CN105878612A - Bee venom essence and preparation method thereof - Google Patents
Bee venom essence and preparation method thereof Download PDFInfo
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- CN105878612A CN105878612A CN201410558596.7A CN201410558596A CN105878612A CN 105878612 A CN105878612 A CN 105878612A CN 201410558596 A CN201410558596 A CN 201410558596A CN 105878612 A CN105878612 A CN 105878612A
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Abstract
The invention relates to a bee venom essence. The essence comprises the following components: apitoxin, propolis, notoginseng, frankincense, safflower, prepared aconite main roots, prepared wild aconite roots, homalomena roots, zanthoxylum husks, common clubmoss herbs, garden balsam stems, borneol, camphor, menthol, capsicine, chlorhexidine acetate, cinnamon oil, turpentine, and wintergreen oil. The invention also relates to a preparation method of a bee venom essence. The preparation method comprises the following steps: S1: a bee venom taking device is prepared; S2: bee venom is collected; S3: purification of bee venom; S4: a propolis solution is prepared for standby by completely dissolving propolis with polyethylene glycol; S5: Chinese herbal medicines are uniformly mixed according to a formula proportion; S6: bee venom is added into a proper amount of Tween 80 and is mixed with the propolis solution and the traditional Chinese medicine concentrate for standby; S7: wintergreen oil and other oil substances are added into the mixed liquor in order to obtain mixed liquor which is the bee venom essence finished product. The method has the beneficial effects that the bee venom essence does not induce allergy and stimulation, does not have toxic and side effects, and have good effects; and the preparation method of the bee venom essence has a simple process and a high efficiency.
Description
Technical field
The present invention relates to a kind of Venenum apis essence and preparation method thereof.
Background technology
The history of melissotherapy: before more than 1700 year, ancient Roman's physician's Galen describes Venenum apis pain relieving etc.
Multiple use.China is with a long history as traditional medicine using bee product, utilizes Apis to bite thorn treatment of arthritis
And a lot of difficult and complicated illness such as neuralgia are the widest at folklore.Traditional honeybee acupuncture honeybee therapy alive is to utilize to live
Apis directly stings to sting and carries out curing the disease on the relevant acupuncture point of patient.Due to the most purified place of full Venenum apis
Reason, Venenum apis includes after anaphylactogen enzyme material enters human body easily occurs that allergy, redness, pain are even drawn
Playing shock, the acupuncture point adding thorn of stinging is difficult to grasp, and catches the defects such as Apis is subject to seasonal restrictions of living, seriously
The popularization hindering honeybee acupuncture melissotherapy alive.
Summary of the invention
In view of the above-mentioned problems in the prior art, present invention is primarily targeted at solution prior art
Defect, the present invention provide a kind of without allergy, non-stimulated, have no side effect and honeybee that therapeutic effect is good
The preparation method of the malicious smart and a kind of Venenum apis that technique is simple and production efficiency is high essence.
The invention provides a kind of Venenum apis essence, including following components: melittin, propolis, Radix Notoginseng, Olibanum,
Flos Carthami, Radix Aconiti Preparata, Radix Aconiti Kusnezoffii Preparata, Rhizoma Homalomenae, Fructus Zanthoxyli, Herba Lycopodii, Herba speranskiae tuberculatae, Borneolum Syntheticum, Camphora, thin
Lotus brain, capsaicin, chlorhexidine acetate, Oleum Cinnamomi, Oleum Terebinthinae and wintergreen oil, wherein, described melittin
Quality be 0.10～0.20g, the quality of described propolis is 2～4g, the quality of described Radix Notoginseng be 2～
4g, the quality of described Olibanum is 9～11g, and the quality of described Flos Carthami is 9～11g, described Radix Aconiti Preparata
Quality is 5～7g, and the quality of described Radix Aconiti Kusnezoffii Preparata is 5～7g, and the quality of described Rhizoma Homalomenae is 8～12g,
The quality of described Fructus Zanthoxyli is 8～12g, and the quality of described Herba Lycopodii is 35～45g, the matter of described Herba speranskiae tuberculatae
Amount is 25～35g, and the quality of described Borneolum Syntheticum is 1～5g, and the quality of described Camphora is 1～4g, described
The quality of Mentholum is 0.2～0.8g, and the quality of described capsaicin is 1～4g, described chlorhexidine acetate
Quality be 2～5g, the quality of described Oleum Cinnamomi is 8～13g, described terebinthine quality be 35～
45g, the quality of described wintergreen oil is 90～110g.
Optionally, the quality of described melittin is 0.15g, and the quality of described propolis is 3g, described three
The quality of seven is 3g, and the quality of described Olibanum is 10g, and the quality of described Flos Carthami is 10g, described river processed
The quality of crow is 6g, and the quality of described Radix Aconiti Kusnezoffii Preparata is 6g, and the quality of described Rhizoma Homalomenae is 10g, described
The quality of Fructus Zanthoxyli is 10g, and the quality of described Herba Lycopodii is 40g, and the quality of described Herba speranskiae tuberculatae is 30g,
The quality of described Borneolum Syntheticum is 3g, and the quality of described Camphora is 2g, and the quality of described Mentholum is 0.5g,
The quality of described capsaicin is 2g, and the quality of described chlorhexidine acetate is 3g, the quality of described Oleum Cinnamomi
For 10g, described terebinthine quality is 40g, and the quality of described wintergreen oil is 100g.
Optionally, the quality of described melittin is 0.18g, and the quality of described propolis is 3.5g, described
The quality of Radix Notoginseng is 2.5g, and the quality of described Olibanum is 10.5g, and the quality of described Flos Carthami is 9.5g,
The quality of described Radix Aconiti Preparata is 5.5g, and the quality of described Radix Aconiti Kusnezoffii Preparata is 6.5g, the quality of described Rhizoma Homalomenae
For 11g, the quality of described Fructus Zanthoxyli is 9g, and the quality of described Herba Lycopodii is 42g, the matter of described Herba speranskiae tuberculatae
Amount is 28g, and the quality of described Borneolum Syntheticum is 4g, and the quality of described Camphora is 3g, the matter of described Mentholum
Amount is 0.65g, and the quality of described capsaicin is 2.5g, and the quality of described chlorhexidine acetate is 4g, institute
The quality stating Oleum Cinnamomi is 12g, and described terebinthine quality is 42g, and the quality of described wintergreen oil is 95g.
The present invention also provides for the preparation method of a kind of Venenum apis essence, comprises the following steps:
Prepared by S1, Venenum apis venon extractor: glass plate one piece, sieve-like wire netting, plastic sheeting one, direct current
One, transformator, supply socket, wire netting one side is covered with plastic sheeting, plastic sheeting is towards glass plate side
Fix to distance 0.5cm wooden frame；Transformer frequency response is connected with wire netting；
S2, collection Venenum apis: collecting Venenum apis and select the autumn in summer to replace season, now Apis Venenum apis content is high, not
Gathering venom during cloudy day blowing and raining, sky temperature is not less than 20 DEG C, and when taking poison, operator put on protection again
Clothes, protective footwear, wear protective mast；Venon extractor is placed on beehive mouth, treats that Apis climbs full wire netting and divides
Cloth is uniform, turns on the power switch, and voltage progressively heightens 30 volts, is energized 15 seconds, has a power failure 5 seconds, as
This circulation operation 15 minutes, Apis tailpin punctures plastic sheeting, Venenum apis is arranged on a glass,
Close power supply after 15 minutes, stop 10～15 minutes, after Apis is emotionally stable, take glass plate away；
S3, the purification of Venenum apis: include that magazins' layout separates with anaphylactogen, wherein, described magazins' layout is
By the Venenum apis vinegar solution containing impurity with in the pure water of 5～10 times, filter with middling speed qualitative filter paper, remove
Remove the insoluble matter impurity such as dust, Venenum apis solution adds the acetone of 2 times, makes Venenum apis Precipitation, point
Full Venenum apis is obtained after separating out moisture drying；Described anaphylactogen is separated into ultrafiltration and decomposes histamine and phospholipase A2,
Use acetate fiber and cellulose nitrate mixing filter membrane, filter Venenum apis solution and histamine is removed, then in Venenum apis
Solution instills qs glycerin, phospholipase A2 is resolved into phospholipid and fatty acid；
It is standby that S4: propolis Polyethylene Glycol is completely dissolved preparation propolis liquid；
S5: mixed according to formula proportion by Chinese herbal medicine, decocts for 2 times with moisture, merges decocting liquid, add ice
Sheet, Camphora, Mentholum, capsaicin and chlorhexidine acetate 200 mesh filtering and concentrating to 1000ml；
S6: Venenum apis is added Tween 80 in right amount, mix with propolis liquid, propolis mixed liquor and traditional Chinese drug inspissation
Liquid mixing for standby use；
S7: above-mentioned mixed liquor is added in wintergreen oil mixed liquor Venenum apis essence finished product.
Optionally, when collecting Venenum apis in described step S1, wear masks protection, cleans with 2 times of white vinegars
Glass plate, makes Venenum apis avoid with air contact and aoxidizes, Venenum apis solution is loaded in vial and stores.
The present invention has the following advantages and beneficial effect: a kind of Venenum apis essence of present invention offer and preparation side thereof
Method, combines with folkd therapy, melissotherapy, scrape therapy according to tcm family tradition secret recipe, fortune
Purifying with medicine and form with synthetic technology scientific composition, this Venenum apis is smart by the anaphylactogen purification & isolation of Venenum apis,
Allergy is not non-stimulated, have no side effect, and is efficient high-tech biological product；Meanwhile, this kind of Venenum apis essence
Appearance, filled up safety of China and used Venenum apis, the sky of propolis and natural Chinese medicine synthesis quintessence oil liniment product
In vain, indicate that China enters brand-new rank in fields such as rheumatic osteopathia, tcm health preserving, scrape therapy physical therapys
Section；Meanwhile, the preparation method technique of this Venenum apis essence is simple and production efficiency is high.
Detailed description of the invention
Below with reference to specific embodiment, the present invention is further illustrated.
The embodiment of the present invention a kind of Venenum apis essence, including following components: melittin, propolis, Radix Notoginseng,
Olibanum, Flos Carthami, Radix Aconiti Preparata, Radix Aconiti Kusnezoffii Preparata, Rhizoma Homalomenae, Fructus Zanthoxyli, Herba Lycopodii, Herba speranskiae tuberculatae, Borneolum Syntheticum, Camphor tree
Brain, Mentholum, capsaicin, chlorhexidine acetate, Oleum Cinnamomi, Oleum Terebinthinae and wintergreen oil, wherein, described
The quality of melittin is 0.10～0.20g, and the quality of described propolis is 2～4g, the quality of described Radix Notoginseng
Being 2～4g, the quality of described Olibanum is 9～11g, and the quality of described Flos Carthami is 9～11g, described system
The quality of Radix Aconiti is 5～7g, and the quality of described Radix Aconiti Kusnezoffii Preparata is 5～7g, the quality of described Rhizoma Homalomenae be 8～
12g, the quality of described Fructus Zanthoxyli is 8～12g, and the quality of described Herba Lycopodii is 35～45g, described deep
The quality of grass is 25～35g, and the quality of described Borneolum Syntheticum is 1～5g, and the quality of described Camphora is 1～4g,
The quality of described Mentholum is 0.2～0.8g, and the quality of described capsaicin is 1～4g, described acetic acid chlorine
The most fixed quality is 2～5g, and the quality of described Oleum Cinnamomi is 8～13g, and described terebinthine quality is
35～45g, the quality of described wintergreen oil is 90～110g.
As the preferred implementation of above-described embodiment, the quality of described melittin is 0.15g, described honeybee
The quality of glue is 3g, and the quality of described Radix Notoginseng is 3g, and the quality of described Olibanum is 10g, described Flos Carthami
Quality be 10g, the quality of described Radix Aconiti Preparata is 6g, and the quality of described Radix Aconiti Kusnezoffii Preparata is 6g, described thousand
The quality of Rhizoma Homalomenae is 10g, and the quality of described Fructus Zanthoxyli is 10g, and the quality of described Herba Lycopodii is 40g, institute
The quality stating Herba speranskiae tuberculatae is 30g, and the quality of described Borneolum Syntheticum is 3g, and the quality of described Camphora is 2g, institute
The quality stating Mentholum is 0.5g, and the quality of described capsaicin is 2g, the quality of described chlorhexidine acetate
For 3g, the quality of described Oleum Cinnamomi is 10g, and described terebinthine quality is 40g, described wintergreen oil
Quality is 100g.
As the preferred implementation of above-described embodiment, the quality of described melittin is 0.18g, described honeybee
The quality of glue is 3.5g, and the quality of described Radix Notoginseng is 2.5g, and the quality of described Olibanum is 10.5g, institute
The quality stating Flos Carthami is 9.5g, and the quality of described Radix Aconiti Preparata is 5.5g, and the quality of described Radix Aconiti Kusnezoffii Preparata is
6.5g, the quality of described Rhizoma Homalomenae is 11g, and the quality of described Fructus Zanthoxyli is 9g, the quality of described Herba Lycopodii
For 42g, the quality of described Herba speranskiae tuberculatae is 28g, and the quality of described Borneolum Syntheticum is 4g, the quality of described Camphora
For 3g, the quality of described Mentholum is 0.65g, and the quality of described capsaicin is 2.5g, described acetic acid
The quality of hibitane is 4g, and the quality of described Oleum Cinnamomi is 12g, and described terebinthine quality is 42g,
The quality of described wintergreen oil is 95g.
The present invention also provides for the preparation method of a kind of Venenum apis essence, comprises the following steps:
Prepared by S1, Venenum apis venon extractor: glass plate one piece, sieve-like wire netting, plastic sheeting one, directly
Convertor transformer one, supply socket, wire netting one side is covered with plastic sheeting, plastic sheeting is towards glass plate
Direction distance 0.5cm wooden frame is fixed；Transformer frequency response is connected with wire netting；
S2, collection Venenum apis: collecting Venenum apis and select the autumn in summer to replace season, now Apis Venenum apis content is high, no
Will again cloudy day blowing and raining time gather venom, sky temperature is not less than 20 DEG C, and when taking poison, operator put on anti-
Protect clothes, protective footwear, wear protective mast；Venon extractor is placed on beehive mouth, treat Apis climb full wire netting and
Being evenly distributed, turn on the power switch, voltage progressively heightens 30 volts, is energized 15 seconds, has a power failure 5 seconds,
So circulation operation 15 minutes, Apis tailpin punctures plastic sheeting, and Venenum apis is come glass plate
On, close power supply after 15 minutes, stop 10～15 minutes, after Apis is emotionally stable, take glass away
Plate；
S3, the purification of Venenum apis: include that magazins' layout separates with anaphylactogen, wherein, described magazins' layout
For by the Venenum apis vinegar solution containing impurity with in the pure water of 5～10 times, filter with middling speed qualitative filter paper,
Remove the insoluble matter impurity such as dust, Venenum apis solution add the acetone of 2 times, makes Venenum apis Precipitation,
Full Venenum apis is obtained after isolating moisture drying；Described anaphylactogen is separated into ultrafiltration and decomposes histamine and phospholipase
A2, uses acetate fiber and cellulose nitrate mixing filter membrane, filters Venenum apis solution and histamine is removed, then exist
Venenum apis solution instills qs glycerin, phospholipase A2 is resolved into phospholipid and fatty acid；
It is standby that S4, propolis Polyethylene Glycol are completely dissolved preparation propolis liquid；
S5, Chinese herbal medicine is mixed according to formula proportion, decoct with moisture 2 times, merge decocting liquid, add
Borneolum Syntheticum, Camphora, Mentholum, capsaicin and chlorhexidine acetate 200 mesh filtering and concentrating to 1000ml；
S6, that Venenum apis adds Tween 80 is appropriate, mixes with propolis liquid, propolis mixed liquor and traditional Chinese drug inspissation
Liquid mixing for standby use；
S7, above-mentioned mixed liquor is added wintergreen oil etc. oily mixed liquor Venenum apis essence finished product.
As the preferred implementation of above-described embodiment, when described step S1 collects Venenum apis, mouth to be worn
Cover protection, cleans glass plate with 2 times of white vinegars, makes Venenum apis avoid with air contact and aoxidizes, Venenum apis solution
Load in vial and store.
A kind of Venenum apis essence that the present invention provides, has the following characteristics that
1, safe and scientific exploitation Venenum apis, is applied to scrape therapy physical therapy, tcm health preserving, releases for extensive patients
Disease is painful, solves honeybee and treats the Apis brought and sting the misery of thorn and the risk brought because of allergy.Venenum apis refines
Product use safer, more convenient.Make melissotherapy be popularized, allow extensive patients be benefited, bring benefit to the people；
2, melissotherapy is often stung by acupuncture point and is stung, and Apis tailpin is taken out of together with abdominal part poison capsule, sacrificial
One Apis life of domestic animal, not only operates extremely difficult grasp, and this medical procedure lacks hommization；If sacrificing honey
Honeybee quantity is too many, also can affect other produce yield such as crops, Mel；
3, utilize natural resources, increase the extra earning of beekeeper, allow more beekeeper get rich；
4, low-carbon environment-friendly, develops green medicine, is applied to biomedicine field, economizes on resources, wound effect
The benefit turnover of profit and tax, benefits the nation and the people.
It is last it is noted that above-described each embodiment is merely to illustrate the present invention's
Technical scheme, is not intended to limit；Although the present invention having been carried out detailed with reference to previous embodiment
Illustrate, it will be understood by those within the art that: previous embodiment still can be remembered by it
The technical scheme carried is modified, or wherein part or all of technical characteristic is carried out equivalent；
And these amendments or replacement, do not make the essence of appropriate technical solution depart from various embodiments of the present invention skill
The scope of art scheme.
Claims (5)
1. a Venenum apis essence, it is characterised in that: include following components: melittin, propolis, Radix Notoginseng,
Olibanum, Flos Carthami, Radix Aconiti Preparata, Radix Aconiti Kusnezoffii Preparata, Rhizoma Homalomenae, Fructus Zanthoxyli, Herba Lycopodii, Herba speranskiae tuberculatae, Borneolum Syntheticum,
Camphora, Mentholum, capsaicin, chlorhexidine acetate, Oleum Cinnamomi, Oleum Terebinthinae and wintergreen oil, wherein,
The quality of described melittin is 0.10～0.20g, and the quality of described propolis is 2～4g, described Radix Notoginseng
Quality be 2～4g, the quality of described Olibanum is 9～11g, and the quality of described Flos Carthami is 9～11g,
The quality of described Radix Aconiti Preparata is 5～7g, and the quality of described Radix Aconiti Kusnezoffii Preparata is 5～7g, described Rhizoma Homalomenae
Quality is 8～12g, and the quality of described Fructus Zanthoxyli is 8～12g, and the quality of described Herba Lycopodii is 35～45g,
The quality of described Herba speranskiae tuberculatae is 25～35g, and the quality of described Borneolum Syntheticum is 1～5g, the matter of described Camphora
Amount is 1～4g, and the quality of described Mentholum is 0.2～0.8g, the quality of described capsaicin be 1～
4g, the quality of described chlorhexidine acetate is 2～5g, and the quality of described Oleum Cinnamomi is 8～13g, described
Terebinthine quality is 35～45g, and the quality of described wintergreen oil is 90～110g.
Venenum apis the most according to claim 1 essence, it is characterised in that the quality of described melittin is
0.15g, the quality of described propolis is 3g, and the quality of described Radix Notoginseng is 3g, and the quality of described Olibanum is
10g, the quality of described Flos Carthami is 10g, and the quality of described Radix Aconiti Preparata is 6g, the matter of described Radix Aconiti Kusnezoffii Preparata
Amount is 6g, and the quality of described Rhizoma Homalomenae is 10g, and the quality of described Fructus Zanthoxyli is 10g, described Herba Lycopodii
Quality be 40g, the quality of described Herba speranskiae tuberculatae is 30g, and the quality of described Borneolum Syntheticum is 3g, described Camphor tree
The quality of brain is 2g, and the quality of described Mentholum is 0.5g, and the quality of described capsaicin is 2g, institute
The quality stating chlorhexidine acetate is 3g, and the quality of described Oleum Cinnamomi is 10g, described terebinthine quality
For 40g, the quality of described wintergreen oil is 100g.
Venenum apis the most according to claim 1 essence, it is characterised in that the quality of described melittin is
0.18g, the quality of described propolis is 3.5g, and the quality of described Radix Notoginseng is 2.5g, the matter of described Olibanum
Amount is 10.5g, and the quality of described Flos Carthami is 9.5g, and the quality of described Radix Aconiti Preparata is 5.5g, described
The quality of Radix Aconiti Kusnezoffii Preparata is 6.5g, and the quality of described Rhizoma Homalomenae is 11g, and the quality of described Fructus Zanthoxyli is 9g,
The quality of described Herba Lycopodii is 42g, and the quality of described Herba speranskiae tuberculatae is 28g, and the quality of described Borneolum Syntheticum is
4g, the quality of described Camphora is 3g, and the quality of described Mentholum is 0.65g, the matter of described capsaicin
Amount is 2.5g, and the quality of described chlorhexidine acetate is 4g, and the quality of described Oleum Cinnamomi is 12g, institute
Stating terebinthine quality is 42g, and the quality of described wintergreen oil is 95g.
4. the preparation method of a Venenum apis essence, it is characterised in that comprise the following steps:
Prepared by S1, Venenum apis venon extractor: glass plate one piece, sieve-like wire netting, plastic sheeting one, directly
Convertor transformer one, supply socket, wire netting one side is covered with plastic sheeting, plastic sheeting is towards glass
Plate direction distance 0.5cm wooden frame is fixed；Transformer frequency response is connected with wire netting；
S2, collection Venenum apis: collecting Venenum apis and select the autumn in summer to replace season, now Apis Venenum apis content is high, no
Will gather venom when cloudy day blowing and raining, sky temperature is not less than 20 DEG C, and when taking poison, operator put on
Protective garment, protective footwear, wear protective mast；Venon extractor is placed on beehive mouth, treats that Apis climbs full metal
Net and being evenly distributed, turns on the power switch, and voltage progressively heightens 30 volts, is energized 15 seconds, has a power failure
5 seconds, so circulation operation 15 minutes, Apis tailpin punctured plastic sheeting, Venenum apis is arranged
On a glass, close power supply after 15 minutes, stop 10～15 minutes, after Apis is emotionally stable,
Take glass plate away；
S3, the purification of Venenum apis: include that magazins' layout separates with anaphylactogen, wherein, described magazins' layout
For the Venenum apis vinegar solution containing impurity is diluted, by middling speed qualitative filter paper mistake with the pure water of 5～10 times
Filter, removes the insoluble matter impurity such as dust, adds the acetone of 2 times, make Venenum apis precipitate in Venenum apis solution
Separate out, after isolating moisture drying, obtain full Venenum apis；Described anaphylactogen be separated into ultrafiltration decompose histamine and
Phospholipase A2, uses acetate fiber and cellulose nitrate mixing filter membrane, filters Venenum apis solution and histamine is removed
Go, in Venenum apis solution, then instill qs glycerin, phospholipase A2 is resolved into phospholipid and fatty acid；
It is standby that S4: propolis Polyethylene Glycol is completely dissolved preparation propolis liquid；
S5: mixed according to formula proportion by Chinese herbal medicine, decocts for 2 times with moisture, merges decocting liquid, add
Borneolum Syntheticum, Camphora, Mentholum, capsaicin and chlorhexidine acetate 200 mesh filtering and concentrating to 1000ml；
S6: Venenum apis adding Tween 80 in right amount, mix with propolis liquid, propolis mixed liquor is dense with Chinese medicine
Contracting liquid mixing for standby use；
S7: above-mentioned mixed liquor is added in wintergreen oil mixed liquor Venenum apis essence finished product.
The preparation method of Venenum apis the most according to claim 4 essence, it is characterised in that described step
When collecting Venenum apis in S1, wear masks protection, cleans glass plate with 2 times of white vinegars, makes Venenum apis avoid
Aoxidize with air contact, Venenum apis solution is loaded in vial and stores.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109350658A (en) * | 2018-12-14 | 2019-02-19 | 北京蜂珍科技开发有限公司 | A kind of bee venom Chinese medicine scraping oil for rheumatic arthritis |
| CN109364166A (en) * | 2018-12-14 | 2019-02-22 | 北京蜂珍科技开发有限公司 | A kind of water-soluble scraping agent containing bee venom |
| CN114010577A (en) * | 2021-11-04 | 2022-02-08 | 佛山蜂缘生物科技有限公司 | Preparation method and application method of repairing liquid combining wasp venom and wintergreen oil |
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| CN114010577A (en) * | 2021-11-04 | 2022-02-08 | 佛山蜂缘生物科技有限公司 | Preparation method and application method of repairing liquid combining wasp venom and wintergreen oil |
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