorates.
Blood stasis due to qi deficiency patients with type Ⅰ DM 42 example, male 26 examples, female 16 example, age 32-63 year, diabetic duration 0.5-2.Flos Sophorae is precious: 1-2 bag every time, and boiled water is taken after mixing it with water, every bag of 10g, three times a day.It within one month, it is a course for the treatment of.During application Flos Sophorae treasure, keep on a diet.The course for the treatment of terminates: 42 example blood stasis due to qi deficiency diabeticss, 6 examples are effective, 32 examples are effective, and 4 examples are invalid.Total effective rate 90.5%.
4 conclusions
Above-mentioned experiment fully proves, Flos Sophorae treasure can significantly reduce the blood sugar content of the hyperglycemia mice caused by epinephrine；Significantly reduce alloxan diabetes model mice and the hyperglycemia of streptozotocin-diabetes model mice.Flos Sophorae treasure can also promote that blood glucose is converted into glycogen, significantly improves epinephrine and the content of alloxan diabetes model mice liver glycogen.Flos Sophorae treasure can also improve streptozotocin-diabetes Mice Islet Cells form and function, plays good hypoglycemic activity.Heavy dose of group is better than small dose group.That is along with the increase of dosage, blood sugar reducing function is the most obvious, and the increase of liver glycogen content is the most notable, presents a certain amount effect relationship.The blood sugar reducing function mechanism of result prompting Flos Sophorae treasure is probably it and antiadrenergic drug is decomposed hepatic glycogen；Improve islet cells form and function, the beta Cell of islet promoting animal injury recovers, and is effective to preparation treatment hyperglycemia and the medicine of diabetes, it is achieved the precious application in preparation treatment hyperglycemia and diabetes medicament of Flos Sophorae, having the using value of reality, economic and social benefit is notable.
Claims (1)
1. the precious application in preparation treatment hyperglycemia and diabetes medicament of Flos Sophorae, this Flos Sophorae treasure is, take fresh Flos Sophorae, clean, 60 DEG C of vacuum drying, then mix homogeneously with parching with earth RHIIZOMA DIOSCOREAE fr0m Henan of China, Pachyrhizua angulatus, Flos Rosae Rugosae, Pericarpium Citri Reticulatae and Radix Glycyrrhizae, it is ground into the fine powder of 100 mesh sieves, is Flos Sophorae precious, packaging, sterilizing, fresh Flos Sophorae is 321 0.5 0.3 0.2 with the weight ratio of parching with earth RHIIZOMA DIOSCOREAE fr0m Henan of China, Pachyrhizua angulatus, Flos Rosae Rugosae, Pericarpium Citri Reticulatae and Radix Glycyrrhizae.
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| CN103494997A (en) * | 2013-10-18 | 2014-01-08 | 河南中医学院 | Traditional Chinese sophora flower medicine for treating hyperglycemia and diabetes |
| CN104887957A (en) * | 2015-06-11 | 2015-09-09 | 孙太辉 | Medicine for treating diabetes and hyperglycemia and preparation method of medicine |
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| CN103402506A (en) * | 2011-06-02 | 2013-11-20 | S·Y·列什科夫 | Combination for treatment of diabetes mellitus |
| CN103263449A (en) * | 2012-10-31 | 2013-08-28 | 奥·乌力吉 | Mongolian medicine for treatment of hyperglycemia and diabetes |
| CN103494997A (en) * | 2013-10-18 | 2014-01-08 | 河南中医学院 | Traditional Chinese sophora flower medicine for treating hyperglycemia and diabetes |
| CN104887957A (en) * | 2015-06-11 | 2015-09-09 | 孙太辉 | Medicine for treating diabetes and hyperglycemia and preparation method of medicine |
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