CN105902591A - Botanical disinfectant and preparation method thereof - Google Patents

Botanical disinfectant and preparation method thereof Download PDF

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Publication number
CN105902591A
CN105902591A CN201610475408.3A CN201610475408A CN105902591A CN 105902591 A CN105902591 A CN 105902591A CN 201610475408 A CN201610475408 A CN 201610475408A CN 105902591 A CN105902591 A CN 105902591A
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extract
parts
folium isatidis
herba chrysosplenii
chrysosplenii macrophylli
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CN201610475408.3A
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Chinese (zh)
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张育栋
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/27—Asclepiadaceae (Milkweed family), e.g. hoya
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/19—Acanthaceae (Acanthus family)
    • A61K36/195—Strobilanthes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/31—Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
    • A61K36/315—Isatis, e.g. Dyer's woad
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/70—Polygonaceae (Buckwheat family), e.g. spineflower or dock
    • A61K36/704—Polygonum, e.g. knotweed
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/85—Verbenaceae (Verbena family)
    • A61K36/855—Clerodendrum, e.g. glorybower
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH

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  • Health & Medical Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Biotechnology (AREA)
  • Botany (AREA)
  • Medical Informatics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The invention discloses a botanical disinfectant and a preparation method thereof. The botanical disinfectant is prepared from the following raw materials in parts by weight: 35-45 parts of marsdenia longipes extract, 25-35 parts of primula malacoides extract, 7-9 parts of chrysosplenium macrophyllum extract and indigowoad leaf extract, 12-14 parts of alcohol and 240-260 parts of deionized water, wherein the ratio of the chrysosplenium macrophyllum extract to the indigowoad leaf extract is equal to (6-8): 1 in parts by weight. The botanical disinfectant provided by the invention has an excellent antibacterial effect, and the antibacterial effect may be associated with the ratio of the chrysosplenium macrophyllum extract to the indigowoad leaf extract in parts by weight; and when the ratio of the chrysosplenium macrophyllum extract to the indigowoad leaf extract is equal to (6-8): 1 in parts by weight, the disinfection effect is most remarkable.

Description

A kind of plant source disinfectant solution and preparation method thereof
Technical field
The present invention relates to a kind of plant source disinfectant solution and preparation method thereof.
Background technology
When medical institutions carry out medical test, the bacterial flora system of checking system should minimize, and this is just to medical personnel originally Trier needed for body and inspection carries out omnibearing sterilizing thoroughly, eliminates a series of antibacterial and pathogen is golden Staphylococcus aureus, escherichia coli, Salmonella typhi, Pseudomonas aeruginosa, proteus vulgaris etc., provide for checking system Good environment.
Some patients are often extracted specimen and test by the section office that clinical laboratory chemically examines as hospital inspection, and check work to need Aseptically to carry out, to prevent antibacterial, fungus and virus etc. to be mixed into inspection specimen, thus to affect test effect, make Assay inaccuracy, and inspection institute use the bactericidal antiphlogistic medicine such as big multipair human body wound such as iodine tincture, ethanol, povidone iodine and Skin is irritant, and have even can cause allergic reaction, and even can cause the dermatosis such as dermatitis and eczema.
Summary of the invention
It is an object of the invention to provide a kind of plant source disinfectant solution and preparation method thereof.
The above-mentioned purpose of the present invention is achieved by techniques below scheme:
A kind of plant source disinfectant solution, is prepared from by the raw material of following weight portion: Radix Marsdeniae Longipedis extract, 35～45 parts；Herald spring Flower extract, 25～35 parts；Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract totally 7～9 parts；Ethanol, 12～14 parts；Deionized water, 240～260 parts；The weight ratio of described Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract is 6～8:1；
Wherein, Radix Marsdeniae Longipedis extract, Flos Primulae Vittatae extract, Herba Chrysosplenii Macrophylli extract are identical with the preparation method of Folium Isatidis extract, Comprise the steps: respectively
Step S1, by dry Radix Marsdeniae Longipedis, Flos Primulae Vittatae, Herba Chrysosplenii Macrophylli, Folium Isatidis with 70～80% alcohol heat reflux extract 1～2h；
Step S2, extracting solution filters, and concentrating under reduced pressure is spray-dried to obtain extract powder.
Further, described plant source disinfectant solution is prepared from by the raw material of following weight portion: Radix Marsdeniae Longipedis extract, 40 parts； Flos Primulae Vittatae extract, 30 parts；Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract totally 8 parts；Ethanol, 13 parts；Deionized water, 250 Part；The weight ratio of described Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract is 7:1.
Further, described plant source disinfectant solution is prepared from by the raw material of following weight portion: Radix Marsdeniae Longipedis extract, 35 parts； Flos Primulae Vittatae extract, 25 parts；Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract totally 7 parts；Ethanol, 12 parts；Deionized water, 240 Part；The weight ratio of described Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract is 6:1.
Further, described plant source disinfectant solution is prepared from by the raw material of following weight portion: Radix Marsdeniae Longipedis extract, 45 parts； Flos Primulae Vittatae extract, 35 parts；Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract totally 9 parts；Ethanol, 14 parts；Deionized water, 260 Part；The weight ratio of described Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract is 8:1.
The preparation method of above-mentioned plant source disinfectant solution:
First by ethanol and deionized water mix homogeneously, then add Radix Marsdeniae Longipedis extract, Flos Primulae Vittatae extract, Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract, to being completely dissolved and get final product.
Advantages of the present invention:
The disinfectant solution that the present invention provides has the fungistatic effect of excellence, and this may extract with Herba Chrysosplenii Macrophylli extract in raw material and Folium Isatidis The weight ratio of thing is relevant, and when the weight ratio of Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract is 6～8:1, Disinfection Effect is For remarkably.
Detailed description of the invention
Further illustrate the essentiality content of the present invention below in conjunction with embodiment, but do not limit scope with this.To the greatest extent The present invention is explained in detail by pipe with reference to preferred embodiment, it will be understood by those within the art that, can be to the present invention Technical scheme modify or equivalent, without deviating from the spirit and scope of technical solution of the present invention.
In following embodiment, Radix Marsdeniae Longipedis extract, Flos Primulae Vittatae extract, Herba Chrysosplenii Macrophylli extract and the preparation side of Folium Isatidis extract Method is identical, comprises the steps: respectively
Step S1, by dry Radix Marsdeniae Longipedis, Flos Primulae Vittatae, Herba Chrysosplenii Macrophylli, Folium Isatidis with 70～80% alcohol heat reflux extract 1～2h；
Step S2, extracting solution filters, and concentrating under reduced pressure is spray-dried to obtain extract powder.
Embodiment 1: the preparation of plant source disinfectant solution
Parts by weight of raw materials compares:
Radix Marsdeniae Longipedis extract, 40 parts；Flos Primulae Vittatae extract, 30 parts；Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract totally 8 parts；Second Alcohol, 13 parts；Deionized water, 250 parts；The weight ratio of described Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract is 7:1.
Preparation method:
First by ethanol and deionized water mix homogeneously, then add Radix Marsdeniae Longipedis extract, Flos Primulae Vittatae extract, Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract, to being completely dissolved and get final product.
Embodiment 2: the preparation of plant source disinfectant solution
Parts by weight of raw materials compares:
Radix Marsdeniae Longipedis extract, 35 parts；Flos Primulae Vittatae extract, 25 parts；Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract totally 7 parts；Second Alcohol, 12 parts；Deionized water, 240 parts；The weight ratio of described Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract is 6:1.
Preparation method:
First by ethanol and deionized water mix homogeneously, then add Radix Marsdeniae Longipedis extract, Flos Primulae Vittatae extract, Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract, to being completely dissolved and get final product.
Embodiment 3: the preparation of plant source disinfectant solution
Parts by weight of raw materials compares:
Radix Marsdeniae Longipedis extract, 45 parts；Flos Primulae Vittatae extract, 35 parts；Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract totally 9 parts；Second Alcohol, 14 parts；Deionized water, 260 parts；The weight ratio of described Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract is 8:1.
Preparation method:
First by ethanol and deionized water mix homogeneously, then add Radix Marsdeniae Longipedis extract, Flos Primulae Vittatae extract, Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract, to being completely dissolved and get final product.
Embodiment 4: the preparation of plant source disinfectant solution
Parts by weight of raw materials compares:
Radix Marsdeniae Longipedis extract, 40 parts；Flos Primulae Vittatae extract, 30 parts；Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract totally 8 parts；Second Alcohol, 13 parts；Deionized water, 250 parts；The weight ratio of described Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract is 6:1.
Preparation method:
First by ethanol and deionized water mix homogeneously, then add Radix Marsdeniae Longipedis extract, Flos Primulae Vittatae extract, Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract, to being completely dissolved and get final product.
Embodiment 5: the preparation of plant source disinfectant solution
Parts by weight of raw materials compares:
Radix Marsdeniae Longipedis extract, 40 parts；Flos Primulae Vittatae extract, 30 parts；Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract totally 8 parts；Second Alcohol, 13 parts；Deionized water, 250 parts；The weight ratio of described Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract is 8:1.
Preparation method:
First by ethanol and deionized water mix homogeneously, then add Radix Marsdeniae Longipedis extract, Flos Primulae Vittatae extract, Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract, to being completely dissolved and get final product.
Embodiment 6: comparative example, the weight ratio of Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract is 5:1
Parts by weight of raw materials compares:
Radix Marsdeniae Longipedis extract, 40 parts；Flos Primulae Vittatae extract, 30 parts；Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract totally 8 parts；Second Alcohol, 13 parts；Deionized water, 250 parts；The weight ratio of described Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract is 5:1.
Preparation method:
First by ethanol and deionized water mix homogeneously, then add Radix Marsdeniae Longipedis extract, Flos Primulae Vittatae extract, Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract, to being completely dissolved and get final product.
Embodiment 7: comparative example, the weight ratio of Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract is 9:1
Parts by weight of raw materials compares:
Radix Marsdeniae Longipedis extract, 40 parts；Flos Primulae Vittatae extract, 30 parts；Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract totally 8 parts；Second Alcohol, 13 parts；Deionized water, 250 parts；The weight ratio of described Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract is 9:1.
Preparation method:
First by ethanol and deionized water mix homogeneously, then add Radix Marsdeniae Longipedis extract, Flos Primulae Vittatae extract, Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract, to being completely dissolved and get final product.
Embodiment 8: effect example
Below the disinfectant solution of embodiment 1～7 preparation is carried out bacteriostatic experiment:
1, experimental strain:
Select the strain that laboratory is representative, staphylococcus aureus, candida albicans and bacillus pyocyaneus.
2, bacterium culture medium:
Nutrient agar and nutrient broth medium.
3, experiment classification:
Dividing experimental group and matched group, experimental group is the disinfectant solution of embodiment 1～7 preparation, and matched group selects hypochlorous acid disinfectant solution.
4, specific experiment step:
(1) preparation of bacterium solution
Three kinds of strains are inoculated on three agar culture mediums respectively, cultivate 36 hours at 37 DEG C, respectively from above-mentioned agar culture medium Middle picking colonies typical is inoculated in three fresh nutrient broth mediums respectively, synthermal under, cultivate 24 hours, standby.
(2) disinfectant solution is selected
The disinfectant solution of Example 1～7 preparation and each 45ml of hypochlorous acid disinfectant solution, be respectively placed in cut-back tank, the dilutest to it Release 30 times, dilute solution is placed in respectively in three containers, is placed under aseptic condition, standby.
(3) strain subpackage and labelling
The experimental strain that above-mentioned steps (1) is supported 24 hours through nutrition broth cultivation is diluted 50 times respectively with aseptic nutrient broth, Every kind of strain is sub-packed in two culture dishs, respectively labelling matched group and experimental group.
(4) sterilization
Dilute solution prepared by the embodiment 1～7 of above-mentioned steps (2) gained is respectively dropped into experimental group culture dish, and hypochlorous acid is sterilized Liquid dilute solution is respectively dropped into matched group culture dish.
5, experimental observation is measured:
Above-mentioned experiment is observed, observes three groups of strains and measure strain revolution mark, and recording observed data result.
The effect of embodiment 2,3 is basically identical with embodiment 4,5.
6, after observation is measured, it is placed in above-mentioned culture dish under aseptic condition standing two days, after liquid to be sterilized volatilizees completely, Follow the tracks of it has been observed that matched group bacterium colony continues breeding, and experimental group bacterium colony is unchanged.
7, experimental result:
The sterilizing ability of experimental group plant source of the present invention disinfectant solution is much larger than the sterilizing ability of matched group disinfectant solution, and can suppress Strain continues breeding.The present invention provide disinfectant solution have excellence fungistatic effect, this may with Herba Chrysosplenii Macrophylli extract in raw material and The weight ratio of Folium Isatidis extract is relevant, when the weight ratio of Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract is 6～8:1, Disinfection Effect is outstanding.
The effect of above-described embodiment indicates that the essentiality content of the present invention, but does not limit protection scope of the present invention with this. It will be understood by those within the art that, technical scheme can be modified or equivalent, and not take off Essence and protection domain from technical solution of the present invention.

Claims (5)

1. a plant source disinfectant solution, it is characterised in that be prepared from by the raw material of following weight portion: Radix Marsdeniae Longipedis extract, 35～45 parts；Flos Primulae Vittatae extract, 25～35 parts；Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract totally 7～9 parts；Ethanol, 12～14 Part；Deionized water, 240～260 parts；The weight ratio of described Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract is 6～8:1；
Wherein, Radix Marsdeniae Longipedis extract, Flos Primulae Vittatae extract, Herba Chrysosplenii Macrophylli extract are identical with the preparation method of Folium Isatidis extract, Comprise the steps: respectively
Step S1, by dry Radix Marsdeniae Longipedis, Flos Primulae Vittatae, Herba Chrysosplenii Macrophylli, Folium Isatidis with 70～80% alcohol heat reflux extract 1～2h；
Step S2, extracting solution filters, and concentrating under reduced pressure is spray-dried to obtain extract powder.
Plant source disinfectant solution the most according to claim 1, it is characterised in that be prepared from by the raw material of following weight portion: Radix Marsdeniae Longipedis extract, 40 parts；Flos Primulae Vittatae extract, 30 parts；Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract totally 8 parts；Ethanol, 13 parts；Deionized water, 250 parts；The weight ratio of described Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract is 7:1.
Plant source disinfectant solution the most according to claim 1, it is characterised in that be prepared from by the raw material of following weight portion: Radix Marsdeniae Longipedis extract, 35 parts；Flos Primulae Vittatae extract, 25 parts；Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract totally 7 parts；Ethanol, 12 parts；Deionized water, 240 parts；The weight ratio of described Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract is 6:1.
Plant source disinfectant solution the most according to claim 1, it is characterised in that be prepared from by the raw material of following weight portion: Radix Marsdeniae Longipedis extract, 45 parts；Flos Primulae Vittatae extract, 35 parts；Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract totally 9 parts；Ethanol, 14 parts；Deionized water, 260 parts；The weight ratio of described Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract is 8:1.
5. the preparation method of the arbitrary described plant source disinfectant solution of Claims 1 to 4, it is characterised in that:
First by ethanol and deionized water mix homogeneously, then add Radix Marsdeniae Longipedis extract, Flos Primulae Vittatae extract, Herba Chrysosplenii Macrophylli extract and Folium Isatidis extract, to being completely dissolved and get final product.
CN201610475408.3A 2016-06-22 2016-06-22 Botanical disinfectant and preparation method thereof Pending CN105902591A (en)

Priority Applications (1)

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105079211A (en) * 2015-08-11 2015-11-25 金科伟业（中国）有限公司 Antibacterial and anti-inflammation traditional Chinese medicine extract and application and preparation thereof
CN105233184A (en) * 2015-11-23 2016-01-13 高秀明 Disinfectant containing Traditional Chinese medicine ingredients

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105079211A (en) * 2015-08-11 2015-11-25 金科伟业（中国）有限公司 Antibacterial and anti-inflammation traditional Chinese medicine extract and application and preparation thereof
CN105233184A (en) * 2015-11-23 2016-01-13 高秀明 Disinfectant containing Traditional Chinese medicine ingredients

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