A kind of method of ammonia level in device for diagnosing human body helicobacter pylori infections and detection human exhaled breath
Technical field
The present invention relates to ammonia in a kind of device diagnosing human body helicobacter pylori infections and detection human exhaled breath
The method of content;Being specifically related to one utilizes human body to take common urea electuary, by carbamide and human body pylorus spiral shell
Bacillus mark urease effect release ammonia, then with the ammonia in ammonia trapping agent capture sample, use ion color
The device and method of the fixing ammonia of spectral method detection;By ammonia level in human exhaled breath is diagnosed human body pylorus
Pylori (Hp) infection degree, belongs to medical detection technology.
Background technology
Helicobacter pylori (being called for short Hp) is one of the most modal pathogen, the sense in population of China
Dye rate is up to 60%, and annual new infeetioa case is more than 12,000,000 people.The duodenum of the most about more than 90% is burst
Infections, the gastric ulcer of more than 70%, the Patients with Chronic Gastritis of more than 60% exist Hp to be infected, World Health Organization (WHO)
It is defined as the first kind carcinogen of gastric cancer.
At present, the method system that existing detection Hp infects mainly has gastroscope detection system, immunology detection body
System, polymerase chain reaction detection system, saliva method detection system, urease detection system,13C/14C carbamide
Breath test detection system etc..Above system has or brings misery or detection accuracy, ageing to person under inspection
The defects such as poor or toxic side effect.
Summary of the invention
In order to make up the deficiency having diagnosed the method system that Hp infects, it is an object of the invention to be to provide one
Kind utilize the device that unmarked urea breath test coupled ion chromatograph detects, this device can more accurately, higher
Diagnosis human body Hp in effect ground infects, and while reducing medicament production cost, eliminates conventional medicament toxic and side effects
Hidden danger.
Ammonia level in a kind of detection human exhaled breath simple and quick, accurate of offer is provided
Method, thus realize sensitive, quick, specific detection helicobacter pylori infections degree.
In order to realize above-mentioned technical purpose, the invention provides a kind of for diagnosing human body helicobacter pylori infections
Device, this device includes breath diagnosis test kit and ion chromatograph;It is provided with in described breath diagnosis test kit
Medicament pond I and medicament pond II;Described medicament pond I and medicament pond II is respectively provided with carbamide electuary and ammonia trapping agent.
Preferably scheme, carbamide electuary comprises without isotope-labeled urea components.
Preferably scheme, ammonia trapping agent includes at least HNO3、H3PO4、HCl、H2SO4, in TGA
At least one acid composition.Ammonia trapping agent can be HNO3、H3PO4、HCl、H2SO4, in TGA
At least one, or be HNO3、H3PO4、HCl、H2SO4, at least one is weak with other in TGA
The combination of acid.
Preferably scheme, test kit also includes sampler bag.
More preferably scheme, sampler bag is made up of polyvinyl-fluoride material, and sampler bag is provided with supporting threaded cap.
The device for diagnosing human body helicobacter pylori infections of the present invention.Main body includes sampler bag, breath diagnosis
Test kit and ion chromatograph.Breath diagnosis test kit use common rectangle, square, circle or they
The one of variant or combination, material is selected from wooden, papery, irony or it is with the entity of coat.Respectively
Medicament pond I and medicament pond II equipped with common urea electuary and equipped with ammonia trapping agent are placed in breath diagnosis test kit
In.Sampler bag volume 250mL, sampler bag uses polyvinyl-fluoride complex material to make, and has supporting
Threaded cap.During detection, first employing sampler bag collection human exhaled breath is as blank, then takes to human body
Carbamide electuary, after a period of time, collects human exhaled breath by sampler bag, then by human exhaled breath by ammonia trapping agent,
Ammonia is converted ammonium salt, is accurately detected the content of ammonium salt by ion chromatograph.
Present invention also offers and a kind of detect the method for ammonia level in human exhaled breath, the method includes following step
Rapid:
1) human exhaled breath is collected by sampler bag;
2) human exhaled breath that collection bag is collected is incorporated in breath diagnosis test kit;Described test kit includes
One medicament pond II, described medicament pond II are built with ammonia trapping agent, described ammonia trapping agent and the ammonia in human exhaled breath
Gas generation chemical reaction generates ammonium salt;
3) amounts of ammonium salt generated by ion chromatograph detection reaction, is i.e. calculated ammonia in human exhaled breath and contains
Amount.
Preferably scheme, ammonia trapping agent includes at least HNO3、H3PO4、HCl、H2SO4, in TGA
At least one acid composition.
Preferably scheme, also includes a medicament pond I in breath diagnosis test kit;Described medicament pond I built with
The carbamide electuary of ammonia is discharged for acting on helicobacter pylori mark urease.
More preferably scheme, described carbamide electuary comprises without isotope-labeled urea components.
The human exhaled breath of the present invention includes the expiration of release in human body normal sensation in the mouth nose.
Assembly of the invention for diagnosing the principle of human body helicobacter pylori infections is: carbamide and human body helicobacter pylorus
Bacterium mark urease effect release ammonia, then capture the ammonia of release with ammonia trapping agent and realize the fixing life of ammonia
Become ammonium salt, finally use ion chromatographic method detection ammonium salt, such that it is able to draw human body helicobacter pylori infections journey
Degree.
For prior art, the beneficial effect that technical scheme is brought:
(1) assembly of the invention is combined with the chromatography of ions by urea breath test, it is possible to quick and precisely
Measure ammonia level in human exhaled breath, it is achieved thereby that simple and quick, safety and precise ground diagnosing helicobacter pylori sense
The degree of dye.
(2) assembly of the invention uses the common urea reagent of No Parity element labelling, little to human toxicity, right
Human body stimulates little, it is easy to accept.
(3) assembly of the invention is by the collection to expiration, and uses ammonia trapping agent to fix, it is possible to quickly accurate
Really determine the content of ammonia in expiration by chromatography of ions, and then realize sensitive, quick, specific detection pylorus
Pylori (Hp) infection degree.
(4) medicament of assembly of the invention and employing is all existing routine, reduces testing cost.
(5) in the detection human exhaled breath of the present invention, the method for ammonia level can realize based on assembly of the invention,
Simply, quickly, can sensitive, quick, specific detection helicobacter pylori infections degree.
Accompanying drawing explanation
[Fig. 1] represents the chromatogram of ammonia in the blank sample that experimenter 1 breathes out;
[Fig. 2] represents the chromatogram of ammonia in the sample that experimenter 1 breathes out;
[Fig. 3] represents the chromatogram of ammonia in the blank sample that experimenter 2 breathes out;
[Fig. 4] represents the chromatogram of ammonia in the sample that experimenter 2 breathes out;
[Fig. 5] represents the chromatogram of ammonia in the blank sample that experimenter 3 breathes out;
[Fig. 6] represents the chromatogram of ammonia in the sample that experimenter 3 breathes out;
[Fig. 7] represents the chromatogram of ammonia in the blank sample that experimenter 4 breathes out;
[Fig. 8] represents the chromatogram of ammonia in the sample that experimenter 4 breathes out;
[Fig. 9] represents the chromatogram of ammonia in the blank sample that experimenter 5 breathes out;
[Figure 10] represents the chromatogram of ammonia in the sample that experimenter 5 breathes out.
Detailed description of the invention
Following example are intended to further illustrate present invention rather than limit the protection of the claims in the present invention
Scope.
Embodiment 1
1, detection object: experimenter 1, man, 41 years old, warp13C-urea breath test is diagnosed as the positive.
2, detection method:
(1), require experimenter on an empty stomach or fasting in more than two hours in advance.
(2), experimenter exhales, and entrance sampler bag is the most full is labeled as blank sample.
(3) test kit warm boiled water common urea medicament, is taken.
(4), after 30min, experimenter again exhales and is labeled as sample into sampler bag to full.
(5), the gas in the sampler bag being marked with blank sample and sample is passed through ammonia trapping agent, and uses ion
Ammonia in chromatograph detection characteristics of contaminated respiratory droplets gas.
(6), experimenter's Helicobacter pylori infection is diagnosed further by the ammonia level in expired gas.
3, testing result: testing result such as table 1.
The chromatograph detection data of ammonia in the blank sample of table 1 experimenter 1 exhalation and sample
4 conclusions:
As can be seen from Table 1, standard curve method can accurately calculate the content of ammonia during experimenter exhales, and take medicine
Before in rear expiration, the content of ammonia is much larger than taking medicine.
Embodiment 2
1, detection object: experimenter 2, man, 35 years old, warp13C-urea breath test is diagnosed as feminine gender.
2, detection method: the detection method of the present embodiment is identical with a upper embodiment, obtains its detection
Result such as table 2:
The chromatograph detection data of ammonia in the blank sample of table 2 experimenter 2 exhalation and sample
3 conclusions: as can be seen from Table 2, by standard curve method accurately calculate experimenter take medicine front and back exhale in ammonia
Content essentially identical, and be positioned at reduced levels.
Embodiment 3
1, detection object: experimenter 3, man, 26 years old, warp13C-urea breath test is diagnosed as feminine gender.
2, detection method: the detection method of the present embodiment is identical with a upper embodiment, obtains its detection
Result such as table 3:
The chromatograph detection data of ammonia in the blank sample of table 3 experimenter 3 exhalation and sample
3 conclusions: as can be seen from Table 3, by standard curve method accurately calculate experimenter take medicine front and back exhale in ammonia
Content essentially identical, and be positioned at reduced levels.
Embodiment 4
1, detection object: experimenter 4, man, 43 years old, warp13C-urea breath test is diagnosed as feminine gender.
2, detection method: the detection method of the present embodiment is identical with a upper embodiment, obtains its detection
Result such as table 4:
The chromatograph detection data of ammonia in the blank sample of table 4 experimenter 4 exhalation and sample
3 conclusions: as can be seen from Table 4, by standard curve method accurately calculate experimenter take medicine front and back exhale in ammonia
Content essentially identical, and be positioned at reduced levels.
Embodiment 5
1, detection object: experimenter 5, female, 30 years old, warp13C-urea breath test is diagnosed as the positive.
2, detection method: the detection method of the present embodiment is identical with a upper embodiment, obtains its detection
Result such as table 5:
The chromatograph detection data of ammonia in the blank sample of table 5 experimenter 5 exhalation and sample
3 conclusions: as can be seen from Table 5, are accurately calculated the content clothes of ammonia during experimenter exhales by standard curve method
Before relatively larger than taking medicine after medicine.
Experiment shows, use the present invention detection system, can not only Accurate Diagnosis human body helicobacter pylori infections with
No, also can accurately detect the degree of helicobacter pylori infections.Ammonia content in human exhaled breath can be made tentatively simultaneously
Statistics, ammonia content scope 0~2.0ppm in normal person's expiration, the most densely distributed interval is 1.0~1.5
Ppm scope;Ammonia content scope 1.7~10ppm in infected with Helicobacter pylori expiration, the most densely distributed
Interval is 2.2~6.0ppm scopes.There is provided reliable basis for treatment helicobacter pylori infections, can fully meet and examine
The application requirement of disconnected human body helicobacter pylori infections.