CN105901381A - Feed additive for preventing avian influenza and preparation method thereof - Google Patents
Feed additive for preventing avian influenza and preparation method thereof Download PDFInfo
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- CN105901381A CN105901381A CN201610260988.4A CN201610260988A CN105901381A CN 105901381 A CN105901381 A CN 105901381A CN 201610260988 A CN201610260988 A CN 201610260988A CN 105901381 A CN105901381 A CN 105901381A
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- feed additive
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- aspergillus niger
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Abstract
The invention relates to a feed additive for preventing avian influenza; the feed additive is composed of the following raw materials in parts by weight: 30-60 parts of folium isatidis, 20-40 parts of common St.John's wort herb, 10-30 parts of radix astragali, and 10-25 parts of liquorice. The invention also discloses a preparation method of the feed additive. The feed additive not only can prevent and control avian influenza but also can improve the immunity of animals, and has the characteristics of small side effects, low cost, simple process and easy industrialization.
Description
Technical field
The present invention relates to field of feed additive technology, particularly relate to a kind of feed additive preventing and treating bird flu and system thereof
Preparation Method.
Background technology
Bird flu (Avian Influenza, AI) is caused by orthomyxoviridae family's Influenza Virus influenza A virus
Birds infects, and shows as the systemic infection of high incidence and high mortality, serious threat aviculture all over the world.Owing to fowl is flowed
The variation many, easy of Influenza Virus gene segment and restructuring, thus cause chemicals and vaccine to be all difficult to play the optimal anti-of them
Control effect.Under the ideological trend of current back to nature, food-safety problem is increasingly paid attention to by the world, the green preventing and treating of fowl diseases
Become main flow.
China's Chinese herbal medicine resource enriches, and Chinese herbal medicine has suppression virus replication, regulation immunologic function, stops virus to cause cell
The comprehensive effect such as pathological changes and easing pain and diminishing inflammation, and low toxic and side effects.Therefore, Chinese herbal medicine has wide for the preventing and treating of bird flu
Development prospect.
Summary of the invention
The technical problem to be solved is to provide a kind of effect and significantly prevents and treats the feed additive of bird flu.
Another technical problem to be solved by this invention is to provide the preparation side of the feed additive of this preventing and treating bird flu
Method.
For solve the problems referred to above, a kind of feed additive preventing and treating bird flu of the present invention, it is characterised in that: it by
The raw material composition of following weight portion: Folium Isatidis 30 ~ 60 parts, Herba Hyperici perforati 20 ~ 40 parts, the Radix Astragali 10 ~ 30 parts, 10 ~ 25 parts of Radix Glycyrrhizae.
The preparation method of a kind of feed additive preventing and treating bird flu as above, comprises the following steps:
(1) weigh by proportioning;
(2) after Folium Isatidis, Herba Hyperici perforati, the Radix Astragali, Radix Glycyrrhizae mix homogeneously, it is crushed to 40 ~ 120 mesh, obtains medical material powder;
(3) prepare fermented liquid:
1 L distilled water is sequentially added into carbamide 0.1 ~ 0.5 g, ammonium sulfate 1.0 ~ 2.0 g, potassium dihydrogen phosphate 1.0 ~ 3.0 g,
Calcium chloride 0.3 ~ 0.8 g, magnesium sulfate 0.2 ~ 0.6 g, ferrous sulfate 2.0 ~ 7.0 mg, manganese sulfate 1.0 ~ 3.0 mg, zinc sulfate 1.0
~ 3.0 mg, cobaltous chloride 10 ~ 30mg, mix homogeneously, obtain fermented liquid;
(4) aspergillus niger seed culture fluid:
By 100 μ L Aspergillus niger suspension inoculations to potato dextrose agar, 30 DEG C of constant incubators are cultivated 3d,
A layer thickness to be grown uniform black spore;Then slant pore 3 is inoculated by every 150ml potato dextrose broth
Ring, shakes cultivation 24h at 30 DEG C with the speed of 130r/min, obtains aspergillus niger seed culture fluid;
(5) add fermented liquid described in 200 mL according to medical material powder described in every 100 g, and inoculate aspergillus niger seed described in 10 mL
Culture fluid, obtains mixed liquor;Described mixed liquor shakes cultivation 10 ~ 100 hours at 10 ~ 45 DEG C with the speed of 100 ~ 500 r/min,
Obtain the medicine after fermentation;
(6) after the medicine after described fermentation being dried 12 ~ 24h at a temperature of 35 ~ 65 DEG C, size-reduced, cross 100 mesh sieves and get final product.
Described step (4) middle potato dextrose agar refers to 200g peeling potatoes stripping and slicing, adds 1L distillation
Water, boils after 10 ~ 20min by filtered through gauze, and adds distilled water to 1L;It is subsequently adding 20g glucose and 20g agar, heating
Through 121 DEG C of sterilizing 20min and get final product after fusing.
Described step (4) middle potato dextrose broth refers to, by 200g peeling potatoes stripping and slicing, add 1L distilled water, boil
By filtered through gauze after boiling 10 ~ 20min, and add distilled water to 1L;It is subsequently adding 20g glucose, goes out through 121 DEG C after adding heat fusing
Bacterium 20min and get final product.
The present invention compared with prior art has the advantage that
1, the present invention selects the Radix Astragali, Radix Glycyrrhizae to join Folium Isatidis, Herba Hyperici perforati, mends and settles accounts conjunction, mends and does not help heat, is not just the most hindering;And wherein
Folium Isatidis, Herba Hyperici perforati primary efficacy be antiviral, antibacterial, heat-clearing and toxic substances removing, diuresis, profit larynx;The Radix Astragali, Radix Glycyrrhizae mainly strengthen machine
Body resistance, promotion immunity, promotion antibody tormation and the raising of resistance against diseases, therefore, this Chinese medicine composition selects medical material reasonable.
2, the present invention is produced by aspergillus niger cellulolytic enzyme, hemicellulose catabolic enzyme destroy plant amedica cell wall knot
Structure, such that it is able to increase substantially active constituent content and the animal utilization ratio to effective ingredient in medical material.Through efficient liquid phase
Chromatograph detection shows, its principle active component 4 (3) H quinazolone, hypericin, astragalus polysaccharides and the content of liquirtin after fermentation
It is respectively increased 12%, 30%, 8.2% and 5.8%.
3, the present invention is notable through test preventing and treating bird flu effect.
Test method: take 2 week old chickling 200,100 feeding normal diets;100 feedings contain embodiment 1 to be prepared
Chinese herbal medicine additive feedstuff.To two groups of chickling intramuscular injection bird flu (AV) after 24 hours, mortality rate (ginseng in recording 2 weeks
It is shown in Table 1).
Table 1
Result of the test shows, the chickling mortality rate of feeding Chinese medicine feed additive is substantially less than matched group.
4, the present invention can prevent and treat bird flu, can improve again the immunity of animal, the most also has that side effect is little, cost
Low, technique is simple, it is easy to the features such as industrialization.
Detailed description of the invention
Embodiment 1 one kinds prevents and treats the feed additive of bird flu, and it is made up of the raw material of following weight portion (g): Folium Isatidis
40 parts, Herba Hyperici perforati 30 parts, the Radix Astragali 15 parts, 15 parts of Radix Glycyrrhizae.
The preparation method of the feed additive of this preventing and treating bird flu, comprises the following steps:
(1) weigh by proportioning;
(2) after Folium Isatidis, Herba Hyperici perforati, the Radix Astragali, Radix Glycyrrhizae mix homogeneously, it is crushed to 50 mesh, obtains medical material powder;
(3) prepare fermented liquid:
1 L distilled water is sequentially added into carbamide 0.2 g, ammonium sulfate 1.3 g, potassium dihydrogen phosphate 2.2 g, calcium chloride 0.5 g,
Magnesium sulfate 0.4 g, ferrous sulfate 3.5 mg, manganese sulfate 2.0 mg, zinc sulfate 1.8 mg, cobaltous chloride 18mg, mix homogeneously, to obtain final product
Fermented liquid;
(4) aspergillus niger seed culture fluid:
By 100 μ L Aspergillus niger suspension inoculations to potato dextrose agar, 30 DEG C of constant incubators are cultivated 3d,
A layer thickness to be grown uniform black spore;Then slant pore 3 is inoculated by every 150ml potato dextrose broth
Ring, shakes cultivation 24h at 30 DEG C with the speed of 130r/min, obtains aspergillus niger seed culture fluid;
(5) add 200 mL fermented liquids according to every 100 g medical material powder, and inoculate 10 mL aspergillus niger seed culture fluids, mixed
Close liquid;Mixed liquor shakes cultivation 24 hours at 28 DEG C with the speed of 400 r/min, obtains the medicine after fermentation;
(6) after the medicine after fermentation being dried 18h at a temperature of 45 DEG C, size-reduced, mistake 100 mesh sieves and get final product.
During use, this feed additive is added in feedstuff with the amount of 15% and mixes.
Embodiment 2 one kinds prevents and treats the feed additive of bird flu, and it is made up of the raw material of following weight portion (g): Folium Isatidis
30 parts, Herba Hyperici perforati 20 parts, the Radix Astragali 10 parts, 10 parts of Radix Glycyrrhizae.
The preparation method of the feed additive of this preventing and treating bird flu, comprises the following steps:
(1) weigh by proportioning;
(2) after Folium Isatidis, Herba Hyperici perforati, the Radix Astragali, Radix Glycyrrhizae mix homogeneously, it is crushed to 40 mesh, obtains medical material powder;
(3) prepare fermented liquid:
1 L distilled water is sequentially added into carbamide 0.1 g, ammonium sulfate 1.0 g, potassium dihydrogen phosphate 1.0 g, calcium chloride 0.3 g,
Magnesium sulfate 0.2 g, ferrous sulfate 2.0 mg, manganese sulfate 1.0 mg, zinc sulfate 1.0 mg, cobaltous chloride 10mg, mix homogeneously, to obtain final product
Fermented liquid;
(4) aspergillus niger seed culture fluid:
By 100 μ L Aspergillus niger suspension inoculations to potato dextrose agar, 30 DEG C of constant incubators are cultivated 3d,
A layer thickness to be grown uniform black spore;Then slant pore 3 is inoculated by every 150ml potato dextrose broth
Ring, shakes cultivation 24h at 30 DEG C with the speed of 130r/min, obtains aspergillus niger seed culture fluid;
(5) add 200 mL fermented liquids according to every 100 g medical material powder, and inoculate aspergillus niger seed culture fluid described in 10 mL,
To mixed liquor;Mixed liquor shakes cultivation 100 hours at 10 DEG C with the speed of 100 r/min, obtains the medicine after fermentation;
(6) after the medicine after fermentation being dried 12h at a temperature of 35 DEG C, size-reduced, mistake 100 mesh sieves and get final product.
During use, this feed additive is added in feedstuff with the amount of 5% and mixes.
Embodiment 3 one kinds prevents and treats the feed additive of bird flu, and it is made up of the raw material of following weight portion (g): Folium Isatidis
60 parts, Herba Hyperici perforati 40 parts, the Radix Astragali 30 parts, 25 parts of Radix Glycyrrhizae.
The preparation method of the feed additive of this preventing and treating bird flu, comprises the following steps:
(1) weigh by proportioning;
(2) after Folium Isatidis, Herba Hyperici perforati, the Radix Astragali, Radix Glycyrrhizae mix homogeneously, it is crushed to 120 mesh, obtains medical material powder;
(3) prepare fermented liquid:
Carbamide 0.5 g, ammonium sulfate 2.0 g, potassium dihydrogen phosphate 3.0 g, calcium chloride 0.8 g, sulfur it is sequentially added in 1 L distilled water
Acid magnesium 0.6 g, ferrous sulfate 7.0 mg, manganese sulfate 3.0 mg, zinc sulfate 3.0 mg, cobaltous chloride 30mg, mix homogeneously, must send out
Ferment liquid;
(4) aspergillus niger seed culture fluid:
By 100 μ L Aspergillus niger suspension inoculations to potato dextrose agar, 30 DEG C of constant incubators are cultivated 3d,
A layer thickness to be grown uniform black spore;Then slant pore 3 is inoculated by every 150ml potato dextrose broth
Ring, shakes cultivation 24h at 30 DEG C with the speed of 130r/min, obtains aspergillus niger seed culture fluid;
(5) add 200 mL fermented liquids according to every 100 g medical material powder, and inoculate aspergillus niger seed culture fluid described in 10 mL,
To mixed liquor;Mixed liquor shakes cultivation 10 hours at 45 DEG C with the speed of 500 r/min, obtains the medicine after fermentation;
(6) after the medicine after fermentation being dried 24h at a temperature of 65 DEG C, size-reduced, mistake 100 mesh sieves and get final product.
During use, this feed additive is added in feedstuff with the amount of 20% and mixes.
In above-described embodiment 1 ~ 3, potato dextrose agar refers to 200g peeling potatoes stripping and slicing, adds 1L and steams
Distilled water, boils after 10 ~ 20min by filtered through gauze, and adds distilled water to 1L;It is subsequently adding 20g glucose and 20g agar, adds
Through 121 DEG C of sterilizing 20min and get final product after heat fusing.
Potato dextrose broth refers to, by 200g peeling potatoes stripping and slicing, add 1L distilled water, after boiling 10 ~ 20min
By filtered through gauze, and add distilled water to 1L;It is subsequently adding 20g glucose, through 121 DEG C of sterilizing 20min and get final product after adding heat fusing.
Claims (4)
1. the feed additive preventing and treating bird flu, it is characterised in that: it is made up of the raw material of following weight portion: Folium Isatidis 30
~ 60 parts, Herba Hyperici perforati 20 ~ 40 parts, the Radix Astragali 10 ~ 30 parts, 10 ~ 25 parts of Radix Glycyrrhizae.
The preparation method of a kind of feed additive preventing and treating bird flu the most as claimed in claim 1, comprises the following steps:
(1) weigh by proportioning;
(2) after Folium Isatidis, Herba Hyperici perforati, the Radix Astragali, Radix Glycyrrhizae mix homogeneously, it is crushed to 40 ~ 120 mesh, obtains medical material powder;
(3) prepare fermented liquid:
1 L distilled water is sequentially added into carbamide 0.1 ~ 0.5 g, ammonium sulfate 1.0 ~ 2.0 g, potassium dihydrogen phosphate 1.0 ~ 3.0 g,
Calcium chloride 0.3 ~ 0.8 g, magnesium sulfate 0.2 ~ 0.6 g, ferrous sulfate 2.0 ~ 7.0 mg, manganese sulfate 1.0 ~ 3.0 mg, zinc sulfate 1.0
~ 3.0 mg, cobaltous chloride 10 ~ 30mg, mix homogeneously, obtain fermented liquid;
(4) aspergillus niger seed culture fluid:
By 100 μ L Aspergillus niger suspension inoculations to potato dextrose agar, 30 DEG C of constant incubators are cultivated 3d,
A layer thickness to be grown uniform black spore;Then slant pore 3 is inoculated by every 150ml potato dextrose broth
Ring, shakes cultivation 24h at 30 DEG C with the speed of 130r/min, obtains aspergillus niger seed culture fluid;
(5) add fermented liquid described in 200 mL according to medical material powder described in every 100 g, and inoculate aspergillus niger seed described in 10 mL
Culture fluid, obtains mixed liquor;Described mixed liquor shakes cultivation 10 ~ 100 hours at 10 ~ 45 DEG C with the speed of 100 ~ 500 r/min,
Obtain the medicine after fermentation;
(6) after the medicine after described fermentation being dried 12 ~ 24h at a temperature of 35 ~ 65 DEG C, size-reduced, cross 100 mesh sieves and get final product.
The preparation method of a kind of feed additive preventing and treating bird flu the most as claimed in claim 2, it is characterised in that: described step
Rapid (4) middle potato dextrose agar refers to, by 200g peeling potatoes stripping and slicing, add 1L distilled water, boil 10 ~ 20min
Afterwards by filtered through gauze, and add distilled water to 1L;It is subsequently adding 20g glucose and 20g agar, goes out through 121 DEG C after adding heat fusing
Bacterium 20min and get final product.
The preparation method of a kind of feed additive preventing and treating bird flu the most as claimed in claim 2, it is characterised in that: described step
Rapid (4) middle potato dextrose broth refers to, by 200g peeling potatoes stripping and slicing, add 1L distilled water, uses after boiling 10 ~ 20min
Filtered through gauze, and add distilled water to 1L;It is subsequently adding 20g glucose, through 121 DEG C of sterilizing 20min and get final product after adding heat fusing.
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Cited By (2)
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CN107536861A (en) * | 2017-08-15 | 2018-01-05 | 中国科学院近代物理研究所 | A kind of folium isatidis exempts to decoct the preparation method of particle |
CN115413734A (en) * | 2022-07-13 | 2022-12-02 | 文山壮族苗族自治州农业科学院 | Antibacterial and antiviral Chinese herbal medicine feed additive, preparation method and application thereof |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107536861A (en) * | 2017-08-15 | 2018-01-05 | 中国科学院近代物理研究所 | A kind of folium isatidis exempts to decoct the preparation method of particle |
CN115413734A (en) * | 2022-07-13 | 2022-12-02 | 文山壮族苗族自治州农业科学院 | Antibacterial and antiviral Chinese herbal medicine feed additive, preparation method and application thereof |
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