CN101088514A - Bee venom refining process - Google Patents

Abstract

The bee venom refining process includes the following steps: dissolving coarse bee venom in alcohol to form suspension, filtering to eliminate propolis, honey and other impurity, centrifuging the filtrate and eliminating liquid phase, dewatering the precipitate with anhydrous alcohol, leaching the precipitate in water at 10+/-2 deg.c for 12-15 hr, precipitating the leached liquid with alcohol, eliminating water solution of alcohol, extracting the precipitate in ammonium hydroxide and n-butanol solution, distilling the extracted liquid, concentrating and freeze drying to obtain refined bee venom. The refined bee venom may be used in different clinical research and application fields, including preparing electrophoresis level melittin.

Description

The process for purification of Venenum apis

Technical field

The present invention relates to the process for purification of Apis poison, particularly utilize refining Venenum apis to extract the refining of its Venenum apis of melittin.

Background technology

Apis poison (Bee Venom is called for short Venenum apis) is to be stored in the malicious capsule after being produced by bee venom, a kind of venom that when the Apis irriate, goes out from the chela faller gill, and Venenum apis is the compounding substances of a kind of pharmacological and biochemical activity high complexity, and extensive use is medically being arranged.But the clinical practice of Venenum apis is still very limited up till now, and reason mainly is that the composition of Venenum apis is numerous, refining purifying technique complexity, and cost is higher; Highly finished product still can not satisfy the clinical application requirements that waits special dimension, particularly can not satisfy the specification requirement of producing electrophoresis level melittin.

Summary of the invention

The objective of the invention is to overcome the deficiency that prior art exists, a kind of process for purification of Venenum apis is provided, the refining Venenum apis that this method obtains, the specification requirement that can satisfy clinical research and application and extract electrophoresis level melittin.

Process for purification provided by the invention is that thick Venenum apis is dissolved in impurity such as the propolis in the removal Venenum apis, Mel in the alcoholic solution, adopts the solvent extraction method again, obtains refining Venenum apis, and feature of the present invention is:

1) thick Venenum apis is added 95% ethanol and make suspension, filter and remove impurity, liquid phase is removed in the centrifugal back of filtrate, and precipitate adds the dehydrated alcohol dehydration, again the precipitate that proposes is added entry in 10 ± 2 ℃ of following lixiviate 12～15hr;

2) lixiviating solution ethanol precipitation is removed ethanol water, extracts in ammonium hydroxide that the precipitate adding is 0.15～0.2 mole and the butanol solution, and reject precipitate, distillation extraction liquid, concentrate carry out lyophilization and promptly obtain refining Venenum apis.

This method can be made with extra care the Venenum apis product by mass production, and craft science is reasonable, not only can satisfy the needs of more areas such as clinical research and application, can also satisfy the specification requirement of preparation electrophoresis level melittin.

Below by technology diagram and embodiment the present invention is described in detail.

Embodiment:

Get thick Venenum apis 100g, add 500ml95% ethanol and make suspension, jolting 4hr filters insoluble matter impurity such as removing the Apis sting; Remove liquid phase behind filtrate centrifugal (3000r/min) 20min, precipitate adds the dehydration of 300ml dehydrated alcohol, uses the 300mlPH=6.4 distilled water in 10 ± 2 ℃ of following lixiviate 12～15hr, reject precipitate the precipitate that proposes again, the lixiviating solution ethanol precipitation is removed ethanol water; Dissolve in precipitate adding 6000ml0.15 moles of hydrogen amine-oxides and the 4000ml butanol solution, jolting 2hr carries out liquid-solid separation, the reject precipitation, and extract distills the recovery n-butyl alcohol, and concentrate carries out lyophilization and promptly obtains refining Venenum apis.

Claims (2)

1, a kind of process for purification of Venenum apis is that thick Venenum apis is dissolved in impurity such as the propolis in the removal Venenum apis, Mel in the alcoholic solution, adopts the solvent extraction method again, obtains refining Venenum apis, and feature of the present invention is:

1) thick Venenum apis is added 95% ethanol and make suspension, filter and remove impurity, liquid phase is removed in the centrifugal back of filtrate, and precipitate adds the dehydrated alcohol dehydration, again the precipitate that proposes is added entry in 10 ± 2 ℃ of following lixiviate 12～15hr;

2) lixiviating solution ethanol precipitation is removed ethanol water, extracts in ammonium hydroxide that the precipitate adding is 0.15～0.2 mole and the butanol solution, and reject precipitate, distillation extraction liquid, concentrate carry out lyophilization and promptly obtain refining Venenum apis.

2, in accordance with the method for claim 1, it is characterized in that lixiviate is 6.4 with the PH of distilled water.