CN105861572A - Method for promoting excess sludge carbon source conversion and in-situ synthesis by rhamnolipid biosurfactant - Google Patents
Method for promoting excess sludge carbon source conversion and in-situ synthesis by rhamnolipid biosurfactant Download PDFInfo
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Abstract
The invention relates to methods for promoting excess sludge carbon source conversion and in-situ synthesis by surfactants, in particular to a method for promoting excess sludge carbon source conversion and in-situ synthesis by a rhamnolipid biosurfactant, aiming to solve the problems that chemical surfactants in existing methods for promoting excess sludge anaerobic fermentation to produce acid are toxic and fail to degrade biologically to cause secondary pollution to excess sludge, and the chemical surfactants-lauryl sodium sulfate and sodium dodecyl benzene sulfonate are weak in excess sludge acidizing efficiency and poor in economy. The method includes firstly, preparing a sludge sample; secondly, adding rhamnolipid; thirdly, conducting anaerobic fermentation. The rhamnolipid biosurfactant used in the method is good in biodegradability and environment friendliness so as to eliminate secondary pollution of toxicity to the excess sludge gradually, has a great promoting effect on fermentation and acid production of the excess sludge and is capable of achieving in-situ synthesis during anaerobic fermentation.
Description
Technical field
The present invention relates to surfactant and promote that excess sludge carbon source converts and the method for fabricated in situ.
Background technology
The sustained and rapid development of national economy causes city domestic sewage discharge capacity to increase year by year, strength disposal also gesture
Must be along with increase.By the end of the year 2008, the built municipal sewage plant run of going into operation, the whole nation reaches 1519
Seat, wherein the sewage treatment plant of 86.2% uses activated sludge process, the average treatment water yield about 6699.8 ten thousand m3/d。
But in sewage disposal process, but produce substantial amounts of excess sludge.Within 2008, national excess sludge generation amount is high
Reaching 18,000,000 tons/year (80% moisture content), quickly increase with the speed of annual 10% afterwards, it processes disposal
Expense typically constitutes the 20～50% of the total running cost of sewage disposal, and even 70%.Municipal sludge contain a large amount of
Organic matter, compares and abandons as garbage, the most potential becomes a kind of valuable but cheap resource.Cause
How this, combine social benefit, economic benefit and environmental benefit and utilize the organic matter in mud to be modern substantially
The required new direction considered of rear specific resistance to filtration technology development.
Existing researcher finds, chemical surfactant can promote the dissolving of particulate organic matter in excess sludge,
Reduce the activity of methanogen, so that the intermediate product VFAs of excess sludge anaerobic fermentation process is able to greatly
Amount accumulation.But chemical surfactant promotes that the chemical surface in the method for excess sludge anaerobic fermentation and acid production is lived
There is toxicity in property agent, and the most biodegradable excess sludge causes secondary pollution, chemical surfactant 12
Alkyl sodium sulfate and dodecylbenzene sodium sulfonate convert the more weak and less economical (need of usefulness to the carbon source of excess sludge
Constantly put into) problem.
Summary of the invention
The present invention solves in the method that existing chemical surfactant promotes excess sludge anaerobic fermentation and acid production
Chemical surfactant there is toxicity, and the most biodegradable excess sludge is caused secondary pollution, chemistry table
It is more weak that face activating agent sodium lauryl sulphate and dodecylbenzene sodium sulfonate convert usefulness to the carbon source of excess sludge
And less economical problem, and excess sludge carbon source converts and former to provide rhamnolipid biological surface activator to promote
The method of position synthesis.
Rhamnolipid biological surface activator promote excess sludge anaerobic fermentation and acid production method, specifically according to
Lower step is carried out:
One, excess sludge is put in container carry out natural subsidence, sedimentation time under conditions of temperature is 4 DEG C
It is 24～30h, supernatant of then draining, obtain mud sample；
Two, putting in reaction bulb by mud sample, add rhamnolipid, wherein the dosage of rhamnolipid is
0.005～0.10g/gVSS；
Three, after reaction bulb being driven oxygen and filling nitrogen 10min, seal reaction bulb, put in air bath shaking table with
100～110rpm/min rotating speeds, carry out anaerobic fermentation, and fermentation temperature is 35～38 DEG C, fermentation time is 96～192h,
Complete rhamnolipid biological surface activator and promote that excess sludge carbon source converts and the process of fabricated in situ.
The invention has the beneficial effects as follows: rhamnolipid biological surface activator promotes excess sludge anaerobic fermentation and acid production
Method in rhamnolipid biological surface activator there is good biodegradability and environment friendly, reach
To gradually eliminating the toxicity secondary pollution to excess sludge；Rhamnolipid biological surface activator promotes excess sludge
The method of anaerobic fermentation and acid production has bigger facilitation to excess sludge fermentation and acid, and rhamnolipid can
Synthesize at sludge anaerobic sweat situ, when rhamnolipid, sodium lauryl sulphate and detergent alkylate sulphur
Acid sodium, dosage is 0.04g/gVSS, when fermentation time is 96h, adds rhamnolipid biological surface and lives
Property agent, compared with adding sodium lauryl sulphate and dodecylbenzene sodium sulfonate chemical surfactant experimental group, is waved
Concentration of turning sour is respectively increased 3.62 times and 1.22 times；When fermentation time 96h, rhamnolipid dosage is 0.04
During g/gTSS and 0.10g/gTSS, in liquid phase, rhamnolipid concentration reaches 1312mg/L and 2382mg/L, is
Initial 1.49 times (880mg/L) and 1.08 times (2199mg/L) put into of fermentation.
The inventive method is used for promoting that excess sludge carbon source converts and fabricated in situ.
Accompanying drawing explanation
Fig. 1 is contrast experiment one, embodiment one, embodiment two, embodiment three, embodiment four, embodiment five
Volatile acid concentration and the graph of a relation of fermentation time with embodiment six；WhereinRepresent waving of contrast experiment one
Concentration of turning sour and the relation curve of fermentation time,Represent volatile acid concentration and the fermentation time of embodiment one
Relation curve,Represent the volatile acid concentration of embodiment two and the relation curve of fermentation time,Generation
The volatile acid concentration of table embodiment three and the relation curve of fermentation time,Represent the volatile acid of embodiment four
Concentration and the relation curve of fermentation time,Represent the volatile acid concentration of embodiment five and the pass of fermentation time
It is curve,Represent the volatile acid concentration of embodiment six and the relation curve of fermentation time.
Fig. 2 is the volatile acid concentration of contrast experiment two, contrast experiment three, contrast experiment four and embodiment seven and sends out
The graph of a relation of ferment time；WhereinThe volatile acid concentration representing contrast experiment two is bent with the relation of fermentation time
Line,Represent the volatile acid concentration of contrast experiment three and the relation curve of fermentation time,Represent contrast
The volatile acid concentration of experiment four and the relation curve of fermentation time,Represent the volatile acid concentration of embodiment seven
Relation curve with fermentation time.
Fig. 3 is embodiment one, embodiment two, embodiment three, embodiment four, embodiment five, embodiment six and
The rhamnolipid content of contrast experiment one and the graph of a relation of fermentation time；WhereinRepresent embodiment one
The relation curve of rhamnolipid concentration and fermentation time,Represent the rhamnolipid concentration of embodiment two
With the relation block diagram of fermentation time,Represent rhamnolipid concentration and the fermentation time of embodiment three
Relation block diagram,Represent the rhamnolipid concentration of embodiment four and the relation block diagram of fermentation time,Represent the rhamnolipid concentration of embodiment five and the relation block diagram of fermentation time,Represent
The rhamnolipid concentration of embodiment six and the relation block diagram of fermentation time,Represent contrast experiment's one
Rhamnolipid concentration and the relation block diagram of fermentation time.
Detailed description of the invention
Technical solution of the present invention is not limited to the detailed description of the invention of act set forth below, also includes each specific embodiment party
Combination in any between formula.
Detailed description of the invention one: present embodiment rhamnolipid biological surface activator promotes that excess sludge carbon source turns
Change and the method for fabricated in situ, specifically follow the steps below:
One, excess sludge is put in container carry out natural subsidence, sedimentation time under conditions of temperature is 4 DEG C
It is 24～30h, supernatant of then draining, obtain mud sample；
Two, putting in reaction bulb by mud sample, add rhamnolipid, wherein the dosage of rhamnolipid is
0.005～0.10g/gVSS；
Three, after reaction bulb being driven oxygen and filling nitrogen 10min, seal reaction bulb, put in air bath shaking table with
100～110rpm/min rotating speeds, carry out anaerobic fermentation, and fermentation temperature is 35～38 DEG C, fermentation time is 96～192h,
Complete rhamnolipid biological surface activator and promote the process of excess sludge anaerobic fermentation and acid production.
Detailed description of the invention two: present embodiment is unlike detailed description of the invention one: in step one during sedimentation
Between be 25～29h.Other is identical with detailed description of the invention one.
Detailed description of the invention three: present embodiment is unlike one of detailed description of the invention one to two: step 2
The injected volume of middle rhamnolipid is 0.02～0.08g/gVSS.Other is identical with one of detailed description of the invention one to two.
Detailed description of the invention four: present embodiment is unlike one of detailed description of the invention one to three: step 3
The rotating speed of middle air bath shaking table is 102～108rpm/min.Other is identical with one of detailed description of the invention one to three.
Detailed description of the invention five: present embodiment is unlike one of detailed description of the invention one to four: step 3
Middle fermentation temperature is 36～37 DEG C, fermentation time is 100～190h.One of other and detailed description of the invention one to four
Identical.
Detailed description of the invention six: present embodiment is unlike one of detailed description of the invention one to five: residue dirt
Containing Pseudomonas alba in mud.Other is identical with one of detailed description of the invention one to five.
Following example and contrast experiment is used to verify beneficial effects of the present invention:
Embodiment one:
The present embodiment rhamnolipid biological surface activator promotes excess sludge carbon source to convert and the side of fabricated in situ
Method, specifically follows the steps below:
One, measure 800mL excess sludge, the excess sludge measured is put in container at the bar that temperature is 4 DEG C
Carrying out natural subsidence under part, the sedimentation time is 24h, supernatant of then draining, and obtains mud sample, wherein dirty
Mud sample TSS be 12.1g/L, VSS be 6.5g/L；
Two, with 500mL serum bottle as reaction bulb, measure 300mL mud sample and put in reaction bulb, throw
Adding rhamnolipid, wherein the dosage of rhamnolipid is 0.005g/gVSS；
Three, after reaction bulb being driven oxygen and filling nitrogen 10min, seal reaction bulb, put in air bath shaking table with
100rpm/min rotating speed, carries out anaerobic fermentation, and fermentation temperature is 35 DEG C, fermentation time is 192h, completes Mus
Lee's glycolipid biosurfactant promotes the process of excess sludge anaerobic fermentation and acid production.
Wherein excess sludge takes from Harbin peace water process second pond, containing Pseudomonas alba in excess sludge.
Embodiment two:
The present embodiment rhamnolipid biological surface activator promotes excess sludge carbon source to convert and the side of fabricated in situ
Method, specifically follows the steps below:
One, measure 800mL excess sludge, the excess sludge measured is put in container at the bar that temperature is 4 DEG C
Carrying out natural subsidence under part, the sedimentation time is 24h, supernatant of then draining, and obtains mud sample, wherein dirty
Mud sample TSS be 12.1g/L, VSS be 6.5g/L；
Two, with 500mL serum bottle as reaction bulb, measure 300mL mud sample and put in reaction bulb, throw
Adding rhamnolipid, wherein the dosage of rhamnolipid is 0.02g/gVSS；
Three, after reaction bulb being driven oxygen and filling nitrogen 10min, seal reaction bulb, put in air bath shaking table with
100rpm/min rotating speed, carries out anaerobic fermentation, and fermentation temperature is 35 DEG C, fermentation time is 192h, completes Mus
Lee's glycolipid biosurfactant promotes the process of excess sludge anaerobic fermentation and acid production.
Wherein excess sludge takes from Harbin peace water process second pond, containing Pseudomonas alba in excess sludge.
Embodiment three:
The present embodiment rhamnolipid biological surface activator promotes excess sludge carbon source to convert and the side of fabricated in situ
Method, specifically follows the steps below:
One, measure 800mL excess sludge, the excess sludge measured is put in container at the bar that temperature is 4 DEG C
Carrying out natural subsidence under part, the sedimentation time is 24h, supernatant of then draining, and obtains mud sample, wherein dirty
Mud sample TSS be 12.1g/L, VSS be 6.5g/L；
Two, with 500mL serum bottle as reaction bulb, measure 300mL mud sample and put in reaction bulb, throw
Adding rhamnolipid, wherein the dosage of rhamnolipid is 0.04g/gVSS；
Three, after reaction bulb being driven oxygen and filling nitrogen 10min, seal reaction bulb, put in air bath shaking table with
100rpm/min rotating speed, carries out anaerobic fermentation, and fermentation temperature is 35 DEG C, fermentation time is 192h, completes Mus
Lee's glycolipid biosurfactant promotes the process of excess sludge anaerobic fermentation and acid production.
Wherein excess sludge takes from Harbin peace water process second pond, containing Pseudomonas alba in excess sludge.
Embodiment four:
The present embodiment rhamnolipid biological surface activator promotes excess sludge carbon source to convert and the side of fabricated in situ
Method, specifically follows the steps below:
One, measure 800mL excess sludge, the excess sludge measured is put in container at the bar that temperature is 4 DEG C
Carrying out natural subsidence under part, the sedimentation time is 24h, supernatant of then draining, and obtains mud sample, wherein dirty
Mud sample TSS be 12.1g/L, VSS be 6.5g/L；
Two, with 500mL serum bottle as reaction bulb, measure 300mL mud sample and put in reaction bulb, throw
Adding rhamnolipid, wherein the dosage of rhamnolipid is 0.06g/gVSS；
Three, after reaction bulb being driven oxygen and filling nitrogen 10min, seal reaction bulb, put in air bath shaking table with
100rpm/min rotating speed, carries out anaerobic fermentation, and fermentation temperature is 35 DEG C, fermentation time is 192h, completes Mus
Lee's glycolipid biosurfactant promotes the process of excess sludge anaerobic fermentation and acid production.
Wherein excess sludge takes from Harbin peace water process second pond, containing Pseudomonas alba in excess sludge.
Embodiment five:
The present embodiment rhamnolipid biological surface activator promotes excess sludge carbon source to convert and the side of fabricated in situ
Method, specifically follows the steps below:
One, measure 800mL excess sludge, the excess sludge measured is put in container at the bar that temperature is 4 DEG C
Carrying out natural subsidence under part, the sedimentation time is 24h, supernatant of then draining, and obtains mud sample, wherein dirty
Mud sample TSS be 12.1g/L, VSS be 6.5g/L；
Two, with 500mL serum bottle as reaction bulb, measure 300mL mud sample and put in reaction bulb, throw
Adding rhamnolipid, wherein the dosage of rhamnolipid is 0.08g/gVSS；
Three, after reaction bulb being driven oxygen and filling nitrogen 10min, seal reaction bulb, put in air bath shaking table with
100rpm/min rotating speed, carries out anaerobic fermentation, and fermentation temperature is 35 DEG C, fermentation time is 192h, completes Mus
Lee's glycolipid biosurfactant promotes the process of excess sludge anaerobic fermentation and acid production.
Wherein excess sludge takes from Harbin peace water process second pond, containing Pseudomonas alba in excess sludge.
Embodiment six:
The present embodiment rhamnolipid biological surface activator promotes excess sludge carbon source to convert and the side of fabricated in situ
Method, specifically follows the steps below:
One, measure 800mL excess sludge, the excess sludge measured is put in container at the bar that temperature is 4 DEG C
Carrying out natural subsidence under part, the sedimentation time is 24h, supernatant of then draining, and obtains mud sample, wherein dirty
Mud sample TSS be 12.1g/L, VSS be 6.5g/L；
Two, with 500mL serum bottle as reaction bulb, measure 300mL mud sample and put in reaction bulb, throw
Adding rhamnolipid, wherein the dosage of rhamnolipid is 0.10g/gVSS；
Three, after reaction bulb being driven oxygen and filling nitrogen 10min, seal reaction bulb, put in air bath shaking table with
100rpm/min rotating speed, carries out anaerobic fermentation, and fermentation temperature is 35 DEG C, fermentation time is 192h, completes Mus
Lee's glycolipid biosurfactant promotes the process of excess sludge anaerobic fermentation and acid production.
Wherein excess sludge takes from Harbin peace water process second pond, containing Pseudomonas alba in excess sludge.
Embodiment seven:
The present embodiment rhamnolipid biological surface activator promotes excess sludge carbon source to convert and the side of fabricated in situ
Method, specifically follows the steps below:
One, measure 800mL excess sludge, the excess sludge measured is put in container at the bar that temperature is 4 DEG C
Carrying out natural subsidence under part, the sedimentation time is 24h, supernatant of then draining, and obtains mud sample, wherein dirty
Mud sample TSS be 23.4g/L, VSS be 14.0g/L；
Two, with 500mL serum bottle as reaction bulb, measure 300mL mud sample and put in reaction bulb, throw
Adding rhamnolipid, wherein the dosage of rhamnolipid is 0.04g/gVSS；
Three, after reaction bulb being driven oxygen and filling nitrogen 10min, seal reaction bulb, put in air bath shaking table with
100rpm/min rotating speed, carries out anaerobic fermentation, and fermentation temperature is 35 DEG C, fermentation time is 192h, completes Mus
Lee's glycolipid biosurfactant promotes the process of excess sludge anaerobic fermentation and acid production.
Wherein excess sludge takes from Harbin peace water process second pond, containing Pseudomonas alba in excess sludge.
Contrast experiment one:
This contrast experiment does not adds the method for surfactant excess sludge anaerobic fermentation and acid production, specifically by following
Step completes:
One, measure 800mL excess sludge, the excess sludge measured is put in container at the bar that temperature is 4 DEG C
Carrying out natural subsidence under part, the sedimentation time is 24h, supernatant of then draining, and obtains mud sample, wherein dirty
Mud sample TSS be 12.1g/L, VSS be 6.5g/L；
Two, with 500mL serum bottle as reaction bulb, measure 300mL mud sample and put in reaction bulb；
Three, after reaction bulb being driven oxygen and filling nitrogen 10min, seal reaction bulb, put in air bath shaking table with
100rpm/min rotating speed, carries out anaerobic fermentation, and fermentation temperature is 35 DEG C, fermentation time is 192h, completes to remain
The process of remaining sludge anaerobic fermentation and acid production.
Wherein excess sludge takes from Harbin peace water process second pond, containing Pseudomonas alba in excess sludge.
Contrast experiment two:
This contrast experiment lauryl sodium sulfate surfactant promotes the method for excess sludge anaerobic fermentation and acid production,
Specifically follow the steps below:
One, measure 800mL excess sludge, the excess sludge measured is put in container at the bar that temperature is 4 DEG C
Carrying out natural subsidence under part, the sedimentation time is 24h, supernatant of then draining, and obtains mud sample, wherein dirty
Mud sample TSS be 23.4g/L, VSS be 14.0g/L；
Two, with 500mL serum bottle as reaction bulb, measure 300mL mud sample and put in reaction bulb, throw
Adding sodium lauryl sulphate, wherein the dosage of sodium lauryl sulphate is 0.04g/gVSS；
Three, after reaction bulb being driven oxygen and filling nitrogen 10min, seal reaction bulb, put in air bath shaking table with
100rpm/min rotating speed, carries out anaerobic fermentation, and fermentation temperature is 35 DEG C, fermentation time is 192h, completes ten
Sodium dialkyl sulfate surfactant promotes the process of excess sludge anaerobic fermentation and acid production.
Wherein excess sludge takes from Harbin peace water process second pond, containing Pseudomonas alba in excess sludge.
Contrast experiment three:
This contrast experiment dodecylbenzene sodium sulfonate surfactant promotes the side of excess sludge anaerobic fermentation and acid production
Method, specifically follows the steps below:
One, measure 800mL excess sludge, the excess sludge measured is put in container at the bar that temperature is 4 DEG C
Carrying out natural subsidence under part, the sedimentation time is 24h, supernatant of then draining, and obtains mud sample, wherein dirty
Mud sample TSS be 23.4g/L, VSS be 14.0g/L；
Two, with 500mL serum bottle as reaction bulb, measure 300mL mud sample and put in reaction bulb, throw
Adding dodecylbenzene sodium sulfonate, wherein the dosage of dodecylbenzene sodium sulfonate is 0.04g/gVSS；
Three, after reaction bulb being driven oxygen and filling nitrogen 10min, seal reaction bulb, put in air bath shaking table with
100rpm/min rotating speed, carries out anaerobic fermentation, and fermentation temperature is 35 DEG C, fermentation time is 192h, completes ten
Dialkyl benzene sulfonic acids natrium surfactant promotes the process of excess sludge anaerobic fermentation and acid production.
Wherein excess sludge takes from Harbin peace water process second pond, containing Pseudomonas alba in excess sludge.
Contrast experiment four:
This contrast experiment does not adds the method for surfactant excess sludge anaerobic fermentation and acid production, specifically by following
Step completes:
One, measure 800mL excess sludge, the excess sludge measured is put in container at the bar that temperature is 4 DEG C
Carrying out natural subsidence under part, the sedimentation time is 24h, supernatant of then draining, and obtains mud sample, wherein dirty
Mud sample TSS be 23.4g/L, VSS be 14.0g/L；
Two, with 500mL serum bottle as reaction bulb, measure 300mL mud sample and put in reaction bulb；
Three, after reaction bulb being driven oxygen and filling nitrogen 10min, seal reaction bulb, put in air bath shaking table with
100rpm/min rotating speed, carries out anaerobic fermentation, and fermentation temperature is 35 DEG C, fermentation time is 192h, completes to remain
The process of remaining sludge anaerobic fermentation and acid production.
Wherein excess sludge takes from Harbin peace water process second pond, containing Pseudomonas alba in excess sludge.
Fig. 1 is embodiment one, embodiment two, embodiment three, embodiment four, embodiment five, embodiment six and
The volatile acid concentration of contrast experiment one and the graph of a relation of fermentation time；WhereinRepresent the volatilization of embodiment one
Acid concentration and the relation curve of fermentation time,Represent volatile acid concentration and the fermentation time of embodiment two
Relation curve,Represent the volatile acid concentration of embodiment three and the relation curve of fermentation time,Represent
The volatile acid concentration of embodiment four and the relation curve of fermentation time,The volatile acid representing embodiment five is dense
Degree and the relation curve of fermentation time,Represent the volatile acid concentration of embodiment six and the relation of fermentation time
Curve,Represent the volatile acid concentration of contrast experiment one and the relation curve of fermentation time.From figure permissible
Find out, compared with not adding surfactant excess sludge anaerobic fermentation volatile acid concentration, add rhamnolipid
Embodiment, volatile acid concentration all has raising in various degree, illustrates that excess sludge is acidified by adding of rhamnolipid
Usefulness has obvious facilitation, and volatile acid concentration increases along with the increase of rhamnolipid dosage, but throws
It is inconspicuous that dosage increases trend after 0.04g/gVSS, and rhamnolipid dosage the most conveniently is
0.04g/gVSS, when fermentation time is 96h, volatile acid concentration all reaches the highest 3840mgCOD/L, is not throw
Add 4.24 times of surfactant volatile acid concentration.
Fig. 2 is the volatile acid concentration of contrast experiment two, contrast experiment three, contrast experiment four and embodiment seven and sends out
The graph of a relation of ferment time；WhereinRepresent the volatile acid concentration of contrast experiment two and the relation of fermentation time
Curve,Represent the volatile acid concentration of contrast experiment three and the relation curve of fermentation time,It is right to represent
Than volatile acid concentration and the relation curve of fermentation time of experiment four,The volatile acid representing embodiment seven is dense
Degree and the relation curve of fermentation time.It can be seen that relative to adding chemical surfactant dodecane
Base sodium sulfate and dodecylbenzene sodium sulfonate, adding rhamnolipid has bigger promotion to excess sludge fermentation and acid
Effect.When fermentation time is 96h, add rhamnolipid, sodium lauryl sulphate and dodecylbenzene sodium sulfonate
Experimental group, volatile acid concentration is respectively 5972mgCOD/L, 4890mgCOD/L and 1648mgCOD/L, phase
For do not add surfactant excess sludge anaerobic fermentation volatile acid concentration compare be respectively increased 4.00 times, 3.28
Times and 1.10 times.And add rhamnolipid biological surface activator and add sodium lauryl sulphate and dodecane
Base benzene sulfonic acid sodium salt chemical surfactant experimental group is compared, and volatile acid concentration is respectively increased 3.62 times and 1.22 times.
Fig. 3 is embodiment one, embodiment two, embodiment three, embodiment four, embodiment five, embodiment six and
The rhamnolipid content of contrast experiment one and the graph of a relation of fermentation time；WhereinRepresent embodiment one
The relation curve of rhamnolipid concentration and fermentation time,Represent the rhamnolipid concentration of embodiment two
With the relation block diagram of fermentation time,Represent rhamnolipid concentration and the fermentation time of embodiment three
Relation block diagram,Represent the rhamnolipid concentration of embodiment four and the relation block diagram of fermentation time,Represent the rhamnolipid concentration of embodiment five and the relation block diagram of fermentation time,Represent
The rhamnolipid concentration of embodiment six and the relation block diagram of fermentation time,Represent contrast experiment's one
Rhamnolipid concentration and the relation block diagram of fermentation time.From figure 3, it can be seen that rhamnolipid concentration is whole
Not degraded in sweat, and promoted.When fermentation time 96h, rhamnolipid dosage is
During 0.04g/gTSS and 0.10g/gTSS, in liquid phase, rhamnolipid concentration reaches 1312mg/L and 2382mg/L,
It is initial 1.49 times (880mg/L) and 1.08 times (2199mg/L) put into of fermentation.
Claims (6)
1. rhamnolipid biological surface activator promotes excess sludge carbon source to convert and the method for fabricated in situ, it is characterised in that rhamnolipid biological surface activator promotes what the method for the conversion of excess sludge carbon source and fabricated in situ specifically followed the steps below:
One, being put into by excess sludge in container and carry out natural subsidence under conditions of temperature is 4 DEG C, the sedimentation time is 24 ~ 30h, and supernatant of then draining obtains mud sample；
Two, putting in reaction bulb by mud sample, add rhamnolipid, wherein the dosage of rhamnolipid is 0.005 ~ 0.10g/gVSS；
Three, after reaction bulb being driven oxygen and filling nitrogen 10min, seal reaction bulb, put in air bath shaking table with 100 ~ 110rpm/min rotating speed, carry out anaerobic fermentation, fermentation temperature is 35 ~ 38 DEG C, fermentation time is 96 ~ 192h, completes rhamnolipid biological surface activator and promotes the process of excess sludge anaerobic fermentation and acid production.
The method that the most according to claim 1, rhamnolipid biological surface activator promotes excess sludge anaerobic fermentation and acid production, it is characterised in that in step one, the sedimentation time is 25 ~ 29h.
The method that the most according to claim 2, rhamnolipid biological surface activator promotes excess sludge anaerobic fermentation and acid production, it is characterised in that in step 2, the injected volume of rhamnolipid is 0.02 ~ 0.08 g/gVSS.
The method that the most according to claim 3, rhamnolipid biological surface activator promotes excess sludge anaerobic fermentation and acid production, it is characterised in that in step 3, the rotating speed of air bath shaking table is 102 ~ 108rpm/min.
The method that the most according to claim 4, rhamnolipid biological surface activator promotes excess sludge anaerobic fermentation and acid production, it is characterised in that in step 3 fermentation temperature be 36 ~ 37 DEG C, fermentation time be 100 ~ 190h.
The most according to claim 5, rhamnolipid biological surface activator promotes excess sludge carbon source to convert and the method for fabricated in situ, it is characterised in that containing Pseudomonas alba in excess sludge.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106350548A (en) * | 2016-11-25 | 2017-01-25 | 太原理工大学 | Method for achieving conversion of micromolecular carbon sources of sludge through optimizing straw conditioning manner |
| CN109293189A (en) * | 2018-11-30 | 2019-02-01 | 江门市邑凯环保服务有限公司 | A method of promoting sludge hydrolysis, acidification |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102796764A (en) * | 2012-08-06 | 2012-11-28 | 哈尔滨工业大学 | Method for producing acid through promoting anaerobic fermentation of residual sludge by virtue of rhamnolipid biosurfactant |
| CN103757062A (en) * | 2014-01-10 | 2014-04-30 | 同济大学 | Method for promoting anaerobic fermentation acid production of excess sludge through surfactin |
| CN103773817A (en) * | 2014-01-10 | 2014-05-07 | 同济大学 | Method for promoting residual sludge to be anaerobically fermented to produce acid by utilizing tea saponin |
-
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102796764A (en) * | 2012-08-06 | 2012-11-28 | 哈尔滨工业大学 | Method for producing acid through promoting anaerobic fermentation of residual sludge by virtue of rhamnolipid biosurfactant |
| CN103757062A (en) * | 2014-01-10 | 2014-04-30 | 同济大学 | Method for promoting anaerobic fermentation acid production of excess sludge through surfactin |
| CN103773817A (en) * | 2014-01-10 | 2014-05-07 | 同济大学 | Method for promoting residual sludge to be anaerobically fermented to produce acid by utilizing tea saponin |
Non-Patent Citations (1)
| Title |
|---|
| 王家玲主编: "《环境微生物学（第二版）》", 31 January 2004 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106350548A (en) * | 2016-11-25 | 2017-01-25 | 太原理工大学 | Method for achieving conversion of micromolecular carbon sources of sludge through optimizing straw conditioning manner |
| CN106350548B (en) * | 2016-11-25 | 2019-11-01 | 太原理工大学 | The method that the optimization quenched mode of stalk realizes the conversion of sludge small molecule carbon source |
| CN109293189A (en) * | 2018-11-30 | 2019-02-01 | 江门市邑凯环保服务有限公司 | A method of promoting sludge hydrolysis, acidification |
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