CN105860957A - Preparation method of oxidized graphene composite for drug carrier - Google Patents

Preparation method of oxidized graphene composite for drug carrier Download PDF

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CN105860957A
CN105860957A CN201610301469.8A CN201610301469A CN105860957A CN 105860957 A CN105860957 A CN 105860957A CN 201610301469 A CN201610301469 A CN 201610301469A CN 105860957 A CN105860957 A CN 105860957A
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sio
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CN105860957B (en
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张艳华
肖巍
邓莹
陈慧
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Shenzhen Texin Interface Technology Co ltd
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Chongqing University of Arts and Sciences
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    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
    • C09K11/02—Use of particular materials as binders, particle coatings or suspension media therefor
    • C09K11/025—Use of particular materials as binders, particle coatings or suspension media therefor non-luminescent particle coatings or suspension media
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y20/00—Nanooptics, e.g. quantum optics or photonic crystals
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y40/00—Manufacture or treatment of nanostructures
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    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
    • C09K11/08—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials
    • C09K11/54—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials containing zinc or cadmium
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
    • C09K11/08—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials
    • C09K11/65—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials containing carbon

Abstract

Disclosed is a preparation method of oxidized graphene composite for drug carrier. The preparation method includes preparing ZnO quantum dots and Fe3O4 nanoparticles of mesoporous structure respectively, wrapping these two kinds of nanoparticles with a SiO2 shell layer and then aminating them. The composite nanoparticles aminated are grafted to the pre-carboxylation surface of the oxidized graphene through covalent crosslinking to obtain magnetic and fluorescent oxidized graphene composite. The oxidized graphene composite prepared has evident magnetic performance. In addition, the oxidized graphene composite has large specific area, and its good performance in fluorescence may help trace delivery path of itself as the drug carrier.

Description

A kind of preparation method of the graphene oxide composite material for pharmaceutical carrier
The present patent application be on 09 26th, 2014 applying date, application number 201410502642.1 patent of invention point Case application.
Technical field
The present invention relates to technical field of inorganic nanometer material, be specifically related to a kind of stannic oxide/graphene nano composite and system Preparation Method.
Background technology
Graphene oxide is as the research aspect of pharmaceutical carrier: first Graphene has specific surface area and the pi-electron of superelevation Conjugated system.Graphene oxide can pass through pi-pi accumulation and hydrogen bond action carrying medicament, and can hang with pole fine particulate form Float on aqueous solution or physiological environment system.Research shows, graphene oxide can be absorbed by cell and not have obvious cytotoxicity, The small-molecule drug of some aromatics had superpower absorbability, the graphene oxide that organic molecule is surface-functionalized to it There is during as pharmaceutical carrier other materials unrivaled superelevation carrying drug ratio, anticancer drugs, doxorubicin medicine carrying on Graphene Rate is up to 400%, and far above general nano material carrier, meanwhile, the graphene oxide good water solubility of functionalization also has good life Thing safety, is especially suitable for doing pharmaceutical carrier.Magnetic nanoparticle is loaded into magnetic targeted drug prepared by graphenic surface Carrier, has great application potential in actual disease is treated.Research about magnetic nano-particle modified graphene is existing A small amount of report, these magnetic nanoparticle/graphene composite materials are expected at magnetic targeted medicine carrying, bio-separation, nuclear magnetic resonance, And obtain in fields such as removing in sewage condensed ring pollutant and to be widely applied.More than in research, used magnetic particle is all real Heart Fe3O4Nano-particle, wherein magnetic particle the most only plays the effect making material have magnetic property, and nanoparticle The structure of son does not the most embody significantly effect.
Quantum dot is used as fluorescent marker spike to be observed living cells or the structure of tissue and activity or even carries out living animal imaging And report the most in a large number for following the trail of the research of drug particles trend in vivo and effect.Amount used in these reports Son point uses collochemistry method to synthesize in organic system mostly, the quantum dot poorly water-soluble therefore obtained, and synthesize The oxygen group elements binary compound of quantum dot composition mainly Cd and nucleocapsid structure thereof.Cd is a kind of heavy metal unit having severe toxicity Element, the quantum dot containing Cd easily discharges Cd during biomarker2+And damage biological body.Although the preparation of cadmium content point and knot The detection of structure, performance is the most highly developed, and numerous studies have shown that employing surface modification technology or aqueous synthesis method can To be substantially improved the stability containing Cd quantum dot and safety, but this kind of quantum dot still can not be completely eliminated in use process Middle meeting decomposites free Cd2+Probability, therefore the biological safety of cadmium content point does not the most obtain basic solution.Cause This, for biological safety and application angle, actively develop hypotoxicity or the preparation of bio-compatibility quanta point material, structure Research with performance will become the developing direction of following quanta point material.At present, be applied to bioluminescence detection hypotoxicity or Bio-compatibility quantum dot is mainly ZnS, ZnSe and ZnO quantum dot.ZnO, as the quantum dot of biological fluorescent labelling, relatively contains The bio-toxicity of the quantum dot of Cd is less, for being test for animal and testing research person, the most fool proof.As safety A kind of advanced luminescent material nontoxic, cheap and good-quality, ZnO quantum dot, at basic research and actual application two aspects, has very Important value.
Summary of the invention
It is an object of the invention to provide a kind of magnetic fluorescent dual-function graphene oxide that can be used as target medicine carrier The preparation method of nano composite material.Specifically include following steps:
(1) preparation of graphene oxide
Powdered graphite is added H2SO4、K2S2O8、P2O5Mixed liquor in, 70 ~ 85 DEG C reaction 3 ~ 6h.Then dilute, and mistake Filter washing is to remove unnecessary acid.Gained pre-product is dried at room temperature for overnight, then the graphite of pre-oxidation is added dense H2SO4, It is gradually added KMnO under ice-water bath is protected4, this mixture enters in 85 ~ 95 DEG C after stirring reaction 30 min at 30 ~ 35 DEG C Row reaction 1 ~ 2h, rear dilute so that it is boiling reaction 20 ~ 40min, after being stirred for 1 ~ 2 hour, add water and the H of 30wt%2O2 Solution left standstill precipitates.Mixture rinses for several times with dilute hydrochloric acid after filtering, then rinses to neutral with water.Finally products therefrom is dialysed Within one week, purification removes remaining metal ion, the graphite oxide the most ultrasonic stripping 30 ~ 50min obtained, and obtains dense Degree is about 0.1 ~ 1mg mL-1Graphene oxide storing solution.Use 1-(3-dimethylamino-propyl)-3-ethyl carbodiimide And N-hydroxy-succinamide (NHS) makes the activated carboxylic at graphene oxide plane and edge (EDC).Described H2SO4、K2S2O8、 P2O5Mixed liquor, in the mass ratio of each component be: 10:1:1.
(2) mesoporous Fe3O4The preparation of magnetic nanoparticle
By Fe (NO under room temperature3) 3·9H2O is dissolved in ethylene glycol, is heated to forming dry Fe in 70 ~ 80 DEG C after stirring 3 hours (NO3)3Ethylene glycol gel.Fe (the NO that will be formed3)3Ethylene glycol gel is placed in horizontal pipe furnace 380 ~ 450 DEG C and carries out heat Naturally cool to room temperature after solution, product is dried with after pure water and ethanol wash respectively in 70 ~ 80 DEG C of drying baker.It is eventually adding Ferroso-ferric oxide is modified by appropriate TMA, rear ultrasonic, shake up and disperse to obtain the mesoporous of variable concentrations in deionized water Fe3O4Nanoparticle storing solution.
(3) there is the preparation of the adjustable ZnO quantum dot of fluorescence color
Under room temperature, by Zn (OAc)2·2H2O and LiOH H2O is dissolved in TEG according to mol ratio 2:3, is stirred continuously until solution Luminescence.After according to certain mol proportion B(Mg:Zn) by Mg (OAc)2·4H2O joins in above-mentioned solution, super under the conditions of ice-water bath Sonication 2 ~ 5min, purification, obtain the ZnO quantum dot of different glow color.Described mol ratio B (Mg:Zn)=(0 ~ 0.1).
(4) (mesoporous Fe3O4-ZnO quantum dot)@SiO2Preparation
Hexamethylene, triton x-100 and hexanol are added beaker according to volume ratio 4:1:1, adds after stirring 15 ~ 30min The Fe of 200ul3O4Nano-particle solution and 800ulZnO quantum dot solution (concentration: 1 ~ 10mmol/l), after stirring 10 ~ 30min, Add 120ul NH4OH and 120ul PDDA solution (0.075%V/V).Add in system after continuing stirring 20min 80ul TEOS, continues under the conditions of lucifuge to be stirred vigorously 12 ~ 24h.The last acetone adding 20 ~ 30ml again in this system, obtains (mesoporous Fe3O4-ZnO quantum dot)@SiO2Composite nanoparticle, finally uses APS and THPMP that compound particle surface is carried out ammonia Base functionalization.Described Fe3O4Nano-particle solution concentration is 1 ~ 10mmol/l, and ZnO quantum dot solution concentration is 1 ~ 10mmol/l.
(5) preparation of the graphene oxide composite material of magnetic fluorescent dual-function
By amidized (mesoporous Fe3O4-ZnO quantum dot)@SiO2Nano composite granules is incorporated into carboxylic by the method for covalent cross-linking The surface of graphene oxide of base, obtains the graphene oxide composite material with magnetic fluorescent dual-function.
Compared with prior art, there is advantages that
The graphene nanocomposite material that the present invention prepares is by mesoporous Fe3O4The magnetic of nano-particle, Hole bottom cavity blast, Gao Bibiao Area and the fluorescence property of ZnO quantum dot and the extra specific surface area of graphene oxide, good bio-compatibility etc. blend, Can be as a kind of new drug carrier with magnetic target tropism, fluorescence and high drug load.Target product is possible not only to realize The targeting transport of medicine, it is also possible to medicament transport process is carried out fluorescent tracing, has great medical application and is worth.
Accompanying drawing explanation
Fig. 1 is the SEM figure of the magnetic fluorescent dual-function stannic oxide/graphene nano composite prepared.
Fig. 2 is the TEM figure of the magnetic fluorescent dual-function stannic oxide/graphene nano composite prepared.
Fig. 3 is the PL spectrogram of the magnetic fluorescent dual-function stannic oxide/graphene nano composite prepared.
Fig. 4 is the magnetization curve figure of the magnetic fluorescent dual-function stannic oxide/graphene nano composite prepared.
Detailed description of the invention
Below by embodiment, the present invention is further described.
Embodiment 1
(1) preparation of graphene oxide
2g graphite powder is added the dense H of 25g2SO4、2.5g K2S2O8With 2.5g P2O5Mixed liquor in, 80 DEG C reaction 3h.Then add 500ml water dilutes, and filtration washing is to remove unnecessary acid.Gained pre-product is dried at room temperature for overnight, then will pre-oxidation Graphite add the dense H of 100ml2SO4In, under ice-water bath is protected, it is gradually added 13g KMnO4, this mixture stirs at 35 DEG C Carry out reacting 2h, rear dilute so that it is boiling reaction 30min in 85 DEG C after reacting 30 min, after being stirred for 1 hour, add 500ml water and the H of 20ml and 30%2O2Solution left standstill precipitates.Mixture rinses 5 times with the dilute hydrochloric acid of 1:10 after filtering, then uses water Rinse to neutral.One week purification of finally being dialysed by products therefrom removes remaining metal ion, and the graphite oxide obtained is in room temperature Under the conditions of ultrasonic stripping 30min, be configured to concentration and be about the graphene oxide storing solution of 0.5mg/ml.To 50ml graphite oxide The dispersion liquid of alkene adds 1 mg 1-(3-dimethylamino-propyl)-3-ethyl carbodiimide (EDC) and 0.5 mg N-hydroxyl amber Amber acid imide (NHS), stirs 30min, makes the activated carboxylic at GO plane and edge.
(2) mesoporous Fe3O4The preparation of magnetic nanoparticle
By 0.02mol Fe (NO under room temperature3) 3·9H2O is dissolved in 25ml ethylene glycol, is heated to shape in 80 DEG C after stirring 3 hours Fe (the NO of Cheng Gan3)3Ethylene glycol gel.Fe (the NO that 1g is formed3)3Ethylene glycol gel is placed in horizontal pipe furnace, with 10 DEG C/heating rate to 400 DEG C of min naturally cools to room temperature after keeping 12h, by product respectively with after pure water and ethanol wash 12h it is dried in 70 DEG C of drying baker.Dried product will add appropriate TMA, be dispersed in after ultrasonic 1min 10ml go from In sub-water, obtain the mesoporous Fe that concentration is about 10 mmol/L3O4Nanoparticle storing solution.
(3) there is the preparation of the adjustable ZnO quantum dot of fluorescence color
Under room temperature, by 0.002molZn (OAc)2·2H2The LiOH H of O and 0.003mol2O is dissolved in 40ml TEG, persistently stirs Mix until solution luminescence, purification, be configured to the ZnO quantum dot of 10mmol/l.
(4) (mesoporous Fe3O4-ZnO quantum dot)@SiO2Preparation
15ml hexamethylene, 3.6ml triton x-100 and 3.6ml hexanol are added beaker, after stirring 15min, adds 200ul's The Fe of concentration 10mmol/l3O4The ZnO quantum dot solution of nano-particle solution and 800ul concentration 10mmol/l, stirs 10min After, add 120ul NH4OH and 120ul PDDA solution (0.075%V/V).Add in system after continuing stirring 20min 80ul TEOS, continues under the conditions of lucifuge to be stirred vigorously 24h.The last acetone adding 20ml again in this system, obtains (mesoporous Fe3O4-ZnO quantum dot)@SiO2Composite nanoparticle.30ulAPS and 80ulTHPMP is joined (the mesoporous Fe of preparation3O4 - ZnO quantum dot)@SiO2In composite nanoparticle, stir 24h, finally obtain (the mesoporous Fe of surface amination3O4-ZnO quantum Point)@SiO2Composite nanoparticle.
(5) preparation of the graphene oxide composite material of magnetic fluorescent dual-function
By 20ml(4) in amino functional (the mesoporous Fe that obtains3O4-ZnO quantum dot)@SiO2Composite nanoparticle joins The GO dispersion liquid of 50ml concentration 0.5mg/ml activated carboxylic reacts 2 h, makes (mesoporous Fe3O4-ZnO quantum dot)@SiO2Compound The amino of nanoparticle surface forms amido link with the carboxyl covalent cross-linking of GO.Then by step with Magneto separate is cleaned multiple times Suddenly, target product magnetic fluorescence polyfunctional GO composite nanometer particle is obtained.
Embodiment 2
(1) preparation of graphene oxide
2g graphite powder is added the dense H of 25g2SO4、2.5g K2S2O8With 2.5g P2O5Mixed liquor in, 70 DEG C reaction 5h.Then add 500ml water dilutes, and filtration washing is to remove unnecessary acid.Gained pre-product is dried at room temperature for overnight, then will pre-oxidation Graphite add the dense H of 150ml2SO4In, under ice-water bath is protected, it is gradually added 20g KMnO4, this mixture stirs at 30 DEG C Carry out reacting 2h, rear dilute so that it is boiling reaction 30min in 85 DEG C after reacting 30 min, after being stirred for 1 hour, add The H of 500ml water 20ml and 30%2O2Solution left standstill precipitates.Mixture rinses 5 times with the dilute hydrochloric acid of 1:10 after filtering, then rushes with water It is washed till neutrality.One week purification of finally being dialysed by products therefrom removes remaining metal ion, and the graphite oxide obtained is at room temperature bar Under part, ultrasonic stripping 30min, is configured to concentration and is about the graphene oxide storing solution of 0.2 mg/ml.To 50ml graphite oxide The dispersion liquid of alkene adds 0.5 mg 1-(3-dimethylamino-propyl)-3-ethyl carbodiimide (EDC) and 0.2 mg N-hydroxyl Butanimide (NHS), stirs 30min, makes the activated carboxylic at GO plane and edge.
(2) mesoporous Fe3O4The preparation of magnetic nanoparticle
By 0.02mol Fe (NO under room temperature3) 3·9H2O is dissolved in 25ml ethylene glycol, is heated to shape in 70 DEG C after stirring 3 hours Fe (the NO of Cheng Gan3)3Ethylene glycol gel.Fe (the NO that 1g is formed3)3Ethylene glycol gel is placed in horizontal pipe furnace, with 10 DEG C/heating rate to 400 DEG C of min naturally cools to room temperature after keeping 12h, by product respectively with after pure water and ethanol wash 12h it is dried in 70 DEG C of drying baker.Dried product will add appropriate TMA, be dispersed in after ultrasonic 1min 100ml go from In sub-water, obtain the mesoporous Fe that concentration is about 1 mmol/L3O4Nanoparticle storing solution.
(3) there is the preparation of the adjustable ZnO quantum dot of fluorescence color
Under room temperature, by 0.002molZn (OAc)2·2H2The LiOH H of O and 0.003mol2O is dissolved in 40ml TEG, persistently stirs Mix until solution luminescence.By the Mg (OAc) of 0.0002mol2·4H2O joins in above-mentioned solution, ultrasonic under the conditions of ice-water bath Process 5min, purification, be configured to the ZnO quantum dot of 10mmol/l.
(4) (mesoporous Fe3O4-ZnO quantum dot)@SiO2Preparation
15ml hexamethylene, 3.6ml triton x-100 and 3.6ml hexanol are added beaker, after stirring 15min, adds 200ul's The Fe of concentration 1mmol/l3O4The ZnO quantum dot solution of nano-particle solution and 800ul concentration 10mmol/l, stirs 10min After, add 120ul NH4OH and 120ul PDDA solution (0.075%V/V).Add in system after continuing stirring 20min 80ul TEOS, continues under the conditions of lucifuge to be stirred vigorously 24h.The last acetone adding 20ml again in this system, obtains (mesoporous Fe3O4-ZnO quantum dot)@SiO2Composite nanoparticle.30ulAPS and 80ulTHPMP is joined (the mesoporous Fe of preparation3O4 - ZnO quantum dot)@SiO2In composite nanoparticle, stir 24h, finally obtain (the mesoporous Fe of surface amination3O4-ZnO quantum Point)@SiO2Composite nanoparticle.
(5) preparation of the graphene oxide composite material of magnetic fluorescent dual-function
By 20ml(4) in amino functional (the mesoporous Fe that obtains3O4-ZnO quantum dot)@SiO2Composite nanoparticle solution adds In the activated carboxylic GO dispersion liquid of 50ml concentration 0.2 mg/ml, react 2 h, make (mesoporous Fe3O4-ZnO quantum dot)@SiO2Multiple The carboxyl covalent cross-linking of the amino and GO that close nanoparticle surface forms amido link.Then by step with Magneto separate is cleaned multiple times Suddenly, target product magnetic fluorescence polyfunctional GO composite nanometer particle is obtained.
Embodiment 3
(1) preparation of graphene oxide
2g graphite powder is added the dense H of 25g2SO4、2.5g K2S2O8With 2.5g P2O5Mixed liquor in, 80 DEG C reaction 3h.Then add 500ml water dilutes, and filtration washing is to remove unnecessary acid.Gained pre-product is dried at room temperature for overnight, then will pre-oxidation Graphite add the dense H of 100ml2SO4In, under ice-water bath is protected, it is gradually added 13g KMnO4, this mixture stirs at 35 DEG C Carry out reacting 2h, rear dilute so that it is boiling reaction 30min in 85 DEG C after reacting 30 min, after being stirred for 1 hour, add 500ml water and the H of 20ml and 30%2O2Solution left standstill precipitates.Mixture rinses 5 times with the dilute hydrochloric acid of 1:10 after filtering, then uses water Rinse to neutral.One week purification of finally being dialysed by products therefrom removes remaining metal ion, and the graphite oxide obtained is in room temperature Under the conditions of ultrasonic stripping 30min, be configured to concentration and be about the graphene oxide storing solution of 0.1mg/ml.To 50ml graphite oxide The dispersion liquid of alkene adds 2mg 1-(3-dimethylamino-propyl)-3-ethyl carbodiimide (EDC) and 0.1mg N-hydroxysuccinimidyl Acid imide (NHS), stirs 30min, makes the activated carboxylic at GO plane and edge.
(2) mesoporous Fe3O4The preparation of magnetic nanoparticle
By 0.02mol Fe (NO under room temperature3) 3·9H2O is dissolved in 25ml ethylene glycol, is heated to shape in 80 DEG C after stirring 3 hours Fe (the NO of Cheng Gan3)3Ethylene glycol gel.Fe (the NO that 1g is formed3)3Ethylene glycol gel is placed in horizontal pipe furnace, with 10 DEG C/heating rate to 400 DEG C of min naturally cools to room temperature after keeping 12h, by product respectively with after pure water and ethanol wash 12h it is dried in 70 DEG C of drying baker.Dried product will add appropriate TMA, be dispersed in after ultrasonic 1min 10ml go from In sub-water, obtain the mesoporous Fe that concentration is about 10 mmol/L3O4Nanoparticle storing solution.
(3) there is the preparation of the adjustable ZnO quantum dot of fluorescence color
Under room temperature, by 0.002molZn (OAc)2·2H2The LiOH H of O and 0.003mol2O is dissolved in 40ml TEG, persistently stirs Mix until solution luminescence, by the Mg (OAc) of 0.0001mol2·4H2O joins in above-mentioned solution, ultrasonic under the conditions of ice-water bath Process 5min, purification, be configured to the ZnO quantum dot of 10mmol/l
(4) (mesoporous Fe3O4-ZnO quantum dot)@SiO2Preparation
15ml hexamethylene, 3.6ml triton x-100 and 3.6ml hexanol are added beaker, after stirring 15min, adds 200ul's The Fe of concentration 10mmol/l3O4The ZnO quantum dot solution of nano-particle solution and 800ul concentration 10mmol/l, stirs 10min After, add 120ul NH4OH and 120ul PDDA solution (0.075%V/V).Add in system after continuing stirring 20min 80ul TEOS, continues under the conditions of lucifuge to be stirred vigorously 24h.The last acetone adding 20ml again in this system, obtains (mesoporous Fe3O4-ZnO quantum dot)@SiO2Composite nanoparticle.30ulAPS and 80ulTHPMP is joined (the mesoporous Fe of preparation3O4 - ZnO quantum dot)@SiO2In composite nanoparticle, stir 24h, finally obtain (the mesoporous Fe of surface amination3O4-ZnO quantum Point)@SiO2Composite nanoparticle.
(5) preparation of the graphene oxide composite material of magnetic fluorescent dual-function
By 20ml(4) in amino functional (the mesoporous Fe that obtains3O4-ZnO quantum dot)@SiO2Composite nanoparticle joins The GO dispersion liquid of 50ml concentration 0.1mg/ml activated carboxylic reacts 2 h, makes (mesoporous Fe3O4-ZnO quantum dot)@SiO2Compound The amino of nanoparticle surface forms amido link with the carboxyl covalent cross-linking of GO.Then by step with Magneto separate is cleaned multiple times Suddenly, target product magnetic fluorescence polyfunctional GO composite nanometer particle is obtained.

Claims (1)

1. the preparation method for the graphene oxide composite material of pharmaceutical carrier, it is characterised in that make as follows Standby:
(1) preparation of graphene oxide
2g graphite powder is added the dense H of 25g2SO4、2.5g K2S2O8With 2.5g P2O5Mixed liquor in, 80 DEG C reaction 3h, then add 500ml water dilutes, and filtration washing is to remove unnecessary acid, and gained pre-product is dried at room temperature for overnight, being subsequently adding 100ml Dense H2SO4In, under ice-water bath is protected, it is gradually added 13g KMnO4, this mixture stirs reaction about 30 min at 35 DEG C After in 85 DEG C carry out react 2h, rear dilute so that it is boiling reaction 30min, after being stirred for 1 hour, add 500ml water and The H of 20ml and 30%2O2Solution left standstill precipitation, mixture rinses 5 times with the dilute hydrochloric acid of 1:10 after filtering, then with in water flushing extremely Property, one week purification of finally being dialysed by products therefrom removes remaining metal ion, and the graphite oxide obtained surpasses at ambient temperature Sound peels off 30min, is configured to the graphene oxide storing solution that concentration is 0.5mg/ml, in the dispersion liquid of 50ml graphene oxide Add 1 mg 1-(3-dimethylamino-propyl)-3-ethyl carbodiimide (EDC) and 0.5 mg N-hydroxy-succinamide (NHS), stir 30min, make the activated carboxylic at GO plane and edge；
(2) mesoporous Fe3O4The preparation of magnetic nanoparticle
By 0.02mol Fe (NO under room temperature3) 3·9H2O is dissolved in 25ml ethylene glycol, is heated to shape in 80 DEG C after stirring 3 hours Fe (the NO of Cheng Gan3)3Ethylene glycol gel, the Fe (NO that 1g is formed3)3Ethylene glycol gel is placed in horizontal pipe furnace, with 10 DEG C/heating rate to 400 DEG C of min naturally cools to room temperature after keeping 12h, by product respectively with after pure water and ethanol wash In 70 DEG C of drying baker, it is dried 12h, by dried product adds TMA, is dispersed in after ultrasonic 1min in 10ml deionized water, Obtain the mesoporous Fe that concentration is about 10 mmol/L3O4Nanoparticle storing solution；
(3) there is the preparation of the adjustable ZnO quantum dot of fluorescence color
Under room temperature, by about 0.002molZn (OAc)2·2H2The LiOH H of O and 0.003mol2O is dissolved in 40ml TEG, holds Continuous stirring, until solution luminescence, purification, is configured to the ZnO quantum dot of 10mmol/l；
(4) (mesoporous Fe3O4-ZnO quantum dot)@SiO2Preparation
15ml hexamethylene, 3.6ml triton x-100 and 3.6ml hexanol are added beaker, after stirring 15min, adds 200ul's The Fe of concentration 10mmol/l3O4The ZnO quantum dot solution of nano-particle solution and 800ul concentration 10mmol/l, stirs 10min After, add 120ul NH4OH and 120ul PDDA solution (0.075%V/V), adds after continuing stirring 20min in system 80ul TEOS, continues under the conditions of lucifuge to be stirred vigorously 24h, adds the acetone of 20ml the most again in this system, obtain (mesoporous Fe3O4-ZnO quantum dot)@SiO2Composite nanoparticle, joins (the mesoporous Fe of preparation by 30ulAPS and 80ulTHPMP3O4 - ZnO quantum dot)@SiO2In composite nanoparticle, stir about 24h, finally obtain (the mesoporous Fe of surface amination3O4 -ZnO Quantum dot)@SiO2Composite nanoparticle；
(5) preparation of the graphene oxide composite material of magnetic fluorescent dual-function
By 20ml(4) in amino functional (the mesoporous Fe that obtains3O4-ZnO quantum dot)@SiO2Composite nanoparticle joins The GO dispersion liquid of 50ml concentration 0.5mg/ml activated carboxylic reacts 2 h, makes (mesoporous Fe3O4-ZnO quantum dot)@SiO2It is combined and receives The amino on rice corpuscles surface forms amido link with the carboxyl covalent cross-linking of GO, then by cleaning and the step of Magneto separate, obtains Target product magnetic fluorescence polyfunctional GO composite nanometer particle.
CN201610301469.8A 2014-09-26 2014-09-26 A kind of preparation method of graphene oxide composite material for pharmaceutical carrier Active CN105860957B (en)

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CN114806551A (en) * 2021-06-01 2022-07-29 四川大学 Graphene fluorescent nano material and preparation method and application thereof
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