CN105891523A - AIDS-diagnosis-used cheap microflow control device for transferring liquid flow in new mode - Google Patents
AIDS-diagnosis-used cheap microflow control device for transferring liquid flow in new mode Download PDFInfo
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- CN105891523A CN105891523A CN201510035312.0A CN201510035312A CN105891523A CN 105891523 A CN105891523 A CN 105891523A CN 201510035312 A CN201510035312 A CN 201510035312A CN 105891523 A CN105891523 A CN 105891523A
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Abstract
The invention relates to an AIDS-diagnosis-used cheap microflow control device for transferring liquid flow in a new mode and belongs to the field of analytical tests. When polydimethylsiloxane (PDMS) which is low in price and extremely easy to process is adopted to make a substrate of an AIDS-diagnosis-used cheap microflow control chip, a series of problems are caused; the surface of the PDMS material is highly hydrophobic, and the effect of targeted surface modification or surface decoration is difficult to endure; the device aims to solve the mentioned relevant problems. The device is mainly characterized in that PDMS having the originally-ecological surface is selected for making a substrate, a miniature ultrasonic transducer is arranged at the position, near a sample liquid flow terminal, of a microflow control chip in an attached mode, interfacial tension is lowered through ultrasonic waves, and meanwhile high absorption capacity of PDMS to the ultrasonic waves is utilized, so that the effect that the intensity of the ultrasonic waves is gradually decreased within a short distance rapidly is achieved, thereby interfacial tension difference is formed at the two ends of the chip, the pressure difference is formed at the two ends due to the interfacial tension difference, and the pressure difference drives the sample liquid flow to flow toward the terminal along a capillary tube channel.
Description
Technical field
The present invention relates to a kind of cheap micro fluidic device of the AIDS diagnosis transmitting liquid stream in a new way, belong to
Analyze field tests.
Background technology
Associated multi-channel micro-fluidic AIDS diagnosis technical background, may refer to CN 200910151219.0
Deng application for a patent for invention case.
Only for itself overall general picture of microflow control technique, may refer to famous micro-fluidic expert Lin Ping Cheng
The monograph " diagram Microfluid based Lab on a chip " that Mr. goes out not long ago, this monograph is gone out by Science Press
Version, this monograph to past of microflow control technique, now, and, vision of the future etc. aspect, suffer from detail
Most, to be deep into detail long discussion.
So, the Important Problems that this case to be had a talk is paid close attention to.
The basic framework of micro-fluidic chip, including being etched with the substrate of small fluid course and being fitted in therewith
Cover plate together, the small fluid course on described substrate, before fit on cover plate, apparent on see and be exactly
Some micro-channel, after waiting until to cover cover plate thereon, just the real Guan Bi described small liquid stream of formation leads to
Road, the conduit inner surface of this micro-channel constitutes described together with the part cover plate that surround this micro-channel
Small fluid course;So, it is clear that assemble this small fluid course after completing, its inner surface
The major part of area is the inner surface area of that micro-channel, in other words, and the shape of this micro-channel inner surface
State or character substantially determine integrality or the character of this small fluid course;Therefore saying, this builds
Inner surface state or the inner surface character of the micro-channel on substrate are key factors;In principle, any
The material of its solid forms be can keep or substantially keep, substrate and cover plate can be used to make, such as,
The material that can act as substrate and cover plate can be monocrystalline silicon piece, piezoid, sheet glass, high polymer the most poly-two
Methylsiloxane, polymethyl methacrylate, Merlon etc.;Certainly, the selection of substrate and cover plate
Selection can be identical, it is also possible to differs;From material consumption, manufacture difficulty and application popularization prospect etc.
From the point of view of aspect, between these materials, there is the selection of the least difference, especially that substrate, affect bigger.
In various substrates make material, polydimethylsiloxane, i.e. PDMS, the easiest
Molding, makes micro-channel extremely simple on such substrate, and this lower cost for material, with this poly-two
Methylsiloxane material makes substrate, suppresses or etch micro-channel thereon, and with glass or polypropylene or its
The cover plate that the cheap materials such as its plastic sheet make matches, and is a kind of more satisfactory selection seemingly;Certainly,
Patch material can also select to use cheap polydimethyl siloxane material: so, and this substrate selection is
The scheme of polydimethyl siloxane material, material is extremely cheap, makes extremely simple, seem should also be as being extremely easy to general
And, promote.
But, thing is really not so simply.
One, this polydimethyl siloxane material, the material that i.e. abbreviation letter PDMS is referred to, its
Body is the most hydrophobic a kind of material, builds micro-channel, if do not carried out for this microflute on this material
The modified operation on surface, road, then, after entirety has been assembled, after i.e. covering cover plate, because of the institute in structure
State its inner surface of micro-channel and occupy the inner surface of most fluid course, then, this PDMS micro-channel
Its strong hydrophobic property of inner surface, is deciding factor, and it can make to be similar to the polar liquid of aqueous solution
Fine liquid stream by becoming the most difficult, its flow resistance is big, and the most general Micropump is all difficult to promote,
Certainly, if cover plate also selects to use this PDMS material, then, problem is substantially identical, similar;
Therefore, among prior art, it is modified repairing particular for the micro-channel inner surface on this PDMS material
Decorations, are necessary operations;So, this operates pretty troublesome for the modification of PDMS micro-channel inner surface?
That is not this problem, constitutes serious technical puzzlement, is another problem: this PDMS material
The PDMS polymer molecule of its body of substrate inside mutually has the characteristic automatically diffusing to the surface, migrating, this
The substrate body characteristic that internal PDMS polymer molecule automatically diffuses to the surface, migrates mutually, will make through table
When state after the modification of that its inner surface of micro-channel of face modifying and decorating can not maintain sufficiently long
Between, holding time of its inner surface state of the micro-channel after that is surface-modified has substantially been only sufficient to experiment
The time of chamber interior test experiments needs;In other words, this PDMS modified through surface modification or surface
Micro-channel inner surface, after its modification or say the apparent condition formed after modification can not be lasting, but
Automatically tend to soon or say the apparent condition become again before surface modification, in relatively short period of time, returning to that
The most hydrophobic apparent condition kind originally, then, just think, such micro-fluidic chip can make in a large number,
Mass storage, being widely popularized, answer is clearly, that is, it is impossible to.On this PDMS material
Micro-channel, does not do if surface modifies, and is similar to the fine liquid stream of polar solvent of aqueous solution and cannot pump and pass through,
Chip the most just cannot use;And if done surface modification, cannot persistently keep again its modify after state,
The most equally cannot popularization and application.
So, how to accomplish to utilize cheap PDMS material to make substrate, and institute can be released
State micro-channel inner surface decorating state cannot persistently, chip cannot make in a large number, in a large number deposit so that extensively push away
The most such a puzzlement making the numerous professional in this area be entangled with for a long time, it is simply that its obvious technology
The highly difficult problem that obstacle can not be despised.
Having there are a lot of days in this highly difficult problem, up to now, is not yet properly settled.
Its two, the PDMS material of non-surface modification, above it is stated that, its surface is the most hydrophobic, this
Plant the most hydrophobic material surface and also have another problem, that is, the most hydrophobic this PDMS
Biomacromolecule can be adsorbed in surface, and, these adsorbed biomacromolecules also can be further at PDMS
Depression further on surface, gradually falls into gradually deep, until within the heavy body phase being trapped in PDMS substrate, its
Real, this process, partly it is also due to PDMS material body phase interior polymeric thing molecule and has to surface expansion
Dissipate, travel motion is caused;This situation, it is also possible to explain from another angle, i.e. constant
Ground is internal to its diffusion into the surface, those polymer molecules of migration mutually by PDMS body, the result of its motion,
Within being the body phase that little by little those have been involved in by the biomacromolecule of surface adsorption PDMS substrate, letter
Singly saying, these adsorbed biomacromolecules are swallowed up mutually by PDMS substrate body exactly;So, this
PDMS substrate body swallows up the phenomenon of biomacromolecule mutually, its impact caused, and necessarily causes relating to raw
The severe deviations of all kinds of test data of experiment of thing macromole.
As it has been described above, the problem of PDMS substrate is, its not only surface adsorption biomacromolecule, and swallow up
Biomacromolecule, so, its disappearance of biomacromolecule as experiment test object will not be because of surface
Saturated absorption and stop, but, be constantly adsorbed, the most constantly swallowed up.
About PDMS substrate in related experiment test process its body the most constantly swallow up test associated biomolecule divide greatly
The phenomenon of son, another kind of explanation is to say, PDMS body mutually in there is substantial amounts of Minute pores, associated biomolecule is big
Molecule is by after surface adsorption, and depression enters these Minute pores, and then is swallowed up;But, this case is invented
People thinks, those can allow that the air molecule of miniature scale clamp-ons described Minute pores therebetween, is not equal to
Saying that they also can directly allow that the biomacromolecule of relatively large yardstick enters, both difference on yardstick are huge,
Must not make sweeping generalizations.Bypass explanation, in any case, analyze the biomacromolecule quilt of object as dependence test
PDMS substrate micro-channel inner surface adsorbs, and then is constantly swallowed up by PDMS substrate body phase, and this is known
The phenomenon of objective reality.
In order to stop the effect of swallowing up relative to biomacromolecule of this PDMS substrate body, can be from containment
The absorption of biomacromolecule is addressed by PDMS surface, and way is aiming at this PDMS material surface
It is chemically modified modification, from the point of view of during with PDMS as substrate material, it is simply that to described microflute
The surface of road part is chemically modified modification, through the described micro-channel inner surface that chemical modification is modified, energy
Enough contain its absorption to biomacromolecule, and then avoid biomacromolecule to be swallowed up by PDMS substrate body phase;
But, or that old problem, that is, the table after chemical modification modification on PDMS material surface
Surface state cannot persistently keep, the internal mutually polymer molecule of this PDMS substrate body its automatically diffuse to the surface,
The process migrated, can become again that through the micro-channel inner surface state that surface chemical modification is modified soon
The most hydrophobic and the state of strong adsorption biomacromolecule originally, in other words, no matter this field is professional
How personnel turn from side to side, and its micro-channel inner surface of this PDMS substrate is the most rapidly to strong hydrophobic surface shape
State develops.
So, it is the best how can either to obtain that PDMS material price is the cheapest, substrate makes
Place, can reach again long-term this PDMS substrate micro-channel inner surface absorption process to biomacromolecule of containment,
And then stoping the effect of swallowing up of PDMS substrate body Relative biological macromole so that related chip manufactured goods can
Maintain a shelf-life the most long, rational, it is simply that a difficult problem the most thorny.This difficult problem is such as
The same with another difficult problem addressed above, make the numerous professional in this area be entangled with for a long time, perplex equally,
This difficult problem is the highly difficult problem that its obvious technology barrier can not be despised equally.This difficult problem is the most
There are a lot of days, up to now, the most not yet properly settled.
Summary of the invention
The technical problem to be solved is to provide a package solution, solves totally
Two difficult problems certainly addressed above, and, this solution is applied to AIDS diagnosis multichannel miniflow
Control chip field, forms a kind of novel AIDS diagnosis multichannel micro-fluidic device.
The present invention solves described technical problem by following scheme, and the device that the program provides is a kind of with new side
The cheap micro fluidic device of AIDS diagnosis of formula transmission liquid stream, the structure of this micro fluidic device includes multichannel
Micro-fluidic chip, the structure of this micro-fluidic chip includes bonded to each other being installed in substrate together and cover plate, institute
Stating substrate and cover plate is plate object or tablet, that face towards this cover plate of this substrate is contained via mould
The channel structure that pressure technique or etching technics are formed, this substrate is possibly together with being connected with this channel structure and piercing
The window structure formed via mould pressing process, etching technics or simple drilling technology of this substrate, bonded to each other
This substrate being installed together and this cover plate have been built into jointly containing pipeline configuration and the liquid pool that is attached thereto
The micro-fluidic chip of structure, the locations of structures of this pipeline is positioned at the boundary zone that this substrate is bonded to each other with this cover plate
Territory, its side of this window is blocked by this cover plate and opposite side is open, and the locations of structures of this window is exactly described liquid
The locations of structures in pond, the quantity of described liquid pool is three, and it is micro-that two its locations of structures of liquid pool therein are positioned at this
The sample introduction end of fluidic chip, remaining its locations of structures of liquid pool is positioned at the actual sample introduction of this micro-fluidic chip and surveys
The terminal of liquid flowing in its chip during examination, this terminal is located remotely from each other with this sample introduction end, one end warp of this pipeline
By be similarly positioned in the manifold shape turnout of this interface zone bonded to each other respectively with these two liquid being positioned at sample introduction end
Pond UNICOM, this remaining liquid pool of the other end of this pipeline and the described terminal being positioned at this micro-fluidic chip
UNICOM, and, the working electrode that is sequentially respectively installed in this pipeline on diverse location and to electrode and
Reference electrode, described working electrode is by conductive electrode and the embedding that is attached on this conductive electrode Chinese mugwort
The gold size sensitive membrane growing disease-specific antigen is constituted, and the structure of this pipeline is parallel construction, described in structure in parallel
The pipeline made is made up of four lateral parallel connections, and the quantity of described working electrode is four, these four work
The installation position of electrode lays respectively in described four laterals, and, these four its top layers of working electrode
Specific antigen in gold size sensitive membrane structure is four kinds of AIDSs that can be specific binding to aids antibody respectively
Sick antigenic substance, these four kinds of antigenic substances be respectively acquired immune deficiency syndrome (AIDS) specific antigen p24, gp41, gp120 and
Gp36, its material of described working electrode is gold material or thermal decomposition conducting polymer material, described work electricity
Extremely pattern presents lamellar or thread, it is important that, its material of this substrate is polydimethylsiloxane material, should
Its surface of substrate is the surface of primary form, the surface of this primary form its be intended to refer to not through any
The surface of the primary form of this material of surface chemical modification or any surface chemical modification, this micro fluidic device
Structure also include miniature ultrasonic transducer units, and, higher-order of oscillation electric signal transmission cable, this high frequency vibrating
The one end swinging electric signal transmission cable links together with this miniature ultrasonic transducer units, and this miniature ultrasonic changes
The cover plate of this micro-fluidic chip or the position of the neighbouring described terminal of substrate can be installed in attaching by device;This is miniature
Its major function of ultrasonic transducer is when the actual sample introduction of micro-fluidic chip is tested, and utilize that it is launched is super
Sound wave reduces the interfacial tension between the inwall of sample solution and described pipeline so that it is can be compatible, and,
Utilize the distance difference between described sample introduction end and described terminal and this miniature ultrasonic transducer units installation position
And the difference on its ultrasonic intensity experienced, its interfacial tension of sample introduction end and institute described in induced synthesis
Stating the difference between its interfacial tension of terminal, the interfacial tension difference between these two ends of this micro-fluidic chip can be
Forming pressure gap between these two ends of this micro-fluidic chip, this pressure gap can drive sample solution to described
End flow;Its function of this miniature ultrasonic transducer units also includes checking in sample with its ultrasound wave launched
Contained its absorption on described inner surface of pipeline of biomacromolecule, and then check this polydimethylsiloxanes
Its body of the substrate of alkane material is relative to the effect of swallowing up of this biomacromolecule;Described polydimethylsiloxane material
Its function of substrate includes and cover plate and working electrode and electrode and reference electrode are together built this micro-fluidic core
Sheet, soft and this polydimethylsiloxane material of having elasticity its function of substrate also includes with it ultrasound wave
The strong character absorbed, absorbs strongly to ultrasound wave, and thereby in this terminal of this micro-fluidic chip to this
The rapid decrement of ultrasonic intensity is realized within limited short distance between sample introduction end.
Described gold size sensitive membrane is chitosan gold size solution and acquired immune deficiency syndrome (AIDS) specific antigen solution to be sufficiently mixed all
Even, with point sample instrument point sample or coat specified structure position, and make its drying and forming-film form.Described gold size is quick
Acquired immune deficiency syndrome (AIDS) specific antigen in sense film is horseradish peroxidase or the AIDS of glucose oxidase labelling
Sick antigen, described gold size sensitive membrane has been included as fixing above-mentioned each acquired immune deficiency syndrome (AIDS) specific antigen and has introduced therein
Complementary medium, described complementary medium such as chitosan, cellulose acetate, gelatin one therein or it
Mixture.
Described pipeline in described microfluidic chip structure and described lateral and described manifold shape trouble
Road, its internal diameter size may each be arbitrarily selected size, but, for as far as possible less with liquid sample to be measured and
Reduce the consideration of the aspects such as reagent loss, described pipeline and described lateral and described manifold shape turnout
The passage of capillary level, the passage of described capillary level is the most all selected to imply that on its internal diameter and ordinary meaning
The passage that the internal diameter of capillary tube is suitable.The shape of cross section of described its inner passage of capillary tube can be arbitrary
Shape, described shape of cross section is the most circular, oval, square, rectangle, bar shaped, naturally it is also possible to be
It is linear that arbitrary existence bends, and, the interior shape of described capillary tube is along with the extension of pipeline, different
The shape of cross section at position can also allow to be different shapes.Only for capillary tube one word, its technology contains
Justice is known.
Relate in structure is minute sized electrode to electrode and reference electrode, its electrode shape
To be arbitrarily selected shape, described arbitrarily selected shape such as square piece shape, rectangular patch, strip or
Round sheet etc..Described is known to itself the art-recognized meanings of vocabulary of electrode and described reference electrode
's.
Relating to several liquid pools in this case microfluidic chip structure, described liquid pool is the pond for transitional liquid storage
Shape or scrotiform structure, its shape of the inner chamber of each liquid pool may each be arbitrarily selected shape, institute
State cavity shape such as cylindrical empty cavity-like, square column type cavity-like, oblong cavity shape or spherical hollow space shape etc.
Deng.The size of described liquid pool can be arbitrarily selected size, but, in order to use liquid to be measured less as far as possible
Sample and reduction reagent loss, described liquid pool is preferably capable of the hypomegetic liquid pool mated with capillary tube.
Only with regard to itself art-recognized meanings of ultrasonic transducer one word for the professional in ultrasonic technology field
For, it is known.
Various sizes, variously-shaped ultrasonic transducer are commercially available;Commercially available miniature ultrasonic transducer units
Its size may diminish to the magnitude only calculated with millimeter.
Only with regard to its technique for fixing on general industry application solid body surface of miniature ultrasonic transducer units
For itself, for the professional in ultrasonic technology field, it it is known general technology.This case
This is not launched superfluous words.
Only with regard to naked PDMS substrate itself micro-channel mold or lithographic technique for, be open-and-shut known
Technology;Similarly, the technology of hole-opening known simple technique especially on naked PDMS substrate.This case
This is not launched superfluous words.
The industrial products market of involved higher-order of oscillation electric signal its all size of transmission cable is the most on sale.
The structure of this micro fluidic device can also include higher-order of oscillation electric signal generator;Described higher-order of oscillation electricity
Its other end of signal transmission cable can be connected with this higher-order of oscillation electric signal generator.
Itself technology of involved higher-order of oscillation electric signal generator, for the professional people in ultrasonic technology field
For Yuan, it is simple and known;Described higher-order of oscillation electric signal generator can be special to ultrasonic instrument
Industry producer customizes.
The preferred scope of its specified ultrasonic emitting power of this miniature ultrasonic transducer units be between 2 milliwatts with
Between 2000 milliwatts;The frequency of its ultrasound wave operationally launched of this miniature ultrasonic transducer units preferred
Scope is between 100KHz and 12MHz.
This case device can further include some adnexaes certainly, described adnexa such as multiple tracks electrochemical operation
Standing etc., the art-recognized meanings of described multiple tracks electrochemical workstation is known.In this case microfluidic chip structure
Each working electrode of relating to and to electrode and reference electrode etc., can be respectively via corresponding special string
Line couples with the corresponding interface of described multiple tracks electrochemical workstation.Described special getting lines crossed is being used to Jiang Gesuo
State the private cable that each the corresponding interface of electrode and described multiple tracks electrochemical workstation carries out being coupled to each other.This case
Described micro-fluidic chip in device, its structure can also include that micro-valve, the quantity of described micro-valve do not limit, root
According to being actually needed, any need position to be mounted that described micro-valve can be installed in this microfluidic chip structure;
This micro-valve one word is for the professional of micro fluidic chip technical field, and the art-recognized meanings of itself is public
Know;This micro-valve itself manufacturing technology and use technology be also known;This optional structure of micro-valve
Part.
The diameter of described working electrode can allow the suitable diameter being easily installed use being arbitrarily set,
It is however recommended to or say preferred its scope of described diameter between 0.1 micron to 2000 micron;Described
The length of working electrode can allow the length being easily installed use being arbitrarily set, it is however recommended to or
Saying preferred its scope of described length is between 1 micron to 15000 micron.
It is installed in described working electrode surface layer by spraying or point sample instrument point sample or the coating of other appropriate process
Described gold size sensitive membrane, its thicknesses of layers can allow the sample measuring liquid treated being arbitrarily set that electrically letter occurs
The thickness of number response, it is however recommended to thickness in other words conj.or perhaps preferably thickness be between 10 nanometers and 200
Between nanometer.
Described cover plate in chip structure, its material can allow to be any electrical insulating property material, such as:
Polypropylene, glass, polymethyl methacrylate, polydimethylsiloxane, etc., in order to make less chi
Very little micro-fluidic chip, such as makes the micro-fluidic core of the super-small of length only 2.0 centimetres to 3.0 centimetres
Sheet, and in the distance that this is extremely short, realize the extremely fast decay to ultrasound wave, it may be preferred to polydimethylsiloxanes
Alkane is used as cover plate.Certainly, large-sized micro-fluidic chip selects use polydimethylsiloxane to make
For described cover plate, also it is that this case technical scheme is allowed.
Be positioned at this micro-fluidic chip this sample introduction end these two liquid pools its be positioned at this terminal of this micro-fluidic chip
This remaining liquid pool between air line distance can be arbitrarily selected suitable distance as required,
But, the preferred value of this distance is between 3 centimetres and 7 centimetres.
Described cover plate and its thickness of substrate can allow the thickness being easy to assembling being arbitrarily set, it is recommended that thickness
Spending or saying preferred thickness is between 1.0 millimeters and 5.0 millimeters.Less thickness is conducive to saving material
Material.
The using method of this case micro-fluidic chip:
Propose first based on this case and this first public novel liquid stream driving principle, this case micro-fluidic chip its
Among application running, this novel liquid stream driving method determines completely without involving any additional Micropump.
This interfacial tension formed between these two ends of this micro-fluidic chip that this case is caused with described ultrasound wave
Difference, drives liquid stream to flow in the capillary channel of this four-way micro-fluidic chip, utilizes four-way electrification
Learn analytical tool respectively four kinds of aids antibodies to be detected.
Detect corresponding four kinds of aids antibodies with four kinds of acquired immune deficiency syndrome (AIDS) antigens, come with four kinds of aids antibodies
Detect corresponding four kinds of acquired immune deficiency syndrome (AIDS) antigens, similarly, can diagnosis of aids;The principle of its foundation is,
Necessarily antigen, antibody deposit in acquired immune deficiency syndrome (AIDS) patient, and mutually Reversible binding, form reversible conjugate,
Therefore, detect antibody thereby diagnosis of aids with antigen, go detect antigen and diagnose AIDS with antibody
Disease, can reach diagnostic purpose.Certainly, its corresponding electrode sensitive decorative layer of different means is in technology
Go up and differ.
The concrete detection of this case micro-fluidic chip uses step as follows:
1, in micro-pipeline, blood serum sample liquid, under described ultrasound wave drives, various aids antibodies are added
Molecule is by the Chinese mugwort of the corresponding horseradish peroxidase-labeled of gold size sensitive membrane embedding on electrode surface in each passage
Grow disease-specific antigen capture.
2, the acquired immune deficiency syndrome (AIDS) specific antigen of horseradish peroxidase-labeled and the aids antibody in blood serum sample
Form immune complex.
3, use multi-channel electrochemical analyzer, add the electron mediators such as catechol, use Amperometric
Detect the curent change that above-mentioned reaction causes, be derived from kind and the content of various analyte.
4, result is comprehensively analyzed, aids antibody is carried out comprehensive diagnos.
It is an advantage of the invention that its close position of described terminal at described micro-fluidic chip is installed micro-with attaching
Type ultrasonic transducer, utilize its low-power launched of this miniature ultrasonic transducer units, high-frequency band super
Sound wave so that without surface chemical modification this most hydrophobic its tube wall of micro-fluidic chip internal pipeline with
Test object water solution between the compatibility be significantly increased, this for sample liquid stream by providing a reality
Probability;Meanwhile, utilize its strong absorbability to ultrasound wave of polydimethylsiloxane substrate, comparing
In short distance, it is, the only several centimeters yardstick from described terminal to described sample introduction end is the shortest
Apart from interior, reaching the rapid decrement of ultrasonic intensity, thereby institute is caused at the described two ends at this micro-fluidic chip
State the difference of interfacial tension, and then, utilize the interfacial tension between these two ends difference its formed this two
Pressure gap between end, drive sample liquid stream in the capillary channel that such a is originally the most hydrophobic to
Flow in described terminal direction.By this case liquid stream drive scheme, entirely without must be to this polydimethylsiloxane material
The substrate of matter and internal pipeline thereof carry out any surface chemical modification or chemical modification, have altogether dispensed with this table
Face chemical modification or the laborious procedures of chemical modification;And altogether dispense with traditional Micropump etc
Equipment;On the other hand, the ultrasound wave of this low-power, high-frequency band, additionally it is possible to the biology in containment sample is big
Molecule absorption on this literalness naked its inner surface of pipeline of polydimethylsiloxane substrate, and then containment
The effect of swallowing up of this polydimethylsiloxane substrate the most described biomacromolecule of its body;Described antigen, antibody
And the Reversible binding thing of antigen and antibody is the most all belonging to the type of described biomacromolecule;Due to institute
Adsorption and the described effect of swallowing up stated are contained effectively, and therefore, dependence test result will more
Practical situation can be reflected objectively;This low-power, the effect of high-frequency band ultrasound wave, the most also include promoting
Become between antigen, antibody Reversible binding reaction quickly reach, this make dependence test operation can with than
Speed completes faster.
Due to be made without the surface chemical modification for this polydimethylsiloxane its relevant surfaces of substrate or
Chemical modification operates, and therefore, this surface chemical modification layer or chemically modified layer not need to exist,
So, its body phase interior polymeric thing molecule of this polydimethylsiloxane substrate the most automatically diffuses to the surface, moves
Move its damaging influence to described surface chemical modification layer or chemically modified layer caused not exist
?.
The technical scheme of this case dissolved totally address above with polydimethylsiloxane substrate its application
Relevant a series of technical barriers.Based on this case scheme, the polydimethyl siloxane material that this kind is the most cheap
The most likely prepare at this micro-fluidic chip, produce, application etc. field plays bigger effect.
Accompanying drawing explanation
Fig. 1 is the schematic diagram of this micro fluidic device embodiment chip part of this case structure, it is shown that should
Chip internal pipeline under the depression angle of example structure and each electrode and the configuration of each relevant liquid pool, this figure
In do not depict described miniature ultrasonic transducer units and other described adnexa.
Fig. 2 is its rough outside side view of this micro fluidic device of this case.
In figure, 1,2,10 is the liquid pool that three installation positions are different respectively, and 3 is manifold shape turnout, 4,7,
11,14 are installation position difference but four laterals forming UNICOM in parallel structure parallel with one another respectively, 5
Be the specific antigenic substance in its top layer gold size sensitive membrane structure being installed in lateral 4 be acquired immune deficiency syndrome (AIDS)
The working electrode of specific antigen p24,6 is its top layer gold size sensitive membrane knot being installed in lateral 7
Specific antigenic substance in structure is the working electrode of acquired immune deficiency syndrome (AIDS) specific antigen gp41, and 12 is to be installed in point
The specific antigenic substance in its top layer gold size sensitive membrane structure in branch pipe(tube) 11 is that acquired immune deficiency syndrome (AIDS) specificity resists
The working electrode of former gp120,13 is in its top layer gold size sensitive membrane structure being installed in lateral 14
The working electrode that specific antigenic substance is acquired immune deficiency syndrome (AIDS) specific antigen gp36,8 is to electrode, 9 be ginseng
Ratio electrode, 15 is the substrate of polydimethylsiloxane material, and 16 is cover plate, and 17 is higher-order of oscillation electric signal
Transmission cable, 18 is miniature ultrasonic transducer units, and 19 is the described sample introduction end of this micro-fluidic chip, and 20 are
The described terminal of this micro-fluidic chip;Arrow in legend indicate this micro-fluidic chip its at actual motion
Time, driven by pressure at two ends difference, the flow direction of its sample liquid stream.
Detailed description of the invention
In this embodiment of this case that Fig. 1 and Fig. 2 is shown, the structure of this micro fluidic device includes multichannel
Micro-fluidic chip, the structure of this micro-fluidic chip includes bonded to each other being installed in substrate 15 together and cover plate
16, described substrate 15 and cover plate 16 are plate object or tablet, this substrate 15 towards this cover plate 16
That face contain the channel structure formed via mould pressing process or etching technics, this substrate 15 possibly together with this
Channel structure is connected and pierces being formed via mould pressing process, etching technics or simple drilling technology of this substrate
Window structure, this substrate 15 being installed together bonded to each other has jointly been built into this cover plate 16 and has contained
Pipeline configuration and the micro-fluidic chip of liquid pool structure being attached thereto, described liquid pool be liquid pool 1, liquid pool 2,
Liquid pool 10, the locations of structures of this pipeline is positioned at the interface zone that this substrate 15 is bonded to each other with this cover plate 16,
Its side of this window is blocked by this cover plate 16 and opposite side is open, and the locations of structures of this window is exactly described liquid pool
1, liquid pool 2, the locations of structures of liquid pool 10, the quantity of described liquid pool is three, and two liquid pools therein are i.e.
Liquid pool 1 and its locations of structures of liquid pool 2 are positioned at the sample introduction end 19 of this micro-fluidic chip, and a remaining liquid pool is i.e.
The end of its chip liquid flowing when its locations of structures of liquid pool 10 is positioned at the test of this micro-fluidic chip actual sample introduction
End 20, this terminal 20 is located remotely from each other with this sample introduction end 19, and one end of this pipeline is mutual via being similarly positioned in this
Laminating interface zone manifold shape turnout 3 respectively with this liquid pool 1 and this liquid pool 2 being positioned at sample introduction end 19
UNICOM, this remaining liquid pool of the other end of this pipeline and the described terminal 20 being positioned at this micro-fluidic chip
I.e. liquid pool 10 UNICOM, and, the working electrode that is sequentially respectively installed in this pipeline on diverse location and right
Electrode 8 and reference electrode 9, described working electrode is by conductive electrode and is attached to this conductive electrode
On embedded acquired immune deficiency syndrome (AIDS) specific antigen gold size sensitive membrane constitute, the structure of this pipeline is parallel construction,
The described pipeline in parallel construction is made up of four lateral parallel connections, and the quantity of described working electrode is four,
The installation position of these four working electrodes lays respectively in described four laterals, and these four working electrodes divide
It not working electrode 5, working electrode 6, working electrode 12, working electrode 13, and, these four work
Specific antigen in its top layer gold size sensitive membrane structure of electrode is can be specific binding to aids antibody respectively
Four kinds of acquired immune deficiency syndrome (AIDS) antigenic substances, these four kinds of antigenic substances be respectively acquired immune deficiency syndrome (AIDS) specific antigen p24, gp41,
Gp120 and gp36, from the point of view of specifically launching, 5 is that its top layer gold size being installed in lateral 4 is sensitive
Specific antigenic substance in membrane structure is the working electrode of acquired immune deficiency syndrome (AIDS) specific antigen p24, and 6 is to be installed in
The specific antigenic substance in its top layer gold size sensitive membrane structure in lateral 7 is that acquired immune deficiency syndrome (AIDS) specificity resists
The working electrode of former gp41,12 is in its top layer gold size sensitive membrane structure being installed in lateral 11
The working electrode that specific antigenic substance is acquired immune deficiency syndrome (AIDS) specific antigen gp120,13 is to be installed in branch
The specific antigenic substance in its top layer gold size sensitive membrane structure in pipeline 14 is acquired immune deficiency syndrome (AIDS) specific antigen
The working electrode of gp36, described working electrode 5, working electrode 6, working electrode 12, working electrode 13
Its material is gold material or thermal decomposition conducting polymer material, described working electrode 5, working electrode 6, work
Make electrode 12, its pattern of working electrode 13 presents lamellar or thread, it is important that, this its material of substrate 15 is
Polydimethylsiloxane material, this its surface of substrate 15 is the surface of primary form, the table of this primary form
Face its be intended to refer to not through this material former of any surface chemical modification or any surface chemical modification
The surface of raw form, the structure of this micro fluidic device also includes miniature ultrasonic transducer units 18, and, high frequency
Vibration electric signal transmission cable 17, one end of this higher-order of oscillation electric signal transmission cable 17 and this miniature ultrasonic
Wave transducer 18 links together, and this miniature ultrasonic transducer units 18 is installed in this micro-fluidic chip with attaching
Cover plate 16 or the position of neighbouring described terminal 20 of substrate 15;Its master of this miniature ultrasonic transducer units 18
Wanting function is when the actual sample introduction of micro-fluidic chip is tested, and utilizes its ultrasound wave launched molten to reduce sample
Interfacial tension between the inwall of liquid and described pipeline so that it is can be compatible, and, utilize described sample introduction end
Distance difference between 19 and described terminal 20 and this miniature ultrasonic transducer units 18 installation position and
Difference on its ultrasonic intensity experienced, its interfacial tension of sample introduction end 19 and institute described in induced synthesis
State the difference between its interfacial tension of terminal 20, these two ends of this micro-fluidic chip i.e. sample introduction end 19 and terminal
Interfacial tension difference between 20 can at these two ends of this micro-fluidic chip i.e. sample introduction end 19 and terminal 20 it
Between formed pressure gap, this pressure gap can drive sample solution to flow to described terminal 20 direction;This is micro-
Its function of type ultrasonic transducer 18 also includes checking life contained in sample with its ultrasound wave launched
Its absorption on described inner surface of pipeline of thing macromole, and then check the base of this polydimethylsiloxane material
Its body of sheet 15 is relative to the effect of swallowing up of this biomacromolecule;The substrate 15 of described polydimethylsiloxane material
Its function includes and cover plate 16 and working electrode 5, working electrode 6, working electrode 12, working electrode 13
And electrode 8 and reference electrode 9 are together built this micro-fluidic chip, softness also has this elastic poly dimethyl
Its function of the substrate of siloxanes material also includes, with the character of its absorption strong to ultrasound wave, carrying out ultrasound wave
Strong absorb, and thereby within limited short distance between this terminal of this micro-fluidic chip to this sample introduction end
Realize the rapid decrement of ultrasonic intensity.
Arrow in legend indicate this micro-fluidic chip its when actual motion, driven by pressure at two ends difference
Dynamic, the flow direction of its sample liquid stream.
Fig. 1 is depicted without miniature ultrasonic transducer units 18 and higher-order of oscillation electric signal transmission cable 17;
Further, Fig. 1 and Fig. 2 is all depicted without described higher-order of oscillation electric signal generator and multiple tracks electrochemical operation
The associate member such as stand.
Involved miniature ultrasonic transducer units 18 is commercially available;Can also customize to ultrasonic transducer producer.
Involved higher-order of oscillation electric signal transmission cable 17 is commercially available;Can also be to ultrasonic transducer producer
Customization.
Involved higher-order of oscillation electric signal generator market has the product close to needs commercially available;Can also be to phase shutout
Family's customization.
Each working electrode in this example structure and can be respectively via respectively acting on one's own to electrode and reference electrode
Cable or say get lines crossed respectively with the corresponding cable interface of the multiple tracks electrochemical workstation as adnexa or say string
Line interface couples.
This case device is to detect multiple corresponding aids antibody with multiple acquired immune deficiency syndrome (AIDS) antigen, thereby diagnoses
Acquired immune deficiency syndrome (AIDS).
Claims (10)
- Transmit the cheap micro fluidic device of AIDS diagnosis of liquid stream, the structure of this micro fluidic device the most in a new way Including multichannel micro-fluidic chip, the structure of this micro-fluidic chip includes the substrate being installed in together bonded to each other And cover plate, described substrate and cover plate be plate object or tablet, that face towards this cover plate of this substrate Containing the channel structure formed via mould pressing process or etching technics, this substrate possibly together with this channel structure phase Connect and pierce the window structure formed via mould pressing process, etching technics or simple drilling technology of this substrate, This substrate being installed together bonded to each other and this cover plate have been built into jointly containing pipeline configuration and phase therewith The micro-fluidic chip of liquid pool structure even, it is bonded to each other with this cover plate that the locations of structures of this pipeline is positioned at this substrate Interface zone, its side of this window is blocked by this cover plate and opposite side is open, and the locations of structures of this window is just Being the locations of structures of described liquid pool, the quantity of described liquid pool is three, two its locations of structures of liquid pool therein Being positioned at the sample introduction end of this micro-fluidic chip, it is real that remaining its locations of structures of liquid pool is positioned at this micro-fluidic chip The terminal of liquid flowing in its chip during the sample introduction test of border, this terminal is located remotely from each other with this sample introduction end, this pipeline One end via be similarly positioned in the manifold shape turnout of this interface zone bonded to each other respectively be positioned at sample introduction end This Liang Ge liquid pool UNICOM, the other end of this pipeline is remaining with this of described terminal being positioned at this micro-fluidic chip One liquid pool UNICOM, and, the working electrode that is sequentially respectively installed in this pipeline on diverse location and right Electrode and reference electrode, described working electrode is by conductive electrode and is attached on this conductive electrode The gold size sensitive membrane having embedded acquired immune deficiency syndrome (AIDS) specific antigen is constituted, and the structure of this pipeline is parallel construction, described Pipeline in parallel construction is made up of four lateral parallel connections, and the quantity of described working electrode is four, should The installation position of four working electrodes lays respectively in described four laterals, and, these four work electricity The extremely specific antigen in top layer gold size sensitive membrane structure respectively can be specific binding to aids antibody Four kinds of acquired immune deficiency syndrome (AIDS) antigenic substances, these four kinds of antigenic substances be respectively acquired immune deficiency syndrome (AIDS) specific antigen p24, gp41, Gp120 and gp36, its material of described working electrode is gold material or thermal decomposition conducting polymer material, institute State its pattern of working electrode and present lamellar or thread, it is characterised in that its material of this substrate is poly dimethyl silicon Oxygen alkane material, its surface of this substrate is the surface of primary form, the surface of this primary form its be intended to refer to Not through the surface of primary form of this material of any surface chemical modification or any surface chemical modification, The structure of this micro fluidic device also includes miniature ultrasonic transducer units, and, higher-order of oscillation electric signal transmission electricity Cable, one end of this higher-order of oscillation electric signal transmission cable links together with this miniature ultrasonic transducer units, should Miniature ultrasonic transducer units is installed in the cover plate of this micro-fluidic chip or the neighbouring described terminal of substrate with attaching Position;Its major function of this miniature ultrasonic transducer units is when the actual sample introduction of micro-fluidic chip is tested, and utilizes Its ultrasound wave launched is to reduce the interfacial tension between the inwall of sample solution and described pipeline so that it is energy Enough compatible, and, utilize described sample introduction end and described terminal and this miniature ultrasonic transducer units installation position Between distance difference and difference on its ultrasonic intensity experienced, sample introduction end described in induced synthesis Difference between its interfacial tension and its interfacial tension of described terminal, the boundary between these two ends of this micro-fluidic chip Surface tension difference can form pressure gap between these two ends of this micro-fluidic chip, and this pressure gap can drive Sample solution is to described end flow;Its function of this miniature ultrasonic transducer units also included with surpassing that it is launched Sound wave checks its absorption on described inner surface of pipeline of biomacromolecule contained in sample, and then checks Its body of the substrate of this polydimethylsiloxane material is relative to the effect of swallowing up of this biomacromolecule;Described poly-diformazan Its function of the substrate of radical siloxane material includes and cover plate and working electrode and to electrode and reference electrode one isomorphism Building this micro-fluidic chip, soft and this polydimethylsiloxane material of having elasticity its function of substrate also includes With the character of its absorption strong to ultrasound wave, ultrasound wave is absorbed strongly, and thereby at this micro-fluidic core This terminal of sheet is to the rapid decrement realizing ultrasonic intensity within the limited short distance between this sample introduction end.
- The cheap micro-fluidic dress of AIDS diagnosis transmitting liquid stream in a new way the most according to claim 1 Put, it is characterised in that described pipeline and described lateral and described manifold shape turnout are capillary tube Passage.
- The cheap micro-fluidic dress of AIDS diagnosis transmitting liquid stream in a new way the most according to claim 1 Put, it is characterised in that described thermal decomposition conducting polymer is through anoxybiotic heat by polyimides or polyacrylonitrile The conductive material formed after reason.
- The cheap micro-fluidic dress of AIDS diagnosis transmitting liquid stream in a new way the most according to claim 1 Put, it is characterised in that the width of described working electrode or diameter between 0.1 micron to 2000 micron, And, the length of described working electrode is between 1 micron to 15000 micron.
- The cheap micro-fluidic dress of AIDS diagnosis transmitting liquid stream in a new way the most according to claim 1 Put, it is characterised in that the thickness of described gold size sensitive membrane is in 10 nanometers and 200 between rice.
- The cheap micro-fluidic dress of AIDS diagnosis transmitting liquid stream in a new way the most according to claim 1 Put, it is characterised in that its material of described cover plate in structure is polydimethylsiloxane material.
- The cheap micro-fluidic dress of AIDS diagnosis transmitting liquid stream in a new way the most according to claim 1 Put, it is characterised in that be positioned at this micro-fluidic chip this sample introduction end these two liquid pools its be positioned at this miniflow Air line distance between this remaining liquid pool of control this terminal of chip is between 3 centimetres and 7 centimetres.
- The cheap micro-fluidic dress of AIDS diagnosis transmitting liquid stream in a new way the most according to claim 1 Put, it is characterised in that described cover plate in structure and its thickness of substrate all between 1.0 millimeters with 5.0 millimeters Between.
- The cheap micro-fluidic dress of AIDS diagnosis transmitting liquid stream in a new way the most according to claim 1 Put, it is characterised in that the structure of this micro fluidic device also includes higher-order of oscillation electric signal generator, described height Frequency vibration is swung its other end of electric signal transmission cable and is connected with this higher-order of oscillation electric signal generator.
- The cheap micro-fluidic dress of AIDS diagnosis transmitting liquid stream in a new way the most according to claim 1 Put, it is characterised in that its specified ultrasonic emitting power of this miniature ultrasonic transducer units is between 2 milliwatts and 2000 Between milliwatt, the frequency of its ultrasound wave operationally launched of this miniature ultrasonic transducer units be between Between 100KHz and 12MHz.
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Citations (2)
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CN101661038A (en) * | 2009-07-12 | 2010-03-03 | 宁波大学 | Special multi-channel micro-flow controller used in syphilis diagnosis and using low-priced conducting material |
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CN101661038A (en) * | 2009-07-12 | 2010-03-03 | 宁波大学 | Special multi-channel micro-flow controller used in syphilis diagnosis and using low-priced conducting material |
CN102645429A (en) * | 2012-04-23 | 2012-08-22 | 宁波大学 | Self-cleaning and vibration wave energy digestion link containing electrogenerated chemiluminescence analyzing and detecting device |
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